MD12G1043400.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002533
Physical Location & Seq
Reverse (-)
4882052 .. 4883897
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1043400.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGCGGCTTGGCAGAGGCATCTGCAATCAGCTATCCGTCAAATTGGAAAAAGGGTTGAATGTAGTTACAACCCTCTAGCCAATTTCTCTTCTTCTTCCTCTTCTCGTTTAGAGGGTCTCTTGCCTTATTTCCAAACTCTTTGCAAACCATCTTCTCCAACCATTTCAAGGCCCCTTCATCAATATTTTCAGCAGTTGGGAATTTCGAGTTCAAAGAACTTGAGAGCAGAGGAAACACCTCTTCCATCTCCATTGACTCCTGTTTTGGCATCAAATACTGCAACGAATGAGGAGCACAAGCAGAAATCACTCACTAAACCTGCTAAAGTTCAAGCTATCTTAAAGGGAATAAAGCAGAGCCCTAAGAAGGTCAACTTGGTTGCTGCACTGGTGCGTGGTATGCGTGTCGAAGATGCATTGTTGCAGTTGCAAGTGACAGTAAAGCGAGCTTCAAAAACTGTGTACCAGGTCATTCATTCAGCCCGAGCAAATGCAACTCATAATCATGGGTTAGACCCAGACCGTCTCCTTGTTGTTTTTAACCTTCTTTGTTCTGCATTTTCTCCTTATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

192

Amino Acids

21.13

Weight (kDa)

10.1

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 112 - 178 1.5e-17 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 328
AciI CCGC 1 cut(s) 5
AcsI RAATTY 1 cut(s) 201
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 2 cut(s) 168, 367
AgsI TTSAA 5 cut(s) 59, 168, 213, 332, 453
AjnI CCWGG 1 cut(s) 465
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 3 cut(s) 32, 335, 449
AluI AGCT 3 cut(s) 32, 335, 449
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 122, 530
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 1 cut(s) 201
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
AspS9I GGNCC 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 483
BanII GRGCYC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 157, 253
BciT130I CCWGG 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 122, 530
BfaI CTAG 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 2 cut(s) 6, 384
BlsI GCNGC 2 cut(s) 7, 385
Bme1390I CCNGG 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 3 cut(s) 28, 278, 403
BpuEI CTTGAG 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 122
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 367
Bse1I ACTGG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 467
BseLI CCNNNNNNNGG 2 cut(s) 168, 367
BseNI ACTGG 1 cut(s) 393
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 370
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 483
BslI CCNNNNNNNGG 2 cut(s) 168, 367
BsmAI GTCTC 2 cut(s) 122, 530
BsmBI CGTCTC 1 cut(s) 530
BsnI GGCC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 297, 362
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 173
BspMI ACCTGC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 122
BsrI ACTGG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 3 cut(s) 439, 460, 524
Bst6I CTCTTC 3 cut(s) 94, 106, 246
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 363
BstMAI GTCTC 2 cut(s) 122, 530
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 171
CsiI ACCWGGT 1 cut(s) 465
Csp6I GTAC 1 cut(s) 463
CviAII CATG 1 cut(s) 506
CviJI RGCY 8 cut(s) 8, 32, 80, 172, 335, 360, 449, 482
CviKI_1 RGCY 8 cut(s) 8, 32, 80, 172, 335, 360, 449, 482
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 363
Eam1104I CTCTTC 3 cut(s) 94, 106, 246
EarI CTCTTC 3 cut(s) 94, 106, 246
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 1 cut(s) 509
FaiI YATR 3 cut(s) 401, 501, 507
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FatI CATG 1 cut(s) 505
Fnu4HI GCNGC 2 cut(s) 6, 384
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 2 cut(s) 6, 384
FspBI CTAG 1 cut(s) 77
GluI GCNGC 2 cut(s) 6, 384
HaeIII GGCC 1 cut(s) 172
Hin1II CATG 1 cut(s) 509
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 373, 463
Hpy8I GTNNAC 2 cut(s) 373, 463
HpyAV CCTTC 3 cut(s) 185, 361, 554
HpyCH4III ACNGT 3 cut(s) 439, 460, 524
HpyCH4V TGCA 9 cut(s) 25, 144, 281, 386, 416, 424, 430, 494, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 363
Hsp92II CATG 1 cut(s) 509
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 6 cut(s) 273, 333, 374, 452, 479, 531
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 3 cut(s) 28, 278, 403
MabI ACCWGGT 1 cut(s) 465
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 2 cut(s) 65, 433
MboII GAAGA 7 cut(s) 81, 84, 87, 93, 144, 233, 422
MhlI GDGCHC 2 cut(s) 297, 362
MluCI AATT 3 cut(s) 42, 82, 201
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 7 cut(s) 9, 84, 106, 109, 223, 249, 283
Mph1103I ATGCAT 1 cut(s) 418
MseI TTAA 2 cut(s) 341, 540
MslI CAYNNNNRTG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 467
MvaI CCWGG 1 cut(s) 467
MwoI GCNNNNNNNGC 1 cut(s) 400
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 433
NsiI ATGCAT 1 cut(s) 418
PkrI GCNGC 2 cut(s) 7, 385
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 1 cut(s) 171
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 504
SaqAI TTAA 2 cut(s) 341, 540
SatI GCNGC 2 cut(s) 6, 384
Sau96I GGNCC 1 cut(s) 171
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 2 cut(s) 297, 362
SetI ASST 8 cut(s) 34, 241, 322, 337, 372, 451, 471, 546
SexAI ACCWGGT 1 cut(s) 465
SfaNI GCATC 3 cut(s) 28, 278, 403
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 3 cut(s) 42, 82, 201
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 185
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 439, 460, 524
TaqI TCGA 2 cut(s) 206, 408
TasI AATT 3 cut(s) 42, 82, 201
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 341, 540
Tru9I TTAA 2 cut(s) 341, 540
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 2 cut(s) 167, 464
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 393
XapI RAATTY 1 cut(s) 201
XspI CTAG 1 cut(s) 77
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.