Rroxscaffold_6G00405140

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002533
Physical Location & Seq
Forward (+)
27574016 .. 27576428
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405140.1

Sequence Viewer

Length: 828 bp
ATGGCGGGTTGGCAGAGGCATCTCCACTCCTTAATCCGTCAAGTTGGGAAAACCCTCGAATCCAATTACACCCAATCTGCCTCCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCGCTTAGAAGCTGGTTTGCCTTATTTGCAGAGACTCTGCAGCAGACCATCTTCTCCTACCATTCCAAGGCCTCTTCATCGTTACTTTCAACAATTGGGGATTTCGAGTTCGAGGAACTTACTTGCAGAGGAACCGGTTTCATCTCCATTGACTCCTCTTTTGACATCAACTTCTGCAAAAGCTGAAGAGCACAACCAGAAATCACTTACTAAGCCTTCTACAGTTCAAGCTATCCTAAAGGGCATCAAACAGAGTCCTAAAAAGGTCAATTTGGTTGCTGCACTGGTTCGTGGCATGCGTGTTGAAGATGCATTGTTGCAGTTGCAAGTGACGGTAAAGAGAGCTGCAAAAACTGTATATCAGGTTATTCACTCAGCCAGAGCAAACGCAACTCATAATCATGGGTTGGATCCAGACCGTCTCCTTGTTGCTGAGGCATTTGTTGGAAAGGGATTCTACAAGAAAAGAATTTCCTACCATGCTAAAGGAAAATGTGGAGTCAAAATGAGACCAGAATGCCGACTGACAGTGGTAGTACGGGAGATTACCCCTGACGAGGAGGCCGAGATAGCCAAACTGAAAGTTAAGAATTTTGTTAAGCTCACCAAGCGGGAAAGAAGGCTTGTTCCTCATCAGCTGATTGAGACAACTTCAATTTGGGGCCGCAAAGGCAAGGGTTGCAATCATGAACCAAATGGTATCCCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

275

Amino Acids

30.48

Weight (kDa)

10.27

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 116 - 219 1.3e-30 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 727, 781
AclWI GGATC 2 cut(s) 521, 534
AcsI RAATTY 2 cut(s) 585, 706
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 1 cut(s) 654
AfiI CCNNNNNNNGG 2 cut(s) 183, 673
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 4 cut(s) 206, 344, 422, 771
AloI GAACNNNNNNTCC 2 cut(s) 208, 240
AluBI AGCT 6 cut(s) 128, 299, 347, 461, 718, 754
AluI AGCT 6 cut(s) 128, 299, 347, 461, 718, 754
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 4 cut(s) 142, 542, 619, 755
AlwI GGATC 2 cut(s) 521, 534
AoxI GGCC 3 cut(s) 185, 678, 778
ApeKI GCWGC 3 cut(s) 156, 395, 461
ApoI RAATTY 2 cut(s) 585, 706
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AsiGI ACCGGT 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 778
AsuHPI GGTGA 1 cut(s) 712
BamHI GGATCC 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 168, 382, 448
BccI CCATC 1 cut(s) 172
BciVI GTATCC 1 cut(s) 827
BcoDI GTCTC 4 cut(s) 142, 542, 619, 755
BfmI CTRYAG 2 cut(s) 154, 336
BfuI GTATCC 1 cut(s) 827
BglI GCCNNNNNGGC 1 cut(s) 786
BisI GCNGC 4 cut(s) 157, 396, 462, 781
BlsI GCNGC 4 cut(s) 158, 397, 463, 782
BmgT120I GGNCC 1 cut(s) 778
BmiI GGNNCC 3 cut(s) 249, 528, 779
BmsI GCATC 3 cut(s) 28, 369, 415
Bpu10I CCTNAGC 1 cut(s) 549
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 673
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 182
BseLI CCNNNNNNNGG 2 cut(s) 183, 673
BseMII CTCAG 2 cut(s) 504, 540
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 2 cut(s) 261, 689
BseXI GCAGC 3 cut(s) 168, 382, 448
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 3 cut(s) 187, 680, 780
BshTI ACCGGT 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 183, 673
BsmAI GTCTC 4 cut(s) 142, 542, 619, 755
BsmBI CGTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 3 cut(s) 187, 680, 780
Bso31I GGTCTC 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 1 cut(s) 526
BspACI CCGC 3 cut(s) 5, 727, 781
BspANI GGCC 3 cut(s) 187, 680, 780
BspCNI CTCAG 2 cut(s) 503, 541
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 3 cut(s) 249, 528, 779
BspMAI CTGCAG 1 cut(s) 158
BspPI GGATC 2 cut(s) 521, 534
BspQI GCTCTTC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 619
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 1 cut(s) 526
BssT1I CCWWGG 1 cut(s) 182
Bst4CI ACNGT 5 cut(s) 340, 451, 472, 536, 646
Bst6I CTCTTC 2 cut(s) 195, 297
BstAPI GCANNNNNTGC 1 cut(s) 795
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 4 cut(s) 121, 327, 490, 549
BstKTI GATC 1 cut(s) 529
BstMAI GTCTC 4 cut(s) 142, 542, 619, 755
BstMBI GATC 1 cut(s) 526
BstMWI GCNNNNNNNGC 5 cut(s) 142, 686, 724, 786, 795
BstNSI RCATGY 1 cut(s) 415
BstSFI CTRYAG 2 cut(s) 154, 336
BstV1I GCAGC 3 cut(s) 168, 382, 448
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuI GTATCC 1 cut(s) 827
BsuRI GGCC 3 cut(s) 187, 680, 780
BtsIMutI CAGTG 2 cut(s) 398, 651
Cac8I GCNNGC 1 cut(s) 413
CciI TCATGA 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 778
Csp6I GTAC 1 cut(s) 653
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 5 cut(s) 412, 518, 596, 803, 825
CviQI GTAC 1 cut(s) 653
DdeI CTNAG 4 cut(s) 121, 327, 490, 549
DpnI GATC 1 cut(s) 528
DpnII GATC 1 cut(s) 526
Eam1104I CTCTTC 2 cut(s) 195, 297
EarI CTCTTC 2 cut(s) 195, 297
Eco130I CCWWGG 1 cut(s) 182
Eco147I AGGCCT 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 619
Eco57I CTGAAG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 5 cut(s) 415, 521, 599, 806, 828
FaiI YATR 7 cut(s) 413, 475, 513, 519, 597, 804, 826
FatI CATG 5 cut(s) 411, 517, 595, 802, 824
FauI CCCGC 1 cut(s) 720
Fnu4HI GCNGC 4 cut(s) 157, 396, 462, 781
Fsp4HI GCNGC 4 cut(s) 157, 396, 462, 781
GluI GCNGC 4 cut(s) 157, 396, 462, 781
HaeIII GGCC 3 cut(s) 187, 680, 780
HapII CCGG 1 cut(s) 251
Hin1II CATG 5 cut(s) 415, 521, 599, 806, 828
HinfI GANTC 6 cut(s) 59, 150, 268, 370, 570, 615
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 712
Hpy188III TCNNGA 2 cut(s) 530, 803
HpyAV CCTTC 2 cut(s) 342, 729
HpyCH4III ACNGT 5 cut(s) 340, 451, 472, 536, 646
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 686, 724, 786, 795
HpyF3I CTNAG 4 cut(s) 121, 327, 490, 549
Hsp92II CATG 5 cut(s) 415, 521, 599, 806, 828
Kzo9I GATC 1 cut(s) 526
LguI GCTCTTC 1 cut(s) 297
LpnPI CCDG 9 cut(s) 114, 264, 326, 386, 464, 508, 543, 642, 681
Lsp1109I GCAGC 3 cut(s) 168, 382, 448
LweI GCATC 3 cut(s) 28, 369, 415
MaeIII GTNAC 2 cut(s) 197, 445
MalI GATC 1 cut(s) 528
MboI GATC 1 cut(s) 526
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 6 cut(s) 64, 209, 385, 585, 706, 771
MlyI GAGTC 4 cut(s) 144, 262, 379, 624
MmeI TCCRAC 2 cut(s) 504, 541
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 3 cut(s) 32, 702, 714
MslI CAYNNNNRTG 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 1 cut(s) 251
MunI CAATTG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 638
MwoI GCNNNNNNNGC 5 cut(s) 142, 686, 724, 786, 795
NdeII GATC 1 cut(s) 526
NlaIII CATG 5 cut(s) 415, 521, 599, 806, 828
NlaIV GGNNCC 3 cut(s) 249, 528, 779
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 415
PaeI GCATGC 1 cut(s) 415
PagI TCATGA 1 cut(s) 802
PceI AGGCCT 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 412, 678
PctI GAATGC 1 cut(s) 638
PfeI GAWTC 2 cut(s) 59, 570
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 4 cut(s) 158, 397, 463, 782
PleI GAGTC 4 cut(s) 144, 262, 378, 623
PpsI GAGTC 4 cut(s) 144, 262, 378, 623
PspN4I GGNNCC 3 cut(s) 249, 528, 779
PspPI GGNCC 1 cut(s) 778
PstI CTGCAG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 754
RsaI GTAC 1 cut(s) 654
RsaNI GTAC 1 cut(s) 653
RseI CAYNNNNRTG 1 cut(s) 516
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 32, 702, 714
SatI GCNGC 4 cut(s) 157, 396, 462, 781
Sau3AI GATC 1 cut(s) 526
Sau96I GGNCC 1 cut(s) 778
SchI GAGTC 4 cut(s) 144, 262, 379, 624
SduI GDGCHC 1 cut(s) 309
SetI ASST 8 cut(s) 130, 301, 349, 384, 463, 483, 720, 756
SfaNI GCATC 3 cut(s) 28, 369, 415
SfcI CTRYAG 2 cut(s) 154, 336
SmiMI CAYNNNNRTG 1 cut(s) 516
SphI GCATGC 1 cut(s) 415
Sse9I AATT 6 cut(s) 64, 209, 385, 585, 706, 771
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 3 cut(s) 5, 727, 781
StuI AGGCCT 1 cut(s) 187
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 5 cut(s) 340, 451, 472, 536, 646
TaqI TCGA 3 cut(s) 57, 221, 227
TasI AATT 6 cut(s) 64, 209, 385, 585, 706, 771
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 2 cut(s) 59, 570
Tru1I TTAA 3 cut(s) 32, 702, 714
Tru9I TTAA 3 cut(s) 32, 702, 714
TscAI CASTG 2 cut(s) 405, 651
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 3 cut(s) 156, 395, 461
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 3 cut(s) 182, 246, 819
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 405, 651
XapI RAATTY 2 cut(s) 585, 706
XceI RCATGY 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.