AT4G38630

26S proteasome non-ATPase regulatory subunit 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001533
Physical Location & Seq
Reverse (-)
18056988 .. 18059598
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38630.1

Sequence Viewer

Length: 1161 bp
ATGGTTCTCGAGGCGACTATGATATGTATCGACAACTCCGAGTGGATGCGAAACGGAGATTACTCTCCGTCTAGGTTACAGGCGCAAACGGAAGCTGTTAATCTTCTTTGCGGAGCCAAAACCCAGTCGAATCCGGAGAATACGGTGGGGATTTTGACAATGGCTGGCAAAGGAGTTAGAGTATTGACTACTCCTACCTCTGATCTTGGCAAAATTCTGGCCTGTATGCACGGCCTTGATGTGGGAGGAGAGATCAACTTAACCGCAGCTATCCAGATCGCCCAGCTAGCTCTTAAGCATCGCCAAAACAAGAATCAACGCCAAAGGATTATTGTTTTTGCTGGAAGTCCAATCAAGTACGAGAAGAAGGCCCTAGAGATAGTTGGAAAAAGGCTGAAGAAGAATAGTGTCTCTCTTGATATTGTCAATTTCGGGGAGGATGATGATGAGGAAAAGCCTCAGAAACTCGAGGCGCTCCTTACAGCTGTGAATAACAATGACGGTAGCCACATTGTTCATGTTCCTTCTGGAGCCAATGCTCTCTCAGATGTGCTTCTCAGCACACCTGTATTCACGGGTGATGAGGGTGCAAGTGGCTATGTTTCTGCGGCAGCTGCTGCAGCGGCCGCAGGTGGGGACTTCGACTTTGGTGTGGACCCAAATATCGATCCAGAACTTGCTCTTGCCCTTCGGGTCTCCATGGAGGAGGAGAGAGCAAGACAAGAAGCTGCTGCCAAGAAGGCGGCCGATGAGGCAGGTCAGAAAGACAAAGATGGGGACACAGCTTCCGCCTCACAGGAGACAGTTGCTAGGACAACTGACAAGAACGCTGAACCAATGGATGAGGACAGTGCGTTGCTAGATCAGGCAATTGCTATGTCTGTTGGTGATGTGAATATGTCAGAAGCGGCTGATGAGGACCAGGATCTGGCTTTAGCTCTGCAAATGTCAATGAGTGGGGAAGAGTCAAGTGAAGCTACAGGTGCTGGAAACAACCTCTTGGGAAATCAAGCCTTCATATCGTCTGTTCTCTCATCGCTTCCTGGGGTGGATCCAAATGATCCGGCAGTTAAAGAACTACTAGCGTCTCTGCCAGACGAGTCAAAGCGTACCGAGGAGGAAGAGAGTAGTAGCAAAAAAGGCGAGGATGAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

386

Amino Acids

40.76

Weight (kDa)

4.47

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 1.2e-31 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 620
Acc36I ACCTGC 2 cut(s) 620, 746
AccB7I CCANNNNNTGG 1 cut(s) 928
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 8 cut(s) 111, 264, 608, 623, 627, 743, 789, 908
AclWI GGATC 5 cut(s) 662, 933, 1046, 1055, 1059
AcoI YGGCCR 2 cut(s) 624, 744
AcsI RAATTY 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 2 cut(s) 359, 1111
AfiI CCNNNNNNNGG 3 cut(s) 241, 633, 928
AflII CTTAAG 1 cut(s) 293
AjnI CCWGG 2 cut(s) 921, 1042
Alw26I GTCTC 4 cut(s) 415, 700, 794, 1092
AlwI GGATC 5 cut(s) 662, 933, 1046, 1055, 1059
AlwNI CAGNNNCTG 3 cut(s) 617, 928, 986
Ama87I CYCGRG 2 cut(s) 8, 467
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 5 cut(s) 219, 232, 369, 624, 744
ApeKI GCWGC 7 cut(s) 266, 611, 614, 617, 620, 728, 731
ApoI RAATTY 1 cut(s) 213
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AspLEI GCGC 2 cut(s) 85, 475
AspS9I GGNCC 3 cut(s) 370, 655, 919
AsuHPI GGTGA 2 cut(s) 590, 899
AsuNHI GCTAGC 1 cut(s) 286
AvaI CYCGRG 2 cut(s) 8, 467
AvaII GGWCC 2 cut(s) 655, 919
BamHI GGATCC 1 cut(s) 1051
BbvI GCAGC 7 cut(s) 278, 601, 604, 623, 632, 715, 718
BccI CCATC 1 cut(s) 767
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 2 cut(s) 923, 1044
BcoDI GTCTC 4 cut(s) 415, 700, 794, 1092
BfaI CTAG 6 cut(s) 72, 287, 374, 810, 860, 1082
BfmI CTRYAG 2 cut(s) 618, 978
BfoI RGCGCY 1 cut(s) 476
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 2 cut(s) 620, 746
BglI GCCNNNNNGGC 2 cut(s) 740, 752
Bme1390I CCNGG 2 cut(s) 923, 1044
Bme18I GGWCC 2 cut(s) 655, 919
BmeT110I CYCGRG 2 cut(s) 8, 467
BmgT120I GGNCC 3 cut(s) 370, 655, 919
BmiI GGNNCC 4 cut(s) 115, 532, 657, 1053
BmrFI CCNGG 2 cut(s) 923, 1044
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 36, 307
BmtI GCTAGC 1 cut(s) 290
BmuI ACTGGG 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 700
BsaJI CCNNGG 3 cut(s) 699, 1043, 1113
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 633, 928
Bse1I ACTGG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 2 cut(s) 923, 1044
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 3 cut(s) 699, 1043, 1113
BseGI GGATG 4 cut(s) 51, 445, 847, 1153
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 241, 633, 928
BseMII CTCAG 3 cut(s) 473, 558, 571
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 4 cut(s) 261, 719, 722, 1130
BseX3I CGGCCG 2 cut(s) 624, 744
BseXI GCAGC 7 cut(s) 278, 601, 604, 623, 632, 715, 718
BseYI CCCAGC 1 cut(s) 282
Bsh1285I CGRYCG 2 cut(s) 627, 747
BshFI GGCC 5 cut(s) 221, 234, 371, 626, 746
BshVI ATCGAT 1 cut(s) 666
BsiEI CGRYCG 2 cut(s) 627, 747
BsiHKCI CYCGRG 2 cut(s) 8, 467
BsiSI CCGG 2 cut(s) 134, 1064
BslFI GGGAC 2 cut(s) 650, 791
BslI CCNNNNNNNGG 3 cut(s) 241, 633, 928
BsmAI GTCTC 4 cut(s) 415, 700, 794, 1092
BsmBI CGTCTC 1 cut(s) 1092
BsmFI GGGAC 2 cut(s) 650, 791
BsnI GGCC 5 cut(s) 221, 234, 371, 626, 746
Bso31I GGTCTC 1 cut(s) 700
BsoBI CYCGRG 2 cut(s) 8, 467
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 8 cut(s) 111, 264, 608, 623, 627, 743, 789, 908
BspANI GGCC 5 cut(s) 221, 234, 371, 626, 746
BspCNI CTCAG 3 cut(s) 472, 557, 570
BspDI ATCGAT 1 cut(s) 666
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 115, 532, 657, 1053
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 2 cut(s) 620, 746
BspOI GCTAGC 1 cut(s) 290
BspPI GGATC 5 cut(s) 662, 933, 1046, 1055, 1059
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 700
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 699, 1043, 1113
BssMI GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 2 cut(s) 923, 1044
Bst4CI ACNGT 4 cut(s) 145, 503, 805, 851
Bst6I CTCTTC 2 cut(s) 957, 1116
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 166, 288
BstDEI CTNAG 3 cut(s) 459, 544, 557
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 4 cut(s) 51, 445, 847, 1153
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 2 cut(s) 85, 475
BstKTI GATC 8 cut(s) 205, 255, 279, 670, 865, 928, 1054, 1063
BstMAI GTCTC 4 cut(s) 415, 700, 794, 1092
BstMBI GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
BstMCI CGRYCG 2 cut(s) 627, 747
BstNI CCWGG 2 cut(s) 923, 1044
BstSCI CCNGG 2 cut(s) 921, 1042
BstSFI CTRYAG 2 cut(s) 618, 978
BstV1I GCAGC 7 cut(s) 278, 601, 604, 623, 632, 715, 718
BstX2I RGATCY 2 cut(s) 925, 1051
BstYI RGATCY 2 cut(s) 925, 1051
BstZI CGGCCG 2 cut(s) 624, 744
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 5 cut(s) 221, 234, 371, 626, 746
BsuTUI ATCGAT 1 cut(s) 666
BtgI CCRYGG 1 cut(s) 699
BtgZI GCGATG 2 cut(s) 284, 1020
BtsCI GGATG 4 cut(s) 51, 445, 847, 1153
BtsIMutI CAGTG 1 cut(s) 856
BveI ACCTGC 2 cut(s) 620, 746
Cac8I GCNNGC 2 cut(s) 166, 288
CaiI CAGNNNCTG 3 cut(s) 617, 928, 986
CciNI GCGGCCGC 1 cut(s) 624
CfoI GCGC 2 cut(s) 85, 475
Cfr13I GGNCC 3 cut(s) 370, 655, 919
ClaI ATCGAT 1 cut(s) 666
CseI GACGC 1 cut(s) 1074
Csp6I GTAC 2 cut(s) 358, 1110
CviAII CATG 2 cut(s) 518, 700
CviQI GTAC 2 cut(s) 358, 1110
DdeI CTNAG 3 cut(s) 459, 544, 557
DpnI GATC 8 cut(s) 204, 254, 278, 669, 864, 927, 1053, 1062
DpnII GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
EaeI YGGCCR 2 cut(s) 624, 744
EagI CGGCCG 2 cut(s) 624, 744
Eam1104I CTCTTC 2 cut(s) 957, 1116
EarI CTCTTC 2 cut(s) 957, 1116
EciI GGCGGA 1 cut(s) 778
EclXI CGGCCG 2 cut(s) 624, 744
Eco130I CCWWGG 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 700
Eco47I GGWCC 2 cut(s) 655, 919
Eco52I CGGCCG 2 cut(s) 624, 744
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 2 cut(s) 8, 467
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 921, 1042
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 1092
FaeI CATG 2 cut(s) 521, 703
FaiI YATR 9 cut(s) 20, 25, 227, 519, 600, 701, 878, 899, 1019
FaqI GGGAC 2 cut(s) 650, 791
FatI CATG 2 cut(s) 517, 699
FokI GGATG 3 cut(s) 58, 452, 854
FspBI CTAG 6 cut(s) 72, 287, 374, 810, 860, 1082
GlaI GCGC 2 cut(s) 84, 474
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 5 cut(s) 221, 234, 371, 626, 746
HapII CCGG 2 cut(s) 134, 1064
HgaI GACGC 1 cut(s) 1074
HhaI GCGC 2 cut(s) 85, 475
Hin1II CATG 2 cut(s) 521, 703
Hin6I GCGC 2 cut(s) 83, 473
HinP1I GCGC 2 cut(s) 83, 473
HinfI GANTC 4 cut(s) 130, 313, 965, 1100
HpaII CCGG 2 cut(s) 134, 1064
HphI GGTGA 2 cut(s) 590, 899
Hpy166II GTNNAC 1 cut(s) 655
Hpy188I TCNGA 6 cut(s) 40, 202, 462, 547, 762, 904
Hpy188III TCNNGA 6 cut(s) 8, 134, 274, 416, 528, 671
Hpy8I GTNNAC 1 cut(s) 655
HpyAV CCTTC 5 cut(s) 361, 534, 698, 733, 1024
HpyCH4III ACNGT 4 cut(s) 145, 503, 805, 851
HpyCH4V TGCA 4 cut(s) 229, 590, 620, 943
HpyF3I CTNAG 3 cut(s) 459, 544, 557
Hsp92II CATG 2 cut(s) 521, 703
HspAI GCGC 2 cut(s) 83, 473
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
LmnI GCTCC 3 cut(s) 113, 480, 530
Lsp1109I GCAGC 7 cut(s) 278, 601, 604, 623, 632, 715, 718
LweI GCATC 2 cut(s) 36, 307
MaeI CTAG 6 cut(s) 72, 287, 374, 810, 860, 1082
MaeIII GTNAC 1 cut(s) 75
MalI GATC 8 cut(s) 204, 254, 278, 669, 864, 927, 1053, 1062
MboI GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
MboII GAAGA 6 cut(s) 95, 376, 409, 412, 974, 1133
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 2 cut(s) 925, 1051
MluCI AATT 3 cut(s) 213, 427, 870
MlyI GAGTC 2 cut(s) 974, 1109
MmeI TCCRAC 1 cut(s) 364
MroI TCCGGA 1 cut(s) 133
MseI TTAA 4 cut(s) 99, 260, 294, 1071
MspA1I CMGCKG 3 cut(s) 485, 614, 623
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 2 cut(s) 134, 1064
MspR9I CCNGG 2 cut(s) 923, 1044
MunI CAATTG 1 cut(s) 870
MvaI CCWGG 2 cut(s) 923, 1044
NcoI CCATGG 1 cut(s) 699
NdeII GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
NheI GCTAGC 1 cut(s) 286
NlaIII CATG 2 cut(s) 521, 703
NlaIV GGNNCC 4 cut(s) 115, 532, 657, 1053
NotI GCGGCCGC 1 cut(s) 624
PaeR7I CTCGAG 2 cut(s) 8, 467
PaqCI CACCTGC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 2 cut(s) 130, 313
PflMI CCANNNNNTGG 1 cut(s) 928
PleI GAGTC 2 cut(s) 973, 1108
PpsI GAGTC 2 cut(s) 973, 1108
Psp6I CCWGG 2 cut(s) 921, 1042
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 2 cut(s) 921, 1042
PspN4I GGNNCC 4 cut(s) 115, 532, 657, 1053
PspPI GGNCC 3 cut(s) 370, 655, 919
PspXI VCTCGAGB 1 cut(s) 467
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 3 cut(s) 617, 928, 986
PsuI RGATCY 2 cut(s) 925, 1051
PvuII CAGCTG 2 cut(s) 485, 614
RsaI GTAC 2 cut(s) 359, 1111
RsaNI GTAC 2 cut(s) 358, 1110
SaqAI TTAA 4 cut(s) 99, 260, 294, 1071
Sau3AI GATC 8 cut(s) 202, 252, 276, 667, 862, 925, 1051, 1060
Sau96I GGNCC 3 cut(s) 370, 655, 919
SchI GAGTC 2 cut(s) 974, 1109
ScrFI CCNGG 2 cut(s) 923, 1044
SfaNI GCATC 2 cut(s) 36, 307
SfcI CTRYAG 2 cut(s) 618, 978
Sfr274I CTCGAG 2 cut(s) 8, 467
SinI GGWCC 2 cut(s) 655, 919
SlaI CTCGAG 2 cut(s) 8, 467
SmlI CTYRAG 3 cut(s) 8, 293, 467
SmoI CTYRAG 3 cut(s) 8, 293, 467
Sse9I AATT 3 cut(s) 213, 427, 870
SsiI CCGC 8 cut(s) 111, 264, 608, 623, 627, 743, 789, 908
SspMI CTAG 6 cut(s) 72, 287, 374, 810, 860, 1082
StyD4I CCNGG 2 cut(s) 921, 1042
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 4 cut(s) 145, 503, 805, 851
TaqI TCGA 6 cut(s) 9, 30, 128, 468, 642, 666
TasI AATT 3 cut(s) 213, 427, 870
TauI GCSGC 5 cut(s) 611, 626, 629, 746, 911
TfiI GAWTC 2 cut(s) 130, 313
Tru1I TTAA 4 cut(s) 99, 260, 294, 1071
Tru9I TTAA 4 cut(s) 99, 260, 294, 1071
TscAI CASTG 1 cut(s) 856
TseI GCWGC 7 cut(s) 266, 611, 614, 617, 620, 728, 731
TspDTI ATGAA 2 cut(s) 506, 1006
TspGWI ACGGA 3 cut(s) 57, 69, 104
TspRI CASTG 1 cut(s) 856
Van91I CCANNNNNTGG 1 cut(s) 928
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 655, 919
XapI RAATTY 1 cut(s) 213
XhoI CTCGAG 2 cut(s) 8, 467
XspI CTAG 6 cut(s) 72, 287, 374, 810, 860, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.