MD02G1129100.v1.1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001533
Physical Location & Seq
Reverse (-)
10442878 .. 10447664
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1129100.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGCAAGTCCTTTTGGGCCGGGCCGAGGACTCAAGCTTACGTGGCGACGAGTCACTGGTTGAATTCTCGGTACAGTTTTCACTCAAAAGTCCTGGTCCGCACCTGATAATTAGAACGCACAGGAAGGCAGCCTCCTCTGCAAGTAAAGTAGTCCTCAATCCTCTACTTGTATGTTCTTTTCGATCAAACAATCTCGATCTTTCTTCACAATCTGCAACCATGGTGCTCGAGGCGACAATGATCTGCATTGATAATTCGGAATGGATGCGGAACGGCGATTATGCTCCGTCGAGGTTTCAAGCTCAGGCCGATGCTGTCAATCTGATCTGCGGAGCTAAAACCCATTCTAATCCGGAGAATACAGTAGGAGTTCTCACCATGGCAGGAAAAGGTGTTCGTGTTTTGGTCACTCCAACCAGTGATCTTGGCAAGATTCTGGCATGCATGCATGGTTTAGAAATTGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCTCTCAAGCATCGCCAAAATAAGAAACAGCAACAGAGGATTATTGTGTTTGCTGGAAGTCCTGTTAAACATGAGAAAAAGCTTTTAGAGATGATTGGAAGGAAGTTAAAGAAGAACAGTGTAGCCCTCGATATCGTCAATTTTGGTGAAGAAGATGAAGATAAGTCAGAGAAGCTAGAAGCACTACTTGCTGCTGTGAACAATAATGACACCAGTCACATTGTTCATGTACCAGCAGGTCCAAGTGCTCTTTCTGATGTACTTATAAGCACACCCATCTTCACTGGGGATGGGGAAGGTGGGAGTGGTTTTGCAGCGGCAGCTGCTGCTGCTGCTGCAGGTGGTGTCTCTGGTTTTGATTTCGGCGTGGATCCAAACCTGGATCCTGAGCTTGCCCTTGCTCTTAGAGTTTCAATGGAAGAGGAGAGGGCCAGACAAGAAGCAGCTGCTAAAAAAGCAGCTGAGGATGCTGGCAAACATGAAAAAGGAGGGGAGGGGCAATCCAGCTCACAAGATGCAACGATGACGGAGCGTGCCAGTGTTGGGACTTCTGAAGCTGAGAATAAGAGTAGTGATTTCATGGATGATGAGAATGCTCTGCTACAACAGGCCCTTGCAATGTCGATGGATGAACCTGCTTCCAGCCATGATATTCGAGATACGGATATGTCTGAGGCAACTGACCCAGAGTTGGCTCTAGCTCTTCAATTGTCTGTTCAGGAAGGTGAAAAAGATTCTGATAGCGAGAAAGATATGAGTAAATTATTAGCCGACCAGTCTTTCGTGTCTTCCATCCTTGCCTCGCTTCCAGGGGTTGACCCAAATGATCCTTCAGTTAAAGATTTGCTTGCTTCCATGCAAAACCCGTCAGAGCCTGATCAGAAGAACGAAGACAAACCACCAAAAGAGGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

476

Amino Acids

50.53

Weight (kDa)

4.76

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 79 - 188 2.8e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AarI CACCTGC 1 cut(s) 836
Acc36I ACCTGC 3 cut(s) 734, 836, 1150
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 4 cut(s) 98, 268, 330, 824
AclWI GGATC 5 cut(s) 872, 884, 885, 897, 1328
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 2 cut(s) 1080, 1323
AfaI GTAC 3 cut(s) 72, 738, 768
AfiI CCNNNNNNNGG 3 cut(s) 25, 1050, 1198
AgsI TTSAA 4 cut(s) 62, 299, 921, 1214
AjnI CCWGG 4 cut(s) 91, 477, 885, 1315
AjuI GAANNNNNNNTTGG 4 cut(s) 742, 774, 1321, 1353
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
Alw21I GWGCWC 2 cut(s) 228, 757
Alw26I GTCTC 1 cut(s) 859
AlwI GGATC 5 cut(s) 872, 884, 885, 897, 1328
AlwNI CAGNNNCTG 2 cut(s) 833, 1382
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 5 cut(s) 16, 21, 306, 936, 1116
ApoI RAATTY 1 cut(s) 62
ArsI GACNNNNNNTTYG 2 cut(s) 1271, 1303
AspS9I GGNCC 6 cut(s) 16, 21, 95, 746, 936, 1117
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 4 cut(s) 367, 479, 665, 1244
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 2 cut(s) 95, 746
BamHI GGATCC 2 cut(s) 877, 889
BbsI GAAGAC 2 cut(s) 1287, 1404
Bbv12I GWGCWC 2 cut(s) 228, 757
BbvCI CCTCAGC 1 cut(s) 969
BccI CCATC 4 cut(s) 791, 791, 1126, 1307
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 4 cut(s) 93, 479, 887, 1317
BclI TGATCA 1 cut(s) 1384
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 1 cut(s) 859
BfaI CTAG 2 cut(s) 683, 1205
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 3 cut(s) 734, 836, 1150
Bme1390I CCNGG 5 cut(s) 20, 93, 479, 887, 1317
Bme18I GGWCC 2 cut(s) 95, 746
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 6 cut(s) 16, 21, 95, 746, 936, 1117
BmiI GGNNCC 2 cut(s) 879, 891
BmrFI CCNGG 5 cut(s) 20, 93, 479, 887, 1317
BmrI ACTGGG 1 cut(s) 801
BmsI GCATC 5 cut(s) 255, 301, 526, 964, 1012
BmuI ACTGGG 1 cut(s) 801
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 2 cut(s) 1287, 1404
Bpu10I CCTNAGC 3 cut(s) 303, 894, 969
BpuEI CTTGAG 2 cut(s) 16, 497
BpuMI CCSGG 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 24, 219, 378, 1316
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 1050, 1198
Bse1I ACTGG 6 cut(s) 60, 417, 720, 796, 1044, 1282
Bse3DI GCAATG 1 cut(s) 1131
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 4 cut(s) 93, 479, 887, 1317
BseDI CCNNGG 4 cut(s) 24, 219, 378, 1316
BseGI GGATG 6 cut(s) 270, 802, 979, 1096, 1141, 1299
BseLI CCNNNNNNNGG 3 cut(s) 25, 1050, 1198
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 5 cut(s) 317, 885, 960, 1056, 1170
BseNI ACTGG 6 cut(s) 60, 417, 720, 796, 1044, 1282
BseRI GAGGAG 2 cut(s) 124, 944
BshFI GGCC 5 cut(s) 18, 23, 308, 938, 1118
BsiHKAI GWGCWC 2 cut(s) 228, 757
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 2 cut(s) 19, 353
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 3 cut(s) 25, 1050, 1198
BsmAI GTCTC 1 cut(s) 859
BsmFI GGGAC 1 cut(s) 1066
BsmI GAATGC 2 cut(s) 489, 1105
BsnI GGCC 5 cut(s) 18, 23, 308, 938, 1118
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 228, 757
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
Bsp19I CCATGG 2 cut(s) 219, 378
BspACI CCGC 4 cut(s) 98, 268, 330, 824
BspANI GGCC 5 cut(s) 18, 23, 308, 938, 1118
BspCNI CTCAG 5 cut(s) 316, 886, 961, 1057, 1171
BspEI TCCGGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 879, 891
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 3 cut(s) 734, 836, 1150
BspPI GGATC 5 cut(s) 872, 884, 885, 897, 1328
BspQI GCTCTTC 1 cut(s) 1215
BsrDI GCAATG 1 cut(s) 1131
BsrI ACTGG 6 cut(s) 60, 417, 720, 796, 1044, 1282
BssECI CCNNGG 4 cut(s) 24, 219, 378, 1316
BssMI GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
BssT1I CCWWGG 2 cut(s) 219, 378
Bst2UI CCWGG 4 cut(s) 93, 479, 887, 1317
Bst4CI ACNGT 3 cut(s) 75, 364, 626
Bst6I CTCTTC 2 cut(s) 921, 1215
BstAPI GCANNNNNTGC 2 cut(s) 695, 833
BstBAI YACGTR 1 cut(s) 41
BstC8I GCNNGC 7 cut(s) 442, 446, 487, 900, 979, 1041, 1356
BstDEI CTNAG 6 cut(s) 303, 894, 911, 969, 1065, 1179
BstDSI CCRYGG 2 cut(s) 219, 378
BstF5I GGATG 6 cut(s) 270, 802, 979, 1096, 1141, 1299
BstKTI GATC 9 cut(s) 185, 199, 243, 327, 424, 880, 892, 1336, 1387
BstMAI GTCTC 1 cut(s) 859
BstMBI GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
BstNI CCWGG 4 cut(s) 93, 479, 887, 1317
BstNSI RCATGY 2 cut(s) 444, 448
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 5 cut(s) 18, 91, 477, 885, 1315
BstSFI CTRYAG 1 cut(s) 843
BstV2I GAAGAC 2 cut(s) 1287, 1404
BstX2I RGATCY 2 cut(s) 877, 889
BstYI RGATCY 2 cut(s) 877, 889
BsuRI GGCC 5 cut(s) 18, 23, 308, 938, 1118
BtgI CCRYGG 2 cut(s) 219, 378
BtgZI GCGATG 1 cut(s) 503
BtsCI GGATG 6 cut(s) 270, 802, 979, 1096, 1141, 1299
BtsIMutI CAGTG 5 cut(s) 53, 424, 631, 789, 1051
BveI ACCTGC 3 cut(s) 734, 836, 1150
Cac8I GCNNGC 7 cut(s) 442, 446, 487, 900, 979, 1041, 1356
CaiI CAGNNNCTG 2 cut(s) 833, 1382
Cfr13I GGNCC 6 cut(s) 16, 21, 95, 746, 936, 1117
Csp6I GTAC 3 cut(s) 71, 737, 767
CviQI GTAC 3 cut(s) 71, 737, 767
DdeI CTNAG 6 cut(s) 303, 894, 911, 969, 1065, 1179
DpnI GATC 9 cut(s) 184, 198, 242, 326, 423, 879, 891, 1335, 1386
DpnII GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
Eam1104I CTCTTC 2 cut(s) 921, 1215
EarI CTCTTC 2 cut(s) 921, 1215
Eco130I CCWWGG 2 cut(s) 219, 378
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 95, 746
Eco57I CTGAAG 2 cut(s) 1080, 1323
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 1117
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 4 cut(s) 91, 477, 885, 1315
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 219, 378
EcoT22I ATGCAT 2 cut(s) 446, 450
ErhI CCWWGG 2 cut(s) 219, 378
FalI AAGNNNNNCTT 4 cut(s) 678, 710, 1338, 1370
FaqI GGGAC 1 cut(s) 1066
FbaI TGATCA 1 cut(s) 1384
FokI GGATG 6 cut(s) 277, 809, 986, 1103, 1148, 1286
FspBI CTAG 2 cut(s) 683, 1205
HaeIII GGCC 5 cut(s) 18, 23, 308, 938, 1118
HapII CCGG 2 cut(s) 19, 353
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HindIII AAGCTT 2 cut(s) 34, 587
HinfI GANTC 4 cut(s) 29, 50, 433, 1241
HpaII CCGG 2 cut(s) 19, 353
HphI GGTGA 4 cut(s) 367, 479, 665, 1244
Hpy166II GTNNAC 2 cut(s) 706, 1324
Hpy188I TCNGA 9 cut(s) 259, 324, 676, 763, 1060, 1180, 1246, 1378, 1389
Hpy188III TCNNGA 5 cut(s) 194, 353, 893, 1163, 1226
Hpy8I GTNNAC 2 cut(s) 706, 1324
Hpy99I CGWCG 2 cut(s) 50, 292
HpyAV CCTTC 5 cut(s) 118, 600, 797, 1223, 1347
HpyCH4III ACNGT 3 cut(s) 75, 364, 626
HpyCH4IV ACGT 1 cut(s) 40
HpyF3I CTNAG 6 cut(s) 303, 894, 911, 969, 1065, 1179
HpySE526I ACGT 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 1384
Kzo9I GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
LguI GCTCTTC 1 cut(s) 1215
LmnI GCTCC 3 cut(s) 289, 332, 1036
LweI GCATC 5 cut(s) 255, 301, 526, 964, 1012
MaeI CTAG 2 cut(s) 683, 1205
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 3 cut(s) 51, 406, 722
MalI GATC 9 cut(s) 184, 198, 242, 326, 423, 879, 891, 1335, 1386
MboI GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
MfeI CAATTG 2 cut(s) 503, 1214
MflI RGATCY 2 cut(s) 877, 889
MhlI GDGCHC 2 cut(s) 228, 757
MluCI AATT 8 cut(s) 62, 108, 253, 459, 503, 646, 1214, 1268
MlyI GAGTC 2 cut(s) 23, 59
MmeI TCCRAC 1 cut(s) 437
Mph1103I ATGCAT 2 cut(s) 446, 450
MroI TCCGGA 1 cut(s) 352
MseI TTAA 3 cut(s) 573, 614, 1344
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 4 cut(s) 824, 830, 953, 968
MspI CCGG 2 cut(s) 19, 353
MspR9I CCNGG 5 cut(s) 20, 93, 479, 887, 1317
MunI CAATTG 2 cut(s) 503, 1214
Mva1269I GAATGC 2 cut(s) 489, 1105
MvaI CCWGG 4 cut(s) 93, 479, 887, 1317
NciI CCSGG 1 cut(s) 20
NcoI CCATGG 2 cut(s) 219, 378
NdeII GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
NlaIV GGNNCC 2 cut(s) 879, 891
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 3 cut(s) 51, 406, 722
NsiI ATGCAT 2 cut(s) 446, 450
NspI RCATGY 2 cut(s) 444, 448
PaeI GCATGC 2 cut(s) 444, 448
PaeR7I CTCGAG 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 1215
PctI GAATGC 2 cut(s) 489, 1105
PfeI GAWTC 2 cut(s) 433, 1241
PleI GAGTC 2 cut(s) 23, 58
PpsI GAGTC 2 cut(s) 23, 58
Ppu21I YACGTR 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 720
PsiI TTATAA 1 cut(s) 773
Psp6I CCWGG 4 cut(s) 91, 477, 885, 1315
PspGI CCWGG 4 cut(s) 91, 477, 885, 1315
PspN4I GGNNCC 2 cut(s) 879, 891
PspPI GGNCC 6 cut(s) 16, 21, 95, 746, 936, 1117
PspXI VCTCGAGB 1 cut(s) 227
PstI CTGCAG 1 cut(s) 847
PstNI CAGNNNCTG 2 cut(s) 833, 1382
PsuI RGATCY 2 cut(s) 877, 889
PvuII CAGCTG 3 cut(s) 830, 953, 968
RsaI GTAC 3 cut(s) 72, 738, 768
RsaNI GTAC 3 cut(s) 71, 737, 767
RseI CAYNNNNRTG 1 cut(s) 494
SapI GCTCTTC 1 cut(s) 1215
SaqAI TTAA 3 cut(s) 573, 614, 1344
Sau3AI GATC 9 cut(s) 182, 196, 240, 324, 421, 877, 889, 1333, 1384
Sau96I GGNCC 6 cut(s) 16, 21, 95, 746, 936, 1117
SchI GAGTC 2 cut(s) 23, 59
ScrFI CCNGG 5 cut(s) 20, 93, 479, 887, 1317
SduI GDGCHC 2 cut(s) 228, 757
SfaNI GCATC 5 cut(s) 255, 301, 526, 964, 1012
SfcI CTRYAG 1 cut(s) 843
Sfr274I CTCGAG 1 cut(s) 227
SinI GGWCC 2 cut(s) 95, 746
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 3 cut(s) 31, 227, 512
SmoI CTYRAG 3 cut(s) 31, 227, 512
SphI GCATGC 2 cut(s) 444, 448
Sse9I AATT 8 cut(s) 62, 108, 253, 459, 503, 646, 1214, 1268
SsiI CCGC 4 cut(s) 98, 268, 330, 824
SspMI CTAG 2 cut(s) 683, 1205
StyD4I CCNGG 5 cut(s) 18, 91, 477, 885, 1315
StyI CCWWGG 2 cut(s) 219, 378
TaaI ACNGT 3 cut(s) 75, 364, 626
TaiI ACGT 1 cut(s) 43
TaqI TCGA 7 cut(s) 181, 195, 228, 290, 636, 1130, 1162
TasI AATT 8 cut(s) 62, 108, 253, 459, 503, 646, 1214, 1268
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 827
TfiI GAWTC 2 cut(s) 433, 1241
Tru1I TTAA 3 cut(s) 573, 614, 1344
Tru9I TTAA 3 cut(s) 573, 614, 1344
TscAI CASTG 5 cut(s) 60, 424, 631, 796, 1051
TseFI GTSAC 3 cut(s) 51, 406, 722
Tsp45I GTSAC 3 cut(s) 51, 406, 722
TspDTI ATGAA 6 cut(s) 488, 678, 722, 1002, 1075, 1152
TspGWI ACGGA 3 cut(s) 276, 1049, 1184
TspRI CASTG 5 cut(s) 60, 424, 631, 796, 1051
VpaK11BI GGWCC 2 cut(s) 95, 746
XapI RAATTY 1 cut(s) 62
XceI RCATGY 2 cut(s) 444, 448
XhoI CTCGAG 1 cut(s) 227
XspI CTAG 2 cut(s) 683, 1205
Zsp2I ATGCAT 2 cut(s) 446, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.