FvH4_1g11620

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001533
Physical Location & Seq
Reverse (-)
6348793 .. 6354017
5225 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11620.t4

Sequence Viewer

Length: 1215 bp
ATGGTGCTCGAGGCGACGATGATCTGCATTGATAATTCGGAGTGGATGCGTAACGGCGATTACGCTCCGACGAGATTTCAAGCTCAGGCCGACGCCGTCAATCTGATTTGCGGAGCCAAAACCCAGTCTAATCCGGAGAATACAGTGGGAGTTCTCACCATGGCTGGGAAAGGTGTTCGCGTTTTGGTTACTCCTACTAGTGATCTTGGCAAGATTTTGGCTTGCATGCACGGTTTAGAAATAGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCCCTCAAACATCGCCAGAATAAGAATCAGCAACAAAGGATTATAGTCTTTGCTGGAAGTGCCGTTAATCATGAAAAGAAGACTTTGGAGATGATTGGCAGAAAGTTAAAGAAGAACAGTGTAGCCCTTGATATCATCAATTTTGGTGAAGAAGATGAAGAAAAGAAAGAGAAGCTAGAAGCACTTCTTGCTGCTGTGAACAATAATAACAGCAACATTGTTCATGTTCAGGCTGGTCCAAGTGCTCTTTCGGATGTACTCATAAGTACACCTATCTTCACCGGAGATGGAGAAGGTGGTAGTGGTTTTGCAGCAGCAGCAGCTGCTGCTGCAGCCGGAGGTGTCTCTGGTTTTGATTTTGGTGTGGATCCAAACCTGGATCCTGAGCTTGCCCTTGCTCTTAGAGTATCAATGGAGGAGGAGAGGGCCAGACAGGAAGCAGCAGCTAAAAAGGCTGCTGAGGATTCTTCCAAACAGGAAAAAGGAGGGGAAGAACCATCCAGCTCACAAGATGCAACTATGACAGAGCGTGCCAGTGCTGTAACTTCTGAAGCTGAGAATAAGAGTATTGATTTCATGGATGATGACAACGCTCTGCTGCAGCAGGCCCTTGCGATGTCGATGGATGAACCTGCCTCCAGCCATGATTTGCGAGACACTGACATGTCTGAGGCAGTTGCAGATGATCCTGAGTTGGCTCTAGCTCTTCAAATGTCTGTGCAGGAAGGTGAGAAAGATTCTGCTAGCGATAAAGATATGAGCAAATTGTTGGCTGATCAGTCTTTTGTGTCTTCTATCCTTGCCTCACTTCCAGGGGTTGACCCAAATGATCCTTCAGTAAGAGACTTGCTTGCTTCTATGCAAAACCAGTCAGAGCCTGAACAAAAGAAGAATGAAGAGAACAAGCCACCAAATGATGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

405

Amino Acids

42.98

Weight (kDa)

4.5

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 6.3e-33 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 928
AccII CGCG 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 111
AclWI GGATC 6 cut(s) 650, 662, 663, 675, 968, 1112
AcuI CTGAAG 2 cut(s) 858, 1107
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 546, 556
AfiI CCNNNNNNNGG 1 cut(s) 165
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 2 cut(s) 80, 998
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 3 cut(s) 258, 663, 1099
AjuI GAANNNNNNNTTGG 4 cut(s) 520, 552, 1105, 1137
AluBI AGCT 8 cut(s) 83, 463, 611, 676, 734, 792, 842, 992
AluI AGCT 8 cut(s) 83, 463, 611, 676, 734, 792, 842, 992
Alw21I GWGCWC 2 cut(s) 9, 535
Alw26I GTCTC 3 cut(s) 637, 936, 1125
AlwI GGATC 6 cut(s) 650, 662, 663, 675, 968, 1112
AlwNI CAGNNNCTG 3 cut(s) 611, 614, 1166
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 4 cut(s) 87, 288, 714, 894
Asp700I GAANNNNTTC 1 cut(s) 471
AspS9I GGNCC 4 cut(s) 289, 524, 714, 895
AsuHPI GGTGA 5 cut(s) 148, 260, 446, 559, 1028
AsuNHI GCTAGC 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 524
BamHI GGATCC 2 cut(s) 655, 667
BbsI GAAGAC 2 cut(s) 374, 1071
Bbv12I GWGCWC 2 cut(s) 9, 535
BbvCI CCTCAGC 1 cut(s) 747
BccI CCATC 3 cut(s) 569, 793, 904
BceAI ACGGC 3 cut(s) 70, 80, 335
BciT130I CCWGG 3 cut(s) 260, 665, 1101
BclI TGATCA 1 cut(s) 1063
BcoDI GTCTC 3 cut(s) 637, 936, 1125
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 4 cut(s) 198, 464, 989, 1032
BfmI CTRYAG 2 cut(s) 618, 887
BfuAI ACCTGC 1 cut(s) 928
Bme1390I CCNGG 3 cut(s) 260, 665, 1101
Bme18I GGWCC 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 4 cut(s) 289, 524, 714, 895
BmiI GGNNCC 3 cut(s) 115, 657, 669
BmrFI CCNGG 3 cut(s) 260, 665, 1101
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 36, 790
BmtI GCTAGC 1 cut(s) 1035
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 2 cut(s) 374, 1071
BpmI CTGGAG 1 cut(s) 910
Bpu10I CCTNAGC 3 cut(s) 84, 672, 747
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 159, 1100
BsaWI WCCGGW 2 cut(s) 133, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse1I ACTGG 3 cut(s) 124, 822, 1156
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 3 cut(s) 260, 665, 1101
BseDI CCNNGG 2 cut(s) 159, 1100
BseGI GGATG 5 cut(s) 51, 547, 785, 874, 919
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMII CTCAG 6 cut(s) 98, 663, 738, 834, 948, 969
BseNI ACTGG 3 cut(s) 124, 822, 1156
BseRI GAGGAG 2 cut(s) 719, 722
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 1028
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 89, 290, 716, 896
BsiHKAI GWGCWC 2 cut(s) 9, 535
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 3 cut(s) 134, 570, 624
BslI CCNNNNNNNGG 1 cut(s) 165
BsmAI GTCTC 3 cut(s) 637, 936, 1125
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 4 cut(s) 89, 290, 716, 896
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 9, 535
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 1 cut(s) 111
BspANI GGCC 4 cut(s) 89, 290, 716, 896
BspCNI CTCAG 6 cut(s) 97, 664, 739, 835, 949, 970
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 115, 657, 669
BspMAI CTGCAG 2 cut(s) 622, 891
BspMI ACCTGC 1 cut(s) 928
BspOI GCTAGC 1 cut(s) 1035
BspPI GGATC 6 cut(s) 650, 662, 663, 675, 968, 1112
BspQI GCTCTTC 1 cut(s) 999
BsrI ACTGG 3 cut(s) 124, 822, 1156
BssECI CCNNGG 2 cut(s) 159, 1100
BssMI GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 3 cut(s) 260, 665, 1101
Bst4CI ACNGT 3 cut(s) 145, 233, 407
Bst6I CTCTTC 2 cut(s) 999, 1179
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 3 cut(s) 476, 611, 614
BstC8I GCNNGC 8 cut(s) 223, 227, 268, 678, 819, 894, 1033, 1140
BstDEI CTNAG 7 cut(s) 84, 672, 689, 747, 843, 957, 978
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 5 cut(s) 51, 547, 785, 874, 919
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 7 cut(s) 24, 205, 658, 670, 976, 1066, 1120
BstMAI GTCTC 3 cut(s) 637, 936, 1125
BstMBI GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
BstNI CCWGG 3 cut(s) 260, 665, 1101
BstNSI RCATGY 2 cut(s) 229, 955
BstSCI CCNGG 3 cut(s) 258, 663, 1099
BstSFI CTRYAG 2 cut(s) 618, 887
BstUI CGCG 1 cut(s) 180
BstV2I GAAGAC 2 cut(s) 374, 1071
BstX2I RGATCY 2 cut(s) 655, 667
BstYI RGATCY 2 cut(s) 655, 667
BsuRI GGCC 4 cut(s) 89, 290, 716, 896
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 2 cut(s) 284, 917
BtsCI GGATG 5 cut(s) 51, 547, 785, 874, 919
BtsIMutI CAGTG 4 cut(s) 150, 412, 829, 945
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 8 cut(s) 223, 227, 268, 678, 819, 894, 1033, 1140
CaiI CAGNNNCTG 3 cut(s) 611, 614, 1166
CciI TCATGA 1 cut(s) 358
Cfr13I GGNCC 4 cut(s) 289, 524, 714, 895
CseI GACGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 545, 555
CviAII CATG 7 cut(s) 160, 226, 359, 512, 865, 932, 952
CviQI GTAC 2 cut(s) 545, 555
DdeI CTNAG 7 cut(s) 84, 672, 689, 747, 843, 957, 978
DpnI GATC 7 cut(s) 23, 204, 657, 669, 975, 1065, 1119
DpnII GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
Eam1104I CTCTTC 2 cut(s) 999, 1179
EarI CTCTTC 2 cut(s) 999, 1179
Eco130I CCWWGG 1 cut(s) 159
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 524
Eco57I CTGAAG 2 cut(s) 858, 1107
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 895
EcoRII CCWGG 3 cut(s) 258, 663, 1099
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 7 cut(s) 163, 229, 362, 515, 868, 935, 955
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FatI CATG 7 cut(s) 159, 225, 358, 511, 864, 931, 951
FbaI TGATCA 1 cut(s) 1063
FokI GGATG 5 cut(s) 58, 554, 772, 881, 926
FspBI CTAG 4 cut(s) 198, 464, 989, 1032
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 910
HaeIII GGCC 4 cut(s) 89, 290, 716, 896
HapII CCGG 3 cut(s) 134, 570, 624
HgaI GACGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 7 cut(s) 163, 229, 362, 515, 868, 935, 955
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HinfI GANTC 3 cut(s) 313, 752, 1025
HpaII CCGG 3 cut(s) 134, 570, 624
HphI GGTGA 5 cut(s) 148, 260, 446, 559, 1028
Hpy166II GTNNAC 3 cut(s) 487, 557, 1108
Hpy188I TCNGA 7 cut(s) 40, 69, 105, 541, 838, 958, 1162
Hpy188III TCNNGA 4 cut(s) 134, 359, 671, 977
Hpy8I GTNNAC 3 cut(s) 487, 557, 1108
Hpy99I CGWCG 3 cut(s) 19, 73, 95
HpyAV CCTTC 3 cut(s) 575, 1007, 1131
HpyCH4III ACNGT 3 cut(s) 145, 233, 407
HpyF3I CTNAG 7 cut(s) 84, 672, 689, 747, 843, 957, 978
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 7 cut(s) 163, 229, 362, 515, 868, 935, 955
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 1063
Kzo9I GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
LguI GCTCTTC 1 cut(s) 999
LmnI GCTCC 2 cut(s) 70, 113
LweI GCATC 2 cut(s) 36, 790
MaeI CTAG 4 cut(s) 198, 464, 989, 1032
MaeIII GTNAC 3 cut(s) 50, 187, 829
MalI GATC 7 cut(s) 23, 204, 657, 669, 975, 1065, 1119
MboI GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 2 cut(s) 655, 667
MhlI GDGCHC 2 cut(s) 9, 535
MluCI AATT 4 cut(s) 34, 284, 427, 1052
MmeI TCCRAC 1 cut(s) 92
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 2 cut(s) 354, 395
MslI CAYNNNNRTG 2 cut(s) 275, 950
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 3 cut(s) 134, 570, 624
MspR9I CCNGG 3 cut(s) 260, 665, 1101
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 270
MvaI CCWGG 3 cut(s) 260, 665, 1101
MvnI CGCG 1 cut(s) 180
NcoI CCATGG 1 cut(s) 159
NdeII GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 7 cut(s) 163, 229, 362, 515, 868, 935, 955
NlaIV GGNNCC 3 cut(s) 115, 657, 669
NspI RCATGY 2 cut(s) 229, 955
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 8
PagI TCATGA 1 cut(s) 358
PciI ACATGT 1 cut(s) 951
PciSI GCTCTTC 1 cut(s) 999
PctI GAATGC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 471
PfeI GAWTC 3 cut(s) 313, 752, 1025
PflFI GACNNNGTC 1 cut(s) 95
PscI ACATGT 1 cut(s) 951
Psp6I CCWGG 3 cut(s) 258, 663, 1099
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 3 cut(s) 258, 663, 1099
PspN4I GGNNCC 3 cut(s) 115, 657, 669
PspPI GGNCC 4 cut(s) 289, 524, 714, 895
PspXI VCTCGAGB 1 cut(s) 8
PstI CTGCAG 2 cut(s) 622, 891
PstNI CAGNNNCTG 3 cut(s) 611, 614, 1166
PsuI RGATCY 2 cut(s) 655, 667
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 546, 556
RsaNI GTAC 2 cut(s) 545, 555
RseI CAYNNNNRTG 2 cut(s) 275, 950
SapI GCTCTTC 1 cut(s) 999
SaqAI TTAA 2 cut(s) 354, 395
Sau3AI GATC 7 cut(s) 21, 202, 655, 667, 973, 1063, 1117
Sau96I GGNCC 4 cut(s) 289, 524, 714, 895
ScrFI CCNGG 3 cut(s) 260, 665, 1101
SduI GDGCHC 2 cut(s) 9, 535
SfaNI GCATC 2 cut(s) 36, 790
SfcI CTRYAG 2 cut(s) 618, 887
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 524
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 275, 950
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
SpeI ACTAGT 1 cut(s) 197
SphI GCATGC 1 cut(s) 229
Sse9I AATT 4 cut(s) 34, 284, 427, 1052
SsiI CCGC 1 cut(s) 111
SspMI CTAG 4 cut(s) 198, 464, 989, 1032
StyD4I CCNGG 3 cut(s) 258, 663, 1099
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 145, 233, 407
TaqI TCGA 2 cut(s) 9, 908
TasI AATT 4 cut(s) 34, 284, 427, 1052
TatI WGTACW 2 cut(s) 544, 554
TfiI GAWTC 3 cut(s) 313, 752, 1025
Tru1I TTAA 2 cut(s) 354, 395
Tru9I TTAA 2 cut(s) 354, 395
TscAI CASTG 4 cut(s) 150, 412, 829, 952
TspDTI ATGAA 7 cut(s) 269, 375, 459, 500, 853, 930, 1197
TspRI CASTG 4 cut(s) 150, 412, 829, 952
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 524
XceI RCATGY 2 cut(s) 229, 955
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 4 cut(s) 198, 464, 989, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.