Rh2BG130000

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001533
Physical Location & Seq
Reverse (-)
11134877 .. 11140020
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG130000.1

Sequence Viewer

Length: 1041 bp
ATGATCTGCATTGATAATTCGGAGTGGATGCGGAACGGCGACTACGCTCCTTCGAGATTTCAAGCTCAGGCCGACGCCGTCAATCTGATTTGTGGAGCTAAAACCCAGTCTAATCCGGAGAATACAGTAGGAGTTCTCACCATGGCAGGGAAACGTGTTCGCGTTTTGGTAACTCCTACTAGTGATCTTGGCAAGATTTTGGCGTGCATGCACGGTTTAGAAATAGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCCCTCAAACATCGCCAGAATAAGAAGCAGCAGCAAAGGATTATAGTCTTTGCTGGAAGTGTTGTTAAACATGAAAAGAAGACTTTGGAGATGATTGGAAGAAAGTTAAAGAAGAACAGTGTAGCCCTTGATATCATTAATTTTGGTGAAGAAGATGAGGAAAAGTCAGAGAAGCTAGAAGCACTTCTTGCTGCTGTGAACAATAATGACACCAGCCACATTGTTCATGTTCCGGCTGGTCCAAGTGCTCTTTCTGATGTACTCATAAGTACACCTATCTTCACCGGAGATGGAGAAGGTGGGAGTGGTTTTGCAGCAGCAGCAGCAGCTGCTGCAGCTGGTGGTGTATCTGGTTTTGATTTCGGTGTGGATCCAAACCTGGATCCTGAACTTGCCCTTGCTCTTAGAGTATCAATGGAGGAGGAGAGGGCCAGACAAGAAGCAGCTGCTAAAAAGGCTGCTGAGGATTCTTCCAAACAGGAGAAAGGAGGGGAAGAACAATCCAGCTCACAAGATGCAACTATGACAGAGCGTGCCAGTGCTGTAACTTCTGAAGCTGAGAATAAGAGTGTTGATTTCATGGATGATGACAACGCTCTGCTACAGCAGGCCCTTGCAATGTCGATGGATGAGCCTGCCTCCAGCCATGATTTGCGAGACACTGACATGTCTGAGGTAGTTGCAGATGATCCCGAGTTGGCTCTAGGTGAGTTCTCAACTTTGTACGAGCAATACTGCATTCGTTTACTGGCGCATTCTCACGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

346

Amino Acids

36.93

Weight (kDa)

4.71

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 1 - 109 6.6e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 1 cut(s) 31
AclWI GGATC 5 cut(s) 635, 647, 648, 660, 953
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 3 cut(s) 531, 541, 995
AfiI CCNNNNNNNGG 1 cut(s) 147
AflIII ACRYGT 2 cut(s) 154, 936
AgsI TTSAA 1 cut(s) 62
AhlI ACTAGT 1 cut(s) 179
AjnI CCWGG 2 cut(s) 240, 648
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 8 cut(s) 65, 98, 445, 599, 608, 716, 777, 827
AluI AGCT 8 cut(s) 65, 98, 445, 599, 608, 716, 777, 827
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 921
AlwI GGATC 5 cut(s) 635, 647, 648, 660, 953
AlwNI CAGNNNCTG 2 cut(s) 599, 602
Ama87I CYCGRG 1 cut(s) 962
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 4 cut(s) 69, 270, 699, 879
AseI ATTAAT 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 4 cut(s) 271, 509, 699, 880
AsuHPI GGTGA 5 cut(s) 130, 242, 428, 544, 989
AvaI CYCGRG 1 cut(s) 962
AvaII GGWCC 1 cut(s) 509
BamHI GGATCC 2 cut(s) 640, 652
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 520
BbvCI CCTCAGC 1 cut(s) 732
BccI CCATC 2 cut(s) 554, 889
BceAI ACGGC 2 cut(s) 52, 62
BciT130I CCWGG 2 cut(s) 242, 650
BcoDI GTCTC 1 cut(s) 921
BcuI ACTAGT 1 cut(s) 179
BfaI CTAG 3 cut(s) 180, 446, 974
BfmI CTRYAG 2 cut(s) 603, 872
Bme1390I CCNGG 2 cut(s) 242, 650
Bme18I GGWCC 1 cut(s) 509
BmeT110I CYCGRG 1 cut(s) 962
BmgT120I GGNCC 4 cut(s) 271, 509, 699, 880
BmiI GGNNCC 2 cut(s) 642, 654
BmrFI CCNGG 2 cut(s) 242, 650
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 2 cut(s) 18, 775
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 1 cut(s) 356
BplI GAGNNNNNCTC 2 cut(s) 893, 925
BpmI CTGGAG 1 cut(s) 895
Bpu10I CCTNAGC 2 cut(s) 66, 732
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 2 cut(s) 115, 554
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 3 cut(s) 106, 807, 1023
Bse3DI GCAATG 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 2 cut(s) 242, 650
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 33, 859, 904
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 4 cut(s) 80, 723, 819, 933
BseNI ACTGG 3 cut(s) 106, 807, 1023
BseRI GAGGAG 2 cut(s) 704, 707
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 4 cut(s) 71, 272, 701, 881
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 3 cut(s) 116, 503, 555
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 921
BsmI GAATGC 3 cut(s) 252, 1008, 1024
BsnI GGCC 4 cut(s) 71, 272, 701, 881
BsoBI CYCGRG 1 cut(s) 962
Bsp1286I GDGCHC 1 cut(s) 520
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 5 cut(s) 3, 184, 640, 652, 958
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 1 cut(s) 31
BspANI GGCC 4 cut(s) 71, 272, 701, 881
BspCNI CTCAG 4 cut(s) 79, 724, 820, 934
BspEI TCCGGA 1 cut(s) 115
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 2 cut(s) 642, 654
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 5 cut(s) 635, 647, 648, 660, 953
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 3 cut(s) 106, 807, 1023
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 5 cut(s) 3, 184, 640, 652, 958
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 242, 650
Bst4CI ACNGT 4 cut(s) 127, 215, 389, 1034
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 3 cut(s) 458, 599, 602
BstC8I GCNNGC 6 cut(s) 205, 209, 250, 804, 879, 906
BstDEI CTNAG 5 cut(s) 66, 674, 732, 828, 942
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 33, 859, 904
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 1024
BstKTI GATC 5 cut(s) 6, 187, 643, 655, 961
BstMAI GTCTC 1 cut(s) 921
BstMBI GATC 5 cut(s) 3, 184, 640, 652, 958
BstMWI GCNNNNNNNGC 9 cut(s) 269, 458, 590, 593, 596, 599, 602, 605, 725
BstNI CCWGG 2 cut(s) 242, 650
BstNSI RCATGY 2 cut(s) 211, 940
BstSCI CCNGG 2 cut(s) 240, 648
BstSFI CTRYAG 2 cut(s) 603, 872
BstUI CGCG 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 2 cut(s) 640, 652
BstYI RGATCY 2 cut(s) 640, 652
BsuRI GGCC 4 cut(s) 71, 272, 701, 881
BtgI CCRYGG 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 3 cut(s) 33, 859, 904
BtsIMutI CAGTG 3 cut(s) 394, 814, 930
Cac8I GCNNGC 6 cut(s) 205, 209, 250, 804, 879, 906
CaiI CAGNNNCTG 2 cut(s) 599, 602
CfoI GCGC 1 cut(s) 1024
Cfr13I GGNCC 4 cut(s) 271, 509, 699, 880
CseI GACGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 530, 540, 994
CviAII CATG 7 cut(s) 142, 208, 341, 497, 850, 917, 937
CviQI GTAC 3 cut(s) 530, 540, 994
DdeI CTNAG 5 cut(s) 66, 674, 732, 828, 942
DpnI GATC 5 cut(s) 5, 186, 642, 654, 960
DpnII GATC 5 cut(s) 3, 184, 640, 652, 958
Eco130I CCWWGG 1 cut(s) 141
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 962
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 240, 648
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 145, 211, 344, 500, 853, 920, 940
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FatI CATG 7 cut(s) 141, 207, 340, 496, 849, 916, 936
FokI GGATG 3 cut(s) 40, 866, 911
FspBI CTAG 3 cut(s) 180, 446, 974
GlaI GCGC 1 cut(s) 1023
GsuI CTGGAG 1 cut(s) 895
HaeIII GGCC 4 cut(s) 71, 272, 701, 881
HapII CCGG 3 cut(s) 116, 503, 555
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 1024
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 7 cut(s) 145, 211, 344, 500, 853, 920, 940
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HinfI GANTC 1 cut(s) 737
HpaII CCGG 3 cut(s) 116, 503, 555
HphI GGTGA 5 cut(s) 130, 242, 428, 544, 989
Hpy166II GTNNAC 3 cut(s) 469, 542, 1016
Hpy188I TCNGA 6 cut(s) 22, 87, 439, 526, 823, 943
Hpy188III TCNNGA 4 cut(s) 54, 116, 656, 962
Hpy8I GTNNAC 3 cut(s) 469, 542, 1016
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 60, 560
HpyCH4III ACNGT 4 cut(s) 127, 215, 389, 1034
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 9 cut(s) 9, 207, 211, 584, 605, 788, 887, 953, 1008
HpyF10VI GCNNNNNNNGC 9 cut(s) 269, 458, 590, 593, 596, 599, 602, 605, 725
HpyF3I CTNAG 5 cut(s) 66, 674, 732, 828, 942
HpySE526I ACGT 1 cut(s) 154
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 7 cut(s) 145, 211, 344, 500, 853, 920, 940
HspAI GCGC 1 cut(s) 1022
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 5 cut(s) 3, 184, 640, 652, 958
LmnI GCTCC 2 cut(s) 52, 95
LweI GCATC 2 cut(s) 18, 775
MaeI CTAG 3 cut(s) 180, 446, 974
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 169, 814
MalI GATC 5 cut(s) 5, 186, 642, 654, 960
MboI GATC 5 cut(s) 3, 184, 640, 652, 958
MboII GAAGA 8 cut(s) 361, 381, 394, 431, 434, 541, 732, 776
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 2 cut(s) 640, 652
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 3 cut(s) 16, 266, 409
MnlI CCTC 9 cut(s) 284, 421, 682, 685, 690, 727, 752, 919, 937
MroI TCCGGA 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 3 cut(s) 336, 377, 408
MslI CAYNNNNRTG 2 cut(s) 257, 935
MspA1I CMGCKG 3 cut(s) 599, 608, 716
MspI CCGG 3 cut(s) 116, 503, 555
MspR9I CCNGG 2 cut(s) 242, 650
MunI CAATTG 1 cut(s) 266
Mva1269I GAATGC 3 cut(s) 252, 1008, 1024
MvaI CCWGG 2 cut(s) 242, 650
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 9 cut(s) 269, 458, 590, 593, 596, 599, 602, 605, 725
NcoI CCATGG 1 cut(s) 141
NdeII GATC 5 cut(s) 3, 184, 640, 652, 958
NlaIII CATG 7 cut(s) 145, 211, 344, 500, 853, 920, 940
NlaIV GGNNCC 2 cut(s) 642, 654
NspI RCATGY 2 cut(s) 211, 940
PaeI GCATGC 1 cut(s) 211
PciI ACATGT 1 cut(s) 936
PctI GAATGC 3 cut(s) 252, 1008, 1024
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 737
PflFI GACNNNGTC 1 cut(s) 77
PscI ACATGT 1 cut(s) 936
PshBI ATTAAT 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 240, 648
PspGI CCWGG 2 cut(s) 240, 648
PspN4I GGNNCC 2 cut(s) 642, 654
PspPI GGNCC 4 cut(s) 271, 509, 699, 880
PsrI GAACNNNNNNTAC 2 cut(s) 26, 58
PstI CTGCAG 1 cut(s) 607
PstNI CAGNNNCTG 2 cut(s) 599, 602
PsuI RGATCY 2 cut(s) 640, 652
PsyI GACNNNGTC 1 cut(s) 77
PvuII CAGCTG 3 cut(s) 599, 608, 716
RsaI GTAC 3 cut(s) 531, 541, 995
RsaNI GTAC 3 cut(s) 530, 540, 994
RseI CAYNNNNRTG 2 cut(s) 257, 935
SaqAI TTAA 3 cut(s) 336, 377, 408
Sau3AI GATC 5 cut(s) 3, 184, 640, 652, 958
Sau96I GGNCC 4 cut(s) 271, 509, 699, 880
ScrFI CCNGG 2 cut(s) 242, 650
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 2 cut(s) 18, 775
SfcI CTRYAG 2 cut(s) 603, 872
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 2 cut(s) 257, 935
SpeI ACTAGT 1 cut(s) 179
SphI GCATGC 1 cut(s) 211
Sse9I AATT 3 cut(s) 16, 266, 409
SsiI CCGC 1 cut(s) 31
SspMI CTAG 3 cut(s) 180, 446, 974
StyD4I CCNGG 2 cut(s) 240, 648
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 127, 215, 389, 1034
TaiI ACGT 1 cut(s) 157
TaqI TCGA 2 cut(s) 53, 893
TasI AATT 3 cut(s) 16, 266, 409
TatI WGTACW 2 cut(s) 529, 539
TfiI GAWTC 1 cut(s) 737
Tru1I TTAA 3 cut(s) 336, 377, 408
Tru9I TTAA 3 cut(s) 336, 377, 408
TscAI CASTG 3 cut(s) 394, 814, 937
TspDTI ATGAA 4 cut(s) 251, 357, 485, 838
TspRI CASTG 3 cut(s) 394, 814, 937
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 509
VspI ATTAAT 1 cut(s) 408
XceI RCATGY 2 cut(s) 211, 940
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 180, 446, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.