RchiOBHm_Chr6g0252421

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001533
Physical Location & Seq
Reverse (-)
7553377 .. 7556463
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22633

Sequence Viewer

Length: 954 bp
ATGAACAAGCAGGCTACTATGATATGCGTCGACAATTCAGAATGGATGCGAAACGGTGATTACTCCCCTTCTCGATTCCAAGCTCAAGCCGATGCTATCAATCTCATCTGTGGTGCCAAAACCCAGGTTAAAGATTCAATCTTTTTCACTAAACCTTGCCAATCCGGAAAACACAGTTGGGGTGTTGACAATGGCGGCAAAGGGGTTCGTGTATTGGCTACACCCACTTCTGATCTTGGCAGAATTTTGGCTTGCATGCACGGTATTGGAAATGATAGGGAAGAAATTGAAGAAGAACAGTGTGCTCTTGATATTGTTGATTTTGGCGAAGAAGATGATGGAAAGCCAGAGAAGCTGGAGGCCCTTCTTTCTGCTGTTAACAATAACGATAGTAGTCATTTAGTACATGTCCCTCCTGGTCCAGATGCTCTCTCTGATGTTCTTATAAGTACACCTGTATTCACTGGTGATGGAGAAGGAGGAAGTGGCTTTGCAGTGGCAGCTGCAGCAGCTACTGCTGCTGCAATTGGTGGTTCTTCCGGCTATGATTTTGACGTAGACCCTAATATAGATCCTGAGCTTGCTCTTGCTCTTAGAGTTTCTATGGAAGAGGAAAGGGCAAGGCAAGAAGCTGCTGCCAAAAGATCGGCTGACGAAGCTAGTAGTAGACAAGGAGAGGAACCGCCATCCAAATCAGAGGATGTAACCATGACAGAAAGTGGCTCTGCAATGTCAATGAATATGTCTGGATCCGGTAATTCAGCAGGTGATGCTGAAATGTCAGAGGCAACTAGTGCAGATCAGGAGTTGGCTCTAGCTTCCAGGAGTGACCCCAATGATCCTTCAGTGAAAGAGCTTCTTGCATCTCTGAAGAATCAGTCCCAAGAAAGGATGAAGAGGGTCCATCAAAGGAGGACAAATGAAGATGTGACACCTTGTGTGCTTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

317

Amino Acids

33.62

Weight (kDa)

4.52

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 92 5.6e-13 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AarI CACCTGC 1 cut(s) 755
Acc36I ACCTGC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 3 cut(s) 30, 558, 667
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 2 cut(s) 195, 683
AclWI GGATC 4 cut(s) 566, 744, 757, 833
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 2 cut(s) 828, 890
AdeI CACNNNGTG 1 cut(s) 938
AfaI GTAC 2 cut(s) 405, 451
AfiI CCNNNNNNNGG 1 cut(s) 888
AflII CTTAAG 1 cut(s) 944
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 2 cut(s) 138, 290
AhlI ACTAGT 1 cut(s) 791
AjnI CCWGG 3 cut(s) 123, 415, 821
AjuI GAANNNNNNNTTGG 2 cut(s) 160, 192
AluBI AGCT 9 cut(s) 83, 355, 503, 512, 580, 632, 659, 818, 856
AluI AGCT 9 cut(s) 83, 355, 503, 512, 580, 632, 659, 818, 856
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 4 cut(s) 566, 744, 757, 833
AlwNI CAGNNNCTG 1 cut(s) 515
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 8 cut(s) 500, 503, 506, 509, 518, 521, 632, 635
ApoI RAATTY 1 cut(s) 243
AspS9I GGNCC 3 cut(s) 361, 419, 901
AsuHPI GGTGA 3 cut(s) 68, 479, 779
AvaII GGWCC 2 cut(s) 419, 901
BamHI GGATCC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 8 cut(s) 490, 505, 508, 512, 518, 521, 619, 622
BccI CCATC 4 cut(s) 332, 464, 694, 912
BciT130I CCWGG 3 cut(s) 125, 417, 823
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 3 cut(s) 660, 792, 815
BfmI CTRYAG 1 cut(s) 504
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 755
BisI GCNGC 9 cut(s) 196, 501, 504, 507, 510, 519, 522, 633, 636
BlsI GCNGC 9 cut(s) 197, 502, 505, 508, 511, 520, 523, 634, 637
Bme1390I CCNGG 3 cut(s) 125, 417, 823
Bme18I GGWCC 2 cut(s) 419, 901
BmgT120I GGNCC 3 cut(s) 361, 419, 901
BmiI GGNNCC 4 cut(s) 115, 681, 751, 902
BmrFI CCNGG 3 cut(s) 125, 417, 823
BmsI GCATC 5 cut(s) 36, 82, 415, 760, 872
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 164, 752
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse1I ACTGG 1 cut(s) 469
Bse3DI GCAATG 1 cut(s) 735
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 3 cut(s) 125, 417, 823
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 4 cut(s) 51, 686, 706, 897
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 1 cut(s) 469
BseXI GCAGC 8 cut(s) 490, 505, 508, 512, 518, 521, 619, 622
BsgI GTGCAG 1 cut(s) 816
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 3 cut(s) 165, 540, 753
BslFI GGGAC 2 cut(s) 395, 865
BslI CCNNNNNNNGG 1 cut(s) 888
BsmFI GGGAC 2 cut(s) 395, 865
BsnI GGCC 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 307
Bsp13I TCCGGA 1 cut(s) 164
Bsp143I GATC 6 cut(s) 232, 571, 644, 749, 799, 838
BspACI CCGC 2 cut(s) 195, 683
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 1 cut(s) 568
BspEI TCCGGA 1 cut(s) 164
BspLI GGNNCC 4 cut(s) 115, 681, 751, 902
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 755
BspPI GGATC 4 cut(s) 566, 744, 757, 833
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 6 cut(s) 232, 571, 644, 749, 799, 838
Bst2UI CCWGG 3 cut(s) 125, 417, 823
Bst4CI ACNGT 4 cut(s) 56, 176, 263, 300
Bst6I CTCTTC 2 cut(s) 603, 890
BstAFI CTTAAG 1 cut(s) 944
BstAPI GCANNNNNTGC 3 cut(s) 515, 770, 794
BstC8I GCNNGC 4 cut(s) 12, 253, 257, 582
BstDEI CTNAG 2 cut(s) 576, 593
BstF5I GGATG 4 cut(s) 51, 686, 706, 897
BstKTI GATC 6 cut(s) 235, 574, 647, 752, 802, 841
BstMBI GATC 6 cut(s) 232, 571, 644, 749, 799, 838
BstMWI GCNNNNNNNGC 9 cut(s) 352, 500, 506, 509, 515, 518, 656, 770, 794
BstNI CCWGG 3 cut(s) 125, 417, 823
BstNSI RCATGY 2 cut(s) 259, 410
BstSCI CCNGG 3 cut(s) 123, 415, 821
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 8 cut(s) 490, 505, 508, 512, 518, 521, 619, 622
BstX2I RGATCY 2 cut(s) 571, 749
BstYI RGATCY 2 cut(s) 571, 749
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 4 cut(s) 51, 686, 706, 897
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 4 cut(s) 305, 462, 501, 852
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 4 cut(s) 12, 253, 257, 582
CaiI CAGNNNCTG 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 361, 419, 901
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 404, 450
CviAII CATG 3 cut(s) 256, 407, 709
CviQI GTAC 2 cut(s) 404, 450
DdeI CTNAG 2 cut(s) 576, 593
DpnI GATC 6 cut(s) 234, 573, 646, 751, 801, 840
DpnII GATC 6 cut(s) 232, 571, 644, 749, 799, 838
DraIII CACNNNGTG 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 603, 890
EarI CTCTTC 2 cut(s) 603, 890
Eco47I GGWCC 2 cut(s) 419, 901
Eco57I CTGAAG 2 cut(s) 828, 890
EcoO109I RGGNCCY 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 123, 415, 821
FaeI CATG 3 cut(s) 259, 410, 712
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FaqI GGGAC 2 cut(s) 395, 865
FatI CATG 3 cut(s) 255, 406, 708
FblI GTMKAC 3 cut(s) 30, 558, 667
Fnu4HI GCNGC 9 cut(s) 196, 501, 504, 507, 510, 519, 522, 633, 636
FokI GGATG 4 cut(s) 58, 673, 713, 904
Fsp4HI GCNGC 9 cut(s) 196, 501, 504, 507, 510, 519, 522, 633, 636
FspBI CTAG 3 cut(s) 660, 792, 815
GluI GCNGC 9 cut(s) 196, 501, 504, 507, 510, 519, 522, 633, 636
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 1 cut(s) 362
HapII CCGG 3 cut(s) 165, 540, 753
HgaI GACGC 1 cut(s) 16
Hin1II CATG 3 cut(s) 259, 410, 712
HincII GTYRAC 3 cut(s) 31, 187, 379
HindII GTYRAC 3 cut(s) 31, 187, 379
HinfI GANTC 3 cut(s) 75, 134, 874
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 3 cut(s) 165, 540, 753
HphI GGTGA 3 cut(s) 68, 479, 779
Hpy166II GTNNAC 6 cut(s) 31, 187, 379, 452, 559, 668
Hpy188I TCNGA 6 cut(s) 40, 232, 436, 697, 784, 870
Hpy188III TCNNGA 7 cut(s) 72, 165, 308, 422, 575, 747, 803
Hpy8I GTNNAC 6 cut(s) 31, 187, 379, 452, 559, 668
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 4 cut(s) 78, 374, 470, 852
HpyCH4III ACNGT 4 cut(s) 56, 176, 263, 300
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 8 cut(s) 255, 259, 494, 506, 524, 728, 797, 863
HpyF10VI GCNNNNNNNGC 9 cut(s) 352, 500, 506, 509, 515, 518, 656, 770, 794
HpyF3I CTNAG 2 cut(s) 576, 593
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 3 cut(s) 259, 410, 712
Kpn2I TCCGGA 1 cut(s) 164
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 6 cut(s) 232, 571, 644, 749, 799, 838
Lsp1109I GCAGC 8 cut(s) 490, 505, 508, 512, 518, 521, 619, 622
LweI GCATC 5 cut(s) 36, 82, 415, 760, 872
MaeI CTAG 3 cut(s) 660, 792, 815
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 3 cut(s) 703, 827, 928
MalI GATC 6 cut(s) 234, 573, 646, 751, 801, 840
MboI GATC 6 cut(s) 232, 571, 644, 749, 799, 838
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 2 cut(s) 571, 749
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 5 cut(s) 34, 243, 285, 525, 757
MnlI CCTC 9 cut(s) 352, 423, 473, 604, 670, 691, 778, 891, 906
MroI TCCGGA 1 cut(s) 164
MseI TTAA 3 cut(s) 129, 378, 945
MspA1I CMGCKG 1 cut(s) 503
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 3 cut(s) 165, 540, 753
MspR9I CCNGG 3 cut(s) 125, 417, 823
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 3 cut(s) 125, 417, 823
MwoI GCNNNNNNNGC 9 cut(s) 352, 500, 506, 509, 515, 518, 656, 770, 794
NdeII GATC 6 cut(s) 232, 571, 644, 749, 799, 838
NlaIII CATG 3 cut(s) 259, 410, 712
NlaIV GGNNCC 4 cut(s) 115, 681, 751, 902
NmuCI GTSAC 2 cut(s) 827, 928
NspI RCATGY 2 cut(s) 259, 410
PaeI GCATGC 1 cut(s) 259
PaqCI CACCTGC 1 cut(s) 755
PciI ACATGT 1 cut(s) 406
PfeI GAWTC 3 cut(s) 75, 134, 874
PfoI TCCNGGA 1 cut(s) 821
PkrI GCNGC 9 cut(s) 197, 502, 505, 508, 511, 520, 523, 634, 637
PscI ACATGT 1 cut(s) 406
PsiI TTATAA 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 123, 415, 821
PspGI CCWGG 3 cut(s) 123, 415, 821
PspN4I GGNNCC 4 cut(s) 115, 681, 751, 902
PspPI GGNCC 3 cut(s) 361, 419, 901
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 2 cut(s) 571, 749
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 2 cut(s) 405, 451
RsaNI GTAC 2 cut(s) 404, 450
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 3 cut(s) 129, 378, 945
SatI GCNGC 9 cut(s) 196, 501, 504, 507, 510, 519, 522, 633, 636
Sau3AI GATC 6 cut(s) 232, 571, 644, 749, 799, 838
Sau96I GGNCC 3 cut(s) 361, 419, 901
ScrFI CCNGG 3 cut(s) 125, 417, 823
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 5 cut(s) 36, 82, 415, 760, 872
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 2 cut(s) 419, 901
SmlI CTYRAG 2 cut(s) 84, 944
SmoI CTYRAG 2 cut(s) 84, 944
SpeI ACTAGT 1 cut(s) 791
SphI GCATGC 1 cut(s) 259
Sse9I AATT 5 cut(s) 34, 243, 285, 525, 757
SsiI CCGC 2 cut(s) 195, 683
SspMI CTAG 3 cut(s) 660, 792, 815
StyD4I CCNGG 3 cut(s) 123, 415, 821
TaaI ACNGT 4 cut(s) 56, 176, 263, 300
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 30, 73
TasI AATT 5 cut(s) 34, 243, 285, 525, 757
TatI WGTACW 2 cut(s) 403, 449
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 3 cut(s) 75, 134, 874
Tru1I TTAA 3 cut(s) 129, 378, 945
Tru9I TTAA 3 cut(s) 129, 378, 945
TscAI CASTG 4 cut(s) 305, 469, 501, 852
TseFI GTSAC 2 cut(s) 827, 928
TseI GCWGC 8 cut(s) 500, 503, 506, 509, 518, 521, 632, 635
Tsp45I GTSAC 2 cut(s) 827, 928
TspDTI ATGAA 4 cut(s) 17, 752, 908, 936
TspRI CASTG 4 cut(s) 305, 469, 501, 852
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 2 cut(s) 419, 901
XapI RAATTY 1 cut(s) 243
XceI RCATGY 2 cut(s) 259, 410
XmiI GTMKAC 3 cut(s) 30, 558, 667
XspI CTAG 3 cut(s) 660, 792, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.