Rw6G031120

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001533
Physical Location & Seq
Reverse (-)
55158753 .. 55160130
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031120.1

Sequence Viewer

Length: 1188 bp
ATGGTTCTCGAGGCGACTATGATATGCATCGACAATTCAGAATGGATGCGAAACAGTGATTACTCTCCCTCCCGATTCCAAGCTCAAGCCGATGCTGTGAATCTCATCTGTGGTATCAAAACCCAGGCCAACCCGGAAAACACAGTTGGGGTGTTGACAATGGCGGGCAAAGGGGTTCGCGTGCTGACCACAACCACCTCTGACCTTGGCAAGATTTTGACTTGCACGCATGGCCTCGAGATAGGGGGTGAGATGAACATTGCAGCTGCAATCCAGGTAGCTCAGTTGGCTCTTAAGCATCGCCAAAATAAAAACCAACAACAAAGGATTGTAGTATTTGCTGGAAGTCCTGTCAAGAATGACAAGAAGGCATTGGAAATAATTGGGAAGAAACTGAAGAAGAACAGTGTTGCTCTTGACATTGTCGATTTTGGAGGAGAAGATGATGGAAAAGCAGAGAAGCTGGAGGCCCTTGTTTCTGCTGTTAATAATAATGGCACTAGTCACATAGTCCATGTACCTCCTGGTCTCCATGCTCTCTCTGATGTTCTTTTAAGTACACCTATATTAACTGGTGATGAAGAAGGAGGAGCTGGCTTTGCAGCCACAGCCGCAGCAGCTGGTATTGGTGGTTCTTCTGGCCATGACTTTGGAATAGATCCGAATATAGATCCCGAGCTTGCTCTCGCCCTTAGAATGTCAATGGAAGAGGAAAGGGCAAGACAAGAAGCTGCTGCTGCCAAAAGAGCTGAGGAAGAAGCTGCTAGCAGACGAGGAAAAGGAGAGGAACGGCCATCCAAATCGGAGGATGTGACCATAACAGAAAGCGATGATGTTGAAATGGTTGATGAGAATTCCTTGCTAATGCAGGCTCTTGCGATGACAATGAATAACTATGGCTCTAGTAGTTCTGCAGTTGGTGATGCTGAGATGTCAGAGGCAACTGGTGTGGATCAGGACTTGGCTCTAGCTCTTCAAATGTCCATGCAGGAAAGCGCAGGAGGGGCATCATCATCCGAGACGGTTGATGTGAGCAAGGTGTTGGAGGATCAGTCTTTTCTTTCGTCTATCCTGGAATCTCTTCCGGGAGTTGACCCCAATGATCCTTCTGTGGAAGAGTTTCTTGCATCTGTGATTAATCAGTTTAAAAAGAATGAAGAAGATCCATCTAATGACGAGGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

395

Amino Acids

41.76

Weight (kDa)

4.48

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 1.2e-31 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 180
AciI CCGC 2 cut(s) 164, 612
AclWI GGATC 6 cut(s) 653, 665, 960, 1056, 1097, 1157
AcoI YGGCCR 2 cut(s) 640, 791
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 2 cut(s) 519, 559
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 839, 977
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 4 cut(s) 123, 273, 523, 1071
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
Alw26I GTCTC 2 cut(s) 533, 1013
AlwI GGATC 6 cut(s) 653, 665, 960, 1056, 1097, 1157
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 3 cut(s) 8, 236, 674
AoxI GGCC 5 cut(s) 126, 232, 468, 640, 791
ApeKI GCWGC 9 cut(s) 263, 266, 602, 614, 617, 731, 734, 737, 761
ApoI RAATTY 1 cut(s) 853
AseI ATTAAT 1 cut(s) 1137
Asp700I GAANNNNTTC 2 cut(s) 1080, 1119
AspLEI GCGC 1 cut(s) 998
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 2 cut(s) 134, 1086
AsuHPI GGTGA 3 cut(s) 260, 587, 932
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 3 cut(s) 8, 236, 674
BalI TGGCCA 1 cut(s) 642
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
BccI CCATC 3 cut(s) 440, 802, 1174
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 4 cut(s) 125, 275, 525, 1073
BcnI CCSGG 2 cut(s) 134, 1086
BcoDI GTCTC 2 cut(s) 533, 1013
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 4 cut(s) 501, 765, 903, 968
BfmI CTRYAG 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 293
Bme1390I CCNGG 6 cut(s) 125, 134, 275, 525, 1073, 1086
BmeT110I CYCGRG 3 cut(s) 8, 236, 674
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 6 cut(s) 125, 134, 275, 525, 1073, 1086
BmsI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1016, 1136
BmtI GCTAGC 1 cut(s) 768
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 134, 1086
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 2 cut(s) 123, 205
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 1037, 1067
Bse1I ACTGG 2 cut(s) 577, 949
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 4 cut(s) 125, 275, 525, 1073
BseDI CCNNGG 2 cut(s) 123, 205
BseGI GGATG 4 cut(s) 51, 794, 814, 1013
BseJI GATNNNNATC 1 cut(s) 26
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 3 cut(s) 296, 741, 918
BseNI ACTGG 2 cut(s) 577, 949
BseRI GAGGAG 2 cut(s) 450, 603
BseXI GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 128, 234, 470, 642, 793
BsiHKCI CYCGRG 3 cut(s) 8, 236, 674
BsiSI CCGG 2 cut(s) 134, 1085
BsmAI GTCTC 2 cut(s) 533, 1013
BsmBI CGTCTC 1 cut(s) 1013
BsnI GGCC 5 cut(s) 128, 234, 470, 642, 793
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 3 cut(s) 8, 236, 674
Bsp143I GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
BspACI CCGC 2 cut(s) 164, 612
BspANI GGCC 5 cut(s) 128, 234, 470, 642, 793
BspCNI CTCAG 3 cut(s) 295, 742, 919
BspFNI CGCG 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 916
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 6 cut(s) 653, 665, 960, 1056, 1097, 1157
BspQI GCTCTTC 1 cut(s) 978
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 2 cut(s) 577, 949
BssECI CCNNGG 2 cut(s) 123, 205
BssMI GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 4 cut(s) 125, 275, 525, 1073
Bst4CI ACNGT 4 cut(s) 56, 145, 407, 1024
Bst6I CTCTTC 4 cut(s) 702, 978, 1086, 1110
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 7 cut(s) 166, 182, 227, 595, 681, 766, 870
BstDEI CTNAG 4 cut(s) 282, 692, 750, 927
BstF5I GGATG 4 cut(s) 51, 794, 814, 1013
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 998
BstKTI GATC 6 cut(s) 661, 673, 955, 1051, 1105, 1165
BstMAI GTCTC 2 cut(s) 533, 1013
BstMBI GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
BstMWI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1004
BstNI CCWGG 4 cut(s) 125, 275, 525, 1073
BstSCI CCNGG 6 cut(s) 123, 132, 273, 523, 1071, 1084
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
BstX2I RGATCY 3 cut(s) 658, 670, 1162
BstXI CCANNNNNNTGG 1 cut(s) 650
BstYI RGATCY 3 cut(s) 658, 670, 1162
BsuRI GGCC 5 cut(s) 128, 234, 470, 642, 793
BtgZI GCGATG 3 cut(s) 284, 843, 893
BtsCI GGATG 4 cut(s) 51, 794, 814, 1013
BtsIMutI CAGTG 2 cut(s) 61, 412
Cac8I GCNNGC 7 cut(s) 166, 182, 227, 595, 681, 766, 870
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 998
Cfr13I GGNCC 1 cut(s) 469
Csp6I GTAC 2 cut(s) 518, 558
CspCI CAANNNNNGTGG 2 cut(s) 930, 965
CviAII CATG 5 cut(s) 230, 515, 533, 644, 985
CviQI GTAC 2 cut(s) 518, 558
DdeI CTNAG 4 cut(s) 282, 692, 750, 927
DpnI GATC 6 cut(s) 660, 672, 954, 1050, 1104, 1164
DpnII GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
DraI TTTAAA 1 cut(s) 1147
EaeI YGGCCR 2 cut(s) 640, 791
Eam1104I CTCTTC 4 cut(s) 702, 978, 1086, 1110
EarI CTCTTC 4 cut(s) 702, 978, 1086, 1110
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 3 cut(s) 8, 236, 674
EcoO109I RGGNCCY 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 4 cut(s) 123, 273, 523, 1071
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 1013
FaeI CATG 5 cut(s) 233, 518, 536, 647, 988
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FatI CATG 5 cut(s) 229, 514, 532, 643, 984
FauI CCCGC 1 cut(s) 157
FokI GGATG 4 cut(s) 58, 781, 821, 1000
FspBI CTAG 4 cut(s) 501, 765, 903, 968
GlaI GCGC 1 cut(s) 997
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 5 cut(s) 128, 234, 470, 642, 793
HapII CCGG 2 cut(s) 134, 1085
HhaI GCGC 1 cut(s) 998
Hin1II CATG 5 cut(s) 233, 518, 536, 647, 988
Hin6I GCGC 1 cut(s) 996
HinP1I GCGC 1 cut(s) 996
HincII GTYRAC 2 cut(s) 156, 1093
HindII GTYRAC 2 cut(s) 156, 1093
HinfI GANTC 3 cut(s) 75, 100, 1076
HpaII CCGG 2 cut(s) 134, 1085
HphI GGTGA 3 cut(s) 260, 587, 932
Hpy166II GTNNAC 3 cut(s) 156, 560, 1093
Hpy188I TCNGA 7 cut(s) 40, 202, 544, 663, 805, 937, 1018
Hpy188III TCNNGA 7 cut(s) 8, 72, 238, 355, 416, 674, 956
Hpy8I GTNNAC 3 cut(s) 156, 560, 1093
HpyAV CCTTC 3 cut(s) 361, 578, 1116
HpyCH4III ACNGT 4 cut(s) 56, 145, 407, 1024
HpyCH4V TGCA 9 cut(s) 27, 225, 263, 269, 602, 868, 914, 988, 1127
HpyF10VI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1004
HpyF3I CTNAG 4 cut(s) 282, 692, 750, 927
Hsp92II CATG 5 cut(s) 233, 518, 536, 647, 988
HspAI GCGC 1 cut(s) 996
Kzo9I GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
LweI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1016, 1136
MaeI CTAG 4 cut(s) 501, 765, 903, 968
MaeIII GTNAC 2 cut(s) 503, 811
MalI GATC 6 cut(s) 660, 672, 954, 1050, 1104, 1164
MboI GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
MflI RGATCY 3 cut(s) 658, 670, 1162
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 3 cut(s) 34, 381, 853
MluNI TGGCCA 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 1023
Mox20I TGGCCA 1 cut(s) 642
Mph1103I ATGCAT 1 cut(s) 29
MroXI GAANNNNTTC 2 cut(s) 1080, 1119
MscI TGGCCA 1 cut(s) 642
MseI TTAA 6 cut(s) 294, 486, 554, 569, 1137, 1146
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 2 cut(s) 266, 620
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 2 cut(s) 134, 1085
MspR9I CCNGG 6 cut(s) 125, 134, 275, 525, 1073, 1086
MvaI CCWGG 4 cut(s) 125, 275, 525, 1073
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1004
NciI CCSGG 2 cut(s) 134, 1086
NdeII GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 5 cut(s) 233, 518, 536, 647, 988
NmuCI GTSAC 2 cut(s) 503, 811
NsiI ATGCAT 1 cut(s) 29
PaeR7I CTCGAG 2 cut(s) 8, 236
PciSI GCTCTTC 1 cut(s) 978
PdmI GAANNNNTTC 2 cut(s) 1080, 1119
PfeI GAWTC 3 cut(s) 75, 100, 1076
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 1071, 1084
PshBI ATTAAT 1 cut(s) 1137
Psp6I CCWGG 4 cut(s) 123, 273, 523, 1071
PspGI CCWGG 4 cut(s) 123, 273, 523, 1071
PspPI GGNCC 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 658, 670, 1162
PsyI GACNNNGTC 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 266, 620
RsaI GTAC 2 cut(s) 519, 559
RsaNI GTAC 2 cut(s) 518, 558
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 6 cut(s) 294, 486, 554, 569, 1137, 1146
Sau3AI GATC 6 cut(s) 658, 670, 952, 1048, 1102, 1162
Sau96I GGNCC 1 cut(s) 469
ScrFI CCNGG 6 cut(s) 125, 134, 275, 525, 1073, 1086
SfaNI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1016, 1136
SfcI CTRYAG 1 cut(s) 912
Sfr274I CTCGAG 2 cut(s) 8, 236
SlaI CTCGAG 2 cut(s) 8, 236
SmlI CTYRAG 4 cut(s) 8, 84, 236, 293
SmoI CTYRAG 4 cut(s) 8, 84, 236, 293
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 3 cut(s) 34, 381, 853
SsiI CCGC 2 cut(s) 164, 612
SspMI CTAG 4 cut(s) 501, 765, 903, 968
StyD4I CCNGG 6 cut(s) 123, 132, 273, 523, 1071, 1084
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 4 cut(s) 56, 145, 407, 1024
TaqI TCGA 4 cut(s) 9, 30, 237, 426
TasI AATT 3 cut(s) 34, 381, 853
TatI WGTACW 1 cut(s) 557
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 3 cut(s) 75, 100, 1076
Tru1I TTAA 6 cut(s) 294, 486, 554, 569, 1137, 1146
Tru9I TTAA 6 cut(s) 294, 486, 554, 569, 1137, 1146
TscAI CASTG 2 cut(s) 61, 412
TseFI GTSAC 2 cut(s) 503, 811
TseI GCWGC 9 cut(s) 263, 266, 602, 614, 617, 731, 734, 737, 761
Tsp45I GTSAC 2 cut(s) 503, 811
TspDTI ATGAA 4 cut(s) 269, 594, 902, 1170
TspRI CASTG 2 cut(s) 61, 412
Tth111I GACNNNGTC 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 1137
XapI RAATTY 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 2 cut(s) 8, 236
XmnI GAANNNNTTC 2 cut(s) 1080, 1119
XspI CTAG 4 cut(s) 501, 765, 903, 968
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.