MD15G1243700.v1.1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001533
Physical Location & Seq
Reverse (-)
20014312 .. 20018691
4380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1243700.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGTGCTCGAGGCGACAATGATCTGCATTGATAATTCGGAATGGATGCGGAACGGTGATTACGCTCCGTCCAGGTTTCTAGCTCAGGCCGATGCTGTTAATCTGATTTGCGGAGCTAAAACCCAGTCTAATCCGGAGAATACAGTAGGAATTCTCACCATGGCAGGAAAAGGTGTTCGTGTTTTGGTCACTCCAACCAGTGATCTTGGCAAGATTCTGGCATGCATGCATGGTTTAGAAATAGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCTCTTAAGCATCGCCAAAATAAGAAACAGCAACAGAGGATTATTGTGTTTGCTGGAAGTACTGTCAAACATGACAAAAAGCTTTTAGAGATGATTGGAAGGAAGTTAAAGAAGAACAGTGTGGCCCTTGATATTGTCAATTTTGGTGAAGAAGATGAAGCTAAGACAGAGAAGCTAGAAGCACTACTTGCTGCTGTGAACAATAATGACACCAGCCACATTGTTCATGTACCAGCAGGTCCAAGTGCTCTTTCTGATGTACTTATAAGTACACCTATCTTCACTGGAGATGGGGAAGGCGGAAGTGGCTTTGCAGCAGCAGCAGCTGCTGCTGCCGCAGGTGGTGTCTCTGGTTTTGATTTCGGTGTGGATCCAAACCTGGATCCTGAGCTTGCCCTTGCTCTTAGAGTTTCAATGGAAGAAGAGAGGGCCAGACAAGAAGCAGCTGCTAAAAAGGCATCCGAGGATGTTGCTAAACAGGAAAAAGGAGGGGAGGGGCAATCCAGCTCACAAGATGCAACGATGACTGATCGTGCCAGTGTTGGAACTTCTGAAGCTGAGAATAAGAGTAGTGATTTCATGGATGATGAGAATGCCCTGCTACAACAGGCCCTTGCAATGTCGATGGATGAACCTGTCTCCAGTGATGATATTCGAGACACAGATATGTCTGAGGCAACTGATCCAGAGTTGGCTCTAGCTCTTCAAATGTCTGTTCAGGAAGGGGAAAAGGATTCTGGTAGCGAGAAAGATATGAGTAAATTACTGGCCGACCAGTCTTTTGTGTCTTCCATCCTTGCCTCGCTTCCAGGGGTTGACCCAAATGATCCTTCAGTTAAAGATTTGCTTGCTTCAATGCAAAACCAGTCAGAGCCTGATCAGAAGAAGAACGAAGACAAACCACCAAAAGAGGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

404

Amino Acids

42.76

Weight (kDa)

4.55

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 2.8e-31 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 2 cut(s) 515, 617
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 4 cut(s) 49, 111, 588, 624
AclWI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 2 cut(s) 861, 1104
AfaI GTAC 4 cut(s) 349, 519, 549, 559
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 3 cut(s) 702, 995, 1143
AjnI CCWGG 4 cut(s) 71, 258, 666, 1096
AjuI GAANNNNNNNTTGG 4 cut(s) 523, 555, 1102, 1134
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw21I GWGCWC 2 cut(s) 9, 538
Alw26I GTCTC 3 cut(s) 640, 931, 939
AlwI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
AlwNI CAGNNNCTG 3 cut(s) 614, 617, 1163
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 5 cut(s) 87, 411, 717, 897, 1056
ApoI RAATTY 1 cut(s) 150
ArsI GACNNNNNNTTYG 2 cut(s) 1052, 1084
AspS9I GGNCC 4 cut(s) 412, 527, 717, 898
AsuHPI GGTGA 4 cut(s) 68, 148, 260, 446
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BamHI GGATCC 2 cut(s) 658, 670
BbsI GAAGAC 2 cut(s) 1068, 1188
Bbv12I GWGCWC 2 cut(s) 9, 538
BccI CCATC 3 cut(s) 572, 907, 1088
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 4 cut(s) 73, 260, 668, 1098
BclI TGATCA 1 cut(s) 1165
BcoDI GTCTC 3 cut(s) 640, 931, 939
BfaI CTAG 3 cut(s) 80, 464, 986
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 2 cut(s) 515, 617
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 4 cut(s) 73, 260, 668, 1098
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 4 cut(s) 412, 527, 717, 898
BmiI GGNNCC 2 cut(s) 660, 672
BmrFI CCNGG 4 cut(s) 73, 260, 668, 1098
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 5 cut(s) 36, 82, 307, 755, 793
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 2 cut(s) 1068, 1188
BpmI CTGGAG 2 cut(s) 594, 913
Bpu10I CCTNAGC 2 cut(s) 84, 675
BsaJI CCNNGG 3 cut(s) 159, 750, 1097
BsaWI WCCGGW 1 cut(s) 133
Bse1I ACTGG 8 cut(s) 124, 198, 577, 825, 930, 1059, 1063, 1153
Bse3DI GCAATG 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 4 cut(s) 73, 260, 668, 1098
BseDI CCNNGG 3 cut(s) 159, 750, 1097
BseGI GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 4 cut(s) 98, 666, 837, 951
BseNI ACTGG 8 cut(s) 124, 198, 577, 825, 930, 1059, 1063, 1153
BshFI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BsiHKAI GWGCWC 2 cut(s) 9, 538
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 1 cut(s) 134
BsmAI GTCTC 3 cut(s) 640, 931, 939
BsmI GAATGC 2 cut(s) 270, 886
BsnI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 9, 538
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 4 cut(s) 49, 111, 588, 624
BspANI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BspCNI CTCAG 4 cut(s) 97, 667, 838, 952
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 660, 672
BspMI ACCTGC 2 cut(s) 515, 617
BspPI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
BspQI GCTCTTC 1 cut(s) 996
BspTI CTTAAG 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 8 cut(s) 124, 198, 577, 825, 930, 1059, 1063, 1153
BssECI CCNNGG 3 cut(s) 159, 750, 1097
BssMI GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 4 cut(s) 73, 260, 668, 1098
Bst4CI ACNGT 4 cut(s) 56, 145, 352, 407
Bst6I CTCTTC 2 cut(s) 705, 996
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 3 cut(s) 476, 614, 617
BstC8I GCNNGC 5 cut(s) 223, 227, 268, 681, 1137
BstDEI CTNAG 6 cut(s) 84, 450, 675, 692, 846, 960
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BstKTI GATC 8 cut(s) 24, 205, 661, 673, 820, 973, 1117, 1168
BstMAI GTCTC 3 cut(s) 640, 931, 939
BstMBI GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
BstNI CCWGG 4 cut(s) 73, 260, 668, 1098
BstNSI RCATGY 2 cut(s) 225, 229
BstSCI CCNGG 4 cut(s) 71, 258, 666, 1096
BstV2I GAAGAC 2 cut(s) 1068, 1188
BstX2I RGATCY 2 cut(s) 658, 670
BstYI RGATCY 2 cut(s) 658, 670
BsuRI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BtsIMutI CAGTG 5 cut(s) 205, 412, 570, 832, 937
BveI ACCTGC 2 cut(s) 515, 617
Cac8I GCNNGC 5 cut(s) 223, 227, 268, 681, 1137
CaiI CAGNNNCTG 3 cut(s) 614, 617, 1163
Cfr13I GGNCC 4 cut(s) 412, 527, 717, 898
Csp6I GTAC 4 cut(s) 348, 518, 548, 558
CviAII CATG 7 cut(s) 160, 222, 226, 230, 359, 515, 868
CviQI GTAC 4 cut(s) 348, 518, 548, 558
DdeI CTNAG 6 cut(s) 84, 450, 675, 692, 846, 960
DpnI GATC 8 cut(s) 23, 204, 660, 672, 819, 972, 1116, 1167
DpnII GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 705, 996
EarI CTCTTC 2 cut(s) 705, 996
EciI GGCGGA 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 2 cut(s) 861, 1104
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 150
EcoRII CCWGG 4 cut(s) 71, 258, 666, 1096
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 2 cut(s) 227, 231
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 1119, 1151
FatI CATG 7 cut(s) 159, 221, 225, 229, 358, 514, 867
FbaI TGATCA 1 cut(s) 1165
FokI GGATG 6 cut(s) 58, 733, 767, 884, 929, 1067
FspBI CTAG 3 cut(s) 80, 464, 986
GsuI CTGGAG 2 cut(s) 594, 913
HaeIII GGCC 5 cut(s) 89, 413, 719, 899, 1058
HapII CCGG 1 cut(s) 134
Hin1II CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 214, 1022
HpaII CCGG 1 cut(s) 134
HphI GGTGA 4 cut(s) 68, 148, 260, 446
Hpy166II GTNNAC 3 cut(s) 487, 560, 1105
Hpy188I TCNGA 8 cut(s) 40, 105, 544, 751, 841, 961, 1159, 1170
Hpy188III TCNNGA 5 cut(s) 134, 674, 944, 974, 1007
Hpy8I GTNNAC 3 cut(s) 487, 560, 1105
HpyAV CCTTC 4 cut(s) 381, 578, 1004, 1128
HpyCH4III ACNGT 4 cut(s) 56, 145, 352, 407
HpyCH4V TGCA 7 cut(s) 27, 225, 229, 602, 806, 905, 1147
HpyF3I CTNAG 6 cut(s) 84, 450, 675, 692, 846, 960
Hsp92II CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 1165
Kzo9I GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 2 cut(s) 70, 113
LweI GCATC 5 cut(s) 36, 82, 307, 755, 793
MaeI CTAG 3 cut(s) 80, 464, 986
MaeIII GTNAC 1 cut(s) 187
MalI GATC 8 cut(s) 23, 204, 660, 672, 819, 972, 1116, 1167
MboI GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 2 cut(s) 658, 670
MhlI GDGCHC 2 cut(s) 9, 538
MluCI AATT 5 cut(s) 34, 150, 284, 427, 1049
MmeI TCCRAC 2 cut(s) 218, 811
Mph1103I ATGCAT 2 cut(s) 227, 231
MroI TCCGGA 1 cut(s) 133
MseI TTAA 4 cut(s) 99, 294, 395, 1125
MslI CAYNNNNRTG 2 cut(s) 275, 953
MspA1I CMGCKG 2 cut(s) 614, 734
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 4 cut(s) 73, 260, 668, 1098
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 2 cut(s) 270, 886
MvaI CCWGG 4 cut(s) 73, 260, 668, 1098
NcoI CCATGG 1 cut(s) 159
NdeII GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
NlaIII CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
NlaIV GGNNCC 2 cut(s) 660, 672
NmuCI GTSAC 1 cut(s) 187
NsiI ATGCAT 2 cut(s) 227, 231
NspI RCATGY 2 cut(s) 225, 229
PaeI GCATGC 2 cut(s) 225, 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 2 cut(s) 270, 886
PfeI GAWTC 2 cut(s) 214, 1022
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 4 cut(s) 71, 258, 666, 1096
PspGI CCWGG 4 cut(s) 71, 258, 666, 1096
PspN4I GGNNCC 2 cut(s) 660, 672
PspPI GGNCC 4 cut(s) 412, 527, 717, 898
PspXI VCTCGAGB 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 44, 76
PstNI CAGNNNCTG 3 cut(s) 614, 617, 1163
PsuI RGATCY 2 cut(s) 658, 670
PvuII CAGCTG 2 cut(s) 614, 734
RsaI GTAC 4 cut(s) 349, 519, 549, 559
RsaNI GTAC 4 cut(s) 348, 518, 548, 558
RseI CAYNNNNRTG 2 cut(s) 275, 953
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 4 cut(s) 99, 294, 395, 1125
Sau3AI GATC 8 cut(s) 21, 202, 658, 670, 817, 970, 1114, 1165
Sau96I GGNCC 4 cut(s) 412, 527, 717, 898
ScaI AGTACT 1 cut(s) 349
ScrFI CCNGG 4 cut(s) 73, 260, 668, 1098
SduI GDGCHC 2 cut(s) 9, 538
SfaNI GCATC 5 cut(s) 36, 82, 307, 755, 793
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 275, 953
SmlI CTYRAG 2 cut(s) 8, 293
SmoI CTYRAG 2 cut(s) 8, 293
SphI GCATGC 2 cut(s) 225, 229
Sse9I AATT 5 cut(s) 34, 150, 284, 427, 1049
SsiI CCGC 4 cut(s) 49, 111, 588, 624
SspMI CTAG 3 cut(s) 80, 464, 986
StyD4I CCNGG 4 cut(s) 71, 258, 666, 1096
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 4 cut(s) 56, 145, 352, 407
TaqI TCGA 3 cut(s) 9, 911, 943
TasI AATT 5 cut(s) 34, 150, 284, 427, 1049
TatI WGTACW 3 cut(s) 347, 547, 557
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 2 cut(s) 214, 1022
Tru1I TTAA 4 cut(s) 99, 294, 395, 1125
Tru9I TTAA 4 cut(s) 99, 294, 395, 1125
TscAI CASTG 5 cut(s) 205, 412, 577, 832, 937
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 5 cut(s) 269, 459, 503, 856, 933
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 5 cut(s) 205, 412, 577, 832, 937
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 1 cut(s) 150
XceI RCATGY 2 cut(s) 225, 229
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 3 cut(s) 80, 464, 986
ZrmI AGTACT 1 cut(s) 349
Zsp2I ATGCAT 2 cut(s) 227, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.