MD05G1119600.v1.1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001533
Physical Location & Seq
Forward (+)
24127957 .. 24131857
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1119600.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGTTCTCGAGGCGACTATGATCTGCGTCGACAATTCCGAATGGATGCGAAACGGCGATTACTCTCCCTCCCGATTGCAGGCTCAAGCTGACGCCATTAATCTCATCTGTGGTGCCAAAACCCAGGCTAATCCGGAGAATACAGTTGGGGTATTGACAATGGCGGGCAAAGGGGTGCGGGTTTTGGCTACTCCAACCTCTGATCTTGGCAGGATTTTGGCTTGCATGCATGGTCTGGAGATGGGAGGTGAGATGAACATAGCAGCTGCAATCCAGGTAGCACAGCTGGCTCTTAAGCATCGTCAAAACAAAAATCAACAACAGCGGATTATTGTTTTTGCTGGAAGTCCTGTTAAATTTGAAAAGAAGTTGTTGGAATCGATAGGGAAGAAATTGAAAAAGAACAGTGTTGCTCTTGACATTGTTGATTTTGGTGAAGAGGATGATGGGAAGCCAGAGAAGCTGGAGGCCCTTCTGTCTGCTGTTAATAATAATGAAAGCAGTCATATAGTACATGTCCCTCCTGGTCCAAATGCTCTCTCGGATGTTCTTATAAGTACACCTGTATTTACTGGTGACGGAGAAGGAGGAAGTGGTTTTGCAGTGGCGGCGGCAGCAGCAACTGCTGCTGCTAATGGTGGCTCTGGGTATGACTTTGGAGTTGATCCCAACATTGATCCTGAGCTGGCTCTCGCCCTTAGAGTTTCTATGGAAGAGGAAAGGGCTAGGCAAGAAGCTGCTGCCAAAAGAGCTTCTGAAGAAGCTGGTGGGAAAGGAGGGGAACCGTCATCCAAATCAGAAGATGCAACCATGACAGATCAAGCTAATGTTTCTTCTCCCAATGAGGATAAGAAACATACTGATAATATGGTTGATGAGAATGACTTGCTGAAGGAGGCTCTTGCAATGTCAATGAACGTCTCTGGAACTGGTCATTCAGCAGGTGATACTGAGATGTCCGAAGCAACTAGTGCGGATCAGGAGTTGGCATTAGCTCTTCAAATGTCGATGCAGGAAAGTGCAGGAGAGCCATCCTCCCAGAGCGATGCGAGCAAGGTGTTGGAGGATCAGTCTTTTATATCATCTATCCTTGAATCTCTTCCAGGAGTTGACCCCAACGATCCTTCAGTGAAAGATCTTCTTGCATCTCTGAAGAATCAGTCTGAGAAGGACAAAGAAGAACCATCGAACAAGGACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

401

Amino Acids

42.11

Weight (kDa)

4.58

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 3.3e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 6 cut(s) 164, 178, 325, 608, 611, 974
AclWI GGATC 5 cut(s) 659, 671, 984, 1074, 1115
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 4 cut(s) 777, 911, 1110, 1172
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 513, 559
AfiI CCNNNNNNNGG 1 cut(s) 79
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 4 cut(s) 362, 397, 1001, 1094
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 4 cut(s) 123, 273, 523, 1102
Alw26I GTCTC 1 cut(s) 925
AlwI GGATC 5 cut(s) 659, 671, 984, 1074, 1115
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 8 cut(s) 263, 266, 614, 617, 626, 629, 737, 740
ApoI RAATTY 1 cut(s) 356
AseI ATTAAT 1 cut(s) 99
Asp700I GAANNNNTTC 1 cut(s) 1098
AspS9I GGNCC 2 cut(s) 469, 527
AsuHPI GGTGA 4 cut(s) 260, 446, 587, 956
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BanI GGYRCC 1 cut(s) 113
BbvI GCAGC 8 cut(s) 253, 275, 613, 616, 626, 629, 724, 727
BccI CCATC 4 cut(s) 235, 440, 1039, 1192
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 4 cut(s) 125, 275, 525, 1104
BcoDI GTCTC 1 cut(s) 925
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 2 cut(s) 726, 969
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 932
BglII AGATCT 1 cut(s) 1135
Bme1390I CCNGG 4 cut(s) 125, 275, 525, 1104
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 469, 527
BmiI GGNNCC 2 cut(s) 115, 783
BmrFI CCNGG 4 cut(s) 125, 275, 525, 1104
BmsI GCATC 6 cut(s) 36, 307, 793, 999, 1036, 1154
BplI GAGNNNNNCTC 2 cut(s) 1019, 1051
BpmI CTGGAG 2 cut(s) 257, 485
Bpu10I CCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 1055, 1085
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 577, 934
Bse3DI GCAATG 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 4 cut(s) 125, 275, 525, 1104
BseCI ATCGAT 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 5 cut(s) 51, 448, 550, 788, 1031
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 3 cut(s) 672, 942, 1155
BseNI ACTGG 2 cut(s) 577, 934
BseXI GCAGC 8 cut(s) 253, 275, 613, 616, 626, 629, 724, 727
BsgI GTGCAG 1 cut(s) 1041
BshFI GGCC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 925
BsmBI CGTCTC 1 cut(s) 925
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
BspACI CCGC 6 cut(s) 164, 178, 325, 608, 611, 974
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 3 cut(s) 673, 943, 1156
BspDI ATCGAT 1 cut(s) 380
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 115, 783
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 5 cut(s) 659, 671, 984, 1074, 1115
BspQI GCTCTTC 1 cut(s) 1002
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 2 cut(s) 577, 934
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
BssNI GRCGYC 1 cut(s) 93
Bst2UI CCWGG 4 cut(s) 125, 275, 525, 1104
Bst4CI ACNGT 3 cut(s) 145, 407, 786
Bst6I CTCTTC 4 cut(s) 432, 708, 1002, 1104
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 3 cut(s) 623, 626, 971
BstC8I GCNNGC 7 cut(s) 81, 166, 223, 227, 288, 687, 1051
BstDEI CTNAG 4 cut(s) 681, 698, 951, 1164
BstF5I GGATG 5 cut(s) 51, 448, 550, 788, 1031
BstKTI GATC 9 cut(s) 24, 205, 667, 679, 820, 979, 1069, 1123, 1138
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
BstNI CCWGG 4 cut(s) 125, 275, 525, 1104
BstNSI RCATGY 2 cut(s) 229, 518
BstSCI CCNGG 4 cut(s) 123, 273, 523, 1102
BstV1I GCAGC 8 cut(s) 253, 275, 613, 616, 626, 629, 724, 727
BstX2I RGATCY 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 1135
Bsu15I ATCGAT 1 cut(s) 380
BsuRI GGCC 1 cut(s) 470
BsuTUI ATCGAT 1 cut(s) 380
BtgZI GCGATG 1 cut(s) 1059
BtsCI GGATG 5 cut(s) 51, 448, 550, 788, 1031
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 412, 609, 1134
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 7 cut(s) 81, 166, 223, 227, 288, 687, 1051
CaiI CAGNNNCTG 1 cut(s) 623
Cfr13I GGNCC 2 cut(s) 469, 527
ClaI ATCGAT 1 cut(s) 380
CseI GACGC 2 cut(s) 16, 101
Csp6I GTAC 2 cut(s) 512, 558
CviAII CATG 4 cut(s) 226, 230, 515, 811
CviQI GTAC 2 cut(s) 512, 558
DdeI CTNAG 4 cut(s) 681, 698, 951, 1164
DpnI GATC 9 cut(s) 23, 204, 666, 678, 819, 978, 1068, 1122, 1137
DpnII GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
Eam1104I CTCTTC 4 cut(s) 432, 708, 1002, 1104
EarI CTCTTC 4 cut(s) 432, 708, 1002, 1104
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 4 cut(s) 777, 911, 1110, 1172
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 4 cut(s) 123, 273, 523, 1102
EcoT22I ATGCAT 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 925
FaeI CATG 4 cut(s) 229, 233, 518, 814
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 225, 229, 514, 810
FauI CCCGC 2 cut(s) 157, 171
FblI GTMKAC 1 cut(s) 30
FokI GGATG 5 cut(s) 58, 455, 557, 775, 1018
FspBI CTAG 2 cut(s) 726, 969
GsuI CTGGAG 2 cut(s) 257, 485
HaeIII GGCC 1 cut(s) 470
HapII CCGG 1 cut(s) 134
HgaI GACGC 2 cut(s) 16, 101
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 4 cut(s) 229, 233, 518, 814
HincII GTYRAC 2 cut(s) 31, 1111
HindII GTYRAC 2 cut(s) 31, 1111
HinfI GANTC 3 cut(s) 377, 1094, 1156
HpaII CCGG 1 cut(s) 134
HphI GGTGA 4 cut(s) 260, 446, 587, 956
Hpy166II GTNNAC 3 cut(s) 31, 560, 1111
Hpy188I TCNGA 8 cut(s) 40, 202, 544, 757, 799, 961, 1152, 1165
Hpy188III TCNNGA 8 cut(s) 8, 72, 134, 236, 416, 680, 924, 980
Hpy8I GTNNAC 3 cut(s) 31, 560, 1111
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 5 cut(s) 482, 578, 886, 1134, 1162
HpyCH4III ACNGT 3 cut(s) 145, 407, 786
HpyCH4IV ACGT 1 cut(s) 918
HpyF3I CTNAG 4 cut(s) 681, 698, 951, 1164
HpySE526I ACGT 1 cut(s) 918
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 4 cut(s) 229, 233, 518, 814
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
LguI GCTCTTC 1 cut(s) 1002
Lsp1109I GCAGC 8 cut(s) 253, 275, 613, 616, 626, 629, 724, 727
LweI GCATC 6 cut(s) 36, 307, 793, 999, 1036, 1154
MaeI CTAG 2 cut(s) 726, 969
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 1 cut(s) 575
MalI GATC 9 cut(s) 23, 204, 666, 678, 819, 978, 1068, 1122, 1137
MboI GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
MflI RGATCY 1 cut(s) 1135
MluCI AATT 3 cut(s) 34, 356, 392
MmeI TCCRAC 3 cut(s) 218, 354, 1041
Mph1103I ATGCAT 1 cut(s) 231
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 1098
MseI TTAA 4 cut(s) 99, 294, 354, 486
MspA1I CMGCKG 3 cut(s) 266, 286, 325
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 4 cut(s) 125, 275, 525, 1104
MvaI CCWGG 4 cut(s) 125, 275, 525, 1104
NdeII GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
NlaIII CATG 4 cut(s) 229, 233, 518, 814
NlaIV GGNNCC 2 cut(s) 115, 783
NmuCI GTSAC 1 cut(s) 575
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 2 cut(s) 229, 518
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 932
PciI ACATGT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 1098
PfeI GAWTC 3 cut(s) 377, 1094, 1156
PfoI TCCNGGA 1 cut(s) 1102
PscI ACATGT 1 cut(s) 514
PshBI ATTAAT 1 cut(s) 99
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 4 cut(s) 123, 273, 523, 1102
PspGI CCWGG 4 cut(s) 123, 273, 523, 1102
PspN4I GGNNCC 2 cut(s) 115, 783
PspPI GGNCC 2 cut(s) 469, 527
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 1 cut(s) 1135
PvuII CAGCTG 2 cut(s) 266, 286
RsaI GTAC 2 cut(s) 513, 559
RsaNI GTAC 2 cut(s) 512, 558
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 99, 294, 354, 486
Sau3AI GATC 9 cut(s) 21, 202, 664, 676, 817, 976, 1066, 1120, 1135
Sau96I GGNCC 2 cut(s) 469, 527
ScrFI CCNGG 4 cut(s) 125, 275, 525, 1104
SfaNI GCATC 6 cut(s) 36, 307, 793, 999, 1036, 1154
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 84, 293
SmoI CTYRAG 3 cut(s) 8, 84, 293
SpeI ACTAGT 1 cut(s) 968
SphI GCATGC 1 cut(s) 229
Sse9I AATT 3 cut(s) 34, 356, 392
SsiI CCGC 6 cut(s) 164, 178, 325, 608, 611, 974
SspMI CTAG 2 cut(s) 726, 969
StyD4I CCNGG 4 cut(s) 123, 273, 523, 1102
TaaI ACNGT 3 cut(s) 145, 407, 786
TaiI ACGT 1 cut(s) 921
TaqI TCGA 5 cut(s) 9, 30, 380, 1007, 1187
TasI AATT 3 cut(s) 34, 356, 392
TatI WGTACW 2 cut(s) 511, 557
TauI GCSGC 2 cut(s) 611, 614
TfiI GAWTC 3 cut(s) 377, 1094, 1156
Tru1I TTAA 4 cut(s) 99, 294, 354, 486
Tru9I TTAA 4 cut(s) 99, 294, 354, 486
TscAI CASTG 3 cut(s) 412, 609, 1134
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 8 cut(s) 263, 266, 614, 617, 626, 629, 737, 740
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 3 cut(s) 269, 510, 929
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 3 cut(s) 412, 609, 1134
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 99
XapI RAATTY 1 cut(s) 356
XceI RCATGY 2 cut(s) 229, 518
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 2 cut(s) 726, 969
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.