pycom02g10090

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001533
Physical Location & Seq
Reverse (-)
6974707 .. 6978694
3988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10090.11

Sequence Viewer

Length: 1212 bp
ATGGTGCTCGAGGCGACAATGATCTGCATTGATAATTCGGAATGGATGCGGAACGGCGATTACTCGCCGTCGAGGTTTCAAGCTCAGGCCGATGCTGTCAATCTGATCTGCGGAGCTAAAACCCAGTCTAATCCGGAGAATACAGTAGGAGTTCTCACAATGGCAGGAAAAGGTGTTCGCGTTTTGGTCACTCCAACCAGTGATCTAGGCAAGATTCTTGCATGCATGCATGGTTTAGAAATCGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCTCTCAAGCATCGCCAAAATAAGAAACAGCAACAGAGGATTATCGTGTTTGCTGGAAGTCCTGTCAAACATGAGAAAAAGCTTTTAGAGATGATTGGAAGGAAGTTAAAGAAGAACAGTGTAGCCCTTGATATTGTCAATTTTGGTGAAGAAGATGAAGATAAGTCAGAGAAGCTAGAAGCACTACTTGCTGCTGTGAACAATAATGATACCAGTCACATTGTTCATGTACCAGCAGGTCCAAGTGCTCTTTCTGATGTACTTATAAGTACACCCATCTTCACTGGGGATGGGGAAGGTGGGAGTGGTTTTGCAGCGGCAGCTGCTGCTGCTGCTGCGGGTGGTGTCTCTGGTTTTGATTTTGGCGTGGATCCAAACCTGGATCCTGAGCTTGCCCTTGCTCTTAGAGTTTCAATGGAAGAGGAGAGGGCCAGACAAGAAGCAGCTGCTAAAAAAGCAGCTGAGGATGCTGGCAAACAGGAAAAAGGAGTGGAGGGGCAATCCAGCTCACAAGATGCAACGATGACGGAGCGTGCCAGTGTTGGGACTTCTGAAGCTGAGAATAAGAGTAGTGATTTCATGGATGATGAGAATGCTCTGCTACAACAGGCCCTTGCGATGTCGATGGATGAACCTGCTTCCAGCCATGATATTCGAGACACGGATATGTCCGAGGCAACTGATCCAGAGTTGGCTCTAGCTCTTCAATTGTCTGTTCAGGAAGGTGAAAAAGATTCTGGTAGCGAGAAAGATATGAGTAAATTATTAGCCGACCAGTCTTTCGTGTCTTCCATCCTTGCCTCGCTTCCAGGGGTTGACCCAAATGATCCTTCAGTTAAAGATTTGCTTGCTTCCATGCAAAACCAATCAGAGCCACATCTACAGAAGAACGAAGACAAACCACCAAAAGAGGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

404

Amino Acids

42.8

Weight (kDa)

4.61

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
Acc36I ACCTGC 2 cut(s) 515, 931
AccII CGCG 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 4 cut(s) 49, 111, 605, 626
AclWI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
AcuI CTGAAG 2 cut(s) 861, 1104
AfaI GTAC 3 cut(s) 519, 549, 559
AfiI CCNNNNNNNGG 1 cut(s) 831
AgsI TTSAA 3 cut(s) 80, 702, 995
AjnI CCWGG 3 cut(s) 258, 666, 1096
AjuI GAANNNNNNNTTGG 4 cut(s) 523, 555, 1102, 1134
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw21I GWGCWC 2 cut(s) 9, 538
Alw26I GTCTC 2 cut(s) 640, 939
AlwI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 3 cut(s) 87, 717, 897
ArsI GACNNNNNNTTYG 2 cut(s) 1052, 1084
AspS9I GGNCC 3 cut(s) 527, 717, 898
AsuHPI GGTGA 3 cut(s) 260, 446, 1025
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BamHI GGATCC 2 cut(s) 658, 670
BbsI GAAGAC 2 cut(s) 1068, 1188
Bbv12I GWGCWC 2 cut(s) 9, 538
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 4 cut(s) 572, 572, 907, 1088
BceAI ACGGC 2 cut(s) 52, 70
BciT130I CCWGG 3 cut(s) 260, 668, 1098
BcoDI GTCTC 2 cut(s) 640, 939
BfaI CTAG 3 cut(s) 206, 464, 986
BfmI CTRYAG 1 cut(s) 1169
BfuAI ACCTGC 2 cut(s) 515, 931
Bme1390I CCNGG 3 cut(s) 260, 668, 1098
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 527, 717, 898
BmiI GGNNCC 2 cut(s) 660, 672
BmrFI CCNGG 3 cut(s) 260, 668, 1098
BmrI ACTGGG 2 cut(s) 118, 582
BmsI GCATC 5 cut(s) 36, 82, 307, 745, 793
BmuI ACTGGG 2 cut(s) 118, 582
BpiI GAAGAC 2 cut(s) 1068, 1188
Bpu10I CCTNAGC 3 cut(s) 84, 675, 750
BpuEI CTTGAG 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 960, 1097
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 831
Bse1I ACTGG 6 cut(s) 124, 198, 501, 577, 825, 1063
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 3 cut(s) 260, 668, 1098
BseDI CCNNGG 2 cut(s) 960, 1097
BseGI GGATG 6 cut(s) 51, 583, 760, 877, 922, 1080
BseLI CCNNNNNNNGG 1 cut(s) 831
BseMII CTCAG 4 cut(s) 98, 666, 741, 837
BseNI ACTGG 6 cut(s) 124, 198, 501, 577, 825, 1063
BseRI GAGGAG 1 cut(s) 725
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 89, 719, 899
BsiHKAI GWGCWC 2 cut(s) 9, 538
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 847
BslI CCNNNNNNNGG 1 cut(s) 831
BsmAI GTCTC 2 cut(s) 640, 939
BsmFI GGGAC 1 cut(s) 847
BsmI GAATGC 2 cut(s) 270, 886
BsnI GGCC 3 cut(s) 89, 719, 899
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 9, 538
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
BspACI CCGC 4 cut(s) 49, 111, 605, 626
BspANI GGCC 3 cut(s) 89, 719, 899
BspCNI CTCAG 4 cut(s) 97, 667, 742, 838
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 660, 672
BspMI ACCTGC 2 cut(s) 515, 931
BspPI GGATC 6 cut(s) 653, 665, 666, 678, 965, 1109
BspQI GCTCTTC 1 cut(s) 996
BsrI ACTGG 6 cut(s) 124, 198, 501, 577, 825, 1063
BssECI CCNNGG 2 cut(s) 960, 1097
BssMI GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
Bst2UI CCWGG 3 cut(s) 260, 668, 1098
Bst4CI ACNGT 2 cut(s) 145, 407
Bst6I CTCTTC 2 cut(s) 702, 996
BstAPI GCANNNNNTGC 2 cut(s) 476, 614
BstC8I GCNNGC 7 cut(s) 223, 227, 268, 681, 760, 822, 1137
BstDEI CTNAG 5 cut(s) 84, 675, 692, 750, 846
BstF5I GGATG 6 cut(s) 51, 583, 760, 877, 922, 1080
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 7 cut(s) 24, 108, 205, 661, 673, 973, 1117
BstMAI GTCTC 2 cut(s) 640, 939
BstMBI GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
BstNI CCWGG 3 cut(s) 260, 668, 1098
BstNSI RCATGY 2 cut(s) 225, 229
BstSCI CCNGG 3 cut(s) 258, 666, 1096
BstSFI CTRYAG 1 cut(s) 1169
BstUI CGCG 1 cut(s) 180
BstV2I GAAGAC 2 cut(s) 1068, 1188
BstX2I RGATCY 2 cut(s) 658, 670
BstYI RGATCY 2 cut(s) 658, 670
BsuRI GGCC 3 cut(s) 89, 719, 899
BtgZI GCGATG 2 cut(s) 284, 920
BtsCI GGATG 6 cut(s) 51, 583, 760, 877, 922, 1080
BtsIMutI CAGTG 4 cut(s) 205, 412, 570, 832
BveI ACCTGC 2 cut(s) 515, 931
Cac8I GCNNGC 7 cut(s) 223, 227, 268, 681, 760, 822, 1137
CaiI CAGNNNCTG 1 cut(s) 614
Cfr13I GGNCC 3 cut(s) 527, 717, 898
Csp6I GTAC 3 cut(s) 518, 548, 558
CviAII CATG 8 cut(s) 222, 226, 230, 359, 515, 868, 935, 1144
CviQI GTAC 3 cut(s) 518, 548, 558
DdeI CTNAG 5 cut(s) 84, 675, 692, 750, 846
DpnI GATC 7 cut(s) 23, 107, 204, 660, 672, 972, 1116
DpnII GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
Eam1104I CTCTTC 2 cut(s) 702, 996
EarI CTCTTC 2 cut(s) 702, 996
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 2 cut(s) 861, 1104
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 258, 666, 1096
EcoT22I ATGCAT 2 cut(s) 227, 231
FaeI CATG 8 cut(s) 225, 229, 233, 362, 518, 871, 938, 1147
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 1119, 1151
FaqI GGGAC 1 cut(s) 847
FatI CATG 8 cut(s) 221, 225, 229, 358, 514, 867, 934, 1143
FauI CCCGC 1 cut(s) 619
FokI GGATG 6 cut(s) 58, 590, 767, 884, 929, 1067
FspBI CTAG 3 cut(s) 206, 464, 986
HaeIII GGCC 3 cut(s) 89, 719, 899
HapII CCGG 1 cut(s) 134
Hin1II CATG 8 cut(s) 225, 229, 233, 362, 518, 871, 938, 1147
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 214, 1022
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 260, 446, 1025
Hpy166II GTNNAC 3 cut(s) 487, 560, 1105
Hpy188I TCNGA 7 cut(s) 40, 105, 457, 544, 841, 961, 1159
Hpy188III TCNNGA 5 cut(s) 134, 674, 944, 974, 1007
Hpy8I GTNNAC 3 cut(s) 487, 560, 1105
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 381, 578, 1004, 1128
HpyCH4III ACNGT 2 cut(s) 145, 407
HpyCH4V TGCA 7 cut(s) 27, 221, 225, 229, 602, 806, 1147
HpyF3I CTNAG 5 cut(s) 84, 675, 692, 750, 846
Hsp92II CATG 8 cut(s) 225, 229, 233, 362, 518, 871, 938, 1147
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 2 cut(s) 113, 817
LweI GCATC 5 cut(s) 36, 82, 307, 745, 793
MaeI CTAG 3 cut(s) 206, 464, 986
MaeIII GTNAC 2 cut(s) 187, 503
MalI GATC 7 cut(s) 23, 107, 204, 660, 672, 972, 1116
MboI GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
MfeI CAATTG 2 cut(s) 284, 995
MflI RGATCY 2 cut(s) 658, 670
MhlI GDGCHC 2 cut(s) 9, 538
MluCI AATT 5 cut(s) 34, 284, 427, 995, 1049
MmeI TCCRAC 1 cut(s) 218
Mph1103I ATGCAT 2 cut(s) 227, 231
MroI TCCGGA 1 cut(s) 133
MseI TTAA 2 cut(s) 395, 1125
MslI CAYNNNNRTG 2 cut(s) 275, 953
MspA1I CMGCKG 4 cut(s) 605, 611, 734, 749
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 3 cut(s) 260, 668, 1098
MunI CAATTG 2 cut(s) 284, 995
Mva1269I GAATGC 2 cut(s) 270, 886
MvaI CCWGG 3 cut(s) 260, 668, 1098
MvnI CGCG 1 cut(s) 180
NdeII GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
NlaIII CATG 8 cut(s) 225, 229, 233, 362, 518, 871, 938, 1147
NlaIV GGNNCC 2 cut(s) 660, 672
NmuCI GTSAC 2 cut(s) 187, 503
NsiI ATGCAT 2 cut(s) 227, 231
NspI RCATGY 2 cut(s) 225, 229
PaeI GCATGC 2 cut(s) 225, 229
PaeR7I CTCGAG 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 2 cut(s) 270, 886
PfeI GAWTC 2 cut(s) 214, 1022
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 3 cut(s) 258, 666, 1096
PspGI CCWGG 3 cut(s) 258, 666, 1096
PspN4I GGNNCC 2 cut(s) 660, 672
PspPI GGNCC 3 cut(s) 527, 717, 898
PspXI VCTCGAGB 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 44, 76
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 2 cut(s) 658, 670
PvuII CAGCTG 3 cut(s) 611, 734, 749
RsaI GTAC 3 cut(s) 519, 549, 559
RsaNI GTAC 3 cut(s) 518, 548, 558
RseI CAYNNNNRTG 2 cut(s) 275, 953
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 2 cut(s) 395, 1125
Sau3AI GATC 7 cut(s) 21, 105, 202, 658, 670, 970, 1114
Sau96I GGNCC 3 cut(s) 527, 717, 898
ScrFI CCNGG 3 cut(s) 260, 668, 1098
SduI GDGCHC 2 cut(s) 9, 538
SfaNI GCATC 5 cut(s) 36, 82, 307, 745, 793
SfcI CTRYAG 1 cut(s) 1169
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 275, 953
SmlI CTYRAG 2 cut(s) 8, 293
SmoI CTYRAG 2 cut(s) 8, 293
SphI GCATGC 2 cut(s) 225, 229
Sse9I AATT 5 cut(s) 34, 284, 427, 995, 1049
SsiI CCGC 4 cut(s) 49, 111, 605, 626
SspMI CTAG 3 cut(s) 206, 464, 986
StyD4I CCNGG 3 cut(s) 258, 666, 1096
TaaI ACNGT 2 cut(s) 145, 407
TaqI TCGA 4 cut(s) 9, 71, 911, 943
TasI AATT 5 cut(s) 34, 284, 427, 995, 1049
TatI WGTACW 2 cut(s) 547, 557
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 2 cut(s) 214, 1022
Tru1I TTAA 2 cut(s) 395, 1125
Tru9I TTAA 2 cut(s) 395, 1125
TscAI CASTG 4 cut(s) 205, 412, 577, 832
TseFI GTSAC 2 cut(s) 187, 503
Tsp45I GTSAC 2 cut(s) 187, 503
TspDTI ATGAA 5 cut(s) 269, 459, 503, 856, 933
TspGWI ACGGA 2 cut(s) 830, 965
TspRI CASTG 4 cut(s) 205, 412, 577, 832
VpaK11BI GGWCC 1 cut(s) 527
XceI RCATGY 2 cut(s) 225, 229
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 3 cut(s) 206, 464, 986
Zsp2I ATGCAT 2 cut(s) 227, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.