FvH4_2g26060

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001533
Physical Location & Seq
Reverse (-)
20982196 .. 20983398
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26060.t1

Sequence Viewer

Length: 1203 bp
ATGGTTCTCGAGGCGACAATGATTTGCATCGACAATTCAGAATATATGCGAAACGAAGATTACTCTCCCTCCCGATGCTTAGCTCAGGCCGATGCAGTAAATCTCATCTGTGGTATGAAAACCCAGGACAACCCGGAAAACACAGTTGGGGTGTTGACAATGGCAGGCAAAGCGGTTCGCGTACTCACCACGCCCACCTCTGATATTGGCAAGATTTTGACATGCACGCATGGCCTCGAAATTGGTGGTGAGATGAACATAGCAGGTGCAATCCAGGTAGCTCAGTTGGCTCTTAAGCATCGCCAAAACAAAAACCAACAACAAAGGATTGTAGTTTTTGCTGGAAGTACAGTCAAGAATGACAAAAAGGCATTGGAAATAATTGGGAAGAAACTGAAGAAAAATAGTGTTGCTCTTGACATTGTCGATTTTGGAGGAGAAGATGAAGAAAAGTCGGAGAAGCTGGATGCCCTTGTTTCTGCTGTTGATAATGGCACTAGTCACATAGTCCATATCCCTCATGGTCAAAATCTTTCTGATGTTATTCTAAGTTCACCTATATTAACAAGTAACGAAGGAGGAGCTGGCTTTGCAGCCGCAGCAGCTGGTATTGGTGGTTCTTCTGGCCTTGAGTTTGGAGTAGATCCTAATATAGATCCCGAGCTTGCTCTTGCCCTTCGAATGTCAATGGAAGAGGAAAGGGCAAGACAAGAGGCTGCTGCAGCCAGAAGAGCGGAGGAAGAAGCTGCTACACAAGGGAAAGAAGAGGAACGACAATCCAAATCGGAGGATGTGGCCATGACAGAAAGAGATGATGTTGAAATGGTTGATGAGGATGCCTTGCTAGTGCAAGCTCTTGCGATGTCAATGAATAACTATGGCTCTGATAGTTCTGCAGTAGGTGATGCTGCGATGTCAGAGGCAACTGGTGTGGACGAGGATTTGGCTCTGGCTCTTCCAATGTCCATACAGGAAAGCGCAAAAGGGGCATCATCATCCGAGGCAGCTGATGTGAGCAAGGTGTTGGAGGATCAGTCTTTTATTTCGTCTATCCTGGCATCCCTTCCAGGAGTTGACTCCAATGATCCTTCTGTGAAGGAGTTTTTTGCATCTCTGAATAATCAGTCCAAAGAGTTTCTTGCATCTCTGAATAATCAGTCCAAAAAGGATGAACAAGATCCATCTAATGACCAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

401

Amino Acids

42.48

Weight (kDa)

4.49

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 2.6e-29 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 734
AccII CGCG 1 cut(s) 180
AciI CCGC 3 cut(s) 173, 597, 734
AclWI GGATC 5 cut(s) 638, 650, 1038, 1079, 1172
AcoI YGGCCR 1 cut(s) 795
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 2 cut(s) 183, 349
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 1 cut(s) 821
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 4 cut(s) 123, 273, 1053, 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 9 cut(s) 83, 281, 463, 584, 605, 664, 746, 854, 1007
AluI AGCT 9 cut(s) 83, 281, 463, 584, 605, 664, 746, 854, 1007
AlwI GGATC 5 cut(s) 638, 650, 1038, 1079, 1172
AlwNI CAGNNNCTG 1 cut(s) 605
Ama87I CYCGRG 2 cut(s) 8, 659
AoxI GGCC 4 cut(s) 87, 232, 625, 795
ApeKI GCWGC 9 cut(s) 593, 599, 602, 716, 719, 722, 746, 908, 1004
AspLEI GCGC 1 cut(s) 980
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 4 cut(s) 178, 260, 546, 914
AsuII TTCGAA 1 cut(s) 679
AvaI CYCGRG 2 cut(s) 8, 659
BalI TGGCCA 1 cut(s) 797
BbvI GCAGC 9 cut(s) 605, 611, 614, 703, 706, 733, 734, 895, 1016
BccI CCATC 1 cut(s) 1189
BciT130I CCWGG 4 cut(s) 125, 275, 1055, 1068
BcnI CCSGG 1 cut(s) 134
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 2 cut(s) 498, 845
BfmI CTRYAG 2 cut(s) 720, 894
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 254
BlpI GCTNAGC 1 cut(s) 79
Bme1390I CCNGG 5 cut(s) 125, 134, 275, 1055, 1068
BmeT110I CYCGRG 2 cut(s) 8, 659
BmrFI CCNGG 5 cut(s) 125, 134, 275, 1055, 1068
Bpu10I CCTNAGC 1 cut(s) 84
Bpu1102I GCTNAGC 1 cut(s) 79
Bpu14I TTCGAA 1 cut(s) 679
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 134
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 123, 999
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 1019, 1049
Bse1I ACTGG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 4 cut(s) 125, 275, 1055, 1068
BseDI CCNNGG 2 cut(s) 123, 999
BseGI GGATG 6 cut(s) 472, 796, 841, 995, 1058, 1174
BseJI GATNNNNATC 1 cut(s) 26
BseMII CTCAG 2 cut(s) 98, 296
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 2 cut(s) 450, 594
BseXI GCAGC 9 cut(s) 605, 611, 614, 703, 706, 733, 734, 895, 1016
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 89, 234, 627, 797
BsiHKCI CYCGRG 2 cut(s) 8, 659
BsiSI CCGG 1 cut(s) 134
BsnI GGCC 4 cut(s) 89, 234, 627, 797
BsoBI CYCGRG 2 cut(s) 8, 659
Bsp119I TTCGAA 1 cut(s) 679
Bsp143I GATC 5 cut(s) 643, 655, 1030, 1084, 1177
Bsp1720I GCTNAGC 1 cut(s) 79
BspACI CCGC 3 cut(s) 173, 597, 734
BspANI GGCC 4 cut(s) 89, 234, 627, 797
BspCNI CTCAG 2 cut(s) 97, 295
BspFNI CGCG 1 cut(s) 180
BspMAI CTGCAG 2 cut(s) 724, 898
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 5 cut(s) 638, 650, 1038, 1079, 1172
BspQI GCTCTTC 2 cut(s) 724, 960
BspT104I TTCGAA 1 cut(s) 679
BspTI CTTAAG 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 734
BsrI ACTGG 1 cut(s) 931
BssECI CCNNGG 2 cut(s) 123, 999
BssMI GATC 5 cut(s) 643, 655, 1030, 1084, 1177
Bst2UI CCWGG 4 cut(s) 125, 275, 1055, 1068
Bst4CI ACNGT 2 cut(s) 145, 352
Bst6I CTCTTC 4 cut(s) 687, 724, 759, 960
BstAFI CTTAAG 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 679
BstC8I GCNNGC 5 cut(s) 166, 227, 586, 666, 852
BstDEI CTNAG 4 cut(s) 79, 84, 282, 548
BstF5I GGATG 6 cut(s) 472, 796, 841, 995, 1058, 1174
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 980
BstKTI GATC 5 cut(s) 646, 658, 1033, 1087, 1180
BstMBI GATC 5 cut(s) 643, 655, 1030, 1084, 1177
BstMWI GCNNNNNNNGC 9 cut(s) 170, 231, 287, 590, 599, 602, 722, 731, 986
BstNI CCWGG 4 cut(s) 125, 275, 1055, 1068
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 5 cut(s) 123, 132, 273, 1053, 1066
BstSFI CTRYAG 2 cut(s) 720, 894
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 9 cut(s) 605, 611, 614, 703, 706, 733, 734, 895, 1016
BstX2I RGATCY 3 cut(s) 643, 655, 1177
BstYI RGATCY 3 cut(s) 643, 655, 1177
BsuRI GGCC 4 cut(s) 89, 234, 627, 797
BtgZI GCGATG 3 cut(s) 284, 875, 926
BtsCI GGATG 6 cut(s) 472, 796, 841, 995, 1058, 1174
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 5 cut(s) 166, 227, 586, 666, 852
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 1 cut(s) 980
Csp6I GTAC 2 cut(s) 182, 348
CspCI CAANNNNNGTGG 2 cut(s) 912, 947
CviAII CATG 4 cut(s) 222, 230, 521, 799
CviQI GTAC 2 cut(s) 182, 348
DdeI CTNAG 4 cut(s) 79, 84, 282, 548
DpnI GATC 5 cut(s) 645, 657, 1032, 1086, 1179
DpnII GATC 5 cut(s) 643, 655, 1030, 1084, 1177
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 4 cut(s) 687, 724, 759, 960
EarI CTCTTC 4 cut(s) 687, 724, 759, 960
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 2 cut(s) 8, 659
EcoRII CCWGG 4 cut(s) 123, 273, 1053, 1066
FaeI CATG 4 cut(s) 225, 233, 524, 802
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FatI CATG 4 cut(s) 221, 229, 520, 798
FokI GGATG 6 cut(s) 479, 803, 848, 982, 1045, 1181
FspBI CTAG 2 cut(s) 498, 845
GlaI GCGC 1 cut(s) 979
HaeIII GGCC 4 cut(s) 89, 234, 627, 797
HapII CCGG 1 cut(s) 134
HhaI GCGC 1 cut(s) 980
Hin1II CATG 4 cut(s) 225, 233, 524, 802
Hin6I GCGC 1 cut(s) 978
HinP1I GCGC 1 cut(s) 978
HincII GTYRAC 2 cut(s) 156, 1075
HindII GTYRAC 2 cut(s) 156, 1075
HinfI GANTC 1 cut(s) 1076
HpaII CCGG 1 cut(s) 134
HphI GGTGA 4 cut(s) 178, 260, 546, 914
Hpy166II GTNNAC 4 cut(s) 156, 554, 934, 1075
Hpy188III TCNNGA 5 cut(s) 8, 72, 355, 416, 659
Hpy8I GTNNAC 4 cut(s) 156, 554, 934, 1075
HpyAV CCTTC 5 cut(s) 569, 686, 1073, 1090, 1098
HpyCH4III ACNGT 2 cut(s) 145, 352
HpyF10VI GCNNNNNNNGC 9 cut(s) 170, 231, 287, 590, 599, 602, 722, 731, 986
HpyF3I CTNAG 4 cut(s) 79, 84, 282, 548
Hsp92II CATG 4 cut(s) 225, 233, 524, 802
HspAI GCGC 1 cut(s) 978
Kzo9I GATC 5 cut(s) 643, 655, 1030, 1084, 1177
LguI GCTCTTC 2 cut(s) 724, 960
LmnI GCTCC 1 cut(s) 581
Lsp1109I GCAGC 9 cut(s) 605, 611, 614, 703, 706, 733, 734, 895, 1016
MaeI CTAG 2 cut(s) 498, 845
MaeIII GTNAC 2 cut(s) 500, 569
MalI GATC 5 cut(s) 645, 657, 1032, 1086, 1179
MbiI CCGCTC 1 cut(s) 734
MboI GATC 5 cut(s) 643, 655, 1030, 1084, 1177
MflI RGATCY 3 cut(s) 643, 655, 1177
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 3 cut(s) 34, 240, 381
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 2 cut(s) 435, 1005
Mox20I TGGCCA 1 cut(s) 797
MscI TGGCCA 1 cut(s) 797
MseI TTAA 2 cut(s) 294, 563
Msp20I TGGCCA 1 cut(s) 797
MspA1I CMGCKG 2 cut(s) 605, 1007
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 5 cut(s) 125, 134, 275, 1055, 1068
MvaI CCWGG 4 cut(s) 125, 275, 1055, 1068
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 170, 231, 287, 590, 599, 602, 722, 731, 986
NciI CCSGG 1 cut(s) 134
NdeII GATC 5 cut(s) 643, 655, 1030, 1084, 1177
NlaIII CATG 4 cut(s) 225, 233, 524, 802
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 225
NspV TTCGAA 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 254
PciSI GCTCTTC 2 cut(s) 724, 960
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 1066
PleI GAGTC 1 cut(s) 1070
PpsI GAGTC 1 cut(s) 1070
Psp6I CCWGG 4 cut(s) 123, 273, 1053, 1066
PspGI CCWGG 4 cut(s) 123, 273, 1053, 1066
PsrI GAACNNNNNNTAC 2 cut(s) 601, 633
PstI CTGCAG 2 cut(s) 724, 898
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 3 cut(s) 643, 655, 1177
PsyI GACNNNGTC 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 605, 1007
RsaI GTAC 2 cut(s) 183, 349
RsaNI GTAC 2 cut(s) 182, 348
SapI GCTCTTC 2 cut(s) 724, 960
SaqAI TTAA 2 cut(s) 294, 563
Sau3AI GATC 5 cut(s) 643, 655, 1030, 1084, 1177
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 5 cut(s) 125, 134, 275, 1055, 1068
SfcI CTRYAG 2 cut(s) 720, 894
Sfr274I CTCGAG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 679
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 293, 629
SmoI CTYRAG 3 cut(s) 8, 293, 629
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 3 cut(s) 34, 240, 381
SsiI CCGC 3 cut(s) 173, 597, 734
SspMI CTAG 2 cut(s) 498, 845
StyD4I CCNGG 5 cut(s) 123, 132, 273, 1053, 1066
TaaI ACNGT 2 cut(s) 145, 352
TaqI TCGA 5 cut(s) 9, 30, 237, 426, 679
TasI AATT 3 cut(s) 34, 240, 381
TatI WGTACW 1 cut(s) 347
TauI GCSGC 1 cut(s) 599
Tru1I TTAA 2 cut(s) 294, 563
Tru9I TTAA 2 cut(s) 294, 563
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 9 cut(s) 593, 599, 602, 716, 719, 722, 746, 908, 1004
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 5 cut(s) 131, 269, 459, 884, 1185
Tth111I GACNNNGTC 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 293
XceI RCATGY 1 cut(s) 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 2 cut(s) 498, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.