pycom15g21460

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001533
Physical Location & Seq
Reverse (-)
15411415 .. 15415317
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21460.1

Sequence Viewer

Length: 1224 bp
ATGGTGCTCGAGGCGACAATGATCTGCATTGATAATTCGGAATGGATGCGGAACGGCGATTACGCTCCGTCCAGGTTTCAAGCTCAGGCCGATGCTGTTAATCTGATTTGCGGCGCTAAAACCCACTCTAATCCGGAGAATACAGTAGGAATTCTCACCATGGCAGGAAAAGGTGTTCGTGTTTTGGTCACTCCAACCAGTGATCTTGGCAAGATTCTGGCATGCATGCATGGTTTAGAAATAGGTGGTGAGATGAACCTGGCTGCTGGTATTCAGGTGGCTCAATTGGCTCTCAAGCATCGCCAAAATAAGAAACAGCAACAGAGAATTATTGTGTTCGCTGGAAGTACTGTCAAACATGACAAAACACTTTTAGAGATGATTGGAAGGAAGTTAAAGAAGAACAGTGTGGCCCTTGATATTGTCAATTTTGGTGAAGAAGATGAAGCTAAGACAGAGAAGCTAGAAGCACTACTTGCTGCTGTGAACAATAATGACACCAGCCACATTGTTCATGTACCAGCAGGTCCAAGTGCTCTTTCTGATGTACTTATAAGTACACCTATCTTCACCGGAGATGGGGAAGGCGGGAGTGGTTTTGCCGCAGCAGCAGCAGCTGCTGCCGCAGGTGGTGTCTCTGGTTTTGATTTCGGCGTGGATCCAAACCTGGATCCTGAGCTTGCCCTTGCTCTTAGAGTTTCAATGGAAGAAGAGAGGGCCAGACAAGAAGCAGCTGCTAAAAAGGCATCCGAGGATGCTGCCAAACAGGAAAAAGGAGGGGAGGGGCAATCCAGCTCACAAGATGCAACGATGACTGATCGTGCCAGTGTTGGAACTTCTGAAGCTGAGAATAAGAGTAGTGATTTCATGGATGATGAGAATGCCCTGCTACAACAGGCCCTTGCAATGTCGATGGATGAACCTGCCTCCAGCGATGATATTCGAGACACAGATATGTCCAAGGCAACTGATCCAGAGTTGGCTCTTGCTCTTCAAATGTCTGTTCAGGAAGGGGGAAAAGATTCTGGTAGCGAGAAGGATATGAGTAAATTACTGGCCGATCAGTCTTTTGTGTCTTCCATCCTTGCCTCGCTTCCAGGGGTTGACCCAAATCATCATTTCGAGGATCCTTCAGTTAAAGATTTGCTTGCTTCAATGCAAAACCAGTCAGAGCCTGATCAGAAGAAGAACGAAGACAAACCACCAAAAGAGGAGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

408

Amino Acids

43.18

Weight (kDa)

4.65

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 2.4e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 3 cut(s) 515, 617, 931
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 5 cut(s) 49, 111, 588, 603, 624
AclWI GGATC 7 cut(s) 653, 665, 666, 678, 965, 1121, 1134
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 2 cut(s) 861, 1116
AfaI GTAC 4 cut(s) 349, 519, 549, 559
AfiI CCNNNNNNNGG 1 cut(s) 579
AgsI TTSAA 4 cut(s) 80, 702, 995, 1155
AjnI CCWGG 4 cut(s) 71, 258, 666, 1096
AjuI GAANNNNNNNTTGG 2 cut(s) 523, 555
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 8 cut(s) 83, 449, 463, 617, 679, 734, 795, 845
AluI AGCT 8 cut(s) 83, 449, 463, 617, 679, 734, 795, 845
Alw21I GWGCWC 2 cut(s) 9, 538
Alw26I GTCTC 2 cut(s) 640, 939
AlwI GGATC 7 cut(s) 653, 665, 666, 678, 965, 1121, 1134
AlwNI CAGNNNCTG 3 cut(s) 617, 620, 1175
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 5 cut(s) 87, 411, 717, 897, 1056
ApoI RAATTY 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 1021
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 4 cut(s) 412, 527, 717, 898
AsuHPI GGTGA 4 cut(s) 148, 260, 446, 562
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BamHI GGATCC 3 cut(s) 658, 670, 1126
BbsI GAAGAC 2 cut(s) 1068, 1200
Bbv12I GWGCWC 2 cut(s) 9, 538
BccI CCATC 3 cut(s) 572, 907, 1088
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 4 cut(s) 73, 260, 668, 1098
BclI TGATCA 1 cut(s) 1177
BcoDI GTCTC 2 cut(s) 640, 939
BfaI CTAG 1 cut(s) 464
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 3 cut(s) 515, 617, 931
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 4 cut(s) 73, 260, 668, 1098
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 4 cut(s) 412, 527, 717, 898
BmiI GGNNCC 3 cut(s) 660, 672, 1128
BmrFI CCNGG 4 cut(s) 73, 260, 668, 1098
BmsI GCATC 6 cut(s) 36, 82, 307, 745, 755, 793
BpiI GAAGAC 2 cut(s) 1068, 1200
BpmI CTGGAG 1 cut(s) 913
Bpu10I CCTNAGC 2 cut(s) 84, 675
BpuEI CTTGAG 1 cut(s) 278
BsaJI CCNNGG 4 cut(s) 159, 750, 960, 1097
BsaWI WCCGGW 2 cut(s) 133, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 579
Bse1I ACTGG 4 cut(s) 198, 825, 1059, 1165
Bse3DI GCAATG 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 4 cut(s) 73, 260, 668, 1098
BseDI CCNNGG 4 cut(s) 159, 750, 960, 1097
BseGI GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BseLI CCNNNNNNNGG 1 cut(s) 579
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 3 cut(s) 98, 666, 837
BseNI ACTGG 4 cut(s) 198, 825, 1059, 1165
BshFI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BsiHKAI GWGCWC 2 cut(s) 9, 538
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 2 cut(s) 134, 573
BslI CCNNNNNNNGG 1 cut(s) 579
BsmAI GTCTC 2 cut(s) 640, 939
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 9, 538
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 5 cut(s) 49, 111, 588, 603, 624
BspANI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BspCNI CTCAG 3 cut(s) 97, 667, 838
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 3 cut(s) 660, 672, 1128
BspMI ACCTGC 3 cut(s) 515, 617, 931
BspPI GGATC 7 cut(s) 653, 665, 666, 678, 965, 1121, 1134
BspQI GCTCTTC 1 cut(s) 996
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 4 cut(s) 198, 825, 1059, 1165
BssECI CCNNGG 4 cut(s) 159, 750, 960, 1097
BssMI GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
BssT1I CCWWGG 2 cut(s) 159, 960
Bst2UI CCWGG 4 cut(s) 73, 260, 668, 1098
Bst4CI ACNGT 3 cut(s) 145, 352, 407
Bst6I CTCTTC 2 cut(s) 705, 996
BstAPI GCANNNNNTGC 3 cut(s) 476, 617, 620
BstC8I GCNNGC 4 cut(s) 223, 227, 681, 1149
BstDEI CTNAG 5 cut(s) 84, 450, 675, 692, 846
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 9 cut(s) 24, 205, 661, 673, 820, 973, 1063, 1129, 1180
BstMAI GTCTC 2 cut(s) 640, 939
BstMBI GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
BstMWI GCNNNNNNNGC 9 cut(s) 287, 476, 608, 611, 614, 617, 620, 623, 743
BstNI CCWGG 4 cut(s) 73, 260, 668, 1098
BstNSI RCATGY 2 cut(s) 225, 229
BstSCI CCNGG 4 cut(s) 71, 258, 666, 1096
BstV2I GAAGAC 2 cut(s) 1068, 1200
BstX2I RGATCY 3 cut(s) 658, 670, 1126
BstYI RGATCY 3 cut(s) 658, 670, 1126
BsuRI GGCC 5 cut(s) 89, 413, 719, 899, 1058
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 2 cut(s) 284, 948
BtsCI GGATG 6 cut(s) 51, 746, 760, 877, 922, 1080
BtsIMutI CAGTG 3 cut(s) 205, 412, 832
BveI ACCTGC 3 cut(s) 515, 617, 931
Cac8I GCNNGC 4 cut(s) 223, 227, 681, 1149
CaiI CAGNNNCTG 3 cut(s) 617, 620, 1175
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 4 cut(s) 412, 527, 717, 898
Csp6I GTAC 4 cut(s) 348, 518, 548, 558
CviAII CATG 7 cut(s) 160, 222, 226, 230, 359, 515, 868
CviQI GTAC 4 cut(s) 348, 518, 548, 558
DdeI CTNAG 5 cut(s) 84, 450, 675, 692, 846
DpnI GATC 9 cut(s) 23, 204, 660, 672, 819, 972, 1062, 1128, 1179
DpnII GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
EaeI YGGCCR 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 705, 996
EarI CTCTTC 2 cut(s) 705, 996
Eco130I CCWWGG 2 cut(s) 159, 960
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 2 cut(s) 861, 1116
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 150
EcoRII CCWGG 4 cut(s) 71, 258, 666, 1096
EcoT14I CCWWGG 2 cut(s) 159, 960
EcoT22I ATGCAT 2 cut(s) 227, 231
ErhI CCWWGG 2 cut(s) 159, 960
FaeI CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 1131, 1163
FatI CATG 7 cut(s) 159, 221, 225, 229, 358, 514, 867
FauI CCCGC 1 cut(s) 581
FbaI TGATCA 1 cut(s) 1177
FokI GGATG 6 cut(s) 58, 733, 767, 884, 929, 1067
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 115
GsuI CTGGAG 1 cut(s) 913
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 5 cut(s) 89, 413, 719, 899, 1058
HapII CCGG 2 cut(s) 134, 573
HhaI GCGC 1 cut(s) 116
Hin1II CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HinfI GANTC 2 cut(s) 214, 1022
HpaII CCGG 2 cut(s) 134, 573
HphI GGTGA 4 cut(s) 148, 260, 446, 562
Hpy166II GTNNAC 3 cut(s) 487, 560, 1105
Hpy188I TCNGA 7 cut(s) 40, 105, 544, 751, 841, 1171, 1182
Hpy188III TCNNGA 5 cut(s) 134, 674, 944, 974, 1007
Hpy8I GTNNAC 3 cut(s) 487, 560, 1105
HpyAV CCTTC 5 cut(s) 381, 578, 1004, 1030, 1140
HpyCH4III ACNGT 3 cut(s) 145, 352, 407
HpyCH4V TGCA 6 cut(s) 27, 225, 229, 806, 905, 1159
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 476, 608, 611, 614, 617, 620, 623, 743
HpyF3I CTNAG 5 cut(s) 84, 450, 675, 692, 846
Hsp92II CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
HspAI GCGC 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 1177
Kzo9I GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 1 cut(s) 70
LweI GCATC 6 cut(s) 36, 82, 307, 745, 755, 793
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 1 cut(s) 187
MalI GATC 9 cut(s) 23, 204, 660, 672, 819, 972, 1062, 1128, 1179
MboI GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 3 cut(s) 658, 670, 1126
MhlI GDGCHC 2 cut(s) 9, 538
MluCI AATT 6 cut(s) 34, 150, 284, 327, 427, 1049
MmeI TCCRAC 2 cut(s) 218, 811
Mph1103I ATGCAT 2 cut(s) 227, 231
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 1021
MseI TTAA 3 cut(s) 99, 395, 1137
MslI CAYNNNNRTG 1 cut(s) 953
MspA1I CMGCKG 2 cut(s) 617, 734
MspI CCGG 2 cut(s) 134, 573
MspR9I CCNGG 4 cut(s) 73, 260, 668, 1098
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 4 cut(s) 73, 260, 668, 1098
MwoI GCNNNNNNNGC 9 cut(s) 287, 476, 608, 611, 614, 617, 620, 623, 743
NcoI CCATGG 1 cut(s) 159
NdeII GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
NlaIII CATG 7 cut(s) 163, 225, 229, 233, 362, 518, 871
NlaIV GGNNCC 3 cut(s) 660, 672, 1128
NmuCI GTSAC 1 cut(s) 187
NsiI ATGCAT 2 cut(s) 227, 231
NspI RCATGY 2 cut(s) 225, 229
PaeI GCATGC 2 cut(s) 225, 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 214, 1022
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 4 cut(s) 71, 258, 666, 1096
PspGI CCWGG 4 cut(s) 71, 258, 666, 1096
PspN4I GGNNCC 3 cut(s) 660, 672, 1128
PspPI GGNCC 4 cut(s) 412, 527, 717, 898
PspXI VCTCGAGB 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 44, 76
PstNI CAGNNNCTG 3 cut(s) 617, 620, 1175
PsuI RGATCY 3 cut(s) 658, 670, 1126
PvuII CAGCTG 2 cut(s) 617, 734
RsaI GTAC 4 cut(s) 349, 519, 549, 559
RsaNI GTAC 4 cut(s) 348, 518, 548, 558
RseI CAYNNNNRTG 1 cut(s) 953
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 3 cut(s) 99, 395, 1137
Sau3AI GATC 9 cut(s) 21, 202, 658, 670, 817, 970, 1060, 1126, 1177
Sau96I GGNCC 4 cut(s) 412, 527, 717, 898
ScaI AGTACT 1 cut(s) 349
ScrFI CCNGG 4 cut(s) 73, 260, 668, 1098
SduI GDGCHC 2 cut(s) 9, 538
SfaNI GCATC 6 cut(s) 36, 82, 307, 745, 755, 793
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 953
SmlI CTYRAG 2 cut(s) 8, 293
SmoI CTYRAG 2 cut(s) 8, 293
SphI GCATGC 2 cut(s) 225, 229
Sse9I AATT 6 cut(s) 34, 150, 284, 327, 427, 1049
SsiI CCGC 5 cut(s) 49, 111, 588, 603, 624
SspMI CTAG 1 cut(s) 464
StyD4I CCNGG 4 cut(s) 71, 258, 666, 1096
StyI CCWWGG 2 cut(s) 159, 960
TaaI ACNGT 3 cut(s) 145, 352, 407
TaqI TCGA 4 cut(s) 9, 911, 943, 1122
TasI AATT 6 cut(s) 34, 150, 284, 327, 427, 1049
TatI WGTACW 3 cut(s) 347, 547, 557
TauI GCSGC 3 cut(s) 114, 605, 626
TfiI GAWTC 2 cut(s) 214, 1022
Tru1I TTAA 3 cut(s) 99, 395, 1137
Tru9I TTAA 3 cut(s) 99, 395, 1137
TscAI CASTG 3 cut(s) 205, 412, 832
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 5 cut(s) 269, 459, 503, 856, 933
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 3 cut(s) 205, 412, 832
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 1 cut(s) 150
XceI RCATGY 2 cut(s) 225, 229
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 1021
XspI CTAG 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 349
Zsp2I ATGCAT 2 cut(s) 227, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.