pycom10g10560

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001533
Physical Location & Seq
Forward (+)
13936906 .. 13940485
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g10560.8

Sequence Viewer

Length: 1200 bp
ATGGTTCTCGAGGCGACTATGATCTGTGTCGACAATTCCGAGTGGATGCGAAACGGCGATTACTCTCCCTCCCGATTGCAAGCTCAAGCCGACGCCATTAATCTCATCTGTGGTGCCAAAACCCAGGCTAATCCGGAGAATACAGTTGGGGTATTGACGATGGCGGGCAAAGGGGTTCGGGTATTGGCTACTCCAACCTCTGATCTTGGAAGGATTTTAGCTTGCATGCATGGTCTTGAGATGGGAGGTGAGATGAACATAGCATCTGCAATCCAGATAGCTCAGCTGGCTCTTAAGCATCGTCAAAACAAAAATCAGCAACAACGGATTATCGTATTTGCTGGAAGTCCTGTCAAATTTGAAAAGAAGGTGTTGGAATCGATAGGAAAGAAACTGAAAAAGAACAGTGTTGCTCTTGACATTGTTGATTTCGGGGAGGAGGATGATGGGAAGCCAGAGAAGCTGGAGGCCCTTCTGTCTGCAGTTAATAATAATGACAGCAGTCATATAGTACATGTCCCCGCTGGTCCAAATGCTCTCTCGGATGTTCTCATAAGTACACCTGTATTTACTGGTGACGGAGAAGGTGGAAGTGGTTTTGCAGTGGCTGCAGCAGCAGCAACTGCTGCTGCTATTGGTGGCTCTGGGTATGACTTTGGCGTTGATCCCAACATTGATCCTGAGCTTGCTCTCGCCCTTCGAGTTTCTATGGAAGAGGAAAGGGCTAGGCAAGAAGCCGCTGCCAAAAGAGCTGCTGAAGAAGCTGGTGGAAAAGGAGGGGAACCATCAACCAAATCAGAAGATGTGACCATGACAGATCAAGTTAACGTTTCTTCCAAGGAGGATAAGAAACATACTGATGATATGGTTGAGGAGAATGACTTGTTGAAGGAGGCTCTTGCAATGTCAATGAACATCTCTGGAACTGGTCATTCAGCAGGTGATACTGAGATGTCAGAAGCGACTAGTGCGGATCAGGAGTTGGCTTTAGCTCTTCAAATGTCAATGCAGGAAAGTGCAGGAGAGCCATCATCCCAGACCGATGTGAGCAAGGTGTTGGAGGATCAATCTTTTGTATCATCTATCCTTGAATCTCTTCCAGGAGTTGACCCCAACGATCCATCAGTGAAAGATCTTCTTGCATCTCTGAAGAATCCGTCTGAGAAGGACAAAGAAGAACCATCGAACGAGGACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

400

Amino Acids

42.08

Weight (kDa)

4.54

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 929
Acc36I ACCTGC 1 cut(s) 929
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 4 cut(s) 164, 522, 738, 971
AclI AACGTT 1 cut(s) 828
AclWI GGATC 5 cut(s) 659, 671, 981, 1071, 1112
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 2 cut(s) 777, 1169
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 513, 559
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 4 cut(s) 362, 889, 998, 1091
AhlI ACTAGT 1 cut(s) 965
AjnI CCWGG 2 cut(s) 123, 1099
AluBI AGCT 9 cut(s) 83, 221, 281, 286, 463, 685, 752, 764, 992
AluI AGCT 9 cut(s) 83, 221, 281, 286, 463, 685, 752, 764, 992
AlwI GGATC 5 cut(s) 659, 671, 981, 1071, 1112
AlwNI CAGNNNCTG 2 cut(s) 608, 623
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 8 cut(s) 608, 611, 614, 617, 626, 629, 740, 752
ApoI RAATTY 1 cut(s) 356
AseI ATTAAT 1 cut(s) 99
Asp700I GAANNNNTTC 1 cut(s) 1095
AspS9I GGNCC 2 cut(s) 469, 527
AsuHPI GGTGA 3 cut(s) 260, 587, 953
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BanI GGYRCC 1 cut(s) 113
BbvI GCAGC 8 cut(s) 595, 613, 616, 623, 626, 629, 727, 739
BccI CCATC 7 cut(s) 154, 235, 440, 793, 1036, 1129, 1189
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 125, 1101
BcuI ACTAGT 1 cut(s) 965
BfaI CTAG 2 cut(s) 726, 966
BfmI CTRYAG 2 cut(s) 480, 609
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 929
BglII AGATCT 1 cut(s) 1132
BisI GCNGC 9 cut(s) 609, 612, 615, 618, 627, 630, 738, 741, 753
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 9 cut(s) 610, 613, 616, 619, 628, 631, 739, 742, 754
Bme1390I CCNGG 2 cut(s) 125, 1101
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 469, 527
BmiI GGNNCC 2 cut(s) 115, 783
BmrFI CCNGG 2 cut(s) 125, 1101
BmsI GCATC 4 cut(s) 36, 272, 307, 1151
BoxI GACNNNNGTC 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 681
Bpu1102I GCTNAGC 1 cut(s) 282
BpuEI CTTGAG 2 cut(s) 69, 257
Bsa29I ATCGAT 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 123, 837
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse1I ACTGG 2 cut(s) 577, 931
Bse3DI GCAATG 1 cut(s) 909
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 2 cut(s) 125, 1101
BseCI ATCGAT 1 cut(s) 380
BseDI CCNNGG 2 cut(s) 123, 837
BseGI GGATG 4 cut(s) 51, 448, 550, 1031
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 4 cut(s) 296, 672, 939, 1152
BseNI ACTGG 2 cut(s) 577, 931
BseRI GAGGAG 2 cut(s) 452, 887
BseXI GCAGC 8 cut(s) 595, 613, 616, 623, 626, 629, 727, 739
BsgI GTGCAG 1 cut(s) 1038
BshFI GGCC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 4 cut(s) 164, 522, 738, 971
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 4 cut(s) 295, 673, 940, 1153
BspDI ATCGAT 1 cut(s) 380
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 115, 783
BspMAI CTGCAG 2 cut(s) 484, 613
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 5 cut(s) 659, 671, 981, 1071, 1112
BspQI GCTCTTC 1 cut(s) 999
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 2 cut(s) 577, 931
BssECI CCNNGG 2 cut(s) 123, 837
BssMI GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 837
Bst2UI CCWGG 2 cut(s) 125, 1101
Bst4CI ACNGT 2 cut(s) 145, 407
Bst6I CTCTTC 3 cut(s) 708, 999, 1101
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 3 cut(s) 608, 623, 626
BstC8I GCNNGC 6 cut(s) 81, 166, 223, 227, 288, 687
BstDEI CTNAG 4 cut(s) 282, 681, 948, 1161
BstF5I GGATG 4 cut(s) 51, 448, 550, 1031
BstKTI GATC 9 cut(s) 24, 205, 667, 679, 820, 976, 1066, 1120, 1135
BstMBI GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
BstNI CCWGG 2 cut(s) 125, 1101
BstNSI RCATGY 2 cut(s) 229, 518
BstPAI GACNNNNGTC 1 cut(s) 501
BstSCI CCNGG 2 cut(s) 123, 1099
BstSFI CTRYAG 2 cut(s) 480, 609
BstV1I GCAGC 8 cut(s) 595, 613, 616, 623, 626, 629, 727, 739
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
Bsu15I ATCGAT 1 cut(s) 380
BsuRI GGCC 1 cut(s) 470
BsuTUI ATCGAT 1 cut(s) 380
BtsCI GGATG 4 cut(s) 51, 448, 550, 1031
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 412, 609, 1131
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 6 cut(s) 81, 166, 223, 227, 288, 687
CaiI CAGNNNCTG 2 cut(s) 608, 623
Cfr13I GGNCC 2 cut(s) 469, 527
ClaI ATCGAT 1 cut(s) 380
CseI GACGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 512, 558
CviAII CATG 4 cut(s) 226, 230, 515, 811
CviQI GTAC 2 cut(s) 512, 558
DdeI CTNAG 4 cut(s) 282, 681, 948, 1161
DpnI GATC 9 cut(s) 23, 204, 666, 678, 819, 975, 1065, 1119, 1134
DpnII GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
Eam1104I CTCTTC 3 cut(s) 708, 999, 1101
EarI CTCTTC 3 cut(s) 708, 999, 1101
Eco130I CCWWGG 1 cut(s) 837
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 2 cut(s) 777, 1169
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 123, 1099
EcoT14I CCWWGG 1 cut(s) 837
EcoT22I ATGCAT 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 837
FaeI CATG 4 cut(s) 229, 233, 518, 814
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 225, 229, 514, 810
FauI CCCGC 2 cut(s) 157, 529
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 9 cut(s) 609, 612, 615, 618, 627, 630, 738, 741, 753
FokI GGATG 4 cut(s) 58, 455, 557, 1018
Fsp4HI GCNGC 9 cut(s) 609, 612, 615, 618, 627, 630, 738, 741, 753
FspBI CTAG 2 cut(s) 726, 966
GluI GCNGC 9 cut(s) 609, 612, 615, 618, 627, 630, 738, 741, 753
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 1 cut(s) 470
HapII CCGG 1 cut(s) 134
HgaI GACGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 4 cut(s) 229, 233, 518, 814
HincII GTYRAC 3 cut(s) 31, 826, 1108
HindII GTYRAC 3 cut(s) 31, 826, 1108
HinfI GANTC 3 cut(s) 377, 1091, 1153
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 260, 587, 953
Hpy166II GTNNAC 4 cut(s) 31, 560, 826, 1108
Hpy188I TCNGA 7 cut(s) 40, 202, 544, 799, 958, 1149, 1162
Hpy188III TCNNGA 9 cut(s) 8, 72, 134, 236, 274, 416, 680, 921, 977
Hpy8I GTNNAC 4 cut(s) 31, 560, 826, 1108
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 7 cut(s) 204, 361, 482, 578, 707, 883, 1159
HpyCH4III ACNGT 2 cut(s) 145, 407
HpyCH4IV ACGT 1 cut(s) 828
HpyF3I CTNAG 4 cut(s) 282, 681, 948, 1161
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 4 cut(s) 229, 233, 518, 814
Kpn2I TCCGGA 1 cut(s) 133
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
LguI GCTCTTC 1 cut(s) 999
Lsp1109I GCAGC 8 cut(s) 595, 613, 616, 623, 626, 629, 727, 739
LweI GCATC 4 cut(s) 36, 272, 307, 1151
MaeI CTAG 2 cut(s) 726, 966
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 2 cut(s) 575, 805
MalI GATC 9 cut(s) 23, 204, 666, 678, 819, 975, 1065, 1119, 1134
MboI GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
MboII GAAGA 9 cut(s) 725, 770, 812, 825, 986, 1088, 1127, 1162, 1187
MflI RGATCY 1 cut(s) 1132
MluCI AATT 2 cut(s) 34, 356
MmeI TCCRAC 3 cut(s) 218, 354, 1038
Mph1103I ATGCAT 1 cut(s) 231
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 4 cut(s) 99, 294, 486, 825
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 3 cut(s) 286, 524, 740
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 125, 1101
MvaI CCWGG 2 cut(s) 125, 1101
NdeII GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
NlaIII CATG 4 cut(s) 229, 233, 518, 814
NlaIV GGNNCC 2 cut(s) 115, 783
NmuCI GTSAC 2 cut(s) 575, 805
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 2 cut(s) 229, 518
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 929
PciI ACATGT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 999
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 3 cut(s) 377, 1091, 1153
PfoI TCCNGGA 1 cut(s) 1099
PkrI GCNGC 9 cut(s) 610, 613, 616, 619, 628, 631, 739, 742, 754
PscI ACATGT 1 cut(s) 514
PshAI GACNNNNGTC 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 123, 1099
PspGI CCWGG 2 cut(s) 123, 1099
PspN4I GGNNCC 2 cut(s) 115, 783
PspPI GGNCC 2 cut(s) 469, 527
PstI CTGCAG 2 cut(s) 484, 613
PstNI CAGNNNCTG 2 cut(s) 608, 623
PsuI RGATCY 1 cut(s) 1132
PvuII CAGCTG 1 cut(s) 286
RsaI GTAC 2 cut(s) 513, 559
RsaNI GTAC 2 cut(s) 512, 558
RseI CAYNNNNRTG 1 cut(s) 858
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 999
SaqAI TTAA 4 cut(s) 99, 294, 486, 825
SatI GCNGC 9 cut(s) 609, 612, 615, 618, 627, 630, 738, 741, 753
Sau3AI GATC 9 cut(s) 21, 202, 664, 676, 817, 973, 1063, 1117, 1132
Sau96I GGNCC 2 cut(s) 469, 527
ScrFI CCNGG 2 cut(s) 125, 1101
SfaNI GCATC 4 cut(s) 36, 272, 307, 1151
SfcI CTRYAG 2 cut(s) 480, 609
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 4 cut(s) 8, 84, 236, 293
SmoI CTYRAG 4 cut(s) 8, 84, 236, 293
SpeI ACTAGT 1 cut(s) 965
SphI GCATGC 1 cut(s) 229
Sse9I AATT 2 cut(s) 34, 356
SsiI CCGC 4 cut(s) 164, 522, 738, 971
SspMI CTAG 2 cut(s) 726, 966
StyD4I CCNGG 2 cut(s) 123, 1099
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 2 cut(s) 145, 407
TaiI ACGT 1 cut(s) 831
TaqI TCGA 5 cut(s) 9, 30, 380, 700, 1184
TaqII GACCGA 1 cut(s) 1055
TasI AATT 2 cut(s) 34, 356
TatI WGTACW 2 cut(s) 511, 557
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 3 cut(s) 377, 1091, 1153
Tru1I TTAA 4 cut(s) 99, 294, 486, 825
Tru9I TTAA 4 cut(s) 99, 294, 486, 825
TscAI CASTG 3 cut(s) 412, 609, 1131
TseFI GTSAC 2 cut(s) 575, 805
TseI GCWGC 8 cut(s) 608, 611, 614, 617, 626, 629, 740, 752
Tsp45I GTSAC 2 cut(s) 575, 805
TspDTI ATGAA 2 cut(s) 269, 926
TspGWI ACGGA 3 cut(s) 340, 594, 1146
TspRI CASTG 3 cut(s) 412, 609, 1131
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 99
XapI RAATTY 1 cut(s) 356
XceI RCATGY 2 cut(s) 229, 518
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 2 cut(s) 726, 966
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.