Rh6BG017300

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001533
Physical Location & Seq
Reverse (-)
2492187 .. 2495773
3587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG017300.1

Sequence Viewer

Length: 1077 bp
ATGGTTCTCGAGGCTACTATGATATGCGTCGACAATTCAGAATGGATGCGAAACGGTGATTACTCCCCTTCTCGGTTCCAAGCTCAAGCCGATGCTATCAATCTCATATGTGGTGCCAAAACCCAGGCCAATCCGGAAAACACAGTTGGGGTGTTGACAATGGCGGGCAAAGGGGTTCGTGTATTGGCCACACCCACTTCTGATCTTGGCAGAATTTTGGCTTGCATGCACGGTCTTGAAATGGGGGGAGAGATGAACATAGCATCCGCAATCCAGGTAGCTCAGTTGGCTCTTAAGCACCGGCAAAACAAAAATCAACAGCAACGGATCATAGTTTTTGCTGGAAGTCCTGTCAAATTTGAAAAGAAGGTATTGGAAATGATAGGGAAGAAATTGAAGAAGAACAGTGTTGCTCTTGATATTGTTGATTTTGGCGAAGAAGATGATGGAAAGCCAGAGAAGCTGGAGGCCCTTCTTTCTGCTGTTAACAATAACGATAGTAGTCATTTAGTACATGTCCCTCCTGGTCCAGATGCTCTCTCTGATGTTCTTATAAGTACACCTGTATTCACTGGTGATGGAGAAGGAGGAAGTGGCTTTGCAGTGGCAGCCGCAGCAGCTACTGCTGCTGCAATTGGTGGTTCTTCCGGCTATGATTTTGACGTAGACCCTAATATAGATCCTGAGCTTGCTCTTGCTCTTAGAGTTTCTATGGAAGAGGAAAGGGCAAGGCAAGAAGCTGCTGCCAAAAGATCGGCTGACGAAGCTAGTAGTAGACAAGGAGAGGAACCGCCATCCAAATCAGAGGATGTAACCATGACAGAAAGTGGTGATGTTTCAGTTGTCAATGAGGATAAGAAACCTACTGATGATAAGGTTGACGAGAATGACTTGCTAAAGCAGGCTCTTGCAATGTCAATGAATATGTCTGGATCCGGTAATTCAGCAGGTGATGCTGAAATGTCAGAGGCAACTAGTGCAGATCAGGAGTTGGCTCTAGGTGTGTTATTGTGTCTCATTGATTTCAGTTATTTAGGGGTGCATGGTAACATTAGTTTTTTTACTAGCGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

358

Amino Acids

37.78

Weight (kDa)

4.56

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 2.6e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 3 cut(s) 30, 666, 775
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 5 cut(s) 164, 267, 612, 791, 1068
AclWI GGATC 4 cut(s) 335, 674, 927, 940
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 213, 356
AfaI GTAC 2 cut(s) 513, 559
AfiI CCNNNNNNNGG 1 cut(s) 72
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 3 cut(s) 239, 362, 397
AhlI ACTAGT 1 cut(s) 974
AjnI CCWGG 3 cut(s) 123, 273, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 7 cut(s) 83, 281, 463, 620, 688, 740, 767
AluI AGCT 7 cut(s) 83, 281, 463, 620, 688, 740, 767
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 4 cut(s) 335, 674, 927, 940
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 3 cut(s) 126, 186, 468
ApeKI GCWGC 7 cut(s) 608, 614, 617, 626, 629, 740, 743
ApoI RAATTY 2 cut(s) 213, 356
AspS9I GGNCC 2 cut(s) 469, 527
AsuHPI GGTGA 4 cut(s) 68, 587, 842, 962
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 527
BalI TGGCCA 1 cut(s) 188
BamHI GGATCC 1 cut(s) 932
BanI GGYRCC 1 cut(s) 113
BbvI GCAGC 7 cut(s) 613, 616, 620, 626, 629, 727, 730
BccI CCATC 3 cut(s) 440, 572, 802
BciT130I CCWGG 3 cut(s) 125, 275, 525
BcoDI GTCTC 1 cut(s) 1019
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 4 cut(s) 768, 975, 998, 1065
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 938
BisI GCNGC 8 cut(s) 609, 612, 615, 618, 627, 630, 741, 744
BlsI GCNGC 8 cut(s) 610, 613, 616, 619, 628, 631, 742, 745
Bme1390I CCNGG 3 cut(s) 125, 275, 525
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 469, 527
BmiI GGNNCC 4 cut(s) 77, 115, 789, 934
BmrFI CCNGG 3 cut(s) 125, 275, 525
BmsI GCATC 5 cut(s) 36, 82, 272, 523, 943
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 133, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 1 cut(s) 918
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 3 cut(s) 125, 275, 525
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 4 cut(s) 51, 263, 794, 814
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 2 cut(s) 296, 675
BseNI ACTGG 1 cut(s) 577
BseXI GCAGC 7 cut(s) 613, 616, 620, 626, 629, 727, 730
BsgI GTGCAG 1 cut(s) 999
BshFI GGCC 3 cut(s) 128, 188, 470
BshNI GGYRCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 134, 301, 648, 936
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 1019
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 3 cut(s) 128, 188, 470
BsoBI CYCGRG 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 6 cut(s) 202, 327, 679, 752, 932, 982
BspACI CCGC 5 cut(s) 164, 267, 612, 791, 1068
BspANI GGCC 3 cut(s) 128, 188, 470
BspCNI CTCAG 2 cut(s) 295, 676
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 77, 115, 789, 934
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 4 cut(s) 335, 674, 927, 940
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 918
BsrFI RCCGGY 1 cut(s) 300
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 6 cut(s) 202, 327, 679, 752, 932, 982
Bst2UI CCWGG 3 cut(s) 125, 275, 525
Bst4CI ACNGT 4 cut(s) 56, 145, 233, 407
Bst6I CTCTTC 1 cut(s) 711
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 3 cut(s) 623, 953, 977
BstC8I GCNNGC 5 cut(s) 166, 223, 227, 690, 903
BstDEI CTNAG 3 cut(s) 282, 684, 701
BstF5I GGATG 4 cut(s) 51, 263, 794, 814
BstKTI GATC 6 cut(s) 205, 330, 682, 755, 935, 985
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 6 cut(s) 202, 327, 679, 752, 932, 982
BstNI CCWGG 3 cut(s) 125, 275, 525
BstNSI RCATGY 2 cut(s) 229, 518
BstSCI CCNGG 3 cut(s) 123, 273, 523
BstV1I GCAGC 7 cut(s) 613, 616, 620, 626, 629, 727, 730
BstX2I RGATCY 2 cut(s) 679, 932
BstYI RGATCY 2 cut(s) 679, 932
BsuRI GGCC 3 cut(s) 128, 188, 470
BtsCI GGATG 4 cut(s) 51, 263, 794, 814
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 412, 570, 609
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 5 cut(s) 166, 223, 227, 690, 903
CaiI CAGNNNCTG 1 cut(s) 623
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 2 cut(s) 469, 527
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 512, 558
CviAII CATG 4 cut(s) 226, 515, 817, 1043
CviQI GTAC 2 cut(s) 512, 558
DdeI CTNAG 3 cut(s) 282, 684, 701
DpnI GATC 6 cut(s) 204, 329, 681, 754, 934, 984
DpnII GATC 6 cut(s) 202, 327, 679, 752, 932, 982
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 3 cut(s) 123, 273, 523
FaeI CATG 4 cut(s) 229, 518, 820, 1046
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 225, 514, 816, 1042
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 107
FblI GTMKAC 3 cut(s) 30, 666, 775
Fnu4HI GCNGC 8 cut(s) 609, 612, 615, 618, 627, 630, 741, 744
FokI GGATG 4 cut(s) 58, 250, 781, 821
Fsp4HI GCNGC 8 cut(s) 609, 612, 615, 618, 627, 630, 741, 744
FspBI CTAG 4 cut(s) 768, 975, 998, 1065
GluI GCNGC 8 cut(s) 609, 612, 615, 618, 627, 630, 741, 744
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 128, 188, 470
HapII CCGG 4 cut(s) 134, 301, 648, 936
HgaI GACGC 1 cut(s) 16
Hin1II CATG 4 cut(s) 229, 518, 820, 1046
HincII GTYRAC 4 cut(s) 31, 156, 487, 880
HindII GTYRAC 4 cut(s) 31, 156, 487, 880
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 4 cut(s) 134, 301, 648, 936
HphI GGTGA 4 cut(s) 68, 587, 842, 962
Hpy166II GTNNAC 7 cut(s) 31, 156, 487, 560, 667, 776, 880
Hpy188I TCNGA 5 cut(s) 40, 202, 544, 805, 967
Hpy188III TCNNGA 8 cut(s) 8, 134, 236, 416, 530, 683, 930, 986
Hpy8I GTNNAC 7 cut(s) 31, 156, 487, 560, 667, 776, 880
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 4 cut(s) 78, 361, 482, 578
HpyCH4III ACNGT 4 cut(s) 56, 145, 233, 407
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 7 cut(s) 225, 229, 602, 632, 911, 980, 1042
HpyF3I CTNAG 3 cut(s) 282, 684, 701
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 4 cut(s) 229, 518, 820, 1046
Kpn2I TCCGGA 1 cut(s) 133
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 6 cut(s) 202, 327, 679, 752, 932, 982
Lsp1109I GCAGC 7 cut(s) 613, 616, 620, 626, 629, 727, 730
LweI GCATC 5 cut(s) 36, 82, 272, 523, 943
MaeI CTAG 4 cut(s) 768, 975, 998, 1065
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 2 cut(s) 811, 1046
MalI GATC 6 cut(s) 204, 329, 681, 754, 934, 984
MboI GATC 6 cut(s) 202, 327, 679, 752, 932, 982
MboII GAAGA 7 cut(s) 400, 409, 412, 449, 452, 636, 728
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 2 cut(s) 679, 932
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 6 cut(s) 34, 213, 356, 392, 633, 940
MluNI TGGCCA 1 cut(s) 188
MnlI CCTC 9 cut(s) 4, 460, 531, 581, 712, 778, 799, 844, 961
Mox20I TGGCCA 1 cut(s) 188
MroI TCCGGA 1 cut(s) 133
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 294, 486
Msp20I TGGCCA 1 cut(s) 188
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 4 cut(s) 134, 301, 648, 936
MspR9I CCNGG 3 cut(s) 125, 275, 525
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 3 cut(s) 125, 275, 525
NdeI CATATG 1 cut(s) 107
NdeII GATC 6 cut(s) 202, 327, 679, 752, 932, 982
NlaIII CATG 4 cut(s) 229, 518, 820, 1046
NlaIV GGNNCC 4 cut(s) 77, 115, 789, 934
NspI RCATGY 2 cut(s) 229, 518
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 938
PciI ACATGT 1 cut(s) 514
PkrI GCNGC 8 cut(s) 610, 613, 616, 619, 628, 631, 742, 745
PscI ACATGT 1 cut(s) 514
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 3 cut(s) 123, 273, 523
PspGI CCWGG 3 cut(s) 123, 273, 523
PspN4I GGNNCC 4 cut(s) 77, 115, 789, 934
PspPI GGNCC 2 cut(s) 469, 527
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 2 cut(s) 679, 932
RsaI GTAC 2 cut(s) 513, 559
RsaNI GTAC 2 cut(s) 512, 558
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 294, 486
SatI GCNGC 8 cut(s) 609, 612, 615, 618, 627, 630, 741, 744
Sau3AI GATC 6 cut(s) 202, 327, 679, 752, 932, 982
Sau96I GGNCC 2 cut(s) 469, 527
ScrFI CCNGG 3 cut(s) 125, 275, 525
SfaNI GCATC 5 cut(s) 36, 82, 272, 523, 943
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 84, 293
SmoI CTYRAG 3 cut(s) 8, 84, 293
SpeI ACTAGT 1 cut(s) 974
SphI GCATGC 1 cut(s) 229
Sse9I AATT 6 cut(s) 34, 213, 356, 392, 633, 940
SsiI CCGC 5 cut(s) 164, 267, 612, 791, 1068
SspMI CTAG 4 cut(s) 768, 975, 998, 1065
StyD4I CCNGG 3 cut(s) 123, 273, 523
TaaI ACNGT 4 cut(s) 56, 145, 233, 407
TaiI ACGT 1 cut(s) 666
TaqI TCGA 2 cut(s) 9, 30
TasI AATT 6 cut(s) 34, 213, 356, 392, 633, 940
TatI WGTACW 2 cut(s) 511, 557
TauI GCSGC 1 cut(s) 614
Tru1I TTAA 2 cut(s) 294, 486
Tru9I TTAA 2 cut(s) 294, 486
TscAI CASTG 3 cut(s) 412, 577, 609
TseI GCWGC 7 cut(s) 608, 614, 617, 626, 629, 740, 743
TspDTI ATGAA 2 cut(s) 269, 935
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 3 cut(s) 412, 577, 609
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 2 cut(s) 213, 356
XceI RCATGY 2 cut(s) 229, 518
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 3 cut(s) 30, 666, 775
XspI CTAG 4 cut(s) 768, 975, 998, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.