RchiOBHm_Chr6g0294421

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001533
Physical Location & Seq
Reverse (-)
56745554 .. 56746840
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26424

Sequence Viewer

Length: 1287 bp
ATGGGAGCATATATAAAAACATGGCCTTCTTGTATTAATCCTCAACAAACACACTTCAGAACCAAAACTTGTCCACCCGCGTACCCAAACTCTGCAACCATGGTTCTCGAGGCGACTATGATATGCATCGACAATTCAGAATGGATGCGAAACAGTGATTACTCTCCCTCCCGATTCCAAGCTCAAGCCGATGCTGTGAATCTCATCTGTGGTATCAAAACCCAGGCCAACCCGGAAAACACAGTTGGGGTGTTGACAATGGCGGGCAAAGGGGTTCGCGTGCTGACCACAACCACCTCTGACCTTGGCAAGATTTTGACTTGCACGCATGGCCTCGAGATAGGGGGTGAGATGAACATAGCAGCTGCAATCCAGGTAGCTCAGTTGGCTCTTAAGCATCGCCAAAATAAAAACCAACAACAAAGGATTGTAGTATTTGCTGGAAGTCCTGTCAAGAATGACAAGAAGGCATTGGAAATAATTGGGAAGAAACTGAAGAAGAACAGTGTTGCTCTTGACATTGTCGATTTTGGAGGAGAAGATGATGGAAAGGCGGAGAAGCTGGAGGCCCTTGTTTCTGCTGTTAATAATAATGGCACTAGTCACATAGTCCATGTACCTCCTGGTCTCCATGCTCTCTCAGATGTTCTTTTAAGTACACCTATATTAACTGGTGATGAAGGAGGAGGAGCTGGCTTTGCAGCCACAGCCGCAGCAGCTGGTATTGGTGGTTCTTCTGGCCATGACTTTGGAATAGATCCCAATATAGATCCCGAGCTTGCTCTCGCCCTTAGAATGTCAATGGAAGAGGAAAGGGCAAGACAAGAAGCTGCTGCTGCCAAAAGAGCTGAGGAAGAAGCTGCTAGCAGACGAGGAAAAGGAGAGGAACGGCCATCCAAATCGGAGGATGTGACCTTGACAGAAAGCGATGATGTTGAAATGGTTGATGAGAATGCCTTGCTAATGCAGGCTCTTGCGATGACAATGAATAACTATGGCTCTAGTAGTTTTGCAGTTGGTGATGCTGAGATGTCAGAGGCAACTGGTGTGGATCAGGACTTGGCTCTAGCTCTTCAAATGTCCATGCAGGAAAGCGCAGGAGGGGCATCATCATCCGAGACGGTTGATGTGAGCAAGGTGTTGGAGGATCAGTCTTTTCTTTCGTCTATCCTGGAATCTCTTCCGGGAGTTGACCCCAATGATCCTTCTGTGAAAGAGTTTCTTGCATCTGTGATTAATCAGTTTGAAAAGAATGAACAAGATCCATCTAATGACGAGGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

428

Amino Acids

45.43

Weight (kDa)

4.63

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 39 - 148 1.4e-31 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 80, 279
AciI CCGC 4 cut(s) 78, 263, 554, 711
AclWI GGATC 6 cut(s) 752, 764, 1059, 1155, 1196, 1256
AcoI YGGCCR 2 cut(s) 739, 890
AcuI CTGAAG 2 cut(s) 40, 515
AfaI GTAC 3 cut(s) 83, 618, 658
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 3 cut(s) 938, 1076, 1247
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 4 cut(s) 222, 372, 622, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
Alw26I GTCTC 2 cut(s) 632, 1112
AlwI GGATC 6 cut(s) 752, 764, 1059, 1155, 1196, 1256
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 3 cut(s) 107, 335, 773
AoxI GGCC 6 cut(s) 23, 225, 331, 567, 739, 890
ApeKI GCWGC 9 cut(s) 362, 365, 701, 713, 716, 830, 833, 836, 860
AseI ATTAAT 2 cut(s) 36, 1236
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 1 cut(s) 1097
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 2 cut(s) 233, 1185
AsuHPI GGTGA 3 cut(s) 359, 686, 1031
AsuNHI GCTAGC 1 cut(s) 863
AvaI CYCGRG 3 cut(s) 107, 335, 773
BaeI ACNNNNGTAYC 2 cut(s) 65, 98
BalI TGGCCA 1 cut(s) 741
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 9 cut(s) 352, 374, 713, 725, 728, 817, 820, 823, 847
BccI CCATC 3 cut(s) 539, 901, 1273
BceAI ACGGC 1 cut(s) 905
BciT130I CCWGG 4 cut(s) 224, 374, 624, 1172
BcnI CCSGG 2 cut(s) 233, 1185
BcoDI GTCTC 2 cut(s) 632, 1112
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 4 cut(s) 600, 864, 1002, 1067
BfrI CTTAAG 1 cut(s) 392
Bme1390I CCNGG 6 cut(s) 224, 233, 374, 624, 1172, 1185
BmeT110I CYCGRG 3 cut(s) 107, 335, 773
BmgT120I GGNCC 1 cut(s) 568
BmrFI CCNGG 6 cut(s) 224, 233, 374, 624, 1172, 1185
BmsI GCATC 7 cut(s) 135, 135, 181, 406, 1012, 1115, 1235
BmtI GCTAGC 1 cut(s) 867
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 849
BpuEI CTTGAG 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 233, 1185
BsaBI GATNNNNATC 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 3 cut(s) 99, 222, 304
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 1136, 1166
Bse1I ACTGG 2 cut(s) 676, 1048
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 4 cut(s) 224, 374, 624, 1172
BseDI CCNNGG 3 cut(s) 99, 222, 304
BseGI GGATG 4 cut(s) 150, 893, 913, 1112
BseJI GATNNNNATC 1 cut(s) 125
BseMII CTCAG 4 cut(s) 395, 654, 840, 1017
BseNI ACTGG 2 cut(s) 676, 1048
BseRI GAGGAG 3 cut(s) 549, 699, 702
BseXI GCAGC 9 cut(s) 352, 374, 713, 725, 728, 817, 820, 823, 847
Bsh1236I CGCG 2 cut(s) 80, 279
BshFI GGCC 6 cut(s) 25, 227, 333, 569, 741, 892
BsiHKCI CYCGRG 3 cut(s) 107, 335, 773
BsiSI CCGG 2 cut(s) 233, 1184
BsmAI GTCTC 2 cut(s) 632, 1112
BsmBI CGTCTC 1 cut(s) 1112
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 6 cut(s) 25, 227, 333, 569, 741, 892
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 3 cut(s) 107, 335, 773
Bsp143I GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 4 cut(s) 78, 263, 554, 711
BspANI GGCC 6 cut(s) 25, 227, 333, 569, 741, 892
BspCNI CTCAG 4 cut(s) 394, 653, 841, 1018
BspFNI CGCG 2 cut(s) 80, 279
BspOI GCTAGC 1 cut(s) 867
BspPI GGATC 6 cut(s) 752, 764, 1059, 1155, 1196, 1256
BspQI GCTCTTC 1 cut(s) 1077
BspTI CTTAAG 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 632
BsrI ACTGG 2 cut(s) 676, 1048
BssECI CCNNGG 3 cut(s) 99, 222, 304
BssMI GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
BssT1I CCWWGG 2 cut(s) 99, 304
Bst2UI CCWGG 4 cut(s) 224, 374, 624, 1172
Bst4CI ACNGT 4 cut(s) 155, 244, 506, 1123
Bst6I CTCTTC 3 cut(s) 801, 1077, 1185
BstAFI CTTAAG 1 cut(s) 392
BstC8I GCNNGC 7 cut(s) 265, 281, 326, 694, 780, 865, 969
BstDEI CTNAG 5 cut(s) 381, 640, 791, 849, 1026
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 4 cut(s) 150, 893, 913, 1112
BstFNI CGCG 2 cut(s) 80, 279
BstHHI GCGC 1 cut(s) 1097
BstKTI GATC 6 cut(s) 760, 772, 1054, 1150, 1204, 1264
BstMAI GTCTC 2 cut(s) 632, 1112
BstMBI GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
BstMWI GCNNNNNNNGC 9 cut(s) 330, 386, 698, 707, 710, 716, 836, 845, 1103
BstNI CCWGG 4 cut(s) 224, 374, 624, 1172
BstSCI CCNGG 6 cut(s) 222, 231, 372, 622, 1170, 1183
BstUI CGCG 2 cut(s) 80, 279
BstV1I GCAGC 9 cut(s) 352, 374, 713, 725, 728, 817, 820, 823, 847
BstX2I RGATCY 3 cut(s) 757, 769, 1261
BstXI CCANNNNNNTGG 1 cut(s) 749
BstYI RGATCY 3 cut(s) 757, 769, 1261
BsuRI GGCC 6 cut(s) 25, 227, 333, 569, 741, 892
BtgI CCRYGG 1 cut(s) 99
BtgZI GCGATG 3 cut(s) 383, 942, 992
BtsCI GGATG 4 cut(s) 150, 893, 913, 1112
BtsIMutI CAGTG 2 cut(s) 160, 511
Cac8I GCNNGC 7 cut(s) 265, 281, 326, 694, 780, 865, 969
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 1 cut(s) 1097
Cfr13I GGNCC 1 cut(s) 568
Csp6I GTAC 3 cut(s) 82, 617, 657
CspCI CAANNNNNGTGG 2 cut(s) 1029, 1064
CviAII CATG 7 cut(s) 21, 100, 329, 614, 632, 743, 1084
CviQI GTAC 3 cut(s) 82, 617, 657
DdeI CTNAG 5 cut(s) 381, 640, 791, 849, 1026
DpnI GATC 6 cut(s) 759, 771, 1053, 1149, 1203, 1263
DpnII GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
EaeI YGGCCR 2 cut(s) 739, 890
Eam1104I CTCTTC 3 cut(s) 801, 1077, 1185
EarI CTCTTC 3 cut(s) 801, 1077, 1185
EciI GGCGGA 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 99, 304
Eco31I GGTCTC 1 cut(s) 632
Eco57I CTGAAG 2 cut(s) 40, 515
Eco88I CYCGRG 3 cut(s) 107, 335, 773
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 4 cut(s) 222, 372, 622, 1170
EcoT14I CCWWGG 2 cut(s) 99, 304
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 99, 304
Esp3I CGTCTC 1 cut(s) 1112
FaeI CATG 7 cut(s) 24, 103, 332, 617, 635, 746, 1087
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FatI CATG 7 cut(s) 20, 99, 328, 613, 631, 742, 1083
FauI CCCGC 2 cut(s) 85, 256
FokI GGATG 4 cut(s) 157, 880, 920, 1099
FspBI CTAG 4 cut(s) 600, 864, 1002, 1067
GlaI GCGC 1 cut(s) 1096
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 6 cut(s) 25, 227, 333, 569, 741, 892
HapII CCGG 2 cut(s) 233, 1184
HhaI GCGC 1 cut(s) 1097
Hin1II CATG 7 cut(s) 24, 103, 332, 617, 635, 746, 1087
Hin6I GCGC 1 cut(s) 1095
HinP1I GCGC 1 cut(s) 1095
HincII GTYRAC 2 cut(s) 255, 1192
HindII GTYRAC 2 cut(s) 255, 1192
HinfI GANTC 3 cut(s) 174, 199, 1175
HpaII CCGG 2 cut(s) 233, 1184
HphI GGTGA 3 cut(s) 359, 686, 1031
Hpy166II GTNNAC 4 cut(s) 74, 255, 659, 1192
Hpy188I TCNGA 7 cut(s) 59, 139, 301, 643, 904, 1036, 1117
Hpy188III TCNNGA 7 cut(s) 107, 171, 337, 454, 515, 773, 1055
Hpy8I GTNNAC 4 cut(s) 74, 255, 659, 1192
HpyAV CCTTC 4 cut(s) 36, 460, 674, 1215
HpyCH4III ACNGT 4 cut(s) 155, 244, 506, 1123
HpyCH4V TGCA 9 cut(s) 95, 126, 324, 368, 701, 967, 1013, 1087, 1226
HpyF10VI GCNNNNNNNGC 9 cut(s) 330, 386, 698, 707, 710, 716, 836, 845, 1103
HpyF3I CTNAG 5 cut(s) 381, 640, 791, 849, 1026
Hsp92II CATG 7 cut(s) 24, 103, 332, 617, 635, 746, 1087
HspAI GCGC 1 cut(s) 1095
Kzo9I GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 2 cut(s) 5, 689
Lsp1109I GCAGC 9 cut(s) 352, 374, 713, 725, 728, 817, 820, 823, 847
LweI GCATC 7 cut(s) 135, 135, 181, 406, 1012, 1115, 1235
MaeI CTAG 4 cut(s) 600, 864, 1002, 1067
MaeIII GTNAC 2 cut(s) 602, 910
MalI GATC 6 cut(s) 759, 771, 1053, 1149, 1203, 1263
MboI GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
MboII GAAGA 9 cut(s) 499, 508, 511, 551, 726, 818, 866, 1064, 1172
MflI RGATCY 3 cut(s) 757, 769, 1261
MlsI TGGCCA 1 cut(s) 741
MluCI AATT 2 cut(s) 133, 480
MluNI TGGCCA 1 cut(s) 741
MmeI TCCRAC 1 cut(s) 1122
Mox20I TGGCCA 1 cut(s) 741
Mph1103I ATGCAT 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 1179
MscI TGGCCA 1 cut(s) 741
MseI TTAA 6 cut(s) 36, 393, 585, 653, 668, 1236
Msp20I TGGCCA 1 cut(s) 741
MspA1I CMGCKG 2 cut(s) 365, 719
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 2 cut(s) 233, 1184
MspR9I CCNGG 6 cut(s) 224, 233, 374, 624, 1172, 1185
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 4 cut(s) 224, 374, 624, 1172
MvnI CGCG 2 cut(s) 80, 279
MwoI GCNNNNNNNGC 9 cut(s) 330, 386, 698, 707, 710, 716, 836, 845, 1103
NciI CCSGG 2 cut(s) 233, 1185
NcoI CCATGG 1 cut(s) 99
NdeII GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
NheI GCTAGC 1 cut(s) 863
NlaIII CATG 7 cut(s) 24, 103, 332, 617, 635, 746, 1087
NmuCI GTSAC 2 cut(s) 602, 910
NsiI ATGCAT 1 cut(s) 128
PaeR7I CTCGAG 2 cut(s) 107, 335
PciSI GCTCTTC 1 cut(s) 1077
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 3 cut(s) 174, 199, 1175
PflFI GACNNNGTC 1 cut(s) 521
PfoI TCCNGGA 2 cut(s) 1170, 1183
PshBI ATTAAT 2 cut(s) 36, 1236
Psp6I CCWGG 4 cut(s) 222, 372, 622, 1170
PspGI CCWGG 4 cut(s) 222, 372, 622, 1170
PspPI GGNCC 1 cut(s) 568
PsrI GAACNNNNNNTAC 2 cut(s) 715, 747
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 3 cut(s) 757, 769, 1261
PsyI GACNNNGTC 1 cut(s) 521
PvuII CAGCTG 2 cut(s) 365, 719
RsaI GTAC 3 cut(s) 83, 618, 658
RsaNI GTAC 3 cut(s) 82, 617, 657
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 6 cut(s) 36, 393, 585, 653, 668, 1236
Sau3AI GATC 6 cut(s) 757, 769, 1051, 1147, 1201, 1261
Sau96I GGNCC 1 cut(s) 568
ScrFI CCNGG 6 cut(s) 224, 233, 374, 624, 1172, 1185
SfaNI GCATC 7 cut(s) 135, 135, 181, 406, 1012, 1115, 1235
Sfr274I CTCGAG 2 cut(s) 107, 335
SlaI CTCGAG 2 cut(s) 107, 335
SmlI CTYRAG 4 cut(s) 107, 183, 335, 392
SmoI CTYRAG 4 cut(s) 107, 183, 335, 392
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 2 cut(s) 133, 480
SsiI CCGC 4 cut(s) 78, 263, 554, 711
SspMI CTAG 4 cut(s) 600, 864, 1002, 1067
StyD4I CCNGG 6 cut(s) 222, 231, 372, 622, 1170, 1183
StyI CCWWGG 2 cut(s) 99, 304
TaaI ACNGT 4 cut(s) 155, 244, 506, 1123
TaqI TCGA 4 cut(s) 108, 129, 336, 525
TasI AATT 2 cut(s) 133, 480
TatI WGTACW 1 cut(s) 656
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 3 cut(s) 174, 199, 1175
Tru1I TTAA 6 cut(s) 36, 393, 585, 653, 668, 1236
Tru9I TTAA 6 cut(s) 36, 393, 585, 653, 668, 1236
TscAI CASTG 2 cut(s) 160, 511
TseFI GTSAC 2 cut(s) 602, 910
TseI GCWGC 9 cut(s) 362, 365, 701, 713, 716, 830, 833, 836, 860
Tsp45I GTSAC 2 cut(s) 602, 910
TspDTI ATGAA 4 cut(s) 368, 693, 1001, 1269
TspRI CASTG 2 cut(s) 160, 511
Tth111I GACNNNGTC 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 392
VspI ATTAAT 2 cut(s) 36, 1236
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XhoI CTCGAG 2 cut(s) 107, 335
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 4 cut(s) 600, 864, 1002, 1067
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.