Prupe.8G163800_v2.0.a1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001533
Physical Location & Seq
Forward (+)
17094165 .. 17098548
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G163800.2

Sequence Viewer

Length: 1221 bp
ATGGTTCTCGAGGCTACTATGATCTGCGTCGACAATTCAGAATGGATGCGGAACGGCGATTACTCTCCGTCCAGATTACAAGCTCAAGCTGACGCTATTAATCTCATTTGTGGTGCCAAAACCCAGGCCAATCCGGAGAATACAGTCGGGGTATTAACAATGGCGGGCAAAGGGGTTCGCGTGTTGGCCACTCCAACCTCTGATCTTGGCAGGATTTTGGCTTGCATGCACGGTCTGGAGATGGGAGGTGAGATGAACATAACAGCTGCGATCCAAGTAGCTCAGTTGGCTCTTAAGCATCGCCAAAACAAAAACCAACAGCAGCGGATTATAGTTTTTGCTGGGAGCCCTGTCAAATATGAGAAGAAGGTGTTGGAAACAATTGGAAAGAAACTGAAAAAGAACAGTGTTGCTCTTGATATTGTTGATTTCGGTGAAGAGGATGATGGGAAGCCAGAGAAGCTGGAGGCCCTTCTGTCTGCTGTTAATAACAATGACAGCAGTCATATAGTACATGTCCCTCCTGGTCCAAATGCTCTCTCTGATGTTCTTATAAGTACACCTGTATTCACTGGTGATGGAGAAGGAGGTAGTGGCTTTGCAGTGGCAGCAGCAGCAGCAACTGCGGCTGCTATTGGTGGTGGTTCTGGCTACGATTTTGGCGTAGATCCCAATATTGATCCTGAGCTCGCTCTTGCCCTTAGAGTTTCTATGGAGGAGGAAAGGGCTAGGCAAGAAGCTGCTGCCAAAAGAGCTGCTGAAGAAGCTGGTAGACAAGAAAAAGGAGAGGAACCATCATCCAAATCAGAGGATGTAACCATGACAGATCAAGCCAATGTTTCTTCTTCCAACGAGGATAAGAAACTTACTGATGATGCGGTTGATGAGAATGACTTGCTGAAGGAGGCTCTTGCAATGTCAATGAATATCTCTGGAACTGGTCATTCAGCAGGTGATACCGAAATTTCAGAGGCAACTAGTGGAGATCAGGAGTTGGCTTTAGCTCTTCAAATGTCCATGCAGGAAAGTGCCGGAGAGCCCTCATCCCAGTCCGATGTCAGCAAGGTGTTGGAGGACCAGTCTTTTCTTTCTTCTATCCTTGAATCACTTCCGGGAGTTGACCCCAATGATCCTTCAGTGAAAGATCTTCTTGCATCTCTGAAGAATCAGTCTGAGTCCCAACAAAAGGATGAAGAAGAACCATCAAACAAGGACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

407

Amino Acids

42.9

Weight (kDa)

4.48

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 941
Acc36I ACCTGC 1 cut(s) 941
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 2 cut(s) 30, 772
AccII CGCG 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 5 cut(s) 49, 164, 325, 626, 878
AclWI GGATC 4 cut(s) 265, 662, 674, 1124
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 963
AcuI CTGAAG 4 cut(s) 780, 920, 1119, 1181
AfaI GTAC 2 cut(s) 513, 559
AfiI CCNNNNNNNGG 1 cut(s) 1186
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 1010, 1103
AhlI ACTAGT 1 cut(s) 977
AjnI CCWGG 2 cut(s) 123, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
Alw21I GWGCWC 1 cut(s) 690
AlwI GGATC 4 cut(s) 265, 662, 674, 1124
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 3 cut(s) 126, 186, 468
ApoI RAATTY 1 cut(s) 963
AseI ATTAAT 1 cut(s) 99
Asp700I GAANNNNTTC 1 cut(s) 1107
AspS9I GGNCC 3 cut(s) 469, 527, 1075
AsuC2I CCSGG 1 cut(s) 1113
AsuHPI GGTGA 4 cut(s) 260, 446, 587, 965
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 527, 1075
BalI TGGCCA 1 cut(s) 188
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 3 cut(s) 350, 690, 1041
Bbv12I GWGCWC 1 cut(s) 690
BccI CCATC 5 cut(s) 235, 440, 572, 802, 1210
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 125, 525
BcnI CCSGG 1 cut(s) 1113
BcuI ACTAGT 1 cut(s) 977
BfaI CTAG 2 cut(s) 729, 978
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 941
BglII AGATCT 1 cut(s) 1144
Bme1390I CCNGG 3 cut(s) 125, 525, 1113
Bme18I GGWCC 2 cut(s) 527, 1075
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 469, 527, 1075
BmiI GGNNCC 3 cut(s) 115, 347, 792
BmrFI CCNGG 3 cut(s) 125, 525, 1113
BmrI ACTGGG 1 cut(s) 1042
BmsI GCATC 4 cut(s) 36, 307, 865, 1163
BmuI ACTGGG 1 cut(s) 1042
BoxI GACNNNNGTC 1 cut(s) 501
BpmI CTGGAG 2 cut(s) 257, 485
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 1113
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 1064, 1094
Bsc4I CCNNNNNNNGG 1 cut(s) 1186
Bse1I ACTGG 4 cut(s) 577, 943, 1048, 1078
Bse3DI GCAATG 1 cut(s) 921
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 2 cut(s) 125, 525
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 6 cut(s) 51, 448, 797, 817, 1043, 1195
BseLI CCNNNNNNNGG 1 cut(s) 1186
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 3 cut(s) 296, 675, 1164
BseNI ACTGG 4 cut(s) 577, 943, 1048, 1078
BseRI GAGGAG 1 cut(s) 731
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 128, 188, 470
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 3 cut(s) 134, 1032, 1112
BslFI GGGAC 2 cut(s) 503, 1162
BslI CCNNNNNNNGG 1 cut(s) 1186
BsmFI GGGAC 2 cut(s) 503, 1162
BsnI GGCC 3 cut(s) 128, 188, 470
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 350, 690, 1041
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
BspACI CCGC 5 cut(s) 49, 164, 325, 626, 878
BspANI GGCC 3 cut(s) 128, 188, 470
BspCNI CTCAG 3 cut(s) 295, 676, 1165
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 115, 347, 792
BspMI ACCTGC 1 cut(s) 941
BspPI GGATC 4 cut(s) 265, 662, 674, 1124
BspQI GCTCTTC 1 cut(s) 1011
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 921
BsrI ACTGG 4 cut(s) 577, 943, 1048, 1078
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
Bst2UI CCWGG 2 cut(s) 125, 525
Bst4CI ACNGT 3 cut(s) 145, 233, 407
Bst6I CTCTTC 2 cut(s) 432, 1011
BstAFI CTTAAG 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 4 cut(s) 166, 223, 227, 690
BstDEI CTNAG 4 cut(s) 282, 684, 701, 1173
BstF5I GGATG 6 cut(s) 51, 448, 797, 817, 1043, 1195
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 9 cut(s) 24, 205, 273, 670, 682, 829, 988, 1132, 1147
BstMBI GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
BstMWI GCNNNNNNNGC 9 cut(s) 287, 460, 608, 614, 617, 623, 626, 752, 764
BstNI CCWGG 2 cut(s) 125, 525
BstNSI RCATGY 2 cut(s) 229, 518
BstPAI GACNNNNGTC 1 cut(s) 501
BstSCI CCNGG 3 cut(s) 123, 523, 1111
BstUI CGCG 1 cut(s) 180
BstX2I RGATCY 2 cut(s) 667, 1144
BstYI RGATCY 2 cut(s) 667, 1144
BsuRI GGCC 3 cut(s) 128, 188, 470
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 6 cut(s) 51, 448, 797, 817, 1043, 1195
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 4 cut(s) 412, 570, 609, 1143
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 4 cut(s) 166, 223, 227, 690
CaiI CAGNNNCTG 1 cut(s) 623
Cfr13I GGNCC 3 cut(s) 469, 527, 1075
CseI GACGC 2 cut(s) 16, 101
Csp6I GTAC 2 cut(s) 512, 558
CviAII CATG 4 cut(s) 226, 515, 820, 1018
CviQI GTAC 2 cut(s) 512, 558
DdeI CTNAG 4 cut(s) 282, 684, 701, 1173
DpnI GATC 9 cut(s) 23, 204, 272, 669, 681, 828, 987, 1131, 1146
DpnII GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 432, 1011
EarI CTCTTC 2 cut(s) 432, 1011
Ecl136II GAGCTC 1 cut(s) 688
Eco24I GRGCYC 3 cut(s) 350, 690, 1041
Eco47I GGWCC 2 cut(s) 527, 1075
Eco53kI GAGCTC 1 cut(s) 688
Eco57I CTGAAG 4 cut(s) 780, 920, 1119, 1181
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 123, 523
EcoT38I GRGCYC 3 cut(s) 350, 690, 1041
FaeI CATG 4 cut(s) 229, 518, 823, 1021
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 2 cut(s) 503, 1162
FatI CATG 4 cut(s) 225, 514, 819, 1017
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 2 cut(s) 30, 772
FokI GGATG 6 cut(s) 58, 455, 784, 824, 1030, 1202
FriOI GRGCYC 3 cut(s) 350, 690, 1041
FspBI CTAG 2 cut(s) 729, 978
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 257, 485
HaeIII GGCC 3 cut(s) 128, 188, 470
HapII CCGG 3 cut(s) 134, 1032, 1112
HgaI GACGC 2 cut(s) 16, 101
Hin1II CATG 4 cut(s) 229, 518, 823, 1021
HincII GTYRAC 2 cut(s) 31, 1120
HindII GTYRAC 2 cut(s) 31, 1120
HinfI GANTC 3 cut(s) 1103, 1165, 1175
HpaII CCGG 3 cut(s) 134, 1032, 1112
HphI GGTGA 4 cut(s) 260, 446, 587, 965
Hpy166II GTNNAC 4 cut(s) 31, 560, 773, 1120
Hpy188I TCNGA 8 cut(s) 40, 202, 544, 808, 970, 1054, 1161, 1174
Hpy188III TCNNGA 8 cut(s) 8, 72, 134, 236, 416, 683, 933, 989
Hpy8I GTNNAC 4 cut(s) 31, 560, 773, 1120
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 5 cut(s) 361, 482, 578, 895, 1143
HpyCH4III ACNGT 3 cut(s) 145, 233, 407
HpyCH4V TGCA 6 cut(s) 225, 229, 602, 914, 1021, 1154
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 460, 608, 614, 617, 623, 626, 752, 764
HpyF3I CTNAG 4 cut(s) 282, 684, 701, 1173
Hsp92II CATG 4 cut(s) 229, 518, 823, 1021
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 1 cut(s) 345
LweI GCATC 4 cut(s) 36, 307, 865, 1163
MaeI CTAG 2 cut(s) 729, 978
MaeIII GTNAC 1 cut(s) 814
MalI GATC 9 cut(s) 23, 204, 272, 669, 681, 828, 987, 1131, 1146
MboI GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 2 cut(s) 667, 1144
MhlI GDGCHC 3 cut(s) 350, 690, 1041
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 3 cut(s) 34, 381, 963
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 1184
MmeI TCCRAC 4 cut(s) 218, 354, 873, 1050
Mox20I TGGCCA 1 cut(s) 188
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 1107
MscI TGGCCA 1 cut(s) 188
MseI TTAA 4 cut(s) 99, 155, 294, 486
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 266, 325
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 3 cut(s) 134, 1032, 1112
MspR9I CCNGG 3 cut(s) 125, 525, 1113
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 2 cut(s) 125, 525
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 287, 460, 608, 614, 617, 623, 626, 752, 764
NciI CCSGG 1 cut(s) 1113
NdeII GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
NlaIII CATG 4 cut(s) 229, 518, 823, 1021
NlaIV GGNNCC 3 cut(s) 115, 347, 792
NspI RCATGY 2 cut(s) 229, 518
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 941
PciI ACATGT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 2 cut(s) 1103, 1165
PfoI TCCNGGA 1 cut(s) 1111
PleI GAGTC 1 cut(s) 1183
PpsI GAGTC 1 cut(s) 1183
PscI ACATGT 1 cut(s) 514
PshAI GACNNNNGTC 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 99
PsiI TTATAA 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 123, 523
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 123, 523
PspN4I GGNNCC 3 cut(s) 115, 347, 792
PspPI GGNCC 3 cut(s) 469, 527, 1075
PsrI GAACNNNNNNTAC 2 cut(s) 44, 76
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 2 cut(s) 667, 1144
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 2 cut(s) 513, 559
RsaNI GTAC 2 cut(s) 512, 558
SacI GAGCTC 1 cut(s) 690
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 4 cut(s) 99, 155, 294, 486
Sau3AI GATC 9 cut(s) 21, 202, 270, 667, 679, 826, 985, 1129, 1144
Sau96I GGNCC 3 cut(s) 469, 527, 1075
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 3 cut(s) 125, 525, 1113
SduI GDGCHC 3 cut(s) 350, 690, 1041
SfaNI GCATC 4 cut(s) 36, 307, 865, 1163
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 2 cut(s) 527, 1075
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 84, 293
SmoI CTYRAG 3 cut(s) 8, 84, 293
SpeI ACTAGT 1 cut(s) 977
SphI GCATGC 1 cut(s) 229
Sse9I AATT 3 cut(s) 34, 381, 963
SsiI CCGC 5 cut(s) 49, 164, 325, 626, 878
SspI AATATT 1 cut(s) 676
SspMI CTAG 2 cut(s) 729, 978
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 3 cut(s) 123, 523, 1111
TaaI ACNGT 3 cut(s) 145, 233, 407
TaqI TCGA 2 cut(s) 9, 30
TasI AATT 3 cut(s) 34, 381, 963
TatI WGTACW 2 cut(s) 511, 557
TauI GCSGC 1 cut(s) 629
TfiI GAWTC 2 cut(s) 1103, 1165
Tru1I TTAA 4 cut(s) 99, 155, 294, 486
Tru9I TTAA 4 cut(s) 99, 155, 294, 486
TscAI CASTG 4 cut(s) 412, 577, 609, 1143
TspDTI ATGAA 3 cut(s) 269, 938, 1206
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 4 cut(s) 412, 577, 609, 1143
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 527, 1075
VspI ATTAAT 1 cut(s) 99
XapI RAATTY 1 cut(s) 963
XceI RCATGY 2 cut(s) 229, 518
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 2 cut(s) 30, 772
XmnI GAANNNNTTC 1 cut(s) 1107
XspI CTAG 2 cut(s) 729, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.