Rroxscaffold_7G00173380

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001533
Physical Location & Seq
Forward (+)
13403836 .. 13405029
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173380.1

Sequence Viewer

Length: 1194 bp
ATGGTTCTCGAGGCGACTATGATATGCATCGACAATTCAGAATGGATGCGAAACAGTGATTACTCTCCCACCCGATTCCAAGCTCAAGCCGATGCTGTGAATCTCATCTGTGGTATCAAAACCCAGGCCAACCCGGAAAACACAGTTGGGGTGTTGACAATGGCGGGCAAAGGGGTTCGCGTGCTGACCACAACCACCTCTGACCTTGGCAAGATTTTGACTTGCACGCATGGCCTCGAGATAGGGGGTGAGATGAACATAGCAGCTGCAATCCAGGTAGCTCAGTTGGCTCTTAAGCATCGCCAAAATAAAAACCAACAACAAAGGATTGTAGTATTTGCTGGAAGTCCTGTCAAGAATGACAAGAAGGCATTGGAAATAATTGGGAAGAAACTGAAGAAGAACAGTGTTGCTCTTGACATTGTCGTTTTTGGAGGAGAAGATGATGGAAAGGCGGAGAAGCTGGAGGCCCTTGTTTCTGCTGTTAATAATAATGGCACTAGTCACATAGTCCATGTACCTCCTGGTCTCCATGCTCTCTCTGATGTTCTTTTAAGTACACCCATATTAACTGGTGATGAAGAAGGAGGAGCTGGCTTTGCAGCCACAGCCGCAGCAGCTGGTATTGGTGGTTCTTCTGGCCATGACTTTGGAGTAGATCCCAATATAGATCCCGAGCTTGCTCTCGCCCTTAGAATGTCAATGGAAGAGGAAAGGGCAAGACAAGAAGCTGCTGCTGCCAAAAGAGCTGAGGAAGAAGCTGCTAGCAGACGAGGAAAAGGAGAGGAACGGCCATCCAAATCGGAGGATGTGACCATGACAGAAAGCGATGATGTTGAAATGGTTGATGAGAATGCCTTGCTAATGCAGGCTATTGCGATGTCAATGAATAACTATGGCTCTGGTAGTTCTCAAGTTGGTGATGCTGAGATGTCAGAGGCAACTGGTGTACGTGTGGATCAGGACTTGGCTCTAGCTCTTCAAATGTCCATGCAGGAAAGCGCAGGAGGGGCATCATCATCCGAGACAGTTGATGTGAGCAAGGTGTTGGAGGATCAGTCTTTTCTTTCGTCTATCCTGGAATCTCTTCCGGGAGTTGACCCCGATGATCCTTCTGTGAAAGAGTTTCTTGCATCTGTGATTAATCAGTTTCAAAAGAATGAACAAGATCCATCTAATGACGAGGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

397

Amino Acids

42.02

Weight (kDa)

4.54

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 6 - 115 6.4e-32 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 180
AciI CCGC 3 cut(s) 164, 455, 612
AclWI GGATC 6 cut(s) 653, 665, 966, 1062, 1103, 1163
AcoI YGGCCR 2 cut(s) 640, 791
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 3 cut(s) 519, 559, 951
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 952
AgsI TTSAA 3 cut(s) 839, 983, 1154
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 4 cut(s) 123, 273, 523, 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
Alw26I GTCTC 2 cut(s) 533, 1019
AlwI GGATC 6 cut(s) 653, 665, 966, 1062, 1103, 1163
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 3 cut(s) 8, 236, 674
AoxI GGCC 5 cut(s) 126, 232, 468, 640, 791
ApeKI GCWGC 9 cut(s) 263, 266, 602, 614, 617, 731, 734, 737, 761
AseI ATTAAT 1 cut(s) 1143
Asp700I GAANNNNTTC 1 cut(s) 1086
AspLEI GCGC 1 cut(s) 1004
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 2 cut(s) 134, 1092
AsuHPI GGTGA 3 cut(s) 260, 587, 932
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 3 cut(s) 8, 236, 674
BalI TGGCCA 1 cut(s) 642
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
BccI CCATC 3 cut(s) 440, 802, 1180
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 4 cut(s) 125, 275, 525, 1079
BcnI CCSGG 2 cut(s) 134, 1092
BcoDI GTCTC 2 cut(s) 533, 1019
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 3 cut(s) 501, 765, 974
BfrI CTTAAG 1 cut(s) 293
Bme1390I CCNGG 6 cut(s) 125, 134, 275, 525, 1079, 1092
BmeT110I CYCGRG 3 cut(s) 8, 236, 674
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 6 cut(s) 125, 134, 275, 525, 1079, 1092
BmsI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1022, 1142
BmtI GCTAGC 1 cut(s) 768
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 750
BpuEI CTTGAG 2 cut(s) 69, 897
BpuMI CCSGG 2 cut(s) 134, 1092
BsaAI YACGTR 1 cut(s) 953
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 2 cut(s) 123, 205
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bse1I ACTGG 2 cut(s) 577, 949
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 4 cut(s) 125, 275, 525, 1079
BseDI CCNNGG 2 cut(s) 123, 205
BseGI GGATG 4 cut(s) 51, 794, 814, 1019
BseJI GATNNNNATC 1 cut(s) 26
BseMII CTCAG 3 cut(s) 296, 741, 918
BseNI ACTGG 2 cut(s) 577, 949
BseRI GAGGAG 2 cut(s) 450, 603
BseXI GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 128, 234, 470, 642, 793
BsiHKCI CYCGRG 3 cut(s) 8, 236, 674
BsiSI CCGG 2 cut(s) 134, 1091
BsmAI GTCTC 2 cut(s) 533, 1019
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 5 cut(s) 128, 234, 470, 642, 793
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 3 cut(s) 8, 236, 674
Bsp143I GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
BspACI CCGC 3 cut(s) 164, 455, 612
BspANI GGCC 5 cut(s) 128, 234, 470, 642, 793
BspCNI CTCAG 3 cut(s) 295, 742, 919
BspFNI CGCG 1 cut(s) 180
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 6 cut(s) 653, 665, 966, 1062, 1103, 1163
BspQI GCTCTTC 1 cut(s) 984
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 533
BsrI ACTGG 2 cut(s) 577, 949
BssECI CCNNGG 2 cut(s) 123, 205
BssMI GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 4 cut(s) 125, 275, 525, 1079
Bst4CI ACNGT 4 cut(s) 56, 145, 407, 1030
Bst6I CTCTTC 3 cut(s) 702, 984, 1092
BstAFI CTTAAG 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 953
BstC8I GCNNGC 7 cut(s) 166, 182, 227, 595, 681, 766, 870
BstDEI CTNAG 4 cut(s) 282, 692, 750, 927
BstF5I GGATG 4 cut(s) 51, 794, 814, 1019
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 1004
BstKTI GATC 6 cut(s) 661, 673, 961, 1057, 1111, 1171
BstMAI GTCTC 2 cut(s) 533, 1019
BstMBI GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
BstMWI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1010
BstNI CCWGG 4 cut(s) 125, 275, 525, 1079
BstSCI CCNGG 6 cut(s) 123, 132, 273, 523, 1077, 1090
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
BstX2I RGATCY 3 cut(s) 658, 670, 1168
BstXI CCANNNNNNTGG 1 cut(s) 650
BstYI RGATCY 3 cut(s) 658, 670, 1168
BsuRI GGCC 5 cut(s) 128, 234, 470, 642, 793
BtgZI GCGATG 3 cut(s) 284, 843, 893
BtsCI GGATG 4 cut(s) 51, 794, 814, 1019
BtsIMutI CAGTG 2 cut(s) 61, 412
Cac8I GCNNGC 7 cut(s) 166, 182, 227, 595, 681, 766, 870
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 1004
Cfr13I GGNCC 1 cut(s) 469
Csp6I GTAC 3 cut(s) 518, 558, 950
CviAII CATG 6 cut(s) 230, 515, 533, 644, 817, 991
CviQI GTAC 3 cut(s) 518, 558, 950
DdeI CTNAG 4 cut(s) 282, 692, 750, 927
DpnI GATC 6 cut(s) 660, 672, 960, 1056, 1110, 1170
DpnII GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
EaeI YGGCCR 2 cut(s) 640, 791
Eam1104I CTCTTC 3 cut(s) 702, 984, 1092
EarI CTCTTC 3 cut(s) 702, 984, 1092
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 3 cut(s) 8, 236, 674
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 4 cut(s) 123, 273, 523, 1077
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 6 cut(s) 233, 518, 536, 647, 820, 994
FalI AAGNNNNNCTT 2 cut(s) 1113, 1145
FatI CATG 6 cut(s) 229, 514, 532, 643, 816, 990
FauI CCCGC 1 cut(s) 157
FokI GGATG 4 cut(s) 58, 781, 821, 1006
FspBI CTAG 3 cut(s) 501, 765, 974
GlaI GCGC 1 cut(s) 1003
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 5 cut(s) 128, 234, 470, 642, 793
HapII CCGG 2 cut(s) 134, 1091
HhaI GCGC 1 cut(s) 1004
Hin1II CATG 6 cut(s) 233, 518, 536, 647, 820, 994
Hin6I GCGC 1 cut(s) 1002
HinP1I GCGC 1 cut(s) 1002
HincII GTYRAC 2 cut(s) 156, 1099
HindII GTYRAC 2 cut(s) 156, 1099
HinfI GANTC 3 cut(s) 75, 100, 1082
HpaII CCGG 2 cut(s) 134, 1091
HphI GGTGA 3 cut(s) 260, 587, 932
Hpy166II GTNNAC 4 cut(s) 156, 560, 950, 1099
Hpy188I TCNGA 6 cut(s) 40, 202, 544, 805, 937, 1024
Hpy188III TCNNGA 6 cut(s) 8, 238, 355, 416, 674, 962
Hpy8I GTNNAC 4 cut(s) 156, 560, 950, 1099
HpyAV CCTTC 3 cut(s) 361, 578, 1122
HpyCH4III ACNGT 4 cut(s) 56, 145, 407, 1030
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 7 cut(s) 27, 225, 269, 602, 868, 994, 1133
HpyF10VI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1010
HpyF3I CTNAG 4 cut(s) 282, 692, 750, 927
HpySE526I ACGT 1 cut(s) 952
Hsp92II CATG 6 cut(s) 233, 518, 536, 647, 820, 994
HspAI GCGC 1 cut(s) 1002
Kzo9I GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 9 cut(s) 253, 275, 614, 626, 629, 718, 721, 724, 748
LweI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1022, 1142
MaeI CTAG 3 cut(s) 501, 765, 974
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 2 cut(s) 503, 811
MalI GATC 6 cut(s) 660, 672, 960, 1056, 1110, 1170
MboI GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
MflI RGATCY 3 cut(s) 658, 670, 1168
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 2 cut(s) 34, 381
MluNI TGGCCA 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 1029
Mox20I TGGCCA 1 cut(s) 642
Mph1103I ATGCAT 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1086
MscI TGGCCA 1 cut(s) 642
MseI TTAA 5 cut(s) 294, 486, 554, 569, 1143
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 2 cut(s) 266, 620
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 2 cut(s) 134, 1091
MspR9I CCNGG 6 cut(s) 125, 134, 275, 525, 1079, 1092
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 4 cut(s) 125, 275, 525, 1079
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 231, 287, 599, 608, 611, 617, 737, 746, 1010
NciI CCSGG 2 cut(s) 134, 1092
NdeII GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 6 cut(s) 233, 518, 536, 647, 820, 994
NmuCI GTSAC 2 cut(s) 503, 811
NsiI ATGCAT 1 cut(s) 29
PaeR7I CTCGAG 2 cut(s) 8, 236
PciSI GCTCTTC 1 cut(s) 984
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 3 cut(s) 75, 100, 1082
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 1077, 1090
Ppu21I YACGTR 1 cut(s) 953
PshBI ATTAAT 1 cut(s) 1143
Psp6I CCWGG 4 cut(s) 123, 273, 523, 1077
PspGI CCWGG 4 cut(s) 123, 273, 523, 1077
PspPI GGNCC 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 658, 670, 1168
PsyI GACNNNGTC 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 266, 620
RsaI GTAC 3 cut(s) 519, 559, 951
RsaNI GTAC 3 cut(s) 518, 558, 950
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 5 cut(s) 294, 486, 554, 569, 1143
Sau3AI GATC 6 cut(s) 658, 670, 958, 1054, 1108, 1168
Sau96I GGNCC 1 cut(s) 469
ScrFI CCNGG 6 cut(s) 125, 134, 275, 525, 1079, 1092
SfaNI GCATC 7 cut(s) 36, 36, 82, 307, 913, 1022, 1142
Sfr274I CTCGAG 2 cut(s) 8, 236
SlaI CTCGAG 2 cut(s) 8, 236
SmlI CTYRAG 5 cut(s) 8, 84, 236, 293, 912
SmoI CTYRAG 5 cut(s) 8, 84, 236, 293, 912
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 2 cut(s) 34, 381
SsiI CCGC 3 cut(s) 164, 455, 612
SspMI CTAG 3 cut(s) 501, 765, 974
StyD4I CCNGG 6 cut(s) 123, 132, 273, 523, 1077, 1090
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 4 cut(s) 56, 145, 407, 1030
TaiI ACGT 1 cut(s) 955
TaqI TCGA 3 cut(s) 9, 30, 237
TasI AATT 2 cut(s) 34, 381
TatI WGTACW 1 cut(s) 557
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 3 cut(s) 75, 100, 1082
Tru1I TTAA 5 cut(s) 294, 486, 554, 569, 1143
Tru9I TTAA 5 cut(s) 294, 486, 554, 569, 1143
TscAI CASTG 2 cut(s) 61, 412
TseFI GTSAC 2 cut(s) 503, 811
TseI GCWGC 9 cut(s) 263, 266, 602, 614, 617, 731, 734, 737, 761
Tsp45I GTSAC 2 cut(s) 503, 811
TspDTI ATGAA 4 cut(s) 269, 594, 902, 1176
TspRI CASTG 2 cut(s) 61, 412
Tth111I GACNNNGTC 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 2 cut(s) 8, 236
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 3 cut(s) 501, 765, 974
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.