AT5G12320

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006700
Physical Location & Seq
Forward (+)
3982577 .. 3984112
1536 bp
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UTR
Exon/CDS
Intron
AT5G12320.1

Sequence Viewer

Length: 435 bp
ATGGGAGCAGAGACAAACGGAGTTGCAGAGAAAGTCGACGACTTGCTGGAGGCTGCTAGATACAATGATATTGATGATCTCAGAACCTTGGCCTCTGATGGTCTCTCTCTTCACTCTCGTGATTCACAAGGCCGAACAGCCCTGCATATGGCCGCAGCGAATGGACATATGACTATAGTGGAGTATCTTATCAGTGAAGGAGTGGATATCAACGCTCTTAACGATGAGAACAATGCTCCTCTACATTGGGCATGCTTGAATGGCCATGTCGAGGTGGTAAAGAGATTGATCTTGGCTGGAGCAAGTCTAAGTCTTTTGAATCGGTACGAGAGAACTCCAATGGATGAAGCTATTGGTGCGGAGAAGATGGAGATTATAGATGCTATTAACACAACTGTTGCCCAAATGGAACTTGAAAATACCAGTATGACATGA

Protein Analysis

144

Amino Acids

15.66

Weight (kDa)

4.5

Isoelectric Point (pI)

16.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 103 4.6e-19 Ankyrin repeats (3 copies)
Ank_4 PF13637 16 - 64 6.6e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 33 - 82 2.9e-09 Ankyrin repeats (many copies)
Ank PF00023 43 - 75 1.3e-09 Ankyrin repeat
Ank_3 PF13606 44 - 72 1.5e-07 Ankyrin repeat
Ank_4 PF13637 55 - 97 5.6e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 67 - 117 1.2e-07 Ankyrin repeats (many copies)
Ank PF00023 78 - 107 1.1e-06 Ankyrin repeat
Ank_4 PF13637 81 - 126 4.1e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 36
AciI CCGC 2 cut(s) 153, 359
AcoI YGGCCR 2 cut(s) 150, 262
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 2 cut(s) 148, 271
AgsI TTSAA 3 cut(s) 259, 319, 416
AleI CACNNNNGTG 1 cut(s) 117
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 5, 107
AoxI GGCC 4 cut(s) 90, 130, 150, 262
ApeKI GCWGC 2 cut(s) 53, 155
BalI TGGCCA 1 cut(s) 264
BauI CACGAG 1 cut(s) 117
BbvI GCAGC 2 cut(s) 40, 167
BccI CCATC 2 cut(s) 92, 361
BcoDI GTCTC 2 cut(s) 5, 107
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 3 cut(s) 54, 153, 156
BlsI GCNGC 3 cut(s) 55, 154, 157
BmsI GCATC 1 cut(s) 370
BpmI CTGGAG 2 cut(s) 68, 318
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 271
Bse1I ACTGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 2 cut(s) 148, 271
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 40, 167
BshFI GGCC 4 cut(s) 92, 132, 152, 264
BslI CCNNNNNNNGG 2 cut(s) 148, 271
BsmAI GTCTC 2 cut(s) 5, 107
BsnI GGCC 4 cut(s) 92, 132, 152, 264
Bso31I GGTCTC 1 cut(s) 107
Bsp143I GATC 2 cut(s) 76, 288
BspACI CCGC 2 cut(s) 153, 359
BspANI GGCC 4 cut(s) 92, 132, 152, 264
BspCNI CTCAG 1 cut(s) 93
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 2 cut(s) 76, 288
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 2 cut(s) 80, 308
BstF5I GGATG 1 cut(s) 349
BstKTI GATC 2 cut(s) 79, 291
BstMAI GTCTC 2 cut(s) 5, 107
BstMBI GATC 2 cut(s) 76, 288
BstMWI GCNNNNNNNGC 2 cut(s) 261, 356
BstNSI RCATGY 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 40, 167
BsuRI GGCC 4 cut(s) 92, 132, 152, 264
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 325
CviAII CATG 3 cut(s) 252, 266, 432
CviJI RGCY 8 cut(s) 53, 92, 132, 140, 152, 264, 296, 350
CviKI_1 RGCY 8 cut(s) 53, 92, 132, 140, 152, 264, 296, 350
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 2 cut(s) 80, 308
DpnI GATC 2 cut(s) 78, 290
DpnII GATC 2 cut(s) 76, 288
EaeI YGGCCR 2 cut(s) 150, 262
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 208
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 3 cut(s) 255, 269, 435
FatI CATG 3 cut(s) 251, 265, 431
FauNDI CATATG 2 cut(s) 147, 168
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 3 cut(s) 54, 153, 156
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 54, 153, 156
FspBI CTAG 1 cut(s) 57
GluI GCNGC 3 cut(s) 54, 153, 156
GsuI CTGGAG 2 cut(s) 68, 318
HaeIII GGCC 4 cut(s) 92, 132, 152, 264
Hin1II CATG 3 cut(s) 255, 269, 435
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 2 cut(s) 122, 319
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 2 cut(s) 83, 97
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4V TGCA 2 cut(s) 26, 145
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 356
HpyF3I CTNAG 2 cut(s) 80, 308
Hsp92II CATG 3 cut(s) 255, 269, 435
Kzo9I GATC 2 cut(s) 76, 288
LmnI GCTCC 3 cut(s) 5, 241, 299
LpnPI CCDG 3 cut(s) 32, 155, 282
Lsp1109I GCAGC 2 cut(s) 40, 167
LweI GCATC 1 cut(s) 370
MaeI CTAG 1 cut(s) 57
MalI GATC 2 cut(s) 78, 290
MboI GATC 2 cut(s) 76, 288
MboII GAAGA 2 cut(s) 101, 376
MlsI TGGCCA 1 cut(s) 264
MluNI TGGCCA 1 cut(s) 264
MnlI CCTC 4 cut(s) 43, 103, 249, 265
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 219, 387
MslI CAYNNNNRTG 1 cut(s) 117
Msp20I TGGCCA 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 261, 356
NdeI CATATG 2 cut(s) 147, 168
NdeII GATC 2 cut(s) 76, 288
NlaIII CATG 3 cut(s) 255, 269, 435
NspI RCATGY 1 cut(s) 255
OliI CACNNNNGTG 1 cut(s) 117
PaeI GCATGC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 2 cut(s) 122, 319
PkrI GCNGC 3 cut(s) 55, 154, 157
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 117
SalI GTCGAC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 219, 387
SatI GCNGC 3 cut(s) 54, 153, 156
Sau3AI GATC 2 cut(s) 76, 288
SetI ASST 3 cut(s) 89, 276, 352
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 117
SphI GCATGC 1 cut(s) 255
SsiI CCGC 2 cut(s) 153, 359
SspMI CTAG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 36, 270
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 2 cut(s) 122, 319
Tru1I TTAA 2 cut(s) 219, 387
Tru9I TTAA 2 cut(s) 219, 387
TscAI CASTG 1 cut(s) 199
TseI GCWGC 2 cut(s) 53, 155
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 199
XceI RCATGY 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 36
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.