Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006700

Physical Location & Seq

Genomic Coordinates Reverse (-)

22354246 .. 22358210

Gene Structure 3965 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1167000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 462 bp

ATGGGGGAAGGGAGAGATAAAGTTGGTGATTCTCCAGTCGAAACGGCATCATTGGAGAGGGTAGAGGCCTTGCTGGAGGCTGCTAGGTACGATGACATTGATGATCTTAAAAGCTTAGAATCTGCTCGTCTTTCTCTTGATTCTAAGGATCTCCACGGCCGAACAGCACTGCACATGGCTTCTGCTAATGGGCATCTTCAGATTGTGGAGTATCTAATCAGCAGAGGAGTGGATCTGAATGCTACAAATGAGGAGATGAATACACCTCTACACTGGGCTTGCCTGAATGGACATGTTGAGGTGATTAAGAAATTGATCTTAGCAGGAGCAAATCTTAGTGTATTAAACAGCTACGAAAGGACTCCAGTTGATGAAGCAGTGAGCAGGGGAAAGATGGATGTTCTGGATGCCGTTAATGTAGCGGCTGCCCAAGTAGAGCTTCGTGACGTCAGCGTGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000054 GO:0000056 GO:0005575 GO:0006403 GO:0006405 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0015931 GO:0022613 GO:0030684 GO:0030688 GO:0031503 GO:0032991 GO:0033036 GO:0033750 GO:0034613 GO:0042254 GO:0042274 GO:0042886 GO:0044085 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071705 GO:0071840 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ank_5

Protein Analysis

154

Amino Acids

16.59

Weight (kDa)

4.77

Isoelectric Point (pI)

21.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	24 - 113	2.6e-17	Ankyrin repeats (3 copies)
Ank_4	PF13637	27 - 73	6.9e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	45 - 91	1.6e-11	Ankyrin repeats (many copies)
Ank	PF00023	52 - 83	5.8e-10	Ankyrin repeat
Ank_3	PF13606	52 - 81	2e-07	Ankyrin repeat
Ank_4	PF13637	64 - 106	3.3e-12	Ankyrin repeats (many copies)
Ank_5	PF13857	72 - 126	3.8e-09	Ankyrin repeats (many copies)
Ank_2	PF12796	77 - 136	4e-08	Ankyrin repeats (3 copies)
Ank	PF00023	87 - 116	8.7e-08	Ankyrin repeat
Ank_3	PF13606	87 - 113	1.8e-06	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006700)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12320
fragaria_vesca	FvH4_1g25870
malus_domestica	MD03G1167000.v1.1 MD11G1186200.v1.1
prunus_persica	Prupe.4G267400_v2.0.a1 Prupe.4G267400_v2.0.a1
pyrus_communis	pycom03g12240 pycom12g08670
rosa_chinensis	RchiOBHm_Chr3g0485011
rosa_laevigata	RLG00000000958 RLG00000004598 RLG00000008951 RLG00000023176 RLG00000023289 RLG00000031269
rosa_multiflora	Rmu_sc0006040.1_g000021 Rmu_sc0007628.1_g000001 Rmu_sc0008046.1_g000002
rosa_roxburghii	Rroxscaffold_6G00397260 Rroxscaffold_6G00413790
rosa_rugosa	Rorug03G0215100
rosa_samantha	Rh2BG228600 Rh3AG265500 Rh3BG301100 Rh3CG299400 Rh3DG295200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	450
AciI	CCGC	1 cut(s)	422
AclWI	GGATC	2 cut(s)	156, 240
AcoI	YGGCCR	1 cut(s)	157
AcuI	CTGAAG	1 cut(s)	182
AcyI	GRCGYC	1 cut(s)	447
AfaI	GTAC	1 cut(s)	89
AflIII	ACRYGT	1 cut(s)	292
AjuI	GAANNNNNNNTTGG	2 cut(s)	423, 455
AluBI	AGCT	3 cut(s)	114, 351, 439
AluI	AGCT	3 cut(s)	114, 351, 439
AlwI	GGATC	2 cut(s)	156, 240
AoxI	GGCC	2 cut(s)	66, 157
ApeKI	GCWGC	2 cut(s)	80, 425
AsuHPI	GGTGA	2 cut(s)	38, 313
BbvI	GCAGC	2 cut(s)	67, 412
BccI	CCATC	1 cut(s)	388
BceAI	ACGGC	3 cut(s)	60, 172, 395
BfaI	CTAG	2 cut(s)	84, 460
BisI	GCNGC	3 cut(s)	81, 423, 426
BlsI	GCNGC	3 cut(s)	82, 424, 427
BmrI	ACTGGG	1 cut(s)	283
BmsI	GCATC	3 cut(s)	56, 202, 397
BmuI	ACTGGG	1 cut(s)	283
BpmI	CTGGAG	3 cut(s)	18, 95, 348
BsaHI	GRCGYC	1 cut(s)	447
BsaJI	CCNNGG	1 cut(s)	154
Bse1I	ACTGG	3 cut(s)	35, 278, 365
BseDI	CCNNGG	1 cut(s)	154
BseGI	GGATG	2 cut(s)	403, 412
BseNI	ACTGG	3 cut(s)	35, 278, 365
BseRI	GAGGAG	2 cut(s)	240, 266
BseX3I	CGGCCG	1 cut(s)	157
BseXI	GCAGC	2 cut(s)	67, 412
BsgI	GTGCAG	1 cut(s)	155
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	2 cut(s)	68, 159
BsiEI	CGRYCG	1 cut(s)	160
BsmI	GAATGC	1 cut(s)	244
BsnI	GGCC	2 cut(s)	68, 159
Bsp143I	GATC	4 cut(s)	103, 148, 232, 315
BspACI	CCGC	1 cut(s)	422
BspANI	GGCC	2 cut(s)	68, 159
BspPI	GGATC	2 cut(s)	156, 240
BsrI	ACTGG	3 cut(s)	35, 278, 365
BssECI	CCNNGG	1 cut(s)	154
BssMI	GATC	4 cut(s)	103, 148, 232, 315
BssNI	GRCGYC	1 cut(s)	447
BstACI	GRCGYC	1 cut(s)	447
BstC8I	GCNNGC	1 cut(s)	280
BstDEI	CTNAG	4 cut(s)	115, 144, 319, 335
BstDSI	CCRYGG	1 cut(s)	154
BstF5I	GGATG	2 cut(s)	403, 412
BstKTI	GATC	4 cut(s)	106, 151, 235, 318
BstMBI	GATC	4 cut(s)	103, 148, 232, 315
BstMCI	CGRYCG	1 cut(s)	160
BstNSI	RCATGY	1 cut(s)	296
BstV1I	GCAGC	2 cut(s)	67, 412
BstX2I	RGATCY	2 cut(s)	148, 232
BstYI	RGATCY	2 cut(s)	148, 232
BstZI	CGGCCG	1 cut(s)	157
BsuRI	GGCC	2 cut(s)	68, 159
BtgI	CCRYGG	1 cut(s)	154
BtsCI	GGATG	2 cut(s)	403, 412
BtsI	GCAGTG	2 cut(s)	167, 384
BtsIMutI	CAGTG	3 cut(s)	167, 271, 384
Cac8I	GCNNGC	1 cut(s)	280
Csp6I	GTAC	1 cut(s)	88
CviAII	CATG	2 cut(s)	175, 293
CviJI	RGCY	9 cut(s)	68, 80, 114, 159, 179, 278, 351, 425, 439
CviKI_1	RGCY	9 cut(s)	68, 80, 114, 159, 179, 278, 351, 425, 439
CviQI	GTAC	1 cut(s)	88
DdeI	CTNAG	4 cut(s)	115, 144, 319, 335
DpnI	GATC	4 cut(s)	105, 150, 234, 317
DpnII	GATC	4 cut(s)	103, 148, 232, 315
EaeI	YGGCCR	1 cut(s)	157
EagI	CGGCCG	1 cut(s)	157
EclXI	CGGCCG	1 cut(s)	157
Eco147I	AGGCCT	1 cut(s)	68
Eco52I	CGGCCG	1 cut(s)	157
Eco57I	CTGAAG	1 cut(s)	182
FaeI	CATG	2 cut(s)	178, 296
FaiI	YATR	2 cut(s)	176, 294
FalI	AAGNNNNNCTT	2 cut(s)	423, 455
FatI	CATG	2 cut(s)	174, 292
Fnu4HI	GCNGC	3 cut(s)	81, 423, 426
FokI	GGATG	2 cut(s)	410, 419
Fsp4HI	GCNGC	3 cut(s)	81, 423, 426
FspBI	CTAG	2 cut(s)	84, 460
GluI	GCNGC	3 cut(s)	81, 423, 426
GsuI	CTGGAG	3 cut(s)	18, 95, 348
HaeIII	GGCC	2 cut(s)	68, 159
Hin1I	GRCGYC	1 cut(s)	447
Hin1II	CATG	2 cut(s)	178, 296
HindIII	AAGCTT	1 cut(s)	112
HinfI	GANTC	4 cut(s)	29, 119, 140, 361
HphI	GGTGA	2 cut(s)	38, 313
Hpy188I	TCNGA	2 cut(s)	201, 237
Hpy188III	TCNNGA	3 cut(s)	137, 404, 443
HpyCH4IV	ACGT	1 cut(s)	447
HpyCH4V	TGCA	1 cut(s)	172
HpyF3I	CTNAG	4 cut(s)	115, 144, 319, 335
HpySE526I	ACGT	1 cut(s)	447
Hsp92I	GRCGYC	1 cut(s)	447
Hsp92II	CATG	2 cut(s)	178, 296
Kzo9I	GATC	4 cut(s)	103, 148, 232, 315
LmnI	GCTCC	1 cut(s)	326
LpnPI	CCDG	8 cut(s)	48, 59, 259, 296, 309, 370, 378, 389
Lsp1109I	GCAGC	2 cut(s)	67, 412
LweI	GCATC	3 cut(s)	56, 202, 397
MaeI	CTAG	2 cut(s)	84, 460
MaeII	ACGT	1 cut(s)	447
MaeIII	GTNAC	1 cut(s)	443
MalI	GATC	4 cut(s)	105, 150, 234, 317
MboI	GATC	4 cut(s)	103, 148, 232, 315
MboII	GAAGA	1 cut(s)	188
MflI	RGATCY	2 cut(s)	148, 232
MluCI	AATT	1 cut(s)	311
MlyI	GAGTC	1 cut(s)	355
MnlI	CCTC	7 cut(s)	51, 58, 70, 218, 244, 276, 292
MseI	TTAA	4 cut(s)	108, 306, 344, 414
Mva1269I	GAATGC	1 cut(s)	244
NdeII	GATC	4 cut(s)	103, 148, 232, 315
NlaIII	CATG	2 cut(s)	178, 296
NmuCI	GTSAC	1 cut(s)	443
NspI	RCATGY	1 cut(s)	296
PceI	AGGCCT	1 cut(s)	68
PciI	ACATGT	1 cut(s)	292
PctI	GAATGC	1 cut(s)	244
PfeI	GAWTC	3 cut(s)	29, 119, 140
PkrI	GCNGC	3 cut(s)	82, 424, 427
PleI	GAGTC	1 cut(s)	355
PpsI	GAGTC	1 cut(s)	355
PscI	ACATGT	1 cut(s)	292
PsuI	RGATCY	2 cut(s)	148, 232
RsaI	GTAC	1 cut(s)	89
RsaNI	GTAC	1 cut(s)	88
SaqAI	TTAA	4 cut(s)	108, 306, 344, 414
SatI	GCNGC	3 cut(s)	81, 423, 426
Sau3AI	GATC	4 cut(s)	103, 148, 232, 315
SchI	GAGTC	1 cut(s)	355
SetI	ASST	7 cut(s)	89, 116, 268, 303, 353, 441, 450
SfaNI	GCATC	3 cut(s)	56, 202, 397
Sse9I	AATT	1 cut(s)	311
SseBI	AGGCCT	1 cut(s)	68
SsiI	CCGC	1 cut(s)	422
SspMI	CTAG	2 cut(s)	84, 460
StuI	AGGCCT	1 cut(s)	68
TaiI	ACGT	1 cut(s)	450
TaqI	TCGA	1 cut(s)	39
TasI	AATT	1 cut(s)	311
TauI	GCSGC	1 cut(s)	425
TfiI	GAWTC	3 cut(s)	29, 119, 140
Tru1I	TTAA	4 cut(s)	108, 306, 344, 414
Tru9I	TTAA	4 cut(s)	108, 306, 344, 414
TscAI	CASTG	3 cut(s)	174, 278, 384
TseFI	GTSAC	1 cut(s)	443
TseI	GCWGC	2 cut(s)	80, 425
Tsp45I	GTSAC	1 cut(s)	443
TspDTI	ATGAA	2 cut(s)	272, 387
TspRI	CASTG	3 cut(s)	174, 278, 384
XceI	RCATGY	1 cut(s)	296
XspI	CTAG	2 cut(s)	84, 460
ZraI	GACGTC	1 cut(s)	448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.