RchiOBHm_Chr3g0485011

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006700
Physical Location & Seq
Forward (+)
31588304 .. 31591520
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44968

Sequence Viewer

Length: 438 bp
ATGGGACAAGAGGCAACTGCACTTGAGGATTCTCCAGTTGAGTCCTTGCTGCAGGCTGCAAGATATGATGACATGGAAGACATTACGTCGTTAGTATCCGATGGTGTTTCTCTTGATTCTAAGGATTCCCAGGGCCGAACAGCACTACACATGGCTTCTGCTAATGGTCATCTTGACATTGTGGAGTATCTTATCAGTAGAGGAGTGGATCTTAATGCTTCAAATGCGGAGATGAATACACCCCTACATTGGGCCTGCCTGAATGGACATGTCGAGGTGGTTAAGAAATTGATCTTGGCGGGAGCTAATGTAGGTGTACTAAACAGCTATGAGAAGAGCCCAATTGATGAAGCTTTGAGTGGGCAAAAGATGGATGTTATTGATGCCATTAATGAATCAGTGGCCCAAGTAGAGCTTACTGGCATCAGAGTGTCCTAG

Protein Analysis

145

Amino Acids

15.47

Weight (kDa)

4.36

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 16 - 105 2.8e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 17 - 65 7.2e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 31 - 83 1.3e-11 Ankyrin repeats (many copies)
Ank PF00023 44 - 75 6.9e-10 Ankyrin repeat
Ank_3 PF13606 45 - 73 1.4e-07 Ankyrin repeat
Ank_4 PF13637 57 - 98 2e-11 Ankyrin repeats (many copies)
Ank PF00023 79 - 108 1.2e-08 Ankyrin repeat
Ank_3 PF13606 79 - 104 1.7e-06 Ankyrin repeat
Ank_5 PF13857 79 - 118 9.6e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 227, 299
AclWI GGATC 1 cut(s) 216
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 2 cut(s) 249, 250
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 1 cut(s) 222
AhdI GACNNNNNGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 4 cut(s) 305, 327, 353, 415
AluI AGCT 4 cut(s) 305, 327, 353, 415
AlwI GGATC 1 cut(s) 216
AoxI GGCC 3 cut(s) 133, 252, 402
ApeKI GCWGC 2 cut(s) 49, 56
AseI ATTAAT 1 cut(s) 390
AspS9I GGNCC 3 cut(s) 133, 252, 403
BanII GRGCYC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 36, 43
BccI CCATC 2 cut(s) 95, 364
BciT130I CCWGG 1 cut(s) 131
BciVI GTATCC 1 cut(s) 106
BfaI CTAG 1 cut(s) 436
BfmI CTRYAG 1 cut(s) 50
BfuI GTATCC 1 cut(s) 106
BisI GCNGC 2 cut(s) 50, 57
BlsI GCNGC 2 cut(s) 51, 58
Bme1390I CCNGG 1 cut(s) 131
BmeRI GACNNNNNGTC 1 cut(s) 85
BmgT120I GGNCC 3 cut(s) 133, 252, 403
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 373, 432
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 129, 130
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 250
Bse1I ACTGG 2 cut(s) 35, 424
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 129, 130
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 2 cut(s) 249, 250
BseNI ACTGG 2 cut(s) 35, 424
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 36, 43
BshFI GGCC 3 cut(s) 135, 254, 404
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 249, 250
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 3 cut(s) 135, 254, 404
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 2 cut(s) 208, 291
BspACI CCGC 2 cut(s) 227, 299
BspANI GGCC 3 cut(s) 135, 254, 404
BspMAI CTGCAG 1 cut(s) 54
BspPI GGATC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 329
BsrI ACTGG 2 cut(s) 35, 424
BssECI CCNNGG 2 cut(s) 129, 130
BssMI GATC 2 cut(s) 208, 291
Bst2UI CCWGG 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 54, 256
BstDEI CTNAG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 379
BstKTI GATC 2 cut(s) 211, 294
BstMBI GATC 2 cut(s) 208, 291
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 36, 43
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 3 cut(s) 135, 254, 404
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 54, 256
Cfr13I GGNCC 3 cut(s) 133, 252, 403
Csp6I GTAC 1 cut(s) 317
CviAII CATG 3 cut(s) 73, 151, 269
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 1 cut(s) 120
DpnI GATC 2 cut(s) 210, 293
DpnII GATC 2 cut(s) 208, 291
DriI GACNNNNNGTC 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 329
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 341
FaeI CATG 3 cut(s) 76, 154, 272
FaiI YATR 5 cut(s) 66, 74, 152, 270, 330
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 72, 150, 268
FauI CCCGC 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 50, 57
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 50, 57
FspBI CTAG 1 cut(s) 436
GluI GCNGC 2 cut(s) 50, 57
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 3 cut(s) 135, 254, 404
Hin1II CATG 3 cut(s) 76, 154, 272
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 5 cut(s) 29, 41, 116, 125, 395
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 100, 428
Hpy188III TCNNGA 2 cut(s) 113, 173
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 3 cut(s) 20, 52, 59
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 3 cut(s) 76, 154, 272
Kzo9I GATC 2 cut(s) 208, 291
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 7 cut(s) 38, 48, 116, 143, 268, 272, 405
Lsp1109I GCAGC 2 cut(s) 36, 43
LweI GCATC 2 cut(s) 373, 432
MaeI CTAG 1 cut(s) 436
MaeII ACGT 1 cut(s) 86
MalI GATC 2 cut(s) 210, 293
MboI GATC 2 cut(s) 208, 291
MboII GAAGA 2 cut(s) 89, 346
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 2 cut(s) 287, 342
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 4 cut(s) 4, 19, 194, 268
MseI TTAA 3 cut(s) 213, 282, 390
MslI CAYNNNNRTG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 131
MunI CAATTG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 2 cut(s) 208, 291
NlaIII CATG 3 cut(s) 76, 154, 272
NspI RCATGY 1 cut(s) 272
PasI CCCWGGG 1 cut(s) 130
PciI ACATGT 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 29, 116, 125, 395
PkrI GCNGC 2 cut(s) 51, 58
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PscI ACATGT 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspPI GGNCC 3 cut(s) 133, 252, 403
PstI CTGCAG 1 cut(s) 54
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 428
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 213, 282, 390
SatI GCNGC 2 cut(s) 50, 57
Sau3AI GATC 2 cut(s) 208, 291
Sau96I GGNCC 3 cut(s) 133, 252, 403
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 341
SetI ASST 7 cut(s) 89, 279, 307, 316, 329, 355, 417
SfaNI GCATC 2 cut(s) 373, 432
SfcI CTRYAG 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 2 cut(s) 287, 342
SsiI CCGC 2 cut(s) 227, 299
SspMI CTAG 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 129
TaiI ACGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 273
TasI AATT 2 cut(s) 287, 342
TatI WGTACW 1 cut(s) 316
TfiI GAWTC 4 cut(s) 29, 116, 125, 395
Tru1I TTAA 3 cut(s) 213, 282, 390
Tru9I TTAA 3 cut(s) 213, 282, 390
TscAI CASTG 1 cut(s) 405
TseI GCWGC 2 cut(s) 49, 56
TspDTI ATGAA 3 cut(s) 248, 363, 408
TspRI CASTG 1 cut(s) 405
VspI ATTAAT 1 cut(s) 390
XceI RCATGY 1 cut(s) 272
XspI CTAG 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.