Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 125 |
| AatII | GACGTC | 1 cut(s) | 123 |
| AccB1I | GGYRCC | 1 cut(s) | 203 |
| AccBSI | CCGCTC | 1 cut(s) | 1219 |
| AccI | GTMKAC | 1 cut(s) | 790 |
| AccII | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| AccIII | TCCGGA | 1 cut(s) | 1420 |
| AclI | AACGTT | 1 cut(s) | 293 |
| AclWI | GGATC | 3 cut(s) | 493, 506, 1556 |
| AcsI | RAATTY | 1 cut(s) | 630 |
| AcyI | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| AfaI | GTAC | 1 cut(s) | 892 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1209, 1420 |
| AflIII | ACRYGT | 1 cut(s) | 1259 |
| AgsI | TTSAA | 5 cut(s) | 758, 782, 833, 1351, 1569 |
| AjnI | CCWGG | 1 cut(s) | 1202 |
| AluBI | AGCT | 8 cut(s) | 200, 529, 659, 722, 785, 853, 1064, 1325 |
| AluI | AGCT | 8 cut(s) | 200, 529, 659, 722, 785, 853, 1064, 1325 |
| Alw26I | GTCTC | 7 cut(s) | 133, 668, 710, 1018, 1235, 1250, 1418 |
| AlwI | GGATC | 3 cut(s) | 493, 506, 1556 |
| Ama87I | CYCGRG | 1 cut(s) | 1220 |
| Aor13HI | TCCGGA | 1 cut(s) | 1420 |
| AoxI | GGCC | 1 cut(s) | 100 |
| ApeKI | GCWGC | 2 cut(s) | 89, 1193 |
| ApoI | RAATTY | 1 cut(s) | 630 |
| AspLEI | GCGC | 3 cut(s) | 206, 640, 922 |
| AspS9I | GGNCC | 3 cut(s) | 576, 744, 1214 |
| AsuHPI | GGTGA | 3 cut(s) | 679, 991, 1541 |
| AvaI | CYCGRG | 1 cut(s) | 1220 |
| AvaII | GGWCC | 3 cut(s) | 576, 744, 1214 |
| BamHI | GGATCC | 1 cut(s) | 498 |
| BanI | GGYRCC | 1 cut(s) | 203 |
| BanII | GRGCYC | 1 cut(s) | 394 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 943, 975 |
| BauI | CACGAG | 1 cut(s) | 1514 |
| BbvCI | CCTCAGC | 1 cut(s) | 849 |
| BbvI | GCAGC | 2 cut(s) | 76, 1180 |
| BccI | CCATC | 1 cut(s) | 597 |
| BceAI | ACGGC | 2 cut(s) | 446, 848 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1171, 1205 |
| BciT130I | CCWGG | 1 cut(s) | 1204 |
| BciVI | GTATCC | 1 cut(s) | 557 |
| BclI | TGATCA | 2 cut(s) | 1501, 1510 |
| BcoDI | GTCTC | 7 cut(s) | 133, 668, 710, 1018, 1235, 1250, 1418 |
| BfaI | CTAG | 8 cut(s) | 401, 495, 786, 1058, 1163, 1236, 1253, 1488 |
| BfoI | RGCGCY | 1 cut(s) | 207 |
| BfuI | GTATCC | 1 cut(s) | 557 |
| BisI | GCNGC | 3 cut(s) | 90, 100, 1194 |
| BlsI | GCNGC | 3 cut(s) | 91, 101, 1195 |
| Bme1390I | CCNGG | 1 cut(s) | 1204 |
| Bme18I | GGWCC | 3 cut(s) | 576, 744, 1214 |
| BmeT110I | CYCGRG | 1 cut(s) | 1220 |
| BmgT120I | GGNCC | 3 cut(s) | 576, 744, 1214 |
| BmiI | GGNNCC | 5 cut(s) | 205, 500, 577, 745, 1215 |
| BmrFI | CCNGG | 1 cut(s) | 1204 |
| BmsI | GCATC | 2 cut(s) | 90, 138 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 690, 722, 1038, 1070 |
| Bpu10I | CCTNAGC | 1 cut(s) | 849 |
| BpuEI | CTTGAG | 3 cut(s) | 378, 1071, 1305 |
| Bsa29I | ATCGAT | 1 cut(s) | 146 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1364 |
| BsaHI | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| BsaJI | CCNNGG | 5 cut(s) | 177, 241, 502, 1108, 1266 |
| BsaWI | WCCGGW | 3 cut(s) | 5, 983, 1420 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1209, 1420 |
| Bse118I | RCCGGY | 3 cut(s) | 95, 278, 350 |
| Bse1I | ACTGG | 1 cut(s) | 898 |
| Bse3DI | GCAATG | 1 cut(s) | 893 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1364 |
| BseAI | TCCGGA | 1 cut(s) | 1420 |
| BseBI | CCWGG | 1 cut(s) | 1204 |
| BseCI | ATCGAT | 1 cut(s) | 146 |
| BseDI | CCNNGG | 5 cut(s) | 177, 241, 502, 1108, 1266 |
| BseGI | GGATG | 2 cut(s) | 608, 1504 |
| BseJI | GATNNNNATC | 1 cut(s) | 1364 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1209, 1420 |
| BseMI | GCAATG | 1 cut(s) | 893 |
| BseMII | CTCAG | 3 cut(s) | 863, 984, 1446 |
| BseNI | ACTGG | 1 cut(s) | 898 |
| BseRI | GAGGAG | 2 cut(s) | 24, 1460 |
| BseXI | GCAGC | 2 cut(s) | 76, 1180 |
| BsgI | GTGCAG | 1 cut(s) | 1165 |
| Bsh1236I | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| Bsh1285I | CGRYCG | 2 cut(s) | 654, 1042 |
| BshFI | GGCC | 1 cut(s) | 102 |
| BshNI | GGYRCC | 1 cut(s) | 203 |
| BshVI | ATCGAT | 1 cut(s) | 146 |
| BsiEI | CGRYCG | 2 cut(s) | 654, 1042 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1220 |
| BsiSI | CCGG | 8 cut(s) | 6, 96, 132, 152, 279, 351, 984, 1421 |
| BslFI | GGGAC | 4 cut(s) | 589, 757, 1227, 1284 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1209, 1420 |
| BsmAI | GTCTC | 7 cut(s) | 133, 668, 710, 1018, 1235, 1250, 1418 |
| BsmBI | CGTCTC | 1 cut(s) | 710 |
| BsmFI | GGGAC | 4 cut(s) | 589, 757, 1227, 1284 |
| BsnI | GGCC | 1 cut(s) | 102 |
| BsoBI | CYCGRG | 1 cut(s) | 1220 |
| Bsp1286I | GDGCHC | 1 cut(s) | 394 |
| Bsp13I | TCCGGA | 1 cut(s) | 1420 |
| Bsp143I | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| BspANI | GGCC | 1 cut(s) | 102 |
| BspCNI | CTCAG | 3 cut(s) | 862, 983, 1447 |
| BspDI | ATCGAT | 1 cut(s) | 146 |
| BspEI | TCCGGA | 1 cut(s) | 1420 |
| BspFNI | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| BspLI | GGNNCC | 5 cut(s) | 205, 500, 577, 745, 1215 |
| BspPI | GGATC | 3 cut(s) | 493, 506, 1556 |
| BspQI | GCTCTTC | 1 cut(s) | 1307 |
| BspT107I | GGYRCC | 1 cut(s) | 203 |
| BsrBI | CCGCTC | 1 cut(s) | 1219 |
| BsrDI | GCAATG | 1 cut(s) | 893 |
| BsrFI | RCCGGY | 3 cut(s) | 95, 278, 350 |
| BsrI | ACTGG | 1 cut(s) | 898 |
| BssAI | RCCGGY | 3 cut(s) | 95, 278, 350 |
| BssECI | CCNNGG | 5 cut(s) | 177, 241, 502, 1108, 1266 |
| BssMI | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| BssNI | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| BssSI | CACGAG | 1 cut(s) | 1514 |
| BssT1I | CCWWGG | 2 cut(s) | 241, 1108 |
| Bst2BI | CACGAG | 1 cut(s) | 1514 |
| Bst2UI | CCWGG | 1 cut(s) | 1204 |
| Bst4CI | ACNGT | 4 cut(s) | 652, 686, 845, 1285 |
| Bst6I | CTCTTC | 3 cut(s) | 557, 1307, 1529 |
| BstACI | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 225 |
| BstC8I | GCNNGC | 3 cut(s) | 202, 352, 418 |
| BstDEI | CTNAG | 5 cut(s) | 274, 849, 970, 1428, 1455 |
| BstDSI | CCRYGG | 3 cut(s) | 177, 502, 1266 |
| BstEII | GGTNACC | 1 cut(s) | 979 |
| BstF5I | GGATG | 2 cut(s) | 608, 1504 |
| BstFNI | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| BstH2I | RGCGCY | 1 cut(s) | 207 |
| BstHHI | GCGC | 3 cut(s) | 206, 640, 922 |
| BstKTI | GATC | 6 cut(s) | 501, 775, 1179, 1504, 1513, 1564 |
| BstMAI | GTCTC | 7 cut(s) | 133, 668, 710, 1018, 1235, 1250, 1418 |
| BstMBI | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| BstMCI | CGRYCG | 2 cut(s) | 654, 1042 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 26, 225, 340, 383, 458, 1199 |
| BstNI | CCWGG | 1 cut(s) | 1204 |
| BstPI | GGTNACC | 1 cut(s) | 979 |
| BstSCI | CCNGG | 1 cut(s) | 1202 |
| BstUI | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| BstV1I | GCAGC | 2 cut(s) | 76, 1180 |
| BstX2I | RGATCY | 1 cut(s) | 498 |
| BstYI | RGATCY | 1 cut(s) | 498 |
| Bsu15I | ATCGAT | 1 cut(s) | 146 |
| BsuI | GTATCC | 1 cut(s) | 557 |
| BsuRI | GGCC | 1 cut(s) | 102 |
| BsuTUI | ATCGAT | 1 cut(s) | 146 |
| BtgI | CCRYGG | 3 cut(s) | 177, 502, 1266 |
| BtgZI | GCGATG | 1 cut(s) | 844 |
| BtsCI | GGATG | 2 cut(s) | 608, 1504 |
| BtsI | GCAGTG | 1 cut(s) | 981 |
| BtsIMutI | CAGTG | 1 cut(s) | 981 |
| Cac8I | GCNNGC | 3 cut(s) | 202, 352, 418 |
| CfoI | GCGC | 3 cut(s) | 206, 640, 922 |
| Cfr10I | RCCGGY | 3 cut(s) | 95, 278, 350 |
| Cfr13I | GGNCC | 3 cut(s) | 576, 744, 1214 |
| Cfr42I | CCGCGG | 1 cut(s) | 1269 |
| ClaI | ATCGAT | 1 cut(s) | 146 |
| CseI | GACGC | 2 cut(s) | 965, 1102 |
| Csp6I | GTAC | 1 cut(s) | 891 |
| CviAII | CATG | 5 cut(s) | 85, 106, 406, 1380, 1495 |
| CviQI | GTAC | 1 cut(s) | 891 |
| DdeI | CTNAG | 5 cut(s) | 274, 849, 970, 1428, 1455 |
| DinI | GGCGCC | 1 cut(s) | 205 |
| DpnI | GATC | 6 cut(s) | 500, 774, 1178, 1503, 1512, 1563 |
| DpnII | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 125 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 125 |
| Eam1104I | CTCTTC | 3 cut(s) | 557, 1307, 1529 |
| EarI | CTCTTC | 3 cut(s) | 557, 1307, 1529 |
| EciI | GGCGGA | 1 cut(s) | 150 |
| Eco130I | CCWWGG | 2 cut(s) | 241, 1108 |
| Eco24I | GRGCYC | 1 cut(s) | 394 |
| Eco47I | GGWCC | 3 cut(s) | 576, 744, 1214 |
| Eco88I | CYCGRG | 1 cut(s) | 1220 |
| Eco91I | GGTNACC | 1 cut(s) | 979 |
| EcoO65I | GGTNACC | 1 cut(s) | 979 |
| EcoRI | GAATTC | 1 cut(s) | 630 |
| EcoRII | CCWGG | 1 cut(s) | 1202 |
| EcoT14I | CCWWGG | 2 cut(s) | 241, 1108 |
| EcoT38I | GRGCYC | 1 cut(s) | 394 |
| EgeI | GGCGCC | 1 cut(s) | 205 |
| EheI | GGCGCC | 1 cut(s) | 205 |
| ErhI | CCWWGG | 2 cut(s) | 241, 1108 |
| Esp3I | CGTCTC | 1 cut(s) | 710 |
| FaeI | CATG | 5 cut(s) | 88, 109, 409, 1383, 1498 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 711, 743 |
| FaqI | GGGAC | 4 cut(s) | 589, 757, 1227, 1284 |
| FatI | CATG | 5 cut(s) | 84, 105, 405, 1379, 1494 |
| FauI | CCCGC | 4 cut(s) | 379, 619, 643, 1261 |
| FbaI | TGATCA | 2 cut(s) | 1501, 1510 |
| FblI | GTMKAC | 1 cut(s) | 790 |
| Fnu4HI | GCNGC | 3 cut(s) | 90, 100, 1194 |
| FokI | GGATG | 2 cut(s) | 615, 1511 |
| FriOI | GRGCYC | 1 cut(s) | 394 |
| Fsp4HI | GCNGC | 3 cut(s) | 90, 100, 1194 |
| FspBI | CTAG | 8 cut(s) | 401, 495, 786, 1058, 1163, 1236, 1253, 1488 |
| GlaI | GCGC | 3 cut(s) | 205, 639, 921 |
| GluI | GCNGC | 3 cut(s) | 90, 100, 1194 |
| HaeII | RGCGCY | 1 cut(s) | 207 |
| HaeIII | GGCC | 1 cut(s) | 102 |
| HapII | CCGG | 8 cut(s) | 6, 96, 132, 152, 279, 351, 984, 1421 |
| HgaI | GACGC | 2 cut(s) | 965, 1102 |
| HhaI | GCGC | 3 cut(s) | 206, 640, 922 |
| Hin1I | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| Hin1II | CATG | 5 cut(s) | 88, 109, 409, 1383, 1498 |
| Hin6I | GCGC | 3 cut(s) | 204, 638, 920 |
| HinP1I | GCGC | 3 cut(s) | 204, 638, 920 |
| HincII | GTYRAC | 5 cut(s) | 568, 791, 994, 1030, 1294 |
| HindII | GTYRAC | 5 cut(s) | 568, 791, 994, 1030, 1294 |
| HindIII | AAGCTT | 1 cut(s) | 1323 |
| HinfI | GANTC | 1 cut(s) | 897 |
| HpaI | GTTAAC | 1 cut(s) | 1294 |
| HpaII | CCGG | 8 cut(s) | 6, 96, 132, 152, 279, 351, 984, 1421 |
| HphI | GGTGA | 3 cut(s) | 679, 991, 1541 |
| Hpy166II | GTNNAC | 5 cut(s) | 568, 791, 994, 1030, 1294 |
| Hpy188I | TCNGA | 4 cut(s) | 189, 326, 481, 656 |
| Hpy188III | TCNNGA | 7 cut(s) | 161, 395, 707, 1253, 1421, 1514, 1522 |
| Hpy8I | GTNNAC | 5 cut(s) | 568, 791, 994, 1030, 1294 |
| Hpy99I | CGWCG | 5 cut(s) | 122, 125, 735, 869, 1193 |
| HpyAV | CCTTC | 4 cut(s) | 321, 707, 836, 1575 |
| HpyCH4III | ACNGT | 4 cut(s) | 652, 686, 845, 1285 |
| HpyCH4IV | ACGT | 8 cut(s) | 120, 293, 534, 835, 996, 1188, 1261, 1307 |
| HpyCH4V | TGCA | 6 cut(s) | 81, 228, 416, 488, 1182, 1379 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 26, 225, 340, 383, 458, 1199 |
| HpyF3I | CTNAG | 5 cut(s) | 274, 849, 970, 1428, 1455 |
| HpySE526I | ACGT | 8 cut(s) | 120, 293, 534, 835, 996, 1188, 1261, 1307 |
| Hsp92I | GRCGYC | 3 cut(s) | 120, 204, 1113 |
| Hsp92II | CATG | 5 cut(s) | 88, 109, 409, 1383, 1498 |
| HspAI | GCGC | 3 cut(s) | 204, 638, 920 |
| KasI | GGCGCC | 1 cut(s) | 203 |
| Kpn2I | TCCGGA | 1 cut(s) | 1420 |
| KroI | GCCGGC | 1 cut(s) | 350 |
| KroNI | GCCGGC | 1 cut(s) | 352 |
| Ksp22I | TGATCA | 2 cut(s) | 1501, 1510 |
| KspAI | GTTAAC | 1 cut(s) | 1294 |
| KspI | CCGCGG | 1 cut(s) | 1269 |
| Kzo9I | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| LguI | GCTCTTC | 1 cut(s) | 1307 |
| LmnI | GCTCC | 2 cut(s) | 656, 986 |
| Lsp1109I | GCAGC | 2 cut(s) | 76, 1180 |
| LweI | GCATC | 2 cut(s) | 90, 138 |
| MaeI | CTAG | 8 cut(s) | 401, 495, 786, 1058, 1163, 1236, 1253, 1488 |
| MaeII | ACGT | 8 cut(s) | 120, 293, 534, 835, 996, 1188, 1261, 1307 |
| MaeIII | GTNAC | 8 cut(s) | 113, 139, 535, 667, 953, 979, 1120, 1279 |
| MalI | GATC | 6 cut(s) | 500, 774, 1178, 1503, 1512, 1563 |
| MbiI | CCGCTC | 1 cut(s) | 1219 |
| MboI | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| MflI | RGATCY | 1 cut(s) | 498 |
| MhlI | GDGCHC | 1 cut(s) | 394 |
| MluCI | AATT | 2 cut(s) | 630, 1158 |
| Mly113I | GGCGCC | 1 cut(s) | 204 |
| MmeI | TCCRAC | 2 cut(s) | 459, 634 |
| MroI | TCCGGA | 1 cut(s) | 1420 |
| MroNI | GCCGGC | 1 cut(s) | 350 |
| MseI | TTAA | 7 cut(s) | 513, 531, 588, 807, 1229, 1293, 1579 |
| MslI | CAYNNNNRTG | 1 cut(s) | 371 |
| MspA1I | CMGCKG | 1 cut(s) | 1268 |
| MspI | CCGG | 8 cut(s) | 6, 96, 132, 152, 279, 351, 984, 1421 |
| MspR9I | CCNGG | 1 cut(s) | 1204 |
| MvaI | CCWGG | 1 cut(s) | 1204 |
| MvnI | CGCG | 5 cut(s) | 386, 638, 905, 920, 1268 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 26, 225, 340, 383, 458, 1199 |
| NaeI | GCCGGC | 1 cut(s) | 352 |
| NarI | GGCGCC | 1 cut(s) | 204 |
| NdeII | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| NgoMIV | GCCGGC | 1 cut(s) | 350 |
| NlaIII | CATG | 5 cut(s) | 88, 109, 409, 1383, 1498 |
| NlaIV | GGNNCC | 5 cut(s) | 205, 500, 577, 745, 1215 |
| NmeAIII | GCCGAG | 1 cut(s) | 1070 |
| NmuCI | GTSAC | 5 cut(s) | 139, 535, 667, 953, 979 |
| PciSI | GCTCTTC | 1 cut(s) | 1307 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 736, 841, 861 |
| PdiI | GCCGGC | 1 cut(s) | 352 |
| PfeI | GAWTC | 1 cut(s) | 897 |
| PflFI | GACNNNGTC | 1 cut(s) | 1034 |
| PkrI | GCNGC | 3 cut(s) | 91, 101, 1195 |
| PluTI | GGCGCC | 1 cut(s) | 207 |
| Psp1406I | AACGTT | 1 cut(s) | 293 |
| Psp6I | CCWGG | 1 cut(s) | 1202 |
| PspEI | GGTNACC | 1 cut(s) | 979 |
| PspGI | CCWGG | 1 cut(s) | 1202 |
| PspN4I | GGNNCC | 5 cut(s) | 205, 500, 577, 745, 1215 |
| PspPI | GGNCC | 3 cut(s) | 576, 744, 1214 |
| PsuI | RGATCY | 1 cut(s) | 498 |
| PsyI | GACNNNGTC | 1 cut(s) | 1034 |
| RsaI | GTAC | 1 cut(s) | 892 |
| RsaNI | GTAC | 1 cut(s) | 891 |
| RseI | CAYNNNNRTG | 1 cut(s) | 371 |
| SacII | CCGCGG | 1 cut(s) | 1269 |
| SalI | GTCGAC | 1 cut(s) | 789 |
| SapI | GCTCTTC | 1 cut(s) | 1307 |
| SaqAI | TTAA | 7 cut(s) | 513, 531, 588, 807, 1229, 1293, 1579 |
| SatI | GCNGC | 3 cut(s) | 90, 100, 1194 |
| Sau3AI | GATC | 6 cut(s) | 498, 772, 1176, 1501, 1510, 1561 |
| Sau96I | GGNCC | 3 cut(s) | 576, 744, 1214 |
| ScrFI | CCNGG | 1 cut(s) | 1204 |
| SduI | GDGCHC | 1 cut(s) | 394 |
| SfaNI | GCATC | 2 cut(s) | 90, 138 |
| SfoI | GGCGCC | 1 cut(s) | 205 |
| Sfr303I | CCGCGG | 1 cut(s) | 1269 |
| SgrBI | CCGCGG | 1 cut(s) | 1269 |
| SinI | GGWCC | 3 cut(s) | 576, 744, 1214 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 371 |
| SmlI | CTYRAG | 3 cut(s) | 393, 1086, 1320 |
| SmoI | CTYRAG | 3 cut(s) | 393, 1086, 1320 |
| Sse9I | AATT | 2 cut(s) | 630, 1158 |
| SspDI | GGCGCC | 1 cut(s) | 203 |
| SspI | AATATT | 2 cut(s) | 517, 1435 |
| SspMI | CTAG | 8 cut(s) | 401, 495, 786, 1058, 1163, 1236, 1253, 1488 |
| StyD4I | CCNGG | 1 cut(s) | 1202 |
| StyI | CCWWGG | 2 cut(s) | 241, 1108 |
| TaaI | ACNGT | 4 cut(s) | 652, 686, 845, 1285 |
| TaiI | ACGT | 8 cut(s) | 123, 296, 537, 838, 999, 1191, 1264, 1310 |
| TaqI | TCGA | 7 cut(s) | 146, 162, 693, 708, 739, 790, 1443 |
| TaqII | GACCGA | 2 cut(s) | 761, 1028 |
| TasI | AATT | 2 cut(s) | 630, 1158 |
| TatI | WGTACW | 1 cut(s) | 890 |
| TauI | GCSGC | 1 cut(s) | 102 |
| TfiI | GAWTC | 1 cut(s) | 897 |
| Tru1I | TTAA | 7 cut(s) | 513, 531, 588, 807, 1229, 1293, 1579 |
| Tru9I | TTAA | 7 cut(s) | 513, 531, 588, 807, 1229, 1293, 1579 |
| TscAI | CASTG | 1 cut(s) | 981 |
| TseFI | GTSAC | 5 cut(s) | 139, 535, 667, 953, 979 |
| TseI | GCWGC | 2 cut(s) | 89, 1193 |
| Tsp45I | GTSAC | 5 cut(s) | 139, 535, 667, 953, 979 |
| TspDTI | ATGAA | 6 cut(s) | 294, 352, 726, 1300, 1354, 1483 |
| TspGWI | ACGGA | 4 cut(s) | 48, 456, 491, 1015 |
| TspRI | CASTG | 1 cut(s) | 981 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1034 |
| VpaK11BI | GGWCC | 3 cut(s) | 576, 744, 1214 |
| XapI | RAATTY | 1 cut(s) | 630 |
| XbaI | TCTAGA | 1 cut(s) | 1252 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 890, 1352 |
| XmiI | GTMKAC | 1 cut(s) | 790 |
| XspI | CTAG | 8 cut(s) | 401, 495, 786, 1058, 1163, 1236, 1253, 1488 |
| ZraI | GACGTC | 1 cut(s) | 121 |