Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 56 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 973 |
| AccBSI | CCGCTC | 1 cut(s) | 132 |
| AccI | GTMKAC | 1 cut(s) | 705 |
| AccII | CGCG | 1 cut(s) | 774 |
| AciI | CCGC | 6 cut(s) | 44, 132, 312, 420, 772, 774 |
| AclWI | GGATC | 1 cut(s) | 1258 |
| AcoI | YGGCCR | 1 cut(s) | 1303 |
| AcsI | RAATTY | 5 cut(s) | 77, 162, 203, 531, 1191 |
| AcyI | GRCGYC | 1 cut(s) | 57 |
| AfaI | GTAC | 4 cut(s) | 305, 341, 826, 1514 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 973 |
| AgsI | TTSAA | 8 cut(s) | 32, 83, 199, 791, 1106, 1289, 1368, 1541 |
| AjnI | CCWGG | 2 cut(s) | 238, 489 |
| AluBI | AGCT | 8 cut(s) | 383, 410, 562, 659, 1070, 1097, 1185, 1412 |
| AluI | AGCT | 8 cut(s) | 383, 410, 562, 659, 1070, 1097, 1185, 1412 |
| Alw21I | GWGCWC | 1 cut(s) | 544 |
| Alw26I | GTCTC | 3 cut(s) | 696, 1268, 1451 |
| AlwI | GGATC | 1 cut(s) | 1258 |
| AoxI | GGCC | 3 cut(s) | 136, 878, 1303 |
| ApeKI | GCWGC | 2 cut(s) | 125, 422 |
| ApoI | RAATTY | 5 cut(s) | 77, 162, 203, 531, 1191 |
| AseI | ATTAAT | 1 cut(s) | 933 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 203 |
| AspLEI | GCGC | 1 cut(s) | 59 |
| AspS9I | GGNCC | 3 cut(s) | 1247, 1417, 1547 |
| AsuHPI | GGTGA | 3 cut(s) | 25, 379, 562 |
| AvaII | GGWCC | 3 cut(s) | 1247, 1417, 1547 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1029, 1062 |
| BalI | TGGCCA | 1 cut(s) | 1305 |
| BanI | GGYRCC | 1 cut(s) | 56 |
| Bbv12I | GWGCWC | 1 cut(s) | 544 |
| BbvI | GCAGC | 2 cut(s) | 137, 434 |
| BccI | CCATC | 3 cut(s) | 1448, 1526, 1538 |
| BceAI | ACGGC | 2 cut(s) | 843, 1118 |
| BciT130I | CCWGG | 2 cut(s) | 240, 491 |
| BclI | TGATCA | 2 cut(s) | 105, 597 |
| BcoDI | GTCTC | 3 cut(s) | 696, 1268, 1451 |
| BfaI | CTAG | 4 cut(s) | 356, 1196, 1253, 1269 |
| BfoI | RGCGCY | 1 cut(s) | 60 |
| BisI | GCNGC | 4 cut(s) | 126, 312, 420, 423 |
| BlsI | GCNGC | 4 cut(s) | 127, 313, 421, 424 |
| Bme1390I | CCNGG | 2 cut(s) | 240, 491 |
| Bme18I | GGWCC | 3 cut(s) | 1247, 1417, 1547 |
| BmgT120I | GGNCC | 3 cut(s) | 1247, 1417, 1547 |
| BmiI | GGNNCC | 4 cut(s) | 58, 666, 1248, 1418 |
| BmrFI | CCNGG | 2 cut(s) | 240, 491 |
| BmrI | ACTGGG | 1 cut(s) | 659 |
| BmsI | GCATC | 3 cut(s) | 344, 894, 979 |
| BmuI | ACTGGG | 1 cut(s) | 659 |
| BpmI | CTGGAG | 1 cut(s) | 939 |
| BsaHI | GRCGYC | 1 cut(s) | 57 |
| BsaI | GGTCTC | 2 cut(s) | 696, 1451 |
| BsaJI | CCNNGG | 2 cut(s) | 772, 1174 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1119, 1149 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 973 |
| Bse1I | ACTGG | 3 cut(s) | 654, 956, 1520 |
| Bse3DI | GCAATG | 1 cut(s) | 432 |
| BseBI | CCWGG | 2 cut(s) | 240, 491 |
| BseDI | CCNNGG | 2 cut(s) | 772, 1174 |
| BseGI | GGATG | 2 cut(s) | 1004, 1537 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 973 |
| BseMI | GCAATG | 1 cut(s) | 432 |
| BseMII | CTCAG | 1 cut(s) | 1446 |
| BseNI | ACTGG | 3 cut(s) | 654, 956, 1520 |
| BseXI | GCAGC | 2 cut(s) | 137, 434 |
| BseYI | CCCAGC | 3 cut(s) | 383, 659, 1097 |
| Bsh1236I | CGCG | 1 cut(s) | 774 |
| BshFI | GGCC | 3 cut(s) | 138, 880, 1305 |
| BshNI | GGYRCC | 1 cut(s) | 56 |
| BsiHKAI | GWGCWC | 1 cut(s) | 544 |
| BsiSI | CCGG | 1 cut(s) | 668 |
| BsiWI | CGTACG | 1 cut(s) | 824 |
| BslFI | GGGAC | 1 cut(s) | 1158 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 973 |
| BsmAI | GTCTC | 3 cut(s) | 696, 1268, 1451 |
| BsmFI | GGGAC | 1 cut(s) | 1158 |
| BsmI | GAATGC | 2 cut(s) | 579, 1086 |
| BsnI | GGCC | 3 cut(s) | 138, 880, 1305 |
| Bso31I | GGTCTC | 2 cut(s) | 696, 1451 |
| Bsp1286I | GDGCHC | 1 cut(s) | 544 |
| Bsp143I | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| BspACI | CCGC | 6 cut(s) | 44, 132, 312, 420, 772, 774 |
| BspANI | GGCC | 3 cut(s) | 138, 880, 1305 |
| BspCNI | CTCAG | 1 cut(s) | 1447 |
| BspFNI | CGCG | 1 cut(s) | 774 |
| BspHI | TCATGA | 1 cut(s) | 1425 |
| BspLI | GGNNCC | 4 cut(s) | 58, 666, 1248, 1418 |
| BspPI | GGATC | 1 cut(s) | 1258 |
| BspQI | GCTCTTC | 1 cut(s) | 1340 |
| BspT107I | GGYRCC | 1 cut(s) | 56 |
| BspTNI | GGTCTC | 2 cut(s) | 696, 1451 |
| BsrBI | CCGCTC | 1 cut(s) | 132 |
| BsrDI | GCAATG | 1 cut(s) | 432 |
| BsrI | ACTGG | 3 cut(s) | 654, 956, 1520 |
| BssECI | CCNNGG | 2 cut(s) | 772, 1174 |
| BssMI | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| BssNI | GRCGYC | 1 cut(s) | 57 |
| Bst2UI | CCWGG | 2 cut(s) | 240, 491 |
| Bst4CI | ACNGT | 5 cut(s) | 217, 260, 685, 719, 1042 |
| Bst6I | CTCTTC | 3 cut(s) | 560, 1340, 1514 |
| BstACI | GRCGYC | 1 cut(s) | 57 |
| BstC8I | GCNNGC | 3 cut(s) | 130, 1294, 1303 |
| BstDEI | CTNAG | 3 cut(s) | 7, 761, 1455 |
| BstDSI | CCRYGG | 1 cut(s) | 772 |
| BstEII | GGTNACC | 1 cut(s) | 367 |
| BstF5I | GGATG | 2 cut(s) | 1004, 1537 |
| BstFNI | CGCG | 1 cut(s) | 774 |
| BstH2I | RGCGCY | 1 cut(s) | 60 |
| BstHHI | GCGC | 1 cut(s) | 59 |
| BstKTI | GATC | 5 cut(s) | 93, 108, 600, 726, 1266 |
| BstMAI | GTCTC | 3 cut(s) | 696, 1268, 1451 |
| BstMBI | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 320, 416, 491, 1232, 1289, 1298, 1302 |
| BstNI | CCWGG | 2 cut(s) | 240, 491 |
| BstNSI | RCATGY | 1 cut(s) | 1333 |
| BstPI | GGTNACC | 1 cut(s) | 367 |
| BstSCI | CCNGG | 2 cut(s) | 238, 489 |
| BstUI | CGCG | 1 cut(s) | 774 |
| BstV1I | GCAGC | 2 cut(s) | 137, 434 |
| BsuRI | GGCC | 3 cut(s) | 138, 880, 1305 |
| BtgI | CCRYGG | 1 cut(s) | 772 |
| BtsCI | GGATG | 2 cut(s) | 1004, 1537 |
| BtsIMutI | CAGTG | 1 cut(s) | 647 |
| Cac8I | GCNNGC | 3 cut(s) | 130, 1294, 1303 |
| CciI | TCATGA | 1 cut(s) | 1425 |
| CfoI | GCGC | 1 cut(s) | 59 |
| Cfr13I | GGNCC | 3 cut(s) | 1247, 1417, 1547 |
| Cfr42I | CCGCGG | 1 cut(s) | 775 |
| Csp6I | GTAC | 4 cut(s) | 304, 340, 825, 1513 |
| CviAII | CATG | 8 cut(s) | 40, 373, 439, 943, 1236, 1330, 1381, 1426 |
| CviQI | GTAC | 4 cut(s) | 304, 340, 825, 1513 |
| DdeI | CTNAG | 3 cut(s) | 7, 761, 1455 |
| DinI | GGCGCC | 1 cut(s) | 58 |
| DpnI | GATC | 5 cut(s) | 92, 107, 599, 725, 1265 |
| DpnII | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| EaeI | YGGCCR | 1 cut(s) | 1303 |
| Eam1104I | CTCTTC | 3 cut(s) | 560, 1340, 1514 |
| EarI | CTCTTC | 3 cut(s) | 560, 1340, 1514 |
| Eco147I | AGGCCT | 2 cut(s) | 138, 880 |
| Eco31I | GGTCTC | 2 cut(s) | 696, 1451 |
| Eco47I | GGWCC | 3 cut(s) | 1247, 1417, 1547 |
| Eco91I | GGTNACC | 1 cut(s) | 367 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1247, 1417 |
| EcoO65I | GGTNACC | 1 cut(s) | 367 |
| EcoRI | GAATTC | 2 cut(s) | 531, 1191 |
| EcoRII | CCWGG | 2 cut(s) | 238, 489 |
| EgeI | GGCGCC | 1 cut(s) | 58 |
| EheI | GGCGCC | 1 cut(s) | 58 |
| FaeI | CATG | 8 cut(s) | 43, 376, 442, 946, 1239, 1333, 1384, 1429 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1347, 1379 |
| FaqI | GGGAC | 1 cut(s) | 1158 |
| FatI | CATG | 8 cut(s) | 39, 372, 438, 942, 1235, 1329, 1380, 1425 |
| FauI | CCCGC | 1 cut(s) | 37 |
| FbaI | TGATCA | 2 cut(s) | 105, 597 |
| FblI | GTMKAC | 1 cut(s) | 705 |
| Fnu4HI | GCNGC | 4 cut(s) | 126, 312, 420, 423 |
| FokI | GGATG | 2 cut(s) | 991, 1544 |
| Fsp4HI | GCNGC | 4 cut(s) | 126, 312, 420, 423 |
| FspBI | CTAG | 4 cut(s) | 356, 1196, 1253, 1269 |
| GlaI | GCGC | 1 cut(s) | 58 |
| GluI | GCNGC | 4 cut(s) | 126, 312, 420, 423 |
| GsaI | CCCAGC | 3 cut(s) | 387, 663, 1101 |
| GsuI | CTGGAG | 1 cut(s) | 939 |
| HaeII | RGCGCY | 1 cut(s) | 60 |
| HaeIII | GGCC | 3 cut(s) | 138, 880, 1305 |
| HapII | CCGG | 1 cut(s) | 668 |
| HhaI | GCGC | 1 cut(s) | 59 |
| Hin1I | GRCGYC | 1 cut(s) | 57 |
| Hin1II | CATG | 8 cut(s) | 43, 376, 442, 946, 1239, 1333, 1384, 1429 |
| Hin6I | GCGC | 1 cut(s) | 57 |
| HinP1I | GCGC | 1 cut(s) | 57 |
| HincII | GTYRAC | 1 cut(s) | 781 |
| HindII | GTYRAC | 1 cut(s) | 781 |
| HinfI | GANTC | 2 cut(s) | 768, 1102 |
| HpaII | CCGG | 1 cut(s) | 668 |
| HphI | GGTGA | 3 cut(s) | 25, 379, 562 |
| Hpy166II | GTNNAC | 5 cut(s) | 291, 342, 706, 781, 1063 |
| Hpy188I | TCNGA | 3 cut(s) | 514, 597, 1178 |
| Hpy188III | TCNNGA | 6 cut(s) | 211, 230, 967, 1087, 1426, 1454 |
| Hpy8I | GTNNAC | 5 cut(s) | 291, 342, 706, 781, 1063 |
| Hpy99I | CGWCG | 1 cut(s) | 1080 |
| HpyAV | CCTTC | 2 cut(s) | 740, 1369 |
| HpyCH4III | ACNGT | 5 cut(s) | 217, 260, 685, 719, 1042 |
| HpyCH4V | TGCA | 6 cut(s) | 248, 335, 521, 590, 907, 938 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 320, 416, 491, 1232, 1289, 1298, 1302 |
| HpyF3I | CTNAG | 3 cut(s) | 7, 761, 1455 |
| Hsp92I | GRCGYC | 1 cut(s) | 57 |
| Hsp92II | CATG | 8 cut(s) | 43, 376, 442, 946, 1239, 1333, 1384, 1429 |
| HspAI | GCGC | 1 cut(s) | 57 |
| KasI | GGCGCC | 1 cut(s) | 56 |
| Ksp22I | TGATCA | 2 cut(s) | 105, 597 |
| KspI | CCGCGG | 1 cut(s) | 775 |
| Kzo9I | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| LguI | GCTCTTC | 1 cut(s) | 1340 |
| LmnI | GCTCC | 2 cut(s) | 539, 958 |
| Lsp1109I | GCAGC | 2 cut(s) | 137, 434 |
| LweI | GCATC | 3 cut(s) | 344, 894, 979 |
| MaeI | CTAG | 4 cut(s) | 356, 1196, 1253, 1269 |
| MaeIII | GTNAC | 3 cut(s) | 175, 279, 367 |
| MalI | GATC | 5 cut(s) | 92, 107, 599, 725, 1265 |
| MbiI | CCGCTC | 1 cut(s) | 132 |
| MboI | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| MboII | GAAGA | 9 cut(s) | 44, 199, 288, 547, 755, 777, 1327, 1531, 1553 |
| MfeI | CAATTG | 1 cut(s) | 1558 |
| MhlI | GDGCHC | 1 cut(s) | 544 |
| MlsI | TGGCCA | 1 cut(s) | 1305 |
| MluNI | TGGCCA | 1 cut(s) | 1305 |
| Mly113I | GGCGCC | 1 cut(s) | 57 |
| MmeI | TCCRAC | 4 cut(s) | 139, 617, 720, 940 |
| Mox20I | TGGCCA | 1 cut(s) | 1305 |
| MroXI | GAANNNNTTC | 1 cut(s) | 203 |
| MscI | TGGCCA | 1 cut(s) | 1305 |
| MslI | CAYNNNNRTG | 1 cut(s) | 896 |
| Msp20I | TGGCCA | 1 cut(s) | 1305 |
| MspA1I | CMGCKG | 1 cut(s) | 774 |
| MspI | CCGG | 1 cut(s) | 668 |
| MspR9I | CCNGG | 2 cut(s) | 240, 491 |
| MunI | CAATTG | 1 cut(s) | 1558 |
| Mva1269I | GAATGC | 2 cut(s) | 579, 1086 |
| MvaI | CCWGG | 2 cut(s) | 240, 491 |
| MvnI | CGCG | 1 cut(s) | 774 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 320, 416, 491, 1232, 1289, 1298, 1302 |
| NarI | GGCGCC | 1 cut(s) | 57 |
| NdeII | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| NlaIII | CATG | 8 cut(s) | 43, 376, 442, 946, 1239, 1333, 1384, 1429 |
| NlaIV | GGNNCC | 4 cut(s) | 58, 666, 1248, 1418 |
| NmeAIII | GCCGAG | 1 cut(s) | 1277 |
| NmuCI | GTSAC | 1 cut(s) | 367 |
| NspI | RCATGY | 1 cut(s) | 1333 |
| PagI | TCATGA | 1 cut(s) | 1425 |
| PceI | AGGCCT | 2 cut(s) | 138, 880 |
| PciSI | GCTCTTC | 1 cut(s) | 1340 |
| PctI | GAATGC | 2 cut(s) | 579, 1086 |
| PdmI | GAANNNNTTC | 1 cut(s) | 203 |
| PfeI | GAWTC | 2 cut(s) | 768, 1102 |
| Pfl23II | CGTACG | 1 cut(s) | 824 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 973 |
| PkrI | GCNGC | 4 cut(s) | 127, 313, 421, 424 |
| PluTI | GGCGCC | 1 cut(s) | 60 |
| PpuMI | RGGWCCY | 2 cut(s) | 1247, 1417 |
| PshBI | ATTAAT | 1 cut(s) | 933 |
| Psp5II | RGGWCCY | 2 cut(s) | 1247, 1417 |
| Psp6I | CCWGG | 2 cut(s) | 238, 489 |
| PspEI | GGTNACC | 1 cut(s) | 367 |
| PspFI | CCCAGC | 3 cut(s) | 383, 659, 1097 |
| PspGI | CCWGG | 2 cut(s) | 238, 489 |
| PspLI | CGTACG | 1 cut(s) | 824 |
| PspN4I | GGNNCC | 4 cut(s) | 58, 666, 1248, 1418 |
| PspPI | GGNCC | 3 cut(s) | 1247, 1417, 1547 |
| PspPPI | RGGWCCY | 2 cut(s) | 1247, 1417 |
| RsaI | GTAC | 4 cut(s) | 305, 341, 826, 1514 |
| RsaNI | GTAC | 4 cut(s) | 304, 340, 825, 1513 |
| RseI | CAYNNNNRTG | 1 cut(s) | 896 |
| SacII | CCGCGG | 1 cut(s) | 775 |
| SapI | GCTCTTC | 1 cut(s) | 1340 |
| SatI | GCNGC | 4 cut(s) | 126, 312, 420, 423 |
| Sau3AI | GATC | 5 cut(s) | 90, 105, 597, 723, 1263 |
| Sau96I | GGNCC | 3 cut(s) | 1247, 1417, 1547 |
| ScrFI | CCNGG | 2 cut(s) | 240, 491 |
| SduI | GDGCHC | 1 cut(s) | 544 |
| SfaNI | GCATC | 3 cut(s) | 344, 894, 979 |
| SfoI | GGCGCC | 1 cut(s) | 58 |
| Sfr303I | CCGCGG | 1 cut(s) | 775 |
| SgrBI | CCGCGG | 1 cut(s) | 775 |
| SinI | GGWCC | 3 cut(s) | 1247, 1417, 1547 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 896 |
| SseBI | AGGCCT | 2 cut(s) | 138, 880 |
| SsiI | CCGC | 6 cut(s) | 44, 132, 312, 420, 772, 774 |
| SspDI | GGCGCC | 1 cut(s) | 56 |
| SspI | AATATT | 1 cut(s) | 1468 |
| SspMI | CTAG | 4 cut(s) | 356, 1196, 1253, 1269 |
| StuI | AGGCCT | 2 cut(s) | 138, 880 |
| StyD4I | CCNGG | 2 cut(s) | 238, 489 |
| TaaI | ACNGT | 5 cut(s) | 217, 260, 685, 719, 1042 |
| TaqI | TCGA | 3 cut(s) | 361, 726, 1078 |
| TauI | GCSGC | 2 cut(s) | 314, 422 |
| TfiI | GAWTC | 2 cut(s) | 768, 1102 |
| TscAI | CASTG | 1 cut(s) | 654 |
| TseFI | GTSAC | 1 cut(s) | 367 |
| TseI | GCWGC | 2 cut(s) | 125, 422 |
| Tsp45I | GTSAC | 1 cut(s) | 367 |
| TspDTI | ATGAA | 5 cut(s) | 17, 288, 443, 1397, 1499 |
| TspGWI | ACGGA | 1 cut(s) | 997 |
| TspRI | CASTG | 1 cut(s) | 654 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 973 |
| VpaK11BI | GGWCC | 3 cut(s) | 1247, 1417, 1547 |
| VspI | ATTAAT | 1 cut(s) | 933 |
| XapI | RAATTY | 5 cut(s) | 77, 162, 203, 531, 1191 |
| XceI | RCATGY | 1 cut(s) | 1333 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1385 |
| XmiI | GTMKAC | 1 cut(s) | 705 |
| XmnI | GAANNNNTTC | 1 cut(s) | 203 |
| XspI | CTAG | 4 cut(s) | 356, 1196, 1253, 1269 |