Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 859 |
| AccB1I | GGYRCC | 1 cut(s) | 1510 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 934 |
| AccI | GTMKAC | 1 cut(s) | 666 |
| AciI | CCGC | 3 cut(s) | 5, 132, 1039 |
| AclWI | GGATC | 6 cut(s) | 425, 679, 724, 737, 746, 1219 |
| AcsI | RAATTY | 3 cut(s) | 164, 492, 1152 |
| AcuI | CTGAAG | 1 cut(s) | 796 |
| AfaI | GTAC | 4 cut(s) | 266, 302, 423, 1380 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| AflII | CTTAAG | 1 cut(s) | 824 |
| AgsI | TTSAA | 2 cut(s) | 44, 1439 |
| AjnI | CCWGG | 4 cut(s) | 199, 450, 762, 1373 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1280, 1312 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 702, 734 |
| AluBI | AGCT | 6 cut(s) | 89, 386, 523, 968, 1031, 1058 |
| AluI | AGCT | 6 cut(s) | 89, 386, 523, 968, 1031, 1058 |
| Alw26I | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| AlwI | GGATC | 6 cut(s) | 425, 679, 724, 737, 746, 1219 |
| AoxI | GGCC | 1 cut(s) | 319 |
| ApeKI | GCWGC | 4 cut(s) | 86, 272, 380, 383 |
| ApoI | RAATTY | 3 cut(s) | 164, 492, 1152 |
| AseI | ATTAAT | 1 cut(s) | 894 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 167, 809, 1484 |
| AspS9I | GGNCC | 1 cut(s) | 1208 |
| AsuHPI | GGTGA | 1 cut(s) | 1111 |
| AvaII | GGWCC | 1 cut(s) | 1208 |
| BaeGI | GKGCMC | 1 cut(s) | 505 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 990, 1023 |
| BamHI | GGATCC | 1 cut(s) | 729 |
| BanI | GGYRCC | 1 cut(s) | 1510 |
| BbsI | GAAGAC | 1 cut(s) | 1396 |
| BbvI | GCAGC | 4 cut(s) | 98, 259, 367, 395 |
| BccI | CCATC | 2 cut(s) | 591, 1096 |
| BciT130I | CCWGG | 4 cut(s) | 201, 452, 764, 1375 |
| BclI | TGATCA | 2 cut(s) | 66, 849 |
| BcoDI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BfaI | CTAG | 5 cut(s) | 90, 317, 611, 1052, 1214 |
| BfmI | CTRYAG | 3 cut(s) | 381, 1174, 1260 |
| BfrI | CTTAAG | 1 cut(s) | 824 |
| BfuAI | ACCTGC | 1 cut(s) | 859 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1250 |
| BisI | GCNGC | 5 cut(s) | 87, 273, 381, 384, 1039 |
| BlsI | GCNGC | 5 cut(s) | 88, 274, 382, 385, 1040 |
| Bme1390I | CCNGG | 4 cut(s) | 201, 452, 764, 1375 |
| Bme18I | GGWCC | 1 cut(s) | 1208 |
| BmgT120I | GGNCC | 1 cut(s) | 1208 |
| BmiI | GGNNCC | 4 cut(s) | 731, 836, 1209, 1512 |
| BmrFI | CCNGG | 4 cut(s) | 201, 452, 764, 1375 |
| BmsI | GCATC | 6 cut(s) | 305, 390, 855, 940, 962, 1196 |
| BpiI | GAAGAC | 1 cut(s) | 1396 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1241, 1273 |
| BpmI | CTGGAG | 2 cut(s) | 785, 813 |
| BpuEI | CTTGAG | 1 cut(s) | 1268 |
| BsaI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsaJI | CCNNGG | 5 cut(s) | 474, 733, 933, 1135, 1374 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| Bse1I | ACTGG | 3 cut(s) | 892, 917, 1475 |
| BseBI | CCWGG | 4 cut(s) | 201, 452, 764, 1375 |
| BseDI | CCNNGG | 5 cut(s) | 474, 733, 933, 1135, 1374 |
| BseGI | GGATG | 3 cut(s) | 602, 1187, 1498 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| BseMII | CTCAG | 2 cut(s) | 1383, 1407 |
| BseNI | ACTGG | 3 cut(s) | 892, 917, 1475 |
| BseSI | GKGCMC | 1 cut(s) | 505 |
| BseXI | GCAGC | 4 cut(s) | 98, 259, 367, 395 |
| BseYI | CCCAGC | 1 cut(s) | 344 |
| BshFI | GGCC | 1 cut(s) | 321 |
| BshNI | GGYRCC | 1 cut(s) | 1510 |
| BsiSI | CCGG | 1 cut(s) | 1350 |
| BslFI | GGGAC | 3 cut(s) | 1119, 1221, 1415 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| BsmAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BsmFI | GGGAC | 3 cut(s) | 1119, 1221, 1415 |
| BsmI | GAATGC | 1 cut(s) | 540 |
| BsnI | GGCC | 1 cut(s) | 321 |
| Bso31I | GGTCTC | 2 cut(s) | 657, 1412 |
| Bsp1286I | GDGCHC | 1 cut(s) | 505 |
| Bsp143I | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| BspACI | CCGC | 3 cut(s) | 5, 132, 1039 |
| BspANI | GGCC | 1 cut(s) | 321 |
| BspCNI | CTCAG | 2 cut(s) | 1382, 1408 |
| BspHI | TCATGA | 1 cut(s) | 1386 |
| BspLI | GGNNCC | 4 cut(s) | 731, 836, 1209, 1512 |
| BspMAI | CTGCAG | 3 cut(s) | 385, 1178, 1264 |
| BspMI | ACCTGC | 1 cut(s) | 859 |
| BspPI | GGATC | 6 cut(s) | 425, 679, 724, 737, 746, 1219 |
| BspQI | GCTCTTC | 1 cut(s) | 1301 |
| BspT107I | GGYRCC | 1 cut(s) | 1510 |
| BspTI | CTTAAG | 1 cut(s) | 824 |
| BspTNI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsrI | ACTGG | 3 cut(s) | 892, 917, 1475 |
| BssECI | CCNNGG | 5 cut(s) | 474, 733, 933, 1135, 1374 |
| BssMI | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| BssT1I | CCWWGG | 1 cut(s) | 933 |
| Bst2UI | CCWGG | 4 cut(s) | 201, 452, 764, 1375 |
| Bst4CI | ACNGT | 5 cut(s) | 249, 370, 646, 1003, 1402 |
| Bst6I | CTCTTC | 4 cut(s) | 521, 1301, 1434, 1475 |
| BstAFI | CTTAAG | 1 cut(s) | 824 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 219, 1193 |
| BstC8I | GCNNGC | 4 cut(s) | 622, 1043, 1255, 1524 |
| BstDEI | CTNAG | 2 cut(s) | 1369, 1416 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 473 |
| BstF5I | GGATG | 3 cut(s) | 602, 1187, 1498 |
| BstKTI | GATC | 9 cut(s) | 54, 69, 420, 687, 732, 754, 852, 1173, 1227 |
| BstMAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BstMBI | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 219, 281, 452, 713, 1193, 1250, 1259 |
| BstNI | CCWGG | 4 cut(s) | 201, 452, 764, 1375 |
| BstNSI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| BstSCI | CCNGG | 4 cut(s) | 199, 450, 762, 1373 |
| BstSFI | CTRYAG | 3 cut(s) | 381, 1174, 1260 |
| BstSLI | GKGCMC | 1 cut(s) | 505 |
| BstV1I | GCAGC | 4 cut(s) | 98, 259, 367, 395 |
| BstV2I | GAAGAC | 1 cut(s) | 1396 |
| BstX2I | RGATCY | 1 cut(s) | 729 |
| BstYI | RGATCY | 1 cut(s) | 729 |
| BsuRI | GGCC | 1 cut(s) | 321 |
| BtgZI | GCGATG | 1 cut(s) | 413 |
| BtsCI | GGATG | 3 cut(s) | 602, 1187, 1498 |
| BtsI | GCAGTG | 1 cut(s) | 1101 |
| BtsIMutI | CAGTG | 3 cut(s) | 885, 1101, 1468 |
| BveI | ACCTGC | 1 cut(s) | 859 |
| Cac8I | GCNNGC | 4 cut(s) | 622, 1043, 1255, 1524 |
| CciI | TCATGA | 1 cut(s) | 1386 |
| Cfr13I | GGNCC | 1 cut(s) | 1208 |
| Csp6I | GTAC | 4 cut(s) | 265, 301, 422, 1379 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 768, 803 |
| CviQI | GTAC | 4 cut(s) | 265, 301, 422, 1379 |
| DdeI | CTNAG | 2 cut(s) | 1369, 1416 |
| DpnI | GATC | 9 cut(s) | 53, 68, 419, 686, 731, 753, 851, 1172, 1226 |
| DpnII | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| Eam1104I | CTCTTC | 4 cut(s) | 521, 1301, 1434, 1475 |
| EarI | CTCTTC | 4 cut(s) | 521, 1301, 1434, 1475 |
| Eco130I | CCWWGG | 1 cut(s) | 933 |
| Eco31I | GGTCTC | 2 cut(s) | 657, 1412 |
| Eco47I | GGWCC | 1 cut(s) | 1208 |
| Eco57I | CTGAAG | 1 cut(s) | 796 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 473 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1208 |
| EcoRI | GAATTC | 2 cut(s) | 492, 1152 |
| EcoRII | CCWGG | 4 cut(s) | 199, 450, 762, 1373 |
| EcoT14I | CCWWGG | 1 cut(s) | 933 |
| EcoT22I | ATGCAT | 1 cut(s) | 955 |
| ErhI | CCWWGG | 1 cut(s) | 933 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 152, 184, 705, 737, 794, 826 |
| FaqI | GGGAC | 3 cut(s) | 1119, 1221, 1415 |
| FbaI | TGATCA | 2 cut(s) | 66, 849 |
| FblI | GTMKAC | 1 cut(s) | 666 |
| Fnu4HI | GCNGC | 5 cut(s) | 87, 273, 381, 384, 1039 |
| FokI | GGATG | 3 cut(s) | 609, 1174, 1505 |
| Fsp4HI | GCNGC | 5 cut(s) | 87, 273, 381, 384, 1039 |
| FspBI | CTAG | 5 cut(s) | 90, 317, 611, 1052, 1214 |
| GluI | GCNGC | 5 cut(s) | 87, 273, 381, 384, 1039 |
| GsaI | CCCAGC | 1 cut(s) | 348 |
| GsuI | CTGGAG | 2 cut(s) | 785, 813 |
| HaeIII | GGCC | 1 cut(s) | 321 |
| HapII | CCGG | 1 cut(s) | 1350 |
| HincII | GTYRAC | 2 cut(s) | 24, 999 |
| HindII | GTYRAC | 2 cut(s) | 24, 999 |
| HinfI | GANTC | 5 cut(s) | 99, 457, 805, 981, 1485 |
| HpaII | CCGG | 1 cut(s) | 1350 |
| HphI | GGTGA | 1 cut(s) | 1111 |
| Hpy166II | GTNNAC | 5 cut(s) | 24, 252, 667, 999, 1024 |
| Hpy188I | TCNGA | 5 cut(s) | 186, 475, 734, 815, 1139 |
| Hpy188III | TCNNGA | 5 cut(s) | 755, 928, 1387, 1415, 1477 |
| Hpy8I | GTNNAC | 5 cut(s) | 24, 252, 667, 999, 1024 |
| HpyAV | CCTTC | 2 cut(s) | 820, 852 |
| HpyCH4III | ACNGT | 5 cut(s) | 249, 370, 646, 1003, 1402 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 219, 281, 452, 713, 1193, 1250, 1259 |
| HpyF3I | CTNAG | 2 cut(s) | 1369, 1416 |
| Ksp22I | TGATCA | 2 cut(s) | 66, 849 |
| Kzo9I | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| LguI | GCTCTTC | 1 cut(s) | 1301 |
| Lsp1109I | GCAGC | 4 cut(s) | 98, 259, 367, 395 |
| LweI | GCATC | 6 cut(s) | 305, 390, 855, 940, 962, 1196 |
| MaeI | CTAG | 5 cut(s) | 90, 317, 611, 1052, 1214 |
| MaeIII | GTNAC | 2 cut(s) | 110, 136 |
| MalI | GATC | 9 cut(s) | 53, 68, 419, 686, 731, 753, 851, 1172, 1226 |
| MboI | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| MflI | RGATCY | 1 cut(s) | 729 |
| MhlI | GDGCHC | 1 cut(s) | 505 |
| MluCI | AATT | 8 cut(s) | 164, 214, 492, 562, 881, 1152, 1164, 1410 |
| MlyI | GAGTC | 2 cut(s) | 93, 975 |
| MmeI | TCCRAC | 4 cut(s) | 418, 681, 712, 901 |
| Mph1103I | ATGCAT | 1 cut(s) | 955 |
| MroXI | GAANNNNTTC | 3 cut(s) | 167, 809, 1484 |
| MseI | TTAA | 9 cut(s) | 278, 375, 507, 723, 825, 894, 942, 1116, 1409 |
| MslI | CAYNNNNRTG | 1 cut(s) | 143 |
| MspA1I | CMGCKG | 1 cut(s) | 968 |
| MspCI | CTTAAG | 1 cut(s) | 824 |
| MspI | CCGG | 1 cut(s) | 1350 |
| MspR9I | CCNGG | 4 cut(s) | 201, 452, 764, 1375 |
| Mva1269I | GAATGC | 1 cut(s) | 540 |
| MvaI | CCWGG | 4 cut(s) | 201, 452, 764, 1375 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 219, 281, 452, 713, 1193, 1250, 1259 |
| NdeII | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| NlaIV | GGNNCC | 4 cut(s) | 731, 836, 1209, 1512 |
| NmuCI | GTSAC | 1 cut(s) | 136 |
| NsiI | ATGCAT | 1 cut(s) | 955 |
| NspI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| PaeI | GCATGC | 2 cut(s) | 1045, 1526 |
| PagI | TCATGA | 1 cut(s) | 1386 |
| PciSI | GCTCTTC | 1 cut(s) | 1301 |
| PctI | GAATGC | 1 cut(s) | 540 |
| PdmI | GAANNNNTTC | 3 cut(s) | 167, 809, 1484 |
| PfeI | GAWTC | 3 cut(s) | 457, 805, 1485 |
| PflFI | GACNNNGTC | 1 cut(s) | 746 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 934 |
| PfoI | TCCNGGA | 1 cut(s) | 199 |
| PkrI | GCNGC | 5 cut(s) | 88, 274, 382, 385, 1040 |
| PleI | GAGTC | 2 cut(s) | 93, 975 |
| PpsI | GAGTC | 2 cut(s) | 93, 975 |
| PpuMI | RGGWCCY | 1 cut(s) | 1208 |
| PshBI | ATTAAT | 1 cut(s) | 894 |
| Psp5II | RGGWCCY | 1 cut(s) | 1208 |
| Psp6I | CCWGG | 4 cut(s) | 199, 450, 762, 1373 |
| PspFI | CCCAGC | 1 cut(s) | 344 |
| PspGI | CCWGG | 4 cut(s) | 199, 450, 762, 1373 |
| PspN4I | GGNNCC | 4 cut(s) | 731, 836, 1209, 1512 |
| PspPI | GGNCC | 1 cut(s) | 1208 |
| PspPPI | RGGWCCY | 1 cut(s) | 1208 |
| PstI | CTGCAG | 3 cut(s) | 385, 1178, 1264 |
| PsuI | RGATCY | 1 cut(s) | 729 |
| PsyI | GACNNNGTC | 1 cut(s) | 746 |
| PvuII | CAGCTG | 1 cut(s) | 968 |
| RsaI | GTAC | 4 cut(s) | 266, 302, 423, 1380 |
| RsaNI | GTAC | 4 cut(s) | 265, 301, 422, 1379 |
| RseI | CAYNNNNRTG | 1 cut(s) | 143 |
| SapI | GCTCTTC | 1 cut(s) | 1301 |
| SaqAI | TTAA | 9 cut(s) | 278, 375, 507, 723, 825, 894, 942, 1116, 1409 |
| SatI | GCNGC | 5 cut(s) | 87, 273, 381, 384, 1039 |
| Sau3AI | GATC | 9 cut(s) | 51, 66, 417, 684, 729, 751, 849, 1170, 1224 |
| Sau96I | GGNCC | 1 cut(s) | 1208 |
| SchI | GAGTC | 2 cut(s) | 93, 975 |
| ScrFI | CCNGG | 4 cut(s) | 201, 452, 764, 1375 |
| SduI | GDGCHC | 1 cut(s) | 505 |
| SfaNI | GCATC | 6 cut(s) | 305, 390, 855, 940, 962, 1196 |
| SfcI | CTRYAG | 3 cut(s) | 381, 1174, 1260 |
| SinI | GGWCC | 1 cut(s) | 1208 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 143 |
| SmlI | CTYRAG | 2 cut(s) | 824, 1247 |
| SmoI | CTYRAG | 2 cut(s) | 824, 1247 |
| SphI | GCATGC | 2 cut(s) | 1045, 1526 |
| Sse9I | AATT | 8 cut(s) | 164, 214, 492, 562, 881, 1152, 1164, 1410 |
| SsiI | CCGC | 3 cut(s) | 5, 132, 1039 |
| SspMI | CTAG | 5 cut(s) | 90, 317, 611, 1052, 1214 |
| StyD4I | CCNGG | 4 cut(s) | 199, 450, 762, 1373 |
| StyI | CCWWGG | 1 cut(s) | 933 |
| TaaI | ACNGT | 5 cut(s) | 249, 370, 646, 1003, 1402 |
| TaqI | TCGA | 1 cut(s) | 750 |
| TaqII | GACCGA | 1 cut(s) | 1467 |
| TasI | AATT | 8 cut(s) | 164, 214, 492, 562, 881, 1152, 1164, 1410 |
| TauI | GCSGC | 1 cut(s) | 1041 |
| TfiI | GAWTC | 3 cut(s) | 457, 805, 1485 |
| Tru1I | TTAA | 9 cut(s) | 278, 375, 507, 723, 825, 894, 942, 1116, 1409 |
| Tru9I | TTAA | 9 cut(s) | 278, 375, 507, 723, 825, 894, 942, 1116, 1409 |
| TscAI | CASTG | 3 cut(s) | 892, 1101, 1475 |
| TseFI | GTSAC | 1 cut(s) | 136 |
| TseI | GCWGC | 4 cut(s) | 86, 272, 380, 383 |
| Tsp45I | GTSAC | 1 cut(s) | 136 |
| TspDTI | ATGAA | 4 cut(s) | 1358, 1460, 1477, 1509 |
| TspGWI | ACGGA | 2 cut(s) | 582, 603 |
| TspRI | CASTG | 3 cut(s) | 892, 1101, 1475 |
| Tth111I | GACNNNGTC | 1 cut(s) | 746 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 934 |
| Vha464I | CTTAAG | 1 cut(s) | 824 |
| VpaK11BI | GGWCC | 1 cut(s) | 1208 |
| VspI | ATTAAT | 1 cut(s) | 894 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 473 |
| XapI | RAATTY | 3 cut(s) | 164, 492, 1152 |
| XceI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 884 |
| XmiI | GTMKAC | 1 cut(s) | 666 |
| XmnI | GAANNNNTTC | 3 cut(s) | 167, 809, 1484 |
| XspI | CTAG | 5 cut(s) | 90, 317, 611, 1052, 1214 |
| Zsp2I | ATGCAT | 1 cut(s) | 955 |