Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 17 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 934 |
| AccBSI | CCGCTC | 1 cut(s) | 93 |
| AccI | GTMKAC | 1 cut(s) | 666 |
| AccII | CGCG | 1 cut(s) | 735 |
| AciI | CCGC | 6 cut(s) | 5, 93, 273, 381, 733, 735 |
| AclWI | GGATC | 1 cut(s) | 1219 |
| AcoI | YGGCCR | 1 cut(s) | 1264 |
| AcsI | RAATTY | 5 cut(s) | 38, 123, 164, 492, 1152 |
| AcyI | GRCGYC | 1 cut(s) | 18 |
| AfaI | GTAC | 4 cut(s) | 266, 302, 787, 1475 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 934 |
| AgsI | TTSAA | 7 cut(s) | 44, 160, 752, 1067, 1250, 1329, 1502 |
| AjnI | CCWGG | 2 cut(s) | 199, 450 |
| AluBI | AGCT | 8 cut(s) | 344, 371, 523, 620, 1031, 1058, 1146, 1373 |
| AluI | AGCT | 8 cut(s) | 344, 371, 523, 620, 1031, 1058, 1146, 1373 |
| Alw21I | GWGCWC | 1 cut(s) | 505 |
| Alw26I | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| AlwI | GGATC | 1 cut(s) | 1219 |
| AoxI | GGCC | 3 cut(s) | 97, 839, 1264 |
| ApeKI | GCWGC | 2 cut(s) | 86, 383 |
| ApoI | RAATTY | 5 cut(s) | 38, 123, 164, 492, 1152 |
| AseI | ATTAAT | 1 cut(s) | 894 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 164 |
| AspLEI | GCGC | 1 cut(s) | 20 |
| AspS9I | GGNCC | 3 cut(s) | 1208, 1378, 1508 |
| AsuHPI | GGTGA | 2 cut(s) | 340, 523 |
| AvaII | GGWCC | 3 cut(s) | 1208, 1378, 1508 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 990, 1023 |
| BalI | TGGCCA | 1 cut(s) | 1266 |
| BanI | GGYRCC | 1 cut(s) | 17 |
| Bbv12I | GWGCWC | 1 cut(s) | 505 |
| BbvI | GCAGC | 2 cut(s) | 98, 395 |
| BccI | CCATC | 3 cut(s) | 1409, 1487, 1499 |
| BceAI | ACGGC | 2 cut(s) | 804, 1079 |
| BciT130I | CCWGG | 2 cut(s) | 201, 452 |
| BclI | TGATCA | 2 cut(s) | 66, 558 |
| BcoDI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BfaI | CTAG | 5 cut(s) | 317, 848, 1157, 1214, 1230 |
| BfoI | RGCGCY | 1 cut(s) | 21 |
| BisI | GCNGC | 4 cut(s) | 87, 273, 381, 384 |
| BlsI | GCNGC | 4 cut(s) | 88, 274, 382, 385 |
| Bme1390I | CCNGG | 2 cut(s) | 201, 452 |
| Bme18I | GGWCC | 3 cut(s) | 1208, 1378, 1508 |
| BmgT120I | GGNCC | 3 cut(s) | 1208, 1378, 1508 |
| BmiI | GGNNCC | 4 cut(s) | 19, 627, 1209, 1379 |
| BmrFI | CCNGG | 2 cut(s) | 201, 452 |
| BmrI | ACTGGG | 1 cut(s) | 620 |
| BmsI | GCATC | 3 cut(s) | 305, 855, 940 |
| BmuI | ACTGGG | 1 cut(s) | 620 |
| BpmI | CTGGAG | 1 cut(s) | 900 |
| BsaHI | GRCGYC | 1 cut(s) | 18 |
| BsaI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsaJI | CCNNGG | 2 cut(s) | 733, 1135 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1080, 1110 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 934 |
| Bse1I | ACTGG | 3 cut(s) | 615, 917, 1481 |
| Bse3DI | GCAATG | 1 cut(s) | 393 |
| BseBI | CCWGG | 2 cut(s) | 201, 452 |
| BseDI | CCNNGG | 2 cut(s) | 733, 1135 |
| BseGI | GGATG | 2 cut(s) | 965, 1498 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 934 |
| BseMI | GCAATG | 1 cut(s) | 393 |
| BseMII | CTCAG | 1 cut(s) | 1407 |
| BseNI | ACTGG | 3 cut(s) | 615, 917, 1481 |
| BseXI | GCAGC | 2 cut(s) | 98, 395 |
| BseYI | CCCAGC | 3 cut(s) | 344, 620, 1058 |
| Bsh1236I | CGCG | 1 cut(s) | 735 |
| BshFI | GGCC | 3 cut(s) | 99, 841, 1266 |
| BshNI | GGYRCC | 1 cut(s) | 17 |
| BsiHKAI | GWGCWC | 1 cut(s) | 505 |
| BsiSI | CCGG | 1 cut(s) | 629 |
| BsiWI | CGTACG | 1 cut(s) | 785 |
| BslFI | GGGAC | 1 cut(s) | 1119 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 934 |
| BsmAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BsmFI | GGGAC | 1 cut(s) | 1119 |
| BsmI | GAATGC | 2 cut(s) | 540, 1047 |
| BsnI | GGCC | 3 cut(s) | 99, 841, 1266 |
| Bso31I | GGTCTC | 2 cut(s) | 657, 1412 |
| Bsp1286I | GDGCHC | 1 cut(s) | 505 |
| Bsp143I | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| BspACI | CCGC | 6 cut(s) | 5, 93, 273, 381, 733, 735 |
| BspANI | GGCC | 3 cut(s) | 99, 841, 1266 |
| BspCNI | CTCAG | 1 cut(s) | 1408 |
| BspFNI | CGCG | 1 cut(s) | 735 |
| BspHI | TCATGA | 1 cut(s) | 1386 |
| BspLI | GGNNCC | 4 cut(s) | 19, 627, 1209, 1379 |
| BspPI | GGATC | 1 cut(s) | 1219 |
| BspQI | GCTCTTC | 1 cut(s) | 1301 |
| BspT107I | GGYRCC | 1 cut(s) | 17 |
| BspTNI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsrBI | CCGCTC | 1 cut(s) | 93 |
| BsrDI | GCAATG | 1 cut(s) | 393 |
| BsrI | ACTGG | 3 cut(s) | 615, 917, 1481 |
| BssECI | CCNNGG | 2 cut(s) | 733, 1135 |
| BssMI | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| BssNI | GRCGYC | 1 cut(s) | 18 |
| Bst2UI | CCWGG | 2 cut(s) | 201, 452 |
| Bst4CI | ACNGT | 5 cut(s) | 178, 221, 646, 680, 1003 |
| Bst6I | CTCTTC | 3 cut(s) | 521, 1301, 1475 |
| BstACI | GRCGYC | 1 cut(s) | 18 |
| BstC8I | GCNNGC | 3 cut(s) | 91, 1255, 1264 |
| BstDEI | CTNAG | 2 cut(s) | 722, 1416 |
| BstDSI | CCRYGG | 1 cut(s) | 733 |
| BstEII | GGTNACC | 1 cut(s) | 328 |
| BstF5I | GGATG | 2 cut(s) | 965, 1498 |
| BstFNI | CGCG | 1 cut(s) | 735 |
| BstH2I | RGCGCY | 1 cut(s) | 21 |
| BstHHI | GCGC | 1 cut(s) | 20 |
| BstKTI | GATC | 5 cut(s) | 54, 69, 561, 687, 1227 |
| BstMAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BstMBI | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 281, 377, 452, 1193, 1250, 1259, 1263 |
| BstNI | CCWGG | 2 cut(s) | 201, 452 |
| BstNSI | RCATGY | 1 cut(s) | 1294 |
| BstPI | GGTNACC | 1 cut(s) | 328 |
| BstSCI | CCNGG | 2 cut(s) | 199, 450 |
| BstUI | CGCG | 1 cut(s) | 735 |
| BstV1I | GCAGC | 2 cut(s) | 98, 395 |
| BsuRI | GGCC | 3 cut(s) | 99, 841, 1266 |
| BtgI | CCRYGG | 1 cut(s) | 733 |
| BtsCI | GGATG | 2 cut(s) | 965, 1498 |
| BtsIMutI | CAGTG | 1 cut(s) | 608 |
| Cac8I | GCNNGC | 3 cut(s) | 91, 1255, 1264 |
| CciI | TCATGA | 1 cut(s) | 1386 |
| CfoI | GCGC | 1 cut(s) | 20 |
| Cfr13I | GGNCC | 3 cut(s) | 1208, 1378, 1508 |
| Cfr42I | CCGCGG | 1 cut(s) | 736 |
| Csp6I | GTAC | 4 cut(s) | 265, 301, 786, 1474 |
| CviAII | CATG | 7 cut(s) | 334, 400, 904, 1197, 1291, 1342, 1387 |
| CviQI | GTAC | 4 cut(s) | 265, 301, 786, 1474 |
| DdeI | CTNAG | 2 cut(s) | 722, 1416 |
| DinI | GGCGCC | 1 cut(s) | 19 |
| DpnI | GATC | 5 cut(s) | 53, 68, 560, 686, 1226 |
| DpnII | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| EaeI | YGGCCR | 1 cut(s) | 1264 |
| Eam1104I | CTCTTC | 3 cut(s) | 521, 1301, 1475 |
| EarI | CTCTTC | 3 cut(s) | 521, 1301, 1475 |
| Eco147I | AGGCCT | 2 cut(s) | 99, 841 |
| Eco31I | GGTCTC | 2 cut(s) | 657, 1412 |
| Eco47I | GGWCC | 3 cut(s) | 1208, 1378, 1508 |
| Eco91I | GGTNACC | 1 cut(s) | 328 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1208, 1378 |
| EcoO65I | GGTNACC | 1 cut(s) | 328 |
| EcoRI | GAATTC | 2 cut(s) | 492, 1152 |
| EcoRII | CCWGG | 2 cut(s) | 199, 450 |
| EgeI | GGCGCC | 1 cut(s) | 19 |
| EheI | GGCGCC | 1 cut(s) | 19 |
| FaeI | CATG | 7 cut(s) | 337, 403, 907, 1200, 1294, 1345, 1390 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1308, 1340 |
| FaqI | GGGAC | 1 cut(s) | 1119 |
| FatI | CATG | 7 cut(s) | 333, 399, 903, 1196, 1290, 1341, 1386 |
| FbaI | TGATCA | 2 cut(s) | 66, 558 |
| FblI | GTMKAC | 1 cut(s) | 666 |
| Fnu4HI | GCNGC | 4 cut(s) | 87, 273, 381, 384 |
| FokI | GGATG | 2 cut(s) | 952, 1505 |
| Fsp4HI | GCNGC | 4 cut(s) | 87, 273, 381, 384 |
| FspBI | CTAG | 5 cut(s) | 317, 848, 1157, 1214, 1230 |
| GlaI | GCGC | 1 cut(s) | 19 |
| GluI | GCNGC | 4 cut(s) | 87, 273, 381, 384 |
| GsaI | CCCAGC | 3 cut(s) | 348, 624, 1062 |
| GsuI | CTGGAG | 1 cut(s) | 900 |
| HaeII | RGCGCY | 1 cut(s) | 21 |
| HaeIII | GGCC | 3 cut(s) | 99, 841, 1266 |
| HapII | CCGG | 1 cut(s) | 629 |
| HhaI | GCGC | 1 cut(s) | 20 |
| Hin1I | GRCGYC | 1 cut(s) | 18 |
| Hin1II | CATG | 7 cut(s) | 337, 403, 907, 1200, 1294, 1345, 1390 |
| Hin6I | GCGC | 1 cut(s) | 18 |
| HinP1I | GCGC | 1 cut(s) | 18 |
| HincII | GTYRAC | 1 cut(s) | 742 |
| HindII | GTYRAC | 1 cut(s) | 742 |
| HinfI | GANTC | 3 cut(s) | 729, 981, 1063 |
| HpaII | CCGG | 1 cut(s) | 629 |
| HphI | GGTGA | 2 cut(s) | 340, 523 |
| Hpy166II | GTNNAC | 5 cut(s) | 252, 303, 667, 742, 1024 |
| Hpy188I | TCNGA | 3 cut(s) | 475, 558, 1139 |
| Hpy188III | TCNNGA | 6 cut(s) | 172, 191, 928, 1048, 1387, 1415 |
| Hpy8I | GTNNAC | 5 cut(s) | 252, 303, 667, 742, 1024 |
| Hpy99I | CGWCG | 1 cut(s) | 1041 |
| HpyAV | CCTTC | 2 cut(s) | 701, 1330 |
| HpyCH4III | ACNGT | 5 cut(s) | 178, 221, 646, 680, 1003 |
| HpyCH4V | TGCA | 6 cut(s) | 209, 296, 482, 551, 868, 899 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 281, 377, 452, 1193, 1250, 1259, 1263 |
| HpyF3I | CTNAG | 2 cut(s) | 722, 1416 |
| Hsp92I | GRCGYC | 1 cut(s) | 18 |
| Hsp92II | CATG | 7 cut(s) | 337, 403, 907, 1200, 1294, 1345, 1390 |
| HspAI | GCGC | 1 cut(s) | 18 |
| KasI | GGCGCC | 1 cut(s) | 17 |
| Ksp22I | TGATCA | 2 cut(s) | 66, 558 |
| KspI | CCGCGG | 1 cut(s) | 736 |
| Kzo9I | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| LguI | GCTCTTC | 1 cut(s) | 1301 |
| LmnI | GCTCC | 2 cut(s) | 500, 919 |
| Lsp1109I | GCAGC | 2 cut(s) | 98, 395 |
| LweI | GCATC | 3 cut(s) | 305, 855, 940 |
| MaeI | CTAG | 5 cut(s) | 317, 848, 1157, 1214, 1230 |
| MaeIII | GTNAC | 3 cut(s) | 136, 240, 328 |
| MalI | GATC | 5 cut(s) | 53, 68, 560, 686, 1226 |
| MbiI | CCGCTC | 1 cut(s) | 93 |
| MboI | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| MboII | GAAGA | 7 cut(s) | 160, 249, 508, 738, 1288, 1492, 1514 |
| MfeI | CAATTG | 1 cut(s) | 1519 |
| MhlI | GDGCHC | 1 cut(s) | 505 |
| MlsI | TGGCCA | 1 cut(s) | 1266 |
| MluNI | TGGCCA | 1 cut(s) | 1266 |
| Mly113I | GGCGCC | 1 cut(s) | 18 |
| MlyI | GAGTC | 1 cut(s) | 975 |
| MmeI | TCCRAC | 4 cut(s) | 100, 578, 681, 901 |
| Mox20I | TGGCCA | 1 cut(s) | 1266 |
| MroXI | GAANNNNTTC | 1 cut(s) | 164 |
| MscI | TGGCCA | 1 cut(s) | 1266 |
| MslI | CAYNNNNRTG | 1 cut(s) | 857 |
| Msp20I | TGGCCA | 1 cut(s) | 1266 |
| MspA1I | CMGCKG | 1 cut(s) | 735 |
| MspI | CCGG | 1 cut(s) | 629 |
| MspR9I | CCNGG | 2 cut(s) | 201, 452 |
| MunI | CAATTG | 1 cut(s) | 1519 |
| Mva1269I | GAATGC | 2 cut(s) | 540, 1047 |
| MvaI | CCWGG | 2 cut(s) | 201, 452 |
| MvnI | CGCG | 1 cut(s) | 735 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 281, 377, 452, 1193, 1250, 1259, 1263 |
| NarI | GGCGCC | 1 cut(s) | 18 |
| NdeII | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| NlaIII | CATG | 7 cut(s) | 337, 403, 907, 1200, 1294, 1345, 1390 |
| NlaIV | GGNNCC | 4 cut(s) | 19, 627, 1209, 1379 |
| NmeAIII | GCCGAG | 1 cut(s) | 1238 |
| NmuCI | GTSAC | 1 cut(s) | 328 |
| NspI | RCATGY | 1 cut(s) | 1294 |
| PagI | TCATGA | 1 cut(s) | 1386 |
| PceI | AGGCCT | 2 cut(s) | 99, 841 |
| PciSI | GCTCTTC | 1 cut(s) | 1301 |
| PctI | GAATGC | 2 cut(s) | 540, 1047 |
| PdmI | GAANNNNTTC | 1 cut(s) | 164 |
| PfeI | GAWTC | 2 cut(s) | 729, 1063 |
| Pfl23II | CGTACG | 1 cut(s) | 785 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 934 |
| PkrI | GCNGC | 4 cut(s) | 88, 274, 382, 385 |
| PleI | GAGTC | 1 cut(s) | 975 |
| PluTI | GGCGCC | 1 cut(s) | 21 |
| PpsI | GAGTC | 1 cut(s) | 975 |
| PpuMI | RGGWCCY | 2 cut(s) | 1208, 1378 |
| PshBI | ATTAAT | 1 cut(s) | 894 |
| Psp5II | RGGWCCY | 2 cut(s) | 1208, 1378 |
| Psp6I | CCWGG | 2 cut(s) | 199, 450 |
| PspEI | GGTNACC | 1 cut(s) | 328 |
| PspFI | CCCAGC | 3 cut(s) | 344, 620, 1058 |
| PspGI | CCWGG | 2 cut(s) | 199, 450 |
| PspLI | CGTACG | 1 cut(s) | 785 |
| PspN4I | GGNNCC | 4 cut(s) | 19, 627, 1209, 1379 |
| PspPI | GGNCC | 3 cut(s) | 1208, 1378, 1508 |
| PspPPI | RGGWCCY | 2 cut(s) | 1208, 1378 |
| RsaI | GTAC | 4 cut(s) | 266, 302, 787, 1475 |
| RsaNI | GTAC | 4 cut(s) | 265, 301, 786, 1474 |
| RseI | CAYNNNNRTG | 1 cut(s) | 857 |
| SacII | CCGCGG | 1 cut(s) | 736 |
| SapI | GCTCTTC | 1 cut(s) | 1301 |
| SatI | GCNGC | 4 cut(s) | 87, 273, 381, 384 |
| Sau3AI | GATC | 5 cut(s) | 51, 66, 558, 684, 1224 |
| Sau96I | GGNCC | 3 cut(s) | 1208, 1378, 1508 |
| SchI | GAGTC | 1 cut(s) | 975 |
| ScrFI | CCNGG | 2 cut(s) | 201, 452 |
| SduI | GDGCHC | 1 cut(s) | 505 |
| SfaNI | GCATC | 3 cut(s) | 305, 855, 940 |
| SfoI | GGCGCC | 1 cut(s) | 19 |
| Sfr303I | CCGCGG | 1 cut(s) | 736 |
| SgrBI | CCGCGG | 1 cut(s) | 736 |
| SinI | GGWCC | 3 cut(s) | 1208, 1378, 1508 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 857 |
| SseBI | AGGCCT | 2 cut(s) | 99, 841 |
| SsiI | CCGC | 6 cut(s) | 5, 93, 273, 381, 733, 735 |
| SspDI | GGCGCC | 1 cut(s) | 17 |
| SspI | AATATT | 2 cut(s) | 761, 1429 |
| SspMI | CTAG | 5 cut(s) | 317, 848, 1157, 1214, 1230 |
| StuI | AGGCCT | 2 cut(s) | 99, 841 |
| StyD4I | CCNGG | 2 cut(s) | 199, 450 |
| TaaI | ACNGT | 5 cut(s) | 178, 221, 646, 680, 1003 |
| TaqI | TCGA | 3 cut(s) | 322, 687, 1039 |
| TauI | GCSGC | 2 cut(s) | 275, 383 |
| TfiI | GAWTC | 2 cut(s) | 729, 1063 |
| TscAI | CASTG | 1 cut(s) | 615 |
| TseFI | GTSAC | 1 cut(s) | 328 |
| TseI | GCWGC | 2 cut(s) | 86, 383 |
| Tsp45I | GTSAC | 1 cut(s) | 328 |
| TspDTI | ATGAA | 4 cut(s) | 249, 404, 1358, 1460 |
| TspGWI | ACGGA | 1 cut(s) | 958 |
| TspRI | CASTG | 1 cut(s) | 615 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 934 |
| VpaK11BI | GGWCC | 3 cut(s) | 1208, 1378, 1508 |
| VspI | ATTAAT | 1 cut(s) | 894 |
| XapI | RAATTY | 5 cut(s) | 38, 123, 164, 492, 1152 |
| XceI | RCATGY | 1 cut(s) | 1294 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1346 |
| XmiI | GTMKAC | 1 cut(s) | 666 |
| XmnI | GAANNNNTTC | 1 cut(s) | 164 |
| XspI | CTAG | 5 cut(s) | 317, 848, 1157, 1214, 1230 |