pycom13g16350
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000367
Physical Location & Seq
Reverse (-)
12061136 .. 12066578
5443 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16350.1

Sequence Viewer

Length: 1587 bp
ATGGCCGGCGGAGGGTTCTCAGCGGGTCCTGGCGGGGGTGAGTTCGAGGCAAAGATCACGCCCATCGTCATCATCTCGTGTATAATGGCTGCTACTGGTGGACTCATGTTTGGTTACGATGTTGGCGTTTCAGGTGGTGTTACATCTATGTCCCCATTCTTGAAAAAGTTCTTCCCGGTTGTATACAGAAGGACACAGGAACAGGGGATCAACAGCAATTACTGCAAATACGACAACCAGGGGCTGCAGTTATTCACGTCGTCGCTGTACCTGGCCGGCTTAACCGCCACATTCGCCGCTTCCTACACCACCAGGAAGTTCGGGCGGAGGCCGTCTATGCTGATTGCTGGGATTTTCTTCATTTTCGGCACAATTCTCAATGCTGCAGCTCAAGACCTCGCCATGCTCATCATTGGCAGGATCTCACTTGGTTGTGGAGTTGGGTTCGCCAATCAGGCTGTACCATTGTTTCTTTCGGAGATTGCACCGACGAGAATCCGCGGAGGGCTAAACATACTGTTCCAGCTTAATGTCACACTTGGCATTCTATTTGCAAACCTCGTTAATTATGGCACCAACAAGATCACAGGAGGATGGGGTTGGAGGGTGTCATTGGGGCTGGCTGGTTTTCCGGCCGGTCTCCTAACCTTGGGAGCTCTCTTGGTGGTCGAAACTCCTAACAGTCTCGTAGAGCGTGGTCTCTTAGAAGAAGGAAAATCTGTACTTAAGAGGATCCGCGGCACTGAAAATGTTGAACCTGAGTTCTTGGAACTTGTCGAAGCTAGTCGCATAGCTAAAGAAGTGAAGCACCCCTTCAGAAATCTCTTAAAGCGCAGGAATCGTCCCCAACTAATCATTGCAGTAGCGTTACAGATCTTTCAACAATTTACTGGGATCAATGCCATAATGTTTTACGCGCCAGTCCTGTTCAACACATTAGGGTTTGGAAACGATGCTGCCCTCTACTCAGCAGTTATCACAGGAGCCGTCAATGTTCTCTCCACCACTGTATCCATCTACTCAGTTGACAAAGCAGGCCGCCGCTTGCTCTTACTGCAAGCCGGTGTTCAAATGTTCATCTCTCAAGTGGCAATAGCCATAATACTAGGGATCAAGGTTAATGACCACTCCGATGACCTCCACAAAGGCTTCGCGATCTTCGTGGTTGGTCTGATTTGCACTTATGTTGCTGCGTTCGCTTGGTCTTGGGGGCCTCTAGCTTGGTTGATACCTAGTGAGACATTCCCACTAGAGACCCGCTCAGCCGGGCAAAGTGTGACTGTCTGCATCAACTTGCTCTTCACTTTCGTTATAGCGCAGGCCTTCCTCTCGATGCTTTGCCACTTCAAGTATGGAATCTTCCTCTTCTTCTCCGGGTGGGTTTTGGTCATGTCGTTCTTCGTGTTGTTCCTGCTCCCGGAGACGAAGAACATCCCCATTGAAGAGATGACAGAGAGGGTGTGGAAGCAGCATTGGTTGTGGAAGAGGTTTATGGACGACGATGACTACCACATTGAAGGAAATCATGGAGATGGCTTAAAAAAAAATGGGCATGCTAATGGATTTGATGGTGTTTCTCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

529

Amino Acids

57.76

Weight (kDa)

9.0

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 27 - 485 1.8e-121 Sugar (and other) transporter
MFS_1 PF07690 81 - 430 3.3e-23 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26340
fragaria_vesca FvH4_2g38270 FvH4_4g15150 FvH4_4g15160 FvH4_4g15170 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15180 FvH4_4g15220 FvH4_4g15221
malus_domestica MD13G1189100.v1.1 MD13G1189500.v1.1 MD13G1189600.v1.1 MD13G1189800.v1.1 MD13G1190100.v1.1 MD13G1190200.v1.1 MD13G1190300.v1.1 MD16G1189900.v1.1 MD16G1190000.v1.1 MD16G1190100.v1.1 MD16G1190300.v1.1
prunus_persica Prupe.1G156300_v2.0.a1 Prupe.1G156300_v2.0.a1 Prupe.1G156400_v2.0.a1 Prupe.1G156500_v2.0.a1 Prupe.1G156600_v2.0.a1 Prupe.1G156700_v2.0.a1 Prupe.1G156800_v2.0.a1 Prupe.1G156900_v2.0.a1 Prupe.1G157000_v2.0.a1
pyrus_communis pycom13g16350 pycom13g16370 pycom13g16380 pycom16g15990 pycom16g16010 pycom16g16020
rosa_chinensis RchiOBHm_Chr4g0413631 RchiOBHm_Chr4g0416271 RchiOBHm_Chr4g0416631 RchiOBHm_Chr4g0416651 RchiOBHm_Chr4g0416671 RchiOBHm_Chr4g0416681 RchiOBHm_Chr4g0416691 RchiOBHm_Chr4g0416721
rosa_laevigata RLG00000007958 RLG00000007959 RLG00000007960 RLG00000007961 RLG00000007963 RLG00000007964 RLG00000008204
rosa_multiflora Rmu_ssc0000377.1_g000001 Rmu_ssc0000377.1_g000007 Rmu_ssc0000377.1_g000017 Rmu_ssc0000377.1_g000025 Rmu_ssc0000377.1_g000041 Rmu_ssc0000377.1_g000043
rosa_roxburghii Rroxscaffold_5G00357580 Rroxscaffold_5G00360530 Rroxscaffold_5G00360560 Rroxscaffold_5G00360580 Rroxscaffold_5G00360590
rosa_rugosa Rorug04G0121900 Rorug04G0143800 Rorug04G0143900 Rorug04G0144000 Rorug04G0144000 Rorug04G0144100
rosa_samantha Rh4AG181300 Rh4AG206000 Rh4AG206100 Rh4AG206200 Rh4AG206300 Rh4AG206500 Rh4AG206700 Rh4BG180600 Rh4BG203400 Rh4BG203500 Rh4BG203600 Rh4BG203700 Rh4BG203800 Rh4BG203900 Rh4BG204000 Rh4CG192500 Rh4CG216900 Rh4CG217200 Rh4CG217500 Rh4CG217700 Rh4CG217800 Rh4DG176700 Rh4DG202900 Rh4DG203000 Rh4DG203200 Rh4DG203300 Rh4DG203900
rosa_wichuraiana Rw4G015160 Rw4G017430 Rw4G017440 Rw4G017450 Rw4G017460 Rw4G017480 Rw4G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 572
AccBSI CCGCTC 1 cut(s) 1260
AccI GTMKAC 1 cut(s) 183
AccII CGCG 4 cut(s) 501, 738, 917, 1154
AclWI GGATC 6 cut(s) 215, 428, 727, 740, 902, 1118
AcoI YGGCCR 3 cut(s) 3, 273, 633
AcuI CTGAAG 1 cut(s) 799
AfaI GTAC 3 cut(s) 269, 462, 723
AfiI CCNNNNNNNGG 4 cut(s) 12, 35, 649, 1417
AflII CTTAAG 1 cut(s) 725
AgsI TTSAA 8 cut(s) 163, 755, 881, 931, 1070, 1348, 1442, 1517
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 4 cut(s) 28, 237, 270, 311
AluBI AGCT 6 cut(s) 389, 526, 656, 782, 794, 1220
AluI AGCT 6 cut(s) 389, 526, 656, 782, 794, 1220
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
AlwI GGATC 6 cut(s) 215, 428, 727, 740, 902, 1118
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 7 cut(s) 3, 273, 329, 633, 1036, 1211, 1320
ApeKI GCWGC 7 cut(s) 89, 244, 383, 386, 956, 1190, 1468
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 3 cut(s) 834, 919, 1318
AspS9I GGNCC 2 cut(s) 26, 1211
AsuC2I CCSGG 4 cut(s) 176, 1267, 1375, 1418
AsuHPI GGTGA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 26
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BamHI GGATCC 1 cut(s) 732
BanI GGYRCC 1 cut(s) 572
BanII GRGCYC 1 cut(s) 658
BarI GAAGNNNNNNTAC 2 cut(s) 1343, 1375
BauI CACGAG 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 7 cut(s) 76, 231, 370, 398, 943, 1177, 1480
BccI CCATC 5 cut(s) 71, 588, 1022, 1526, 1562
BceAI ACGGC 2 cut(s) 316, 971
BciT130I CCWGG 4 cut(s) 30, 239, 272, 313
BciVI GTATCC 1 cut(s) 1021
BcnI CCSGG 4 cut(s) 176, 1267, 1375, 1418
BcoDI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BfaI CTAG 5 cut(s) 783, 1106, 1217, 1233, 1250
BfmI CTRYAG 2 cut(s) 245, 384
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 1021
BglI GCCNNNNNGGC 1 cut(s) 455
BglII AGATCT 1 cut(s) 873
BlpI GCTNAGC 1 cut(s) 1261
Bme1390I CCNGG 8 cut(s) 30, 176, 239, 272, 313, 1267, 1375, 1418
Bme18I GGWCC 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 26, 1211
BmiI GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
BmrFI CCNGG 8 cut(s) 30, 176, 239, 272, 313, 1267, 1375, 1418
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 3 cut(s) 943, 1296, 1323
BmuI ACTGGG 1 cut(s) 900
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
Bpu1102I GCTNAGC 1 cut(s) 1261
BpuEI CTTGAG 2 cut(s) 375, 1068
BpuMI CCSGG 4 cut(s) 176, 1267, 1375, 1418
BsaI GGTCTC 3 cut(s) 644, 704, 1247
BsaJI CCNNGG 4 cut(s) 238, 499, 648, 736
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 35, 649, 1417
Bse118I RCCGGY 4 cut(s) 5, 275, 635, 1061
Bse1I ACTGG 3 cut(s) 100, 895, 920
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 4 cut(s) 30, 239, 272, 313
BseDI CCNNGG 4 cut(s) 238, 499, 648, 736
BseGI GGATG 2 cut(s) 599, 1431
BseLI CCNNNNNNNGG 4 cut(s) 12, 35, 649, 1417
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 5 cut(s) 33, 750, 981, 1035, 1275
BseNI ACTGG 3 cut(s) 100, 895, 920
BseX3I CGGCCG 1 cut(s) 633
BseXI GCAGC 7 cut(s) 76, 231, 370, 398, 943, 1177, 1480
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 4 cut(s) 501, 738, 917, 1154
Bsh1285I CGRYCG 1 cut(s) 636
BshFI GGCC 7 cut(s) 5, 275, 331, 635, 1038, 1213, 1322
BshNI GGYRCC 1 cut(s) 572
BsiEI CGRYCG 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 658
BsiSI CCGG 9 cut(s) 6, 176, 276, 632, 636, 1062, 1266, 1374, 1418
BslFI GGGAC 2 cut(s) 136, 828
BslI CCNNNNNNNGG 4 cut(s) 12, 35, 649, 1417
BsmAI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BsmBI CGTCTC 1 cut(s) 1415
BsmFI GGGAC 2 cut(s) 136, 828
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 7 cut(s) 5, 275, 331, 635, 1038, 1213, 1322
Bso31I GGTCTC 3 cut(s) 644, 704, 1247
Bsp1286I GDGCHC 1 cut(s) 658
Bsp143I GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
Bsp1720I GCTNAGC 1 cut(s) 1261
Bsp68I TCGCGA 1 cut(s) 1154
BspANI GGCC 7 cut(s) 5, 275, 331, 635, 1038, 1213, 1322
BspCNI CTCAG 5 cut(s) 32, 751, 980, 1034, 1274
BspFNI CGCG 4 cut(s) 501, 738, 917, 1154
BspLI GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
BspMAI CTGCAG 2 cut(s) 249, 388
BspPI GGATC 6 cut(s) 215, 428, 727, 740, 902, 1118
BspQI GCTCTTC 1 cut(s) 1304
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 725
BspTNI GGTCTC 3 cut(s) 644, 704, 1247
BsrBI CCGCTC 1 cut(s) 1260
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 4 cut(s) 5, 275, 635, 1061
BsrI ACTGG 3 cut(s) 100, 895, 920
BssAI RCCGGY 4 cut(s) 5, 275, 635, 1061
BssECI CCNNGG 4 cut(s) 238, 499, 648, 736
BssMI GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
BssNAI GTATAC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 648
Bst1107I GTATAC 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 76
Bst2UI CCWGG 4 cut(s) 30, 239, 272, 313
Bst4CI ACNGT 4 cut(s) 519, 683, 1009, 1282
Bst6I CTCTTC 4 cut(s) 1304, 1370, 1437, 1478
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 8 cut(s) 7, 277, 621, 1036, 1046, 1059, 1320, 1554
BstDEI CTNAG 7 cut(s) 19, 703, 759, 967, 1021, 1261, 1578
BstDSI CCRYGG 2 cut(s) 499, 736
BstF5I GGATG 2 cut(s) 599, 1431
BstFNI CGCG 4 cut(s) 501, 738, 917, 1154
BstHHI GCGC 3 cut(s) 834, 919, 1318
BstKTI GATC 9 cut(s) 57, 210, 423, 585, 735, 876, 897, 1113, 1158
BstMAI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BstMBI GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 1054, 1196
BstNI CCWGG 4 cut(s) 30, 239, 272, 313
BstNSI RCATGY 1 cut(s) 1556
BstSCI CCNGG 8 cut(s) 28, 174, 237, 270, 311, 1265, 1373, 1416
BstSFI CTRYAG 2 cut(s) 245, 384
BstUI CGCG 4 cut(s) 501, 738, 917, 1154
BstV1I GCAGC 7 cut(s) 76, 231, 370, 398, 943, 1177, 1480
BstX2I RGATCY 3 cut(s) 420, 732, 873
BstYI RGATCY 3 cut(s) 420, 732, 873
BstZ17I GTATAC 1 cut(s) 184
BstZI CGGCCG 1 cut(s) 633
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 7 cut(s) 5, 275, 331, 635, 1038, 1213, 1322
BtgI CCRYGG 2 cut(s) 499, 736
BtrI CACGTC 1 cut(s) 258
BtsCI GGATG 2 cut(s) 599, 1431
BtsIMutI CAGTG 2 cut(s) 741, 1005
BtuMI TCGCGA 1 cut(s) 1154
Cac8I GCNNGC 8 cut(s) 7, 277, 621, 1036, 1046, 1059, 1320, 1554
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 3 cut(s) 834, 919, 1318
Cfr10I RCCGGY 4 cut(s) 5, 275, 635, 1061
Cfr13I GGNCC 2 cut(s) 26, 1211
Cfr42I CCGCGG 2 cut(s) 502, 739
Csp6I GTAC 3 cut(s) 268, 461, 722
CviAII CATG 5 cut(s) 106, 403, 1390, 1526, 1553
CviQI GTAC 3 cut(s) 268, 461, 722
DdeI CTNAG 7 cut(s) 19, 703, 759, 967, 1021, 1261, 1578
DpnI GATC 9 cut(s) 56, 209, 422, 584, 734, 875, 896, 1112, 1157
DpnII GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
EaeI YGGCCR 3 cut(s) 3, 273, 633
EagI CGGCCG 1 cut(s) 633
Eam1104I CTCTTC 4 cut(s) 1304, 1370, 1437, 1478
EarI CTCTTC 4 cut(s) 1304, 1370, 1437, 1478
EciI GGCGGA 2 cut(s) 24, 340
Ecl136II GAGCTC 1 cut(s) 656
EclXI CGGCCG 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 648
Eco147I AGGCCT 1 cut(s) 1322
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 3 cut(s) 644, 704, 1247
Eco47I GGWCC 1 cut(s) 26
Eco52I CGGCCG 1 cut(s) 633
Eco53kI GAGCTC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 799
EcoICRI GAGCTC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 26, 1211
EcoRII CCWGG 4 cut(s) 28, 237, 270, 311
EcoT14I CCWWGG 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 648
Esp3I CGTCTC 1 cut(s) 1415
FaeI CATG 5 cut(s) 109, 406, 1393, 1529, 1556
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FaqI GGGAC 2 cut(s) 136, 828
FatI CATG 5 cut(s) 105, 402, 1389, 1525, 1552
FauI CCCGC 3 cut(s) 16, 26, 1265
FblI GTMKAC 1 cut(s) 183
FokI GGATG 2 cut(s) 606, 1418
FriOI GRGCYC 1 cut(s) 658
FspBI CTAG 5 cut(s) 783, 1106, 1217, 1233, 1250
GlaI GCGC 3 cut(s) 833, 918, 1317
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 7 cut(s) 5, 275, 331, 635, 1038, 1213, 1322
HapII CCGG 9 cut(s) 6, 176, 276, 632, 636, 1062, 1266, 1374, 1418
HhaI GCGC 3 cut(s) 834, 919, 1318
Hin1II CATG 5 cut(s) 109, 406, 1393, 1529, 1556
Hin6I GCGC 3 cut(s) 832, 917, 1316
HinP1I GCGC 3 cut(s) 832, 917, 1316
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 4 cut(s) 102, 495, 838, 1356
HpaII CCGG 9 cut(s) 6, 176, 276, 632, 636, 1062, 1266, 1374, 1418
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 3 cut(s) 101, 184, 1027
Hpy188I TCNGA 4 cut(s) 478, 818, 1132, 1173
Hpy188III TCNNGA 4 cut(s) 160, 392, 1153, 1330
Hpy8I GTNNAC 3 cut(s) 101, 184, 1027
Hpy99I CGWCG 4 cut(s) 262, 265, 493, 1502
HpyAV CCTTC 5 cut(s) 183, 704, 823, 1333, 1511
HpyCH4III ACNGT 4 cut(s) 519, 683, 1009, 1282
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 9 cut(s) 225, 247, 386, 485, 554, 860, 1057, 1179, 1287
HpyF10VI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 1054, 1196
HpyF3I CTNAG 7 cut(s) 19, 703, 759, 967, 1021, 1261, 1578
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 5 cut(s) 109, 406, 1393, 1529, 1556
HspAI GCGC 3 cut(s) 832, 917, 1316
KroI GCCGGC 2 cut(s) 5, 275
KroNI GCCGGC 2 cut(s) 7, 277
KspI CCGCGG 2 cut(s) 502, 739
Kzo9I GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
LguI GCTCTTC 1 cut(s) 1304
LmnI GCTCC 3 cut(s) 653, 983, 1419
Lsp1109I GCAGC 7 cut(s) 76, 231, 370, 398, 943, 1177, 1480
LweI GCATC 3 cut(s) 943, 1296, 1323
MaeI CTAG 5 cut(s) 783, 1106, 1217, 1233, 1250
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 5 cut(s) 113, 139, 532, 867, 1276
MalI GATC 9 cut(s) 56, 209, 422, 584, 734, 875, 896, 1112, 1157
MbiI CCGCTC 1 cut(s) 1260
MboI GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
MflI RGATCY 3 cut(s) 420, 732, 873
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 4 cut(s) 217, 372, 565, 884
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 581
MroNI GCCGGC 2 cut(s) 5, 275
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 7 cut(s) 281, 528, 564, 726, 827, 1119, 1538
MslI CAYNNNNRTG 3 cut(s) 1131, 1530, 1557
MspA1I CMGCKG 3 cut(s) 23, 501, 738
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 9 cut(s) 6, 176, 276, 632, 636, 1062, 1266, 1374, 1418
MspR9I CCNGG 8 cut(s) 30, 176, 239, 272, 313, 1267, 1375, 1418
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 4 cut(s) 30, 239, 272, 313
MvnI CGCG 4 cut(s) 501, 738, 917, 1154
MwoI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 1054, 1196
NaeI GCCGGC 2 cut(s) 7, 277
NciI CCSGG 4 cut(s) 176, 1267, 1375, 1418
NdeII GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
NgoMIV GCCGGC 2 cut(s) 5, 275
NlaIII CATG 5 cut(s) 109, 406, 1393, 1529, 1556
NlaIV GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
NmuCI GTSAC 2 cut(s) 532, 1276
NruI TCGCGA 1 cut(s) 1154
NspI RCATGY 1 cut(s) 1556
PaeI GCATGC 1 cut(s) 1556
PceI AGGCCT 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 1304
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 1158
PctI GAATGC 1 cut(s) 543
PdiI GCCGGC 2 cut(s) 7, 277
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 3 cut(s) 495, 838, 1356
PfoI TCCNGGA 1 cut(s) 1416
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 4 cut(s) 28, 237, 270, 311
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 4 cut(s) 28, 237, 270, 311
PspN4I GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
PspPI GGNCC 2 cut(s) 26, 1211
PspPPI RGGWCCY 1 cut(s) 26
PstI CTGCAG 2 cut(s) 249, 388
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 3 cut(s) 420, 732, 873
RruI TCGCGA 1 cut(s) 1154
RsaI GTAC 3 cut(s) 269, 462, 723
RsaNI GTAC 3 cut(s) 268, 461, 722
RseI CAYNNNNRTG 3 cut(s) 1131, 1530, 1557
SacI GAGCTC 1 cut(s) 658
SacII CCGCGG 2 cut(s) 502, 739
SapI GCTCTTC 1 cut(s) 1304
SaqAI TTAA 7 cut(s) 281, 528, 564, 726, 827, 1119, 1538
Sau3AI GATC 9 cut(s) 54, 207, 420, 582, 732, 873, 894, 1110, 1155
Sau96I GGNCC 2 cut(s) 26, 1211
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 8 cut(s) 30, 176, 239, 272, 313, 1267, 1375, 1418
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 3 cut(s) 943, 1296, 1323
SfcI CTRYAG 2 cut(s) 245, 384
Sfr303I CCGCGG 2 cut(s) 502, 739
SgrBI CCGCGG 2 cut(s) 502, 739
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 3 cut(s) 1131, 1530, 1557
SmlI CTYRAG 3 cut(s) 390, 725, 1083
SmoI CTYRAG 3 cut(s) 390, 725, 1083
SphI GCATGC 1 cut(s) 1556
Sse9I AATT 4 cut(s) 217, 372, 565, 884
SseBI AGGCCT 1 cut(s) 1322
SspMI CTAG 5 cut(s) 783, 1106, 1217, 1233, 1250
SstI GAGCTC 1 cut(s) 658
StuI AGGCCT 1 cut(s) 1322
StyD4I CCNGG 8 cut(s) 28, 174, 237, 270, 311, 1265, 1373, 1416
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 4 cut(s) 519, 683, 1009, 1282
TaiI ACGT 1 cut(s) 260
TaqI TCGA 4 cut(s) 45, 669, 777, 1331
TasI AATT 4 cut(s) 217, 372, 565, 884
TatI WGTACW 1 cut(s) 721
TauI GCSGC 4 cut(s) 299, 741, 1041, 1044
TfiI GAWTC 3 cut(s) 495, 838, 1356
Tru1I TTAA 7 cut(s) 281, 528, 564, 726, 827, 1119, 1538
Tru9I TTAA 7 cut(s) 281, 528, 564, 726, 827, 1119, 1538
TscAI CASTG 2 cut(s) 748, 1012
TseFI GTSAC 2 cut(s) 532, 1276
TseI GCWGC 7 cut(s) 89, 244, 383, 386, 956, 1190, 1468
Tsp45I GTSAC 2 cut(s) 532, 1276
TspDTI ATGAA 2 cut(s) 349, 1066
TspRI CASTG 2 cut(s) 748, 1012
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 26
XceI RCATGY 1 cut(s) 1556
XcmI CCANNNNNNNNNTGG 1 cut(s) 1349
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 5 cut(s) 783, 1106, 1217, 1233, 1250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.