Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 859 |
| AccB1I | GGYRCC | 1 cut(s) | 950 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 934 |
| AccI | GTMKAC | 2 cut(s) | 180, 666 |
| AciI | CCGC | 4 cut(s) | 5, 630, 794, 1039 |
| AclWI | GGATC | 9 cut(s) | 425, 679, 724, 737, 746, 759, 800, 813, 1219 |
| AcsI | RAATTY | 4 cut(s) | 38, 164, 492, 1152 |
| AcuI | CTGAAG | 2 cut(s) | 796, 809 |
| AcyI | GRCGYC | 2 cut(s) | 296, 951 |
| AfaI | GTAC | 2 cut(s) | 266, 302 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| AflIII | ACRYGT | 1 cut(s) | 1290 |
| AgsI | TTSAA | 3 cut(s) | 160, 1345, 1439 |
| AjnI | CCWGG | 4 cut(s) | 199, 450, 1373, 1515 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1280, 1312 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 702, 734 |
| AluBI | AGCT | 5 cut(s) | 89, 328, 386, 523, 968 |
| AluI | AGCT | 5 cut(s) | 89, 328, 386, 523, 968 |
| Alw26I | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| AlwI | GGATC | 9 cut(s) | 425, 679, 724, 737, 746, 759, 800, 813, 1219 |
| Ama87I | CYCGRG | 1 cut(s) | 206 |
| AoxI | GGCC | 3 cut(s) | 319, 777, 839 |
| ApeKI | GCWGC | 5 cut(s) | 86, 272, 380, 383, 791 |
| ApoI | RAATTY | 4 cut(s) | 38, 164, 492, 1152 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 164, 167, 1484 |
| AspLEI | GCGC | 1 cut(s) | 953 |
| AspS9I | GGNCC | 2 cut(s) | 1208, 1378 |
| AsuC2I | CCSGG | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 1 cut(s) | 1174 |
| AsuII | TTCGAA | 1 cut(s) | 42 |
| AvaI | CYCGRG | 1 cut(s) | 206 |
| AvaII | GGWCC | 2 cut(s) | 1208, 1378 |
| BaeGI | GKGCMC | 1 cut(s) | 505 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 990, 1023 |
| BamHI | GGATCC | 3 cut(s) | 729, 751, 805 |
| BanI | GGYRCC | 1 cut(s) | 950 |
| BbsI | GAAGAC | 1 cut(s) | 194 |
| BbvI | GCAGC | 5 cut(s) | 98, 259, 367, 395, 778 |
| BccI | CCATC | 3 cut(s) | 591, 1096, 1498 |
| BciT130I | CCWGG | 4 cut(s) | 201, 452, 1375, 1517 |
| BclI | TGATCA | 1 cut(s) | 849 |
| BcnI | CCSGG | 1 cut(s) | 173 |
| BcoDI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BfaI | CTAG | 6 cut(s) | 90, 317, 611, 1052, 1214, 1247 |
| BfmI | CTRYAG | 3 cut(s) | 381, 645, 1174 |
| BfoI | RGCGCY | 1 cut(s) | 954 |
| BfuAI | ACCTGC | 1 cut(s) | 859 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 452 |
| BisI | GCNGC | 6 cut(s) | 87, 273, 381, 384, 792, 1039 |
| BlsI | GCNGC | 6 cut(s) | 88, 274, 382, 385, 793, 1040 |
| Bme1390I | CCNGG | 5 cut(s) | 173, 201, 452, 1375, 1517 |
| Bme18I | GGWCC | 2 cut(s) | 1208, 1378 |
| BmeT110I | CYCGRG | 1 cut(s) | 206 |
| BmgT120I | GGNCC | 2 cut(s) | 1208, 1378 |
| BmiI | GGNNCC | 6 cut(s) | 731, 753, 807, 952, 1209, 1379 |
| BmrFI | CCNGG | 5 cut(s) | 173, 201, 452, 1375, 1517 |
| BmsI | GCATC | 2 cut(s) | 390, 855 |
| BpiI | GAAGAC | 1 cut(s) | 194 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1241, 1273 |
| BpmI | CTGGAG | 1 cut(s) | 900 |
| Bpu14I | TTCGAA | 1 cut(s) | 42 |
| BpuMI | CCSGG | 1 cut(s) | 173 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1169 |
| BsaHI | GRCGYC | 2 cut(s) | 296, 951 |
| BsaI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsaJI | CCNNGG | 6 cut(s) | 733, 933, 1135, 1374, 1515, 1516 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| Bse118I | RCCGGY | 2 cut(s) | 1031, 1058 |
| Bse1I | ACTGG | 4 cut(s) | 780, 892, 917, 1475 |
| Bse3DI | GCAATG | 1 cut(s) | 852 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1169 |
| BseBI | CCWGG | 4 cut(s) | 201, 452, 1375, 1517 |
| BseDI | CCNNGG | 6 cut(s) | 733, 933, 1135, 1374, 1515, 1516 |
| BseGI | GGATG | 2 cut(s) | 602, 1187 |
| BseJI | GATNNNNATC | 1 cut(s) | 1169 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| BseMI | GCAATG | 1 cut(s) | 852 |
| BseMII | CTCAG | 2 cut(s) | 1383, 1407 |
| BseNI | ACTGG | 4 cut(s) | 780, 892, 917, 1475 |
| BseRI | GAGGAG | 1 cut(s) | 1477 |
| BseSI | GKGCMC | 1 cut(s) | 505 |
| BseXI | GCAGC | 5 cut(s) | 98, 259, 367, 395, 778 |
| BseYI | CCCAGC | 2 cut(s) | 344, 1262 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1036 |
| BshFI | GGCC | 3 cut(s) | 321, 779, 841 |
| BshNI | GGYRCC | 1 cut(s) | 950 |
| BsiEI | CGRYCG | 1 cut(s) | 1036 |
| BsiHKCI | CYCGRG | 1 cut(s) | 206 |
| BsiSI | CCGG | 4 cut(s) | 173, 1032, 1059, 1350 |
| BslFI | GGGAC | 2 cut(s) | 1119, 1221 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 475, 934, 1197 |
| BsmAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BsmFI | GGGAC | 2 cut(s) | 1119, 1221 |
| BsmI | GAATGC | 2 cut(s) | 540, 624 |
| BsnI | GGCC | 3 cut(s) | 321, 779, 841 |
| Bso31I | GGTCTC | 2 cut(s) | 657, 1412 |
| BsoBI | CYCGRG | 1 cut(s) | 206 |
| Bsp119I | TTCGAA | 1 cut(s) | 42 |
| Bsp1286I | GDGCHC | 1 cut(s) | 505 |
| BspACI | CCGC | 4 cut(s) | 5, 630, 794, 1039 |
| BspANI | GGCC | 3 cut(s) | 321, 779, 841 |
| BspCNI | CTCAG | 2 cut(s) | 1382, 1408 |
| BspHI | TCATGA | 1 cut(s) | 1386 |
| BspLI | GGNNCC | 6 cut(s) | 731, 753, 807, 952, 1209, 1379 |
| BspMAI | CTGCAG | 1 cut(s) | 385 |
| BspMI | ACCTGC | 1 cut(s) | 859 |
| BspPI | GGATC | 9 cut(s) | 425, 679, 724, 737, 746, 759, 800, 813, 1219 |
| BspT104I | TTCGAA | 1 cut(s) | 42 |
| BspT107I | GGYRCC | 1 cut(s) | 950 |
| BspTNI | GGTCTC | 2 cut(s) | 657, 1412 |
| BsrDI | GCAATG | 1 cut(s) | 852 |
| BsrFI | RCCGGY | 2 cut(s) | 1031, 1058 |
| BsrI | ACTGG | 4 cut(s) | 780, 892, 917, 1475 |
| BssAI | RCCGGY | 2 cut(s) | 1031, 1058 |
| BssECI | CCNNGG | 6 cut(s) | 733, 933, 1135, 1374, 1515, 1516 |
| BssNAI | GTATAC | 1 cut(s) | 181 |
| BssNI | GRCGYC | 2 cut(s) | 296, 951 |
| BssT1I | CCWWGG | 1 cut(s) | 933 |
| Bst1107I | GTATAC | 1 cut(s) | 181 |
| Bst2UI | CCWGG | 4 cut(s) | 201, 452, 1375, 1517 |
| Bst4CI | ACNGT | 4 cut(s) | 249, 370, 646, 1003 |
| Bst6I | CTCTTC | 3 cut(s) | 521, 1434, 1475 |
| BstACI | GRCGYC | 2 cut(s) | 296, 951 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 219 |
| BstBI | TTCGAA | 1 cut(s) | 42 |
| BstC8I | GCNNGC | 4 cut(s) | 622, 1043, 1255, 1524 |
| BstDEI | CTNAG | 2 cut(s) | 1369, 1416 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 473 |
| BstF5I | GGATG | 2 cut(s) | 602, 1187 |
| BstH2I | RGCGCY | 1 cut(s) | 954 |
| BstHHI | GCGC | 1 cut(s) | 953 |
| BstMAI | GTCTC | 3 cut(s) | 657, 1229, 1412 |
| BstMCI | CGRYCG | 1 cut(s) | 1036 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 219, 281, 334, 452, 1259 |
| BstNI | CCWGG | 4 cut(s) | 201, 452, 1375, 1517 |
| BstNSI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| BstSCI | CCNGG | 5 cut(s) | 171, 199, 450, 1373, 1515 |
| BstSFI | CTRYAG | 3 cut(s) | 381, 645, 1174 |
| BstSLI | GKGCMC | 1 cut(s) | 505 |
| BstV1I | GCAGC | 5 cut(s) | 98, 259, 367, 395, 778 |
| BstV2I | GAAGAC | 1 cut(s) | 194 |
| BstX2I | RGATCY | 4 cut(s) | 417, 729, 751, 805 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 787 |
| BstYI | RGATCY | 4 cut(s) | 417, 729, 751, 805 |
| BstZ17I | GTATAC | 1 cut(s) | 181 |
| BsuRI | GGCC | 3 cut(s) | 321, 779, 841 |
| BtgZI | GCGATG | 1 cut(s) | 413 |
| BtsCI | GGATG | 2 cut(s) | 602, 1187 |
| BtsIMutI | CAGTG | 2 cut(s) | 885, 1468 |
| BveI | ACCTGC | 1 cut(s) | 859 |
| Cac8I | GCNNGC | 4 cut(s) | 622, 1043, 1255, 1524 |
| CciI | TCATGA | 1 cut(s) | 1386 |
| CfoI | GCGC | 1 cut(s) | 953 |
| Cfr10I | RCCGGY | 2 cut(s) | 1031, 1058 |
| Cfr13I | GGNCC | 2 cut(s) | 1208, 1378 |
| CseI | GACGC | 3 cut(s) | 285, 1399, 1517 |
| Csp6I | GTAC | 2 cut(s) | 265, 301 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 273, 308 |
| CviAII | CATG | 9 cut(s) | 103, 334, 904, 1042, 1142, 1197, 1291, 1387, 1523 |
| CviQI | GTAC | 2 cut(s) | 265, 301 |
| DdeI | CTNAG | 2 cut(s) | 1369, 1416 |
| DinI | GGCGCC | 1 cut(s) | 952 |
| Eam1104I | CTCTTC | 3 cut(s) | 521, 1434, 1475 |
| EarI | CTCTTC | 3 cut(s) | 521, 1434, 1475 |
| Eco130I | CCWWGG | 1 cut(s) | 933 |
| Eco147I | AGGCCT | 1 cut(s) | 841 |
| Eco31I | GGTCTC | 2 cut(s) | 657, 1412 |
| Eco47I | GGWCC | 2 cut(s) | 1208, 1378 |
| Eco57I | CTGAAG | 2 cut(s) | 796, 809 |
| Eco88I | CYCGRG | 1 cut(s) | 206 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 473 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1208, 1378 |
| EcoRI | GAATTC | 3 cut(s) | 38, 492, 1152 |
| EcoRII | CCWGG | 4 cut(s) | 199, 450, 1373, 1515 |
| EcoT14I | CCWWGG | 1 cut(s) | 933 |
| EgeI | GGCGCC | 1 cut(s) | 952 |
| EheI | GGCGCC | 1 cut(s) | 952 |
| ErhI | CCWWGG | 1 cut(s) | 933 |
| FaeI | CATG | 9 cut(s) | 106, 337, 907, 1045, 1145, 1200, 1294, 1390, 1526 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 152, 184, 794, 826 |
| FaqI | GGGAC | 2 cut(s) | 1119, 1221 |
| FatI | CATG | 9 cut(s) | 102, 333, 903, 1041, 1141, 1196, 1290, 1386, 1522 |
| FauI | CCCGC | 1 cut(s) | 637 |
| FbaI | TGATCA | 1 cut(s) | 849 |
| FblI | GTMKAC | 2 cut(s) | 180, 666 |
| Fnu4HI | GCNGC | 6 cut(s) | 87, 273, 381, 384, 792, 1039 |
| FokI | GGATG | 2 cut(s) | 609, 1174 |
| Fsp4HI | GCNGC | 6 cut(s) | 87, 273, 381, 384, 792, 1039 |
| FspBI | CTAG | 6 cut(s) | 90, 317, 611, 1052, 1214, 1247 |
| GlaI | GCGC | 1 cut(s) | 952 |
| GluI | GCNGC | 6 cut(s) | 87, 273, 381, 384, 792, 1039 |
| GsaI | CCCAGC | 2 cut(s) | 348, 1266 |
| GsuI | CTGGAG | 1 cut(s) | 900 |
| HaeII | RGCGCY | 1 cut(s) | 954 |
| HaeIII | GGCC | 3 cut(s) | 321, 779, 841 |
| HapII | CCGG | 4 cut(s) | 173, 1032, 1059, 1350 |
| HgaI | GACGC | 3 cut(s) | 285, 1399, 1517 |
| HhaI | GCGC | 1 cut(s) | 953 |
| Hin1I | GRCGYC | 2 cut(s) | 296, 951 |
| Hin1II | CATG | 9 cut(s) | 106, 337, 907, 1045, 1145, 1200, 1294, 1390, 1526 |
| Hin6I | GCGC | 1 cut(s) | 951 |
| HinP1I | GCGC | 1 cut(s) | 951 |
| HinfI | GANTC | 7 cut(s) | 99, 204, 441, 457, 891, 1411, 1485 |
| HpaII | CCGG | 4 cut(s) | 173, 1032, 1059, 1350 |
| HphI | GGTGA | 1 cut(s) | 1174 |
| Hpy166II | GTNNAC | 5 cut(s) | 181, 252, 303, 667, 1024 |
| Hpy188I | TCNGA | 4 cut(s) | 475, 734, 815, 1139 |
| Hpy8I | GTNNAC | 5 cut(s) | 181, 252, 303, 667, 1024 |
| Hpy99I | CGWCG | 2 cut(s) | 28, 301 |
| HpyAV | CCTTC | 4 cut(s) | 701, 820, 852, 1391 |
| HpyCH4III | ACNGT | 4 cut(s) | 249, 370, 646, 1003 |
| HpyCH4IV | ACGT | 1 cut(s) | 1301 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 219, 281, 334, 452, 1259 |
| HpyF3I | CTNAG | 2 cut(s) | 1369, 1416 |
| HpySE526I | ACGT | 1 cut(s) | 1301 |
| Hsp92I | GRCGYC | 2 cut(s) | 296, 951 |
| Hsp92II | CATG | 9 cut(s) | 106, 337, 907, 1045, 1145, 1200, 1294, 1390, 1526 |
| HspAI | GCGC | 1 cut(s) | 951 |
| KasI | GGCGCC | 1 cut(s) | 950 |
| Ksp22I | TGATCA | 1 cut(s) | 849 |
| LmnI | GCTCC | 1 cut(s) | 919 |
| Lsp1109I | GCAGC | 5 cut(s) | 98, 259, 367, 395, 778 |
| LweI | GCATC | 2 cut(s) | 390, 855 |
| MaeI | CTAG | 6 cut(s) | 90, 317, 611, 1052, 1214, 1247 |
| MaeII | ACGT | 1 cut(s) | 1301 |
| MaeIII | GTNAC | 3 cut(s) | 110, 136, 1077 |
| MflI | RGATCY | 4 cut(s) | 417, 729, 751, 805 |
| MhlI | GDGCHC | 1 cut(s) | 505 |
| MluCI | AATT | 7 cut(s) | 38, 164, 214, 492, 562, 881, 1152 |
| Mly113I | GGCGCC | 1 cut(s) | 951 |
| MlyI | GAGTC | 2 cut(s) | 93, 198 |
| MmeI | TCCRAC | 3 cut(s) | 418, 712, 901 |
| MroXI | GAANNNNTTC | 3 cut(s) | 164, 167, 1484 |
| MseI | TTAA | 5 cut(s) | 375, 507, 723, 942, 1116 |
| MspA1I | CMGCKG | 2 cut(s) | 523, 968 |
| MspI | CCGG | 4 cut(s) | 173, 1032, 1059, 1350 |
| MspR9I | CCNGG | 5 cut(s) | 173, 201, 452, 1375, 1517 |
| Mva1269I | GAATGC | 2 cut(s) | 540, 624 |
| MvaI | CCWGG | 4 cut(s) | 201, 452, 1375, 1517 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 219, 281, 334, 452, 1259 |
| NarI | GGCGCC | 1 cut(s) | 951 |
| NciI | CCSGG | 1 cut(s) | 173 |
| NlaIII | CATG | 9 cut(s) | 106, 337, 907, 1045, 1145, 1200, 1294, 1390, 1526 |
| NlaIV | GGNNCC | 6 cut(s) | 731, 753, 807, 952, 1209, 1379 |
| NmuCI | GTSAC | 1 cut(s) | 1077 |
| NspI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| NspV | TTCGAA | 1 cut(s) | 42 |
| PaeI | GCATGC | 2 cut(s) | 1045, 1526 |
| PaeR7I | CTCGAG | 1 cut(s) | 206 |
| PagI | TCATGA | 1 cut(s) | 1386 |
| PasI | CCCWGGG | 1 cut(s) | 1516 |
| PceI | AGGCCT | 1 cut(s) | 841 |
| PciI | ACATGT | 1 cut(s) | 1290 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 305 |
| PctI | GAATGC | 2 cut(s) | 540, 624 |
| PdmI | GAANNNNTTC | 3 cut(s) | 164, 167, 1484 |
| PfeI | GAWTC | 5 cut(s) | 441, 457, 891, 1411, 1485 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 934 |
| PkrI | GCNGC | 6 cut(s) | 88, 274, 382, 385, 793, 1040 |
| PleI | GAGTC | 2 cut(s) | 93, 198 |
| PluTI | GGCGCC | 1 cut(s) | 954 |
| PpsI | GAGTC | 2 cut(s) | 93, 198 |
| PpuMI | RGGWCCY | 2 cut(s) | 1208, 1378 |
| PscI | ACATGT | 1 cut(s) | 1290 |
| Psp5II | RGGWCCY | 2 cut(s) | 1208, 1378 |
| Psp6I | CCWGG | 4 cut(s) | 199, 450, 1373, 1515 |
| PspFI | CCCAGC | 2 cut(s) | 344, 1262 |
| PspGI | CCWGG | 4 cut(s) | 199, 450, 1373, 1515 |
| PspN4I | GGNNCC | 6 cut(s) | 731, 753, 807, 952, 1209, 1379 |
| PspPI | GGNCC | 2 cut(s) | 1208, 1378 |
| PspPPI | RGGWCCY | 2 cut(s) | 1208, 1378 |
| PstI | CTGCAG | 1 cut(s) | 385 |
| PsuI | RGATCY | 4 cut(s) | 417, 729, 751, 805 |
| PvuII | CAGCTG | 2 cut(s) | 523, 968 |
| RsaI | GTAC | 2 cut(s) | 266, 302 |
| RsaNI | GTAC | 2 cut(s) | 265, 301 |
| SaqAI | TTAA | 5 cut(s) | 375, 507, 723, 942, 1116 |
| SatI | GCNGC | 6 cut(s) | 87, 273, 381, 384, 792, 1039 |
| Sau96I | GGNCC | 2 cut(s) | 1208, 1378 |
| SchI | GAGTC | 2 cut(s) | 93, 198 |
| ScrFI | CCNGG | 5 cut(s) | 173, 201, 452, 1375, 1517 |
| SduI | GDGCHC | 1 cut(s) | 505 |
| SfaNI | GCATC | 2 cut(s) | 390, 855 |
| SfcI | CTRYAG | 3 cut(s) | 381, 645, 1174 |
| SfoI | GGCGCC | 1 cut(s) | 952 |
| Sfr274I | CTCGAG | 1 cut(s) | 206 |
| SfuI | TTCGAA | 1 cut(s) | 42 |
| SinI | GGWCC | 2 cut(s) | 1208, 1378 |
| SlaI | CTCGAG | 1 cut(s) | 206 |
| SmlI | CTYRAG | 1 cut(s) | 206 |
| SmoI | CTYRAG | 1 cut(s) | 206 |
| SphI | GCATGC | 2 cut(s) | 1045, 1526 |
| Sse9I | AATT | 7 cut(s) | 38, 164, 214, 492, 562, 881, 1152 |
| SseBI | AGGCCT | 1 cut(s) | 841 |
| SsiI | CCGC | 4 cut(s) | 5, 630, 794, 1039 |
| SspDI | GGCGCC | 1 cut(s) | 950 |
| SspI | AATATT | 1 cut(s) | 529 |
| SspMI | CTAG | 6 cut(s) | 90, 317, 611, 1052, 1214, 1247 |
| StuI | AGGCCT | 1 cut(s) | 841 |
| StyD4I | CCNGG | 5 cut(s) | 171, 199, 450, 1373, 1515 |
| StyI | CCWWGG | 1 cut(s) | 933 |
| TaaI | ACNGT | 4 cut(s) | 249, 370, 646, 1003 |
| TaiI | ACGT | 1 cut(s) | 1304 |
| TaqI | TCGA | 2 cut(s) | 42, 207 |
| TaqII | GACCGA | 1 cut(s) | 1467 |
| TasI | AATT | 7 cut(s) | 38, 164, 214, 492, 562, 881, 1152 |
| TauI | GCSGC | 1 cut(s) | 1041 |
| TfiI | GAWTC | 5 cut(s) | 441, 457, 891, 1411, 1485 |
| Tru1I | TTAA | 5 cut(s) | 375, 507, 723, 942, 1116 |
| Tru9I | TTAA | 5 cut(s) | 375, 507, 723, 942, 1116 |
| TscAI | CASTG | 2 cut(s) | 892, 1475 |
| TseFI | GTSAC | 1 cut(s) | 1077 |
| TseI | GCWGC | 5 cut(s) | 86, 272, 380, 383, 791 |
| Tsp45I | GTSAC | 1 cut(s) | 1077 |
| TspDTI | ATGAA | 1 cut(s) | 1477 |
| TspGWI | ACGGA | 1 cut(s) | 603 |
| TspRI | CASTG | 2 cut(s) | 892, 1475 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 934 |
| VpaK11BI | GGWCC | 2 cut(s) | 1208, 1378 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 473 |
| XapI | RAATTY | 4 cut(s) | 38, 164, 492, 1152 |
| XceI | RCATGY | 3 cut(s) | 1045, 1294, 1526 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 884 |
| XhoI | CTCGAG | 1 cut(s) | 206 |
| XmiI | GTMKAC | 2 cut(s) | 180, 666 |
| XmnI | GAANNNNTTC | 3 cut(s) | 164, 167, 1484 |
| XspI | CTAG | 6 cut(s) | 90, 317, 611, 1052, 1214, 1247 |