MD16G1189900.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000367
Physical Location & Seq
Reverse (-)
16541724 .. 16547374
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1189900.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGGCCGGCGGAGGGTTCTCGGCGGGTCCGGACGGGCGTGAGTTCGAGGCAAAGATCACGCCCATCGTCATCATCTCGTGTATAATGGCTGCTACTGGTGGACTCATGTTTGGTTACGATGTCGGTGTTTCAGGTGGAGTTACATCGATGCCCCCATTCTTGAAAAAGTTTTTCCCGGTTGTATACAGGAGGACTCTAGAACAGGGGATCAAAAGCAACTACTGCAAATACGACAACCAGGGCCTGCAGTTATTCACGTCGTCGCTGTACCTGGCCGGCTTAACTGCCACATTCGCCGCTTCCTACACCACCAGGAAGTTCGGGCGGAGGCCGTCTATGCTGATTGCTGGGATTTTCTTCATTGTCGGCACAATTCTCAATGCTGCAGCACAAGACCTCGCTATGCTCATCATTGGCAGGATCTCACTTGGTTGTGGAGTTGGGTTCGCAAATCAGGCTGTACCATTGTTTCTTTCGGAGATTGCACCGACAAGAATCCGCGGAGGGCTAAACATATTGTTCCAGCTTAATATCACAATAGGCATTCTATTTGCAAACCTCGTTAATTATGGCACCAACAAGATCACAGGAGGATGGGGTTGGAGGGTGTCATTGGGGCTGGCTGCTTTACCTGCCGGTCTCCTAACCGTGGGAGCTCTCTTGGTGGTCGAAACTCCTAACAGTCTCGTAGAACGTGGTCTCTTAGACCAAGGAAAATCCGTACTTAAAAGGATCCGCGGCACAGAAAATGTTGAACCAGAGTTCTTGGATCTTGTCGAGGCAAGTCGCATAGCAAAAGAAGTGAAGCACCCCTTTAGAAATCTCATAAAGCGCAGGAATCGTCCCCAACTAATCATTGCAGTAGCCTTGCAGATCTTTCAACAATTTACTGGGATCAATGCCATAATGTTTTACGCGCCAGTCCTGTTCGACACTTTAGGGTTTGGAAACGATGCTTCCCTCTACTCAGCTGTTATCACAGGAGCCGTCAATGTTCTCTCCACCGTTGTATCCATCTACTCAGTTGACAAAGTAGGCCGCCGCATGCTCTTACTTCAAGCTGGTTTTCAAATGTTCATCTCTCAAGTGGCTATTGCCATAATACTAGGGATCAAGGTTAAGGACCACTCCGATGACCTCCACAAAGGCTTCGCAATCTTTGTGGTGGTTCTGATTTGCACTTACGTTGCTGCGTTTGCTTGGTCTTGGGGGCCTCTAGGTTGGTTGATACCAAGTGAGACATTCCCACTCGAGACTCGCTCAGCGGGGCAAAGTGTGACTGTCTGCACCAACTTGCTCTTTACTTTCATTATAGCGCAGGGCTTCCTCTCGATGCTCTGCCACTTCAAGTACGGCATCTTCCTCTTCTTCTCCGGGTGGGTCTTGATCATGTCTTTCTTCGTGTTGTTCCTACTCCCGGAGACGAAGAACATTCCGATTGAAGAGATGACAGAGAGGGTGTGGAAGCAACATTGGTTGTGGAAGAGGTTTATGGATGACGATGACTACCACATTGAAGGAAATAATGGTGATGGCTTAAAGAAAAATGGGCACGCTAATGGATTTGATGGTGTTTCTCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

529

Amino Acids

57.96

Weight (kDa)

9.16

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 27 - 485 2.7e-123 Sugar (and other) transporter
MFS_1 PF07690 79 - 431 4.9e-24 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26340
fragaria_vesca FvH4_2g38270 FvH4_4g15150 FvH4_4g15160 FvH4_4g15170 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15172 FvH4_4g15180 FvH4_4g15220 FvH4_4g15221
malus_domestica MD13G1189100.v1.1 MD13G1189500.v1.1 MD13G1189600.v1.1 MD13G1189800.v1.1 MD13G1190100.v1.1 MD13G1190200.v1.1 MD13G1190300.v1.1 MD16G1189900.v1.1 MD16G1190000.v1.1 MD16G1190100.v1.1 MD16G1190300.v1.1
prunus_persica Prupe.1G156300_v2.0.a1 Prupe.1G156300_v2.0.a1 Prupe.1G156400_v2.0.a1 Prupe.1G156500_v2.0.a1 Prupe.1G156600_v2.0.a1 Prupe.1G156700_v2.0.a1 Prupe.1G156800_v2.0.a1 Prupe.1G156900_v2.0.a1 Prupe.1G157000_v2.0.a1
pyrus_communis pycom13g16350 pycom13g16370 pycom13g16380 pycom16g15990 pycom16g16010 pycom16g16020
rosa_chinensis RchiOBHm_Chr4g0413631 RchiOBHm_Chr4g0416271 RchiOBHm_Chr4g0416631 RchiOBHm_Chr4g0416651 RchiOBHm_Chr4g0416671 RchiOBHm_Chr4g0416681 RchiOBHm_Chr4g0416691 RchiOBHm_Chr4g0416721
rosa_laevigata RLG00000007958 RLG00000007959 RLG00000007960 RLG00000007961 RLG00000007963 RLG00000007964 RLG00000008204
rosa_multiflora Rmu_ssc0000377.1_g000001 Rmu_ssc0000377.1_g000007 Rmu_ssc0000377.1_g000017 Rmu_ssc0000377.1_g000025 Rmu_ssc0000377.1_g000041 Rmu_ssc0000377.1_g000043
rosa_roxburghii Rroxscaffold_5G00357580 Rroxscaffold_5G00360530 Rroxscaffold_5G00360560 Rroxscaffold_5G00360580 Rroxscaffold_5G00360590
rosa_rugosa Rorug04G0121900 Rorug04G0143800 Rorug04G0143900 Rorug04G0144000 Rorug04G0144000 Rorug04G0144100
rosa_samantha Rh4AG181300 Rh4AG206000 Rh4AG206100 Rh4AG206200 Rh4AG206300 Rh4AG206500 Rh4AG206700 Rh4BG180600 Rh4BG203400 Rh4BG203500 Rh4BG203600 Rh4BG203700 Rh4BG203800 Rh4BG203900 Rh4BG204000 Rh4CG192500 Rh4CG216900 Rh4CG217200 Rh4CG217500 Rh4CG217700 Rh4CG217800 Rh4DG176700 Rh4DG202900 Rh4DG203000 Rh4DG203200 Rh4DG203300 Rh4DG203900
rosa_wichuraiana Rw4G015160 Rw4G017430 Rw4G017440 Rw4G017450 Rw4G017460 Rw4G017480 Rw4G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 572
AccI GTMKAC 1 cut(s) 183
AccII CGCG 3 cut(s) 501, 738, 917
AccIII TCCGGA 1 cut(s) 28
AclWI GGATC 7 cut(s) 215, 428, 727, 740, 777, 902, 1118
AcoI YGGCCR 2 cut(s) 3, 273
AfaI GTAC 4 cut(s) 269, 462, 723, 1352
AfiI CCNNNNNNNGG 3 cut(s) 12, 649, 1417
AgsI TTSAA 8 cut(s) 163, 755, 881, 1058, 1070, 1348, 1442, 1517
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 3 cut(s) 237, 270, 311
AluBI AGCT 4 cut(s) 526, 656, 971, 1061
AluI AGCT 4 cut(s) 526, 656, 971, 1061
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
AlwI GGATC 7 cut(s) 215, 428, 727, 740, 777, 902, 1118
AlwNI CAGNNNCTG 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 1250
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 6 cut(s) 3, 241, 273, 329, 1036, 1211
ApeKI GCWGC 5 cut(s) 89, 383, 386, 623, 1190
AspLEI GCGC 3 cut(s) 834, 919, 1318
AspS9I GGNCC 4 cut(s) 26, 241, 1123, 1211
AsuC2I CCSGG 3 cut(s) 176, 1375, 1418
AsuHPI GGTGA 1 cut(s) 1541
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 2 cut(s) 26, 1123
BaeGI GKGCMC 1 cut(s) 1554
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BamHI GGATCC 1 cut(s) 732
BanI GGYRCC 1 cut(s) 572
BanII GRGCYC 1 cut(s) 658
BarI GAAGNNNNNNTAC 2 cut(s) 1343, 1375
BauI CACGAG 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 5 cut(s) 76, 370, 398, 610, 1177
BccI CCATC 5 cut(s) 71, 588, 1022, 1526, 1562
BceAI ACGGC 3 cut(s) 316, 971, 1369
BciT130I CCWGG 3 cut(s) 239, 272, 313
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 1386
BcnI CCSGG 3 cut(s) 176, 1375, 1418
BcoDI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BfaI CTAG 3 cut(s) 197, 1106, 1217
BfmI CTRYAG 2 cut(s) 245, 384
BfuAI ACCTGC 1 cut(s) 640
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 1 cut(s) 873
BisI GCNGC 9 cut(s) 90, 297, 384, 387, 624, 739, 1039, 1042, 1191
BlpI GCTNAGC 1 cut(s) 1261
BlsI GCNGC 9 cut(s) 91, 298, 385, 388, 625, 740, 1040, 1043, 1192
Bme1390I CCNGG 6 cut(s) 176, 239, 272, 313, 1375, 1418
Bme18I GGWCC 2 cut(s) 26, 1123
BmeT110I CYCGRG 1 cut(s) 1250
BmgBI CACGTC 1 cut(s) 258
BmgT120I GGNCC 4 cut(s) 26, 241, 1123, 1211
BmiI GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
BmrFI CCNGG 6 cut(s) 176, 239, 272, 313, 1375, 1418
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 4 cut(s) 138, 943, 1323, 1365
BmuI ACTGGG 1 cut(s) 900
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
Bpu1102I GCTNAGC 1 cut(s) 1261
BpuEI CTTGAG 1 cut(s) 1068
BpuMI CCSGG 3 cut(s) 176, 1375, 1418
Bsa29I ATCGAT 1 cut(s) 146
BsaI GGTCTC 2 cut(s) 644, 704
BsaJI CCNNGG 5 cut(s) 238, 499, 648, 709, 736
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 649, 1417
Bse118I RCCGGY 3 cut(s) 5, 275, 635
Bse1I ACTGG 3 cut(s) 100, 895, 920
Bse3DI GCAATG 1 cut(s) 855
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 3 cut(s) 239, 272, 313
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 5 cut(s) 238, 499, 648, 709, 736
BseGI GGATG 2 cut(s) 599, 1501
BseLI CCNNNNNNNGG 3 cut(s) 12, 649, 1417
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 3 cut(s) 981, 1035, 1275
BseNI ACTGG 3 cut(s) 100, 895, 920
BseSI GKGCMC 1 cut(s) 1554
BseXI GCAGC 5 cut(s) 76, 370, 398, 610, 1177
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 1270
Bsh1236I CGCG 3 cut(s) 501, 738, 917
BshFI GGCC 6 cut(s) 5, 243, 275, 331, 1038, 1213
BshNI GGYRCC 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 7 cut(s) 6, 29, 176, 276, 636, 1374, 1418
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 3 cut(s) 12, 649, 1417
BsmAI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BsmBI CGTCTC 1 cut(s) 1415
BsmFI GGGAC 1 cut(s) 828
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 6 cut(s) 5, 243, 275, 331, 1038, 1213
Bso31I GGTCTC 2 cut(s) 644, 704
BsoBI CYCGRG 1 cut(s) 1250
Bsp1286I GDGCHC 2 cut(s) 658, 1554
Bsp13I TCCGGA 1 cut(s) 28
Bsp1720I GCTNAGC 1 cut(s) 1261
BspANI GGCC 6 cut(s) 5, 243, 275, 331, 1038, 1213
BspCNI CTCAG 3 cut(s) 980, 1034, 1274
BspDI ATCGAT 1 cut(s) 146
BspEI TCCGGA 1 cut(s) 28
BspFNI CGCG 3 cut(s) 501, 738, 917
BspLI GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
BspMAI CTGCAG 2 cut(s) 249, 388
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 7 cut(s) 215, 428, 727, 740, 777, 902, 1118
BspT107I GGYRCC 1 cut(s) 572
BspTNI GGTCTC 2 cut(s) 644, 704
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 3 cut(s) 5, 275, 635
BsrI ACTGG 3 cut(s) 100, 895, 920
BssAI RCCGGY 3 cut(s) 5, 275, 635
BssECI CCNNGG 5 cut(s) 238, 499, 648, 709, 736
BssNAI GTATAC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 709
Bst1107I GTATAC 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 239, 272, 313
Bst4CI ACNGT 4 cut(s) 649, 683, 1006, 1282
Bst6I CTCTTC 3 cut(s) 1370, 1437, 1478
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 6 cut(s) 7, 245, 277, 621, 1046, 1554
BstDEI CTNAG 5 cut(s) 703, 967, 1021, 1261, 1578
BstDSI CCRYGG 3 cut(s) 499, 648, 736
BstF5I GGATG 2 cut(s) 599, 1501
BstFNI CGCG 3 cut(s) 501, 738, 917
BstHHI GCGC 3 cut(s) 834, 919, 1318
BstMAI GTCTC 6 cut(s) 644, 689, 704, 1232, 1247, 1415
BstMWI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 632, 1196
BstNI CCWGG 3 cut(s) 239, 272, 313
BstNSI RCATGY 1 cut(s) 1048
BstSCI CCNGG 6 cut(s) 174, 237, 270, 311, 1373, 1416
BstSFI CTRYAG 2 cut(s) 245, 384
BstSLI GKGCMC 1 cut(s) 1554
BstUI CGCG 3 cut(s) 501, 738, 917
BstV1I GCAGC 5 cut(s) 76, 370, 398, 610, 1177
BstX2I RGATCY 4 cut(s) 420, 732, 769, 873
BstYI RGATCY 4 cut(s) 420, 732, 769, 873
BstZ17I GTATAC 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 146
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 6 cut(s) 5, 243, 275, 331, 1038, 1213
BsuTUI ATCGAT 1 cut(s) 146
BtgI CCRYGG 3 cut(s) 499, 648, 736
BtrI CACGTC 1 cut(s) 258
BtsCI GGATG 2 cut(s) 599, 1501
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 6 cut(s) 7, 245, 277, 621, 1046, 1554
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 3 cut(s) 834, 919, 1318
Cfr10I RCCGGY 3 cut(s) 5, 275, 635
Cfr13I GGNCC 4 cut(s) 26, 241, 1123, 1211
Cfr42I CCGCGG 2 cut(s) 502, 739
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 4 cut(s) 268, 461, 722, 1351
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviAII CATG 3 cut(s) 106, 1045, 1390
CviQI GTAC 4 cut(s) 268, 461, 722, 1351
DdeI CTNAG 5 cut(s) 703, 967, 1021, 1261, 1578
EaeI YGGCCR 2 cut(s) 3, 273
Eam1104I CTCTTC 3 cut(s) 1370, 1437, 1478
EarI CTCTTC 3 cut(s) 1370, 1437, 1478
EciI GGCGGA 2 cut(s) 24, 340
Ecl136II GAGCTC 1 cut(s) 656
Eco130I CCWWGG 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 2 cut(s) 644, 704
Eco47I GGWCC 2 cut(s) 26, 1123
Eco53kI GAGCTC 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 1250
EcoICRI GAGCTC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 241, 1211
EcoRII CCWGG 3 cut(s) 237, 270, 311
EcoT14I CCWWGG 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 709
Esp3I CGTCTC 1 cut(s) 1415
FaeI CATG 3 cut(s) 109, 1048, 1393
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FaqI GGGAC 1 cut(s) 828
FatI CATG 3 cut(s) 105, 1044, 1389
FauI CCCGC 2 cut(s) 16, 1258
FbaI TGATCA 1 cut(s) 1386
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 9 cut(s) 90, 297, 384, 387, 624, 739, 1039, 1042, 1191
FokI GGATG 2 cut(s) 606, 1508
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 9 cut(s) 90, 297, 384, 387, 624, 739, 1039, 1042, 1191
FspBI CTAG 3 cut(s) 197, 1106, 1217
GlaI GCGC 3 cut(s) 833, 918, 1317
GluI GCNGC 9 cut(s) 90, 297, 384, 387, 624, 739, 1039, 1042, 1191
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 6 cut(s) 5, 243, 275, 331, 1038, 1213
HapII CCGG 7 cut(s) 6, 29, 176, 276, 636, 1374, 1418
HhaI GCGC 3 cut(s) 834, 919, 1318
Hin1II CATG 3 cut(s) 109, 1048, 1393
Hin6I GCGC 3 cut(s) 832, 917, 1316
HinP1I GCGC 3 cut(s) 832, 917, 1316
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 5 cut(s) 102, 193, 495, 838, 1255
HpaII CCGG 7 cut(s) 6, 29, 176, 276, 636, 1374, 1418
HphI GGTGA 1 cut(s) 1541
Hpy166II GTNNAC 3 cut(s) 101, 184, 1027
Hpy188I TCNGA 4 cut(s) 478, 1132, 1173, 1437
Hpy188III TCNNGA 6 cut(s) 29, 160, 197, 1252, 1330, 1384
Hpy8I GTNNAC 3 cut(s) 101, 184, 1027
Hpy99I CGWCG 2 cut(s) 262, 265
HpyAV CCTTC 1 cut(s) 1511
HpyCH4III ACNGT 4 cut(s) 649, 683, 1006, 1282
HpyCH4IV ACGT 3 cut(s) 257, 694, 1185
HpyCH4V TGCA 9 cut(s) 225, 247, 386, 485, 554, 860, 871, 1179, 1287
HpyF10VI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 632, 1196
HpyF3I CTNAG 5 cut(s) 703, 967, 1021, 1261, 1578
HpySE526I ACGT 3 cut(s) 257, 694, 1185
Hsp92II CATG 3 cut(s) 109, 1048, 1393
HspAI GCGC 3 cut(s) 832, 917, 1316
Kpn2I TCCGGA 1 cut(s) 28
KroI GCCGGC 2 cut(s) 5, 275
KroNI GCCGGC 2 cut(s) 7, 277
Ksp22I TGATCA 1 cut(s) 1386
KspI CCGCGG 2 cut(s) 502, 739
LmnI GCTCC 2 cut(s) 653, 983
Lsp1109I GCAGC 5 cut(s) 76, 370, 398, 610, 1177
LweI GCATC 4 cut(s) 138, 943, 1323, 1365
MaeI CTAG 3 cut(s) 197, 1106, 1217
MaeII ACGT 3 cut(s) 257, 694, 1185
MaeIII GTNAC 3 cut(s) 113, 139, 1276
MboII GAAGA 8 cut(s) 349, 1351, 1357, 1360, 1390, 1438, 1454, 1495
MflI RGATCY 4 cut(s) 420, 732, 769, 873
MhlI GDGCHC 2 cut(s) 658, 1554
MluCI AATT 3 cut(s) 372, 565, 884
MlyI GAGTC 3 cut(s) 96, 187, 1249
MmeI TCCRAC 1 cut(s) 581
MroI TCCGGA 1 cut(s) 28
MroNI GCCGGC 2 cut(s) 5, 275
MseI TTAA 6 cut(s) 281, 528, 564, 726, 1119, 1538
MslI CAYNNNNRTG 2 cut(s) 1131, 1557
MspA1I CMGCKG 4 cut(s) 501, 738, 971, 1265
MspI CCGG 7 cut(s) 6, 29, 176, 276, 636, 1374, 1418
MspR9I CCNGG 6 cut(s) 176, 239, 272, 313, 1375, 1418
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 3 cut(s) 239, 272, 313
MvnI CGCG 3 cut(s) 501, 738, 917
MwoI GCNNNNNNNGC 6 cut(s) 222, 293, 337, 455, 632, 1196
NaeI GCCGGC 2 cut(s) 7, 277
NciI CCSGG 3 cut(s) 176, 1375, 1418
NgoMIV GCCGGC 2 cut(s) 5, 275
NlaIII CATG 3 cut(s) 109, 1048, 1393
NlaIV GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
NmuCI GTSAC 1 cut(s) 1276
NspI RCATGY 1 cut(s) 1048
PaeI GCATGC 1 cut(s) 1048
PaeR7I CTCGAG 1 cut(s) 1250
PctI GAATGC 1 cut(s) 543
PdiI GCCGGC 2 cut(s) 7, 277
PfeI GAWTC 2 cut(s) 495, 838
PfoI TCCNGGA 1 cut(s) 1416
PkrI GCNGC 9 cut(s) 91, 298, 385, 388, 625, 740, 1040, 1043, 1192
PleI GAGTC 3 cut(s) 96, 187, 1249
PpsI GAGTC 3 cut(s) 96, 187, 1249
Psp124BI GAGCTC 1 cut(s) 658
Psp6I CCWGG 3 cut(s) 237, 270, 311
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 237, 270, 311
PspN4I GGNNCC 5 cut(s) 27, 574, 734, 985, 1212
PspPI GGNCC 4 cut(s) 26, 241, 1123, 1211
PstI CTGCAG 2 cut(s) 249, 388
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 4 cut(s) 420, 732, 769, 873
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 4 cut(s) 269, 462, 723, 1352
RsaNI GTAC 4 cut(s) 268, 461, 722, 1351
RseI CAYNNNNRTG 2 cut(s) 1131, 1557
SacI GAGCTC 1 cut(s) 658
SacII CCGCGG 2 cut(s) 502, 739
SaqAI TTAA 6 cut(s) 281, 528, 564, 726, 1119, 1538
SatI GCNGC 9 cut(s) 90, 297, 384, 387, 624, 739, 1039, 1042, 1191
Sau96I GGNCC 4 cut(s) 26, 241, 1123, 1211
SchI GAGTC 3 cut(s) 96, 187, 1249
ScrFI CCNGG 6 cut(s) 176, 239, 272, 313, 1375, 1418
SduI GDGCHC 2 cut(s) 658, 1554
SfaNI GCATC 4 cut(s) 138, 943, 1323, 1365
SfcI CTRYAG 2 cut(s) 245, 384
Sfr274I CTCGAG 1 cut(s) 1250
Sfr303I CCGCGG 2 cut(s) 502, 739
SgrBI CCGCGG 2 cut(s) 502, 739
SinI GGWCC 2 cut(s) 26, 1123
SlaI CTCGAG 1 cut(s) 1250
SmiMI CAYNNNNRTG 2 cut(s) 1131, 1557
SmlI CTYRAG 2 cut(s) 1083, 1250
SmoI CTYRAG 2 cut(s) 1083, 1250
SphI GCATGC 1 cut(s) 1048
Sse9I AATT 3 cut(s) 372, 565, 884
SspMI CTAG 3 cut(s) 197, 1106, 1217
SstI GAGCTC 1 cut(s) 658
StyD4I CCNGG 6 cut(s) 174, 237, 270, 311, 1373, 1416
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 4 cut(s) 649, 683, 1006, 1282
TaiI ACGT 3 cut(s) 260, 697, 1188
TaqI TCGA 7 cut(s) 45, 146, 669, 777, 930, 1251, 1331
TasI AATT 3 cut(s) 372, 565, 884
TauI GCSGC 4 cut(s) 299, 741, 1041, 1044
TfiI GAWTC 2 cut(s) 495, 838
Tru1I TTAA 6 cut(s) 281, 528, 564, 726, 1119, 1538
Tru9I TTAA 6 cut(s) 281, 528, 564, 726, 1119, 1538
TseFI GTSAC 1 cut(s) 1276
TseI GCWGC 5 cut(s) 89, 383, 386, 623, 1190
Tsp45I GTSAC 1 cut(s) 1276
TspDTI ATGAA 3 cut(s) 349, 1066, 1297
TspGWI ACGGA 1 cut(s) 709
VpaK11BI GGWCC 2 cut(s) 26, 1123
XbaI TCTAGA 1 cut(s) 196
XceI RCATGY 1 cut(s) 1048
XhoI CTCGAG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 3 cut(s) 197, 1106, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.