AT5G39410

Saccharopine dehydrogenase NADP binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002135
Physical Location & Seq
Reverse (-)
15767898 .. 15770456
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39410.1

Sequence Viewer

Length: 1365 bp
ATGAACCCAACCCAAAAACCCGAACCGGTTTACGATATGGTCATACTCGGAGCATCCGGATTTACCGGTAAGTACGTCGTCAGAGAAGCTCTCAAGTTCCTTCAAACACCGTCTTCTTCTCCGTTAAAGTCTCTAGCTTTAGCGGGTCGTAACCCGACCCGTTTAACCCAATCTCTCGAATGGGCCGCCCGCCCGAACCCACCACCTTCCTCTGTCGCTATCCTCACTGCTGATACATCTGACCCTGATTCACTTCGTCGTCTCTGTACTCAAACCAAACTCATCCTCAATTGTGTTGGACCGTTTCGTATCCATGGTGATCCTGTCGTCTCTGCTTGTGCTGATTCAGGGTGTGATTATTTGGATATAAGTGGTGAACCTGAGTTTATGGAGAGAATGGAAGCTAACTACCATGATAGAGCAGAAGAGACTGGCTCTTTAATCGTTTCTGCTTGTGGTTTTGATTCAATTCCTGCTGAATTGGGTCTTCTCTTTAATGCTAAACAATGGGTATCTCCATCGGTTCCTAACCAGATTGAAGCGTACCTTAGCTTGGAGTCTGACAAAAAAATTGCTGGGAACTTTGGGACTTATGAGTCTGCGGTTTTAGGTGTTGCTAATGCAGAAAAGCTTAAAGAATTAAGACGTTCAAGACCAAGAAGGCCAAGACCAACGATTTGTGGTCCTCCTGCTAAAGGACCAACATTAGAAAACCAGAAGACGATTGGTCTTTGGGCTTTAAAGCTACCTTCAGCTGATGCAGTAGTTGTTCGTAGAACTCTCACAACTCTAACAGAGAAACCACATGGGCTTCCTGGGATTAATGAAAGTCCTGAGCAGATACAAAAGAGAGAAGCATTCTGGTCATCGATCAAGCCTGCTCATTTTGGTGTAAAGATAACGTCCAAATCTCTCTTTGGGATATTCCGATATGTTACACTTGGAGTGTCACTTGGTTTACTTTCCAAGTTCTCCTTCGGAAGATGGCTTCTTTTGAAATTCCCTTCAGTTTTCAGCCTTGGTTGGTTCCAGAAGAAAGGTCCAAGTGAAGAAGAGGTAGAAAGCGCTACGTTTAAGATGTGGTTCATAGGTCGTGGGTACAGCGAAGAGAGTCTAGCTTCACAAGGAGAAACAAAGCCTGACTTGGAAATCATTACAAGAATTTCAGGACCTGAGATTGGATATATAACCACCCCGATAACACTTGTTCAATGCGGTTTGATAGTCTTGGGCCAGCGCGAAAGCCTAGTTAAAGGAGGAGTCTACACACCCGGCATTGTGTTTGGTTCAACCGATATCCAGCAGCGACTTGAGGATAATGGTATATCTTTTGAGCTGATTTCAAAGATCAAGACTCAAGGATAA

Protein Analysis

454

Amino Acids

49.68

Weight (kDa)

8.54

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 14 - 149 4.3e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 1263
AccII CGCG 1 cut(s) 1239
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 5 cut(s) 143, 186, 190, 602, 1215
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 2 cut(s) 998, 1161
AcuI CTGAAG 2 cut(s) 735, 990
AfaI GTAC 4 cut(s) 74, 268, 545, 1100
AfeI AGCGCT 1 cut(s) 1066
AfiI CCNNNNNNNGG 3 cut(s) 553, 695, 1178
AgeI ACCGGT 2 cut(s) 25, 65
AgsI TTSAA 8 cut(s) 104, 468, 539, 651, 997, 1211, 1290, 1344
AhdI GACNNNNNGTC 1 cut(s) 726
AjnI CCWGG 1 cut(s) 814
AjuI GAANNNNNNNTTGG 2 cut(s) 959, 991
AluBI AGCT 9 cut(s) 89, 137, 404, 552, 631, 745, 755, 1118, 1336
AluI AGCT 9 cut(s) 89, 137, 404, 552, 631, 745, 755, 1118, 1336
Alw26I GTCTC 4 cut(s) 135, 266, 334, 422
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 1172
Aor13HI TCCGGA 1 cut(s) 56
Aor51HI AGCGCT 1 cut(s) 1066
AoxI GGCC 3 cut(s) 183, 662, 1231
ApeKI GCWGC 1 cut(s) 1303
ApoI RAATTY 2 cut(s) 998, 1161
ArsI GACNNNNNNTTYG 2 cut(s) 660, 692
AseI ATTAAT 1 cut(s) 822
AsiGI ACCGGT 2 cut(s) 25, 65
AspLEI GCGC 2 cut(s) 1067, 1239
AspS9I GGNCC 7 cut(s) 183, 299, 683, 698, 1040, 1169, 1231
AsuC2I CCSGG 1 cut(s) 1272
AsuHPI GGTGA 2 cut(s) 329, 386
AvaII GGWCC 5 cut(s) 299, 683, 698, 1040, 1169
BbsI GAAGAC 3 cut(s) 105, 479, 725
BbvI GCAGC 1 cut(s) 1315
BccI CCATC 2 cut(s) 526, 978
BciT130I CCWGG 1 cut(s) 816
BciVI GTATCC 1 cut(s) 320
BcnI CCSGG 1 cut(s) 1272
BcoDI GTCTC 4 cut(s) 135, 266, 334, 422
BfaI CTAG 3 cut(s) 134, 1115, 1247
BfoI RGCGCY 1 cut(s) 1068
BfuI GTATCC 1 cut(s) 320
BisI GCNGC 2 cut(s) 186, 1304
BlsI GCNGC 2 cut(s) 187, 1305
Bme1390I CCNGG 2 cut(s) 816, 1272
Bme18I GGWCC 5 cut(s) 299, 683, 698, 1040, 1169
BmeRI GACNNNNNGTC 1 cut(s) 726
BmgT120I GGNCC 7 cut(s) 183, 299, 683, 698, 1040, 1169, 1231
BmiI GGNNCC 2 cut(s) 525, 1028
BmrFI CCNGG 2 cut(s) 816, 1272
BmsI GCATC 2 cut(s) 62, 748
BpiI GAAGAC 3 cut(s) 105, 479, 725
BplI GAGNNNNNCTC 4 cut(s) 75, 107, 419, 451
Bpu10I CCTNAGC 2 cut(s) 548, 834
BpuEI CTTGAG 3 cut(s) 77, 1331, 1341
BpuMI CCSGG 1 cut(s) 1272
Bsa29I ATCGAT 1 cut(s) 869
BsaJI CCNNGG 3 cut(s) 313, 815, 1018
BsaWI WCCGGW 3 cut(s) 25, 56, 65
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 695, 1178
Bse118I RCCGGY 2 cut(s) 25, 65
Bse1I ACTGG 1 cut(s) 436
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 1 cut(s) 816
BseCI ATCGAT 1 cut(s) 869
BseDI CCNNGG 3 cut(s) 313, 815, 1018
BseGI GGATG 2 cut(s) 53, 282
BseLI CCNNNNNNNGG 3 cut(s) 553, 695, 1178
BseMII CTCAG 3 cut(s) 372, 825, 1164
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 1 cut(s) 1272
BseXI GCAGC 1 cut(s) 1315
BseYI CCCAGC 1 cut(s) 575
Bsh1236I CGCG 1 cut(s) 1239
BshFI GGCC 3 cut(s) 185, 664, 1233
BshTI ACCGGT 2 cut(s) 25, 65
BshVI ATCGAT 1 cut(s) 869
BsiSI CCGG 4 cut(s) 26, 57, 66, 1272
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 3 cut(s) 553, 695, 1178
BsmAI GTCTC 4 cut(s) 135, 266, 334, 422
BsmBI CGTCTC 2 cut(s) 266, 334
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 3 cut(s) 185, 664, 1233
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 3 cut(s) 319, 870, 1347
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 5 cut(s) 143, 186, 190, 602, 1215
BspANI GGCC 3 cut(s) 185, 664, 1233
BspCNI CTCAG 3 cut(s) 373, 826, 1165
BspDI ATCGAT 1 cut(s) 869
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 1 cut(s) 1239
BspLI GGNNCC 2 cut(s) 525, 1028
BspPI GGATC 1 cut(s) 314
BsrFI RCCGGY 2 cut(s) 25, 65
BsrI ACTGG 1 cut(s) 436
BssAI RCCGGY 2 cut(s) 25, 65
BssECI CCNNGG 3 cut(s) 313, 815, 1018
BssMI GATC 3 cut(s) 319, 870, 1347
BssT1I CCWWGG 2 cut(s) 313, 1018
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 2 cut(s) 111, 303
Bst6I CTCTTC 3 cut(s) 420, 1047, 1101
BstC8I GCNNGC 3 cut(s) 190, 879, 1235
BstDEI CTNAG 4 cut(s) 381, 548, 834, 1173
BstDSI CCRYGG 1 cut(s) 313
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 2 cut(s) 53, 282
BstFNI CGCG 1 cut(s) 1239
BstH2I RGCGCY 1 cut(s) 1068
BstHHI GCGC 2 cut(s) 1067, 1239
BstKTI GATC 3 cut(s) 322, 873, 1350
BstMAI GTCTC 4 cut(s) 135, 266, 334, 422
BstMBI GATC 3 cut(s) 319, 870, 1347
BstNI CCWGG 1 cut(s) 816
BstSCI CCNGG 2 cut(s) 814, 1270
BstUI CGCG 1 cut(s) 1239
BstV1I GCAGC 1 cut(s) 1315
BstV2I GAAGAC 3 cut(s) 105, 479, 725
Bsu15I ATCGAT 1 cut(s) 869
BsuI GTATCC 1 cut(s) 320
BsuRI GGCC 3 cut(s) 185, 664, 1233
BsuTUI ATCGAT 1 cut(s) 869
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 53, 282
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 3 cut(s) 190, 879, 1235
CaiI CAGNNNCTG 1 cut(s) 1172
CfoI GCGC 2 cut(s) 1067, 1239
Cfr10I RCCGGY 2 cut(s) 25, 65
Cfr13I GGNCC 7 cut(s) 183, 299, 683, 698, 1040, 1169, 1231
ClaI ATCGAT 1 cut(s) 869
Csp6I GTAC 4 cut(s) 73, 267, 544, 1099
CspAI ACCGGT 2 cut(s) 25, 65
CviAII CATG 3 cut(s) 314, 413, 806
CviQI GTAC 4 cut(s) 73, 267, 544, 1099
DdeI CTNAG 4 cut(s) 381, 548, 834, 1173
DpnI GATC 3 cut(s) 321, 872, 1349
DpnII GATC 3 cut(s) 319, 870, 1347
DraI TTTAAA 1 cut(s) 741
DrdI GACNNNNNNGTC 1 cut(s) 595
DriI GACNNNNNGTC 1 cut(s) 726
DseDI GACNNNNNNGTC 1 cut(s) 595
Eam1104I CTCTTC 3 cut(s) 420, 1047, 1101
Eam1105I GACNNNNNGTC 1 cut(s) 726
EarI CTCTTC 3 cut(s) 420, 1047, 1101
Eco130I CCWWGG 2 cut(s) 313, 1018
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 5 cut(s) 299, 683, 698, 1040, 1169
Eco47III AGCGCT 1 cut(s) 1066
Eco57I CTGAAG 2 cut(s) 735, 990
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRII CCWGG 1 cut(s) 814
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 2 cut(s) 313, 1018
ErhI CCWWGG 2 cut(s) 313, 1018
Esp3I CGTCTC 2 cut(s) 266, 334
FaeI CATG 3 cut(s) 317, 416, 809
FalI AAGNNNNNCTT 6 cut(s) 531, 563, 959, 991, 1127, 1159
FaqI GGGAC 1 cut(s) 601
FatI CATG 3 cut(s) 313, 412, 805
FauI CCCGC 2 cut(s) 136, 197
FblI GTMKAC 1 cut(s) 1263
Fnu4HI GCNGC 2 cut(s) 186, 1304
FokI GGATG 2 cut(s) 40, 269
Fsp4HI GCNGC 2 cut(s) 186, 1304
FspBI CTAG 3 cut(s) 134, 1115, 1247
GlaI GCGC 2 cut(s) 1066, 1238
GluI GCNGC 2 cut(s) 186, 1304
GsaI CCCAGC 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 1068
HaeIII GGCC 3 cut(s) 185, 664, 1233
HapII CCGG 4 cut(s) 26, 57, 66, 1272
HhaI GCGC 2 cut(s) 1067, 1239
Hin1II CATG 3 cut(s) 317, 416, 809
Hin6I GCGC 2 cut(s) 1065, 1237
HinP1I GCGC 2 cut(s) 1065, 1237
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 8 cut(s) 248, 344, 464, 557, 596, 1111, 1260, 1354
HpaII CCGG 4 cut(s) 26, 57, 66, 1272
HphI GGTGA 2 cut(s) 329, 386
Hpy166II GTNNAC 4 cut(s) 31, 377, 959, 1264
Hpy188I TCNGA 6 cut(s) 50, 83, 241, 562, 929, 980
Hpy188III TCNNGA 7 cut(s) 57, 176, 651, 833, 1030, 1167, 1351
Hpy8I GTNNAC 4 cut(s) 31, 377, 959, 1264
Hpy99I CGWCG 2 cut(s) 80, 261
HpyAV CCTTC 6 cut(s) 110, 216, 654, 759, 985, 1014
HpyCH4III ACNGT 2 cut(s) 111, 303
HpyCH4IV ACGT 4 cut(s) 75, 646, 902, 1070
HpyCH4V TGCA 2 cut(s) 623, 761
HpyF3I CTNAG 4 cut(s) 381, 548, 834, 1173
HpySE526I ACGT 4 cut(s) 75, 646, 902, 1070
Hsp92II CATG 3 cut(s) 317, 416, 809
HspAI GCGC 2 cut(s) 1065, 1237
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 3 cut(s) 319, 870, 1347
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 1 cut(s) 1315
LweI GCATC 2 cut(s) 62, 748
MaeI CTAG 3 cut(s) 134, 1115, 1247
MaeII ACGT 4 cut(s) 75, 646, 902, 1070
MaeIII GTNAC 3 cut(s) 149, 934, 948
MalI GATC 3 cut(s) 321, 872, 1349
MboI GATC 3 cut(s) 319, 870, 1347
MfeI CAATTG 1 cut(s) 289
MluCI AATT 7 cut(s) 289, 468, 479, 570, 638, 998, 1161
MlyI GAGTC 5 cut(s) 566, 605, 1120, 1269, 1348
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 7 cut(s) 220, 233, 296, 696, 1048, 1250, 1306
MroI TCCGGA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 888
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 4 cut(s) 26, 57, 66, 1272
MspR9I CCNGG 2 cut(s) 816, 1272
MunI CAATTG 1 cut(s) 289
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 1 cut(s) 816
MvnI CGCG 1 cut(s) 1239
NciI CCSGG 1 cut(s) 1272
NcoI CCATGG 1 cut(s) 313
NdeII GATC 3 cut(s) 319, 870, 1347
NlaIII CATG 3 cut(s) 317, 416, 809
NlaIV GGNNCC 2 cut(s) 525, 1028
NmuCI GTSAC 1 cut(s) 948
PctI GAATGC 1 cut(s) 857
PfeI GAWTC 3 cut(s) 248, 344, 464
PinAI ACCGGT 2 cut(s) 25, 65
PkrI GCNGC 2 cut(s) 187, 1305
PleI GAGTC 5 cut(s) 565, 604, 1119, 1268, 1348
PpsI GAGTC 5 cut(s) 565, 604, 1119, 1268, 1348
PpuMI RGGWCCY 1 cut(s) 1169
PshBI ATTAAT 1 cut(s) 822
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 814
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 2 cut(s) 525, 1028
PspPI GGNCC 7 cut(s) 183, 299, 683, 698, 1040, 1169, 1231
PspPPI RGGWCCY 1 cut(s) 1169
PstNI CAGNNNCTG 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 4 cut(s) 74, 268, 545, 1100
RsaNI GTAC 4 cut(s) 73, 267, 544, 1099
RseI CAYNNNNRTG 1 cut(s) 888
SatI GCNGC 2 cut(s) 186, 1304
Sau3AI GATC 3 cut(s) 319, 870, 1347
Sau96I GGNCC 7 cut(s) 183, 299, 683, 698, 1040, 1169, 1231
SchI GAGTC 5 cut(s) 566, 605, 1120, 1269, 1348
ScrFI CCNGG 2 cut(s) 816, 1272
SfaNI GCATC 2 cut(s) 62, 748
SinI GGWCC 5 cut(s) 299, 683, 698, 1040, 1169
SmiMI CAYNNNNRTG 1 cut(s) 888
SmlI CTYRAG 3 cut(s) 92, 1310, 1356
SmoI CTYRAG 3 cut(s) 92, 1310, 1356
Sse9I AATT 7 cut(s) 289, 468, 479, 570, 638, 998, 1161
SsiI CCGC 5 cut(s) 143, 186, 190, 602, 1215
SspMI CTAG 3 cut(s) 134, 1115, 1247
StyD4I CCNGG 2 cut(s) 814, 1270
StyI CCWWGG 2 cut(s) 313, 1018
TaaI ACNGT 2 cut(s) 111, 303
TaiI ACGT 4 cut(s) 78, 649, 905, 1073
TaqI TCGA 2 cut(s) 177, 869
TasI AATT 7 cut(s) 289, 468, 479, 570, 638, 998, 1161
TatI WGTACW 1 cut(s) 266
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 3 cut(s) 248, 344, 464
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 948
TseI GCWGC 1 cut(s) 1303
Tsp45I GTSAC 1 cut(s) 948
TspDTI ATGAA 3 cut(s) 17, 840, 1075
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 5 cut(s) 299, 683, 698, 1040, 1169
VspI ATTAAT 1 cut(s) 822
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 2 cut(s) 998, 1161
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmiI GTMKAC 1 cut(s) 1263
XspI CTAG 3 cut(s) 134, 1115, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.