Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0002135

Physical Location & Seq

Genomic Coordinates Reverse (-)

65544696 .. 65556732

Gene Structure 12037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0525200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGGCCAACTCAAACTCTCTTCCTCTCTATGAAAGGGTTGAGCACAGAAACACACTGCATAAAATCATGGAGTTTATAATCCTCTTCCTCCTCCTTTCACTCCTTGTTTATCGTCTCCTCTCTTTCAACAACCATGGTTTATCCTGGTCCATCGCATTCCTCTGCGAGCTCTGGTTTACCTTCAGTTGGGTCGTCACCATCAGCAACAAATGGATTCTTGTTGACCATAAACCATACCCCGACCGCCTATTGCAACGGGTGCCTAAGGATGAGCTTCCAGCAGTAGACTTGTTTGTGACAACAGCAGACCCAGAGCTTGAACCACCTATCATTACCGTAAACACTGTTCTGTCTCTGTTGGCAGTTGATTACCCAACACAGAAACTAGCATGCTATGTTTCTGATGATGGGTGTTCCCCTATCACCTTCTACTCACTTGTGGAAGCATCAAAATTTGCTAAGATTTGGATTCCCTTTTGCAAAAAGTACAATGTTCAAGTTAGAGCTCCTTTTAGATACTTCACCAATAATTCCACGCTTTCTGGAAATAATAATAATAGCTCAATGGAATTCAAGCATGAATGGAAGATAGTGAAGGATGAATATGAGCAACTTAGCAGCAAAATAGAGGATGCGGTGCGCAAATCAATCAATCCATCTGATATTATCGAATGCGACTTTTCAGTGTTTTCAAATGTACAGCGTAATGATCATCCAACAATAATCAAGGTTGTATGGGAGAACAAAGAAGCACATTCGAATCGTCCAGTGCCACATTTAGTTTATATATCCAGAGAAAAACGGCCAAAGCACCCACATCATGCCAAAGCCGGGGCCATGAATGTTCTGACTAGAGTCTCTGGATTGATGACCAATGCTCCGTACATGCTAAACGTGGACTGTGACATGTTCGTCAACAGCCCAAACATTATTCTTCATGCAATGTGTATGCTACTTGGATCAAAGAATGAAAATGAAAATGCGTTTGTTCAGTGTCCTCAAGTCTTCTGTGACAGTCTAAAGGATGATCCTTTCGGAAATCAGATGATAGTTTGGTGGAAGTATGTGGGACAAGGAGTTTCAGGAATTCAGGGACCTTTTTATTCCGGATCAGGATGTGTTCATACACGAAAAGTCATCTATGGTCTATTCCCCAATTACGTGAAAGTTGGAGAAACAAATGCGAAAAACTTGATGAACGGTAAATCAGCCGATGAAGAGTTACTTGATGTATTTGGGAAGGAATTTACCAAATCAGCTGCTGATGCTTTAAACGGGAAGGCAGTGTGCCCAGACACGCTTACAGAAACTATTGAGGCTGCATACCAAGTTGCTGGTGCTGGATATGAGTATGGCACACAATGGGGCAAAAAGGTGGGATGGATGTATGGATCCTCAACTGAAGATGCCCTCACTGGGCTTTCAATCCATAACAAAGGTTGGAGATCCATTGTTTATGCACCAGACCCACCAGCCTTTCTAGAATGCGCGCCTATTGGTGGGCCTGCTGTAATGACCCAACAGAAAAGGTGGGCCACCGGCCTGCTTGAAATCCTCCTCAGTAAAAACTGCCCCATATTTGGTACTCTCTTTGCAAACCTCCGGTTCAGGCAGTGCTTGGGCTACTTCAGTATCCTTTCATGGGGGATAAGCTCAGTTCTCGAGCTATACTATGCTGCTCTGCCAGCCTATAGTATCATCACCAACTCACACTTCTTGCCGAAGGGCCAAGAACCAGCTTACTATATACTCATAGCTGTGTTTCTGATCTACACTATGTACACTTCATCCGAGTACCTTAGAACCGGTCAATCAATCCGAGCATGGTGGAATAACCAAAGAATGGGAAGAATAATCTCAATGAGTGCATGGCTTTTTGGATTTCTTAGTGTCATACTCAAACTCTTGGGCATATCCGAGACAGTCTTCGAGGTAACACGAAAAGACCAATCTACATCAAGCGATGAAGGCACTGAAGCCGGAAAATTCACCTTTGACCAGTCCCCAGTTTTCATACCTCCCACAGTAATGTTGTTTCTGCAATTAACAGCACTGGCCACCGCTTTGTTGGGGCTTCAACCACCAGCCCGTGATGGGGTTGAATCAGGCTCTTTGGAGACCATGTGCAGTGTTTTGTTGGTGATATGCTTATTACCATTTTTGAAAGGTCTGTTTGGATCAGGAAAATATGGAATTCCTTTGTCTACCATATTTAAGTCTGCTGGTCTTGCACTACTCTTCTACACCTTGTGCAGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0031090 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sacchrp_dh_NADP

Protein Analysis

753

Amino Acids

84.54

Weight (kDa)

7.18

Isoelectric Point (pI)

36.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cellulose_synt	PF03552	95 - 390	2.2e-85	Cellulose synthase
Cellulose_synt	PF03552	412 - 729	6.3e-52	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39410
fragaria_vesca	FvH4_6g47610 FvH4_6g47650 FvH4_6g47650 FvH4_6g47700
malus_domestica	MD09G1060600.v1.1
prunus_persica	Prupe.3G260300_v2.0.a1
pyrus_communis	pycom111g05100
rosa_chinensis	RchiOBHm_Chr2g0166731 RchiOBHm_Chr2g0166761 RchiOBHm_Chr2g0166771 RchiOBHm_Chr4g0415091
rosa_laevigata	RLG00000021643 RLG00000021646 RLG00000021664 RLG00000021667
rosa_multiflora	Rmu_co8040738.1_g000001 Rmu_sc0000949.1_g000002 Rmu_sc0000949.1_g000005 Rmu_sc0002181.1_g000013 Rmu_sc0002181.1_g000015 Rmu_sc0002181.1_g000024 Rmu_sc0004406.1_g000008
rosa_roxburghii	Rroxscaffold_2G00084230 Rroxscaffold_2G00084260 Rroxscaffold_2G00084280 Rroxscaffold_2G00084550 Rroxscaffold_2G00084590 Rroxscaffold_2G00084600
rosa_rugosa	Rorug02G0525000 Rorug02G0525200
rosa_samantha	Rh2AG591500 Rh2BG603200 Rh2BG603500 Rh2CG356500 Rh2CG574100 Rh2CG574500 Rh2DG614200 Rh2DG614300 Rh4BG125500
rosa_wichuraiana	Rw2G049260 Rw2G049280 Rw2G049300 Rw4G016320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	77
AasI	GACNNNNNNGTC	1 cut(s)	911
Acc16I	TGCGCA	1 cut(s)	641
AccB1I	GGYRCC	1 cut(s)	259
AccB7I	CCANNNNNTGG	1 cut(s)	1843
AccI	GTMKAC	2 cut(s)	285, 2204
AccII	CGCG	1 cut(s)	1490
AccIII	TCCGGA	1 cut(s)	1106
AciI	CCGC	3 cut(s)	244, 635, 2061
AclWI	GGATC	7 cut(s)	967, 1022, 1117, 1386, 1399, 1440, 2185
AcoI	YGGCCR	3 cut(s)	3, 803, 2055
AcsI	RAATTY	6 cut(s)	452, 569, 1086, 1244, 1985, 2193
AcuI	CTGAAG	4 cut(s)	166, 1422, 1612, 1995
AdeI	CACNNNGTG	1 cut(s)	2250
AfaI	GTAC	6 cut(s)	488, 699, 884, 1585, 1781, 1796
AfiI	CCNNNNNNNGG	9 cut(s)	186, 831, 1416, 1499, 1580, 1642, 1843, 2094, 2095
AflIII	ACRYGT	1 cut(s)	906
AgeI	ACCGGT	1 cut(s)	1805
AjnI	CCWGG	1 cut(s)	143
AjuI	GAANNNNNNNTTGG	2 cut(s)	1697, 1729
Alw21I	GWGCWC	3 cut(s)	45, 171, 508
Alw26I	GTCTC	5 cut(s)	119, 357, 862, 1913, 2111
AlwI	GGATC	7 cut(s)	967, 1022, 1117, 1386, 1399, 1440, 2185
AlwNI	CAGNNNCTG	1 cut(s)	1262
Ama87I	CYCGRG	1 cut(s)	1661
Aor13HI	TCCGGA	1 cut(s)	1106
AoxI	GGCC	8 cut(s)	3, 803, 834, 1502, 1533, 1540, 1726, 2055
ApeKI	GCWGC	4 cut(s)	618, 1259, 1319, 1676
ApoI	RAATTY	6 cut(s)	452, 569, 1086, 1244, 1985, 2193
AsiGI	ACCGGT	1 cut(s)	1805
AspLEI	GCGC	3 cut(s)	642, 1490, 1492
AspS9I	GGNCC	6 cut(s)	147, 834, 1094, 1502, 1533, 1726
AsuC2I	CCSGG	1 cut(s)	832
AsuHPI	GGTGA	6 cut(s)	187, 415, 514, 1693, 1981, 2152
AsuII	TTCGAA	1 cut(s)	758
AvaI	CYCGRG	1 cut(s)	1661
AvaII	GGWCC	2 cut(s)	147, 1094
AxyI	CCTNAGG	1 cut(s)	264
BaeGI	GKGCMC	1 cut(s)	1292
BaeI	ACNNNNGTAYC	2 cut(s)	1615, 1648
BalI	TGGCCA	2 cut(s)	5, 2057
BamHI	GGATCC	1 cut(s)	1391
BanI	GGYRCC	1 cut(s)	259
BanII	GRGCYC	2 cut(s)	171, 508
BbsI	GAAGAC	2 cut(s)	997, 1918
Bbv12I	GWGCWC	3 cut(s)	45, 171, 508
BbvI	GCAGC	4 cut(s)	630, 1246, 1306, 1663
BccI	CCATC	6 cut(s)	158, 206, 401, 664, 1374, 2087
BceAI	ACGGC	1 cut(s)	818
BciT130I	CCWGG	1 cut(s)	145
BciVI	GTATCC	1 cut(s)	1643
BclI	TGATCA	1 cut(s)	709
BcnI	CCSGG	1 cut(s)	832
BcoDI	GTCTC	5 cut(s)	119, 357, 862, 1913, 2111
BfaI	CTAG	3 cut(s)	386, 852, 1481
BfmI	CTRYAG	1 cut(s)	1690
BfuI	GTATCC	1 cut(s)	1643
BisI	GCNGC	4 cut(s)	619, 1260, 1320, 1677
BlsI	GCNGC	4 cut(s)	620, 1261, 1321, 1678
Bme1390I	CCNGG	2 cut(s)	145, 832
Bme18I	GGWCC	2 cut(s)	147, 1094
BmeT110I	CYCGRG	1 cut(s)	1661
BmgT120I	GGNCC	6 cut(s)	147, 834, 1094, 1502, 1533, 1726
BmiI	GGNNCC	4 cut(s)	261, 835, 1095, 1393
BmrFI	CCNGG	2 cut(s)	145, 832
BmrI	ACTGGG	2 cut(s)	1425, 2000
BmsI	GCATC	4 cut(s)	455, 622, 1255, 1396
BmuI	ACTGGG	2 cut(s)	1425, 2000
BoxI	GACNNNNGTC	1 cut(s)	854
BpiI	GAAGAC	2 cut(s)	997, 1918
Bpu14I	TTCGAA	1 cut(s)	758
BpuEI	CTTGAG	1 cut(s)	984
BpuMI	CCSGG	1 cut(s)	832
BsaAI	YACGTR	1 cut(s)	1162
BsaI	GGTCTC	1 cut(s)	2111
BsaJI	CCNNGG	2 cut(s)	133, 831
BsaWI	WCCGGW	3 cut(s)	1106, 1602, 1805
BsaXI	ACNNNNNCTCC	4 cut(s)	862, 892, 2108, 2138
Bsc4I	CCNNNNNNNGG	9 cut(s)	186, 831, 1416, 1499, 1580, 1642, 1843, 2094, 2095
Bse118I	RCCGGY	2 cut(s)	1538, 1805
Bse1I	ACTGG	5 cut(s)	767, 1420, 1999, 2006, 2058
Bse21I	CCTNAGG	1 cut(s)	264
Bse3DI	GCAATG	1 cut(s)	948
BseAI	TCCGGA	1 cut(s)	1106
BseBI	CCWGG	1 cut(s)	145
BseDI	CCNNGG	2 cut(s)	133, 831
BseGI	GGATG	9 cut(s)	274, 604, 637, 712, 1030, 1121, 1385, 1389, 1787
BseLI	CCNNNNNNNGG	9 cut(s)	186, 831, 1416, 1499, 1580, 1642, 1843, 2094, 2095
BseMI	GCAATG	1 cut(s)	948
BseMII	CTCAG	2 cut(s)	1573, 1668
BseNI	ACTGG	5 cut(s)	767, 1420, 1999, 2006, 2058
BsePI	GCGCGC	1 cut(s)	1488
BseRI	GAGGAG	3 cut(s)	80, 107, 1547
BseSI	GKGCMC	1 cut(s)	1292
BseXI	GCAGC	4 cut(s)	630, 1246, 1306, 1663
BsgI	GTGCAG	1 cut(s)	2146
Bsh1236I	CGCG	1 cut(s)	1490
Bsh1285I	CGRYCG	1 cut(s)	244
BshFI	GGCC	8 cut(s)	5, 805, 836, 1504, 1535, 1542, 1728, 2057
BshNI	GGYRCC	1 cut(s)	259
BshTI	ACCGGT	1 cut(s)	1805
BsiEI	CGRYCG	1 cut(s)	244
BsiHKAI	GWGCWC	3 cut(s)	45, 171, 508
BsiHKCI	CYCGRG	1 cut(s)	1661
BsiSI	CCGG	6 cut(s)	831, 1107, 1539, 1603, 1806, 1980
BslFI	GGGAC	3 cut(s)	1083, 1107, 1987
BslI	CCNNNNNNNGG	9 cut(s)	186, 831, 1416, 1499, 1580, 1642, 1843, 2094, 2095
BsmAI	GTCTC	5 cut(s)	119, 357, 862, 1913, 2111
BsmBI	CGTCTC	1 cut(s)	119
BsmFI	GGGAC	3 cut(s)	1083, 1107, 1987
BsmI	GAATGC	3 cut(s)	155, 677, 1490
BsnI	GGCC	8 cut(s)	5, 805, 836, 1504, 1535, 1542, 1728, 2057
Bso31I	GGTCTC	1 cut(s)	2111
BsoBI	CYCGRG	1 cut(s)	1661
Bsp119I	TTCGAA	1 cut(s)	758
Bsp1286I	GDGCHC	4 cut(s)	45, 171, 508, 1292
Bsp13I	TCCGGA	1 cut(s)	1106
Bsp1407I	TGTACA	2 cut(s)	697, 1779
Bsp143I	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
Bsp19I	CCATGG	1 cut(s)	133
BspACI	CCGC	3 cut(s)	244, 635, 2061
BspANI	GGCC	8 cut(s)	5, 805, 836, 1504, 1535, 1542, 1728, 2057
BspCNI	CTCAG	2 cut(s)	1572, 1667
BspEI	TCCGGA	1 cut(s)	1106
BspFNI	CGCG	1 cut(s)	1490
BspLI	GGNNCC	4 cut(s)	261, 835, 1095, 1393
BspPI	GGATC	7 cut(s)	967, 1022, 1117, 1386, 1399, 1440, 2185
BspT104I	TTCGAA	1 cut(s)	758
BspT107I	GGYRCC	1 cut(s)	259
BspTNI	GGTCTC	1 cut(s)	2111
BsrDI	GCAATG	1 cut(s)	948
BsrFI	RCCGGY	2 cut(s)	1538, 1805
BsrGI	TGTACA	2 cut(s)	697, 1779
BsrI	ACTGG	5 cut(s)	767, 1420, 1999, 2006, 2058
BssAI	RCCGGY	2 cut(s)	1538, 1805
BssECI	CCNNGG	2 cut(s)	133, 831
BssHII	GCGCGC	1 cut(s)	1488
BssMI	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
BssT1I	CCWWGG	1 cut(s)	133
Bst2UI	CCWGG	1 cut(s)	145
Bst4CI	ACNGT	7 cut(s)	337, 346, 902, 1016, 1202, 1924, 2026
Bst6I	CTCTTC	4 cut(s)	24, 89, 1212, 2243
BstAPI	GCANNNNNTGC	1 cut(s)	259
BstAUI	TGTACA	2 cut(s)	697, 1779
BstBAI	YACGTR	1 cut(s)	1162
BstBI	TTCGAA	1 cut(s)	758
BstC8I	GCNNGC	6 cut(s)	167, 391, 1490, 1506, 1544, 1686
BstDEI	CTNAG	7 cut(s)	264, 459, 614, 1559, 1654, 1799, 1886
BstDSI	CCRYGG	1 cut(s)	133
BstF5I	GGATG	9 cut(s)	274, 604, 637, 712, 1030, 1121, 1385, 1389, 1787
BstFNI	CGCG	1 cut(s)	1490
BstHHI	GCGC	3 cut(s)	642, 1490, 1492
BstKTI	GATC	8 cut(s)	712, 962, 1030, 1112, 1394, 1448, 1770, 2180
BstMAI	GTCTC	5 cut(s)	119, 357, 862, 1913, 2111
BstMBI	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
BstMCI	CGRYCG	1 cut(s)	244
BstMWI	GCNNNNNNNGC	5 cut(s)	259, 1265, 1685, 1968, 2228
BstNI	CCWGG	1 cut(s)	145
BstNSI	RCATGY	3 cut(s)	393, 889, 910
BstPAI	GACNNNNGTC	1 cut(s)	854
BstSCI	CCNGG	2 cut(s)	143, 830
BstSFI	CTRYAG	1 cut(s)	1690
BstSLI	GKGCMC	1 cut(s)	1292
BstUI	CGCG	1 cut(s)	1490
BstV1I	GCAGC	4 cut(s)	630, 1246, 1306, 1663
BstV2I	GAAGAC	2 cut(s)	997, 1918
BstX2I	RGATCY	2 cut(s)	1391, 1445
BstXI	CCANNNNNNTGG	1 cut(s)	1334
BstYI	RGATCY	2 cut(s)	1391, 1445
Bsu36I	CCTNAGG	1 cut(s)	264
BsuI	GTATCC	1 cut(s)	1643
BsuRI	GGCC	8 cut(s)	5, 805, 836, 1504, 1535, 1542, 1728, 2057
BtgI	CCRYGG	1 cut(s)	133
BtgZI	GCGATG	2 cut(s)	136, 1977
BtsCI	GGATG	9 cut(s)	274, 604, 637, 712, 1030, 1121, 1385, 1389, 1787
BtsI	GCAGTG	4 cut(s)	53, 1290, 1619, 2134
Cac8I	GCNNGC	6 cut(s)	167, 391, 1490, 1506, 1544, 1686
CaiI	CAGNNNCTG	1 cut(s)	1262
CfoI	GCGC	3 cut(s)	642, 1490, 1492
Cfr10I	RCCGGY	2 cut(s)	1538, 1805
Cfr13I	GGNCC	6 cut(s)	147, 834, 1094, 1502, 1533, 1726
Csp6I	GTAC	6 cut(s)	487, 698, 883, 1584, 1780, 1795
CspAI	ACCGGT	1 cut(s)	1805
CviQI	GTAC	6 cut(s)	487, 698, 883, 1584, 1780, 1795
DdeI	CTNAG	7 cut(s)	264, 459, 614, 1559, 1654, 1799, 1886
DpnI	GATC	8 cut(s)	711, 961, 1029, 1111, 1393, 1447, 1769, 2179
DpnII	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
DraI	TTTAAA	1 cut(s)	1272
DraIII	CACNNNGTG	1 cut(s)	2250
DrdI	GACNNNNNNGTC	1 cut(s)	911
DseDI	GACNNNNNNGTC	1 cut(s)	911
EaeI	YGGCCR	3 cut(s)	3, 803, 2055
Eam1104I	CTCTTC	4 cut(s)	24, 89, 1212, 2243
EarI	CTCTTC	4 cut(s)	24, 89, 1212, 2243
Ecl136II	GAGCTC	2 cut(s)	169, 506
Eco130I	CCWWGG	1 cut(s)	133
Eco24I	GRGCYC	2 cut(s)	171, 508
Eco31I	GGTCTC	1 cut(s)	2111
Eco47I	GGWCC	2 cut(s)	147, 1094
Eco53kI	GAGCTC	2 cut(s)	169, 506
Eco57I	CTGAAG	4 cut(s)	166, 1422, 1612, 1995
Eco81I	CCTNAGG	1 cut(s)	264
Eco88I	CYCGRG	1 cut(s)	1661
EcoICRI	GAGCTC	2 cut(s)	169, 506
EcoO109I	RGGNCCY	1 cut(s)	1094
EcoRI	GAATTC	3 cut(s)	569, 1086, 2193
EcoRII	CCWGG	1 cut(s)	143
EcoT14I	CCWWGG	1 cut(s)	133
EcoT38I	GRGCYC	2 cut(s)	171, 508
ErhI	CCWWGG	1 cut(s)	133
Esp3I	CGTCTC	1 cut(s)	119
FalI	AAGNNNNNCTT	2 cut(s)	1209, 1241
FaqI	GGGAC	3 cut(s)	1083, 1107, 1987
FbaI	TGATCA	1 cut(s)	709
FblI	GTMKAC	2 cut(s)	285, 2204
Fnu4HI	GCNGC	4 cut(s)	619, 1260, 1320, 1677
FokI	GGATG	9 cut(s)	281, 611, 644, 699, 1037, 1128, 1392, 1396, 1774
FriOI	GRGCYC	2 cut(s)	171, 508
Fsp4HI	GCNGC	4 cut(s)	619, 1260, 1320, 1677
FspBI	CTAG	3 cut(s)	386, 852, 1481
FspI	TGCGCA	1 cut(s)	641
GlaI	GCGC	3 cut(s)	641, 1489, 1491
GluI	GCNGC	4 cut(s)	619, 1260, 1320, 1677
HaeIII	GGCC	8 cut(s)	5, 805, 836, 1504, 1535, 1542, 1728, 2057
HapII	CCGG	6 cut(s)	831, 1107, 1539, 1603, 1806, 1980
HhaI	GCGC	3 cut(s)	642, 1490, 1492
Hin6I	GCGC	3 cut(s)	640, 1488, 1490
HinP1I	GCGC	3 cut(s)	640, 1488, 1490
HincII	GTYRAC	2 cut(s)	223, 916
HindII	GTYRAC	2 cut(s)	223, 916
HinfI	GANTC	5 cut(s)	214, 469, 760, 855, 2102
HpaII	CCGG	6 cut(s)	831, 1107, 1539, 1603, 1806, 1980
HphI	GGTGA	6 cut(s)	187, 415, 514, 1693, 1981, 2152
Hpy166II	GTNNAC	8 cut(s)	177, 223, 286, 340, 898, 916, 1782, 2205
Hpy188I	TCNGA	9 cut(s)	403, 661, 849, 1037, 1044, 1767, 1792, 1820, 1918
Hpy188III	TCNNGA	9 cut(s)	543, 792, 861, 1083, 1107, 1113, 1481, 1661, 2181
Hpy8I	GTNNAC	8 cut(s)	177, 223, 286, 340, 898, 916, 1782, 2205
HpyAV	CCTTC	7 cut(s)	190, 436, 589, 1234, 1273, 1717, 1961
HpyCH4III	ACNGT	7 cut(s)	337, 346, 902, 1016, 1202, 1924, 2026
HpyCH4IV	ACGT	2 cut(s)	894, 1161
HpyF10VI	GCNNNNNNNGC	5 cut(s)	259, 1265, 1685, 1968, 2228
HpyF3I	CTNAG	7 cut(s)	264, 459, 614, 1559, 1654, 1799, 1886
HpySE526I	ACGT	2 cut(s)	894, 1161
HspAI	GCGC	3 cut(s)	640, 1488, 1490
Kpn2I	TCCGGA	1 cut(s)	1106
Ksp22I	TGATCA	1 cut(s)	709
Kzo9I	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
LmnI	GCTCC	2 cut(s)	511, 883
Lsp1109I	GCAGC	4 cut(s)	630, 1246, 1306, 1663
LweI	GCATC	4 cut(s)	455, 622, 1255, 1396
MaeI	CTAG	3 cut(s)	386, 852, 1481
MaeII	ACGT	2 cut(s)	894, 1161
MaeIII	GTNAC	6 cut(s)	193, 295, 902, 1010, 1221, 1933
MalI	GATC	8 cut(s)	711, 961, 1029, 1111, 1393, 1447, 1769, 2179
MboI	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
MflI	RGATCY	2 cut(s)	1391, 1445
MhlI	GDGCHC	4 cut(s)	45, 171, 508, 1292
MlsI	TGGCCA	2 cut(s)	5, 2057
MluCI	AATT	9 cut(s)	452, 529, 569, 1086, 1156, 1244, 1985, 2042, 2193
MluNI	TGGCCA	2 cut(s)	5, 2057
MlyI	GAGTC	1 cut(s)	864
MmeI	TCCRAC	3 cut(s)	740, 1150, 1421
Mox20I	TGGCCA	2 cut(s)	5, 2057
MroI	TCCGGA	1 cut(s)	1106
MscI	TGGCCA	2 cut(s)	5, 2057
MseI	TTAA	3 cut(s)	1271, 2045, 2214
MslI	CAYNNNNRTG	2 cut(s)	1757, 2027
Msp20I	TGGCCA	2 cut(s)	5, 2057
MspA1I	CMGCKG	1 cut(s)	1259
MspI	CCGG	6 cut(s)	831, 1107, 1539, 1603, 1806, 1980
MspR9I	CCNGG	2 cut(s)	145, 832
Mva1269I	GAATGC	3 cut(s)	155, 677, 1490
MvaI	CCWGG	1 cut(s)	145
MvnI	CGCG	1 cut(s)	1490
MwoI	GCNNNNNNNGC	5 cut(s)	259, 1265, 1685, 1968, 2228
NciI	CCSGG	1 cut(s)	832
NcoI	CCATGG	1 cut(s)	133
NdeII	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
NlaIV	GGNNCC	4 cut(s)	261, 835, 1095, 1393
NmuCI	GTSAC	4 cut(s)	193, 295, 902, 1010
NsbI	TGCGCA	1 cut(s)	641
NspI	RCATGY	3 cut(s)	393, 889, 910
NspV	TTCGAA	1 cut(s)	758
PaeI	GCATGC	1 cut(s)	393
PaeR7I	CTCGAG	1 cut(s)	1661
PauI	GCGCGC	1 cut(s)	1488
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	155, 677, 1490
PfeI	GAWTC	4 cut(s)	214, 469, 760, 2102
PflMI	CCANNNNNTGG	1 cut(s)	1843
PinAI	ACCGGT	1 cut(s)	1805
PkrI	GCNGC	4 cut(s)	620, 1261, 1321, 1678
PleI	GAGTC	1 cut(s)	863
PpsI	GAGTC	1 cut(s)	863
Ppu21I	YACGTR	1 cut(s)	1162
PpuMI	RGGWCCY	1 cut(s)	1094
PscI	ACATGT	1 cut(s)	906
PshAI	GACNNNNGTC	1 cut(s)	854
PsiI	TTATAA	1 cut(s)	77
Psp124BI	GAGCTC	2 cut(s)	171, 508
Psp5II	RGGWCCY	1 cut(s)	1094
Psp6I	CCWGG	1 cut(s)	143
PspGI	CCWGG	1 cut(s)	143
PspN4I	GGNNCC	4 cut(s)	261, 835, 1095, 1393
PspPI	GGNCC	6 cut(s)	147, 834, 1094, 1502, 1533, 1726
PspPPI	RGGWCCY	1 cut(s)	1094
PsrI	GAACNNNNNNTAC	2 cut(s)	330, 362
PstNI	CAGNNNCTG	1 cut(s)	1262
PsuI	RGATCY	2 cut(s)	1391, 1445
PteI	GCGCGC	1 cut(s)	1488
PvuII	CAGCTG	1 cut(s)	1259
RsaI	GTAC	6 cut(s)	488, 699, 884, 1585, 1781, 1796
RsaNI	GTAC	6 cut(s)	487, 698, 883, 1584, 1780, 1795
RseI	CAYNNNNRTG	2 cut(s)	1757, 2027
SacI	GAGCTC	2 cut(s)	171, 508
SaqAI	TTAA	3 cut(s)	1271, 2045, 2214
SatI	GCNGC	4 cut(s)	619, 1260, 1320, 1677
Sau3AI	GATC	8 cut(s)	709, 959, 1027, 1109, 1391, 1445, 1767, 2177
Sau96I	GGNCC	6 cut(s)	147, 834, 1094, 1502, 1533, 1726
SchI	GAGTC	1 cut(s)	864
ScrFI	CCNGG	2 cut(s)	145, 832
SduI	GDGCHC	4 cut(s)	45, 171, 508, 1292
SfaNI	GCATC	4 cut(s)	455, 622, 1255, 1396
SfcI	CTRYAG	1 cut(s)	1690
Sfr274I	CTCGAG	1 cut(s)	1661
SfuI	TTCGAA	1 cut(s)	758
SinI	GGWCC	2 cut(s)	147, 1094
SlaI	CTCGAG	1 cut(s)	1661
SmiMI	CAYNNNNRTG	2 cut(s)	1757, 2027
SmlI	CTYRAG	2 cut(s)	999, 1661
SmoI	CTYRAG	2 cut(s)	999, 1661
SphI	GCATGC	1 cut(s)	393
Sse9I	AATT	9 cut(s)	452, 529, 569, 1086, 1156, 1244, 1985, 2042, 2193
SsiI	CCGC	3 cut(s)	244, 635, 2061
SspMI	CTAG	3 cut(s)	386, 852, 1481
SstI	GAGCTC	2 cut(s)	171, 508
StyD4I	CCNGG	2 cut(s)	143, 830
StyI	CCWWGG	1 cut(s)	133
TaaI	ACNGT	7 cut(s)	337, 346, 902, 1016, 1202, 1924, 2026
TaiI	ACGT	2 cut(s)	897, 1164
TaqI	TCGA	4 cut(s)	669, 758, 1662, 1929
TasI	AATT	9 cut(s)	452, 529, 569, 1086, 1156, 1244, 1985, 2042, 2193
TatI	WGTACW	3 cut(s)	486, 697, 1779
TfiI	GAWTC	4 cut(s)	214, 469, 760, 2102
Tru1I	TTAA	3 cut(s)	1271, 2045, 2214
Tru9I	TTAA	3 cut(s)	1271, 2045, 2214
TseFI	GTSAC	4 cut(s)	193, 295, 902, 1010
TseI	GCWGC	4 cut(s)	618, 1259, 1319, 1676
Tsp45I	GTSAC	4 cut(s)	193, 295, 902, 1010
TspGWI	ACGGA	1 cut(s)	870
Van91I	CCANNNNNTGG	1 cut(s)	1843
VpaK11BI	GGWCC	2 cut(s)	147, 1094
XapI	RAATTY	6 cut(s)	452, 569, 1086, 1244, 1985, 2193
XbaI	TCTAGA	1 cut(s)	1480
XceI	RCATGY	3 cut(s)	393, 889, 910
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2065
XhoI	CTCGAG	1 cut(s)	1661
XmiI	GTMKAC	2 cut(s)	285, 2204
XspI	CTAG	3 cut(s)	386, 852, 1481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.