RLG00000021667

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002135
Physical Location & Seq
Reverse (-)
77101604 .. 77102388
785 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021667

Sequence Viewer

Length: 708 bp
ATGTCAGACCTCCCCAACCCTCTCTACGATTTCATCATCCTCGGCGCCTCCGGCTTCACCGGAAAGTACGTCGTGAAAGAAAGCCTCAAGTTCCTCAACACCCCATCTTCACCTCTCAAGTCTCTAGCTTTAGCTGGCCGCAACCCCGCAAAGCTCTCCCAAACTCTCAACTGGGCCACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGATCCTCCGTCCCTCCGCCGCCTCTGCTCCCAGACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAGCCCGTCGTTGCCGCGTGCGTTGAGACTGGCTGTGATTATCTCGACATATGCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAAGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTCGACTCTGTTCCGGCGGAGTTTGGGTTGATGTTTAACTCGAGGCAGTGGGTGGGGGCGGCAGTGCCGAGTAGGGTGGAGGCTTATGTGAGCTTGGAGTCCGAGAAGAGAATTGTTGGCAACTTCGGGACGTTTGAGTCGGCGGTGTTGGGTGTTGCTAATGCGGATAAGTTGATGGAGCTCCGACGGTCCAGACCTAAAAAACCGAGGCCGAAGTTGAAAACTTTGCTGCTATGTTCTGTTTTTGGGTTAGTAATGTAG

Protein Analysis

236

Amino Acids

25.56

Weight (kDa)

8.44

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 144 6e-12 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 323
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 1 cut(s) 68
AgsI TTSAA 1 cut(s) 667
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 5 cut(s) 128, 134, 154, 540, 628
AluI AGCT 5 cut(s) 128, 134, 154, 540, 628
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 2 cut(s) 126, 326
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 487
AoxI GGCC 6 cut(s) 136, 174, 181, 286, 390, 656
ApaI GGGCCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 676
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 5 cut(s) 174, 182, 286, 287, 636
AsuHPI GGTGA 3 cut(s) 49, 102, 205
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 1 cut(s) 636
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 4 cut(s) 290, 312, 425, 630
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 663
BccI CCATC 4 cut(s) 112, 215, 379, 616
BceAI ACGGC 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 126, 326
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 48
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 677
BlsI GCNGC 6 cut(s) 140, 248, 322, 412, 508, 678
Bme18I GGWCC 1 cut(s) 636
BmeT110I CYCGRG 1 cut(s) 487
BmgT120I GGNCC 5 cut(s) 174, 182, 286, 287, 636
BmiI GGNNCC 3 cut(s) 46, 288, 289
BmrI ACTGGG 1 cut(s) 181
BmuI ACTGGG 1 cut(s) 181
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpuEI CTTGAG 2 cut(s) 71, 101
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 40, 653
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 458, 488
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 3 cut(s) 176, 340, 393
BseDI CCNNGG 2 cut(s) 40, 653
BseGI GGATG 4 cut(s) 36, 207, 270, 390
BseNI ACTGG 3 cut(s) 176, 340, 393
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 1 cut(s) 663
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 6 cut(s) 138, 176, 183, 288, 392, 658
BshNI GGYRCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 5 cut(s) 51, 60, 180, 368, 461
BslFI GGGAC 2 cut(s) 223, 589
BsmAI GTCTC 2 cut(s) 126, 326
BsmFI GGGAC 2 cut(s) 223, 589
BsnI GGCC 6 cut(s) 138, 176, 183, 288, 392, 658
BsoBI CYCGRG 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 4 cut(s) 290, 312, 425, 630
Bsp143I GATC 1 cut(s) 229
BspANI GGCC 6 cut(s) 138, 176, 183, 288, 392, 658
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 46, 288, 289
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 3 cut(s) 176, 340, 393
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 40, 653
BssMI GATC 1 cut(s) 229
BssNI GRCGYC 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 636
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 136, 308, 325
BstF5I GGATG 4 cut(s) 36, 207, 270, 390
BstFNI CGCG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 2 cut(s) 126, 326
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 663
BsuRI GGCC 6 cut(s) 138, 176, 183, 288, 392, 658
BtsCI GGATG 4 cut(s) 36, 207, 270, 390
BtsI GCAGTG 2 cut(s) 500, 516
BtsIMutI CAGTG 2 cut(s) 500, 516
Cac8I GCNNGC 3 cut(s) 136, 308, 325
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 5 cut(s) 174, 182, 286, 287, 636
Csp6I GTAC 1 cut(s) 67
CviAII CATG 1 cut(s) 376
CviQI GTAC 1 cut(s) 67
DinI GGCGCC 1 cut(s) 46
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
DrdI GACNNNNNNGTC 1 cut(s) 583
DseDI GACNNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 4 cut(s) 233, 284, 428, 479
Ecl136II GAGCTC 1 cut(s) 628
Eco24I GRGCYC 4 cut(s) 290, 312, 425, 630
Eco47I GGWCC 1 cut(s) 636
Eco53kI GAGCTC 1 cut(s) 628
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 287
EcoT38I GRGCYC 4 cut(s) 290, 312, 425, 630
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 1 cut(s) 379
FaiI YATR 5 cut(s) 356, 358, 377, 534, 682
FaqI GGGAC 2 cut(s) 223, 589
FatI CATG 1 cut(s) 375
FauI CCCGC 2 cut(s) 154, 272
FauNDI CATATG 1 cut(s) 356
Fnu4HI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 677
FokI GGATG 4 cut(s) 23, 194, 257, 397
FriOI GRGCYC 4 cut(s) 290, 312, 425, 630
Fsp4HI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 677
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 46
GluI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 677
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 6 cut(s) 138, 176, 183, 288, 392, 658
HapII CCGG 5 cut(s) 51, 60, 180, 368, 461
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 3 cut(s) 452, 545, 584
HpaII CCGG 5 cut(s) 51, 60, 180, 368, 461
HphI GGTGA 3 cut(s) 49, 102, 205
Hpy188I TCNGA 3 cut(s) 7, 550, 632
Hpy188III TCNNGA 4 cut(s) 73, 350, 574, 639
Hpy99I CGWCG 3 cut(s) 74, 317, 636
HpyAV CCTTC 2 cut(s) 301, 412
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4IV ACGT 2 cut(s) 69, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
HpySE526I ACGT 2 cut(s) 69, 578
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 3 cut(s) 260, 625, 633
Lsp1109I GCAGC 1 cut(s) 663
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 69, 578
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 2 cut(s) 99, 565
MhlI GDGCHC 4 cut(s) 290, 312, 425, 630
MluCI AATT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 3 cut(s) 446, 554, 593
MmeI TCCRAC 1 cut(s) 655
MseI TTAA 1 cut(s) 483
MspI CCGG 5 cut(s) 51, 60, 180, 368, 461
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
NarI GGCGCC 1 cut(s) 45
NdeI CATATG 1 cut(s) 356
NdeII GATC 1 cut(s) 229
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 3 cut(s) 46, 288, 289
NmeAIII GCCGAG 2 cut(s) 21, 540
PaeR7I CTCGAG 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PkrI GCNGC 6 cut(s) 140, 248, 322, 412, 508, 678
PleI GAGTC 3 cut(s) 446, 553, 592
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 3 cut(s) 446, 553, 592
Psp124BI GAGCTC 1 cut(s) 630
PspN4I GGNNCC 3 cut(s) 46, 288, 289
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 5 cut(s) 174, 182, 286, 287, 636
PspXI VCTCGAGB 1 cut(s) 487
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 677
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 5 cut(s) 174, 182, 286, 287, 636
SchI GAGTC 3 cut(s) 446, 554, 593
SduI GDGCHC 4 cut(s) 290, 312, 425, 630
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 487
SinI GGWCC 1 cut(s) 636
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 3 cut(s) 86, 116, 487
SmoI CTYRAG 3 cut(s) 86, 116, 487
Sse9I AATT 1 cut(s) 558
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 630
TaaI ACNGT 1 cut(s) 636
TaiI ACGT 2 cut(s) 72, 581
TaqI TCGA 4 cut(s) 351, 402, 450, 488
TasI AATT 1 cut(s) 558
TauI GCSGC 5 cut(s) 141, 249, 323, 413, 509
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 2 cut(s) 500, 516
TseI GCWGC 1 cut(s) 676
TspDTI ATGAA 2 cut(s) 22, 364
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 2 cut(s) 500, 516
VpaK11BI GGWCC 1 cut(s) 636
XhoI CTCGAG 1 cut(s) 487
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.