Rw2G049280

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002135
Physical Location & Seq
Reverse (-)
76790694 .. 76793104
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049280.1

Sequence Viewer

Length: 1356 bp
ATGTCAGAACTCCCCAACCCTCTCTATGATTTCATCATCCTCGGCGCCTCCGGCTTCACTGGAAAGTACGTCGTCAAAGAAGCCCTCAAGTTCCTCAACACCCCATCTTCACCTCTCAAGTCTCTAGCTTTAGCCGGCCGCAACCCCGCAAAGCTCTCCCAAACTCTCAACTGGGCCACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGACCCTCCGTCCCTCCGCCGCCTCTGCTCCCAAACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAGCCCGTCGTCGCTGCGTGCGTCGAGACCGGCTGTGATTATCTCGACATATCCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAGGCGGCGGAGAAGGGCTCGTTGATCATTTCGGCTTGTGGGTTCGACTCTGTTCCAGCAGAGTTTGGGTTGATGTTTAACTCGAGGCAGTGGGTGGGGGCGGCGGTACCGAATAGGGTGGAGGCTTATGTGAGCTTGGAGTCTGAGAAGAGACTTGTTTTGAACTTCGGGACGTTTGAGTCGGCGGTTTTGGGTGTGGCTAATGCGGATAAGTTGATGGAGCTCCGGCGGTCCAGACCTAAAAAGCCGAGGCCGGAGATTCCTGGTCCTCCTCCTTCTAAAGGACCAACTATTGAACACCAGAAAAAGATTGGCCTTTGGGCTGTGAAGCTACCTACAGCAGATACTATCGCTGTGCGAAGAACACTTGGAGCTCTGACTGAAAATCCCGGTGGTCTTCCAGGGAGGAATGAGAGTGGTGAACACATTAAAAAAAGAGCAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTCGGGGTGAAGATAGGCACCAAGTCTCTGTTGGGCATTTTTCGGATGATGATAATTGGAATCTTTCTTGGGCTCTTGGGTCAGTTTTCCTTTGGGAGGTGGCTTCTTTTAAAGTATCCCCCATTTTTCAGTGTTGGATGGTTCAGGAAGCAGGGTCCCTCCGATGAGGAGGTGAGAAGTGCATCTTTTAAGATGTGGTTTGTTGGATGTGGTTTCAGCGACAGGAGTCTTGCTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAGTTGGCTACATAACAACCCCGATAATTCTACTCCAGTGTGCACTTACTGTTCTGAGCCAACGCGACAATCTTCCAAAGGGAGGAGTTCTCCCACCTGGGATTGTATTTGGCCCAACTGATCTCCAAGAAAAGCTACAAGAAAATGGAATTACTTTTGATGTTATTTCAAAGAATGTTATTTCTCATTAA

Protein Analysis

451

Amino Acids

49.25

Weight (kDa)

9.19

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 144 1.2e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 583
Acc65I GGTACC 1 cut(s) 511
AccB1I GGYRCC 3 cut(s) 44, 511, 893
AccII CGCG 1 cut(s) 1230
AccIII TCCGGA 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 68, 513
AfiI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
AgsI TTSAA 3 cut(s) 568, 701, 1335
AhdI GACNNNNNGTC 1 cut(s) 235
AjnI CCWGG 3 cut(s) 667, 805, 1261
AluBI AGCT 8 cut(s) 128, 154, 540, 628, 736, 779, 872, 1300
AluI AGCT 8 cut(s) 128, 154, 540, 628, 736, 779, 872, 1300
Alw21I GWGCWC 3 cut(s) 630, 781, 1210
Alw26I GTCTC 4 cut(s) 126, 326, 550, 906
Alw44I GTGCAC 1 cut(s) 1206
Ama87I CYCGRG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 8 cut(s) 136, 174, 181, 286, 390, 656, 718, 1276
ApaI GGGCCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 1206
ApeKI GCWGC 2 cut(s) 320, 845
Asp718I GGTACC 1 cut(s) 511
AspLEI GCGC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 5 cut(s) 102, 205, 836, 895, 1060
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 5 cut(s) 636, 671, 689, 1031, 1160
BaeGI GKGCMC 2 cut(s) 290, 1210
BanI GGYRCC 3 cut(s) 44, 511, 893
BanII GRGCYC 6 cut(s) 290, 312, 425, 630, 781, 951
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 3 cut(s) 630, 781, 1210
BbvI GCAGC 2 cut(s) 307, 857
BccI CCATC 5 cut(s) 112, 215, 379, 616, 1008
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 3 cut(s) 669, 807, 1263
BciVI GTATCC 1 cut(s) 1002
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 4 cut(s) 126, 326, 550, 906
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 741
BfoI RGCGCY 1 cut(s) 48
BfuI GTATCC 1 cut(s) 1002
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 846
BlsI GCNGC 6 cut(s) 140, 248, 322, 412, 508, 847
Bme1390I CCNGG 4 cut(s) 669, 795, 807, 1263
Bme18I GGWCC 5 cut(s) 636, 671, 689, 1031, 1160
BmeRI GACNNNNNGTC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 487
BmiI GGNNCC 8 cut(s) 46, 288, 289, 513, 895, 1032, 1033, 1161
BmrFI CCNGG 4 cut(s) 669, 795, 807, 1263
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 1067
BmuI ACTGGG 1 cut(s) 181
BoxI GACNNNNGTC 1 cut(s) 1101
BpiI GAAGAC 1 cut(s) 794
BplI GAGNNNNNCTC 4 cut(s) 407, 439, 1239, 1271
BpmI CTGGAG 1 cut(s) 1184
BpuEI CTTGAG 2 cut(s) 71, 101
BpuMI CCSGG 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 4 cut(s) 40, 653, 806, 1262
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
Bse118I RCCGGY 3 cut(s) 134, 179, 335
Bse1I ACTGG 4 cut(s) 64, 176, 393, 1201
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 3 cut(s) 669, 807, 1263
BseDI CCNNGG 4 cut(s) 40, 653, 806, 1262
BseGI GGATG 7 cut(s) 36, 207, 270, 390, 927, 1019, 1088
BseLI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
BseMII CTCAG 3 cut(s) 540, 1127, 1211
BseNI ACTGG 4 cut(s) 64, 176, 393, 1201
BseRI GAGGAG 3 cut(s) 666, 1058, 1263
BseSI GKGCMC 2 cut(s) 290, 1210
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 307, 857
Bsh1236I CGCG 1 cut(s) 1230
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BshNI GGYRCC 3 cut(s) 44, 511, 893
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 3 cut(s) 630, 781, 1210
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 9 cut(s) 51, 135, 180, 336, 360, 368, 631, 659, 795
BslFI GGGAC 4 cut(s) 223, 589, 1017, 1173
BslI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
BsmAI GTCTC 4 cut(s) 126, 326, 550, 906
BsmFI GGGAC 4 cut(s) 223, 589, 1017, 1173
BsnI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 7 cut(s) 290, 312, 425, 630, 781, 951, 1210
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 2 cut(s) 429, 1285
BspANI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BspCNI CTCAG 3 cut(s) 541, 1126, 1212
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 1230
BspLI GGNNCC 8 cut(s) 46, 288, 289, 513, 895, 1032, 1033, 1161
BspT107I GGYRCC 3 cut(s) 44, 511, 893
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 3 cut(s) 134, 179, 335
BsrI ACTGG 4 cut(s) 64, 176, 393, 1201
BssAI RCCGGY 3 cut(s) 134, 179, 335
BssECI CCNNGG 4 cut(s) 40, 653, 806, 1262
BssMI GATC 2 cut(s) 429, 1285
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 669, 807, 1263
Bst4CI ACNGT 2 cut(s) 862, 1216
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 4 cut(s) 136, 308, 325, 870
BstDEI CTNAG 3 cut(s) 549, 1113, 1220
BstENI CCTNNNNNAGG 2 cut(s) 684, 970
BstF5I GGATG 7 cut(s) 36, 207, 270, 390, 927, 1019, 1088
BstFNI CGCG 1 cut(s) 1230
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 2 cut(s) 432, 1288
BstMAI GTCTC 4 cut(s) 126, 326, 550, 906
BstMBI GATC 2 cut(s) 429, 1285
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
BstNI CCWGG 3 cut(s) 669, 807, 1263
BstPAI GACNNNNGTC 1 cut(s) 1101
BstSCI CCNGG 4 cut(s) 667, 793, 805, 1261
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 2 cut(s) 290, 1210
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 2 cut(s) 307, 857
BstV2I GAAGAC 1 cut(s) 794
BstZI CGGCCG 1 cut(s) 136
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BtsCI GGATG 7 cut(s) 36, 207, 270, 390, 927, 1019, 1088
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 5 cut(s) 57, 500, 867, 1012, 1208
Cac8I GCNNGC 4 cut(s) 136, 308, 325, 870
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 3 cut(s) 134, 179, 335
CseI GACGC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 67, 512
CviAII CATG 1 cut(s) 376
CviQI GTAC 2 cut(s) 67, 512
DdeI CTNAG 3 cut(s) 549, 1113, 1220
DinI GGCGCC 1 cut(s) 46
DpnI GATC 2 cut(s) 431, 1287
DpnII GATC 2 cut(s) 429, 1285
DraI TTTAAA 1 cut(s) 987
DrdI GACNNNNNNGTC 1 cut(s) 583
DriI GACNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 3 cut(s) 233, 284, 428
Ecl136II GAGCTC 2 cut(s) 628, 779
EclXI CGGCCG 1 cut(s) 136
Eco24I GRGCYC 6 cut(s) 290, 312, 425, 630, 781, 951
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 5 cut(s) 636, 671, 689, 1031, 1160
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 2 cut(s) 628, 779
Eco57I CTGAAG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 2 cut(s) 628, 779
EcoNI CCTNNNNNAGG 2 cut(s) 684, 970
EcoO109I RGGNCCY 3 cut(s) 287, 1031, 1160
EcoRII CCWGG 3 cut(s) 667, 805, 1261
EcoT38I GRGCYC 6 cut(s) 290, 312, 425, 630, 781, 951
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 1 cut(s) 379
FaiI YATR 7 cut(s) 27, 356, 377, 534, 1136, 1157, 1178
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 4 cut(s) 223, 589, 1017, 1173
FatI CATG 1 cut(s) 375
FauI CCCGC 2 cut(s) 154, 272
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 846
FokI GGATG 7 cut(s) 23, 194, 257, 397, 934, 1026, 1095
FriOI GRGCYC 6 cut(s) 290, 312, 425, 630, 781, 951
Fsp4HI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 846
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 46
GluI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 846
GsuI CTGGAG 1 cut(s) 1184
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
HapII CCGG 9 cut(s) 51, 135, 180, 336, 360, 368, 631, 659, 795
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 6 cut(s) 452, 545, 584, 664, 936, 1102
HpaII CCGG 9 cut(s) 51, 135, 180, 336, 360, 368, 631, 659, 795
HphI GGTGA 5 cut(s) 102, 205, 836, 895, 1060
Hpy166II GTNNAC 3 cut(s) 827, 858, 1208
Hpy188I TCNGA 6 cut(s) 7, 550, 783, 921, 1039, 1221
Hpy188III TCNNGA 6 cut(s) 331, 350, 360, 574, 639, 1021
Hpy8I GTNNAC 3 cut(s) 827, 858, 1208
Hpy99I CGWCG 4 cut(s) 74, 317, 320, 332
HpyAV CCTTC 4 cut(s) 301, 412, 690, 859
HpyCH4III ACNGT 2 cut(s) 862, 1216
HpyCH4IV ACGT 2 cut(s) 69, 578
HpyCH4V TGCA 2 cut(s) 1058, 1208
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
HpyF3I CTNAG 3 cut(s) 549, 1113, 1220
HpySE526I ACGT 2 cut(s) 69, 578
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KflI GGGWCCC 1 cut(s) 1031
Kpn2I TCCGGA 1 cut(s) 359
KpnI GGTACC 1 cut(s) 515
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 2 cut(s) 429, 1285
LmnI GCTCC 4 cut(s) 260, 625, 633, 776
Lsp1109I GCAGC 2 cut(s) 307, 857
LweI GCATC 1 cut(s) 1067
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 69, 578
MaeIII GTNAC 1 cut(s) 1144
MalI GATC 2 cut(s) 431, 1287
MboI GATC 2 cut(s) 429, 1285
MboII GAAGA 6 cut(s) 99, 565, 777, 794, 898, 1229
MhlI GDGCHC 7 cut(s) 290, 312, 425, 630, 781, 951, 1210
MluCI AATT 3 cut(s) 930, 1191, 1314
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 4 cut(s) 446, 554, 593, 1111
MmeI TCCRAC 2 cut(s) 991, 1060
MroI TCCGGA 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 134
MseI TTAA 5 cut(s) 483, 834, 986, 1065, 1354
MspI CCGG 9 cut(s) 51, 135, 180, 336, 360, 368, 631, 659, 795
MspR9I CCNGG 4 cut(s) 669, 795, 807, 1263
MvaI CCWGG 3 cut(s) 669, 807, 1263
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
NaeI GCCGGC 1 cut(s) 136
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 795
NdeII GATC 2 cut(s) 429, 1285
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 8 cut(s) 46, 288, 289, 513, 895, 1032, 1033, 1161
NmeAIII GCCGAG 2 cut(s) 21, 678
PaeR7I CTCGAG 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 324
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 664, 936
PkrI GCNGC 6 cut(s) 140, 248, 322, 412, 508, 847
PleI GAGTC 4 cut(s) 446, 553, 592, 1110
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 4 cut(s) 446, 553, 592, 1110
PpuMI RGGWCCY 2 cut(s) 1031, 1160
PshAI GACNNNNGTC 1 cut(s) 1101
Psp124BI GAGCTC 2 cut(s) 630, 781
Psp5II RGGWCCY 2 cut(s) 1031, 1160
Psp6I CCWGG 3 cut(s) 667, 805, 1261
PspGI CCWGG 3 cut(s) 667, 805, 1261
PspN4I GGNNCC 8 cut(s) 46, 288, 289, 513, 895, 1032, 1033, 1161
PspOMI GGGCCC 1 cut(s) 286
PspPPI RGGWCCY 2 cut(s) 1031, 1160
PspXI VCTCGAGB 1 cut(s) 487
RsaI GTAC 2 cut(s) 68, 513
RsaNI GTAC 2 cut(s) 67, 512
SacI GAGCTC 2 cut(s) 630, 781
SaqAI TTAA 5 cut(s) 483, 834, 986, 1065, 1354
SatI GCNGC 6 cut(s) 139, 247, 321, 411, 507, 846
Sau3AI GATC 2 cut(s) 429, 1285
SchI GAGTC 4 cut(s) 446, 554, 593, 1111
ScrFI CCNGG 4 cut(s) 669, 795, 807, 1263
SduI GDGCHC 7 cut(s) 290, 312, 425, 630, 781, 951, 1210
SfaNI GCATC 1 cut(s) 1067
SfcI CTRYAG 1 cut(s) 741
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 487
SinI GGWCC 5 cut(s) 636, 671, 689, 1031, 1160
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 3 cut(s) 86, 116, 487
SmoI CTYRAG 3 cut(s) 86, 116, 487
Sse9I AATT 3 cut(s) 930, 1191, 1314
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 2 cut(s) 630, 781
StyD4I CCNGG 4 cut(s) 667, 793, 805, 1261
TaaI ACNGT 2 cut(s) 862, 1216
TaiI ACGT 2 cut(s) 72, 581
TaqI TCGA 5 cut(s) 330, 351, 402, 450, 488
TasI AATT 3 cut(s) 930, 1191, 1314
TauI GCSGC 4 cut(s) 141, 249, 413, 509
TfiI GAWTC 2 cut(s) 664, 936
Tru1I TTAA 5 cut(s) 483, 834, 986, 1065, 1354
Tru9I TTAA 5 cut(s) 483, 834, 986, 1065, 1354
TscAI CASTG 5 cut(s) 64, 500, 867, 1012, 1208
TseI GCWGC 2 cut(s) 320, 845
TspDTI ATGAA 2 cut(s) 22, 364
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 5 cut(s) 64, 500, 867, 1012, 1208
VneI GTGCAC 1 cut(s) 1206
VpaK11BI GGWCC 5 cut(s) 636, 671, 689, 1031, 1160
XagI CCTNNNNNAGG 2 cut(s) 684, 970
XcmI CCANNNNNNNNNTGG 2 cut(s) 713, 904
XhoI CTCGAG 1 cut(s) 487
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.