Rroxscaffold_2G00084280

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002135
Physical Location & Seq
Forward (+)
6797404 .. 6798758
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084280.1

Sequence Viewer

Length: 927 bp
ATGTCTGATCTTCCCAACCCTTTCTACGATCTCATCATCCTCGGCGCCTCCGGCTTCACTGGAAAGTACGTCGTCAAAGAAGCCCTCAAGTTCCTCAACACCCCCTCCTCACCTCTCAAGTCTCTAGCCTTAGCCGGCCGCAACCCCGCGAAGCTCTCCCAAACCCTCAACTGGGCCACCGCCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGATCCCCCGTCCCTCCGCCGCCTCTGCTCCCAAACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGACCCCGTTGTCGCCGCATGCGTGGAAACCGGCTGTGATTATCTCGACATATCCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAGGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTTGACTCTGTTCCGGCGGAGTTTGGATTGATGTTCAACTCGAGGCAGTGGGTGGGGGCGGCGGTGCCGAATAGGGTGGAGGCTTATCTGAGCTTGGAGTCTGATAAGAGAATTGTTGGGAACTTCGGGACGTTTGAGTCGGCGGTGTTGGGTGTGGCTAATGCGGATAAGTTGATGGAGCTCCGGCGGTCCAGGCCTAAAAAACCGAGGCCGGAGATTCCTGGTCCTCCTCCTTCTAAAGGACCAACCATTGAACACCAGAAAAAGATTGGCCTTTGGGCTGTGAAGCTACCTTCAGCAGATGCTATCGTTGTACGAAGAACACTTGGAGCTCTGACTGAAAATCCCGGTGGTCTTCCAGGGAGGAATGAGAGTGATGAACACATTAAAAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTCGGGGTGAAGATAGGCACCAAGTCTCTGTTGGGCATTTTCGAATCATGA

Protein Analysis

308

Amino Acids

33.38

Weight (kDa)

8.88

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 144 1.7e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 314, 583
AccB1I GGYRCC 3 cut(s) 44, 511, 893
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 359
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 726
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 68, 762
AfiI CCNNNNNNNGG 3 cut(s) 171, 172, 686
AgsI TTSAA 2 cut(s) 484, 701
AjnI CCWGG 3 cut(s) 638, 667, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 6 cut(s) 154, 540, 628, 736, 779, 872
AluI AGCT 6 cut(s) 154, 540, 628, 736, 779, 872
Alw21I GWGCWC 2 cut(s) 630, 781
Alw26I GTCTC 2 cut(s) 126, 906
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 7 cut(s) 136, 174, 286, 390, 641, 656, 718
ApaI GGGCCC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 6 cut(s) 174, 286, 287, 636, 671, 689
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 3 cut(s) 102, 205, 895
AsuII TTCGAA 1 cut(s) 918
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 3 cut(s) 636, 671, 689
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 3 cut(s) 44, 511, 893
BanII GRGCYC 4 cut(s) 290, 425, 630, 781
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 2 cut(s) 630, 781
BccI CCATC 3 cut(s) 215, 379, 616
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 3 cut(s) 640, 669, 807
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 2 cut(s) 126, 906
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 48
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 5 cut(s) 139, 247, 321, 411, 507
BlsI GCNGC 5 cut(s) 140, 248, 322, 412, 508
Bme1390I CCNGG 4 cut(s) 640, 669, 795, 807
Bme18I GGWCC 3 cut(s) 636, 671, 689
BmeT110I CYCGRG 1 cut(s) 487
BmgT120I GGNCC 6 cut(s) 174, 286, 287, 636, 671, 689
BmiI GGNNCC 5 cut(s) 46, 288, 289, 513, 895
BmrFI CCNGG 4 cut(s) 640, 669, 795, 807
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 739
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 794
BplI GAGNNNNNCTC 2 cut(s) 407, 439
Bpu10I CCTNAGC 1 cut(s) 130
Bpu14I TTCGAA 1 cut(s) 918
BpuEI CTTGAG 2 cut(s) 71, 101
BpuMI CCSGG 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 40, 653, 806
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 172, 686
Bse118I RCCGGY 2 cut(s) 134, 335
Bse1I ACTGG 3 cut(s) 64, 176, 393
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 3 cut(s) 640, 669, 807
BseDI CCNNGG 3 cut(s) 40, 653, 806
BseGI GGATG 4 cut(s) 36, 207, 270, 390
BseLI CCNNNNNNNGG 3 cut(s) 171, 172, 686
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 3 cut(s) 64, 176, 393
BseRI GAGGAG 2 cut(s) 97, 666
BseSI GKGCMC 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 138, 176, 288, 392, 643, 658, 720
BshNI GGYRCC 3 cut(s) 44, 511, 893
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 630, 781
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 9 cut(s) 51, 135, 336, 360, 368, 461, 631, 659, 795
BslFI GGGAC 2 cut(s) 223, 589
BslI CCNNNNNNNGG 3 cut(s) 171, 172, 686
BsmAI GTCTC 2 cut(s) 126, 906
BsmFI GGGAC 2 cut(s) 223, 589
BsnI GGCC 7 cut(s) 138, 176, 288, 392, 643, 658, 720
BsoBI CYCGRG 1 cut(s) 487
Bsp119I TTCGAA 1 cut(s) 918
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 4 cut(s) 290, 425, 630, 781
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 3 cut(s) 7, 28, 229
BspANI GGCC 7 cut(s) 138, 176, 288, 392, 643, 658, 720
BspCNI CTCAG 1 cut(s) 528
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 149
BspHI TCATGA 1 cut(s) 923
BspLI GGNNCC 5 cut(s) 46, 288, 289, 513, 895
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 918
BspT107I GGYRCC 3 cut(s) 44, 511, 893
BsrFI RCCGGY 2 cut(s) 134, 335
BsrI ACTGG 3 cut(s) 64, 176, 393
BssAI RCCGGY 2 cut(s) 134, 335
BssECI CCNNGG 3 cut(s) 40, 653, 806
BssMI GATC 3 cut(s) 7, 28, 229
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 640, 669, 807
Bst4CI ACNGT 1 cut(s) 862
BstACI GRCGYC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 918
BstC8I GCNNGC 3 cut(s) 136, 325, 870
BstDEI CTNAG 2 cut(s) 130, 536
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 4 cut(s) 36, 207, 270, 390
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 3 cut(s) 10, 31, 232
BstMAI GTCTC 2 cut(s) 126, 906
BstMBI GATC 3 cut(s) 7, 28, 229
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 5 cut(s) 51, 252, 294, 303, 640
BstNI CCWGG 3 cut(s) 640, 669, 807
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 4 cut(s) 638, 667, 793, 805
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 149
BstV2I GAAGAC 1 cut(s) 794
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 7 cut(s) 138, 176, 288, 392, 643, 658, 720
BtsCI GGATG 4 cut(s) 36, 207, 270, 390
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 3 cut(s) 57, 500, 867
Cac8I GCNNGC 3 cut(s) 136, 325, 870
CciI TCATGA 1 cut(s) 923
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 2 cut(s) 134, 335
Cfr13I GGNCC 6 cut(s) 174, 286, 287, 636, 671, 689
Csp6I GTAC 2 cut(s) 67, 761
CviAII CATG 3 cut(s) 324, 376, 924
CviQI GTAC 2 cut(s) 67, 761
DdeI CTNAG 2 cut(s) 130, 536
DinI GGCGCC 1 cut(s) 46
DpnI GATC 3 cut(s) 9, 30, 231
DpnII GATC 3 cut(s) 7, 28, 229
DrdI GACNNNNNNGTC 2 cut(s) 314, 583
DseDI GACNNNNNNGTC 2 cut(s) 314, 583
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
EciI GGCGGA 4 cut(s) 233, 284, 428, 479
Ecl136II GAGCTC 2 cut(s) 628, 779
EclXI CGGCCG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 643
Eco24I GRGCYC 4 cut(s) 290, 425, 630, 781
Eco47I GGWCC 3 cut(s) 636, 671, 689
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 2 cut(s) 628, 779
Eco57I CTGAAG 1 cut(s) 726
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 2 cut(s) 628, 779
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 3 cut(s) 638, 667, 805
EcoT38I GRGCYC 4 cut(s) 290, 425, 630, 781
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 3 cut(s) 327, 379, 927
FaiI YATR 4 cut(s) 325, 356, 377, 925
FaqI GGGAC 2 cut(s) 223, 589
FatI CATG 3 cut(s) 323, 375, 923
FauI CCCGC 2 cut(s) 154, 272
Fnu4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
FokI GGATG 4 cut(s) 23, 194, 257, 397
FriOI GRGCYC 4 cut(s) 290, 425, 630, 781
Fsp4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 139, 247, 321, 411, 507
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 7 cut(s) 138, 176, 288, 392, 643, 658, 720
HapII CCGG 9 cut(s) 51, 135, 336, 360, 368, 461, 631, 659, 795
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 327, 379, 927
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 5 cut(s) 452, 545, 584, 664, 920
HpaII CCGG 9 cut(s) 51, 135, 336, 360, 368, 461, 631, 659, 795
HphI GGTGA 3 cut(s) 102, 205, 895
Hpy166II GTNNAC 1 cut(s) 858
Hpy188I TCNGA 4 cut(s) 7, 537, 550, 783
Hpy188III TCNNGA 4 cut(s) 350, 360, 574, 924
Hpy8I GTNNAC 1 cut(s) 858
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 5 cut(s) 301, 412, 690, 750, 859
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 2 cut(s) 69, 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 252, 294, 303, 640
HpyF3I CTNAG 2 cut(s) 130, 536
HpySE526I ACGT 2 cut(s) 69, 578
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 327, 379, 927
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
Kpn2I TCCGGA 1 cut(s) 359
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 3 cut(s) 7, 28, 229
LmnI GCTCC 4 cut(s) 260, 625, 633, 776
LweI GCATC 1 cut(s) 739
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 69, 578
MalI GATC 3 cut(s) 9, 30, 231
MboI GATC 3 cut(s) 7, 28, 229
MboII GAAGA 3 cut(s) 777, 794, 898
MhlI GDGCHC 4 cut(s) 290, 425, 630, 781
MluCI AATT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 3 cut(s) 446, 554, 593
MroI TCCGGA 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 134
MseI TTAA 1 cut(s) 834
MspI CCGG 9 cut(s) 51, 135, 336, 360, 368, 461, 631, 659, 795
MspR9I CCNGG 4 cut(s) 640, 669, 795, 807
MvaI CCWGG 3 cut(s) 640, 669, 807
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 5 cut(s) 51, 252, 294, 303, 640
NaeI GCCGGC 1 cut(s) 136
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 795
NdeII GATC 3 cut(s) 7, 28, 229
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 3 cut(s) 327, 379, 927
NlaIV GGNNCC 5 cut(s) 46, 288, 289, 513, 895
NmeAIII GCCGAG 1 cut(s) 21
NspI RCATGY 1 cut(s) 327
NspV TTCGAA 1 cut(s) 918
PaeI GCATGC 1 cut(s) 327
PaeR7I CTCGAG 1 cut(s) 487
PagI TCATGA 1 cut(s) 923
PceI AGGCCT 1 cut(s) 643
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 324
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 664, 920
PkrI GCNGC 5 cut(s) 140, 248, 322, 412, 508
PleI GAGTC 3 cut(s) 446, 553, 592
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 3 cut(s) 446, 553, 592
Psp124BI GAGCTC 2 cut(s) 630, 781
Psp6I CCWGG 3 cut(s) 638, 667, 805
PspGI CCWGG 3 cut(s) 638, 667, 805
PspN4I GGNNCC 5 cut(s) 46, 288, 289, 513, 895
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 6 cut(s) 174, 286, 287, 636, 671, 689
PspXI VCTCGAGB 1 cut(s) 487
RsaI GTAC 2 cut(s) 68, 762
RsaNI GTAC 2 cut(s) 67, 761
SacI GAGCTC 2 cut(s) 630, 781
SaqAI TTAA 1 cut(s) 834
SatI GCNGC 5 cut(s) 139, 247, 321, 411, 507
Sau3AI GATC 3 cut(s) 7, 28, 229
Sau96I GGNCC 6 cut(s) 174, 286, 287, 636, 671, 689
SchI GAGTC 3 cut(s) 446, 554, 593
ScrFI CCNGG 4 cut(s) 640, 669, 795, 807
SduI GDGCHC 4 cut(s) 290, 425, 630, 781
SfaNI GCATC 1 cut(s) 739
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 918
SinI GGWCC 3 cut(s) 636, 671, 689
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 3 cut(s) 86, 116, 487
SmoI CTYRAG 3 cut(s) 86, 116, 487
SphI GCATGC 1 cut(s) 327
Sse9I AATT 1 cut(s) 558
SseBI AGGCCT 1 cut(s) 643
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 2 cut(s) 630, 781
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 4 cut(s) 638, 667, 793, 805
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 2 cut(s) 72, 581
TaqI TCGA 4 cut(s) 351, 402, 488, 918
TasI AATT 1 cut(s) 558
TauI GCSGC 5 cut(s) 141, 249, 323, 413, 509
TfiI GAWTC 2 cut(s) 664, 920
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 3 cut(s) 64, 500, 867
TspDTI ATGAA 2 cut(s) 364, 840
TspRI CASTG 3 cut(s) 64, 500, 867
VpaK11BI GGWCC 3 cut(s) 636, 671, 689
XagI CCTNNNNNAGG 1 cut(s) 684
XceI RCATGY 1 cut(s) 327
XcmI CCANNNNNNNNNTGG 2 cut(s) 713, 904
XhoI CTCGAG 1 cut(s) 487
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.