FvH4_6g47610

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002135
Physical Location & Seq
Reverse (-)
36028390 .. 36032223
3834 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47610.t1

Sequence Viewer

Length: 1362 bp
ATGACGTCAGACCTCCCCACTCCTCCCTACGACCTCATCATCCTCGGCGCCTCCGGCTTCACCGGCAAGTACGTCGTCAAAGAAGCCCTCAAGTTCCTCAACACTCCCTCCTCACCTCTCACCTCTCTCGCCTTAGCCGGCCGCAACCCCACCAAGCTCTCTCAAACCCTCACATGGGCCGCCGCCCCAAACCCTCCACCGCCCTCCGTTCCCATCCTCACCGCCGACACCTCCGACCCTCAGTCCCTCCGCCGCCTCTGCTCCCAAACCCACCTCGTCCTCAACTGCGTGGGACCCTTCCGCCTCTACGGCGAGCCCGTCGTCGCTGCATGCGTGGAAAGCGGCTGCGACTATCTCGACATATGCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCGAGTTACTTTGATAAGGCGGCGGAGAAGGGTAGTTTGGTCATTTCGGCATGCGGGTTTGACTCTGTTCCGGCGGAGCTTGGAGTGATGTTTAACTCGAGGCAGTGGGAGGGGGCGGCGGTACCGAATAGGGTGGAGGCTTATTTGAGCTTGGAGAGTGATAAGAGGATTGCGGGGAACTTTGGGACGTTTGAGTCGGCGGTGTTGGGTGTGGCCAATGCGGATAAGTTGATGGAGCTCCGGCGGTCCAGGCCAAAAAAACCGAGGCCGCAAATTCCTGGTCCTCCTCCTTCTAAAGGACCAATTATTGAACACCAGAAAAAGATTGGTCTTTGGGCTGTGAAGCTACCTTCAGCAGATGCTATTGTTGTACGAAGAACACTTGGTGCTCTGACTGAAAATCCTGGTGGTCTTCCAGGGAGGAATGAGACTGATGAACATATTAAGAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTTGGGGTGAAGATGGGCACCAAGTCTCTGTTGGGCATTATTCGGATCATGATTGTTGGACTCTTTCTTGGGCTCTTGGGTCAATTTTCCTTTGGGAGGTGGCTGCTTTTAAAGTTTCCCTCATTCTTCAGTGTTGGATGGTTCAGAAAGGAGGGTCCCTCAGATGAGGAGGTGAGAAGTGCTTCTTTTAAGATGTGGTTTGTTGGACATGGTTTCAGCGACAGGAGTCTTGTTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAATTGGATACCTAACGACCCCGATAATTCTACTTCAATGTGCTCTTATTGTTTTGAGCCAACGTGACGACCTTCCAAAGGGAGGAGTTTTCCCAGCTGGGATTGTATTTGGACCAACTGATCTTCAAGAAAGGCTTCAAGAAAATGGAGTTACTTTTGATGTTATATCGAAGAATGCTATTTCTCATTAA

Protein Analysis

454

Amino Acids

49.34

Weight (kDa)

8.57

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 13 - 146 1.5e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 589
AatII GACGTC 1 cut(s) 8
Acc65I GGTACC 1 cut(s) 517
AccB1I GGYRCC 3 cut(s) 47, 517, 899
AclWI GGATC 1 cut(s) 935
AcoI YGGCCR 2 cut(s) 139, 609
AcsI RAATTY 1 cut(s) 669
AcuI CTGAAG 2 cut(s) 732, 994
AcyI GRCGYC 2 cut(s) 5, 48
AdeI CACNNNGTG 1 cut(s) 782
AfaI GTAC 3 cut(s) 71, 519, 768
AfiI CCNNNNNNNGG 7 cut(s) 174, 175, 692, 978, 1175, 1249, 1253
AgsI TTSAA 4 cut(s) 707, 1208, 1298, 1310
AhdI GACNNNNNGTC 1 cut(s) 241
AjnI CCWGG 4 cut(s) 644, 673, 799, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 7 cut(s) 157, 475, 546, 634, 742, 878, 1268
AluI AGCT 7 cut(s) 157, 475, 546, 634, 742, 878, 1268
Alw21I GWGCWC 3 cut(s) 636, 787, 1216
Alw26I GTCTC 2 cut(s) 818, 912
AlwI GGATC 1 cut(s) 935
Ama87I CYCGRG 1 cut(s) 493
AoxI GGCC 5 cut(s) 139, 177, 609, 647, 662
ApeKI GCWGC 3 cut(s) 326, 345, 985
ApoI RAATTY 1 cut(s) 669
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
Asp700I GAANNNNTTC 2 cut(s) 1063, 1305
Asp718I GGTACC 1 cut(s) 517
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 8 cut(s) 177, 293, 642, 677, 695, 1037, 1166, 1283
AsuHPI GGTGA 6 cut(s) 52, 105, 112, 211, 901, 1066
AvaI CYCGRG 1 cut(s) 493
AvaII GGWCC 7 cut(s) 293, 642, 677, 695, 1037, 1166, 1283
BaeGI GKGCMC 1 cut(s) 902
BalI TGGCCA 1 cut(s) 611
BanI GGYRCC 3 cut(s) 47, 517, 899
BanII GRGCYC 3 cut(s) 318, 636, 957
BbsI GAAGAC 1 cut(s) 800
Bbv12I GWGCWC 3 cut(s) 636, 787, 1216
BbvI GCAGC 3 cut(s) 313, 332, 972
BccI CCATC 5 cut(s) 221, 385, 622, 889, 1014
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 4 cut(s) 646, 675, 801, 813
BciVI GTATCC 1 cut(s) 1172
BcoDI GTCTC 2 cut(s) 818, 912
BfoI RGCGCY 1 cut(s) 51
BfuI GTATCC 1 cut(s) 1172
BglI GCCNNNNNGGC 1 cut(s) 309
Bme1390I CCNGG 4 cut(s) 646, 675, 801, 813
Bme18I GGWCC 7 cut(s) 293, 642, 677, 695, 1037, 1166, 1283
BmeRI GACNNNNNGTC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 493
BmgT120I GGNCC 8 cut(s) 177, 293, 642, 677, 695, 1037, 1166, 1283
BmiI GGNNCC 8 cut(s) 49, 294, 295, 519, 901, 1038, 1039, 1167
BmrFI CCNGG 4 cut(s) 646, 675, 801, 813
BmsI GCATC 1 cut(s) 745
BoxI GACNNNNGTC 1 cut(s) 1107
BpiI GAAGAC 1 cut(s) 800
BplI GAGNNNNNCTC 2 cut(s) 1025, 1057
Bpu10I CCTNAGC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 74
BsaHI GRCGYC 2 cut(s) 5, 48
BsaJI CCNNGG 3 cut(s) 43, 659, 812
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 7 cut(s) 174, 175, 692, 978, 1175, 1249, 1253
Bse118I RCCGGY 2 cut(s) 62, 137
BseBI CCWGG 4 cut(s) 646, 675, 801, 813
BseDI CCNNGG 3 cut(s) 43, 659, 812
BseGI GGATG 4 cut(s) 39, 213, 396, 1025
BseLI CCNNNNNNNGG 7 cut(s) 174, 175, 692, 978, 1175, 1249, 1253
BseMII CTCAG 3 cut(s) 254, 1056, 1133
BseRI GAGGAG 5 cut(s) 12, 100, 672, 1064, 1269
BseSI GKGCMC 1 cut(s) 902
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 313, 332, 972
BseYI CCCAGC 2 cut(s) 1264, 1268
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 5 cut(s) 141, 179, 611, 649, 664
BshNI GGYRCC 3 cut(s) 47, 517, 899
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 3 cut(s) 636, 787, 1216
BsiHKCI CYCGRG 1 cut(s) 493
BsiSI CCGG 6 cut(s) 54, 63, 138, 374, 467, 637
BslFI GGGAC 5 cut(s) 229, 306, 595, 1023, 1179
BslI CCNNNNNNNGG 7 cut(s) 174, 175, 692, 978, 1175, 1249, 1253
BsmAI GTCTC 2 cut(s) 818, 912
BsmFI GGGAC 5 cut(s) 229, 306, 595, 1023, 1179
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 5 cut(s) 141, 179, 611, 649, 664
BsoBI CYCGRG 1 cut(s) 493
Bsp1286I GDGCHC 6 cut(s) 318, 636, 787, 902, 957, 1216
Bsp143I GATC 2 cut(s) 927, 1291
BspANI GGCC 5 cut(s) 141, 179, 611, 649, 664
BspCNI CTCAG 3 cut(s) 253, 1055, 1132
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 8 cut(s) 49, 294, 295, 519, 901, 1038, 1039, 1167
BspPI GGATC 1 cut(s) 935
BspT107I GGYRCC 3 cut(s) 47, 517, 899
BsrFI RCCGGY 2 cut(s) 62, 137
BssAI RCCGGY 2 cut(s) 62, 137
BssECI CCNNGG 3 cut(s) 43, 659, 812
BssMI GATC 2 cut(s) 927, 1291
BssNI GRCGYC 2 cut(s) 5, 48
Bst2UI CCWGG 4 cut(s) 646, 675, 801, 813
Bst4CI ACNGT 1 cut(s) 868
BstACI GRCGYC 2 cut(s) 5, 48
BstC8I GCNNGC 5 cut(s) 139, 314, 331, 448, 876
BstDEI CTNAG 4 cut(s) 133, 240, 1042, 1119
BstENI CCTNNNNNAGG 3 cut(s) 690, 976, 1247
BstF5I GGATG 4 cut(s) 39, 213, 396, 1025
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 2 cut(s) 930, 1294
BstMAI GTCTC 2 cut(s) 818, 912
BstMBI GATC 2 cut(s) 927, 1291
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 6 cut(s) 54, 63, 258, 309, 339, 646
BstNI CCWGG 4 cut(s) 646, 675, 801, 813
BstNSI RCATGY 2 cut(s) 333, 450
BstPAI GACNNNNGTC 1 cut(s) 1107
BstSCI CCNGG 4 cut(s) 644, 673, 799, 811
BstSLI GKGCMC 1 cut(s) 902
BstV1I GCAGC 3 cut(s) 313, 332, 972
BstV2I GAAGAC 1 cut(s) 800
BstZI CGGCCG 1 cut(s) 139
BsuI GTATCC 1 cut(s) 1172
BsuRI GGCC 5 cut(s) 141, 179, 611, 649, 664
BtsCI GGATG 4 cut(s) 39, 213, 396, 1025
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 3 cut(s) 506, 873, 1018
Cac8I GCNNGC 5 cut(s) 139, 314, 331, 448, 876
CciI TCATGA 1 cut(s) 930
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 2 cut(s) 62, 137
Cfr13I GGNCC 8 cut(s) 177, 293, 642, 677, 695, 1037, 1166, 1283
Csp6I GTAC 3 cut(s) 70, 518, 767
CviAII CATG 6 cut(s) 174, 330, 382, 447, 931, 1091
CviQI GTAC 3 cut(s) 70, 518, 767
DdeI CTNAG 4 cut(s) 133, 240, 1042, 1119
DinI GGCGCC 1 cut(s) 49
DpnI GATC 2 cut(s) 929, 1293
DpnII GATC 2 cut(s) 927, 1291
DraI TTTAAA 1 cut(s) 993
DraIII CACNNNGTG 1 cut(s) 782
DrdI GACNNNNNNGTC 1 cut(s) 589
DriI GACNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 589
EaeI YGGCCR 2 cut(s) 139, 609
EagI CGGCCG 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 241
EciI GGCGGA 4 cut(s) 239, 290, 434, 485
Ecl136II GAGCTC 1 cut(s) 634
EclXI CGGCCG 1 cut(s) 139
Eco24I GRGCYC 3 cut(s) 318, 636, 957
Eco47I GGWCC 7 cut(s) 293, 642, 677, 695, 1037, 1166, 1283
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 2 cut(s) 732, 994
Eco88I CYCGRG 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 634
EcoNI CCTNNNNNAGG 3 cut(s) 690, 976, 1247
EcoO109I RGGNCCY 3 cut(s) 293, 1037, 1166
EcoRII CCWGG 4 cut(s) 644, 673, 799, 811
EcoT38I GRGCYC 3 cut(s) 318, 636, 957
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
FaeI CATG 6 cut(s) 177, 333, 385, 450, 934, 1094
FalI AAGNNNNNCTT 4 cut(s) 1051, 1083, 1290, 1322
FaqI GGGAC 5 cut(s) 229, 306, 595, 1023, 1179
FatI CATG 6 cut(s) 173, 329, 381, 446, 930, 1090
FauI CCCGC 2 cut(s) 443, 562
FauNDI CATATG 1 cut(s) 362
FokI GGATG 4 cut(s) 26, 200, 403, 1032
FriOI GRGCYC 3 cut(s) 318, 636, 957
GlaI GCGC 1 cut(s) 49
GsaI CCCAGC 2 cut(s) 1268, 1272
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 5 cut(s) 141, 179, 611, 649, 664
HapII CCGG 6 cut(s) 54, 63, 138, 374, 467, 637
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 2 cut(s) 5, 48
Hin1II CATG 6 cut(s) 177, 333, 385, 450, 934, 1094
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HinfI GANTC 4 cut(s) 458, 590, 942, 1108
HpaII CCGG 6 cut(s) 54, 63, 138, 374, 467, 637
HphI GGTGA 6 cut(s) 52, 105, 112, 211, 901, 1066
Hpy166II GTNNAC 1 cut(s) 864
Hpy188I TCNGA 6 cut(s) 10, 235, 789, 927, 1028, 1045
Hpy188III TCNNGA 4 cut(s) 356, 931, 1298, 1310
Hpy8I GTNNAC 1 cut(s) 864
Hpy99I CGWCG 3 cut(s) 77, 323, 326
HpyAV CCTTC 6 cut(s) 307, 418, 696, 756, 865, 1253
HpyCH4III ACNGT 1 cut(s) 868
HpyCH4IV ACGT 4 cut(s) 5, 72, 584, 1234
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 63, 258, 309, 339, 646
HpyF3I CTNAG 4 cut(s) 133, 240, 1042, 1119
HpySE526I ACGT 4 cut(s) 5, 72, 584, 1234
Hsp92I GRCGYC 2 cut(s) 5, 48
Hsp92II CATG 6 cut(s) 177, 333, 385, 450, 934, 1094
HspAI GCGC 1 cut(s) 48
KasI GGCGCC 1 cut(s) 47
KflI GGGWCCC 2 cut(s) 293, 1037
KpnI GGTACC 1 cut(s) 521
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 927, 1291
LmnI GCTCC 4 cut(s) 266, 472, 631, 639
Lsp1109I GCAGC 3 cut(s) 313, 332, 972
LweI GCATC 1 cut(s) 745
MaeII ACGT 4 cut(s) 5, 72, 584, 1234
MaeIII GTNAC 4 cut(s) 401, 1150, 1235, 1321
MalI GATC 2 cut(s) 929, 1293
MboI GATC 2 cut(s) 927, 1291
MboII GAAGA 6 cut(s) 783, 800, 904, 1000, 1286, 1354
MhlI GDGCHC 6 cut(s) 318, 636, 787, 902, 957, 1216
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 5 cut(s) 669, 699, 965, 1173, 1197
MluNI TGGCCA 1 cut(s) 611
Mly113I GGCGCC 1 cut(s) 48
MlyI GAGTC 4 cut(s) 452, 599, 936, 1117
MmeI TCCRAC 4 cut(s) 258, 919, 997, 1066
Mox20I TGGCCA 1 cut(s) 611
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 2 cut(s) 1063, 1305
MscI TGGCCA 1 cut(s) 611
MseI TTAA 5 cut(s) 489, 840, 992, 1071, 1360
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 1268
MspI CCGG 6 cut(s) 54, 63, 138, 374, 467, 637
MspR9I CCNGG 4 cut(s) 646, 675, 801, 813
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 4 cut(s) 646, 675, 801, 813
MwoI GCNNNNNNNGC 6 cut(s) 54, 63, 258, 309, 339, 646
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 1 cut(s) 48
NdeI CATATG 1 cut(s) 362
NdeII GATC 2 cut(s) 927, 1291
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 6 cut(s) 177, 333, 385, 450, 934, 1094
NlaIV GGNNCC 8 cut(s) 49, 294, 295, 519, 901, 1038, 1039, 1167
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 1235
NspI RCATGY 2 cut(s) 333, 450
PaeI GCATGC 2 cut(s) 333, 450
PaeR7I CTCGAG 1 cut(s) 493
PagI TCATGA 1 cut(s) 930
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 330
PctI GAATGC 1 cut(s) 1351
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 2 cut(s) 1063, 1305
PleI GAGTC 4 cut(s) 452, 598, 936, 1116
PluTI GGCGCC 1 cut(s) 51
PpsI GAGTC 4 cut(s) 452, 598, 936, 1116
PpuMI RGGWCCY 3 cut(s) 293, 1037, 1166
PshAI GACNNNNGTC 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 636
Psp5II RGGWCCY 3 cut(s) 293, 1037, 1166
Psp6I CCWGG 4 cut(s) 644, 673, 799, 811
PspFI CCCAGC 2 cut(s) 1264, 1268
PspGI CCWGG 4 cut(s) 644, 673, 799, 811
PspN4I GGNNCC 8 cut(s) 49, 294, 295, 519, 901, 1038, 1039, 1167
PspPI GGNCC 8 cut(s) 177, 293, 642, 677, 695, 1037, 1166, 1283
PspPPI RGGWCCY 3 cut(s) 293, 1037, 1166
PspXI VCTCGAGB 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 1268
RsaI GTAC 3 cut(s) 71, 519, 768
RsaNI GTAC 3 cut(s) 70, 518, 767
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 5 cut(s) 489, 840, 992, 1071, 1360
Sau3AI GATC 2 cut(s) 927, 1291
Sau96I GGNCC 8 cut(s) 177, 293, 642, 677, 695, 1037, 1166, 1283
SchI GAGTC 4 cut(s) 452, 599, 936, 1117
ScrFI CCNGG 4 cut(s) 646, 675, 801, 813
SduI GDGCHC 6 cut(s) 318, 636, 787, 902, 957, 1216
SfaNI GCATC 1 cut(s) 745
SfoI GGCGCC 1 cut(s) 49
Sfr274I CTCGAG 1 cut(s) 493
SinI GGWCC 7 cut(s) 293, 642, 677, 695, 1037, 1166, 1283
SlaI CTCGAG 1 cut(s) 493
SmlI CTYRAG 2 cut(s) 89, 493
SmoI CTYRAG 2 cut(s) 89, 493
SphI GCATGC 2 cut(s) 333, 450
Sse9I AATT 5 cut(s) 669, 699, 965, 1173, 1197
SspDI GGCGCC 1 cut(s) 47
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 4 cut(s) 644, 673, 799, 811
TaaI ACNGT 1 cut(s) 868
TaiI ACGT 4 cut(s) 8, 75, 587, 1237
TaqI TCGA 3 cut(s) 357, 494, 1340
TasI AATT 5 cut(s) 669, 699, 965, 1173, 1197
TauI GCSGC 8 cut(s) 144, 182, 185, 255, 345, 419, 515, 667
Tru1I TTAA 5 cut(s) 489, 840, 992, 1071, 1360
Tru9I TTAA 5 cut(s) 489, 840, 992, 1071, 1360
TscAI CASTG 3 cut(s) 506, 873, 1018
TseFI GTSAC 1 cut(s) 1235
TseI GCWGC 3 cut(s) 326, 345, 985
Tsp45I GTSAC 1 cut(s) 1235
TspDTI ATGAA 2 cut(s) 370, 846
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 3 cut(s) 506, 873, 1018
VpaK11BI GGWCC 7 cut(s) 293, 642, 677, 695, 1037, 1166, 1283
XagI CCTNNNNNAGG 3 cut(s) 690, 976, 1247
XapI RAATTY 1 cut(s) 669
XceI RCATGY 2 cut(s) 333, 450
XcmI CCANNNNNNNNNTGG 2 cut(s) 719, 910
XhoI CTCGAG 1 cut(s) 493
XmnI GAANNNNTTC 2 cut(s) 1063, 1305
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.