Rroxscaffold_2G00084590

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002135
Physical Location & Seq
Forward (+)
7131670 .. 7133733
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084590.1

Sequence Viewer

Length: 1290 bp
ATGTCAGACCTCCCCAACCCTCTCTATGATTTCATCATCCTCGGCGCCTCCGGCTTCACTGGAAACCGGCCGCAACCCCGCAAAAAGCTCTCCCAAACTCTCAACTGGGCCACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGACCCTCCGTCCCTCCGCCGCCTCTGCTCCCAAACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAGCCCGTCGTCGCTGCGTGCGTTGAGACCGGCTGTGATTATCTCGACATATCCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAGGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTCGACTCTGTTCCAGCAGAGTTTGGGTTGATGTTTAACTCGAGGCAGTGGGTGGGGGCGGCGGTACCGAATAGGGTGGAGGCTTATGTGAGCTTGGAGTCTGAGAAGAGACTTGTTTTGAACTTCGGGACGTTTGAGTCGGCGGTGTTGAGTGTGGCAAATGCGGATAAGTTGATGGAGCTCCGGCGGTCCAGACCTAAAAAGCCGAGGCCGGAGATTCCTGGTCCTCCTCCTTCTGAAGGACCAACCATTGAACACCAGAAAAAGATTGGCCTTTGGGCTGTGAAGCTACCTTCAGTAGATACTATCGTTGTACGAAGAACACTTGGAGCTCTGACTGAAAATCCCGGTGGTCTTCCAGGGAGGAATGAGAGTGATGAACACATTAAAAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTCGGGGTGAAGATAGGCACCAAGTCTCTGTTGGGCATTTTTCGGATGATGATAATTGGAATCTTTCTTGGGCTCTTGGGTCAGTTTTCCTTTGGGAGGCGGCTTCTCTTAAAGTATCCCTCATTTTTCAGTGTTGGATGGTTCAGGAAGCAGGGTCCCTCCGATGAGGAGGTGAGAAGTGCATCTTTTAAGATGTGGTTTGTTGGACGTGGTTTCAGCGACAGGAGTCTTACTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAGTTGGCTACATAACAACCCCGATAATTCTACTTCAGTGTGCACTTATTGTTCTGAGCCAACGCGACAATCTTCCAGAGGGAGGAGTTCTCCCACCTGGGATTGTATTTGGCCCAACTGATCTCCAAGAAAAGTTACAAGAAAATGGAGTTACTTTTGATGTTATTTCAAAGAATGTTATTTCTCATTAA

Protein Analysis

429

Amino Acids

47.24

Weight (kDa)

8.79

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 48 - 122 7.5e-07 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 517
Acc65I GGTACC 1 cut(s) 445
AccB1I GGYRCC 3 cut(s) 44, 445, 827
AccII CGCG 1 cut(s) 1164
AccIII TCCGGA 1 cut(s) 293
AcoI YGGCCR 1 cut(s) 68
AcuI CTGAAG 4 cut(s) 639, 660, 1118, 1119
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 447, 696
AfiI CCNNNNNNNGG 4 cut(s) 620, 906, 1103, 1181
AgsI TTSAA 3 cut(s) 502, 635, 1269
AhdI GACNNNNNGTC 1 cut(s) 169
AjiI CACGTC 1 cut(s) 1019
AjnI CCWGG 3 cut(s) 601, 739, 1195
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 6 cut(s) 88, 474, 562, 670, 713, 806
AluI AGCT 6 cut(s) 88, 474, 562, 670, 713, 806
Alw21I GWGCWC 3 cut(s) 564, 715, 1144
Alw26I GTCTC 3 cut(s) 260, 484, 840
Alw44I GTGCAC 1 cut(s) 1140
Ama87I CYCGRG 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 8 cut(s) 68, 108, 115, 220, 324, 590, 652, 1210
ApaI GGGCCC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 1140
ApeKI GCWGC 1 cut(s) 254
Asp718I GGTACC 1 cut(s) 445
AspLEI GCGC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 3 cut(s) 139, 829, 994
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 5 cut(s) 570, 605, 623, 965, 1094
BaeGI GKGCMC 2 cut(s) 224, 1144
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BanI GGYRCC 3 cut(s) 44, 445, 827
BanII GRGCYC 6 cut(s) 224, 246, 359, 564, 715, 885
BbsI GAAGAC 1 cut(s) 728
Bbv12I GWGCWC 3 cut(s) 564, 715, 1144
BbvI GCAGC 1 cut(s) 241
BccI CCATC 4 cut(s) 149, 313, 550, 942
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 3 cut(s) 603, 741, 1197
BciVI GTATCC 1 cut(s) 936
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 3 cut(s) 260, 484, 840
BfoI RGCGCY 1 cut(s) 48
BfuI GTATCC 1 cut(s) 936
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 6 cut(s) 71, 181, 255, 345, 441, 911
BlsI GCNGC 6 cut(s) 72, 182, 256, 346, 442, 912
Bme1390I CCNGG 4 cut(s) 603, 729, 741, 1197
Bme18I GGWCC 5 cut(s) 570, 605, 623, 965, 1094
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 1019
BmiI GGNNCC 8 cut(s) 46, 222, 223, 447, 829, 966, 967, 1095
BmrFI CCNGG 4 cut(s) 603, 729, 741, 1197
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 1 cut(s) 1001
BmuI ACTGGG 1 cut(s) 115
BoxI GACNNNNGTC 1 cut(s) 1035
BpiI GAAGAC 1 cut(s) 728
BplI GAGNNNNNCTC 4 cut(s) 341, 373, 1173, 1205
BpuMI CCSGG 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 4 cut(s) 40, 587, 740, 1196
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 4 cut(s) 620, 906, 1103, 1181
Bse118I RCCGGY 3 cut(s) 66, 113, 269
Bse1I ACTGG 3 cut(s) 64, 110, 327
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 3 cut(s) 603, 741, 1197
BseDI CCNNGG 4 cut(s) 40, 587, 740, 1196
BseGI GGATG 6 cut(s) 36, 141, 204, 324, 861, 953
BseLI CCNNNNNNNGG 4 cut(s) 620, 906, 1103, 1181
BseMII CTCAG 3 cut(s) 474, 1061, 1145
BseNI ACTGG 3 cut(s) 64, 110, 327
BseRI GAGGAG 3 cut(s) 600, 992, 1197
BseSI GKGCMC 2 cut(s) 224, 1144
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 1164
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 8 cut(s) 70, 110, 117, 222, 326, 592, 654, 1212
BshNI GGYRCC 3 cut(s) 44, 445, 827
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 3 cut(s) 564, 715, 1144
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 9 cut(s) 51, 67, 114, 270, 294, 302, 565, 593, 729
BslFI GGGAC 4 cut(s) 157, 523, 951, 1107
BslI CCNNNNNNNGG 4 cut(s) 620, 906, 1103, 1181
BsmAI GTCTC 3 cut(s) 260, 484, 840
BsmFI GGGAC 4 cut(s) 157, 523, 951, 1107
BsnI GGCC 8 cut(s) 70, 110, 117, 222, 326, 592, 654, 1212
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 421
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 7 cut(s) 224, 246, 359, 564, 715, 885, 1144
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 1 cut(s) 1219
BspANI GGCC 8 cut(s) 70, 110, 117, 222, 326, 592, 654, 1212
BspCNI CTCAG 3 cut(s) 475, 1060, 1146
BspEI TCCGGA 1 cut(s) 293
BspFNI CGCG 1 cut(s) 1164
BspLI GGNNCC 8 cut(s) 46, 222, 223, 447, 829, 966, 967, 1095
BspT107I GGYRCC 3 cut(s) 44, 445, 827
BspTNI GGTCTC 1 cut(s) 260
BsrFI RCCGGY 3 cut(s) 66, 113, 269
BsrI ACTGG 3 cut(s) 64, 110, 327
BssAI RCCGGY 3 cut(s) 66, 113, 269
BssECI CCNNGG 4 cut(s) 40, 587, 740, 1196
BssMI GATC 1 cut(s) 1219
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 603, 741, 1197
Bst4CI ACNGT 1 cut(s) 796
Bst6I CTCTTC 1 cut(s) 482
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 242, 259, 804
BstDEI CTNAG 3 cut(s) 483, 1047, 1154
BstENI CCTNNNNNAGG 2 cut(s) 618, 904
BstF5I GGATG 6 cut(s) 36, 141, 204, 324, 861, 953
BstFNI CGCG 1 cut(s) 1164
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 1 cut(s) 1222
BstMAI GTCTC 3 cut(s) 260, 484, 840
BstMBI GATC 1 cut(s) 1219
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 4 cut(s) 51, 186, 228, 237
BstNI CCWGG 3 cut(s) 603, 741, 1197
BstPAI GACNNNNGTC 1 cut(s) 1035
BstSCI CCNGG 4 cut(s) 601, 727, 739, 1195
BstSLI GKGCMC 2 cut(s) 224, 1144
BstUI CGCG 1 cut(s) 1164
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 728
BstZI CGGCCG 1 cut(s) 68
BsuI GTATCC 1 cut(s) 936
BsuRI GGCC 8 cut(s) 70, 110, 117, 222, 326, 592, 654, 1212
BtrI CACGTC 1 cut(s) 1019
BtsCI GGATG 6 cut(s) 36, 141, 204, 324, 861, 953
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 5 cut(s) 57, 434, 801, 946, 1142
Cac8I GCNNGC 3 cut(s) 242, 259, 804
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 3 cut(s) 66, 113, 269
Csp6I GTAC 2 cut(s) 446, 695
CviAII CATG 1 cut(s) 310
CviQI GTAC 2 cut(s) 446, 695
DdeI CTNAG 3 cut(s) 483, 1047, 1154
DinI GGCGCC 1 cut(s) 46
DpnI GATC 1 cut(s) 1221
DpnII GATC 1 cut(s) 1219
DrdI GACNNNNNNGTC 1 cut(s) 517
DriI GACNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 68
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 482
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 482
EciI GGCGGA 3 cut(s) 167, 218, 362
Ecl136II GAGCTC 2 cut(s) 562, 713
EclXI CGGCCG 1 cut(s) 68
Eco24I GRGCYC 6 cut(s) 224, 246, 359, 564, 715, 885
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 5 cut(s) 570, 605, 623, 965, 1094
Eco52I CGGCCG 1 cut(s) 68
Eco53kI GAGCTC 2 cut(s) 562, 713
Eco57I CTGAAG 4 cut(s) 639, 660, 1118, 1119
Eco88I CYCGRG 1 cut(s) 421
EcoICRI GAGCTC 2 cut(s) 562, 713
EcoNI CCTNNNNNAGG 2 cut(s) 618, 904
EcoO109I RGGNCCY 3 cut(s) 221, 965, 1094
EcoRII CCWGG 3 cut(s) 601, 739, 1195
EcoT38I GRGCYC 6 cut(s) 224, 246, 359, 564, 715, 885
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 1 cut(s) 313
FaiI YATR 7 cut(s) 27, 290, 311, 468, 1070, 1091, 1112
FalI AAGNNNNNCTT 2 cut(s) 979, 1011
FaqI GGGAC 4 cut(s) 157, 523, 951, 1107
FatI CATG 1 cut(s) 309
FauI CCCGC 2 cut(s) 86, 206
Fnu4HI GCNGC 6 cut(s) 71, 181, 255, 345, 441, 911
FokI GGATG 6 cut(s) 23, 128, 191, 331, 868, 960
FriOI GRGCYC 6 cut(s) 224, 246, 359, 564, 715, 885
Fsp4HI GCNGC 6 cut(s) 71, 181, 255, 345, 441, 911
GlaI GCGC 1 cut(s) 46
GluI GCNGC 6 cut(s) 71, 181, 255, 345, 441, 911
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 8 cut(s) 70, 110, 117, 222, 326, 592, 654, 1212
HapII CCGG 9 cut(s) 51, 67, 114, 270, 294, 302, 565, 593, 729
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HinfI GANTC 6 cut(s) 386, 479, 518, 598, 870, 1036
HpaII CCGG 9 cut(s) 51, 67, 114, 270, 294, 302, 565, 593, 729
HphI GGTGA 3 cut(s) 139, 829, 994
Hpy166II GTNNAC 2 cut(s) 792, 1142
Hpy188I TCNGA 7 cut(s) 7, 484, 619, 717, 855, 973, 1155
Hpy188III TCNNGA 6 cut(s) 284, 294, 508, 573, 955, 1175
Hpy8I GTNNAC 2 cut(s) 792, 1142
Hpy99I CGWCG 2 cut(s) 251, 254
HpyAV CCTTC 6 cut(s) 235, 346, 614, 624, 684, 793
HpyCH4III ACNGT 1 cut(s) 796
HpyCH4IV ACGT 2 cut(s) 512, 1018
HpyCH4V TGCA 2 cut(s) 992, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 186, 228, 237
HpyF3I CTNAG 3 cut(s) 483, 1047, 1154
HpySE526I ACGT 2 cut(s) 512, 1018
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KflI GGGWCCC 1 cut(s) 965
Kpn2I TCCGGA 1 cut(s) 293
KpnI GGTACC 1 cut(s) 449
Kzo9I GATC 1 cut(s) 1219
LmnI GCTCC 4 cut(s) 194, 559, 567, 710
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 1001
MaeII ACGT 2 cut(s) 512, 1018
MaeIII GTNAC 3 cut(s) 1078, 1233, 1249
MalI GATC 1 cut(s) 1221
MboI GATC 1 cut(s) 1219
MboII GAAGA 5 cut(s) 499, 711, 728, 832, 1163
MhlI GDGCHC 7 cut(s) 224, 246, 359, 564, 715, 885, 1144
MluCI AATT 2 cut(s) 864, 1125
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 4 cut(s) 380, 488, 527, 1045
MmeI TCCRAC 2 cut(s) 925, 994
MroI TCCGGA 1 cut(s) 293
MseI TTAA 5 cut(s) 417, 768, 920, 999, 1288
MspI CCGG 9 cut(s) 51, 67, 114, 270, 294, 302, 565, 593, 729
MspR9I CCNGG 4 cut(s) 603, 729, 741, 1197
MvaI CCWGG 3 cut(s) 603, 741, 1197
MvnI CGCG 1 cut(s) 1164
MwoI GCNNNNNNNGC 4 cut(s) 51, 186, 228, 237
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 729
NdeII GATC 1 cut(s) 1219
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 8 cut(s) 46, 222, 223, 447, 829, 966, 967, 1095
NmeAIII GCCGAG 2 cut(s) 21, 612
PaeR7I CTCGAG 1 cut(s) 421
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 258
PfeI GAWTC 2 cut(s) 598, 870
PkrI GCNGC 6 cut(s) 72, 182, 256, 346, 442, 912
PleI GAGTC 4 cut(s) 380, 487, 526, 1044
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 4 cut(s) 380, 487, 526, 1044
PpuMI RGGWCCY 2 cut(s) 965, 1094
PshAI GACNNNNGTC 1 cut(s) 1035
Psp124BI GAGCTC 2 cut(s) 564, 715
Psp5II RGGWCCY 2 cut(s) 965, 1094
Psp6I CCWGG 3 cut(s) 601, 739, 1195
PspGI CCWGG 3 cut(s) 601, 739, 1195
PspN4I GGNNCC 8 cut(s) 46, 222, 223, 447, 829, 966, 967, 1095
PspOMI GGGCCC 1 cut(s) 220
PspPPI RGGWCCY 2 cut(s) 965, 1094
PspXI VCTCGAGB 1 cut(s) 421
RsaI GTAC 2 cut(s) 447, 696
RsaNI GTAC 2 cut(s) 446, 695
SacI GAGCTC 2 cut(s) 564, 715
SaqAI TTAA 5 cut(s) 417, 768, 920, 999, 1288
SatI GCNGC 6 cut(s) 71, 181, 255, 345, 441, 911
Sau3AI GATC 1 cut(s) 1219
SchI GAGTC 4 cut(s) 380, 488, 527, 1045
ScrFI CCNGG 4 cut(s) 603, 729, 741, 1197
SduI GDGCHC 7 cut(s) 224, 246, 359, 564, 715, 885, 1144
SfaNI GCATC 1 cut(s) 1001
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 421
SinI GGWCC 5 cut(s) 570, 605, 623, 965, 1094
SlaI CTCGAG 1 cut(s) 421
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 2 cut(s) 864, 1125
SspDI GGCGCC 1 cut(s) 44
SstI GAGCTC 2 cut(s) 564, 715
StyD4I CCNGG 4 cut(s) 601, 727, 739, 1195
TaaI ACNGT 1 cut(s) 796
TaiI ACGT 2 cut(s) 515, 1021
TaqI TCGA 4 cut(s) 285, 336, 384, 422
TasI AATT 2 cut(s) 864, 1125
TauI GCSGC 5 cut(s) 73, 183, 347, 443, 913
TfiI GAWTC 2 cut(s) 598, 870
Tru1I TTAA 5 cut(s) 417, 768, 920, 999, 1288
Tru9I TTAA 5 cut(s) 417, 768, 920, 999, 1288
TscAI CASTG 5 cut(s) 64, 434, 801, 946, 1142
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 3 cut(s) 22, 298, 774
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 5 cut(s) 64, 434, 801, 946, 1142
VneI GTGCAC 1 cut(s) 1140
VpaK11BI GGWCC 5 cut(s) 570, 605, 623, 965, 1094
XagI CCTNNNNNAGG 2 cut(s) 618, 904
XcmI CCANNNNNNNNNTGG 2 cut(s) 647, 838
XhoI CTCGAG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.