Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002135

Physical Location & Seq

Genomic Coordinates Forward (+)

4133841 .. 4136018

Gene Structure 2178 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1060600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGCCGGAATTTCAACCCCAAACCACCGCCAGCCCTCCTTCCTACGATCTCATAATCCTCGGCGCCTCCGGCTTCACCGGTAAGTACGTCGTCAGAGAAGCTCTGAAATTTCTGAACACGCCCGCCTCCCCTCTCAAGTCTCTAGCTTTAGCCGGTCGCAACCCAACCAAACTAACCCAAACCCTCAAATGGGCAGCCCACCCGAACACACCACCGTCCATCCCCATCATCACCGCCGACACCACCGATCCACCGTCTCTCCACCGCCTCTGCACCCAAACCACCCTCATCCTCAATTGCGTCGGCCCCTTCCGCCTCTACGGTGATCCCGTCGTCGCCGCCTGCGCCGAAACCGGCTGCGACTACTTGGATATATGCGGGGAGCCTGAGTTTATGGAGAGGATGGAGGCGCAGTACCATGGCAAGGCGGCGGAGACCGGTTCGTTGGTGGTCTCGGCATGTGGGTTCGACTCTGTTCCGGCGGAGTTTGGGTTGATGTTCAATTCGAGGCAGTGGGTCGACCCGGCTGTGCCGAACCGGGTTGAGGCTTATGTGAGCTTGGAGTCGGAGAAGAGGATTGTTGGGAATTTAGGGACGTATGAATCGGCGGTGCTGGGTGTGGCAAATGCCGATAAGTTGCAGGAGCTCCGGCGGTCCAGACCCAGAAGAGCTAGGCCTGCGATTCCTGGTCCTCCTCCTCCTAAAGGACCAACCATAGAACAGCAGAAAGATATTGGCCTTTGGGCTGTGAAGCTACCTTCAGCAGATGCTATCGTTGTACGTAGAACACTTGGATTTCTGACTGAAAACCCCCGCGGGCTTCCTGGGAGGAACGAGAGTTCTGAGCACATTGAAAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTTGGGGTGAAGATAGGAACCAAGTCACTGTTGGGCATTTTGCGTATCATGACAGTCGGAGTCTTTATTGGGCTTTTGGGTAGATTCTCCTTTGGGAGGTGGCTTCTTCTGAAATTTCCCTCTTTTTTCAGTGTTGGATGGTTCAGGAAGAAGGGTCCCTCCGAGGATGAGGTGAGAAGCGCTTCGTTTAAGATGTGGTTTGTTGGACATGGTTTCAGCGACAGCAGTCTTGTTTCGCAGGGAAGCAGAAAACCCAACATGGAAATAATAACAAGAGTGATGGGACCTGAGATTGGTTATTTGACGACCCCGATAATTCTACTTCAGTGTGCTCTGATTGTTCTGAGCCAACGCGACAACCTTCCAAAGGGAGGAGTTCTCCCAGCTGGCATTGTGTTTGGCCCAACTGGTCTTCAGGAAAGGCTCCAAGAGAACGGGATTTCTTTCGATGTCATTTCGAAGAAAGCTCTTTCTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0031090 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sacchrp_dh_NADP

Protein Analysis

458

Amino Acids

49.81

Weight (kDa)

9.23

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sacchrp_dh_NADP	PF03435	18 - 151	5.1e-12	Saccharopine dehydrogenase NADP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39410
fragaria_vesca	FvH4_6g47610 FvH4_6g47650 FvH4_6g47650 FvH4_6g47700
malus_domestica	MD09G1060600.v1.1
prunus_persica	Prupe.3G260300_v2.0.a1
pyrus_communis	pycom111g05100
rosa_chinensis	RchiOBHm_Chr2g0166731 RchiOBHm_Chr2g0166761 RchiOBHm_Chr2g0166771 RchiOBHm_Chr4g0415091
rosa_laevigata	RLG00000021643 RLG00000021646 RLG00000021664 RLG00000021667
rosa_multiflora	Rmu_co8040738.1_g000001 Rmu_sc0000949.1_g000002 Rmu_sc0000949.1_g000005 Rmu_sc0002181.1_g000013 Rmu_sc0002181.1_g000015 Rmu_sc0002181.1_g000024 Rmu_sc0004406.1_g000008
rosa_roxburghii	Rroxscaffold_2G00084230 Rroxscaffold_2G00084260 Rroxscaffold_2G00084280 Rroxscaffold_2G00084550 Rroxscaffold_2G00084590 Rroxscaffold_2G00084600
rosa_rugosa	Rorug02G0525000 Rorug02G0525200
rosa_samantha	Rh2AG591500 Rh2BG603200 Rh2BG603500 Rh2CG356500 Rh2CG574100 Rh2CG574500 Rh2DG614200 Rh2DG614300 Rh4BG125500
rosa_wichuraiana	Rw2G049260 Rw2G049280 Rw2G049300 Rw4G016320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	62
AccI	GTMKAC	1 cut(s)	519
AccII	CGCG	2 cut(s)	816, 1248
AclWI	GGATC	2 cut(s)	242, 320
AcsI	RAATTY	4 cut(s)	8, 107, 586, 1007
AcuI	CTGAAG	3 cut(s)	744, 1202, 1292
AcyI	GRCGYC	1 cut(s)	63
AfaI	GTAC	3 cut(s)	86, 416, 780
AfeI	AGCGCT	1 cut(s)	1075
AfiI	CCNNNNNNNGG	9 cut(s)	189, 190, 424, 544, 704, 990, 1187, 1261, 1265
AgeI	ACCGGT	2 cut(s)	77, 437
AgsI	TTSAA	3 cut(s)	14, 502, 854
AjnI	CCWGG	2 cut(s)	685, 823
AloI	GAACNNNNNNTCC	2 cut(s)	823, 855
AluBI	AGCT	9 cut(s)	101, 146, 558, 646, 671, 754, 890, 1280, 1361
AluI	AGCT	9 cut(s)	101, 146, 558, 646, 671, 754, 890, 1280, 1361
Alw21I	GWGCWC	3 cut(s)	648, 849, 1228
Alw26I	GTCTC	4 cut(s)	144, 261, 428, 457
AlwI	GGATC	2 cut(s)	242, 320
Aor51HI	AGCGCT	1 cut(s)	1075
AoxI	GGCC	4 cut(s)	304, 674, 736, 1294
ApeKI	GCWGC	2 cut(s)	194, 357
ApoI	RAATTY	4 cut(s)	8, 107, 586, 1007
AsiGI	ACCGGT	2 cut(s)	77, 437
Asp700I	GAANNNNTTC	1 cut(s)	1075
AspLEI	GCGC	4 cut(s)	65, 347, 412, 1076
AspS9I	GGNCC	7 cut(s)	305, 654, 689, 707, 1049, 1178, 1295
AsuC2I	CCSGG	2 cut(s)	524, 539
AsuHPI	GGTGA	5 cut(s)	67, 223, 335, 913, 1078
AsuII	TTCGAA	1 cut(s)	1352
AvaII	GGWCC	5 cut(s)	654, 689, 707, 1049, 1178
BanI	GGYRCC	1 cut(s)	62
BanII	GRGCYC	1 cut(s)	648
BbsI	GAAGAC	1 cut(s)	1298
Bbv12I	GWGCWC	3 cut(s)	648, 849, 1228
BbvI	GCAGC	2 cut(s)	206, 344
BccI	CCATC	5 cut(s)	227, 233, 397, 1026, 1168
BciT130I	CCWGG	2 cut(s)	687, 825
BcnI	CCSGG	2 cut(s)	524, 539
BcoDI	GTCTC	4 cut(s)	144, 261, 428, 457
BfaI	CTAG	2 cut(s)	143, 672
BfoI	RGCGCY	2 cut(s)	66, 1077
BisI	GCNGC	4 cut(s)	195, 339, 358, 429
BlsI	GCNGC	4 cut(s)	196, 340, 359, 430
Bme1390I	CCNGG	4 cut(s)	524, 539, 687, 825
Bme18I	GGWCC	5 cut(s)	654, 689, 707, 1049, 1178
BmgT120I	GGNCC	7 cut(s)	305, 654, 689, 707, 1049, 1178, 1295
BmiI	GGNNCC	8 cut(s)	64, 307, 384, 913, 1050, 1051, 1179, 1319
BmrFI	CCNGG	4 cut(s)	524, 539, 687, 825
BmsI	GCATC	1 cut(s)	757
BoxI	GACNNNNGTC	1 cut(s)	1119
BpiI	GAAGAC	1 cut(s)	1298
BplI	GAGNNNNNCTC	2 cut(s)	1257, 1289
Bpu14I	TTCGAA	1 cut(s)	1352
BpuEI	CTTGAG	1 cut(s)	119
BpuMI	CCSGG	2 cut(s)	524, 539
BsaAI	YACGTR	1 cut(s)	782
BsaHI	GRCGYC	1 cut(s)	63
BsaI	GGTCTC	2 cut(s)	428, 457
BsaJI	CCNNGG	5 cut(s)	58, 418, 814, 824, 1056
BsaWI	WCCGGW	2 cut(s)	77, 437
BsaXI	ACNNNNNCTCC	8 cut(s)	243, 273, 374, 398, 404, 428, 476, 506
Bsc4I	CCNNNNNNNGG	9 cut(s)	189, 190, 424, 544, 704, 990, 1187, 1261, 1265
Bse118I	RCCGGY	4 cut(s)	77, 152, 353, 437
Bse1I	ACTGG	1 cut(s)	1306
BseBI	CCWGG	2 cut(s)	687, 825
BseDI	CCNNGG	5 cut(s)	58, 418, 814, 824, 1056
BseGI	GGATG	5 cut(s)	219, 288, 408, 1037, 1066
BseLI	CCNNNNNNNGG	9 cut(s)	189, 190, 424, 544, 704, 990, 1187, 1261, 1265
BseMII	CTCAG	4 cut(s)	378, 834, 1173, 1229
BseNI	ACTGG	1 cut(s)	1306
BseRI	GAGGAG	3 cut(s)	684, 687, 1281
BseXI	GCAGC	2 cut(s)	206, 344
BseYI	CCCAGC	2 cut(s)	613, 1276
BsgI	GTGCAG	1 cut(s)	256
Bsh1236I	CGCG	2 cut(s)	816, 1248
Bsh1285I	CGRYCG	1 cut(s)	157
BshFI	GGCC	4 cut(s)	306, 676, 738, 1296
BshNI	GGYRCC	1 cut(s)	62
BshTI	ACCGGT	2 cut(s)	77, 437
BsiEI	CGRYCG	1 cut(s)	157
BsiHKAI	GWGCWC	3 cut(s)	648, 849, 1228
BslFI	GGGAC	3 cut(s)	607, 1035, 1191
BslI	CCNNNNNNNGG	9 cut(s)	189, 190, 424, 544, 704, 990, 1187, 1261, 1265
BsmAI	GTCTC	4 cut(s)	144, 261, 428, 457
BsmBI	CGTCTC	1 cut(s)	261
BsmFI	GGGAC	3 cut(s)	607, 1035, 1191
BsnI	GGCC	4 cut(s)	306, 676, 738, 1296
Bso31I	GGTCTC	2 cut(s)	428, 457
Bsp119I	TTCGAA	1 cut(s)	1352
Bsp1286I	GDGCHC	3 cut(s)	648, 849, 1228
Bsp143I	GATC	3 cut(s)	46, 247, 325
Bsp19I	CCATGG	1 cut(s)	418
BspANI	GGCC	4 cut(s)	306, 676, 738, 1296
BspCNI	CTCAG	4 cut(s)	379, 835, 1174, 1230
BspFNI	CGCG	2 cut(s)	816, 1248
BspHI	TCATGA	1 cut(s)	942
BspLI	GGNNCC	8 cut(s)	64, 307, 384, 913, 1050, 1051, 1179, 1319
BspPI	GGATC	2 cut(s)	242, 320
BspQI	GCTCTTC	1 cut(s)	661
BspT104I	TTCGAA	1 cut(s)	1352
BspT107I	GGYRCC	1 cut(s)	62
BspTNI	GGTCTC	2 cut(s)	428, 457
BsrFI	RCCGGY	4 cut(s)	77, 152, 353, 437
BsrI	ACTGG	1 cut(s)	1306
BssAI	RCCGGY	4 cut(s)	77, 152, 353, 437
BssECI	CCNNGG	5 cut(s)	58, 418, 814, 824, 1056
BssMI	GATC	3 cut(s)	46, 247, 325
BssNI	GRCGYC	1 cut(s)	63
BssT1I	CCWWGG	1 cut(s)	418
Bst2UI	CCWGG	2 cut(s)	687, 825
Bst4CI	ACNGT	6 cut(s)	216, 255, 323, 880, 924, 949
Bst6I	CTCTTC	2 cut(s)	566, 661
BstACI	GRCGYC	1 cut(s)	63
BstBAI	YACGTR	1 cut(s)	782
BstBI	TTCGAA	1 cut(s)	1352
BstC8I	GCNNGC	7 cut(s)	31, 123, 343, 678, 818, 888, 1282
BstDEI	CTNAG	4 cut(s)	387, 843, 1182, 1238
BstDSI	CCRYGG	2 cut(s)	418, 814
BstENI	CCTNNNNNAGG	3 cut(s)	702, 988, 1259
BstF5I	GGATG	5 cut(s)	219, 288, 408, 1037, 1066
BstFNI	CGCG	2 cut(s)	816, 1248
BstH2I	RGCGCY	2 cut(s)	66, 1077
BstHHI	GCGC	4 cut(s)	65, 347, 412, 1076
BstKTI	GATC	3 cut(s)	49, 250, 328
BstMAI	GTCTC	4 cut(s)	144, 261, 428, 457
BstMBI	GATC	3 cut(s)	46, 247, 325
BstMCI	CGRYCG	1 cut(s)	157
BstMWI	GCNNNNNNNGC	4 cut(s)	69, 312, 344, 677
BstNI	CCWGG	2 cut(s)	687, 825
BstNSI	RCATGY	1 cut(s)	462
BstPAI	GACNNNNGTC	1 cut(s)	1119
BstSCI	CCNGG	4 cut(s)	522, 537, 685, 823
BstSNI	TACGTA	1 cut(s)	782
BstUI	CGCG	2 cut(s)	816, 1248
BstV1I	GCAGC	2 cut(s)	206, 344
BstV2I	GAAGAC	1 cut(s)	1298
BsuRI	GGCC	4 cut(s)	306, 676, 738, 1296
BtgI	CCRYGG	2 cut(s)	418, 814
BtsCI	GGATG	5 cut(s)	219, 288, 408, 1037, 1066
BtsI	GCAGTG	1 cut(s)	518
BtsIMutI	CAGTG	5 cut(s)	518, 885, 920, 1030, 1226
Cac8I	GCNNGC	7 cut(s)	31, 123, 343, 678, 818, 888, 1282
CciI	TCATGA	1 cut(s)	942
CfoI	GCGC	4 cut(s)	65, 347, 412, 1076
Cfr10I	RCCGGY	4 cut(s)	77, 152, 353, 437
Cfr13I	GGNCC	7 cut(s)	305, 654, 689, 707, 1049, 1178, 1295
Cfr42I	CCGCGG	1 cut(s)	817
CseI	GACGC	1 cut(s)	289
Csp6I	GTAC	3 cut(s)	85, 415, 779
CspAI	ACCGGT	2 cut(s)	77, 437
CviAII	CATG	5 cut(s)	419, 459, 943, 1103, 1153
CviQI	GTAC	3 cut(s)	85, 415, 779
DdeI	CTNAG	4 cut(s)	387, 843, 1182, 1238
DinI	GGCGCC	1 cut(s)	64
DpnI	GATC	3 cut(s)	48, 249, 327
DpnII	GATC	3 cut(s)	46, 247, 325
Eam1104I	CTCTTC	2 cut(s)	566, 661
EarI	CTCTTC	2 cut(s)	566, 661
EciI	GGCGGA	3 cut(s)	302, 446, 497
Ecl136II	GAGCTC	1 cut(s)	646
Eco105I	TACGTA	1 cut(s)	782
Eco130I	CCWWGG	1 cut(s)	418
Eco147I	AGGCCT	1 cut(s)	676
Eco24I	GRGCYC	1 cut(s)	648
Eco31I	GGTCTC	2 cut(s)	428, 457
Eco47I	GGWCC	5 cut(s)	654, 689, 707, 1049, 1178
Eco47III	AGCGCT	1 cut(s)	1075
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	3 cut(s)	744, 1202, 1292
EcoICRI	GAGCTC	1 cut(s)	646
EcoNI	CCTNNNNNAGG	3 cut(s)	702, 988, 1259
EcoO109I	RGGNCCY	2 cut(s)	1049, 1178
EcoRII	CCWGG	2 cut(s)	685, 823
EcoT14I	CCWWGG	1 cut(s)	418
EcoT38I	GRGCYC	1 cut(s)	648
EgeI	GGCGCC	1 cut(s)	64
EheI	GGCGCC	1 cut(s)	64
ErhI	CCWWGG	1 cut(s)	418
Esp3I	CGTCTC	1 cut(s)	261
FaeI	CATG	5 cut(s)	422, 462, 946, 1106, 1156
FaqI	GGGAC	3 cut(s)	607, 1035, 1191
FatI	CATG	5 cut(s)	418, 458, 942, 1102, 1152
FauI	CCCGC	4 cut(s)	130, 371, 809, 821
FblI	GTMKAC	1 cut(s)	519
Fnu4HI	GCNGC	4 cut(s)	195, 339, 358, 429
FokI	GGATG	5 cut(s)	206, 275, 415, 1044, 1073
FriOI	GRGCYC	1 cut(s)	648
Fsp4HI	GCNGC	4 cut(s)	195, 339, 358, 429
FspBI	CTAG	2 cut(s)	143, 672
GlaI	GCGC	4 cut(s)	64, 346, 411, 1075
GluI	GCNGC	4 cut(s)	195, 339, 358, 429
GsaI	CCCAGC	2 cut(s)	617, 1280
HaeII	RGCGCY	2 cut(s)	66, 1077
HaeIII	GGCC	4 cut(s)	306, 676, 738, 1296
HgaI	GACGC	1 cut(s)	289
HhaI	GCGC	4 cut(s)	65, 347, 412, 1076
Hin1I	GRCGYC	1 cut(s)	63
Hin1II	CATG	5 cut(s)	422, 462, 946, 1106, 1156
Hin6I	GCGC	4 cut(s)	63, 345, 410, 1074
HinP1I	GCGC	4 cut(s)	63, 345, 410, 1074
HincII	GTYRAC	2 cut(s)	520, 876
HindII	GTYRAC	2 cut(s)	520, 876
HinfI	GANTC	6 cut(s)	470, 563, 602, 682, 954, 978
HphI	GGTGA	5 cut(s)	67, 223, 335, 913, 1078
Hpy166II	GTNNAC	2 cut(s)	520, 876
Hpy188III	TCNNGA	4 cut(s)	657, 943, 1039, 1310
Hpy8I	GTNNAC	2 cut(s)	520, 876
Hpy99I	CGWCG	4 cut(s)	92, 305, 335, 338
HpyAV	CCTTC	6 cut(s)	48, 319, 768, 877, 1039, 1265
HpyCH4III	ACNGT	6 cut(s)	216, 255, 323, 880, 924, 949
HpyCH4IV	ACGT	3 cut(s)	87, 596, 781
HpyCH4V	TGCA	2 cut(s)	273, 640
HpyF10VI	GCNNNNNNNGC	4 cut(s)	69, 312, 344, 677
HpyF3I	CTNAG	4 cut(s)	387, 843, 1182, 1238
HpySE526I	ACGT	3 cut(s)	87, 596, 781
Hsp92I	GRCGYC	1 cut(s)	63
Hsp92II	CATG	5 cut(s)	422, 462, 946, 1106, 1156
HspAI	GCGC	4 cut(s)	63, 345, 410, 1074
KasI	GGCGCC	1 cut(s)	62
KflI	GGGWCCC	1 cut(s)	1049
KspI	CCGCGG	1 cut(s)	817
Kzo9I	GATC	3 cut(s)	46, 247, 325
LguI	GCTCTTC	1 cut(s)	661
LmnI	GCTCC	4 cut(s)	382, 643, 651, 1323
Lsp1109I	GCAGC	2 cut(s)	206, 344
LweI	GCATC	1 cut(s)	757
MaeI	CTAG	2 cut(s)	143, 672
MaeII	ACGT	3 cut(s)	87, 596, 781
MaeIII	GTNAC	1 cut(s)	918
MalI	GATC	3 cut(s)	48, 249, 327
MboI	GATC	3 cut(s)	46, 247, 325
MboII	GAAGA	7 cut(s)	583, 678, 916, 992, 1054, 1298, 1366
MfeI	CAATTG	1 cut(s)	295
MhlI	GDGCHC	3 cut(s)	648, 849, 1228
MluCI	AATT	7 cut(s)	8, 107, 295, 502, 586, 1007, 1209
Mly113I	GGCGCC	1 cut(s)	63
MlyI	GAGTC	3 cut(s)	464, 572, 963
MmeI	TCCRAC	4 cut(s)	546, 931, 1009, 1078
MroXI	GAANNNNTTC	1 cut(s)	1075
MseI	TTAA	2 cut(s)	1083, 1372
MspA1I	CMGCKG	2 cut(s)	816, 1280
MspR9I	CCNGG	4 cut(s)	524, 539, 687, 825
MunI	CAATTG	1 cut(s)	295
MvaI	CCWGG	2 cut(s)	687, 825
MvnI	CGCG	2 cut(s)	816, 1248
MwoI	GCNNNNNNNGC	4 cut(s)	69, 312, 344, 677
NarI	GGCGCC	1 cut(s)	63
NciI	CCSGG	2 cut(s)	524, 539
NcoI	CCATGG	1 cut(s)	418
NdeII	GATC	3 cut(s)	46, 247, 325
NlaIII	CATG	5 cut(s)	422, 462, 946, 1106, 1156
NlaIV	GGNNCC	8 cut(s)	64, 307, 384, 913, 1050, 1051, 1179, 1319
NmeAIII	GCCGAG	2 cut(s)	39, 434
NmuCI	GTSAC	1 cut(s)	918
NspI	RCATGY	1 cut(s)	462
NspV	TTCGAA	1 cut(s)	1352
PagI	TCATGA	1 cut(s)	942
PceI	AGGCCT	1 cut(s)	676
PciSI	GCTCTTC	1 cut(s)	661
PcsI	WCGNNNNNNNCGW	1 cut(s)	327
PdmI	GAANNNNTTC	1 cut(s)	1075
PfeI	GAWTC	3 cut(s)	602, 682, 978
PinAI	ACCGGT	2 cut(s)	77, 437
PkrI	GCNGC	4 cut(s)	196, 340, 359, 430
PleI	GAGTC	3 cut(s)	464, 571, 962
PluTI	GGCGCC	1 cut(s)	66
PpsI	GAGTC	3 cut(s)	464, 571, 962
Ppu21I	YACGTR	1 cut(s)	782
PpuMI	RGGWCCY	2 cut(s)	1049, 1178
PshAI	GACNNNNGTC	1 cut(s)	1119
Psp124BI	GAGCTC	1 cut(s)	648
Psp5II	RGGWCCY	2 cut(s)	1049, 1178
Psp6I	CCWGG	2 cut(s)	685, 823
PspFI	CCCAGC	2 cut(s)	613, 1276
PspGI	CCWGG	2 cut(s)	685, 823
PspN4I	GGNNCC	8 cut(s)	64, 307, 384, 913, 1050, 1051, 1179, 1319
PspPI	GGNCC	7 cut(s)	305, 654, 689, 707, 1049, 1178, 1295
PspPPI	RGGWCCY	2 cut(s)	1049, 1178
PvuII	CAGCTG	1 cut(s)	1280
RsaI	GTAC	3 cut(s)	86, 416, 780
RsaNI	GTAC	3 cut(s)	85, 415, 779
SacI	GAGCTC	1 cut(s)	648
SacII	CCGCGG	1 cut(s)	817
SalI	GTCGAC	1 cut(s)	518
SapI	GCTCTTC	1 cut(s)	661
SaqAI	TTAA	2 cut(s)	1083, 1372
SatI	GCNGC	4 cut(s)	195, 339, 358, 429
Sau3AI	GATC	3 cut(s)	46, 247, 325
Sau96I	GGNCC	7 cut(s)	305, 654, 689, 707, 1049, 1178, 1295
SchI	GAGTC	3 cut(s)	464, 572, 963
ScrFI	CCNGG	4 cut(s)	524, 539, 687, 825
SduI	GDGCHC	3 cut(s)	648, 849, 1228
SfaNI	GCATC	1 cut(s)	757
SfoI	GGCGCC	1 cut(s)	64
Sfr303I	CCGCGG	1 cut(s)	817
SfuI	TTCGAA	1 cut(s)	1352
SgrBI	CCGCGG	1 cut(s)	817
SinI	GGWCC	5 cut(s)	654, 689, 707, 1049, 1178
SmlI	CTYRAG	1 cut(s)	134
SmoI	CTYRAG	1 cut(s)	134
SnaBI	TACGTA	1 cut(s)	782
Sse9I	AATT	7 cut(s)	8, 107, 295, 502, 586, 1007, 1209
SseBI	AGGCCT	1 cut(s)	676
SspDI	GGCGCC	1 cut(s)	62
SspMI	CTAG	2 cut(s)	143, 672
SstI	GAGCTC	1 cut(s)	648
StuI	AGGCCT	1 cut(s)	676
StyD4I	CCNGG	4 cut(s)	522, 537, 685, 823
StyI	CCWWGG	1 cut(s)	418
TaaI	ACNGT	6 cut(s)	216, 255, 323, 880, 924, 949
TaiI	ACGT	3 cut(s)	90, 599, 784
TaqI	TCGA	5 cut(s)	468, 506, 519, 1341, 1352
TasI	AATT	7 cut(s)	8, 107, 295, 502, 586, 1007, 1209
TauI	GCSGC	2 cut(s)	341, 431
TfiI	GAWTC	3 cut(s)	602, 682, 978
Tru1I	TTAA	2 cut(s)	1083, 1372
Tru9I	TTAA	2 cut(s)	1083, 1372
TscAI	CASTG	5 cut(s)	518, 885, 927, 1030, 1226
TseFI	GTSAC	1 cut(s)	918
TseI	GCWGC	2 cut(s)	194, 357
Tsp45I	GTSAC	1 cut(s)	918
TspDTI	ATGAA	1 cut(s)	615
TspRI	CASTG	5 cut(s)	518, 885, 927, 1030, 1226
VpaK11BI	GGWCC	5 cut(s)	654, 689, 707, 1049, 1178
XagI	CCTNNNNNAGG	3 cut(s)	702, 988, 1259
XapI	RAATTY	4 cut(s)	8, 107, 586, 1007
XceI	RCATGY	1 cut(s)	462
XcmI	CCANNNNNNNNNTGG	1 cut(s)	922
XmiI	GTMKAC	1 cut(s)	519
XmnI	GAANNNNTTC	1 cut(s)	1075
XspI	CTAG	2 cut(s)	143, 672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.