Rroxscaffold_2G00084230

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002135
Physical Location & Seq
Forward (+)
6716200 .. 6718305
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084230.1

Sequence Viewer

Length: 1026 bp
ATGTCAGACCTCCCCAACCCTCTCTACGATTTCATCATCCTCGGCGCCTCCGGCTTCACTGGAAAGCACGTCGTGAAAGAAAGCCTCAAGTTCCTCAACACCCCATCTTCACCTCTCAAGGACGGCCGCAACCCCGCAAAGCTCTCCCAAACTCTCAACTGGGCCACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACGCCACCGATCCTCGGTCCCTCCGCCGCCTCTGCTCCCAGACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAGCCCGTCGTTGCCGCGTGCGTTGAGACCGGCTGTGATTATCTCGACATATGCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAAGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTCGACTCTGTTCCGGCGGAGTTTGGGTTGATGTTTAACTCGAGGCAGTGGGTGGGGGCGGCAGTGCCGAGTAGGGTGGAGGCTTATGTGAGCTTGGAGTCCGAGAAGAGAATTGTTGGCAACTTCGGGACGTTTGAGTCGGCGGTGTTGGGTGTGGCTAATGCGGATAAGTTGAAGAAGCTCCGGCGGTCCAGACCTAAAAAGCCGAGGCCGGAGTTGAAAACTTTGCTGCTATGTTCTGTTTTTGGGATAGTAATGAAGCAGGGTCCCTCCGATGAGGAGGTGAGAAGTGCATCTTTTAAGATGTGGTTTGTTGGACGTGGTTTCAGCGACTGGAGTCTTGCTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAGTTGGCTACATAACAACCCCGATAATTCTACTTCACTGTGCACTTATTGTTCTGAGCCAATGCGACAATCTTCCAAAGGGAGGAGTTCTCCCACCTGGGATTGTATTTGGCTCAACTGATCTCCAAGAAAAGCTACAAGAAAATGGAGTTACTTTTGATGTTATTTCAAAGAATGTTATTTCTCATTAA

Protein Analysis

341

Amino Acids

37.18

Weight (kDa)

7.5

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 140 3.6e-10 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 311
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 855
AcyI GRCGYC 2 cut(s) 45, 210
AdeI CACNNNGTG 1 cut(s) 73
AfiI CCNNNNNNNGG 3 cut(s) 222, 839, 917
AgsI TTSAA 3 cut(s) 610, 655, 1005
AjiI CACGTC 2 cut(s) 70, 755
AjnI CCWGG 1 cut(s) 931
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 4 cut(s) 142, 528, 616, 970
AluI AGCT 4 cut(s) 142, 528, 616, 970
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 1 cut(s) 314
Alw44I GTGCAC 1 cut(s) 876
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 1 cut(s) 768
Ama87I CYCGRG 1 cut(s) 475
AoxI GGCC 6 cut(s) 124, 162, 169, 274, 378, 644
ApaI GGGCCC 1 cut(s) 278
ApaLI GTGCAC 1 cut(s) 876
ApeKI GCWGC 1 cut(s) 664
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 8 cut(s) 162, 170, 225, 274, 275, 624, 701, 830
AsuHPI GGTGA 3 cut(s) 102, 193, 730
AvaI CYCGRG 1 cut(s) 475
AvaII GGWCC 4 cut(s) 225, 624, 701, 830
BaeGI GKGCMC 2 cut(s) 278, 880
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 3 cut(s) 278, 300, 413
Bbv12I GWGCWC 1 cut(s) 880
BbvI GCAGC 1 cut(s) 651
BccI CCATC 3 cut(s) 112, 203, 367
BceAI ACGGC 2 cut(s) 139, 307
BciT130I CCWGG 1 cut(s) 933
BcoDI GTCTC 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 48
BglI GCCNNNNNGGC 1 cut(s) 291
BisI GCNGC 6 cut(s) 127, 235, 309, 399, 495, 665
BlsI GCNGC 6 cut(s) 128, 236, 310, 400, 496, 666
Bme1390I CCNGG 1 cut(s) 933
Bme18I GGWCC 4 cut(s) 225, 624, 701, 830
BmeT110I CYCGRG 1 cut(s) 475
BmgBI CACGTC 2 cut(s) 70, 755
BmgT120I GGNCC 8 cut(s) 162, 170, 225, 274, 275, 624, 701, 830
BmiI GGNNCC 7 cut(s) 46, 227, 276, 277, 702, 703, 831
BmrFI CCNGG 1 cut(s) 933
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 737
BmuI ACTGGG 1 cut(s) 169
BoxI GACNNNNGTC 1 cut(s) 771
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 909, 941
BpmI CTGGAG 1 cut(s) 790
BpuEI CTTGAG 2 cut(s) 71, 101
BsaHI GRCGYC 2 cut(s) 45, 210
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 40, 221, 641, 932
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 839, 917
Bse118I RCCGGY 2 cut(s) 167, 323
Bse1I ACTGG 4 cut(s) 64, 164, 381, 773
BseBI CCWGG 1 cut(s) 933
BseDI CCNNGG 4 cut(s) 40, 221, 641, 932
BseGI GGATG 4 cut(s) 36, 195, 258, 378
BseLI CCNNNNNNNGG 3 cut(s) 222, 839, 917
BseMII CTCAG 2 cut(s) 797, 881
BseNI ACTGG 4 cut(s) 64, 164, 381, 773
BseRI GAGGAG 2 cut(s) 728, 933
BseSI GKGCMC 2 cut(s) 278, 880
BseX3I CGGCCG 1 cut(s) 124
BseXI GCAGC 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 6 cut(s) 126, 164, 171, 276, 380, 646
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 880
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 7 cut(s) 51, 168, 324, 356, 449, 619, 647
BslFI GGGAC 4 cut(s) 211, 577, 687, 843
BslI CCNNNNNNNGG 3 cut(s) 222, 839, 917
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 4 cut(s) 211, 577, 687, 843
BsnI GGCC 6 cut(s) 126, 164, 171, 276, 380, 646
Bso31I GGTCTC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 475
Bsp120I GGGCCC 1 cut(s) 274
Bsp1286I GDGCHC 4 cut(s) 278, 300, 413, 880
Bsp143I GATC 2 cut(s) 217, 955
BspANI GGCC 6 cut(s) 126, 164, 171, 276, 380, 646
BspCNI CTCAG 2 cut(s) 796, 882
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 7 cut(s) 46, 227, 276, 277, 702, 703, 831
BspPI GGATC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 314
BsrFI RCCGGY 2 cut(s) 167, 323
BsrI ACTGG 4 cut(s) 64, 164, 381, 773
BssAI RCCGGY 2 cut(s) 167, 323
BssECI CCNNGG 4 cut(s) 40, 221, 641, 932
BssMI GATC 2 cut(s) 217, 955
BssNI GRCGYC 2 cut(s) 45, 210
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 1 cut(s) 875
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 2 cut(s) 45, 210
BstC8I GCNNGC 2 cut(s) 296, 313
BstDEI CTNAG 2 cut(s) 783, 890
BstF5I GGATG 4 cut(s) 36, 195, 258, 378
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 2 cut(s) 220, 958
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 2 cut(s) 217, 955
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 4 cut(s) 51, 240, 282, 291
BstNI CCWGG 1 cut(s) 933
BstPAI GACNNNNGTC 1 cut(s) 771
BstSCI CCNGG 1 cut(s) 931
BstSLI GKGCMC 2 cut(s) 278, 880
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 651
BstZI CGGCCG 1 cut(s) 124
BsuRI GGCC 6 cut(s) 126, 164, 171, 276, 380, 646
BtrI CACGTC 2 cut(s) 70, 755
BtsCI GGATG 4 cut(s) 36, 195, 258, 378
BtsI GCAGTG 2 cut(s) 488, 504
BtsIMutI CAGTG 4 cut(s) 57, 488, 504, 871
Cac8I GCNNGC 2 cut(s) 296, 313
CaiI CAGNNNCTG 1 cut(s) 768
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 2 cut(s) 167, 323
Cfr13I GGNCC 8 cut(s) 162, 170, 225, 274, 275, 624, 701, 830
CseI GACGC 1 cut(s) 218
CviAII CATG 1 cut(s) 364
DdeI CTNAG 2 cut(s) 783, 890
DinI GGCGCC 1 cut(s) 46
DpnI GATC 2 cut(s) 219, 957
DpnII GATC 2 cut(s) 217, 955
DraIII CACNNNGTG 1 cut(s) 73
DrdI GACNNNNNNGTC 1 cut(s) 571
DseDI GACNNNNNNGTC 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 124
EagI CGGCCG 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
EciI GGCGGA 4 cut(s) 221, 272, 416, 467
EclXI CGGCCG 1 cut(s) 124
Eco24I GRGCYC 3 cut(s) 278, 300, 413
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 4 cut(s) 225, 624, 701, 830
Eco52I CGGCCG 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 475
EcoO109I RGGNCCY 3 cut(s) 275, 701, 830
EcoRII CCWGG 1 cut(s) 931
EcoT38I GRGCYC 3 cut(s) 278, 300, 413
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 1 cut(s) 367
FaiI YATR 8 cut(s) 344, 346, 365, 522, 670, 806, 827, 848
FalI AAGNNNNNCTT 2 cut(s) 715, 747
FaqI GGGAC 4 cut(s) 211, 577, 687, 843
FatI CATG 1 cut(s) 363
FauI CCCGC 2 cut(s) 142, 260
FauNDI CATATG 1 cut(s) 344
Fnu4HI GCNGC 6 cut(s) 127, 235, 309, 399, 495, 665
FokI GGATG 4 cut(s) 23, 182, 245, 385
FriOI GRGCYC 3 cut(s) 278, 300, 413
Fsp4HI GCNGC 6 cut(s) 127, 235, 309, 399, 495, 665
GlaI GCGC 1 cut(s) 46
GluI GCNGC 6 cut(s) 127, 235, 309, 399, 495, 665
GsuI CTGGAG 1 cut(s) 790
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 6 cut(s) 126, 164, 171, 276, 380, 646
HapII CCGG 7 cut(s) 51, 168, 324, 356, 449, 619, 647
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 2 cut(s) 45, 210
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 4 cut(s) 440, 533, 572, 772
HpaII CCGG 7 cut(s) 51, 168, 324, 356, 449, 619, 647
HphI GGTGA 3 cut(s) 102, 193, 730
Hpy166II GTNNAC 1 cut(s) 878
Hpy188I TCNGA 4 cut(s) 7, 538, 709, 891
Hpy188III TCNNGA 4 cut(s) 73, 338, 562, 627
Hpy8I GTNNAC 1 cut(s) 878
Hpy99I CGWCG 3 cut(s) 74, 212, 305
HpyAV CCTTC 2 cut(s) 289, 400
HpyCH4III ACNGT 1 cut(s) 875
HpyCH4IV ACGT 3 cut(s) 69, 566, 754
HpyCH4V TGCA 2 cut(s) 728, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 240, 282, 291
HpyF3I CTNAG 2 cut(s) 783, 890
HpySE526I ACGT 3 cut(s) 69, 566, 754
Hsp92I GRCGYC 2 cut(s) 45, 210
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KflI GGGWCCC 1 cut(s) 701
Kzo9I GATC 2 cut(s) 217, 955
LmnI GCTCC 2 cut(s) 248, 621
Lsp1109I GCAGC 1 cut(s) 651
LweI GCATC 1 cut(s) 737
MaeII ACGT 3 cut(s) 69, 566, 754
MaeIII GTNAC 2 cut(s) 814, 985
MalI GATC 2 cut(s) 219, 957
MboI GATC 2 cut(s) 217, 955
MboII GAAGA 4 cut(s) 99, 553, 622, 899
MhlI GDGCHC 4 cut(s) 278, 300, 413, 880
MluCI AATT 2 cut(s) 546, 861
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 4 cut(s) 434, 542, 581, 781
MmeI TCCRAC 1 cut(s) 730
MseI TTAA 3 cut(s) 471, 735, 1024
MspI CCGG 7 cut(s) 51, 168, 324, 356, 449, 619, 647
MspR9I CCNGG 1 cut(s) 933
MvaI CCWGG 1 cut(s) 933
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 51, 240, 282, 291
NarI GGCGCC 1 cut(s) 45
NdeI CATATG 1 cut(s) 344
NdeII GATC 2 cut(s) 217, 955
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 7 cut(s) 46, 227, 276, 277, 702, 703, 831
NmeAIII GCCGAG 3 cut(s) 21, 528, 666
PaeR7I CTCGAG 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PkrI GCNGC 6 cut(s) 128, 236, 310, 400, 496, 666
PleI GAGTC 4 cut(s) 434, 541, 580, 780
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 4 cut(s) 434, 541, 580, 780
PpuMI RGGWCCY 2 cut(s) 701, 830
PshAI GACNNNNGTC 1 cut(s) 771
Psp5II RGGWCCY 2 cut(s) 701, 830
Psp6I CCWGG 1 cut(s) 931
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 7 cut(s) 46, 227, 276, 277, 702, 703, 831
PspOMI GGGCCC 1 cut(s) 274
PspPI GGNCC 8 cut(s) 162, 170, 225, 274, 275, 624, 701, 830
PspPPI RGGWCCY 2 cut(s) 701, 830
PspXI VCTCGAGB 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 768
SaqAI TTAA 3 cut(s) 471, 735, 1024
SatI GCNGC 6 cut(s) 127, 235, 309, 399, 495, 665
Sau3AI GATC 2 cut(s) 217, 955
Sau96I GGNCC 8 cut(s) 162, 170, 225, 274, 275, 624, 701, 830
SchI GAGTC 4 cut(s) 434, 542, 581, 781
ScrFI CCNGG 1 cut(s) 933
SduI GDGCHC 4 cut(s) 278, 300, 413, 880
SfaNI GCATC 1 cut(s) 737
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 475
SinI GGWCC 4 cut(s) 225, 624, 701, 830
SlaI CTCGAG 1 cut(s) 475
SmlI CTYRAG 3 cut(s) 86, 116, 475
SmoI CTYRAG 3 cut(s) 86, 116, 475
Sse9I AATT 2 cut(s) 546, 861
SspDI GGCGCC 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 931
TaaI ACNGT 1 cut(s) 875
TaiI ACGT 3 cut(s) 72, 569, 757
TaqI TCGA 4 cut(s) 339, 390, 438, 476
TaqII GACCGA 1 cut(s) 213
TasI AATT 2 cut(s) 546, 861
TauI GCSGC 5 cut(s) 129, 237, 311, 401, 497
Tru1I TTAA 3 cut(s) 471, 735, 1024
Tru9I TTAA 3 cut(s) 471, 735, 1024
TscAI CASTG 4 cut(s) 64, 488, 504, 878
TseI GCWGC 1 cut(s) 664
TspDTI ATGAA 3 cut(s) 22, 352, 707
TspRI CASTG 4 cut(s) 64, 488, 504, 878
VneI GTGCAC 1 cut(s) 876
VpaK11BI GGWCC 4 cut(s) 225, 624, 701, 830
XhoI CTCGAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.