AT5G50400

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001351
Physical Location & Seq
Reverse (-)
20523366 .. 20526521
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50400.1

Sequence Viewer

Length: 1836 bp
ATGGCTAGGAATTTCTTGTTAGTGCTTCTATGGTTCATCGTACAGGTGTCTTCAAGTCACGAAAATGGACGTGGAGATCAAGCACTGTCTCAAATTGATATATACGCCATAAATTTAGCTCAGCATCACTCTGCTTTTATTCACGTTTCCCCTCTTGTTCTTGGTTCCCAGGGTCAGGACACAGAATGGGTAAATGTTGTTATCTCAAATCCGGAGCCTTCTTCAGACGATTGGGTTGGAGTCTTCTCACCAGCAAAATTCGACTCATCTTCTTGTGCTCCAACAGATGACAAAGAGATAGCTCCTTTTATTTGCTCAGCTCCAGTCAAGTATATGTATGCCAAGTCCAGTCCTGACTATATGAAGACTGGGAATGCGGTTTTGAAGTTCATGCTTATAAACCAGAGAGCTGATTTCTCCTTTGCCCTCTTCACTGGGGGACTCTCAAATCCAACACTTGTCTCGGTTTCAAATCATGTATCGTTTATCAATCCTAAAGCTCCTGTCTATCCACGTCTTGCTTTAGGGAAGAAATGGGACGAGATGACCGTAACATGGACAAGTGGTTACAACATAGGTGAGGCTGTTCCGTTTGTGGAATGGAGTCGTAAGGGAACTCGAAGTAGACGATCGCCAGCTGGTACCTTGACTTTTACCAGGAACAGCATGTGTGGTGCTCCTGCTCGTACTGTTGGTTGGCGTGATCCAGGTTTCATCCACACTGCTTCATTGAAAGATCTTTGGCCAAACCTCAAGTACACATACAGGATGGGTCATGAGTTGATGAATGGATCAATAGTTTGGAGCAAGAATTTCACTTTCAAGTCCTCTCCTTATCCTGGACAAGACTCGTTACAACGGGTCATAATCTTCGGGGACATGGGGAAGGGAGAGCGGGATGGTTCAAACGAGTACAATGATTATCAGCCTGGTTCTCTCAACACCACTGATCAACTCATCAAGGACTTGAAGAACATTGACATTGTTTTCCACATTGGAGATATCACTTACGCAAATGGTTACATCTCGCAGTGGGATCAGTTCACAGCTCAAGTTGAACCTATTGCTTCTACTGTTCCATACATGGTCGCAAGTGGCAACCACGAGCGGGATTGGCCAGACTCTGGATCCTTCTATGGTGGTAAAGACTCTGGAGGGGAGTGTGGGGTTCCTGCGGAAACCATGTTTGATTTTCCTGCTGAGAACAAAGCTAAGTTCTGGTACTCTGCGGATTATGGAATGTTTCGGTTCTGTGTAGCGGACACTGAACATGATTGGAGAGAAGGGTCAGAGCAATACCAATTCATAGAGCGTTGTCTTGCCTCTGTAGACAGAAGGGCTCAGCCTTGGTTGATTTTTATTGCTCATCGTGTTCTCGGTTATTCAACAAACGATTGGTATGGTCAAGAAGGTTCCTTTGAAGAACCTATGGGAAGAGAGAGCTTGCAAAAGCTCTGGCAGAAGTATAAAGTAGACATCGCGTTCTACGGTCATGTTCATAACTATGAAAGAACATGCCCCATTTACCAGAACCAATGTATGGACAATGAGAAAAGTCACTACTCAGGAGCCTTCAAAGGAACTATACACGTGGTTGTTGGTGGTGCAGGAAGTCACTTGTCTTCGTTCAGCTCGCTGAAACCCAAGTGGAGTATCTTCAGAGACTATGATTACGGGTTTGTGAAGTTGACGGCTTTTGATCACTCCTCTCTTCTCTTCGAATACAAGAAAAGCAGCAATGGAGCTGTACATGACTCCTTCACTATCTTTAGAGAATACAGAGATGTCTTGGCTTGTGTTCGAGATAGCTGCGAGCCAACCACATTAGCTTCATGA

Protein Analysis

611

Amino Acids

68.9

Weight (kDa)

5.99

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 47 - 164 7.2e-48 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 169 - 275 6e-14 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 287 - 502 1.1e-16 Calcineurin-like phosphoesterase
Metallophos_C PF14008 527 - 586 2.8e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 641
AccB7I CCANNNNNTGG 2 cut(s) 1124, 1540
AccBSI CCGCTC 2 cut(s) 895, 1108
AccI GTMKAC 3 cut(s) 625, 1329, 1473
AccII CGCG 1 cut(s) 1481
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 6 cut(s) 377, 895, 1108, 1175, 1229, 1259
AclWI GGATC 5 cut(s) 698, 799, 1044, 1122, 1135
AcoI YGGCCR 2 cut(s) 743, 1115
AcsI RAATTY 4 cut(s) 10, 112, 257, 811
AcuI CTGAAG 2 cut(s) 207, 1642
AcvI CACGTG 1 cut(s) 1591
AfaI GTAC 7 cut(s) 42, 643, 688, 758, 914, 1223, 1749
AfiI CCNNNNNNNGG 6 cut(s) 175, 555, 839, 1108, 1124, 1540
AflIII ACRYGT 1 cut(s) 1588
AjiI CACGTC 2 cut(s) 71, 515
AjnI CCWGG 5 cut(s) 168, 656, 706, 838, 928
Alw21I GWGCWC 2 cut(s) 280, 679
Alw26I GTCTC 3 cut(s) 93, 466, 1656
AlwI GGATC 5 cut(s) 698, 799, 1044, 1122, 1135
AlwNI CAGNNNCTG 1 cut(s) 1124
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 2 cut(s) 743, 1115
ApeKI GCWGC 2 cut(s) 1734, 1809
ApoI RAATTY 4 cut(s) 10, 112, 257, 811
Asp718I GGTACC 1 cut(s) 641
AsuHPI GGTGA 2 cut(s) 240, 590
AsuII TTCGAA 1 cut(s) 1719
BalI TGGCCA 2 cut(s) 745, 1117
BamHI GGATCC 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 1342
BauI CACGAG 1 cut(s) 1103
BbrPI CACGTG 1 cut(s) 1591
BbsI GAAGAC 4 cut(s) 42, 235, 371, 1614
Bbv12I GWGCWC 2 cut(s) 280, 679
BbvI GCAGC 2 cut(s) 1746, 1796
BccI CCATC 2 cut(s) 763, 893
BceAI ACGGC 1 cut(s) 1707
BcgI CGANNNNNNTGC 2 cut(s) 1791, 1825
BciT130I CCWGG 5 cut(s) 170, 658, 708, 840, 930
BclI TGATCA 2 cut(s) 949, 1699
BcoDI GTCTC 3 cut(s) 93, 466, 1656
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 1326
BglII AGATCT 1 cut(s) 736
BisI GCNGC 2 cut(s) 1735, 1810
BlpI GCTNAGC 3 cut(s) 120, 316, 1341
BlsI GCNGC 2 cut(s) 1736, 1811
Bme1390I CCNGG 5 cut(s) 170, 658, 708, 840, 930
BmgBI CACGTC 2 cut(s) 71, 515
BmiI GGNNCC 7 cut(s) 166, 216, 643, 1129, 1170, 1414, 1570
BmrFI CCNGG 5 cut(s) 170, 658, 708, 840, 930
BmrI ACTGGG 2 cut(s) 378, 444
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 2 cut(s) 378, 444
BpiI GAAGAC 4 cut(s) 42, 235, 371, 1614
BpmI CTGGAG 2 cut(s) 306, 1173
Bpu1102I GCTNAGC 3 cut(s) 120, 316, 1341
Bpu14I TTCGAA 1 cut(s) 1719
BpuEI CTTGAG 2 cut(s) 737, 1035
BsaAI YACGTR 1 cut(s) 1591
BsaJI CCNNGG 3 cut(s) 168, 169, 1346
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 6 cut(s) 175, 555, 839, 1108, 1124, 1540
Bse1I ACTGG 4 cut(s) 323, 348, 373, 439
Bse3DI GCAATG 1 cut(s) 1744
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 5 cut(s) 170, 658, 708, 840, 930
BseDI CCNNGG 3 cut(s) 168, 169, 1346
BseGI GGATG 3 cut(s) 714, 774, 904
BseLI CCNNNNNNNGG 6 cut(s) 175, 555, 839, 1108, 1124, 1540
BseMI GCAATG 1 cut(s) 1744
BseMII CTCAG 5 cut(s) 134, 330, 1191, 1355, 1578
BseNI ACTGG 4 cut(s) 323, 348, 373, 439
BseRI GAGGAG 1 cut(s) 1696
BseXI GCAGC 2 cut(s) 1746, 1796
BsgI GTGCAG 1 cut(s) 1626
Bsh1236I CGCG 1 cut(s) 1481
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 2 cut(s) 745, 1117
BshNI GGYRCC 1 cut(s) 641
BsiEI CGRYCG 1 cut(s) 632
BsiHKAI GWGCWC 2 cut(s) 280, 679
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 3 cut(s) 453, 551, 890
BslI CCNNNNNNNGG 6 cut(s) 175, 555, 839, 1108, 1124, 1540
BsmAI GTCTC 3 cut(s) 93, 466, 1656
BsmFI GGGAC 3 cut(s) 453, 551, 890
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 2 cut(s) 745, 1117
Bsp119I TTCGAA 1 cut(s) 1719
Bsp1286I GDGCHC 3 cut(s) 280, 679, 1342
Bsp13I TCCGGA 1 cut(s) 211
Bsp1407I TGTACA 1 cut(s) 1747
Bsp143I GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
Bsp1720I GCTNAGC 3 cut(s) 120, 316, 1341
BspACI CCGC 6 cut(s) 377, 895, 1108, 1175, 1229, 1259
BspANI GGCC 2 cut(s) 745, 1117
BspCNI CTCAG 5 cut(s) 133, 329, 1192, 1354, 1577
BspEI TCCGGA 1 cut(s) 211
BspFNI CGCG 1 cut(s) 1481
BspHI TCATGA 2 cut(s) 775, 1832
BspLI GGNNCC 7 cut(s) 166, 216, 643, 1129, 1170, 1414, 1570
BspPI GGATC 5 cut(s) 698, 799, 1044, 1122, 1135
BspT104I TTCGAA 1 cut(s) 1719
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 2 cut(s) 895, 1108
BsrDI GCAATG 1 cut(s) 1744
BsrGI TGTACA 1 cut(s) 1747
BsrI ACTGG 4 cut(s) 323, 348, 373, 439
BssECI CCNNGG 3 cut(s) 168, 169, 1346
BssMI GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
BssSI CACGAG 1 cut(s) 1103
BssT1I CCWWGG 1 cut(s) 1346
Bst2BI CACGAG 1 cut(s) 1103
Bst2UI CCWGG 5 cut(s) 170, 658, 708, 840, 930
Bst4CI ACNGT 5 cut(s) 87, 550, 691, 1075, 1490
Bst6I CTCTTC 4 cut(s) 434, 1429, 1716, 1721
BstAUI TGTACA 1 cut(s) 1747
BstBAI YACGTR 1 cut(s) 1591
BstBI TTCGAA 1 cut(s) 1719
BstC8I GCNNGC 4 cut(s) 636, 1445, 1634, 1814
BstDEI CTNAG 6 cut(s) 120, 316, 1200, 1212, 1341, 1564
BstF5I GGATG 3 cut(s) 714, 774, 904
BstFNI CGCG 1 cut(s) 1481
BstKTI GATC 9 cut(s) 79, 632, 706, 739, 794, 952, 1039, 1130, 1702
BstMAI GTCTC 3 cut(s) 93, 466, 1656
BstMBI GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
BstMCI CGRYCG 1 cut(s) 632
BstMWI GCNNNNNNNGC 1 cut(s) 1114
BstNI CCWGG 5 cut(s) 170, 658, 708, 840, 930
BstNSI RCATGY 2 cut(s) 670, 1518
BstSCI CCNGG 5 cut(s) 168, 656, 706, 838, 928
BstSFI CTRYAG 1 cut(s) 1326
BstUI CGCG 1 cut(s) 1481
BstV1I GCAGC 2 cut(s) 1746, 1796
BstV2I GAAGAC 4 cut(s) 42, 235, 371, 1614
BstX2I RGATCY 2 cut(s) 736, 1127
BstYI RGATCY 2 cut(s) 736, 1127
BsuRI GGCC 2 cut(s) 745, 1117
BtgZI GCGATG 1 cut(s) 1462
BtrI CACGTC 2 cut(s) 71, 515
BtsCI GGATG 3 cut(s) 714, 774, 904
BtsI GCAGTG 2 cut(s) 720, 1037
BtsIMutI CAGTG 6 cut(s) 83, 432, 720, 945, 1037, 1263
Cac8I GCNNGC 4 cut(s) 636, 1445, 1634, 1814
CaiI CAGNNNCTG 1 cut(s) 1124
CciI TCATGA 2 cut(s) 775, 1832
Csp6I GTAC 7 cut(s) 41, 642, 687, 757, 913, 1222, 1748
CviQI GTAC 7 cut(s) 41, 642, 687, 757, 913, 1222, 1748
DdeI CTNAG 6 cut(s) 120, 316, 1200, 1212, 1341, 1564
DpnI GATC 9 cut(s) 78, 631, 705, 738, 793, 951, 1038, 1129, 1701
DpnII GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
EaeI YGGCCR 2 cut(s) 743, 1115
Eam1104I CTCTTC 4 cut(s) 434, 1429, 1716, 1721
EarI CTCTTC 4 cut(s) 434, 1429, 1716, 1721
Eco130I CCWWGG 1 cut(s) 1346
Eco24I GRGCYC 1 cut(s) 1342
Eco32I GATATC 1 cut(s) 1003
Eco57I CTGAAG 2 cut(s) 207, 1642
Eco72I CACGTG 1 cut(s) 1591
EcoRII CCWGG 5 cut(s) 168, 656, 706, 838, 928
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 1346
EcoT38I GRGCYC 1 cut(s) 1342
ErhI CCWWGG 1 cut(s) 1346
FaqI GGGAC 3 cut(s) 453, 551, 890
FauI CCCGC 2 cut(s) 888, 1101
FbaI TGATCA 2 cut(s) 949, 1699
FblI GTMKAC 3 cut(s) 625, 1329, 1473
Fnu4HI GCNGC 2 cut(s) 1735, 1810
FokI GGATG 3 cut(s) 701, 781, 911
FriOI GRGCYC 1 cut(s) 1342
Fsp4HI GCNGC 2 cut(s) 1735, 1810
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 1735, 1810
GsuI CTGGAG 2 cut(s) 306, 1173
HaeIII GGCC 2 cut(s) 745, 1117
HapII CCGG 1 cut(s) 212
HincII GTYRAC 1 cut(s) 1689
HindII GTYRAC 1 cut(s) 1689
HinfI GANTC 8 cut(s) 240, 263, 441, 604, 848, 1121, 1148, 1754
HpaII CCGG 1 cut(s) 212
HphI GGTGA 2 cut(s) 240, 590
Hpy166II GTNNAC 6 cut(s) 626, 759, 1044, 1330, 1474, 1689
Hpy188I TCNGA 3 cut(s) 226, 1291, 1661
Hpy8I GTNNAC 6 cut(s) 626, 759, 1044, 1330, 1474, 1689
HpyAV CCTTC 8 cut(s) 228, 880, 1141, 1277, 1329, 1403, 1582, 1768
HpyCH4III ACNGT 5 cut(s) 87, 550, 691, 1075, 1490
HpyCH4IV ACGT 4 cut(s) 70, 144, 514, 1590
HpyCH4V TGCA 2 cut(s) 1447, 1607
HpyF10VI GCNNNNNNNGC 1 cut(s) 1114
HpyF3I CTNAG 6 cut(s) 120, 316, 1200, 1212, 1341, 1564
HpySE526I ACGT 4 cut(s) 70, 144, 514, 1590
Kpn2I TCCGGA 1 cut(s) 211
KpnI GGTACC 1 cut(s) 645
Ksp22I TGATCA 2 cut(s) 949, 1699
Kzo9I GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
LmnI GCTCC 9 cut(s) 214, 283, 307, 325, 505, 682, 804, 1568, 1742
Lsp1109I GCAGC 2 cut(s) 1746, 1796
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 6
MaeII ACGT 4 cut(s) 70, 144, 514, 1590
MaeIII GTNAC 7 cut(s) 56, 550, 566, 852, 1019, 1556, 1613
MalI GATC 9 cut(s) 78, 631, 705, 738, 793, 951, 1038, 1129, 1701
MbiI CCGCTC 2 cut(s) 895, 1108
MboI GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
MflI RGATCY 2 cut(s) 736, 1127
MhlI GDGCHC 3 cut(s) 280, 679, 1342
MlsI TGGCCA 2 cut(s) 745, 1117
MluCI AATT 6 cut(s) 10, 93, 112, 257, 811, 1301
MluNI TGGCCA 2 cut(s) 745, 1117
MlyI GAGTC 8 cut(s) 249, 257, 435, 613, 842, 1115, 1142, 1748
MmeI TCCRAC 3 cut(s) 217, 305, 476
MnlI CCTC 8 cut(s) 162, 437, 574, 761, 838, 1148, 1333, 1717
Mox20I TGGCCA 2 cut(s) 745, 1117
MroI TCCGGA 1 cut(s) 211
MscI TGGCCA 2 cut(s) 745, 1117
MslI CAYNNNNRTG 2 cut(s) 63, 1503
Msp20I TGGCCA 2 cut(s) 745, 1117
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 5 cut(s) 170, 658, 708, 840, 930
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 5 cut(s) 170, 658, 708, 840, 930
MvnI CGCG 1 cut(s) 1481
MwoI GCNNNNNNNGC 1 cut(s) 1114
NdeII GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
NlaIV GGNNCC 7 cut(s) 166, 216, 643, 1129, 1170, 1414, 1570
NmuCI GTSAC 3 cut(s) 56, 1556, 1613
NspI RCATGY 2 cut(s) 670, 1518
NspV TTCGAA 1 cut(s) 1719
PagI TCATGA 2 cut(s) 775, 1832
PasI CCCWGGG 1 cut(s) 169
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 625
PctI GAATGC 1 cut(s) 379
PflMI CCANNNNNTGG 2 cut(s) 1124, 1540
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 2 cut(s) 1736, 1811
Ple19I CGATCG 1 cut(s) 632
PleI GAGTC 8 cut(s) 248, 257, 435, 612, 842, 1115, 1142, 1748
PmaCI CACGTG 1 cut(s) 1591
PmlI CACGTG 1 cut(s) 1591
PpsI GAGTC 8 cut(s) 248, 257, 435, 612, 842, 1115, 1142, 1748
Ppu21I YACGTR 1 cut(s) 1591
PsiI TTATAA 1 cut(s) 398
Psp6I CCWGG 5 cut(s) 168, 656, 706, 838, 928
PspCI CACGTG 1 cut(s) 1591
PspGI CCWGG 5 cut(s) 168, 656, 706, 838, 928
PspN4I GGNNCC 7 cut(s) 166, 216, 643, 1129, 1170, 1414, 1570
PstNI CAGNNNCTG 1 cut(s) 1124
PsuI RGATCY 2 cut(s) 736, 1127
PvuI CGATCG 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 7 cut(s) 42, 643, 688, 758, 914, 1223, 1749
RsaNI GTAC 7 cut(s) 41, 642, 687, 757, 913, 1222, 1748
RseI CAYNNNNRTG 2 cut(s) 63, 1503
SatI GCNGC 2 cut(s) 1735, 1810
Sau3AI GATC 9 cut(s) 76, 629, 703, 736, 791, 949, 1036, 1127, 1699
SchI GAGTC 8 cut(s) 249, 257, 435, 613, 842, 1115, 1142, 1748
ScrFI CCNGG 5 cut(s) 170, 658, 708, 840, 930
SduI GDGCHC 3 cut(s) 280, 679, 1342
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 1326
SfuI TTCGAA 1 cut(s) 1719
SmiMI CAYNNNNRTG 2 cut(s) 63, 1503
SmlI CTYRAG 2 cut(s) 752, 1050
SmoI CTYRAG 2 cut(s) 752, 1050
Sse9I AATT 6 cut(s) 10, 93, 112, 257, 811, 1301
SsiI CCGC 6 cut(s) 377, 895, 1108, 1175, 1229, 1259
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 5 cut(s) 168, 656, 706, 838, 928
StyI CCWWGG 1 cut(s) 1346
TaaI ACNGT 5 cut(s) 87, 550, 691, 1075, 1490
TaiI ACGT 4 cut(s) 73, 147, 517, 1593
TaqI TCGA 4 cut(s) 261, 619, 1719, 1801
TasI AATT 6 cut(s) 10, 93, 112, 257, 811, 1301
TatI WGTACW 3 cut(s) 756, 912, 1747
TscAI CASTG 6 cut(s) 90, 439, 727, 952, 1037, 1270
TseFI GTSAC 3 cut(s) 56, 1556, 1613
TseI GCWGC 2 cut(s) 1734, 1809
Tsp45I GTSAC 3 cut(s) 56, 1556, 1613
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 6 cut(s) 90, 439, 727, 952, 1037, 1270
Van91I CCANNNNNTGG 2 cut(s) 1124, 1540
XapI RAATTY 4 cut(s) 10, 112, 257, 811
XceI RCATGY 2 cut(s) 670, 1518
XmiI GTMKAC 3 cut(s) 625, 1329, 1473
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.