MD06G1142200.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001351
Physical Location & Seq
Forward (+)
28584454 .. 28587742
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1142200.v1.1.491

Sequence Viewer

Length: 1914 bp
ATGGCTGAAACATCAAGGCTTAAACACTTGGGGTCTCAGAAATTTGGACAGATGGAGGGCTACAGCTTCTTGTCCGTCAAAGTTGTGTTGGTGTTGGTTTTGCTGGTGCAGTACACAGCAGCTGATCACAGTCACAAGAAGCACGCCGGCGACGGGGTGCAGCCGCTGTCCAAGATTCAAATCCACCGAGCTGTTTACGAGCTTCACGAAAATGCTTCCGTCAAAGCTTACCCTGTCCTTCTTGGCACCAAAGGGGAAGATTCTCAGTGGGTAACTATTGAAGTTGCAAGTCCGAAGCCTGCTGAAGATGATTGGTTGGCAGTCTTTTCTCCTGCAGATTTCAATTCATCGACTTGTCCACCTACCGACGATAGAGAAGTGGCTCCAAATATATGCTCAGCTCCGATAAAGTACAGATTTGCCAATGATTCCAATGCTGGCTATACGAAAACAGGCAAAGCTTCTTTGAAGTTTCAGTTGATCAATCAACGGGCGGATTTTTCATTCGCATTATTCTCAGGCGGGCTTTCGACCCCAAAACTGGTGGCAGTTTCTAATTCTGTAAGTTTTGTTAATCCGAATGTACCCCTTTACCCGCGTCTTGCTCAAGGGAAGGACTGGAATGAAATGACAGTAACCTGGACAAGCGGCTACAACATCAACAAAGCTGTACCTTTTGTCGAGTGGGGTCTCAAGGGCGAAGCTCAAATTCGATCTCCAGCAGGAACACTGACTTTCCCTCGAGGAAGCTTGTGTGGTCCACCAGCGCGAACAGTTGGTTGGCGGGATCCTGGTTTCATCCATACAAGTTTTCTAAAAGATTTGTGGCCAAACTCATTGTACACTTACAAGCTCGGTCATAAATTATCTAATGGTTCCTATATCTGGAGCAAGTCCTACCACTTCAAGTCATCACCATACCCGGGGGAGGACTCATTACAGCGCTTAGTTATATTTGGCGACATGGGAAAGGCTGAGCGTGATAGTTCAAATGAATACGCTGATTATCAGCCTGGCTCACTCAATACAACAGATACACTGATAAAGGACTTAGATAACATTGACATAGTATTTCACATCGGAGATATTGTCTATGCCAATGGATACATCTCACAGTGGGATCAGTTCACGTCACAGGTGGAGCCGATTGCATCAAATGTGCCATATATGATTGCAAGTGGCAATCACGAACGCGATGCACCAGGGACAGGATCCTTCTATGACGGCAATGATTCAGGCGGCGAATGTGGTGTCCTGGCGGAGACTATGTTTTATGTTCCTGCTGAGAACAGAGCGAAGTTCTGGTATTCGACAGATTATGGGATGTTCCACTTTTGCATAGCTGACAGTGAGCACGATTGGAGGGAAGGCTCTGAGCAGTACAAATTCATTGAGAAATGCCTTGCGTCCGCGGACAGGCAGAAACAGCCTTGGCTGATATTTGCTGCTCATCGTGTTCTTGGTTATTCGTCCGCACAGTGGCAAGATGGCGCATTTGGCGAGCCTATGGGAAGGGAAAGCTTGCAAAAGCTTTGGCAAAAGTACAAGGTGGACATTGCATTTTACGGTCATGTCCATAACTACGAAAGAACATGCCCCATTTACCAGAATCAGTGTGTGAATACAGAAAAGTCTCATTACTCAGGAACTGTGAATGGAACTATACATGTTGTTGTTGGTGGAGGAGGCAGCCACTTATCGGCTTTTGGCCCGGTGCAAACGAGTTGGAGCCTTTTCAGAGATTCCGACTTTGGCTTCGTAAAACTTACGGCATTTAACCACTCGTCGCTTCTGTTTGAGTACAAGAAGAGCAATGATGGGAAGGTGTATGATTCCTTCACTATCTCAAGGGATTATAAAGATGTTTTGGCTTGTGTTCATGATGGCTGTGAACCAACTACTTTAGCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

638

Amino Acids

70.97

Weight (kDa)

6.09

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 74 - 192 7e-47 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 199 - 303 6.3e-11 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 315 - 528 8.4e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 553 - 612 1.7e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1857
AbsI CCTCGAGG 1 cut(s) 741
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 4 cut(s) 598, 769, 1194, 1412
AclWI GGATC 5 cut(s) 782, 795, 1128, 1206, 1219
AcoI YGGCCR 1 cut(s) 827
AcsI RAATTY 3 cut(s) 41, 708, 1385
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 8 cut(s) 113, 413, 585, 672, 842, 1382, 1544, 1802
AfeI AGCGCT 1 cut(s) 944
AfiI CCNNNNNNNGG 9 cut(s) 153, 541, 885, 923, 928, 1208, 1416, 1479, 1699
AflIII ACRYGT 1 cut(s) 1666
AgsI TTSAA 6 cut(s) 179, 281, 343, 469, 907, 990
AjiI CACGTC 1 cut(s) 1131
AjnI CCWGG 5 cut(s) 638, 790, 1012, 1201, 1254
AjuI GAANNNNNNNTTGG 2 cut(s) 763, 795
Alw21I GWGCWC 1 cut(s) 1356
Alw26I GTCTC 4 cut(s) 39, 695, 1256, 1638
AlwI GGATC 5 cut(s) 782, 795, 1128, 1206, 1219
AlwNI CAGNNNCTG 3 cut(s) 122, 166, 1649
Ama87I CYCGRG 2 cut(s) 741, 922
Aor51HI AGCGCT 1 cut(s) 944
AoxI GGCC 2 cut(s) 827, 1708
ApeKI GCWGC 4 cut(s) 119, 160, 1445, 1689
ApoI RAATTY 3 cut(s) 41, 708, 1385
ArsI GACNNNNNNTTYG 4 cut(s) 1236, 1268, 1739, 1771
AspLEI GCGC 3 cut(s) 769, 945, 1493
AspS9I GGNCC 2 cut(s) 758, 1709
AsuC2I CCSGG 3 cut(s) 923, 924, 1712
AsuHPI GGTGA 1 cut(s) 906
AvaI CYCGRG 2 cut(s) 741, 922
AvaII GGWCC 1 cut(s) 758
BalI TGGCCA 1 cut(s) 829
BamHI GGATCC 2 cut(s) 787, 1211
BanI GGYRCC 1 cut(s) 245
BarI GAAGNNNNNNTAC 2 cut(s) 1820, 1852
Bbv12I GWGCWC 1 cut(s) 1356
BbvI GCAGC 4 cut(s) 131, 172, 1432, 1701
BccI CCATC 4 cut(s) 46, 1481, 1811, 1877
BceAI ACGGC 2 cut(s) 1240, 1785
BcgI CGANNNNNNTGC 2 cut(s) 1178, 1212
BciT130I CCWGG 5 cut(s) 640, 792, 1014, 1203, 1256
BciVI GTATCC 1 cut(s) 1097
BclI TGATCA 2 cut(s) 124, 480
BcnI CCSGG 3 cut(s) 923, 924, 1712
BcoDI GTCTC 4 cut(s) 39, 695, 1256, 1638
BfmI CTRYAG 2 cut(s) 61, 333
BfoI RGCGCY 1 cut(s) 946
BfuI GTATCC 1 cut(s) 1097
BisI GCNGC 7 cut(s) 120, 161, 164, 649, 1240, 1446, 1690
BlpI GCTNAGC 2 cut(s) 397, 975
BlsI GCNGC 7 cut(s) 121, 162, 165, 650, 1241, 1447, 1691
Bme1390I CCNGG 8 cut(s) 640, 792, 923, 924, 1014, 1203, 1256, 1712
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 2 cut(s) 741, 922
BmgBI CACGTC 1 cut(s) 1131
BmgT120I GGNCC 2 cut(s) 758, 1709
BmiI GGNNCC 7 cut(s) 247, 384, 789, 877, 1143, 1213, 1730
BmrFI CCNGG 8 cut(s) 640, 792, 923, 924, 1014, 1203, 1256, 1712
BmsI GCATC 2 cut(s) 1160, 1186
BpmI CTGGAG 2 cut(s) 702, 907
Bpu1102I GCTNAGC 2 cut(s) 397, 975
BpuEI CTTGAG 3 cut(s) 591, 677, 1831
BpuMI CCSGG 3 cut(s) 923, 924, 1712
BsaBI GATNNNNATC 1 cut(s) 179
BsaI GGTCTC 2 cut(s) 39, 695
BsaJI CCNNGG 5 cut(s) 922, 923, 1202, 1410, 1430
BsaXI ACNNNNNCTCC 4 cut(s) 1074, 1104, 1253, 1283
Bsc4I CCNNNNNNNGG 9 cut(s) 153, 541, 885, 923, 928, 1208, 1416, 1479, 1699
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 546, 623
Bse3DI GCAATG 3 cut(s) 1234, 1554, 1819
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 5 cut(s) 640, 792, 1014, 1203, 1256
BseDI CCNNGG 5 cut(s) 922, 923, 1202, 1410, 1430
BseGI GGATG 2 cut(s) 798, 1329
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 9 cut(s) 153, 541, 885, 923, 928, 1208, 1416, 1479, 1699
BseMI GCAATG 3 cut(s) 1234, 1554, 1819
BseMII CTCAG 8 cut(s) 50, 278, 411, 531, 966, 1275, 1365, 1656
BseNI ACTGG 2 cut(s) 546, 623
BseRI GAGGAG 1 cut(s) 1698
BseXI GCAGC 4 cut(s) 131, 172, 1432, 1701
BsgI GTGCAG 2 cut(s) 128, 179
Bsh1236I CGCG 4 cut(s) 598, 769, 1194, 1412
BshFI GGCC 2 cut(s) 829, 1710
BshNI GGYRCC 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 1356
BsiHKCI CYCGRG 2 cut(s) 741, 922
BsiSI CCGG 3 cut(s) 147, 923, 1712
BslFI GGGAC 1 cut(s) 1219
BslI CCNNNNNNNGG 9 cut(s) 153, 541, 885, 923, 928, 1208, 1416, 1479, 1699
BsmAI GTCTC 4 cut(s) 39, 695, 1256, 1638
BsmFI GGGAC 1 cut(s) 1219
BsnI GGCC 2 cut(s) 829, 1710
Bso31I GGTCTC 2 cut(s) 39, 695
BsoBI CYCGRG 2 cut(s) 741, 922
Bsp1286I GDGCHC 1 cut(s) 1356
Bsp1407I TGTACA 1 cut(s) 840
Bsp143I GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
Bsp1720I GCTNAGC 2 cut(s) 397, 975
BspANI GGCC 2 cut(s) 829, 1710
BspCNI CTCAG 8 cut(s) 49, 277, 410, 530, 967, 1276, 1366, 1655
BspFNI CGCG 4 cut(s) 598, 769, 1194, 1412
BspHI TCATGA 1 cut(s) 1879
BspLI GGNNCC 7 cut(s) 247, 384, 789, 877, 1143, 1213, 1730
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 5 cut(s) 782, 795, 1128, 1206, 1219
BspQI GCTCTTC 1 cut(s) 1802
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 2 cut(s) 39, 695
BsrDI GCAATG 3 cut(s) 1234, 1554, 1819
BsrFI RCCGGY 1 cut(s) 146
BsrGI TGTACA 1 cut(s) 840
BsrI ACTGG 2 cut(s) 546, 623
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 5 cut(s) 922, 923, 1202, 1410, 1430
BssMI GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
BssT1I CCWWGG 1 cut(s) 1430
Bst2UI CCWGG 5 cut(s) 640, 792, 1014, 1203, 1256
Bst4CI ACNGT 8 cut(s) 131, 634, 775, 1116, 1349, 1479, 1568, 1651
Bst6I CTCTTC 1 cut(s) 1802
BstAUI TGTACA 1 cut(s) 840
BstC8I GCNNGC 7 cut(s) 144, 148, 300, 439, 524, 1502, 1523
BstDSI CCRYGG 1 cut(s) 1410
BstF5I GGATG 2 cut(s) 798, 1329
BstFNI CGCG 4 cut(s) 598, 769, 1194, 1412
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 3 cut(s) 769, 945, 1493
BstKTI GATC 6 cut(s) 127, 483, 716, 790, 1123, 1214
BstMAI GTCTC 4 cut(s) 39, 695, 1256, 1638
BstMBI GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
BstMWI GCNNNNNNNGC 2 cut(s) 1426, 1497
BstNI CCWGG 5 cut(s) 640, 792, 1014, 1203, 1256
BstNSI RCATGY 2 cut(s) 1596, 1670
BstSCI CCNGG 8 cut(s) 638, 790, 921, 922, 1012, 1201, 1254, 1710
BstSFI CTRYAG 2 cut(s) 61, 333
BstUI CGCG 4 cut(s) 598, 769, 1194, 1412
BstV1I GCAGC 4 cut(s) 131, 172, 1432, 1701
BstX2I RGATCY 2 cut(s) 787, 1211
BstYI RGATCY 2 cut(s) 787, 1211
BsuI GTATCC 1 cut(s) 1097
BsuRI GGCC 2 cut(s) 829, 1710
BtgI CCRYGG 1 cut(s) 1410
BtgZI GCGATG 1 cut(s) 1209
BtrI CACGTC 1 cut(s) 1131
BtsCI GGATG 2 cut(s) 798, 1329
BtsIMutI CAGTG 7 cut(s) 272, 728, 1037, 1121, 1354, 1484, 1619
Cac8I GCNNGC 7 cut(s) 144, 148, 300, 439, 524, 1502, 1523
CaiI CAGNNNCTG 3 cut(s) 122, 166, 1649
CciI TCATGA 1 cut(s) 1879
CfoI GCGC 3 cut(s) 769, 945, 1493
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 758, 1709
Cfr42I CCGCGG 1 cut(s) 1413
Cfr9I CCCGGG 1 cut(s) 922
CseI GACGC 2 cut(s) 587, 1395
Csp6I GTAC 8 cut(s) 112, 412, 584, 671, 841, 1381, 1543, 1801
CspCI CAANNNNNGTGG 2 cut(s) 525, 560
CviAII CATG 5 cut(s) 964, 1571, 1593, 1667, 1880
CviQI GTAC 8 cut(s) 112, 412, 584, 671, 841, 1381, 1543, 1801
DpnI GATC 6 cut(s) 126, 482, 715, 789, 1122, 1213
DpnII GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
EaeI YGGCCR 1 cut(s) 827
Eam1104I CTCTTC 1 cut(s) 1802
EarI CTCTTC 1 cut(s) 1802
EciI GGCGGA 2 cut(s) 509, 1274
Eco130I CCWWGG 1 cut(s) 1430
Eco31I GGTCTC 2 cut(s) 39, 695
Eco47I GGWCC 1 cut(s) 758
Eco47III AGCGCT 1 cut(s) 944
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 741, 922
EcoRII CCWGG 5 cut(s) 638, 790, 1012, 1201, 1254
EcoT14I CCWWGG 1 cut(s) 1430
ErhI CCWWGG 1 cut(s) 1430
FaeI CATG 5 cut(s) 967, 1574, 1596, 1670, 1883
FaqI GGGAC 1 cut(s) 1219
FatI CATG 5 cut(s) 963, 1570, 1592, 1666, 1879
FauI CCCGC 3 cut(s) 515, 603, 777
FbaI TGATCA 2 cut(s) 124, 480
Fnu4HI GCNGC 7 cut(s) 120, 161, 164, 649, 1240, 1446, 1690
FokI GGATG 2 cut(s) 785, 1336
Fsp4HI GCNGC 7 cut(s) 120, 161, 164, 649, 1240, 1446, 1690
GlaI GCGC 3 cut(s) 768, 944, 1492
GluI GCNGC 7 cut(s) 120, 161, 164, 649, 1240, 1446, 1690
GsuI CTGGAG 2 cut(s) 702, 907
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 2 cut(s) 829, 1710
HapII CCGG 3 cut(s) 147, 923, 1712
HgaI GACGC 2 cut(s) 587, 1395
HhaI GCGC 3 cut(s) 769, 945, 1493
Hin1II CATG 5 cut(s) 967, 1574, 1596, 1670, 1883
Hin6I GCGC 3 cut(s) 767, 943, 1491
HinP1I GCGC 3 cut(s) 767, 943, 1491
HindIII AAGCTT 5 cut(s) 225, 459, 748, 1519, 1529
HinfI GANTC 8 cut(s) 175, 260, 428, 932, 1232, 1609, 1742, 1832
HpaII CCGG 3 cut(s) 147, 923, 1712
HphI GGTGA 1 cut(s) 906
Hpy166II GTNNAC 8 cut(s) 114, 196, 359, 761, 843, 1128, 1552, 1892
Hpy188I TCNGA 8 cut(s) 39, 294, 405, 579, 1082, 1375, 1739, 1747
Hpy188III TCNNGA 5 cut(s) 206, 886, 1187, 1644, 1880
Hpy8I GTNNAC 8 cut(s) 114, 196, 359, 761, 843, 1128, 1552, 1892
Hpy99I CGWCG 3 cut(s) 155, 371, 1789
HpyAV CCTTC 7 cut(s) 248, 607, 1225, 1361, 1506, 1816, 1846
HpyCH4III ACNGT 8 cut(s) 131, 634, 775, 1116, 1349, 1479, 1568, 1651
HpyCH4IV ACGT 1 cut(s) 1130
HpyF10VI GCNNNNNNNGC 2 cut(s) 1426, 1497
HpySE526I ACGT 1 cut(s) 1130
Hsp92II CATG 5 cut(s) 967, 1574, 1596, 1670, 1883
HspAI GCGC 3 cut(s) 767, 943, 1491
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 2 cut(s) 124, 480
KspI CCGCGG 1 cut(s) 1413
Kzo9I GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
LguI GCTCTTC 1 cut(s) 1802
LmnI GCTCC 5 cut(s) 388, 406, 888, 1141, 1728
Lsp1109I GCAGC 4 cut(s) 131, 172, 1432, 1701
LweI GCATC 2 cut(s) 1160, 1186
MaeII ACGT 1 cut(s) 1130
MaeIII GTNAC 4 cut(s) 131, 271, 634, 1131
MalI GATC 6 cut(s) 126, 482, 715, 789, 1122, 1213
MboI GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
MboII GAAGA 3 cut(s) 269, 317, 1819
MflI RGATCY 2 cut(s) 787, 1211
MhlI GDGCHC 1 cut(s) 1356
MlsI TGGCCA 1 cut(s) 829
MluCI AATT 6 cut(s) 41, 343, 556, 708, 863, 1385
MluNI TGGCCA 1 cut(s) 829
MlyI GAGTC 1 cut(s) 926
MmeI TCCRAC 2 cut(s) 1706, 1770
MnlI CCTC 7 cut(s) 49, 737, 750, 922, 1356, 1676, 1679
Mox20I TGGCCA 1 cut(s) 829
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 829
MseI TTAA 3 cut(s) 21, 573, 1776
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 829
MspA1I CMGCKG 3 cut(s) 122, 166, 1412
MspI CCGG 3 cut(s) 147, 923, 1712
MspR9I CCNGG 8 cut(s) 640, 792, 923, 924, 1014, 1203, 1256, 1712
MvaI CCWGG 5 cut(s) 640, 792, 1014, 1203, 1256
MvnI CGCG 4 cut(s) 598, 769, 1194, 1412
MwoI GCNNNNNNNGC 2 cut(s) 1426, 1497
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 3 cut(s) 923, 924, 1712
NdeII GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 5 cut(s) 967, 1574, 1596, 1670, 1883
NlaIV GGNNCC 7 cut(s) 247, 384, 789, 877, 1143, 1213, 1730
NmuCI GTSAC 2 cut(s) 131, 1131
NspI RCATGY 2 cut(s) 1596, 1670
PaeR7I CTCGAG 1 cut(s) 741
PagI TCATGA 1 cut(s) 1879
PciI ACATGT 1 cut(s) 1666
PciSI GCTCTTC 1 cut(s) 1802
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 7 cut(s) 175, 260, 428, 1232, 1609, 1742, 1832
PkrI GCNGC 7 cut(s) 121, 162, 165, 650, 1241, 1447, 1691
PleI GAGTC 1 cut(s) 926
PpsI GAGTC 1 cut(s) 926
PscI ACATGT 1 cut(s) 1666
PsiI TTATAA 1 cut(s) 1857
Psp6I CCWGG 5 cut(s) 638, 790, 1012, 1201, 1254
PspGI CCWGG 5 cut(s) 638, 790, 1012, 1201, 1254
PspN4I GGNNCC 7 cut(s) 247, 384, 789, 877, 1143, 1213, 1730
PspPI GGNCC 2 cut(s) 758, 1709
PspXI VCTCGAGB 1 cut(s) 741
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 3 cut(s) 122, 166, 1649
PsuI RGATCY 2 cut(s) 787, 1211
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 8 cut(s) 113, 413, 585, 672, 842, 1382, 1544, 1802
RsaNI GTAC 8 cut(s) 112, 412, 584, 671, 841, 1381, 1543, 1801
RseI CAYNNNNRTG 1 cut(s) 210
SacII CCGCGG 1 cut(s) 1413
SapI GCTCTTC 1 cut(s) 1802
SaqAI TTAA 3 cut(s) 21, 573, 1776
SatI GCNGC 7 cut(s) 120, 161, 164, 649, 1240, 1446, 1690
Sau3AI GATC 6 cut(s) 124, 480, 713, 787, 1120, 1211
Sau96I GGNCC 2 cut(s) 758, 1709
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 8 cut(s) 640, 792, 923, 924, 1014, 1203, 1256, 1712
SduI GDGCHC 1 cut(s) 1356
SfaNI GCATC 2 cut(s) 1160, 1186
SfcI CTRYAG 2 cut(s) 61, 333
Sfr274I CTCGAG 1 cut(s) 741
Sfr303I CCGCGG 1 cut(s) 1413
SgrAI CRCCGGYG 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 1413
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 741
SmaI CCCGGG 1 cut(s) 924
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 4 cut(s) 606, 692, 741, 1846
SmoI CTYRAG 4 cut(s) 606, 692, 741, 1846
Sse9I AATT 6 cut(s) 41, 343, 556, 708, 863, 1385
StyD4I CCNGG 8 cut(s) 638, 790, 921, 922, 1012, 1201, 1254, 1710
StyI CCWWGG 1 cut(s) 1430
TaaI ACNGT 8 cut(s) 131, 634, 775, 1116, 1349, 1479, 1568, 1651
TaiI ACGT 1 cut(s) 1133
TaqI TCGA 6 cut(s) 350, 530, 681, 712, 742, 1310
TaqII GACCGA 1 cut(s) 845
TasI AATT 6 cut(s) 41, 343, 556, 708, 863, 1385
TatI WGTACW 6 cut(s) 111, 411, 840, 1380, 1542, 1800
TauI GCSGC 3 cut(s) 166, 651, 1242
TfiI GAWTC 7 cut(s) 175, 260, 428, 1232, 1609, 1742, 1832
Tru1I TTAA 3 cut(s) 21, 573, 1776
Tru9I TTAA 3 cut(s) 21, 573, 1776
TscAI CASTG 7 cut(s) 272, 735, 1044, 1121, 1354, 1484, 1619
TseFI GTSAC 2 cut(s) 131, 1131
TseI GCWGC 4 cut(s) 119, 160, 1445, 1689
Tsp45I GTSAC 2 cut(s) 131, 1131
TspDTI ATGAA 7 cut(s) 336, 492, 639, 787, 1008, 1378, 1868
TspGWI ACGGA 2 cut(s) 64, 208
TspMI CCCGGG 1 cut(s) 922
TspRI CASTG 7 cut(s) 272, 735, 1044, 1121, 1354, 1484, 1619
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 3 cut(s) 41, 708, 1385
XceI RCATGY 2 cut(s) 1596, 1670
XhoI CTCGAG 1 cut(s) 741
XmaI CCCGGG 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.