Prupe.5G151500_v2.0.a1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001351
Physical Location & Seq
Forward (+)
13708441 .. 13712546
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G151500.1

Sequence Viewer

Length: 1869 bp
ATGCAGAGGTTCAGCTTCTTGTCCCTGAAAGCTGCACTGATATTGGTTTTCATAGTGCATTTCACGGTAGCTCATAATGTCAAGAGGCATGGAGGGGCAGGTGATTGGGTGCAGCCACTGTCCAAGATTCAAATCCACAAAACCATCGATGCGACTCACGAAAATGCATCCGTCCGCGCGTACCCTGTCCTCCTTGGCACCACGGGGAAAGAATATGAATGGGTAACTGTGAATGTTGTTAGTCCTAATCCTTCTTCAGATGATTGGTTGGGAGTCTTTTCTCCAGCAAAATTCAACGCATCGACCTGCCCATACACTGAAGACTATTATGAGCCATATCTATGCTCAGCTCCTATAAAGTACAAGTATGCCAATTATTCCAATGCTAATTATAACAAGACAGGCAAAACCATTTTGAAATTCCGATTGATCAACCAACGGGCAGATTTTGCATTTGCTTTGTTCTCCGGTGGCCTGTCAAGCCCAAAATTGGTGGCAATTTCTAATGCTATAGTTTTCGCGAATCCGAAGGCACCTCTTTACCCTCGTCTTGCTCTAGCCAAGAGCTGGGATGAAATGACAGTAACATGGACAAGCGGCTATGACATTAGCGAAGCTGTACCTTTTGTTGAGTGGGGTCTCAAGGGAGAAGCTCAAGTTCGATCTCCAGCAGGAACATTGACATTTCCTCGAGGAAGCATGTGTGCTGAACCAGCACGAACGGTTGGTTGGCGCGATCCTGGTTTCTTCCACACAAGTTTTTTAAAGAATTTGTGGCCTAACTCAAAGTACACTTACAAGCTTGGTCATAGGTTATACAATGGTTCCTATATCTGGAGCAAATCCTATGCTTTTACAGCACCACCATATCCAGGGCAGAACTCGTTACAGCGCATTATTGTGTTTGGTGACATGGGAAAGGCTGAACGTGATGGTTCGAATGAATACGCTGATTATCAGCCAGGCTCACTCAACACCACAGATTCACTGATCAAGGACCTAGACAACTTTGACATAGTTTTCCACATAGGAGATATGCCCTATGCCAATGGATACTTGTCACAGTGGGATCAGTTCACATCGCAGGTGGAGCCTATTGCATCAGCTGTGCCGTACATGGTTGCAAGTGGCAACCACGAACGAGATACACCAAACACCGGCGGCTTCTATGACACTAATGATTCGGGTGGTGAATGTGGAGTGCCAGCTGAGACTATGTTTTTTGTTCCTGCTGATAACAGGGCTAAGTTCTGGTATCAAGCAGATTATGGCATGTTCCACTTCTGTATAGCTGACACTGAGCATGACTGGAGGGAAGGTTCTGAGCAGTACCAATTTATTGAGAAATGCCTAGCAGCAGCTGACAGGAACAAACAGCCTTGGCTGATATTCGCTGGTCATCGTGTTCTTGGCTATTCCTCTAACTCTTGGTATGGCCAGGAGGGTTCATTTGAAGAGCCTATGGGAAGAGATGATATGCAAAAGCTTTGGCAAAGGTACAAGGTGGACATTGCATTTTATGGCCATGTACATAACTATGAAAGAATATGCCCCATCTATCAGAACCAATGTGTAAATTCAGAACTATCCCATTATTCAGGAACTGTGAATGGAACAATTCATGTTGTTGTTGGTGGAGGAGGCAGCCACTTATCAGAGTTTAGTACACTAAAGACAGTTTGGAGCGTTTACCAGGATCTTGACTTTGGCTATACAAAACTCACAGCATTTAACCACTCTTATCTTCTCTTTGAGTACATGAAGAGCAGTGATGGGAAGGTCTACGATTCCTTCACTATCTCAAGGGACTACAGGGATGTGTTGGCCTGTGTTCATGATGGCTGTGAACCGACCACTTTGGCTACATGA

Protein Analysis

623

Amino Acids

70.14

Weight (kDa)

5.95

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
AarI CACCTGC 2 cut(s) 89, 1075
AbsI CCTCGAGG 1 cut(s) 690
Acc36I ACCTGC 3 cut(s) 89, 314, 1075
AccB1I GGYRCC 2 cut(s) 197, 532
AccB7I CCANNNNNTGG 1 cut(s) 567
AccI GTMKAC 1 cut(s) 1782
AccII CGCG 4 cut(s) 177, 179, 521, 735
AciI CCGC 3 cut(s) 175, 597, 1161
AclWI GGATC 3 cut(s) 731, 1077, 1704
AcoI YGGCCR 2 cut(s) 1435, 1522
AcsI RAATTY 4 cut(s) 290, 419, 769, 1576
AcuI CTGAAG 2 cut(s) 240, 339
AfiI CCNNNNNNNGG 5 cut(s) 490, 567, 834, 872, 1157
AgsI TTSAA 4 cut(s) 131, 295, 418, 1454
AjnI CCWGG 5 cut(s) 739, 871, 961, 1437, 1692
AjuI GAANNNNNNNTTGG 2 cut(s) 712, 744
AloI GAACNNNNNNTCC 2 cut(s) 1303, 1335
Alw26I GTCTC 2 cut(s) 644, 1205
AlwI GGATC 3 cut(s) 731, 1077, 1704
AlwNI CAGNNNCTG 3 cut(s) 118, 1361, 1604
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 5 cut(s) 472, 776, 1435, 1522, 1824
ApeKI GCWGC 5 cut(s) 32, 112, 1355, 1358, 1644
ApoI RAATTY 4 cut(s) 290, 419, 769, 1576
AspLEI GCGC 3 cut(s) 179, 735, 894
AspS9I GGNCC 1 cut(s) 997
AsuHPI GGTGA 3 cut(s) 113, 920, 1202
AsuII TTCGAA 1 cut(s) 938
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 1 cut(s) 997
BalI TGGCCA 2 cut(s) 1437, 1524
BanI GGYRCC 2 cut(s) 197, 532
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 5 cut(s) 19, 124, 1367, 1370, 1656
BccI CCATC 5 cut(s) 152, 926, 1562, 1766, 1832
BceAI ACGGC 1 cut(s) 1096
BciT130I CCWGG 5 cut(s) 741, 873, 963, 1439, 1694
BciVI GTATCC 1 cut(s) 1046
BclI TGATCA 2 cut(s) 429, 990
BcoDI GTCTC 2 cut(s) 644, 1205
BfaI CTAG 3 cut(s) 557, 1001, 1352
BfmI CTRYAG 2 cut(s) 510, 1810
BfuAI ACCTGC 3 cut(s) 89, 314, 1075
BfuI GTATCC 1 cut(s) 1046
BisI GCNGC 7 cut(s) 33, 113, 598, 1162, 1356, 1359, 1645
BlpI GCTNAGC 1 cut(s) 346
BlsI GCNGC 7 cut(s) 34, 114, 599, 1163, 1357, 1360, 1646
Bme1390I CCNGG 5 cut(s) 741, 873, 963, 1439, 1694
Bme18I GGWCC 1 cut(s) 997
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 997
BmiI GGNNCC 4 cut(s) 199, 534, 826, 1092
BmrFI CCNGG 5 cut(s) 741, 873, 963, 1439, 1694
BmsI GCATC 4 cut(s) 139, 176, 308, 1109
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 4 cut(s) 267, 651, 856, 1330
Bpu1102I GCTNAGC 1 cut(s) 346
Bpu14I TTCGAA 1 cut(s) 938
BpuEI CTTGAG 3 cut(s) 626, 639, 1786
Bsa29I ATCGAT 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 644
BsaJI CCNNGG 4 cut(s) 193, 201, 872, 1379
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 1303, 1333
Bsc4I CCNNNNNNNGG 5 cut(s) 490, 567, 834, 872, 1157
Bse118I RCCGGY 1 cut(s) 1157
Bse1I ACTGG 1 cut(s) 1313
Bse3DI GCAATG 1 cut(s) 1509
Bse8I GATNNNNATC 1 cut(s) 131
BseBI CCWGG 5 cut(s) 741, 873, 963, 1439, 1694
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 4 cut(s) 193, 201, 872, 1379
BseGI GGATG 3 cut(s) 167, 577, 1822
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 5 cut(s) 490, 567, 834, 872, 1157
BseMI GCAATG 1 cut(s) 1509
BseMII CTCAG 4 cut(s) 360, 1200, 1290, 1314
BseNI ACTGG 1 cut(s) 1313
BseRI GAGGAG 1 cut(s) 1653
BseXI GCAGC 5 cut(s) 19, 124, 1367, 1370, 1656
BseYI CCCAGC 1 cut(s) 567
BsgI GTGCAG 2 cut(s) 18, 131
Bsh1236I CGCG 4 cut(s) 177, 179, 521, 735
BshFI GGCC 5 cut(s) 474, 778, 1437, 1524, 1826
BshNI GGYRCC 2 cut(s) 197, 532
BshVI ATCGAT 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 2 cut(s) 468, 1158
BslFI GGGAC 2 cut(s) 7, 1820
BslI CCNNNNNNNGG 5 cut(s) 490, 567, 834, 872, 1157
BsmAI GTCTC 2 cut(s) 644, 1205
BsmFI GGGAC 2 cut(s) 7, 1820
BsnI GGCC 5 cut(s) 474, 778, 1437, 1524, 1826
Bso31I GGTCTC 1 cut(s) 644
BsoBI CYCGRG 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 938
Bsp1407I TGTACA 1 cut(s) 1528
Bsp143I GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
Bsp1720I GCTNAGC 1 cut(s) 346
Bsp68I TCGCGA 1 cut(s) 521
BspACI CCGC 3 cut(s) 175, 597, 1161
BspANI GGCC 5 cut(s) 474, 778, 1437, 1524, 1826
BspCNI CTCAG 4 cut(s) 359, 1201, 1291, 1315
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 4 cut(s) 177, 179, 521, 735
BspHI TCATGA 1 cut(s) 1834
BspLI GGNNCC 4 cut(s) 199, 534, 826, 1092
BspMI ACCTGC 3 cut(s) 89, 314, 1075
BspPI GGATC 3 cut(s) 731, 1077, 1704
BspQI GCTCTTC 2 cut(s) 1449, 1757
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 2 cut(s) 197, 532
BspTNI GGTCTC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 1509
BsrFI RCCGGY 1 cut(s) 1157
BsrGI TGTACA 1 cut(s) 1528
BsrI ACTGG 1 cut(s) 1313
BssAI RCCGGY 1 cut(s) 1157
BssECI CCNNGG 4 cut(s) 193, 201, 872, 1379
BssMI GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
BssT1I CCWWGG 2 cut(s) 193, 1379
Bst2UI CCWGG 5 cut(s) 741, 873, 963, 1439, 1694
Bst4CI ACNGT 8 cut(s) 67, 120, 229, 583, 724, 1065, 1606, 1678
Bst6I CTCTTC 3 cut(s) 1449, 1462, 1757
BstAPI GCANNNNNTGC 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 1528
BstBI TTCGAA 1 cut(s) 938
BstC8I GCNNGC 1 cut(s) 1206
BstDEI CTNAG 5 cut(s) 346, 1209, 1245, 1299, 1323
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 167, 577, 1822
BstFNI CGCG 4 cut(s) 177, 179, 521, 735
BstHHI GCGC 3 cut(s) 179, 735, 894
BstKTI GATC 6 cut(s) 432, 665, 739, 993, 1072, 1699
BstMAI GTCTC 2 cut(s) 644, 1205
BstMBI GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
BstMWI GCNNNNNNNGC 5 cut(s) 449, 480, 713, 857, 1090
BstNI CCWGG 5 cut(s) 741, 873, 963, 1439, 1694
BstNSI RCATGY 2 cut(s) 703, 1276
BstSCI CCNGG 5 cut(s) 739, 871, 961, 1437, 1692
BstSFI CTRYAG 2 cut(s) 510, 1810
BstUI CGCG 4 cut(s) 177, 179, 521, 735
BstV1I GCAGC 5 cut(s) 19, 124, 1367, 1370, 1656
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 1696
BstYI RGATCY 1 cut(s) 1696
Bsu15I ATCGAT 1 cut(s) 147
BsuI GTATCC 1 cut(s) 1046
BsuRI GGCC 5 cut(s) 474, 778, 1437, 1524, 1826
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 1065
BtsCI GGATG 3 cut(s) 167, 577, 1822
BtsI GCAGTG 1 cut(s) 1774
BtsIMutI CAGTG 7 cut(s) 35, 116, 315, 986, 1070, 1296, 1774
BtuMI TCGCGA 1 cut(s) 521
BveI ACCTGC 3 cut(s) 89, 314, 1075
Cac8I GCNNGC 1 cut(s) 1206
CaiI CAGNNNCTG 3 cut(s) 118, 1361, 1604
CciI TCATGA 1 cut(s) 1834
CfoI GCGC 3 cut(s) 179, 735, 894
Cfr10I RCCGGY 1 cut(s) 1157
Cfr13I GGNCC 1 cut(s) 997
ClaI ATCGAT 1 cut(s) 147
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
DdeI CTNAG 5 cut(s) 346, 1209, 1245, 1299, 1323
DpnI GATC 6 cut(s) 431, 664, 738, 992, 1071, 1698
DpnII GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
DraI TTTAAA 1 cut(s) 765
EaeI YGGCCR 2 cut(s) 1435, 1522
Eam1104I CTCTTC 3 cut(s) 1449, 1462, 1757
EarI CTCTTC 3 cut(s) 1449, 1462, 1757
Eco130I CCWWGG 2 cut(s) 193, 1379
Eco31I GGTCTC 1 cut(s) 644
Eco47I GGWCC 1 cut(s) 997
Eco57I CTGAAG 2 cut(s) 240, 339
Eco88I CYCGRG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 997
EcoRII CCWGG 5 cut(s) 739, 871, 961, 1437, 1692
EcoT14I CCWWGG 2 cut(s) 193, 1379
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 193, 1379
FaqI GGGAC 2 cut(s) 7, 1820
FbaI TGATCA 2 cut(s) 429, 990
FblI GTMKAC 1 cut(s) 1782
Fnu4HI GCNGC 7 cut(s) 33, 113, 598, 1162, 1356, 1359, 1645
FokI GGATG 3 cut(s) 154, 584, 1829
Fsp4HI GCNGC 7 cut(s) 33, 113, 598, 1162, 1356, 1359, 1645
FspBI CTAG 3 cut(s) 557, 1001, 1352
GlaI GCGC 3 cut(s) 178, 734, 893
GluI GCNGC 7 cut(s) 33, 113, 598, 1162, 1356, 1359, 1645
GsaI CCCAGC 1 cut(s) 571
GsuI CTGGAG 4 cut(s) 267, 651, 856, 1330
HaeIII GGCC 5 cut(s) 474, 778, 1437, 1524, 1826
HapII CCGG 2 cut(s) 468, 1158
HhaI GCGC 3 cut(s) 179, 735, 894
Hin6I GCGC 3 cut(s) 177, 733, 892
HinP1I GCGC 3 cut(s) 177, 733, 892
HindIII AAGCTT 2 cut(s) 800, 1484
HinfI GANTC 7 cut(s) 127, 154, 273, 523, 983, 1181, 1787
HpaII CCGG 2 cut(s) 468, 1158
HphI GGTGA 3 cut(s) 113, 920, 1202
Hpy166II GTNNAC 7 cut(s) 792, 1077, 1507, 1667, 1690, 1783, 1847
Hpy188I TCNGA 7 cut(s) 259, 425, 528, 1324, 1563, 1582, 1657
Hpy188III TCNNGA 7 cut(s) 82, 158, 520, 835, 1599, 1700, 1835
Hpy8I GTNNAC 7 cut(s) 792, 1077, 1507, 1667, 1690, 1783, 1847
HpyAV CCTTC 5 cut(s) 261, 523, 1310, 1771, 1801
HpyCH4III ACNGT 8 cut(s) 67, 120, 229, 583, 724, 1065, 1606, 1678
HpyCH4IV ACGT 1 cut(s) 928
HpyF10VI GCNNNNNNNGC 5 cut(s) 449, 480, 713, 857, 1090
HpyF3I CTNAG 5 cut(s) 346, 1209, 1245, 1299, 1323
HpySE526I ACGT 1 cut(s) 928
HspAI GCGC 3 cut(s) 177, 733, 892
Ksp22I TGATCA 2 cut(s) 429, 990
Kzo9I GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
LguI GCTCTTC 2 cut(s) 1449, 1757
LmnI GCTCC 4 cut(s) 355, 837, 1090, 1683
Lsp1109I GCAGC 5 cut(s) 19, 124, 1367, 1370, 1656
LweI GCATC 4 cut(s) 139, 176, 308, 1109
MaeI CTAG 3 cut(s) 557, 1001, 1352
MaeII ACGT 1 cut(s) 928
MaeIII GTNAC 5 cut(s) 223, 583, 885, 908, 1059
MalI GATC 6 cut(s) 431, 664, 738, 992, 1071, 1698
MboI GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
MboII GAAGA 7 cut(s) 246, 332, 739, 1466, 1479, 1736, 1774
MflI RGATCY 1 cut(s) 1696
MlsI TGGCCA 2 cut(s) 1437, 1524
MluNI TGGCCA 2 cut(s) 1437, 1524
MlyI GAGTC 2 cut(s) 148, 282
Mox20I TGGCCA 2 cut(s) 1437, 1524
Mph1103I ATGCAT 1 cut(s) 169
MscI TGGCCA 2 cut(s) 1437, 1524
MseI TTAA 2 cut(s) 764, 1731
MslI CAYNNNNRTG 4 cut(s) 162, 340, 899, 1536
Msp20I TGGCCA 2 cut(s) 1437, 1524
MspA1I CMGCKG 3 cut(s) 1106, 1208, 1361
MspI CCGG 2 cut(s) 468, 1158
MspR9I CCNGG 5 cut(s) 741, 873, 963, 1439, 1694
MvaI CCWGG 5 cut(s) 741, 873, 963, 1439, 1694
MvnI CGCG 4 cut(s) 177, 179, 521, 735
MwoI GCNNNNNNNGC 5 cut(s) 449, 480, 713, 857, 1090
NdeII GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
NlaIV GGNNCC 4 cut(s) 199, 534, 826, 1092
NmuCI GTSAC 2 cut(s) 908, 1059
NruI TCGCGA 1 cut(s) 521
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 703, 1276
NspV TTCGAA 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 690
PagI TCATGA 1 cut(s) 1834
PaqCI CACCTGC 2 cut(s) 89, 1075
PciSI GCTCTTC 2 cut(s) 1449, 1757
PfeI GAWTC 5 cut(s) 127, 523, 983, 1181, 1787
PflMI CCANNNNNTGG 1 cut(s) 567
PkrI GCNGC 7 cut(s) 34, 114, 599, 1163, 1357, 1360, 1646
PleI GAGTC 2 cut(s) 148, 281
PpsI GAGTC 2 cut(s) 148, 281
PpuMI RGGWCCY 1 cut(s) 997
PsiI TTATAA 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 5 cut(s) 739, 871, 961, 1437, 1692
PspFI CCCAGC 1 cut(s) 567
PspGI CCWGG 5 cut(s) 739, 871, 961, 1437, 1692
PspN4I GGNNCC 4 cut(s) 199, 534, 826, 1092
PspPI GGNCC 1 cut(s) 997
PspPPI RGGWCCY 1 cut(s) 997
PspXI VCTCGAGB 1 cut(s) 690
PstNI CAGNNNCTG 3 cut(s) 118, 1361, 1604
PsuI RGATCY 1 cut(s) 1696
PvuII CAGCTG 3 cut(s) 1106, 1208, 1361
RruI TCGCGA 1 cut(s) 521
RseI CAYNNNNRTG 4 cut(s) 162, 340, 899, 1536
SapI GCTCTTC 2 cut(s) 1449, 1757
SaqAI TTAA 2 cut(s) 764, 1731
SatI GCNGC 7 cut(s) 33, 113, 598, 1162, 1356, 1359, 1645
Sau3AI GATC 6 cut(s) 429, 662, 736, 990, 1069, 1696
Sau96I GGNCC 1 cut(s) 997
SchI GAGTC 2 cut(s) 148, 282
ScrFI CCNGG 5 cut(s) 741, 873, 963, 1439, 1694
SfaNI GCATC 4 cut(s) 139, 176, 308, 1109
SfcI CTRYAG 2 cut(s) 510, 1810
Sfr274I CTCGAG 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 938
SgrAI CRCCGGYG 1 cut(s) 1157
SinI GGWCC 1 cut(s) 997
SlaI CTCGAG 1 cut(s) 690
SmiMI CAYNNNNRTG 4 cut(s) 162, 340, 899, 1536
SmlI CTYRAG 4 cut(s) 641, 654, 690, 1801
SmoI CTYRAG 4 cut(s) 641, 654, 690, 1801
SsiI CCGC 3 cut(s) 175, 597, 1161
SspMI CTAG 3 cut(s) 557, 1001, 1352
StyD4I CCNGG 5 cut(s) 739, 871, 961, 1437, 1692
StyI CCWWGG 2 cut(s) 193, 1379
TaaI ACNGT 8 cut(s) 67, 120, 229, 583, 724, 1065, 1606, 1678
TaiI ACGT 1 cut(s) 931
TaqI TCGA 5 cut(s) 147, 302, 661, 691, 938
TatI WGTACW 5 cut(s) 360, 789, 1528, 1664, 1755
TauI GCSGC 2 cut(s) 600, 1164
TfiI GAWTC 5 cut(s) 127, 523, 983, 1181, 1787
Tru1I TTAA 2 cut(s) 764, 1731
Tru9I TTAA 2 cut(s) 764, 1731
TscAI CASTG 7 cut(s) 42, 123, 322, 993, 1070, 1303, 1774
TseFI GTSAC 2 cut(s) 908, 1059
TseI GCWGC 5 cut(s) 32, 112, 1355, 1358, 1644
Tsp45I GTSAC 2 cut(s) 908, 1059
TspDTI ATGAA 9 cut(s) 40, 231, 588, 957, 1437, 1554, 1610, 1775, 1823
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 7 cut(s) 42, 123, 322, 993, 1070, 1303, 1774
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 997
XapI RAATTY 4 cut(s) 290, 419, 769, 1576
XceI RCATGY 2 cut(s) 703, 1276
XhoI CTCGAG 1 cut(s) 690
XmiI GTMKAC 1 cut(s) 1782
XspI CTAG 3 cut(s) 557, 1001, 1352
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.