Rh7AG108800

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001351
Physical Location & Seq
Forward (+)
8584922 .. 8588111
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG108800.1

Sequence Viewer

Length: 1869 bp
ATGGCCTTCTTCTTCCTCCTCAAAGTTGCAGTGCTACTGGTTTTCTTAGGTCAGTTCACAGTTGCTCATAATATCAAGCCGAAGCTGCATGCAGATGGTGGTGATGGAGTGCAGCCGCTGTCCAAGATTCAAATCCTCAGAGCTGTTTCTCAGCTTCACGAAAATGCCTCCATCTCTGCGTACCCTGTCCTTCTTGGCACAAAAGGGGAAGATCTTGGGTGGGTAACTGTAACAATTTCTAGTCCTAGTCCTGCACCAGATGATTGGGTGGCAGTTTTTTCTCCTGCAGTTTTCAATGCATCGACCTGTCCATACAATGAAGATTACTATGAGCCGTACATTTGCACATCTCCTATAAAGTACAAGTTTGCCAATTATTCCAGTGCTGATTACGCAACCACTGGCAATTCAACTTTGAAGTTCCTGTTGATCAATCAACGGGCAGATTTTTCGTTTGCATTGTTCTCCGGCGGGCTGTCAAACCCGAAATTGGTGGCAGTTTCTAACAATGTAACCTTTGCGTATCCGAAGGCACCCCTTTATCCTCGCCTTGCTCTAGGGAAGCTCTGGGATGAAATGACAGTGACTTGGACAAGCGGCTATAACATCGATGAAGCTGTACCTTTAGTTGAGTGGGGTTTAGAGGGAGAGCCTCAAACTCGATCTCCAGCAGGAACATTGACTTTCAGTCGACGGGACATGTGTGCCGCACCCGCACGAACAGTTGGTTGGCGTGATCCTGGTTTCATCCATACAAGTTTTCTAAAAGATTTGTGGCCAAATTCAGTGTACTCTTACAAGCTTGGTCATAGGTTATCTAATGGTTCCTATATCTGGAGCAAGGCTTACTCTTTCAAGTCATCACCATATCCTGGGCAGGAGTCATTGCAGCGTGTTATTATATTTGGTGACATGGGAAAGGCTGAGCGTGATGGTTCAAATGAATATGCTGATTATCAGCCCGGCGCACTCAACACCACAGATCAGCTAATCAAGGACTTGGACAACATTGACATAGTTTTTCACATAGGAGATATGGCTTATGCCAATGGATACATCTCACAGTGGGATCAGTTCACATCACAGGTGGAGCCGATTTCATCAGTTGTACCATATATGATTGCGAGTGGCAACCATGAACGAGATACAACAGGGACCGGATCCTTCTACGACACTAATGATGCAGGTGGCGAATGTGGTGTGCCAGCTGAGACCATGTATTATGTTCCTGCTGAGAACAGAGCTAAATTCTGGTATGCAACAGATTATGGCATGTTCCACTTCTGTATAGCTGACACTGAGCATGACTGGAGAGAAGGTTCTGAGCAGTACAAATTTATTGAGAAATGCCTTGCATCTGCCAATAGGCACAAGCAGCCTTGGCTGATATTCGCTGCTCATCGTGTTCTCGGTTATTCCTCTAACTCTTGGTATGGACAAGAGGGTTCATTTGATGAGCCCATGGGAAGGGATGACATGCAAAAGCTTTGGCAAAGATACAAGGTGGACATTGCATTTTACGGCCATGTCCATAACTACGAAAGAATATGCCCCATCTATCAGAACCAGTGTGTAACTGCGGAAATCTCTAACTATTCAGGAACTGTCAACGGAACAATCCATGTTGTTGTTGGTGGAGGAGGCAGCCATTTATCAGATTTTAGTTCGCTGCACACAGTTTGGAGCATTTACCAAGATCGAGATTATGGTTATACGAAACTCACAGCATATAACCACTCATCGCTTCTCTTGGAGTACAAGAAGAGCAGCGACGGAAAGGTCTATGATTCATTCACCATCTCAAGGGACTACAGGGATGTCTTGGCCTGTGTTCATGATGGTTGTGAACCAACCACCTTAGCTACATGA

Protein Analysis

622

Amino Acids

69.65

Weight (kDa)

5.44

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 58 - 174 9.2e-44 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 182 - 286 3.9e-12 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 298 - 513 2.1e-16 Calcineurin-like phosphoesterase
Metallophos_C PF14008 538 - 597 2.6e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1175
AasI GACNNNNNNGTC 1 cut(s) 1778
Acc36I ACCTGC 1 cut(s) 1175
AccB1I GGYRCC 1 cut(s) 532
AccB7I CCANNNNNTGG 1 cut(s) 872
AccI GTMKAC 1 cut(s) 691
AciI CCGC 6 cut(s) 116, 471, 597, 708, 714, 1580
AclWI GGATC 4 cut(s) 731, 1077, 1155, 1168
AcoI YGGCCR 2 cut(s) 776, 1522
AcsI RAATTY 3 cut(s) 781, 1247, 1334
AfaI GTAC 8 cut(s) 182, 338, 362, 621, 791, 1110, 1331, 1757
AfiI CCNNNNNNNGG 5 cut(s) 490, 834, 872, 1467, 1803
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 6 cut(s) 131, 295, 411, 418, 856, 939
AhdI GACNNNNNGTC 1 cut(s) 687
AjnI CCWGG 2 cut(s) 739, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 712, 744
AloI GAACNNNNNNTCC 2 cut(s) 1303, 1335
Alw26I GTCTC 1 cut(s) 1205
AlwI GGATC 4 cut(s) 731, 1077, 1155, 1168
AlwNI CAGNNNCTG 2 cut(s) 118, 1604
AoxI GGCC 4 cut(s) 3, 776, 1522, 1824
ApeKI GCWGC 8 cut(s) 85, 112, 889, 1375, 1394, 1644, 1669, 1767
ApoI RAATTY 3 cut(s) 781, 1247, 1334
AspLEI GCGC 1 cut(s) 968
AspS9I GGNCC 1 cut(s) 1155
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 4 cut(s) 113, 855, 920, 1786
AvaII GGWCC 1 cut(s) 1155
BalI TGGCCA 1 cut(s) 778
BamHI GGATCC 1 cut(s) 1160
BanI GGYRCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 1461
BbvI GCAGC 8 cut(s) 72, 124, 901, 1381, 1387, 1656, 1656, 1779
BccI CCATC 7 cut(s) 89, 98, 179, 926, 1562, 1805, 1832
BceAI ACGGC 2 cut(s) 319, 1537
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciT130I CCWGG 2 cut(s) 741, 873
BciVI GTATCC 2 cut(s) 534, 1046
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 1 cut(s) 1205
BfaI CTAG 3 cut(s) 240, 246, 557
BfmI CTRYAG 2 cut(s) 285, 1810
BfuAI ACCTGC 1 cut(s) 1175
BfuI GTATCC 2 cut(s) 534, 1046
BglII AGATCT 1 cut(s) 211
BlpI GCTNAGC 1 cut(s) 924
Bme1390I CCNGG 3 cut(s) 741, 873, 963
Bme18I GGWCC 1 cut(s) 1155
BmeRI GACNNNNNGTC 1 cut(s) 687
BmgT120I GGNCC 1 cut(s) 1155
BmiI GGNNCC 5 cut(s) 534, 826, 1092, 1156, 1162
BmrFI CCNGG 3 cut(s) 741, 873, 963
BmsI GCATC 3 cut(s) 308, 1171, 1364
BpmI CTGGAG 3 cut(s) 651, 856, 1330
Bpu10I CCTNAGC 1 cut(s) 1858
Bpu1102I GCTNAGC 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 1786
BpuMI CCSGG 1 cut(s) 963
Bsa29I ATCGAT 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 3 cut(s) 872, 1379, 1461
BsaWI WCCGGW 1 cut(s) 1157
BsaXI ACNNNNNCTCC 4 cut(s) 649, 679, 1303, 1333
Bsc4I CCNNNNNNNGG 5 cut(s) 490, 834, 872, 1467, 1803
Bse1I ACTGG 5 cut(s) 42, 381, 406, 1313, 1567
Bse3DI GCAATG 2 cut(s) 884, 1509
Bse8I GATNNNNATC 1 cut(s) 131
BseBI CCWGG 2 cut(s) 741, 873
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 3 cut(s) 872, 1379, 1461
BseGI GGATG 4 cut(s) 577, 747, 1477, 1822
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 5 cut(s) 490, 834, 872, 1467, 1803
BseMI GCAATG 2 cut(s) 884, 1509
BseMII CTCAG 7 cut(s) 151, 164, 915, 1200, 1224, 1290, 1314
BseNI ACTGG 5 cut(s) 42, 381, 406, 1313, 1567
BseRI GAGGAG 2 cut(s) 8, 1653
BseXI GCAGC 8 cut(s) 72, 124, 901, 1381, 1387, 1656, 1656, 1779
BsgI GTGCAG 3 cut(s) 131, 237, 1655
BshFI GGCC 4 cut(s) 5, 778, 1524, 1826
BshNI GGYRCC 1 cut(s) 532
BshVI ATCGAT 1 cut(s) 609
BsiSI CCGG 3 cut(s) 468, 963, 1158
BslFI GGGAC 3 cut(s) 710, 1168, 1820
BslI CCNNNNNNNGG 5 cut(s) 490, 834, 872, 1467, 1803
BsmAI GTCTC 1 cut(s) 1205
BsmFI GGGAC 3 cut(s) 710, 1168, 1820
BsnI GGCC 4 cut(s) 5, 778, 1524, 1826
Bso31I GGTCTC 1 cut(s) 1205
Bsp1286I GDGCHC 1 cut(s) 1461
Bsp143I GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
Bsp1720I GCTNAGC 1 cut(s) 924
Bsp19I CCATGG 1 cut(s) 1461
BspACI CCGC 6 cut(s) 116, 471, 597, 708, 714, 1580
BspANI GGCC 4 cut(s) 5, 778, 1524, 1826
BspCNI CTCAG 7 cut(s) 150, 163, 916, 1201, 1225, 1291, 1315
BspDI ATCGAT 1 cut(s) 609
BspHI TCATGA 1 cut(s) 1834
BspLI GGNNCC 5 cut(s) 534, 826, 1092, 1156, 1162
BspMAI CTGCAG 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 1175
BspPI GGATC 4 cut(s) 731, 1077, 1155, 1168
BspQI GCTCTTC 1 cut(s) 1757
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 1205
BsrDI GCAATG 2 cut(s) 884, 1509
BsrI ACTGG 5 cut(s) 42, 381, 406, 1313, 1567
BssECI CCNNGG 3 cut(s) 872, 1379, 1461
BssMI GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
BssT1I CCWWGG 2 cut(s) 1379, 1461
Bst2UI CCWGG 2 cut(s) 741, 873
Bst4CI ACNGT 7 cut(s) 61, 229, 583, 724, 1065, 1606, 1678
Bst6I CTCTTC 1 cut(s) 1757
BstC8I GCNNGC 3 cut(s) 90, 473, 1206
BstDEI CTNAG 9 cut(s) 46, 137, 150, 924, 1209, 1233, 1299, 1323, 1858
BstDSI CCRYGG 1 cut(s) 1461
BstF5I GGATG 4 cut(s) 577, 747, 1477, 1822
BstHHI GCGC 1 cut(s) 968
BstKTI GATC 8 cut(s) 214, 432, 665, 739, 985, 1072, 1163, 1699
BstMAI GTCTC 1 cut(s) 1205
BstMBI GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
BstMWI GCNNNNNNNGC 5 cut(s) 85, 392, 713, 1375, 1381
BstNI CCWGG 2 cut(s) 741, 873
BstNSI RCATGY 4 cut(s) 92, 703, 1276, 1480
BstSCI CCNGG 3 cut(s) 739, 871, 961
BstSFI CTRYAG 2 cut(s) 285, 1810
BstV1I GCAGC 8 cut(s) 72, 124, 901, 1381, 1387, 1656, 1656, 1779
BstX2I RGATCY 2 cut(s) 211, 1160
BstXI CCANNNNNNTGG 1 cut(s) 264
BstYI RGATCY 2 cut(s) 211, 1160
Bsu15I ATCGAT 1 cut(s) 609
BsuI GTATCC 2 cut(s) 534, 1046
BsuRI GGCC 4 cut(s) 5, 778, 1524, 1826
BsuTUI ATCGAT 1 cut(s) 609
BtgI CCRYGG 1 cut(s) 1461
BtgZI GCGATG 1 cut(s) 1725
BtsCI GGATG 4 cut(s) 577, 747, 1477, 1822
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 8 cut(s) 36, 388, 399, 588, 792, 1070, 1296, 1574
BveI ACCTGC 1 cut(s) 1175
Cac8I GCNNGC 3 cut(s) 90, 473, 1206
CaiI CAGNNNCTG 2 cut(s) 118, 1604
CciI TCATGA 1 cut(s) 1834
CfoI GCGC 1 cut(s) 968
Cfr13I GGNCC 1 cut(s) 1155
ClaI ATCGAT 1 cut(s) 609
Csp6I GTAC 8 cut(s) 181, 337, 361, 620, 790, 1109, 1330, 1756
CviQI GTAC 8 cut(s) 181, 337, 361, 620, 790, 1109, 1330, 1756
DdeI CTNAG 9 cut(s) 46, 137, 150, 924, 1209, 1233, 1299, 1323, 1858
DpnI GATC 8 cut(s) 213, 431, 664, 738, 984, 1071, 1162, 1698
DpnII GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
DrdI GACNNNNNNGTC 1 cut(s) 1778
DriI GACNNNNNGTC 1 cut(s) 687
DseDI GACNNNNNNGTC 1 cut(s) 1778
EaeI YGGCCR 2 cut(s) 776, 1522
Eam1104I CTCTTC 1 cut(s) 1757
Eam1105I GACNNNNNGTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 1757
Eco130I CCWWGG 2 cut(s) 1379, 1461
Eco24I GRGCYC 1 cut(s) 1461
Eco31I GGTCTC 1 cut(s) 1205
Eco47I GGWCC 1 cut(s) 1155
EcoRII CCWGG 2 cut(s) 739, 871
EcoT14I CCWWGG 2 cut(s) 1379, 1461
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 1461
ErhI CCWWGG 2 cut(s) 1379, 1461
FaqI GGGAC 3 cut(s) 710, 1168, 1820
FauI CCCGC 2 cut(s) 464, 721
FbaI TGATCA 1 cut(s) 429
FblI GTMKAC 1 cut(s) 691
FokI GGATG 4 cut(s) 584, 734, 1484, 1829
FriOI GRGCYC 1 cut(s) 1461
FspBI CTAG 3 cut(s) 240, 246, 557
GlaI GCGC 1 cut(s) 967
GsuI CTGGAG 3 cut(s) 651, 856, 1330
HaeIII GGCC 4 cut(s) 5, 778, 1524, 1826
HapII CCGG 3 cut(s) 468, 963, 1158
HhaI GCGC 1 cut(s) 968
Hin6I GCGC 1 cut(s) 966
HinP1I GCGC 1 cut(s) 966
HincII GTYRAC 2 cut(s) 692, 1609
HindII GTYRAC 2 cut(s) 692, 1609
HindIII AAGCTT 2 cut(s) 800, 1484
HinfI GANTC 3 cut(s) 127, 881, 1787
HpaII CCGG 3 cut(s) 468, 963, 1158
HphI GGTGA 4 cut(s) 113, 855, 920, 1786
Hpy166II GTNNAC 7 cut(s) 57, 692, 790, 1077, 1507, 1609, 1847
Hpy188I TCNGA 5 cut(s) 140, 528, 1324, 1563, 1657
Hpy188III TCNNGA 5 cut(s) 158, 835, 1599, 1700, 1835
Hpy8I GTNNAC 7 cut(s) 57, 692, 790, 1077, 1507, 1609, 1847
Hpy99I CGWCG 2 cut(s) 696, 1775
HpyAV CCTTC 6 cut(s) 16, 200, 523, 1174, 1310, 1461
HpyCH4III ACNGT 7 cut(s) 61, 229, 583, 724, 1065, 1606, 1678
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 392, 713, 1375, 1381
HpyF3I CTNAG 9 cut(s) 46, 137, 150, 924, 1209, 1233, 1299, 1323, 1858
HspAI GCGC 1 cut(s) 966
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
LguI GCTCTTC 1 cut(s) 1757
LmnI GCTCC 3 cut(s) 837, 1090, 1683
Lsp1109I GCAGC 8 cut(s) 72, 124, 901, 1381, 1387, 1656, 1656, 1779
LweI GCATC 3 cut(s) 308, 1171, 1364
MaeI CTAG 3 cut(s) 240, 246, 557
MaeIII GTNAC 6 cut(s) 223, 229, 511, 583, 908, 1573
MalI GATC 8 cut(s) 213, 431, 664, 738, 984, 1071, 1162, 1698
MboI GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
MboII GAAGA 4 cut(s) 4, 221, 332, 1774
MflI RGATCY 2 cut(s) 211, 1160
MhlI GDGCHC 1 cut(s) 1461
MlsI TGGCCA 1 cut(s) 778
MluCI AATT 7 cut(s) 234, 373, 406, 488, 781, 1247, 1334
MluNI TGGCCA 1 cut(s) 778
MlyI GAGTC 1 cut(s) 890
Mox20I TGGCCA 1 cut(s) 778
Mph1103I ATGCAT 1 cut(s) 301
MscI TGGCCA 1 cut(s) 778
MslI CAYNNNNRTG 2 cut(s) 93, 162
Msp20I TGGCCA 1 cut(s) 778
MspA1I CMGCKG 2 cut(s) 118, 1208
MspI CCGG 3 cut(s) 468, 963, 1158
MspR9I CCNGG 3 cut(s) 741, 873, 963
MvaI CCWGG 2 cut(s) 741, 873
MwoI GCNNNNNNNGC 5 cut(s) 85, 392, 713, 1375, 1381
NciI CCSGG 1 cut(s) 963
NcoI CCATGG 1 cut(s) 1461
NdeII GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
NlaIV GGNNCC 5 cut(s) 534, 826, 1092, 1156, 1162
NmuCI GTSAC 2 cut(s) 583, 908
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 4 cut(s) 92, 703, 1276, 1480
PaeI GCATGC 1 cut(s) 92
PagI TCATGA 1 cut(s) 1834
PaqCI CACCTGC 1 cut(s) 1175
PciI ACATGT 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 1757
PfeI GAWTC 2 cut(s) 127, 1787
PflMI CCANNNNNTGG 1 cut(s) 872
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
PscI ACATGT 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 739, 871
PspGI CCWGG 2 cut(s) 739, 871
PspN4I GGNNCC 5 cut(s) 534, 826, 1092, 1156, 1162
PspPI GGNCC 1 cut(s) 1155
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 2 cut(s) 118, 1604
PsuI RGATCY 2 cut(s) 211, 1160
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 8 cut(s) 182, 338, 362, 621, 791, 1110, 1331, 1757
RsaNI GTAC 8 cut(s) 181, 337, 361, 620, 790, 1109, 1330, 1756
RseI CAYNNNNRTG 2 cut(s) 93, 162
SalI GTCGAC 1 cut(s) 690
SapI GCTCTTC 1 cut(s) 1757
Sau3AI GATC 8 cut(s) 211, 429, 662, 736, 982, 1069, 1160, 1696
Sau96I GGNCC 1 cut(s) 1155
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 3 cut(s) 741, 873, 963
SduI GDGCHC 1 cut(s) 1461
SfaNI GCATC 3 cut(s) 308, 1171, 1364
SfcI CTRYAG 2 cut(s) 285, 1810
SinI GGWCC 1 cut(s) 1155
SmiMI CAYNNNNRTG 2 cut(s) 93, 162
SmlI CTYRAG 1 cut(s) 1801
SmoI CTYRAG 1 cut(s) 1801
SphI GCATGC 1 cut(s) 92
Sse9I AATT 7 cut(s) 234, 373, 406, 488, 781, 1247, 1334
SsiI CCGC 6 cut(s) 116, 471, 597, 708, 714, 1580
SspMI CTAG 3 cut(s) 240, 246, 557
StyD4I CCNGG 3 cut(s) 739, 871, 961
StyI CCWWGG 2 cut(s) 1379, 1461
TaaI ACNGT 7 cut(s) 61, 229, 583, 724, 1065, 1606, 1678
TaqI TCGA 5 cut(s) 302, 609, 661, 691, 1699
TasI AATT 7 cut(s) 234, 373, 406, 488, 781, 1247, 1334
TatI WGTACW 4 cut(s) 360, 789, 1329, 1755
TauI GCSGC 3 cut(s) 118, 600, 710
TfiI GAWTC 2 cut(s) 127, 1787
TscAI CASTG 8 cut(s) 36, 388, 406, 588, 792, 1070, 1303, 1574
TseFI GTSAC 2 cut(s) 583, 908
TseI GCWGC 8 cut(s) 85, 112, 889, 1375, 1394, 1644, 1669, 1767
Tsp45I GTSAC 2 cut(s) 583, 908
TspGWI ACGGA 2 cut(s) 1626, 1788
TspRI CASTG 8 cut(s) 36, 388, 406, 588, 792, 1070, 1303, 1574
Van91I CCANNNNNTGG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 1155
XapI RAATTY 3 cut(s) 781, 1247, 1334
XceI RCATGY 4 cut(s) 92, 703, 1276, 1480
XcmI CCANNNNNNNNNTGG 1 cut(s) 1628
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 3 cut(s) 240, 246, 557
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.