RLG00000004550

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001351
Physical Location & Seq
Reverse (-)
60214972 .. 60217366
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004550

Sequence Viewer

Length: 1197 bp
ATGGAGAGCTTCAGCTTCTTCTTGTCCCTTAAACTTGCCCTGTTTTCGGTTTTCTTATTGAATTTAACAGCAGTTCATAGTCACATTAAGCATGGAGGTGATGGGGTACAGCCACTGTCCAAGATTCAAATCCACAGAGCATTTTACGAGCTTCATGAAAATGCAACAGTCCTTCCTGGCACCAAGGGGGAAGATTCTCAATGGGTAACAGTGGAAGTTGGCACTCCTAAGCTTTCTGAAGATGATTGGTTGGCAGTCTTTTCTCCTGCAAATTTCAACTCATCGACTTGTACACTTAATGATGACCTGTATGCTCCATGTATAGGCACAGCTCCTATAAAGTACAGGTTTGCCAATGATTCCAATTCTGATTATGCAAAGACAGGAAAAACATCTTTAAAATTTCAATTGATCAATCAAAGGGAAGATTTTTCATTTGCATTATTCTCTGGTGGGCTGTCAAATCCAAAATTGGTAGCTGTTTCTAATGTTATAACTTTTGCAAATCCGAAGGCACCCCTTTACCCTCTAGGGAAGCATTGGGATGAAATGACGGTGACCTGGACAAGCGGCTATAATATTGATGAAGCTGTACCTTTTGTTGAATGGGGTATCAAGGGAGAGTCCCAAACTAGCTCTCCAGTAGGAACACTAAATTTTACTCGAGAGACCATGTGCGGTCCACCAGCCCGAACAGTTGGTTGGCGCGATCCTGGTTTCATCCACACAGGTTTTCTGAAAGATTTGTGGCCAAATTCTTTCTTGGCCATAGGTTATCTAGCTAATAGTTCTTATATCTGGAGCAAGTCCTACTCTTTCAAGTCATCACCTTATCCCGGGCAGGACTCATTGCCGCATGTTGTTATATTTGGTGACATGGGAAAGGCCGAGCGTGACGGCTCAAACGAATATGATGATTATCAGCCCGGATCTCTCATTACCAGGGATCAAAGGGTCAAGGAGTTGGATGGAATTGACATAGTTTTCCACATAGGAGATATGGCCTATTCCAATGGATACATCTCGCAGTGGGATCAGTTCACATCGCAGGTGGAGCCGATTGCATCAACTGTACCATATATGGTTGCAAGTAGCAATCATGAAAGAGTTGTACCAGGGACAGGATCCTTCTACGACGGTAATGATTCGGGTTGTGAATGTGGTGTGCTAGCCGAGACGCACATAGAACAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

399

Amino Acids

43.98

Weight (kDa)

4.99

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 58 - 169 1.4e-41 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 180 - 274 2.5e-06 Purple acid Phosphatase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AarI CACCTGC 1 cut(s) 1039
Acc36I ACCTGC 1 cut(s) 1039
AccB1I GGYRCC 2 cut(s) 179, 514
AccII CGCG 1 cut(s) 708
AciI CCGC 3 cut(s) 570, 678, 854
AclWI GGATC 6 cut(s) 704, 937, 954, 1041, 1119, 1132
AcoI YGGCCR 2 cut(s) 749, 765
AcsI RAATTY 5 cut(s) 61, 271, 401, 655, 754
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 6 cut(s) 108, 292, 344, 594, 1074, 1113
AfiI CCNNNNNNNGG 4 cut(s) 46, 323, 836, 1121
AgsI TTSAA 6 cut(s) 61, 128, 277, 407, 605, 820
AjnI CCWGG 5 cut(s) 175, 560, 712, 941, 1114
AjuI GAANNNNNNNTTGG 2 cut(s) 685, 717
Alw26I GTCTC 2 cut(s) 662, 1169
AlwI GGATC 6 cut(s) 704, 937, 954, 1041, 1119, 1132
AlwNI CAGNNNCTG 1 cut(s) 115
Ama87I CYCGRG 2 cut(s) 663, 836
AoxI GGCC 4 cut(s) 749, 765, 885, 1002
ApoI RAATTY 5 cut(s) 61, 271, 401, 655, 754
AspLEI GCGC 1 cut(s) 708
AspS9I GGNCC 1 cut(s) 680
AsuC2I CCSGG 3 cut(s) 837, 838, 927
AsuHPI GGTGA 4 cut(s) 110, 568, 819, 884
AsuNHI GCTAGC 1 cut(s) 1168
AvaI CYCGRG 2 cut(s) 663, 836
AvaII GGWCC 1 cut(s) 680
BalI TGGCCA 2 cut(s) 751, 767
BamHI GGATCC 1 cut(s) 1124
BanI GGYRCC 2 cut(s) 179, 514
BccI CCATC 2 cut(s) 95, 962
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 5 cut(s) 177, 562, 714, 943, 1116
BciVI GTATCC 1 cut(s) 1010
BclI TGATCA 1 cut(s) 411
BcnI CCSGG 3 cut(s) 837, 838, 927
BcoDI GTCTC 2 cut(s) 662, 1169
BfaI CTAG 4 cut(s) 530, 633, 779, 1169
BfuAI ACCTGC 1 cut(s) 1039
BfuI GTATCC 1 cut(s) 1010
BisI GCNGC 2 cut(s) 571, 854
BlsI GCNGC 2 cut(s) 572, 855
Bme1390I CCNGG 8 cut(s) 177, 562, 714, 837, 838, 927, 943, 1116
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 2 cut(s) 663, 836
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 4 cut(s) 181, 516, 1056, 1126
BmrFI CCNGG 8 cut(s) 177, 562, 714, 837, 838, 927, 943, 1116
BmsI GCATC 1 cut(s) 1073
BmtI GCTAGC 1 cut(s) 1172
BpmI CTGGAG 2 cut(s) 624, 820
Bpu10I CCTNAGC 1 cut(s) 228
BpuMI CCSGG 3 cut(s) 837, 838, 927
BsaBI GATNNNNATC 2 cut(s) 128, 918
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 183, 836, 942, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 323, 836, 1121
Bse1I ACTGG 1 cut(s) 641
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 2 cut(s) 128, 918
BseBI CCWGG 5 cut(s) 177, 562, 714, 943, 1116
BseDI CCNNGG 4 cut(s) 183, 836, 942, 1115
BseGI GGATG 3 cut(s) 550, 720, 973
BseJI GATNNNNATC 2 cut(s) 128, 918
BseLI CCNNNNNNNGG 4 cut(s) 46, 323, 836, 1121
BseMI GCAATG 1 cut(s) 848
BseNI ACTGG 1 cut(s) 641
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 4 cut(s) 751, 767, 887, 1004
BshNI GGYRCC 2 cut(s) 179, 514
BsiHKCI CYCGRG 2 cut(s) 663, 836
BsiSI CCGG 2 cut(s) 837, 927
BslFI GGGAC 3 cut(s) 10, 610, 1132
BslI CCNNNNNNNGG 4 cut(s) 46, 323, 836, 1121
BsmAI GTCTC 2 cut(s) 662, 1169
BsmBI CGTCTC 1 cut(s) 1169
BsmFI GGGAC 3 cut(s) 10, 610, 1132
BsnI GGCC 4 cut(s) 751, 767, 887, 1004
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 2 cut(s) 663, 836
Bsp1407I TGTACA 1 cut(s) 290
Bsp143I GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
BspACI CCGC 3 cut(s) 570, 678, 854
BspANI GGCC 4 cut(s) 751, 767, 887, 1004
BspFNI CGCG 1 cut(s) 708
BspHI TCATGA 2 cut(s) 154, 1099
BspLI GGNNCC 4 cut(s) 181, 516, 1056, 1126
BspMI ACCTGC 1 cut(s) 1039
BspOI GCTAGC 1 cut(s) 1172
BspPI GGATC 6 cut(s) 704, 937, 954, 1041, 1119, 1132
BspT107I GGYRCC 2 cut(s) 179, 514
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 848
BsrGI TGTACA 1 cut(s) 290
BsrI ACTGG 1 cut(s) 641
BssECI CCNNGG 4 cut(s) 183, 836, 942, 1115
BssMI GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 5 cut(s) 177, 562, 714, 943, 1116
Bst4CI ACNGT 7 cut(s) 117, 169, 211, 556, 697, 1072, 1139
BstAUI TGTACA 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 1170
BstDEI CTNAG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 3 cut(s) 550, 720, 973
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 1 cut(s) 708
BstKTI GATC 6 cut(s) 414, 712, 932, 949, 1036, 1127
BstMAI GTCTC 2 cut(s) 662, 1169
BstMBI GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
BstMWI GCNNNNNNNGC 1 cut(s) 1054
BstNI CCWGG 5 cut(s) 177, 562, 714, 943, 1116
BstNSI RCATGY 1 cut(s) 860
BstPI GGTNACC 1 cut(s) 556
BstSCI CCNGG 8 cut(s) 175, 560, 712, 835, 836, 925, 941, 1114
BstUI CGCG 1 cut(s) 708
BstX2I RGATCY 2 cut(s) 929, 1124
BstYI RGATCY 2 cut(s) 929, 1124
BsuI GTATCC 1 cut(s) 1010
BsuRI GGCC 4 cut(s) 751, 767, 887, 1004
BtgZI GCGATG 1 cut(s) 1029
BtsCI GGATG 3 cut(s) 550, 720, 973
BtsI GCAGTG 1 cut(s) 1034
BtsIMutI CAGTG 3 cut(s) 113, 216, 1034
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 1 cut(s) 1170
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 2 cut(s) 154, 1099
CfoI GCGC 1 cut(s) 708
Cfr13I GGNCC 1 cut(s) 680
Cfr9I CCCGGG 1 cut(s) 836
CseI GACGC 1 cut(s) 1186
Csp6I GTAC 6 cut(s) 107, 291, 343, 593, 1073, 1112
CviAII CATG 7 cut(s) 92, 155, 318, 673, 857, 877, 1100
CviQI GTAC 6 cut(s) 107, 291, 343, 593, 1073, 1112
DdeI CTNAG 1 cut(s) 228
DpnI GATC 6 cut(s) 413, 711, 931, 948, 1035, 1126
DpnII GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
DraI TTTAAA 1 cut(s) 399
EaeI YGGCCR 2 cut(s) 749, 765
Eco130I CCWWGG 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 662
Eco47I GGWCC 1 cut(s) 680
Eco57I CTGAAG 1 cut(s) 258
Eco88I CYCGRG 2 cut(s) 663, 836
Eco91I GGTNACC 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 556
EcoRII CCWGG 5 cut(s) 175, 560, 712, 941, 1114
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 1169
FaeI CATG 7 cut(s) 95, 158, 321, 676, 860, 880, 1103
FaqI GGGAC 3 cut(s) 10, 610, 1132
FatI CATG 7 cut(s) 91, 154, 317, 672, 856, 876, 1099
FbaI TGATCA 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 571, 854
FokI GGATG 3 cut(s) 557, 707, 980
Fsp4HI GCNGC 2 cut(s) 571, 854
FspBI CTAG 4 cut(s) 530, 633, 779, 1169
GlaI GCGC 1 cut(s) 707
GluI GCNGC 2 cut(s) 571, 854
GsuI CTGGAG 2 cut(s) 624, 820
HaeIII GGCC 4 cut(s) 751, 767, 887, 1004
HapII CCGG 2 cut(s) 837, 927
HgaI GACGC 1 cut(s) 1186
HhaI GCGC 1 cut(s) 708
Hin1II CATG 7 cut(s) 95, 158, 321, 676, 860, 880, 1103
Hin6I GCGC 1 cut(s) 706
HinP1I GCGC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 6 cut(s) 124, 194, 359, 623, 845, 1145
HpaII CCGG 2 cut(s) 837, 927
HphI GGTGA 4 cut(s) 110, 568, 819, 884
Hpy166II GTNNAC 3 cut(s) 293, 683, 1041
Hpy188I TCNGA 4 cut(s) 238, 370, 510, 738
Hpy188III TCNNGA 4 cut(s) 155, 665, 799, 1100
Hpy8I GTNNAC 3 cut(s) 293, 683, 1041
Hpy99I CGWCG 1 cut(s) 1139
HpyAV CCTTC 3 cut(s) 182, 505, 1138
HpyCH4III ACNGT 7 cut(s) 117, 169, 211, 556, 697, 1072, 1139
HpyCH4V TGCA 7 cut(s) 164, 269, 377, 440, 503, 1064, 1088
HpyF10VI GCNNNNNNNGC 1 cut(s) 1054
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 7 cut(s) 95, 158, 321, 676, 860, 880, 1103
HspAI GCGC 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 411
Kzo9I GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
LmnI GCTCC 4 cut(s) 319, 337, 801, 1054
LweI GCATC 1 cut(s) 1073
MaeI CTAG 4 cut(s) 530, 633, 779, 1169
MaeIII GTNAC 5 cut(s) 80, 205, 556, 872, 893
MalI GATC 6 cut(s) 413, 711, 931, 948, 1035, 1126
MboI GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
MboII GAAGA 4 cut(s) 10, 203, 251, 437
MfeI CAATTG 1 cut(s) 407
MflI RGATCY 2 cut(s) 929, 1124
MlsI TGGCCA 2 cut(s) 751, 767
MluCI AATT 9 cut(s) 61, 271, 364, 401, 407, 470, 655, 754, 972
MluNI TGGCCA 2 cut(s) 751, 767
MlyI GAGTC 2 cut(s) 632, 839
MmeI TCCRAC 1 cut(s) 945
MnlI CCTC 2 cut(s) 89, 537
Mox20I TGGCCA 2 cut(s) 751, 767
MscI TGGCCA 2 cut(s) 751, 767
MseI TTAA 5 cut(s) 30, 65, 87, 297, 398
MslI CAYNNNNRTG 3 cut(s) 96, 159, 543
Msp20I TGGCCA 2 cut(s) 751, 767
MspI CCGG 2 cut(s) 837, 927
MspR9I CCNGG 8 cut(s) 177, 562, 714, 837, 838, 927, 943, 1116
MunI CAATTG 1 cut(s) 407
MvaI CCWGG 5 cut(s) 177, 562, 714, 943, 1116
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 1 cut(s) 1054
NciI CCSGG 3 cut(s) 837, 838, 927
NdeII GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
NheI GCTAGC 1 cut(s) 1168
NlaIII CATG 7 cut(s) 95, 158, 321, 676, 860, 880, 1103
NlaIV GGNNCC 4 cut(s) 181, 516, 1056, 1126
NmeAIII GCCGAG 1 cut(s) 913
NmuCI GTSAC 4 cut(s) 80, 556, 872, 893
NspI RCATGY 1 cut(s) 860
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 2 cut(s) 154, 1099
PaqCI CACCTGC 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PfeI GAWTC 4 cut(s) 124, 194, 359, 1145
PkrI GCNGC 2 cut(s) 572, 855
PleI GAGTC 2 cut(s) 631, 839
PpsI GAGTC 2 cut(s) 631, 839
PsiI TTATAA 1 cut(s) 494
Psp6I CCWGG 5 cut(s) 175, 560, 712, 941, 1114
PspEI GGTNACC 1 cut(s) 556
PspGI CCWGG 5 cut(s) 175, 560, 712, 941, 1114
PspN4I GGNNCC 4 cut(s) 181, 516, 1056, 1126
PspPI GGNCC 1 cut(s) 680
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 2 cut(s) 929, 1124
RsaI GTAC 6 cut(s) 108, 292, 344, 594, 1074, 1113
RsaNI GTAC 6 cut(s) 107, 291, 343, 593, 1073, 1112
RseI CAYNNNNRTG 3 cut(s) 96, 159, 543
SaqAI TTAA 5 cut(s) 30, 65, 87, 297, 398
SatI GCNGC 2 cut(s) 571, 854
Sau3AI GATC 6 cut(s) 411, 709, 929, 946, 1033, 1124
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 2 cut(s) 632, 839
ScrFI CCNGG 8 cut(s) 177, 562, 714, 837, 838, 927, 943, 1116
SfaNI GCATC 1 cut(s) 1073
Sfr274I CTCGAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 680
SlaI CTCGAG 1 cut(s) 663
SmaI CCCGGG 1 cut(s) 838
SmiMI CAYNNNNRTG 3 cut(s) 96, 159, 543
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 9 cut(s) 61, 271, 364, 401, 407, 470, 655, 754, 972
SsiI CCGC 3 cut(s) 570, 678, 854
SspI AATATT 1 cut(s) 580
SspMI CTAG 4 cut(s) 530, 633, 779, 1169
StyD4I CCNGG 8 cut(s) 175, 560, 712, 835, 836, 925, 941, 1114
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 7 cut(s) 117, 169, 211, 556, 697, 1072, 1139
TaqI TCGA 2 cut(s) 284, 664
TasI AATT 9 cut(s) 61, 271, 364, 401, 407, 470, 655, 754, 972
TatI WGTACW 2 cut(s) 290, 342
TauI GCSGC 2 cut(s) 573, 856
TfiI GAWTC 4 cut(s) 124, 194, 359, 1145
Tru1I TTAA 5 cut(s) 30, 65, 87, 297, 398
Tru9I TTAA 5 cut(s) 30, 65, 87, 297, 398
TscAI CASTG 3 cut(s) 120, 216, 1034
TseFI GTSAC 4 cut(s) 80, 556, 872, 893
Tsp45I GTSAC 4 cut(s) 80, 556, 872, 893
TspDTI ATGAA 8 cut(s) 65, 143, 171, 423, 561, 600, 709, 1116
TspMI CCCGGG 1 cut(s) 836
TspRI CASTG 3 cut(s) 120, 216, 1034
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 5 cut(s) 61, 271, 401, 655, 754
XceI RCATGY 1 cut(s) 860
XhoI CTCGAG 1 cut(s) 663
XmaI CCCGGG 1 cut(s) 836
XspI CTAG 4 cut(s) 530, 633, 779, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.