FvH4_5g08240

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001351
Physical Location & Seq
Reverse (-)
4780273 .. 4786740
6468 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08240.t1

Sequence Viewer

Length: 3600 bp
ATGTGCAGGGGGGAAGATTTTGGGTGGGTAACAGTGACAGTTTCTTGTCCTAATCCTGCACAAGATGATTGGGTTGCAGTTTTTTCTCCTGCAGATTTTAATGGATCCACCTGTCCAAACAATGAAGACGTCAATAAGTACTATGAGCCCTATATTTGCACATCGCCTATTAAGTACAAGTTTGCCAATTATTCCAGTGCTGATTACGCAACCACTAGTAACTCAACTTTGTACTTTCGACTGATCAATCAACGGGCAGACTTTTCGTTTGCATTGTTCTCTGGCGGGCTGTCAAATCCGAGATTAGTGGCAGTTTCGAATAAGGTGACCTTCGCCTACCCAAAGGCTCCCCTGTACCCACGTCTTGCTCTAGGGAACCTTTGGGATGAAATGACAGTGACTTGGACAAGCGGCTATAACATCGATGAAGCTGTACCTTTAGTTGCGTGGGGTTTAAAGGGGGAGCCTCGAATGCACAACTCTCCGGCAGGAACATTGACTTTCAGTAGACGGGACATGTACTCTTACAAGCTTGGTCATCGGTTATCTAATGGGTCCTATATCTGGAGCAAGATTTACTCTTTCAAGTCAGCACCATATCCAGGGCAGGAGTCATTGCATCGAGTTATCATATTTGGTGACATGGGAAAGGCTGAGCGTGATGGTTCAAATGAATATGCTGATTATCAGCCCGGCTCACTTATCACCACAGATCGGCTAATCAAGGATTTGGACAACTTTGACATAGTTTTTCACATAGGAGATATGCCTTATGCCAATGGATACATATCACAATGGGATCAGTTCACATCACAGGTGGAGCCGATTGCATCAGTTGTACCATATATGGTTGCGAGTGGCAACCATGAACGAGATACAACAGGGACTGGATCCTTCTACGACACTAATGATTCAGGTGGTGAATGTGGTGTGCCAGCTGAAACCATGTATTATGTTCCTGCTGAGAACAGAGCTAAATTCTGGTATGCAACAGATTATGGAATGTTCCACTTCTGTATAGCTGACACTGAGCATGACTGGAGAGAAGGTTCCGAGCAGTACAAATTTATTGAGAAATGCTTTGCATCTGCTAACAGACACAAGCAGCCTTGGCTGATATTTGCTGTTCATCGTGTTCTCGGTTATTCCTCTGACACTTGGTATGTAGATGAGGGTTCATTTGAAGAGGCGATGGGAAGGGATGACATGCAAAAGCTTTGGCAAAGATACAATGTGGACATTGCATTTTACGGCCATGTCCATAACTACGAAAGAATATGCCCCATCTATCAGAACCAGTGTGTAAATTCAGAAATATTCAACTATTCAGGAACTGTCAACGGAACAATCCATGTTGTTGTTGGTGGCGGAGGCAGCCAGTTATCAGATTTTAGTACCCTGAAGACAGTTTGGAGCATCTACCAAGATCTAGACTTTGGTTATACGAAACTCACAGCGTATAACCACTCATCGCTGCTCTTCGAGTACATGAAGAGCAGGGGTGGGAAGGTCTATGATTCATTAGCCATCTCCAGGGACTACAAGGATGTCTTGGCCTGTGTTCATGATGATCAGAAAAACAATAAGAAAAGAATAATTGTTTGCTGTGCTTGTCTTACTTTATACCCACGTGGAAAGAAAAAATTACAGACACATGCACATACAGAGACATGGTTCAAAGCTGGTAGAACTCTTGAAAAATCAGGGAGAGAGCTTTGTTCAACTCCATTCATTGTGTATAAGACACCTATGGAGCAAAGGTTTCGACTTTTCACGATTCTGGTGATTTTGGGGTTCACCCATTTGGGTTTCGCTTGTGCTCAAATCAATGGAGACCAGCCACTGGCCAAGATTCACATTCGGAAAACCACTTTTGCAATTCATGACTCTGCCTCCATTAGAGCCTACCCTTCTGTACTAGGCATACAGGGTGAGGATACAGAATGGGTGACTCTGGATCTTATGTACCCTGATCCTTCTGATAATGACTGGGTTGGAGTCTTTTCTCCTGGAAAACTCAATGCATCACAGTGCCCGCCAGTAGATGGTGAAAGAGAGCAGACTCCATATATATGCACTGCCCCGATAAAGTACAAATATGCAAAACAAGACAATCCAGGTTATACAAAGACTGGCATTGCAACTTTGAAGTTTCAGCTTATAAATCAGCGAGCAGATTTCTCCTTTGCATTATTTTCAGGCGGGTTGTCAAATCCCAAATTGGTGGCAGTATCAAATATCTTGACATTTGCCAATTCTAAAGCGCCTGTCTATCCACGTCTTGCTCAAGGGAAGTCTTGGAATGAAATGACAGTAACTTGGACAAGTGGCTACAGCATAGGCGAAGCTGTTCCATTTGTTGAGTGGGGTATGCAGGGAGATACTCAAAAGCGATCGGCAGCAGCAACATTGACATTTGATCAGAGCAGCTTGTGTGGTTCACCTGCAAGGACAGTTGGGTGGCGCGATCCTGGTTTCATACACACAAGTCTCCTGAAGAACTTGTGGCCAAACACAGTGTACACCTATAGAATGGGTCATCGTCTGTCAGATGGTCAGTATATTCGGAGCAAGGTCTACTCATTCAGATCATCTCCATATCCTGGACAGGATTCCTTGCAACGTGTTGTGATATTTGGTGACATGGGAAAGGCAGAGCGTGATGGTTCAAATGAGTACAGTGATTATCAACCTGGTGCTCTGAACACTACAGATCAAGTAATCAGTGACTTAAACAACATTGACATAGTTTTTCATATAGGAGATCTATCATATGCAAATGGTTATATTTCACAATGGGACCAATTCACAGCACAGGTGGAACCCATTGCATCTACTGTTCCATATATGGTTGGCAGTGGTAATCACGAACGTGATTGGCCTGATTCGGGGTCCTATTACAACGGAATGGATTCAGGTGGAGAATGTGGTGTGCTTGCTGAGACCATGTTTTACGTTCCAGCTAAGAACCGAGCTAAATATTGGTACTCTACAGATTATGGATTGTTTCACTTCTGTATAGCTGACACAGAACATGACTGGAGGGAGGGAACGGAGCAGTACAAGTTCATTGAGAATTGCCTTGCATCTGCGGATAGACAAAAGCAACCTTGGCTGATCTTTGCAGGTCATCGCGTCCTTGGTTATACGTCTGACAAATGGTATAGTCAAGGGGGCGCATTTGAAGAGCCTATGGGAAGAGAAAGCTTGCAGAAACTTTGGCAGAAGTACAAGGTAGACATTGCATTGTATGGCCATGTCCATAACTATGAAAGGACATGCCCCATCTATGAGAACCAATGTGTATCTTCAGAAAGATCTCACTACTCAGGGAAGTTCAATGGAACAATCCATGTTGTTGTTGGAGGAGCCGGAAGCCACTTATCAGACTTCAACCAACAGCAACCAGGTTGGAGTTTCTACAGAGATCAGGACTGGGGATTTGTGAAACTGACAGCATTCAATCACACATCTCTTCTCTTCGAATACAAGAAAAGCAGCGACGGGGAGGTCTACGACTCCTTCACCATATCAAGGGACTACACGGATGTATTGGCTTGTGTTCATGACAGTTGCGAACCAACAACAGCAGCATCTTAG

Protein Analysis

1200

Amino Acids

135.62

Weight (kDa)

5.82

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 3 - 112 2.8e-40 Fn3-like domain from Purple Acid Phosphatase
Metallophos PF00149 208 - 423 1.4e-15 Calcineurin-like phosphoesterase
Metallophos_C PF14008 448 - 507 4.2e-14 Iron/zinc purple acid phosphatase-like protein C
fn3_PAP PF17808 633 - 752 4.7e-45 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 759 - 863 2.9e-13 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 875 - 1090 1e-15 Calcineurin-like phosphoesterase
Metallophos_C PF14008 1115 - 1174 8.5e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2162
AarI CACCTGC 1 cut(s) 2452
AasI GACNNNNNNGTC 1 cut(s) 3509
AatII GACGTC 1 cut(s) 132
Acc36I ACCTGC 2 cut(s) 2452, 3116
AccB7I CCANNNNNTGG 3 cut(s) 1843, 2045, 2603
AccI GTMKAC 4 cut(s) 508, 2577, 3237, 3513
AccII CGCG 2 cut(s) 2466, 3135
AciI CCGC 6 cut(s) 285, 411, 1368, 2036, 2202, 3092
AclWI GGATC 8 cut(s) 99, 112, 807, 885, 898, 1965, 1967, 2462
AcoI YGGCCR 4 cut(s) 1252, 1845, 2507, 3253
AcsI RAATTY 3 cut(s) 977, 1064, 1306
AcuI CTGAAG 3 cut(s) 1421, 2516, 3294
AcvI CACGTG 1 cut(s) 1631
AcyI GRCGYC 1 cut(s) 129
AfiI CCNNNNNNNGG 9 cut(s) 564, 602, 714, 1533, 1843, 2045, 2603, 2888, 3534
AflIII ACRYGT 2 cut(s) 516, 2623
AhlI ACTAGT 1 cut(s) 215
AjiI CACGTC 2 cut(s) 362, 2279
AjnI CCWGG 8 cut(s) 601, 1532, 2008, 2116, 2470, 2602, 2692, 3406
AleI CACNNNNGTG 1 cut(s) 2871
AloI GAACNNNNNNTCC 2 cut(s) 1033, 1065
Alw21I GWGCWC 2 cut(s) 1822, 2701
Alw26I GTCTC 4 cut(s) 1661, 1827, 2495, 2936
AlwI GGATC 8 cut(s) 99, 112, 807, 885, 898, 1965, 1967, 2462
AlwNI CAGNNNCTG 3 cut(s) 887, 1334, 1843
AoxI GGCC 6 cut(s) 1252, 1554, 1845, 2507, 2879, 3253
ApeKI GCWGC 8 cut(s) 1105, 1374, 1474, 2399, 2402, 2427, 3498, 3590
ApoI RAATTY 3 cut(s) 977, 1064, 1306
ArsI GACNNNNNNTTYG 4 cut(s) 251, 283, 3425, 3457
Asp700I GAANNNNTTC 2 cut(s) 2349, 2911
AspLEI GCGC 3 cut(s) 2266, 2466, 3179
AspS9I GGNCC 3 cut(s) 555, 2800, 2892
AsuC2I CCSGG 1 cut(s) 693
AsuII TTCGAA 2 cut(s) 317, 3483
AvaII GGWCC 3 cut(s) 555, 2800, 2892
BaeGI GKGCMC 1 cut(s) 2036
BalI TGGCCA 3 cut(s) 1847, 2509, 3255
BamHI GGATCC 2 cut(s) 104, 890
BanII GRGCYC 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 1631
BbsI GAAGAC 2 cut(s) 132, 1409
Bbv12I GWGCWC 2 cut(s) 1822, 2701
BbvI GCAGC 7 cut(s) 1117, 1386, 1461, 2411, 2414, 2439, 3510
BccI CCATC 8 cut(s) 656, 1186, 1292, 1535, 2039, 2546, 2657, 3293
BceAI ACGGC 1 cut(s) 1267
BcgI CGANNNNNNTGC 4 cut(s) 246, 280, 1745, 1779
BciT130I CCWGG 8 cut(s) 603, 1534, 2010, 2118, 2472, 2604, 2694, 3408
BciVI GTATCC 2 cut(s) 776, 1930
BclI TGATCA 3 cut(s) 243, 1570, 2419
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 4 cut(s) 1661, 1827, 2495, 2936
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 4 cut(s) 216, 371, 1430, 1919
BfmI CTRYAG 6 cut(s) 90, 2332, 2527, 2709, 2991, 3421
BfoI RGCGCY 1 cut(s) 2267
BfuAI ACCTGC 2 cut(s) 2452, 3116
BfuI GTATCC 2 cut(s) 776, 1930
BglII AGATCT 3 cut(s) 1426, 2764, 3317
BisI GCNGC 9 cut(s) 412, 1106, 1375, 1475, 2400, 2403, 2428, 3499, 3591
BlpI GCTNAGC 1 cut(s) 654
BlsI GCNGC 9 cut(s) 413, 1107, 1376, 1476, 2401, 2404, 2429, 3500, 3592
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 9 cut(s) 603, 693, 1534, 2010, 2118, 2472, 2604, 2694, 3408
Bme18I GGWCC 3 cut(s) 555, 2800, 2892
BmgBI CACGTC 2 cut(s) 362, 2279
BmgT120I GGNCC 3 cut(s) 555, 2800, 2892
BmrFI CCNGG 9 cut(s) 603, 693, 1534, 2010, 2118, 2472, 2604, 2694, 3408
BmrI ACTGGG 2 cut(s) 1999, 3445
BmsI GCATC 7 cut(s) 628, 839, 1094, 1425, 2033, 2840, 3095
BmuI ACTGGG 2 cut(s) 1999, 3445
BpiI GAAGAC 2 cut(s) 132, 1409
BpmI CTGGAG 4 cut(s) 586, 1060, 1516, 3061
Bpu1102I GCTNAGC 1 cut(s) 654
Bpu14I TTCGAA 2 cut(s) 317, 3483
BpuEI CTTGAG 1 cut(s) 2271
BpuMI CCSGG 1 cut(s) 693
Bsa29I ATCGAT 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 1631
BsaBI GATNNNNATC 1 cut(s) 787
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 2 cut(s) 1827, 2936
BsaJI CCNNGG 5 cut(s) 602, 1109, 1533, 3110, 3139
BsaXI ACNNNNNCTCC 6 cut(s) 1033, 1063, 1745, 1775, 1877, 1907
Bsc4I CCNNNNNNNGG 9 cut(s) 564, 602, 714, 1533, 1843, 2045, 2603, 2888, 3534
Bse3DI GCAATG 5 cut(s) 614, 1239, 2136, 2826, 3240
Bse8I GATNNNNATC 1 cut(s) 787
BseBI CCWGG 8 cut(s) 603, 1534, 2010, 2118, 2472, 2604, 2694, 3408
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 5 cut(s) 602, 1109, 1533, 3110, 3139
BseGI GGATG 4 cut(s) 391, 1207, 1552, 3553
BseJI GATNNNNATC 1 cut(s) 787
BseLI CCNNNNNNNGG 9 cut(s) 564, 602, 714, 1533, 1843, 2045, 2603, 2888, 3534
BseMI GCAATG 5 cut(s) 614, 1239, 2136, 2826, 3240
BseMII CTCAG 5 cut(s) 645, 954, 1020, 2931, 3342
BseRI GAGGAG 1 cut(s) 3381
BseSI GKGCMC 1 cut(s) 2036
BseXI GCAGC 7 cut(s) 1117, 1386, 1461, 2411, 2414, 2439, 3510
BsgI GTGCAG 2 cut(s) 25, 42
Bsh1236I CGCG 2 cut(s) 2466, 3135
Bsh1285I CGRYCG 1 cut(s) 2396
BshFI GGCC 6 cut(s) 1254, 1556, 1847, 2509, 2881, 3255
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 2396
BsiHKAI GWGCWC 2 cut(s) 1822, 2701
BsiSI CCGG 3 cut(s) 485, 693, 3372
BslFI GGGAC 5 cut(s) 527, 898, 1550, 2813, 3551
BslI CCNNNNNNNGG 9 cut(s) 564, 602, 714, 1533, 1843, 2045, 2603, 2888, 3534
BsmAI GTCTC 4 cut(s) 1661, 1827, 2495, 2936
BsmFI GGGAC 5 cut(s) 527, 898, 1550, 2813, 3551
BsmI GAATGC 2 cut(s) 477, 3458
BsnI GGCC 6 cut(s) 1254, 1556, 1847, 2509, 2881, 3255
Bso31I GGTCTC 2 cut(s) 1827, 2936
Bsp119I TTCGAA 2 cut(s) 317, 3483
Bsp1286I GDGCHC 4 cut(s) 150, 1822, 2036, 2701
Bsp1407I TGTACA 1 cut(s) 2520
Bsp1720I GCTNAGC 1 cut(s) 654
BspACI CCGC 6 cut(s) 285, 411, 1368, 2036, 2202, 3092
BspANI GGCC 6 cut(s) 1254, 1556, 1847, 2509, 2881, 3255
BspCNI CTCAG 5 cut(s) 646, 955, 1021, 2932, 3341
BspDI ATCGAT 1 cut(s) 423
BspFNI CGCG 2 cut(s) 2466, 3135
BspHI TCATGA 3 cut(s) 1564, 1882, 3565
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 2 cut(s) 2452, 3116
BspPI GGATC 8 cut(s) 99, 112, 807, 885, 898, 1965, 1967, 2462
BspQI GCTCTTC 3 cut(s) 1484, 1487, 3180
BspT104I TTCGAA 2 cut(s) 317, 3483
BspTNI GGTCTC 2 cut(s) 1827, 2936
BsrDI GCAATG 5 cut(s) 614, 1239, 2136, 2826, 3240
BsrGI TGTACA 1 cut(s) 2520
BssECI CCNNGG 5 cut(s) 602, 1109, 1533, 3110, 3139
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 3 cut(s) 1109, 3110, 3139
Bst2UI CCWGG 8 cut(s) 603, 1534, 2010, 2118, 2472, 2604, 2694, 3408
Bst6I CTCTTC 7 cut(s) 1179, 1484, 1487, 3180, 3193, 3480, 3485
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 2520
BstBAI YACGTR 1 cut(s) 1631
BstBI TTCGAA 2 cut(s) 317, 3483
BstC8I GCNNGC 6 cut(s) 287, 936, 2036, 2172, 2937, 3209
BstDEI CTNAG 7 cut(s) 654, 963, 1029, 2940, 2964, 3328, 3597
BstEII GGTNACC 1 cut(s) 325
BstF5I GGATG 4 cut(s) 391, 1207, 1552, 3553
BstFNI CGCG 2 cut(s) 2466, 3135
BstH2I RGCGCY 1 cut(s) 2267
BstHHI GCGC 3 cut(s) 2266, 2466, 3179
BstMAI GTCTC 4 cut(s) 1661, 1827, 2495, 2936
BstMCI CGRYCG 1 cut(s) 2396
BstMWI GCNNNNNNNGC 5 cut(s) 206, 472, 1111, 1374, 3112
BstNI CCWGG 8 cut(s) 603, 1534, 2010, 2118, 2472, 2604, 2694, 3408
BstNSI RCATGY 4 cut(s) 520, 1210, 1658, 3282
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 9 cut(s) 601, 691, 1532, 2008, 2116, 2470, 2602, 2692, 3406
BstSFI CTRYAG 6 cut(s) 90, 2332, 2527, 2709, 2991, 3421
BstSLI GKGCMC 1 cut(s) 2036
BstUI CGCG 2 cut(s) 2466, 3135
BstV1I GCAGC 7 cut(s) 1117, 1386, 1461, 2411, 2414, 2439, 3510
BstV2I GAAGAC 2 cut(s) 132, 1409
BstX2I RGATCY 6 cut(s) 104, 890, 1426, 1957, 2764, 3317
BstXI CCANNNNNNTGG 1 cut(s) 2224
BstYI RGATCY 6 cut(s) 104, 890, 1426, 1957, 2764, 3317
Bsu15I ATCGAT 1 cut(s) 423
BsuI GTATCC 2 cut(s) 776, 1930
BsuRI GGCC 6 cut(s) 1254, 1556, 1847, 2509, 2881, 3255
BsuTUI ATCGAT 1 cut(s) 423
BtgZI GCGATG 4 cut(s) 147, 1205, 1455, 3116
BtrI CACGTC 2 cut(s) 362, 2279
BtsCI GGATG 4 cut(s) 391, 1207, 1552, 3553
BtsI GCAGTG 2 cut(s) 2076, 2863
BveI ACCTGC 2 cut(s) 2452, 3116
Cac8I GCNNGC 6 cut(s) 287, 936, 2036, 2172, 2937, 3209
CaiI CAGNNNCTG 3 cut(s) 887, 1334, 1843
CciI TCATGA 3 cut(s) 1564, 1882, 3565
CfoI GCGC 3 cut(s) 2266, 2466, 3179
Cfr13I GGNCC 3 cut(s) 555, 2800, 2892
ClaI ATCGAT 1 cut(s) 423
CseI GACGC 1 cut(s) 3124
CsiI ACCWGGT 2 cut(s) 2692, 3406
DdeI CTNAG 7 cut(s) 654, 963, 1029, 2940, 2964, 3328, 3597
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 3509
DseDI GACNNNNNNGTC 1 cut(s) 3509
EaeI YGGCCR 4 cut(s) 1252, 1845, 2507, 3253
Eam1104I CTCTTC 7 cut(s) 1179, 1484, 1487, 3180, 3193, 3480, 3485
EarI CTCTTC 7 cut(s) 1179, 1484, 1487, 3180, 3193, 3480, 3485
EciI GGCGGA 1 cut(s) 1383
Eco130I CCWWGG 3 cut(s) 1109, 3110, 3139
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 1827, 2936
Eco47I GGWCC 3 cut(s) 555, 2800, 2892
Eco57I CTGAAG 3 cut(s) 1421, 2516, 3294
Eco72I CACGTG 1 cut(s) 1631
Eco91I GGTNACC 1 cut(s) 325
EcoO109I RGGNCCY 2 cut(s) 555, 2892
EcoO65I GGTNACC 1 cut(s) 325
EcoRII CCWGG 8 cut(s) 601, 1532, 2008, 2116, 2470, 2602, 2692, 3406
EcoT14I CCWWGG 3 cut(s) 1109, 3110, 3139
EcoT22I ATGCAT 1 cut(s) 2026
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 1109, 3110, 3139
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 1535, 1567
FaqI GGGAC 5 cut(s) 527, 898, 1550, 2813, 3551
FauI CCCGC 3 cut(s) 278, 2043, 2195
FauNDI CATATG 1 cut(s) 2773
FbaI TGATCA 3 cut(s) 243, 1570, 2419
FblI GTMKAC 4 cut(s) 508, 2577, 3237, 3513
Fnu4HI GCNGC 9 cut(s) 412, 1106, 1375, 1475, 2400, 2403, 2428, 3499, 3591
FokI GGATG 4 cut(s) 398, 1214, 1559, 3560
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 9 cut(s) 412, 1106, 1375, 1475, 2400, 2403, 2428, 3499, 3591
FspBI CTAG 4 cut(s) 216, 371, 1430, 1919
GlaI GCGC 3 cut(s) 2265, 2465, 3178
GluI GCNGC 9 cut(s) 412, 1106, 1375, 1475, 2400, 2403, 2428, 3499, 3591
GsuI CTGGAG 4 cut(s) 586, 1060, 1516, 3061
HaeII RGCGCY 1 cut(s) 2267
HaeIII GGCC 6 cut(s) 1254, 1556, 1847, 2509, 2881, 3255
HapII CCGG 3 cut(s) 485, 693, 3372
HgaI GACGC 1 cut(s) 3124
HhaI GCGC 3 cut(s) 2266, 2466, 3179
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 3 cut(s) 2264, 2464, 3177
HinP1I GCGC 3 cut(s) 2264, 2464, 3177
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 3 cut(s) 530, 1214, 3205
HpaII CCGG 3 cut(s) 485, 693, 3372
Hpy99I CGWCG 1 cut(s) 3506
HpyAV CCTTC 8 cut(s) 340, 904, 1040, 1191, 1501, 1920, 1986, 3532
HpyCH4IV ACGT 8 cut(s) 129, 361, 1630, 2278, 2623, 2872, 2955, 3149
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 472, 1111, 1374, 3112
HpyF3I CTNAG 7 cut(s) 654, 963, 1029, 2940, 2964, 3328, 3597
HpySE526I ACGT 8 cut(s) 129, 361, 1630, 2278, 2623, 2872, 2955, 3149
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 3 cut(s) 2264, 2464, 3177
Ksp22I TGATCA 3 cut(s) 243, 1570, 2419
LguI GCTCTTC 3 cut(s) 1484, 1487, 3180
LmnI GCTCC 9 cut(s) 352, 463, 567, 820, 1413, 1753, 2568, 3055, 3368
Lsp1109I GCAGC 7 cut(s) 1117, 1386, 1461, 2411, 2414, 2439, 3510
LweI GCATC 7 cut(s) 628, 839, 1094, 1425, 2033, 2840, 3095
MabI ACCWGGT 2 cut(s) 2692, 3406
MaeI CTAG 4 cut(s) 216, 371, 1430, 1919
MaeII ACGT 8 cut(s) 129, 361, 1630, 2278, 2623, 2872, 2955, 3149
MflI RGATCY 6 cut(s) 104, 890, 1426, 1957, 2764, 3317
MhlI GDGCHC 4 cut(s) 150, 1822, 2036, 2701
MlsI TGGCCA 3 cut(s) 1847, 2509, 3255
MluNI TGGCCA 3 cut(s) 1847, 2509, 3255
MlyI GAGTC 6 cut(s) 620, 1880, 1945, 2007, 2056, 3512
MmeI TCCRAC 3 cut(s) 1975, 3343, 3392
Mox20I TGGCCA 3 cut(s) 1847, 2509, 3255
Mph1103I ATGCAT 1 cut(s) 2026
MroXI GAANNNNTTC 2 cut(s) 2349, 2911
MscI TGGCCA 3 cut(s) 1847, 2509, 3255
MseI TTAA 4 cut(s) 99, 171, 455, 2732
MslI CAYNNNNRTG 4 cut(s) 2029, 2071, 2871, 3267
Msp20I TGGCCA 3 cut(s) 1847, 2509, 3255
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 3 cut(s) 485, 693, 3372
MspR9I CCNGG 9 cut(s) 603, 693, 1534, 2010, 2118, 2472, 2604, 2694, 3408
Mva1269I GAATGC 2 cut(s) 477, 3458
MvaI CCWGG 8 cut(s) 603, 1534, 2010, 2118, 2472, 2604, 2694, 3408
MvnI CGCG 2 cut(s) 2466, 3135
MwoI GCNNNNNNNGC 5 cut(s) 206, 472, 1111, 1374, 3112
NciI CCSGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 2773
NmuCI GTSAC 7 cut(s) 34, 325, 397, 638, 1948, 2639, 2726
NsiI ATGCAT 1 cut(s) 2026
NspI RCATGY 4 cut(s) 520, 1210, 1658, 3282
NspV TTCGAA 2 cut(s) 317, 3483
OliI CACNNNNGTG 1 cut(s) 2871
PagI TCATGA 3 cut(s) 1564, 1882, 3565
PaqCI CACCTGC 1 cut(s) 2452
PciI ACATGT 1 cut(s) 516
PciSI GCTCTTC 3 cut(s) 1484, 1487, 3180
PctI GAATGC 2 cut(s) 477, 3458
PdmI GAANNNNTTC 2 cut(s) 2349, 2911
PfeI GAWTC 7 cut(s) 911, 1517, 1777, 1852, 2612, 2885, 2912
PflMI CCANNNNNTGG 3 cut(s) 1843, 2045, 2603
PfoI TCCNGGA 2 cut(s) 2008, 2602
PkrI GCNGC 9 cut(s) 413, 1107, 1376, 1476, 2401, 2404, 2429, 3500, 3592
Ple19I CGATCG 1 cut(s) 2396
PleI GAGTC 6 cut(s) 619, 1880, 1945, 2006, 2056, 3512
PmaCI CACGTG 1 cut(s) 1631
PmlI CACGTG 1 cut(s) 1631
PpsI GAGTC 6 cut(s) 619, 1880, 1945, 2006, 2056, 3512
Ppu21I YACGTR 1 cut(s) 1631
PpuMI RGGWCCY 2 cut(s) 555, 2892
PscI ACATGT 1 cut(s) 516
PsiI TTATAA 1 cut(s) 2162
Psp5II RGGWCCY 2 cut(s) 555, 2892
Psp6I CCWGG 8 cut(s) 601, 1532, 2008, 2116, 2470, 2602, 2692, 3406
PspCI CACGTG 1 cut(s) 1631
PspEI GGTNACC 1 cut(s) 325
PspGI CCWGG 8 cut(s) 601, 1532, 2008, 2116, 2470, 2602, 2692, 3406
PspPI GGNCC 3 cut(s) 555, 2800, 2892
PspPPI RGGWCCY 2 cut(s) 555, 2892
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 3 cut(s) 887, 1334, 1843
PsuI RGATCY 6 cut(s) 104, 890, 1426, 1957, 2764, 3317
PvuI CGATCG 1 cut(s) 2396
PvuII CAGCTG 1 cut(s) 938
RseI CAYNNNNRTG 4 cut(s) 2029, 2071, 2871, 3267
SapI GCTCTTC 3 cut(s) 1484, 1487, 3180
SaqAI TTAA 4 cut(s) 99, 171, 455, 2732
SatI GCNGC 9 cut(s) 412, 1106, 1375, 1475, 2400, 2403, 2428, 3499, 3591
Sau96I GGNCC 3 cut(s) 555, 2800, 2892
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 6 cut(s) 620, 1880, 1945, 2007, 2056, 3512
ScrFI CCNGG 9 cut(s) 603, 693, 1534, 2010, 2118, 2472, 2604, 2694, 3408
SduI GDGCHC 4 cut(s) 150, 1822, 2036, 2701
SexAI ACCWGGT 2 cut(s) 2692, 3406
SfaNI GCATC 7 cut(s) 628, 839, 1094, 1425, 2033, 2840, 3095
SfcI CTRYAG 6 cut(s) 90, 2332, 2527, 2709, 2991, 3421
SfuI TTCGAA 2 cut(s) 317, 3483
SinI GGWCC 3 cut(s) 555, 2800, 2892
SmiMI CAYNNNNRTG 4 cut(s) 2029, 2071, 2871, 3267
SmlI CTYRAG 1 cut(s) 2286
SmoI CTYRAG 1 cut(s) 2286
SpeI ACTAGT 1 cut(s) 215
SsiI CCGC 6 cut(s) 285, 411, 1368, 2036, 2202, 3092
SspI AATATT 2 cut(s) 1317, 2981
SspMI CTAG 4 cut(s) 216, 371, 1430, 1919
StyD4I CCNGG 9 cut(s) 601, 691, 1532, 2008, 2116, 2470, 2602, 2692, 3406
StyI CCWWGG 3 cut(s) 1109, 3110, 3139
TaiI ACGT 8 cut(s) 132, 364, 1633, 2281, 2626, 2875, 2958, 3152
TaqI TCGA 8 cut(s) 238, 317, 423, 469, 622, 1482, 1765, 3483
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 7 cut(s) 911, 1517, 1777, 1852, 2612, 2885, 2912
Tru1I TTAA 4 cut(s) 99, 171, 455, 2732
Tru9I TTAA 4 cut(s) 99, 171, 455, 2732
TseFI GTSAC 7 cut(s) 34, 325, 397, 638, 1948, 2639, 2726
TseI GCWGC 8 cut(s) 1105, 1374, 1474, 2399, 2402, 2427, 3498, 3590
Tsp45I GTSAC 7 cut(s) 34, 325, 397, 638, 1948, 2639, 2726
TspGWI ACGGA 4 cut(s) 1356, 2919, 3068, 3560
Van91I CCANNNNNTGG 3 cut(s) 1843, 2045, 2603
VpaK11BI GGWCC 3 cut(s) 555, 2800, 2892
XapI RAATTY 3 cut(s) 977, 1064, 1306
XbaI TCTAGA 1 cut(s) 1429
XceI RCATGY 4 cut(s) 520, 1210, 1658, 3282
XcmI CCANNNNNNNNNTGG 3 cut(s) 1358, 2361, 3359
XmiI GTMKAC 4 cut(s) 508, 2577, 3237, 3513
XmnI GAANNNNTTC 2 cut(s) 2349, 2911
XspI CTAG 4 cut(s) 216, 371, 1430, 1919
ZraI GACGTC 1 cut(s) 130
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 2026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.