Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001351

Physical Location & Seq

Genomic Coordinates Forward (+)

28606916 .. 28612194

Gene Structure 5279 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1142300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGGAGAGCTTCTGCAGCTTCCTGTCTTTGAAGGCCGCGTTCTTATTGATTTTCTTAGTGAATTCCTCAGCAGGTCATCAACATTACAGGAACAAGGAGGGAGGTGATGGAGTGCAGCCACTTTCCAAGATTTTAATCCACAAAACCGTGAACGCGATTCACGAGAAGGCTTCCGTTCATGCGTCCCCTCTACTTCTCGGCACCACGGGGAAGGATTATGAATGGGTAACTGTGACTGTTGTTAGTCCTAATCCTTCTGCTAATGACTGGTTGGGAGTCTTTTCTCCAGCAAAATTCAATGCATCAAGTTGTCCATTCACAGAAGATTACTATGAGCCATATCTGTGCACAGCTCCTATCAAGTACAAATATGCGAACCACTCCAATGCCAATTACAACAAGACAGGCAAAAACACTCTGTATTTCCGGTTGATCAATCAACGCTCGGATTTCGCATTTGCATTGTTCTCCGGCGGGCTGTCAAGCCCGAAATTAGTGGCAATTTCTAATGCTATAGTTTTCGCAAATCCAAAGGCGCCTCTTTACCCTCGTCTTGCTCTAGGGAAGCACTGGGATGAAATGACAGTAACCTGGACAAGCGGCTACGACATCAGTGAAGCTGTACCATTTGTCGAGTGGGGTTTCGCGGGAGAAGCTCAAACTCGATCCCCAGCAGGAACATTGACTTTCCCTCGAGGAAGCATGTGTGATGAACCAGCACGAACAGTTGGTTGGCGCGATCCTGGTTTCTTCCATACAAGTTTTTTGAAAAATTTGTGGCCTAATTCAAAGTACACTTACAAGCTTGGTCATAGGTTAAACACTGGTTCCTACATCTGGAGCAAGACCTACAATTTTACGTCATCCCCGTATCCAGGACAGGACTCGTTACAGAAGGTTATCATATTCGGTGACATGGGAAAGGCTGAGCGTGATGGTTCAAATGAATACGCTGATTACCAGCCCGGGGCACTCCTTACCACAGATGCACTGATCAAGGACCTGGATAACATTGACATAGTCTTTCACATTGGGGATATGCCCTATGCCAATGGATATATCTCACAGTGGGACCAGTTTACATCACAGGTGGAGCCCATTGCATCAGCTGTGCCTTATATGGTTGCAAGCGGCAATCATGAACGAACTACACCAAACACCGGAGGCTTCTATGACGTAAATGATTCAGGTGGTGAATGTGGTGTGCCGGCTGAGACCATGTTTTTTTTTCCTGCTGAGAACAGAGCCAAGTTCTGGTATCAAACAGACTATGGAATGTTCCACTTCTGCATAGCAGACACTGAGCATGATTGGAGGGAAGGTTCTGAGCAGTACAGATTCATTGAGAAGTGCCTTGCAGCAGCTGACAGGAACAAACAGCCTTGGCTGATATTCGCTGCTCATCGTGTTCTCGGTTATTCCTCCAACTCCTGGTATGGCCAGGAGGGTCAATTTTCAGAGCCTATGGGAAGAGATGACATGCAAAAGCTCTGGCAAAGGTACAAGGTGGACATTGCATTTTTTGGCCATGTCCATAACTATGAAAGAATATGCCCCATTTATCAGAATCAATGTGTGAATTCGGAAATCTCTCACTATTCGGGGACGGTGAACGGAACAATCCATGTAGTTGTTGGAGGAGGAGGCAGCCACTTATCGGCTTTCAGCACACTGAAGACAGTTTGGAGTGTTTACCAAGATCTGGACTTTGGGTACACGAAACTCACAGCATTCAACCACTCATCACTTCTGTTTGAGTACATGAAGAGCAGTGATGGCAAGGTCTATGATTCCTTCACAATCTCAAGAGATTACAGAGATGTTTTGGCCTGTGTTCATGATGGCTGTGAACCAACCACTTTGGCTACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005618 GO:0005623 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030312 GO:0042578 GO:0044237 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

624

Amino Acids

69.96

Weight (kDa)

5.95

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
fn3_PAP	PF17808	59 - 176	1.6e-43	Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N	PF16656	183 - 287	3e-12	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	299 - 514	1.7e-16	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	539 - 598	9.1e-16	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24890 AT5G50400 AT5G50400
fragaria_vesca	FvH4_5g08240 FvH4_5g08241 FvH4_5g08282 FvH4_5g08283 FvH4_5g08284
malus_domestica	MD06G1142200.v1.1 MD06G1142300.v1.1 MD06G1142400.v1.1 MD14G1157600.v1.1
prunus_persica	Prupe.5G151400_v2.0.a1 Prupe.5G151500_v2.0.a1 Prupe.5G151600_v2.0.a1
pyrus_communis	pycom06g13200 pycom06g13210 pycom06g13220
rosa_chinensis	RchiOBHm_Chr7g0190231 RchiOBHm_Chr7g0190241 RchiOBHm_Chr7g0190251 RchiOBHm_Chr7g0190261 RchiOBHm_Chr7g0190281
rosa_laevigata	RLG00000004547 RLG00000004549 RLG00000004550 RLG00000004551
rosa_multiflora	Rmu_sc0004886.1_g000021 Rmu_sc0005487.1_g000006 Rmu_sc0031901.1_g000001 Rmu_sc0031902.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264770
rosa_rugosa	Rorug06G0503100.1 Rorug06G0503300 Rorug06G0503400
rosa_samantha	Rh7AG108500 Rh7AG108800 Rh7AG108900 Rh7AG109100 Rh7BG111100 Rh7BG111300 Rh7BG111400 Rh7BG111500 Rh7CG113500 Rh7CG113700 Rh7CG113800 Rh7CG113900 Rh7DG112100
rosa_wichuraiana	Rw7G009410 Rw7G009420 Rw7G009440 Rw7G009450 Rw7G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	693
Acc36I	ACCTGC	1 cut(s)	62
AccB1I	GGYRCC	2 cut(s)	200, 535
AccB7I	CCANNNNNTGG	3 cut(s)	1254, 1431, 1702
AccII	CGCG	4 cut(s)	38, 155, 647, 738
AciI	CCGC	5 cut(s)	36, 474, 600, 647, 1131
AclWI	GGATC	2 cut(s)	660, 734
AcoI	YGGCCR	2 cut(s)	1438, 1525
AcsI	RAATTY	4 cut(s)	61, 293, 772, 1579
AcuI	CTGAAG	1 cut(s)	1694
AcyI	GRCGYC	1 cut(s)	536
AfaI	GTAC	7 cut(s)	365, 624, 794, 1334, 1502, 1715, 1760
AfiI	CCNNNNNNNGG	8 cut(s)	837, 875, 967, 1160, 1254, 1431, 1657, 1702
AgsI	TTSAA	6 cut(s)	31, 298, 769, 789, 942, 1735
AjnI	CCWGG	6 cut(s)	590, 742, 874, 1002, 1430, 1440
AjuI	GAANNNNNNNTTGG	2 cut(s)	715, 747
AloI	GAACNNNNNNTCC	2 cut(s)	1306, 1338
AluBI	AGCT	9 cut(s)	9, 18, 353, 620, 656, 805, 1109, 1364, 1489
AluI	AGCT	9 cut(s)	9, 18, 353, 620, 656, 805, 1109, 1364, 1489
Alw21I	GWGCWC	1 cut(s)	350
Alw26I	GTCTC	1 cut(s)	1208
Alw44I	GTGCAC	1 cut(s)	346
AlwI	GGATC	2 cut(s)	660, 734
AlwNI	CAGNNNCTG	2 cut(s)	1301, 1364
Ama87I	CYCGRG	2 cut(s)	693, 965
AoxI	GGCC	5 cut(s)	33, 779, 1438, 1525, 1827
ApaLI	GTGCAC	1 cut(s)	346
ApeKI	GCWGC	6 cut(s)	15, 115, 1358, 1361, 1397, 1647
ApoI	RAATTY	4 cut(s)	61, 293, 772, 1579
AspLEI	GCGC	2 cut(s)	538, 738
AspS9I	GGNCC	2 cut(s)	1000, 1072
AsuC2I	CCSGG	2 cut(s)	966, 967
AsuHPI	GGTGA	4 cut(s)	116, 923, 1205, 1621
AvaI	CYCGRG	2 cut(s)	693, 965
AvaII	GGWCC	2 cut(s)	1000, 1072
BaeGI	GKGCMC	2 cut(s)	350, 973
BalI	TGGCCA	2 cut(s)	1440, 1527
BanI	GGYRCC	2 cut(s)	200, 535
BanII	GRGCYC	1 cut(s)	1098
BauI	CACGAG	1 cut(s)	161
BbsI	GAAGAC	1 cut(s)	1682
Bbv12I	GWGCWC	1 cut(s)	350
BbvCI	CCTCAGC	1 cut(s)	67
BbvI	GCAGC	6 cut(s)	27, 127, 1370, 1373, 1384, 1659
BccI	CCATC	4 cut(s)	101, 929, 1769, 1835
BciT130I	CCWGG	6 cut(s)	592, 744, 876, 1004, 1432, 1442
BciVI	GTATCC	1 cut(s)	882
BclI	TGATCA	2 cut(s)	432, 993
BcnI	CCSGG	2 cut(s)	966, 967
BcoDI	GTCTC	1 cut(s)	1208
BfaI	CTAG	1 cut(s)	560
BfmI	CTRYAG	2 cut(s)	13, 513
BfoI	RGCGCY	1 cut(s)	539
BfuAI	ACCTGC	1 cut(s)	62
BfuI	GTATCC	1 cut(s)	882
BglII	AGATCT	1 cut(s)	1699
BisI	GCNGC	9 cut(s)	16, 36, 116, 601, 1132, 1359, 1362, 1398, 1648
BlpI	GCTNAGC	1 cut(s)	927
BlsI	GCNGC	9 cut(s)	17, 37, 117, 602, 1133, 1360, 1363, 1399, 1649
Bme1390I	CCNGG	8 cut(s)	592, 744, 876, 966, 967, 1004, 1432, 1442
Bme18I	GGWCC	2 cut(s)	1000, 1072
BmeT110I	CYCGRG	2 cut(s)	693, 965
BmgT120I	GGNCC	2 cut(s)	1000, 1072
BmiI	GGNNCC	5 cut(s)	202, 537, 829, 1073, 1095
BmrFI	CCNGG	8 cut(s)	592, 744, 876, 966, 967, 1004, 1432, 1442
BmrI	ACTGGG	1 cut(s)	580
BmsI	GCATC	3 cut(s)	311, 976, 1112
BmuI	ACTGGG	1 cut(s)	580
BpiI	GAAGAC	1 cut(s)	1682
BpmI	CTGGAG	2 cut(s)	270, 859
Bpu10I	CCTNAGC	1 cut(s)	67
Bpu1102I	GCTNAGC	1 cut(s)	927
BpuEI	CTTGAG	1 cut(s)	1789
BpuMI	CCSGG	2 cut(s)	966, 967
BsaAI	YACGTR	1 cut(s)	1869
BsaBI	GATNNNNATC	1 cut(s)	134
BsaHI	GRCGYC	1 cut(s)	536
BsaI	GGTCTC	1 cut(s)	1208
BsaJI	CCNNGG	4 cut(s)	204, 965, 966, 1382
BsaWI	WCCGGW	2 cut(s)	426, 1160
BsaXI	ACNNNNNCTCC	2 cut(s)	1306, 1336
Bsc4I	CCNNNNNNNGG	8 cut(s)	837, 875, 967, 1160, 1254, 1431, 1657, 1702
Bse118I	RCCGGY	1 cut(s)	1207
Bse1I	ACTGG	4 cut(s)	272, 575, 829, 1075
Bse3DI	GCAATG	2 cut(s)	1098, 1512
Bse8I	GATNNNNATC	1 cut(s)	134
BseBI	CCWGG	6 cut(s)	592, 744, 876, 1004, 1432, 1442
BseDI	CCNNGG	4 cut(s)	204, 965, 966, 1382
BseGI	GGATG	2 cut(s)	580, 863
BseJI	GATNNNNATC	1 cut(s)	134
BseLI	CCNNNNNNNGG	8 cut(s)	837, 875, 967, 1160, 1254, 1431, 1657, 1702
BseMI	GCAATG	2 cut(s)	1098, 1512
BseMII	CTCAG	6 cut(s)	81, 918, 1203, 1227, 1293, 1317
BseNI	ACTGG	4 cut(s)	272, 575, 829, 1075
BseRI	GAGGAG	2 cut(s)	1653, 1656
BseSI	GKGCMC	2 cut(s)	350, 973
BseXI	GCAGC	6 cut(s)	27, 127, 1370, 1373, 1384, 1659
BseYI	CCCAGC	1 cut(s)	670
BsgI	GTGCAG	1 cut(s)	134
Bsh1236I	CGCG	4 cut(s)	38, 155, 647, 738
BshFI	GGCC	5 cut(s)	35, 781, 1440, 1527, 1829
BshNI	GGYRCC	2 cut(s)	200, 535
BsiHKAI	GWGCWC	1 cut(s)	350
BsiHKCI	CYCGRG	2 cut(s)	693, 965
BsiSI	CCGG	5 cut(s)	427, 471, 966, 1161, 1208
BslFI	GGGAC	3 cut(s)	169, 1085, 1618
BslI	CCNNNNNNNGG	8 cut(s)	837, 875, 967, 1160, 1254, 1431, 1657, 1702
BsmAI	GTCTC	1 cut(s)	1208
BsmFI	GGGAC	3 cut(s)	169, 1085, 1618
BsmI	GAATGC	1 cut(s)	1730
BsnI	GGCC	5 cut(s)	35, 781, 1440, 1527, 1829
Bso31I	GGTCTC	1 cut(s)	1208
BsoBI	CYCGRG	2 cut(s)	693, 965
Bsp1286I	GDGCHC	3 cut(s)	350, 973, 1098
Bsp143I	GATC	5 cut(s)	432, 665, 739, 993, 1699
Bsp1720I	GCTNAGC	1 cut(s)	927
BspACI	CCGC	5 cut(s)	36, 474, 600, 647, 1131
BspANI	GGCC	5 cut(s)	35, 781, 1440, 1527, 1829
BspCNI	CTCAG	6 cut(s)	80, 919, 1204, 1228, 1294, 1318
BspFNI	CGCG	4 cut(s)	38, 155, 647, 738
BspHI	TCATGA	2 cut(s)	1138, 1837
BspLI	GGNNCC	5 cut(s)	202, 537, 829, 1073, 1095
BspMAI	CTGCAG	1 cut(s)	17
BspMI	ACCTGC	1 cut(s)	62
BspPI	GGATC	2 cut(s)	660, 734
BspQI	GCTCTTC	1 cut(s)	1760
BspT107I	GGYRCC	2 cut(s)	200, 535
BspTNI	GGTCTC	1 cut(s)	1208
BsrDI	GCAATG	2 cut(s)	1098, 1512
BsrFI	RCCGGY	1 cut(s)	1207
BsrI	ACTGG	4 cut(s)	272, 575, 829, 1075
BssAI	RCCGGY	1 cut(s)	1207
BssECI	CCNNGG	4 cut(s)	204, 965, 966, 1382
BssMI	GATC	5 cut(s)	432, 665, 739, 993, 1699
BssNI	GRCGYC	1 cut(s)	536
BssSI	CACGAG	1 cut(s)	161
BssT1I	CCWWGG	1 cut(s)	1382
Bst2BI	CACGAG	1 cut(s)	161
Bst2UI	CCWGG	6 cut(s)	592, 744, 876, 1004, 1432, 1442
Bst4CI	ACNGT	8 cut(s)	148, 232, 238, 586, 727, 1068, 1609, 1681
Bst6I	CTCTTC	2 cut(s)	1465, 1760
BstACI	GRCGYC	1 cut(s)	536
BstBAI	YACGTR	1 cut(s)	1869
BstC8I	GCNNGC	3 cut(s)	476, 1129, 1209
BstDEI	CTNAG	7 cut(s)	55, 67, 927, 1212, 1236, 1302, 1326
BstDSI	CCRYGG	1 cut(s)	204
BstF5I	GGATG	2 cut(s)	580, 863
BstFNI	CGCG	4 cut(s)	38, 155, 647, 738
BstH2I	RGCGCY	1 cut(s)	539
BstHHI	GCGC	2 cut(s)	538, 738
BstKTI	GATC	5 cut(s)	435, 668, 742, 996, 1702
BstMAI	GTCTC	1 cut(s)	1208
BstMBI	GATC	5 cut(s)	432, 665, 739, 993, 1699
BstMWI	GCNNNNNNNGC	3 cut(s)	15, 653, 1776
BstNI	CCWGG	6 cut(s)	592, 744, 876, 1004, 1432, 1442
BstNSI	RCATGY	2 cut(s)	706, 1483
BstSCI	CCNGG	8 cut(s)	590, 742, 874, 964, 965, 1002, 1430, 1440
BstSFI	CTRYAG	2 cut(s)	13, 513
BstSLI	GKGCMC	2 cut(s)	350, 973
BstSNI	TACGTA	1 cut(s)	1869
BstUI	CGCG	4 cut(s)	38, 155, 647, 738
BstV1I	GCAGC	6 cut(s)	27, 127, 1370, 1373, 1384, 1659
BstV2I	GAAGAC	1 cut(s)	1682
BstX2I	RGATCY	1 cut(s)	1699
BstYI	RGATCY	1 cut(s)	1699
BsuI	GTATCC	1 cut(s)	882
BsuRI	GGCC	5 cut(s)	35, 781, 1440, 1527, 1829
BtgI	CCRYGG	1 cut(s)	204
BtsCI	GGATG	2 cut(s)	580, 863
BtsI	GCAGTG	1 cut(s)	1777
BtsIMutI	CAGTG	8 cut(s)	568, 619, 822, 989, 1073, 1299, 1670, 1777
BveI	ACCTGC	1 cut(s)	62
Cac8I	GCNNGC	3 cut(s)	476, 1129, 1209
CaiI	CAGNNNCTG	2 cut(s)	1301, 1364
CciI	TCATGA	2 cut(s)	1138, 1837
CfoI	GCGC	2 cut(s)	538, 738
Cfr10I	RCCGGY	1 cut(s)	1207
Cfr13I	GGNCC	2 cut(s)	1000, 1072
Cfr9I	CCCGGG	1 cut(s)	965
CseI	GACGC	1 cut(s)	171
Csp6I	GTAC	7 cut(s)	364, 623, 793, 1333, 1501, 1714, 1759
CviQI	GTAC	7 cut(s)	364, 623, 793, 1333, 1501, 1714, 1759
DdeI	CTNAG	7 cut(s)	55, 67, 927, 1212, 1236, 1302, 1326
DinI	GGCGCC	1 cut(s)	537
DpnI	GATC	5 cut(s)	434, 667, 741, 995, 1701
DpnII	GATC	5 cut(s)	432, 665, 739, 993, 1699
EaeI	YGGCCR	2 cut(s)	1438, 1525
Eam1104I	CTCTTC	2 cut(s)	1465, 1760
EarI	CTCTTC	2 cut(s)	1465, 1760
Eco105I	TACGTA	1 cut(s)	1869
Eco130I	CCWWGG	1 cut(s)	1382
Eco24I	GRGCYC	1 cut(s)	1098
Eco31I	GGTCTC	1 cut(s)	1208
Eco47I	GGWCC	2 cut(s)	1000, 1072
Eco57I	CTGAAG	1 cut(s)	1694
Eco88I	CYCGRG	2 cut(s)	693, 965
EcoO109I	RGGNCCY	1 cut(s)	1000
EcoRI	GAATTC	2 cut(s)	61, 1579
EcoRII	CCWGG	6 cut(s)	590, 742, 874, 1002, 1430, 1440
EcoT14I	CCWWGG	1 cut(s)	1382
EcoT22I	ATGCAT	1 cut(s)	304
EcoT38I	GRGCYC	1 cut(s)	1098
EgeI	GGCGCC	1 cut(s)	537
EheI	GGCGCC	1 cut(s)	537
ErhI	CCWWGG	1 cut(s)	1382
FaqI	GGGAC	3 cut(s)	169, 1085, 1618
FauI	CCCGC	2 cut(s)	467, 640
FbaI	TGATCA	2 cut(s)	432, 993
Fnu4HI	GCNGC	9 cut(s)	16, 36, 116, 601, 1132, 1359, 1362, 1398, 1648
FokI	GGATG	2 cut(s)	587, 850
FriOI	GRGCYC	1 cut(s)	1098
Fsp4HI	GCNGC	9 cut(s)	16, 36, 116, 601, 1132, 1359, 1362, 1398, 1648
FspBI	CTAG	1 cut(s)	560
GlaI	GCGC	2 cut(s)	537, 737
GluI	GCNGC	9 cut(s)	16, 36, 116, 601, 1132, 1359, 1362, 1398, 1648
GsaI	CCCAGC	1 cut(s)	674
GsuI	CTGGAG	2 cut(s)	270, 859
HaeII	RGCGCY	1 cut(s)	539
HaeIII	GGCC	5 cut(s)	35, 781, 1440, 1527, 1829
HapII	CCGG	5 cut(s)	427, 471, 966, 1161, 1208
HgaI	GACGC	1 cut(s)	171
HhaI	GCGC	2 cut(s)	538, 738
Hin1I	GRCGYC	1 cut(s)	536
Hin6I	GCGC	2 cut(s)	536, 736
HinP1I	GCGC	2 cut(s)	536, 736
HindIII	AAGCTT	1 cut(s)	803
HinfI	GANTC	7 cut(s)	157, 276, 884, 1184, 1338, 1567, 1790
HpaII	CCGG	5 cut(s)	427, 471, 966, 1161, 1208
HphI	GGTGA	4 cut(s)	116, 923, 1205, 1621
Hpy166II	GTNNAC	9 cut(s)	151, 348, 795, 1080, 1510, 1612, 1693, 1716, 1850
Hpy188I	TCNGA	5 cut(s)	448, 1327, 1459, 1566, 1585
Hpy188III	TCNNGA	6 cut(s)	161, 838, 1139, 1703, 1806, 1838
Hpy8I	GTNNAC	9 cut(s)	151, 348, 795, 1080, 1510, 1612, 1693, 1716, 1850
HpyAV	CCTTC	7 cut(s)	25, 160, 205, 264, 889, 1313, 1804
HpyCH4III	ACNGT	8 cut(s)	148, 232, 238, 586, 727, 1068, 1609, 1681
HpyCH4IV	ACGT	3 cut(s)	860, 1176, 1868
HpyF10VI	GCNNNNNNNGC	3 cut(s)	15, 653, 1776
HpyF3I	CTNAG	7 cut(s)	55, 67, 927, 1212, 1236, 1302, 1326
HpySE526I	ACGT	3 cut(s)	860, 1176, 1868
Hsp92I	GRCGYC	1 cut(s)	536
HspAI	GCGC	2 cut(s)	536, 736
KasI	GGCGCC	1 cut(s)	535
KroI	GCCGGC	1 cut(s)	1207
KroNI	GCCGGC	1 cut(s)	1209
Ksp22I	TGATCA	2 cut(s)	432, 993
Kzo9I	GATC	5 cut(s)	432, 665, 739, 993, 1699
LguI	GCTCTTC	1 cut(s)	1760
LmnI	GCTCC	3 cut(s)	358, 840, 1093
Lsp1109I	GCAGC	6 cut(s)	27, 127, 1370, 1373, 1384, 1659
LweI	GCATC	3 cut(s)	311, 976, 1112
MaeI	CTAG	1 cut(s)	560
MaeII	ACGT	3 cut(s)	860, 1176, 1868
MaeIII	GTNAC	5 cut(s)	226, 232, 586, 888, 911
MalI	GATC	5 cut(s)	434, 667, 741, 995, 1701
MboI	GATC	5 cut(s)	432, 665, 739, 993, 1699
MboII	GAAGA	5 cut(s)	335, 742, 1482, 1687, 1777
MflI	RGATCY	1 cut(s)	1699
MhlI	GDGCHC	3 cut(s)	350, 973, 1098
MlsI	TGGCCA	2 cut(s)	1440, 1527
MluNI	TGGCCA	2 cut(s)	1440, 1527
Mly113I	GGCGCC	1 cut(s)	536
MlyI	GAGTC	2 cut(s)	285, 878
MmeI	TCCRAC	2 cut(s)	1449, 1615
Mox20I	TGGCCA	2 cut(s)	1440, 1527
Mph1103I	ATGCAT	1 cut(s)	304
MroNI	GCCGGC	1 cut(s)	1207
MscI	TGGCCA	2 cut(s)	1440, 1527
MseI	TTAA	2 cut(s)	134, 818
MslI	CAYNNNNRTG	4 cut(s)	343, 384, 573, 1539
Msp20I	TGGCCA	2 cut(s)	1440, 1527
MspA1I	CMGCKG	2 cut(s)	1109, 1364
MspI	CCGG	5 cut(s)	427, 471, 966, 1161, 1208
MspR9I	CCNGG	8 cut(s)	592, 744, 876, 966, 967, 1004, 1432, 1442
Mva1269I	GAATGC	1 cut(s)	1730
MvaI	CCWGG	6 cut(s)	592, 744, 876, 1004, 1432, 1442
MvnI	CGCG	4 cut(s)	38, 155, 647, 738
MwoI	GCNNNNNNNGC	3 cut(s)	15, 653, 1776
NaeI	GCCGGC	1 cut(s)	1209
NarI	GGCGCC	1 cut(s)	536
NciI	CCSGG	2 cut(s)	966, 967
NdeII	GATC	5 cut(s)	432, 665, 739, 993, 1699
NgoMIV	GCCGGC	1 cut(s)	1207
NlaIV	GGNNCC	5 cut(s)	202, 537, 829, 1073, 1095
NmeAIII	GCCGAG	1 cut(s)	177
NmuCI	GTSAC	2 cut(s)	232, 911
NsiI	ATGCAT	1 cut(s)	304
NspI	RCATGY	2 cut(s)	706, 1483
PaeR7I	CTCGAG	1 cut(s)	693
PagI	TCATGA	2 cut(s)	1138, 1837
PciSI	GCTCTTC	1 cut(s)	1760
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 866
PctI	GAATGC	1 cut(s)	1730
PdiI	GCCGGC	1 cut(s)	1209
PfeI	GAWTC	5 cut(s)	157, 1184, 1338, 1567, 1790
PflFI	GACNNNGTC	1 cut(s)	1019
PflMI	CCANNNNNTGG	3 cut(s)	1254, 1431, 1702
PfoI	TCCNGGA	1 cut(s)	874
PkrI	GCNGC	9 cut(s)	17, 37, 117, 602, 1133, 1360, 1363, 1399, 1649
PleI	GAGTC	2 cut(s)	284, 878
PluTI	GGCGCC	1 cut(s)	539
PpsI	GAGTC	2 cut(s)	284, 878
Ppu21I	YACGTR	1 cut(s)	1869
PpuMI	RGGWCCY	1 cut(s)	1000
Psp5II	RGGWCCY	1 cut(s)	1000
Psp6I	CCWGG	6 cut(s)	590, 742, 874, 1002, 1430, 1440
PspFI	CCCAGC	1 cut(s)	670
PspGI	CCWGG	6 cut(s)	590, 742, 874, 1002, 1430, 1440
PspN4I	GGNNCC	5 cut(s)	202, 537, 829, 1073, 1095
PspPI	GGNCC	2 cut(s)	1000, 1072
PspPPI	RGGWCCY	1 cut(s)	1000
PspXI	VCTCGAGB	1 cut(s)	693
PstI	CTGCAG	1 cut(s)	17
PstNI	CAGNNNCTG	2 cut(s)	1301, 1364
PsuI	RGATCY	1 cut(s)	1699
PsyI	GACNNNGTC	1 cut(s)	1019
PvuII	CAGCTG	2 cut(s)	1109, 1364
RsaI	GTAC	7 cut(s)	365, 624, 794, 1334, 1502, 1715, 1760
RsaNI	GTAC	7 cut(s)	364, 623, 793, 1333, 1501, 1714, 1759
RseI	CAYNNNNRTG	4 cut(s)	343, 384, 573, 1539
SapI	GCTCTTC	1 cut(s)	1760
SaqAI	TTAA	2 cut(s)	134, 818
SatI	GCNGC	9 cut(s)	16, 36, 116, 601, 1132, 1359, 1362, 1398, 1648
Sau3AI	GATC	5 cut(s)	432, 665, 739, 993, 1699
Sau96I	GGNCC	2 cut(s)	1000, 1072
SchI	GAGTC	2 cut(s)	285, 878
ScrFI	CCNGG	8 cut(s)	592, 744, 876, 966, 967, 1004, 1432, 1442
SduI	GDGCHC	3 cut(s)	350, 973, 1098
SfaNI	GCATC	3 cut(s)	311, 976, 1112
SfcI	CTRYAG	2 cut(s)	13, 513
SfoI	GGCGCC	1 cut(s)	537
Sfr274I	CTCGAG	1 cut(s)	693
SinI	GGWCC	2 cut(s)	1000, 1072
SlaI	CTCGAG	1 cut(s)	693
SmaI	CCCGGG	1 cut(s)	967
SmiMI	CAYNNNNRTG	4 cut(s)	343, 384, 573, 1539
SmlI	CTYRAG	2 cut(s)	693, 1804
SmoI	CTYRAG	2 cut(s)	693, 1804
SnaBI	TACGTA	1 cut(s)	1869
SsiI	CCGC	5 cut(s)	36, 474, 600, 647, 1131
SspDI	GGCGCC	1 cut(s)	535
SspMI	CTAG	1 cut(s)	560
StyD4I	CCNGG	8 cut(s)	590, 742, 874, 964, 965, 1002, 1430, 1440
StyI	CCWWGG	1 cut(s)	1382
TaaI	ACNGT	8 cut(s)	148, 232, 238, 586, 727, 1068, 1609, 1681
TaiI	ACGT	3 cut(s)	863, 1179, 1871
TaqI	TCGA	3 cut(s)	633, 664, 694
TatI	WGTACW	4 cut(s)	363, 792, 1332, 1758
TauI	GCSGC	3 cut(s)	38, 603, 1134
TfiI	GAWTC	5 cut(s)	157, 1184, 1338, 1567, 1790
Tru1I	TTAA	2 cut(s)	134, 818
Tru9I	TTAA	2 cut(s)	134, 818
TscAI	CASTG	8 cut(s)	575, 619, 829, 996, 1073, 1306, 1677, 1777
TseFI	GTSAC	2 cut(s)	232, 911
TseI	GCWGC	6 cut(s)	15, 115, 1358, 1361, 1397, 1647
Tsp45I	GTSAC	2 cut(s)	232, 911
TspGWI	ACGGA	2 cut(s)	163, 1629
TspMI	CCCGGG	1 cut(s)	965
TspRI	CASTG	8 cut(s)	575, 619, 829, 996, 1073, 1306, 1677, 1777
Tth111I	GACNNNGTC	1 cut(s)	1019
Van91I	CCANNNNNTGG	3 cut(s)	1254, 1431, 1702
VneI	GTGCAC	1 cut(s)	346
VpaK11BI	GGWCC	2 cut(s)	1000, 1072
XapI	RAATTY	4 cut(s)	61, 293, 772, 1579
XceI	RCATGY	2 cut(s)	706, 1483
XcmI	CCANNNNNNNNNTGG	2 cut(s)	633, 1631
XhoI	CTCGAG	1 cut(s)	693
XmaI	CCCGGG	1 cut(s)	965
XspI	CTAG	1 cut(s)	560
Zsp2I	ATGCAT	1 cut(s)	304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.