Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001351

Physical Location & Seq

Genomic Coordinates Forward (+)

28612560 .. 28616918

Gene Structure 4359 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1142400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGAGCAAAGTTTTCAACTTTTTGCGATTCTGGTGATTCTGGGTTTGACGAACTTGGGCGGAGCTGCCGCTGGCGGCGGTGGTTTTGGGGAACAGCCGCTTTCCAAGATTGCCATAGAGAAAGCCACCCTCAACCTCTCATGCACTGCCTCCATCAATGCCTCTCCTTTGGTGCTCGGCTTGCAGGGTGAGGATACCGAATGGGTGACTGTGAAGCTTGAGAACCATACTCCGACTGAAGATGATTGGGTTGGAGTCTTTTCTCCTGCAAGATTCAATGCATCGATGTGCCTGCCAGTGAATGCACCAAAAGAACAAACTCCCTATATATGCACTGCCCCTATAAAGTACAAGTATGCAACAGACTCCAATGCAAATTATACAAAGACTGGCAAAGCTACTTTGAAGTTTCAGCTTATCAATCAGCGGGCAGATTTCTCGTTCGCATTATTTACAGGCGGGTTATTGAATCCAAAACTGGTGGCAGTTTCAAACTTCATAACCTTTTCCAATCCCAAAGCACCTGTGTATCCACGCCTTGCTCAAGGGAAATCTTGGAACGAAATGACAGTAACTTGGACAAGTGGCTACGACATAAATGAAGCTGTTCCATTTGTTGAGTGGGGTGTAGAGGGGCAAACCCGAAAGCGATCCGGAGCTGGAACATTGACATTTGATCGGAGCATGATGTGTGGTGCACCTGCAAAGACAGTCGGATGGCGTGATCCTGGTTTCATACATACAAGTTTCCTGAAGGATTTGTGGCCGAATATCGAGCAATTCTACAGGATGGGCCATCGTTTATCCAATGGTACAATTATTTGGAGCAAGAAGTACATATTCAGATCATCTCCATATCCTGGACAGGACTCCTTACAGCGTGTCATAATGTTCGGTGACATGGGGAAGGGGGAGCGCGATGGTTCAAATGAGTACAGTAATTACCAGCCTGGTGCCCTGAACACTACCGATCAAATCATCAGGGATTTGAAAAACATTGATATCGTTTTTCATATTGGAGATTTATCGTATGCCAACGGGTACATCTCACAATGGGACCAGTTCACAGCACAGGTGGAGCCCATTGCATCAACTGTGCCATATATGGTTGGCAGTGGTAATCATGAACGTGATTGGCCTGATTCGGGGTCCTTTTACGACAAAGTGGATTCTGGTGGAGAATGTGGTGTGCTGGCTGAGACCATGTTCTATGTTCCAGCTGAGAACCGAGCAAAGTTTTGGTACAAAACCGACTATGGCATGTTTCGCTTCTGCATAGCTGACACTGAACACGACTGGAGAGAAGGATCAGAGCAGTACAAGTTCATCGAGAATTGCCTTGCAACTGTCGATAGACAGAAACAGCCTTGGTTGATCTTTGCCGCTCATCGAGTGCTTGGCTATTCCTCTGACTATTGGTATGGGCAGGAGGGCTCATTTGAAGAGCCTATGGGAAGGGAAAGCTTGCAGAGACTCTGGCAGAAATACAAGGTTGACATTGCCTTTTACGGCCATGTCCATAACTATGAAAGATCATGCCCCGTCTACCAGAGCCAATGCGTAACTTCAGAAAAATCCCATTATTCCGGCGCTGTGAAAGGAACAATCCATGTTGTGGTTGGTGGCGCAGGGAGCCACTTATCTGATTTCAGCCAAGTGACTCCTAACTGGAGTATTTTCAGAGATTATGACTACGGCTTTGTCAAAATGACGGCATTCAATCACACGTCTCTCCTGTTCGAGTACAAGAAAAGCAGCGACGGCAACGTCTACGATTCCTTCACCATATCAAGGGACTACACAGACGTACTGGCTTGTGTGCACGACAGTTGCGAAGCGACGACCGCTGCGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005618 GO:0005623 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030312 GO:0042578 GO:0044237 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

619

Amino Acids

69.29

Weight (kDa)

5.67

Isoelectric Point (pI)

40.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
fn3_PAP	PF17808	52 - 171	4.8e-47	Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N	PF16656	178 - 282	1.1e-08	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	294 - 509	1e-15	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	534 - 593	1.1e-18	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24890 AT5G50400 AT5G50400
fragaria_vesca	FvH4_5g08240 FvH4_5g08241 FvH4_5g08282 FvH4_5g08283 FvH4_5g08284
malus_domestica	MD06G1142200.v1.1 MD06G1142300.v1.1 MD06G1142400.v1.1 MD14G1157600.v1.1
prunus_persica	Prupe.5G151400_v2.0.a1 Prupe.5G151500_v2.0.a1 Prupe.5G151600_v2.0.a1
pyrus_communis	pycom06g13200 pycom06g13210 pycom06g13220
rosa_chinensis	RchiOBHm_Chr7g0190231 RchiOBHm_Chr7g0190241 RchiOBHm_Chr7g0190251 RchiOBHm_Chr7g0190261 RchiOBHm_Chr7g0190281
rosa_laevigata	RLG00000004547 RLG00000004549 RLG00000004550 RLG00000004551
rosa_multiflora	Rmu_sc0004886.1_g000021 Rmu_sc0005487.1_g000006 Rmu_sc0031901.1_g000001 Rmu_sc0031902.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264770
rosa_rugosa	Rorug06G0503100.1 Rorug06G0503300 Rorug06G0503400
rosa_samantha	Rh7AG108500 Rh7AG108800 Rh7AG108900 Rh7AG109100 Rh7BG111100 Rh7BG111300 Rh7BG111400 Rh7BG111500 Rh7CG113500 Rh7CG113700 Rh7CG113800 Rh7CG113900 Rh7DG112100
rosa_wichuraiana	Rw7G009410 Rw7G009420 Rw7G009440 Rw7G009450 Rw7G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	709
AasI	GACNNNNNNGTC	2 cut(s)	1766, 1849
Acc36I	ACCTGC	1 cut(s)	709
AccB1I	GGYRCC	1 cut(s)	953
AccB7I	CCANNNNNTGG	2 cut(s)	860, 1615
AccBSI	CCGCTC	1 cut(s)	1385
AccI	GTMKAC	2 cut(s)	1545, 1770
AccII	CGCG	1 cut(s)	918
AccIII	TCCGGA	1 cut(s)	653
AciI	CCGC	9 cut(s)	60, 69, 75, 78, 98, 427, 459, 1383, 1845
AclWI	GGATC	3 cut(s)	645, 719, 1315
AcoI	YGGCCR	2 cut(s)	764, 1510
AcuI	CTGAAG	3 cut(s)	258, 773, 1551
AfaI	GTAC	9 cut(s)	350, 814, 836, 935, 1043, 1244, 1319, 1745, 1809
AfiI	CCNNNNNNNGG	4 cut(s)	860, 1145, 1615, 1791
AflIII	ACRYGT	1 cut(s)	1725
AgsI	TTSAA	9 cut(s)	17, 277, 406, 469, 492, 927, 991, 1442, 1720
AjiI	CACGTC	1 cut(s)	1728
AjnI	CCWGG	3 cut(s)	727, 859, 949
AluBI	AGCT	9 cut(s)	65, 217, 398, 415, 605, 659, 1220, 1280, 1464
AluI	AGCT	9 cut(s)	65, 217, 398, 415, 605, 659, 1220, 1280, 1464
Alw21I	GWGCWC	3 cut(s)	177, 700, 1824
Alw26I	GTCTC	3 cut(s)	1193, 1465, 1734
Alw44I	GTGCAC	2 cut(s)	696, 1820
AlwI	GGATC	3 cut(s)	645, 719, 1315
Aor13HI	TCCGGA	1 cut(s)	653
AoxI	GGCC	4 cut(s)	764, 793, 1136, 1510
ApaLI	GTGCAC	2 cut(s)	696, 1820
ApeKI	GCWGC	3 cut(s)	65, 1755, 1847
ArsI	GACNNNNNNTTYG	2 cut(s)	1682, 1714
Asp700I	GAANNNNTTC	1 cut(s)	606
AspLEI	GCGC	3 cut(s)	918, 1592, 1628
AspS9I	GGNCC	3 cut(s)	793, 1057, 1149
AsuHPI	GGTGA	5 cut(s)	46, 200, 217, 908, 1774
AvaII	GGWCC	2 cut(s)	1057, 1149
BaeGI	GKGCMC	3 cut(s)	700, 958, 1824
BaeI	ACNNNNGTAYC	2 cut(s)	512, 545
BanI	GGYRCC	1 cut(s)	953
BanII	GRGCYC	2 cut(s)	1083, 1436
Bbv12I	GWGCWC	3 cut(s)	177, 700, 1824
BbvI	GCAGC	3 cut(s)	52, 1767, 1834
BccI	CCATC	5 cut(s)	161, 711, 784, 804, 914
BceAI	ACGGC	4 cut(s)	1525, 1711, 1728, 1777
BcgI	CGANNNNNNTGC	4 cut(s)	274, 308, 420, 454
BciT130I	CCWGG	3 cut(s)	729, 861, 951
BciVI	GTATCC	2 cut(s)	187, 540
BcoDI	GTCTC	3 cut(s)	1193, 1465, 1734
BfaI	CTAG	1 cut(s)	1855
BfmI	CTRYAG	1 cut(s)	784
BfoI	RGCGCY	1 cut(s)	1593
BfuAI	ACCTGC	1 cut(s)	709
BfuI	GTATCC	2 cut(s)	187, 540
BisI	GCNGC	7 cut(s)	66, 69, 76, 98, 1383, 1756, 1848
BlsI	GCNGC	7 cut(s)	67, 70, 77, 99, 1384, 1757, 1849
Bme1390I	CCNGG	3 cut(s)	729, 861, 951
Bme18I	GGWCC	2 cut(s)	1057, 1149
BmgBI	CACGTC	1 cut(s)	1728
BmgT120I	GGNCC	3 cut(s)	793, 1057, 1149
BmiI	GGNNCC	5 cut(s)	955, 1058, 1080, 1150, 1634
BmrFI	CCNGG	3 cut(s)	729, 861, 951
BmsI	GCATC	2 cut(s)	290, 1097
BpmI	CTGGAG	2 cut(s)	1318, 1690
BpuEI	CTTGAG	2 cut(s)	239, 528
Bsa29I	ATCGAT	1 cut(s)	284
BsaI	GGTCTC	1 cut(s)	1193
BsaJI	CCNNGG	1 cut(s)	1367
BsaWI	WCCGGW	1 cut(s)	653
Bsc4I	CCNNNNNNNGG	4 cut(s)	860, 1145, 1615, 1791
Bse1I	ACTGG	7 cut(s)	296, 394, 483, 1060, 1301, 1673, 1815
Bse3DI	GCAATG	2 cut(s)	1083, 1497
BseAI	TCCGGA	1 cut(s)	653
BseBI	CCWGG	3 cut(s)	729, 861, 951
BseCI	ATCGAT	1 cut(s)	284
BseDI	CCNNGG	1 cut(s)	1367
BseGI	GGATG	2 cut(s)	722, 795
BseLI	CCNNNNNNNGG	4 cut(s)	860, 1145, 1615, 1791
BseMI	GCAATG	2 cut(s)	1083, 1497
BseMII	CTCAG	2 cut(s)	1188, 1212
BseNI	ACTGG	7 cut(s)	296, 394, 483, 1060, 1301, 1673, 1815
BseSI	GKGCMC	3 cut(s)	700, 958, 1824
BseXI	GCAGC	3 cut(s)	52, 1767, 1834
Bsh1236I	CGCG	1 cut(s)	918
Bsh1285I	CGRYCG	1 cut(s)	1845
BshFI	GGCC	4 cut(s)	766, 795, 1138, 1512
BshNI	GGYRCC	1 cut(s)	953
BshVI	ATCGAT	1 cut(s)	284
BsiEI	CGRYCG	1 cut(s)	1845
BsiHKAI	GWGCWC	3 cut(s)	177, 700, 1824
BsiSI	CCGG	2 cut(s)	654, 1587
BslFI	GGGAC	2 cut(s)	1070, 1808
BslI	CCNNNNNNNGG	4 cut(s)	860, 1145, 1615, 1791
BsmAI	GTCTC	3 cut(s)	1193, 1465, 1734
BsmBI	CGTCTC	1 cut(s)	1734
BsmFI	GGGAC	2 cut(s)	1070, 1808
BsmI	GAATGC	2 cut(s)	307, 1715
BsnI	GGCC	4 cut(s)	766, 795, 1138, 1512
Bso31I	GGTCTC	1 cut(s)	1193
Bsp1286I	GDGCHC	6 cut(s)	177, 700, 958, 1083, 1436, 1824
Bsp13I	TCCGGA	1 cut(s)	653
Bsp143I	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
BspACI	CCGC	9 cut(s)	60, 69, 75, 78, 98, 427, 459, 1383, 1845
BspANI	GGCC	4 cut(s)	766, 795, 1138, 1512
BspCNI	CTCAG	2 cut(s)	1189, 1213
BspDI	ATCGAT	1 cut(s)	284
BspEI	TCCGGA	1 cut(s)	653
BspFNI	CGCG	1 cut(s)	918
BspHI	TCATGA	1 cut(s)	1123
BspLI	GGNNCC	5 cut(s)	955, 1058, 1080, 1150, 1634
BspMI	ACCTGC	1 cut(s)	709
BspPI	GGATC	3 cut(s)	645, 719, 1315
BspQI	GCTCTTC	1 cut(s)	1437
BspT107I	GGYRCC	1 cut(s)	953
BspTNI	GGTCTC	1 cut(s)	1193
BsrBI	CCGCTC	1 cut(s)	1385
BsrDI	GCAATG	2 cut(s)	1083, 1497
BsrI	ACTGG	7 cut(s)	296, 394, 483, 1060, 1301, 1673, 1815
BssECI	CCNNGG	1 cut(s)	1367
BssMI	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
BssT1I	CCWWGG	1 cut(s)	1367
Bst2UI	CCWGG	3 cut(s)	729, 861, 951
Bst4CI	ACNGT	7 cut(s)	211, 571, 712, 938, 1096, 1348, 1829
Bst6I	CTCTTC	1 cut(s)	1437
BstC8I	GCNNGC	6 cut(s)	73, 182, 293, 429, 1194, 1466
BstDEI	CTNAG	2 cut(s)	1197, 1221
BstF5I	GGATG	2 cut(s)	722, 795
BstFNI	CGCG	1 cut(s)	918
BstH2I	RGCGCY	1 cut(s)	1593
BstHHI	GCGC	3 cut(s)	918, 1592, 1628
BstKTI	GATC	8 cut(s)	653, 679, 727, 848, 973, 1310, 1377, 1535
BstMAI	GTCTC	3 cut(s)	1193, 1465, 1734
BstMBI	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
BstMCI	CGRYCG	1 cut(s)	1845
BstMWI	GCNNNNNNNGC	5 cut(s)	181, 1266, 1632, 1761, 1844
BstNI	CCWGG	3 cut(s)	729, 861, 951
BstNSI	RCATGY	1 cut(s)	1264
BstSCI	CCNGG	3 cut(s)	727, 859, 949
BstSFI	CTRYAG	1 cut(s)	784
BstSLI	GKGCMC	3 cut(s)	700, 958, 1824
BstUI	CGCG	1 cut(s)	918
BstV1I	GCAGC	3 cut(s)	52, 1767, 1834
Bsu15I	ATCGAT	1 cut(s)	284
BsuI	GTATCC	2 cut(s)	187, 540
BsuRI	GGCC	4 cut(s)	766, 795, 1138, 1512
BsuTUI	ATCGAT	1 cut(s)	284
BtgZI	GCGATG	1 cut(s)	933
BtrI	CACGTC	1 cut(s)	1728
BtsCI	GGATG	2 cut(s)	722, 795
BtsI	GCAGTG	3 cut(s)	144, 333, 1120
BtsIMutI	CAGTG	5 cut(s)	144, 303, 333, 1120, 1284
BveI	ACCTGC	1 cut(s)	709
Cac8I	GCNNGC	6 cut(s)	73, 182, 293, 429, 1194, 1466
CciI	TCATGA	1 cut(s)	1123
CfoI	GCGC	3 cut(s)	918, 1592, 1628
Cfr13I	GGNCC	3 cut(s)	793, 1057, 1149
ClaI	ATCGAT	1 cut(s)	284
CseI	GACGC	1 cut(s)	1839
Csp6I	GTAC	9 cut(s)	349, 813, 835, 934, 1042, 1243, 1318, 1744, 1808
CspCI	CAANNNNNGTGG	2 cut(s)	462, 497
CviAII	CATG	9 cut(s)	141, 685, 901, 1124, 1204, 1261, 1514, 1536, 1610
CviQI	GTAC	9 cut(s)	349, 813, 835, 934, 1042, 1243, 1318, 1744, 1808
DdeI	CTNAG	2 cut(s)	1197, 1221
DpnI	GATC	8 cut(s)	652, 678, 726, 847, 972, 1309, 1376, 1534
DpnII	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
DrdI	GACNNNNNNGTC	2 cut(s)	1766, 1849
DseDI	GACNNNNNNGTC	2 cut(s)	1766, 1849
EaeI	YGGCCR	2 cut(s)	764, 1510
Eam1104I	CTCTTC	1 cut(s)	1437
EarI	CTCTTC	1 cut(s)	1437
EciI	GGCGGA	1 cut(s)	75
Eco130I	CCWWGG	1 cut(s)	1367
Eco24I	GRGCYC	2 cut(s)	1083, 1436
Eco31I	GGTCTC	1 cut(s)	1193
Eco32I	GATATC	1 cut(s)	1003
Eco47I	GGWCC	2 cut(s)	1057, 1149
Eco57I	CTGAAG	3 cut(s)	258, 773, 1551
EcoO109I	RGGNCCY	1 cut(s)	1149
EcoRII	CCWGG	3 cut(s)	727, 859, 949
EcoRV	GATATC	1 cut(s)	1003
EcoT14I	CCWWGG	1 cut(s)	1367
EcoT22I	ATGCAT	1 cut(s)	283
EcoT38I	GRGCYC	2 cut(s)	1083, 1436
ErhI	CCWWGG	1 cut(s)	1367
Esp3I	CGTCTC	1 cut(s)	1734
FaeI	CATG	9 cut(s)	144, 688, 904, 1127, 1207, 1264, 1517, 1539, 1613
FaqI	GGGAC	2 cut(s)	1070, 1808
FatI	CATG	9 cut(s)	140, 684, 900, 1123, 1203, 1260, 1513, 1535, 1609
FauI	CCCGC	2 cut(s)	420, 452
FblI	GTMKAC	2 cut(s)	1545, 1770
Fnu4HI	GCNGC	7 cut(s)	66, 69, 76, 98, 1383, 1756, 1848
FokI	GGATG	2 cut(s)	729, 802
FriOI	GRGCYC	2 cut(s)	1083, 1436
Fsp4HI	GCNGC	7 cut(s)	66, 69, 76, 98, 1383, 1756, 1848
FspBI	CTAG	1 cut(s)	1855
GlaI	GCGC	3 cut(s)	917, 1591, 1627
GluI	GCNGC	7 cut(s)	66, 69, 76, 98, 1383, 1756, 1848
GsuI	CTGGAG	2 cut(s)	1318, 1690
HaeII	RGCGCY	1 cut(s)	1593
HaeIII	GGCC	4 cut(s)	766, 795, 1138, 1512
HapII	CCGG	2 cut(s)	654, 1587
HgaI	GACGC	1 cut(s)	1839
HhaI	GCGC	3 cut(s)	918, 1592, 1628
Hin1II	CATG	9 cut(s)	144, 688, 904, 1127, 1207, 1264, 1517, 1539, 1613
Hin6I	GCGC	3 cut(s)	916, 1590, 1626
HinP1I	GCGC	3 cut(s)	916, 1590, 1626
HincII	GTYRAC	1 cut(s)	1495
HindII	GTYRAC	1 cut(s)	1495
HindIII	AAGCTT	2 cut(s)	215, 1462
HpaII	CCGG	2 cut(s)	654, 1587
HphI	GGTGA	5 cut(s)	46, 200, 217, 908, 1774
Hpy166II	GTNNAC	6 cut(s)	698, 1065, 1495, 1546, 1771, 1822
Hpy188I	TCNGA	9 cut(s)	234, 681, 716, 845, 1312, 1411, 1570, 1645, 1682
Hpy188III	TCNNGA	4 cut(s)	654, 751, 1124, 1330
Hpy8I	GTNNAC	6 cut(s)	698, 1065, 1495, 1546, 1771, 1822
Hpy99I	CGWCG	2 cut(s)	1763, 1843
HpyAV	CCTTC	5 cut(s)	748, 901, 1298, 1449, 1789
HpyCH4III	ACNGT	7 cut(s)	211, 571, 712, 938, 1096, 1348, 1829
HpyCH4IV	ACGT	4 cut(s)	1129, 1727, 1767, 1806
HpyF10VI	GCNNNNNNNGC	5 cut(s)	181, 1266, 1632, 1761, 1844
HpyF3I	CTNAG	2 cut(s)	1197, 1221
HpySE526I	ACGT	4 cut(s)	1129, 1727, 1767, 1806
Hsp92II	CATG	9 cut(s)	144, 688, 904, 1127, 1207, 1264, 1517, 1539, 1613
HspAI	GCGC	3 cut(s)	916, 1590, 1626
Kpn2I	TCCGGA	1 cut(s)	653
Kzo9I	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
LguI	GCTCTTC	1 cut(s)	1437
LmnI	GCTCC	8 cut(s)	4, 62, 656, 681, 825, 913, 1078, 1632
Lsp1109I	GCAGC	3 cut(s)	52, 1767, 1834
LweI	GCATC	2 cut(s)	290, 1097
MaeI	CTAG	1 cut(s)	1855
MaeII	ACGT	4 cut(s)	1129, 1727, 1767, 1806
MaeIII	GTNAC	5 cut(s)	205, 571, 896, 1561, 1657
MalI	GATC	8 cut(s)	652, 678, 726, 847, 972, 1309, 1376, 1534
MbiI	CCGCTC	1 cut(s)	1385
MboI	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
MboII	GAAGA	2 cut(s)	251, 1454
MhlI	GDGCHC	6 cut(s)	177, 700, 958, 1083, 1436, 1824
MluCI	AATT	5 cut(s)	376, 779, 816, 940, 1333
MlyI	GAGTC	5 cut(s)	264, 359, 863, 1467, 1654
MmeI	TCCRAC	3 cut(s)	232, 257, 694
MnlI	CCTC	8 cut(s)	140, 146, 160, 172, 184, 625, 1417, 1423
Mph1103I	ATGCAT	1 cut(s)	283
MroI	TCCGGA	1 cut(s)	653
MroXI	GAANNNNTTC	1 cut(s)	606
MslI	CAYNNNNRTG	3 cut(s)	286, 1128, 1524
MspA1I	CMGCKG	4 cut(s)	71, 427, 1220, 1847
MspI	CCGG	2 cut(s)	654, 1587
MspR9I	CCNGG	3 cut(s)	729, 861, 951
Mva1269I	GAATGC	2 cut(s)	307, 1715
MvaI	CCWGG	3 cut(s)	729, 861, 951
MvnI	CGCG	1 cut(s)	918
MwoI	GCNNNNNNNGC	5 cut(s)	181, 1266, 1632, 1761, 1844
NdeII	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
NlaIII	CATG	9 cut(s)	144, 688, 904, 1127, 1207, 1264, 1517, 1539, 1613
NlaIV	GGNNCC	5 cut(s)	955, 1058, 1080, 1150, 1634
NmeAIII	GCCGAG	1 cut(s)	156
NmuCI	GTSAC	3 cut(s)	205, 896, 1657
NsiI	ATGCAT	1 cut(s)	283
NspI	RCATGY	1 cut(s)	1264
PagI	TCATGA	1 cut(s)	1123
PaqCI	CACCTGC	1 cut(s)	709
PciSI	GCTCTTC	1 cut(s)	1437
PcsI	WCGNNNNNNNCGW	2 cut(s)	1830, 1847
PctI	GAATGC	2 cut(s)	307, 1715
PdmI	GAANNNNTTC	1 cut(s)	606
PfeI	GAWTC	7 cut(s)	28, 37, 273, 469, 1142, 1169, 1775
PflMI	CCANNNNNTGG	2 cut(s)	860, 1615
PfoI	TCCNGGA	1 cut(s)	859
PkrI	GCNGC	7 cut(s)	67, 70, 77, 99, 1384, 1757, 1849
PleI	GAGTC	5 cut(s)	263, 359, 863, 1467, 1654
PpsI	GAGTC	5 cut(s)	263, 359, 863, 1467, 1654
PpuMI	RGGWCCY	1 cut(s)	1149
Psp5II	RGGWCCY	1 cut(s)	1149
Psp6I	CCWGG	3 cut(s)	727, 859, 949
PspGI	CCWGG	3 cut(s)	727, 859, 949
PspN4I	GGNNCC	5 cut(s)	955, 1058, 1080, 1150, 1634
PspPI	GGNCC	3 cut(s)	793, 1057, 1149
PspPPI	RGGWCCY	1 cut(s)	1149
PvuII	CAGCTG	1 cut(s)	1220
RsaI	GTAC	9 cut(s)	350, 814, 836, 935, 1043, 1244, 1319, 1745, 1809
RsaNI	GTAC	9 cut(s)	349, 813, 835, 934, 1042, 1243, 1318, 1744, 1808
RseI	CAYNNNNRTG	3 cut(s)	286, 1128, 1524
SapI	GCTCTTC	1 cut(s)	1437
SatI	GCNGC	7 cut(s)	66, 69, 76, 98, 1383, 1756, 1848
Sau3AI	GATC	8 cut(s)	650, 676, 724, 845, 970, 1307, 1374, 1532
Sau96I	GGNCC	3 cut(s)	793, 1057, 1149
SchI	GAGTC	5 cut(s)	264, 359, 863, 1467, 1654
ScrFI	CCNGG	3 cut(s)	729, 861, 951
SduI	GDGCHC	6 cut(s)	177, 700, 958, 1083, 1436, 1824
SfaNI	GCATC	2 cut(s)	290, 1097
SfcI	CTRYAG	1 cut(s)	784
SinI	GGWCC	2 cut(s)	1057, 1149
SmiMI	CAYNNNNRTG	3 cut(s)	286, 1128, 1524
SmlI	CTYRAG	2 cut(s)	218, 543
SmoI	CTYRAG	2 cut(s)	218, 543
Sse9I	AATT	5 cut(s)	376, 779, 816, 940, 1333
SsiI	CCGC	9 cut(s)	60, 69, 75, 78, 98, 427, 459, 1383, 1845
SspMI	CTAG	1 cut(s)	1855
StyD4I	CCNGG	3 cut(s)	727, 859, 949
StyI	CCWWGG	1 cut(s)	1367
TaaI	ACNGT	7 cut(s)	211, 571, 712, 938, 1096, 1348, 1829
TaiI	ACGT	4 cut(s)	1132, 1730, 1770, 1809
TaqI	TCGA	6 cut(s)	284, 774, 1329, 1350, 1390, 1740
TasI	AATT	5 cut(s)	376, 779, 816, 940, 1333
TatI	WGTACW	5 cut(s)	348, 834, 933, 1317, 1743
TauI	GCSGC	4 cut(s)	71, 78, 100, 1385
TfiI	GAWTC	7 cut(s)	28, 37, 273, 469, 1142, 1169, 1775
TscAI	CASTG	5 cut(s)	151, 303, 340, 1120, 1291
TseFI	GTSAC	3 cut(s)	205, 896, 1657
TseI	GCWGC	3 cut(s)	65, 1755, 1847
Tsp45I	GTSAC	3 cut(s)	205, 896, 1657
TspDTI	ATGAA	7 cut(s)	487, 615, 724, 1001, 1140, 1315, 1542
TspRI	CASTG	5 cut(s)	151, 303, 340, 1120, 1291
Van91I	CCANNNNNTGG	2 cut(s)	860, 1615
VneI	GTGCAC	2 cut(s)	696, 1820
VpaK11BI	GGWCC	2 cut(s)	1057, 1149
XceI	RCATGY	1 cut(s)	1264
XcmI	CCANNNNNNNNNTGG	2 cut(s)	618, 1616
XmiI	GTMKAC	2 cut(s)	1545, 1770
XmnI	GAANNNNTTC	1 cut(s)	606
XspI	CTAG	1 cut(s)	1855
Zsp2I	ATGCAT	1 cut(s)	283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.