RchiOBHm_Chr7g0190281

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001351
Physical Location & Seq
Forward (+)
9422187 .. 9426178
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16993

Sequence Viewer

Length: 1851 bp
ATGGAGCAAATATTTCGACTTTTCACAATTCTGGTGATTTTGGGCTCCACCCATTTGGGCTCAGATTGTGCTCAAATCTATGGAGACCAGCCACTCGCCAAGATTAACATTCGGAAAACCACTCTCGCCATTCATGAGTCGGCCTCCATTAGAGCCTACCCTTCTGTTCTTGGCATACAGGGTGAGGATACAGATTGGGTGACTGTGGATCTCAAGTACCCTGATGCTTCTGATAATGACTGGGTTGGAGTTTTTTCTCCTGGAAGACTCAATGCATCACAGTGCCCACCAGTAGATGGTCAAAGAGAACAAACTCCATATATATGCACTGCTCCTATAAAGTACAAATATGCAAAACAAGACAATCCAGGTTATACAAAGACCGGCAATGCAACTTTGAAGTTTCAGCTTATAAATCAGCGAGCAGATTTCTCTTTTGCATTATTTTCAGGCGGATTGTCAAATCCAAAATTGGTGGCAATTTCAAATTTCTTGACATTTGCCAATCCTAAAGCGCCTGTTTATCCACGTCTGGCTCAAGGGAAGTCTTGGAATGAAATGACAGTAACTTGGACTAGTGGCTACGGCGTAGGCGAAGCTGTTCCATTTGTTGAGTGGGGTATGAAGGGAGATATTCGAAAGCGATCTGCAGCTGCAACATTGACATTTGATCAGAACAGCTTGTGTGGTTCACCTGCAAGGACAGTTGGGTGGCGCGATCCAGGTTTCATACACACAAGTTTCCTGAACAACTTGTGGCCAAACACATTGTACACCTATAGGATGGGTCATCGTTACTTGGATGGTCAGTATATTTGGAGCAAGGTCTACTCATTCAAATCATCTCCATATCCTGGACAAGATTCCTTGCAGCGCGTTGTAATATTTGGTGACATGGGAAAGGCAGAGCGTGATGGTTCAAATGAGTACAGTGATTATCAACCTGGTGCTCTGAACACTACAGATCAAGTCATCAGTGACTTGAACAACATTGACATAGTTTTTCATATAGGAGATCTATCATATGCAAATGGCTATATTTCACAATGGGACCAATTCACAGCACAGGTGGAGCCCATTGCATCAACTGTTCCATATATGGTTGGCAGTGGTAATCATGAACGTGATTGGCCTGATTCGGGGTCCTATTACAACGGAATGGATTCAGGTGGAGAATGTGGTGTGCTTGGTGAGACCATGTTTTACGTTCCAGCTCACAACCGAGCTAAATATTGGTACTCTACAGATTATGGATTGTTTCACTTCTGTATAGCTGACACAGAACATGACTGGAGGGAGGGAACAGAGCAGTACAAGTTCATTGAGAATTGCCTTGCATCCGTGGATAGACAAAAGCAACCTTGGTTGATCTTTGCAGGTCATCGTGTCCTTGGTTATACATCTGACAAATGGTATAGTCAAGGGGGCGCATTTGAAGAGCCTATGGGAAGGGAAAGCTTGCAGAAACTTTGGCAGAAGTACAAGGTAGACATCGCATTTTATGGCCATGTCCATAACTATGAAAGGACATGCCCCATCTATGAGAACCAATGCGTATCTTCAGAAAGATCTCACTACTCTGGGAAATTTAATGGAACAATCCATGTTGTTGTTGGAGGAGCCGGAAGCCACTTATCTGACTTCAGCCAACAACAACCAGGTTGGAGTCTCTACAGAGATCATGACTGGGGATTTGTAAAATTGACTGCATTCAATCACACATCCCTCCTCTTTGAGTACAAGAAAAGCAGCGACGGGAAGGTTTACGATTCCTTCACCATATCAAGGGACTACACAGATGTATTGGCTTGTGTTCACGACAGTTGCGAACCAACGACAGCAGCATTTTAG

Protein Analysis

616

Amino Acids

69.44

Weight (kDa)

5.77

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 50 - 169 8.1e-45 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 176 - 280 2e-13 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 292 - 507 3.8e-16 Calcineurin-like phosphoesterase
Metallophos_C PF14008 532 - 591 1.2e-18 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AarI CACCTGC 1 cut(s) 703
Acc36I ACCTGC 2 cut(s) 703, 1367
AccB7I CCANNNNNTGG 2 cut(s) 296, 854
AccI GTMKAC 2 cut(s) 828, 1488
AccII CGCG 2 cut(s) 717, 876
AciI CCGC 1 cut(s) 453
AclWI GGATC 2 cut(s) 216, 713
AcoI YGGCCR 2 cut(s) 758, 1504
AcsI RAATTY 2 cut(s) 487, 1586
AcuI CTGAAG 2 cut(s) 1545, 1627
AfaI GTAC 8 cut(s) 218, 344, 773, 929, 1238, 1313, 1481, 1739
AfiI CCNNNNNNNGG 4 cut(s) 296, 854, 1139, 1785
AgsI TTSAA 7 cut(s) 400, 486, 838, 921, 985, 1436, 1714
AhlI ACTAGT 1 cut(s) 575
AjiI CACGTC 1 cut(s) 530
AjnI CCWGG 6 cut(s) 259, 367, 721, 853, 943, 1657
AluBI AGCT 8 cut(s) 409, 599, 653, 681, 1214, 1226, 1274, 1458
AluI AGCT 8 cut(s) 409, 599, 653, 681, 1214, 1226, 1274, 1458
Alw21I GWGCWC 2 cut(s) 73, 952
Alw26I GTCTC 3 cut(s) 78, 1187, 1673
AlwI GGATC 2 cut(s) 216, 713
AoxI GGCC 4 cut(s) 141, 758, 1130, 1504
ApeKI GCWGC 5 cut(s) 650, 653, 871, 1749, 1841
ApoI RAATTY 2 cut(s) 487, 1586
ArsI GACNNNNNNTTYG 2 cut(s) 1676, 1708
Asp700I GAANNNNTTC 2 cut(s) 600, 1162
AspLEI GCGC 4 cut(s) 517, 717, 876, 1430
AspS9I GGNCC 2 cut(s) 1051, 1143
AsuHPI GGTGA 7 cut(s) 46, 194, 211, 684, 902, 1202, 1768
AsuII TTCGAA 1 cut(s) 637
AvaII GGWCC 2 cut(s) 1051, 1143
BaeGI GKGCMC 1 cut(s) 287
BalI TGGCCA 2 cut(s) 760, 1506
BanII GRGCYC 3 cut(s) 47, 62, 1077
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 2 cut(s) 73, 952
BbvI GCAGC 4 cut(s) 640, 662, 883, 1761
BccI CCATC 5 cut(s) 290, 778, 797, 908, 1544
BceAI ACGGC 1 cut(s) 601
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 6 cut(s) 261, 369, 723, 855, 945, 1659
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 670
BcoDI GTCTC 3 cut(s) 78, 1187, 1673
BcuI ACTAGT 1 cut(s) 575
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 5 cut(s) 648, 778, 960, 1242, 1672
BfoI RGCGCY 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 703, 1367
BfuI GTATCC 1 cut(s) 181
BglII AGATCT 2 cut(s) 1015, 1568
BisI GCNGC 5 cut(s) 651, 654, 872, 1750, 1842
BlsI GCNGC 5 cut(s) 652, 655, 873, 1751, 1843
Bme1390I CCNGG 6 cut(s) 261, 369, 723, 855, 945, 1659
Bme18I GGWCC 2 cut(s) 1051, 1143
BmgBI CACGTC 1 cut(s) 530
BmgT120I GGNCC 2 cut(s) 1051, 1143
BmiI GGNNCC 5 cut(s) 46, 1052, 1074, 1144, 1621
BmrFI CCNGG 6 cut(s) 261, 369, 723, 855, 945, 1659
BmrI ACTGGG 2 cut(s) 250, 1696
BmsI GCATC 4 cut(s) 214, 284, 1091, 1346
BmuI ACTGGG 2 cut(s) 250, 1696
BpiI GAAGAC 1 cut(s) 271
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 1312
Bpu14I TTCGAA 1 cut(s) 637
BpuEI CTTGAG 2 cut(s) 197, 522
BsaI GGTCTC 2 cut(s) 78, 1187
BsaJI CCNNGG 3 cut(s) 1341, 1361, 1390
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 854, 1139, 1785
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 4 cut(s) 245, 290, 1295, 1691
Bse3DI GCAATG 2 cut(s) 394, 1077
BseBI CCWGG 6 cut(s) 261, 369, 723, 855, 945, 1659
BseDI CCNNGG 3 cut(s) 1341, 1361, 1390
BseGI GGATG 4 cut(s) 789, 808, 1337, 1721
BseLI CCNNNNNNNGG 4 cut(s) 296, 854, 1139, 1785
BseMI GCAATG 2 cut(s) 394, 1077
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 4 cut(s) 245, 290, 1295, 1691
BseRI GAGGAG 2 cut(s) 1632, 1718
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 4 cut(s) 640, 662, 883, 1761
Bsh1236I CGCG 2 cut(s) 717, 876
BshFI GGCC 4 cut(s) 143, 760, 1132, 1506
BsiHKAI GWGCWC 2 cut(s) 73, 952
BsiSI CCGG 2 cut(s) 384, 1623
BslFI GGGAC 2 cut(s) 1064, 1802
BslI CCNNNNNNNGG 4 cut(s) 296, 854, 1139, 1785
BsmAI GTCTC 3 cut(s) 78, 1187, 1673
BsmFI GGGAC 2 cut(s) 1064, 1802
BsmI GAATGC 1 cut(s) 1709
BsnI GGCC 4 cut(s) 143, 760, 1132, 1506
Bso31I GGTCTC 2 cut(s) 78, 1187
Bsp119I TTCGAA 1 cut(s) 637
Bsp1286I GDGCHC 6 cut(s) 47, 62, 73, 287, 952, 1077
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
BspACI CCGC 1 cut(s) 453
BspANI GGCC 4 cut(s) 143, 760, 1132, 1506
BspCNI CTCAG 1 cut(s) 74
BspFNI CGCG 2 cut(s) 717, 876
BspHI TCATGA 3 cut(s) 133, 1117, 1681
BspLI GGNNCC 5 cut(s) 46, 1052, 1074, 1144, 1621
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 703, 1367
BspPI GGATC 2 cut(s) 216, 713
BspQI GCTCTTC 1 cut(s) 1431
BspT104I TTCGAA 1 cut(s) 637
BspTNI GGTCTC 2 cut(s) 78, 1187
BsrDI GCAATG 2 cut(s) 394, 1077
BsrFI RCCGGY 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 4 cut(s) 245, 290, 1295, 1691
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 3 cut(s) 1341, 1361, 1390
BssMI GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
BssT1I CCWWGG 2 cut(s) 1361, 1390
Bst2UI CCWGG 6 cut(s) 261, 369, 723, 855, 945, 1659
Bst4CI ACNGT 7 cut(s) 205, 282, 565, 706, 932, 1090, 1823
Bst6I CTCTTC 1 cut(s) 1431
BstAUI TGTACA 1 cut(s) 771
BstBI TTCGAA 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 423, 1460
BstDEI CTNAG 1 cut(s) 61
BstDSI CCRYGG 1 cut(s) 1341
BstF5I GGATG 4 cut(s) 789, 808, 1337, 1721
BstFNI CGCG 2 cut(s) 717, 876
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 4 cut(s) 517, 717, 876, 1430
BstKTI GATC 9 cut(s) 211, 647, 673, 721, 967, 1018, 1371, 1571, 1681
BstMAI GTCTC 3 cut(s) 78, 1187, 1673
BstMBI GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
BstNI CCWGG 6 cut(s) 261, 369, 723, 855, 945, 1659
BstNSI RCATGY 1 cut(s) 1533
BstSCI CCNGG 6 cut(s) 259, 367, 721, 853, 943, 1657
BstSFI CTRYAG 5 cut(s) 648, 778, 960, 1242, 1672
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 2 cut(s) 717, 876
BstV1I GCAGC 4 cut(s) 640, 662, 883, 1761
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 3 cut(s) 208, 1015, 1568
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 3 cut(s) 208, 1015, 1568
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 4 cut(s) 143, 760, 1132, 1506
BtgI CCRYGG 1 cut(s) 1341
BtgZI GCGATG 1 cut(s) 1477
BtrI CACGTC 1 cut(s) 530
BtsCI GGATG 4 cut(s) 789, 808, 1337, 1721
BtsI GCAGTG 2 cut(s) 327, 1114
BtsIMutI CAGTG 5 cut(s) 287, 327, 937, 982, 1114
BveI ACCTGC 2 cut(s) 703, 1367
Cac8I GCNNGC 2 cut(s) 423, 1460
CciI TCATGA 3 cut(s) 133, 1117, 1681
CfoI GCGC 4 cut(s) 517, 717, 876, 1430
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 1051, 1143
CsiI ACCWGGT 2 cut(s) 943, 1657
Csp6I GTAC 8 cut(s) 217, 343, 772, 928, 1237, 1312, 1480, 1738
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 9 cut(s) 134, 895, 1118, 1198, 1286, 1508, 1530, 1604, 1682
CviQI GTAC 8 cut(s) 217, 343, 772, 928, 1237, 1312, 1480, 1738
DdeI CTNAG 1 cut(s) 61
DpnI GATC 9 cut(s) 210, 646, 672, 720, 966, 1017, 1370, 1570, 1680
DpnII GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
EaeI YGGCCR 2 cut(s) 758, 1504
Eam1104I CTCTTC 1 cut(s) 1431
EarI CTCTTC 1 cut(s) 1431
EciI GGCGGA 1 cut(s) 468
Eco130I CCWWGG 2 cut(s) 1361, 1390
Eco24I GRGCYC 3 cut(s) 47, 62, 1077
Eco31I GGTCTC 2 cut(s) 78, 1187
Eco47I GGWCC 2 cut(s) 1051, 1143
Eco57I CTGAAG 2 cut(s) 1545, 1627
EcoO109I RGGNCCY 1 cut(s) 1143
EcoRII CCWGG 6 cut(s) 259, 367, 721, 853, 943, 1657
EcoT14I CCWWGG 2 cut(s) 1361, 1390
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 3 cut(s) 47, 62, 1077
ErhI CCWWGG 2 cut(s) 1361, 1390
FaeI CATG 9 cut(s) 137, 898, 1121, 1201, 1289, 1511, 1533, 1607, 1685
FaqI GGGAC 2 cut(s) 1064, 1802
FatI CATG 9 cut(s) 133, 894, 1117, 1197, 1285, 1507, 1529, 1603, 1681
FauNDI CATATG 1 cut(s) 1024
FbaI TGATCA 1 cut(s) 670
FblI GTMKAC 2 cut(s) 828, 1488
Fnu4HI GCNGC 5 cut(s) 651, 654, 872, 1750, 1842
FokI GGATG 4 cut(s) 796, 815, 1324, 1708
FriOI GRGCYC 3 cut(s) 47, 62, 1077
Fsp4HI GCNGC 5 cut(s) 651, 654, 872, 1750, 1842
FspBI CTAG 1 cut(s) 576
GlaI GCGC 4 cut(s) 516, 716, 875, 1429
GluI GCNGC 5 cut(s) 651, 654, 872, 1750, 1842
GsuI CTGGAG 1 cut(s) 1312
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 4 cut(s) 143, 760, 1132, 1506
HapII CCGG 2 cut(s) 384, 1623
HhaI GCGC 4 cut(s) 517, 717, 876, 1430
Hin1II CATG 9 cut(s) 137, 898, 1121, 1201, 1289, 1511, 1533, 1607, 1685
Hin6I GCGC 4 cut(s) 515, 715, 874, 1428
HinP1I GCGC 4 cut(s) 515, 715, 874, 1428
HindIII AAGCTT 1 cut(s) 1456
HinfI GANTC 7 cut(s) 137, 267, 863, 1136, 1163, 1666, 1769
HpaII CCGG 2 cut(s) 384, 1623
HphI GGTGA 7 cut(s) 46, 194, 211, 684, 902, 1202, 1768
Hpy166II GTNNAC 6 cut(s) 692, 774, 829, 1489, 1765, 1816
Hpy188I TCNGA 8 cut(s) 64, 114, 232, 675, 954, 1405, 1564, 1639
Hpy188III TCNNGA 6 cut(s) 134, 493, 745, 1118, 1682, 1817
Hpy8I GTNNAC 6 cut(s) 692, 774, 829, 1489, 1765, 1816
Hpy99I CGWCG 1 cut(s) 1757
HpyAV CCTTC 5 cut(s) 171, 619, 1443, 1753, 1783
HpyCH4III ACNGT 7 cut(s) 205, 282, 565, 706, 932, 1090, 1823
HpyCH4IV ACGT 3 cut(s) 529, 1123, 1206
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 3 cut(s) 529, 1123, 1206
Hsp92II CATG 9 cut(s) 137, 898, 1121, 1201, 1289, 1511, 1533, 1607, 1685
HspAI GCGC 4 cut(s) 515, 715, 874, 1428
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
LguI GCTCTTC 1 cut(s) 1431
LmnI GCTCC 6 cut(s) 4, 50, 337, 819, 1072, 1619
Lsp1109I GCAGC 4 cut(s) 640, 662, 883, 1761
LweI GCATC 4 cut(s) 214, 284, 1091, 1346
MabI ACCWGGT 2 cut(s) 943, 1657
MaeI CTAG 1 cut(s) 576
MaeII ACGT 3 cut(s) 529, 1123, 1206
MaeIII GTNAC 5 cut(s) 199, 565, 794, 890, 977
MalI GATC 9 cut(s) 210, 646, 672, 720, 966, 1017, 1370, 1570, 1680
MboI GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
MboII GAAGA 3 cut(s) 276, 1448, 1551
MflI RGATCY 3 cut(s) 208, 1015, 1568
MhlI GDGCHC 6 cut(s) 47, 62, 73, 287, 952, 1077
MlsI TGGCCA 2 cut(s) 760, 1506
MluCI AATT 8 cut(s) 27, 470, 480, 487, 1055, 1327, 1586, 1700
MluNI TGGCCA 2 cut(s) 760, 1506
MlyI GAGTC 3 cut(s) 146, 261, 1675
MmeI TCCRAC 3 cut(s) 226, 1594, 1643
MnlI CCTC 7 cut(s) 154, 178, 1287, 1291, 1610, 1736, 1739
Mox20I TGGCCA 2 cut(s) 760, 1506
Mph1103I ATGCAT 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 600, 1162
MscI TGGCCA 2 cut(s) 760, 1506
MseI TTAA 2 cut(s) 105, 1590
MslI CAYNNNNRTG 4 cut(s) 280, 322, 1122, 1518
Msp20I TGGCCA 2 cut(s) 760, 1506
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 384, 1623
MspR9I CCNGG 6 cut(s) 261, 369, 723, 855, 945, 1659
Mva1269I GAATGC 1 cut(s) 1709
MvaI CCWGG 6 cut(s) 261, 369, 723, 855, 945, 1659
MvnI CGCG 2 cut(s) 717, 876
NdeI CATATG 1 cut(s) 1024
NdeII GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
NlaIII CATG 9 cut(s) 137, 898, 1121, 1201, 1289, 1511, 1533, 1607, 1685
NlaIV GGNNCC 5 cut(s) 46, 1052, 1074, 1144, 1621
NmuCI GTSAC 3 cut(s) 199, 890, 977
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 1 cut(s) 1533
NspV TTCGAA 1 cut(s) 637
PagI TCATGA 3 cut(s) 133, 1117, 1681
PaqCI CACCTGC 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 1431
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 1824
PctI GAATGC 1 cut(s) 1709
PdmI GAANNNNTTC 2 cut(s) 600, 1162
PfeI GAWTC 4 cut(s) 863, 1136, 1163, 1769
PflMI CCANNNNNTGG 2 cut(s) 296, 854
PfoI TCCNGGA 2 cut(s) 259, 853
PkrI GCNGC 5 cut(s) 652, 655, 873, 1751, 1843
PleI GAGTC 3 cut(s) 145, 261, 1674
PpsI GAGTC 3 cut(s) 145, 261, 1674
PpuMI RGGWCCY 1 cut(s) 1143
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 1143
Psp6I CCWGG 6 cut(s) 259, 367, 721, 853, 943, 1657
PspGI CCWGG 6 cut(s) 259, 367, 721, 853, 943, 1657
PspN4I GGNNCC 5 cut(s) 46, 1052, 1074, 1144, 1621
PspPI GGNCC 2 cut(s) 1051, 1143
PspPPI RGGWCCY 1 cut(s) 1143
PstI CTGCAG 1 cut(s) 652
PsuI RGATCY 3 cut(s) 208, 1015, 1568
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 8 cut(s) 218, 344, 773, 929, 1238, 1313, 1481, 1739
RsaNI GTAC 8 cut(s) 217, 343, 772, 928, 1237, 1312, 1480, 1738
RseI CAYNNNNRTG 4 cut(s) 280, 322, 1122, 1518
SapI GCTCTTC 1 cut(s) 1431
SaqAI TTAA 2 cut(s) 105, 1590
SatI GCNGC 5 cut(s) 651, 654, 872, 1750, 1842
Sau3AI GATC 9 cut(s) 208, 644, 670, 718, 964, 1015, 1368, 1568, 1678
Sau96I GGNCC 2 cut(s) 1051, 1143
SchI GAGTC 3 cut(s) 146, 261, 1675
ScrFI CCNGG 6 cut(s) 261, 369, 723, 855, 945, 1659
SduI GDGCHC 6 cut(s) 47, 62, 73, 287, 952, 1077
SexAI ACCWGGT 2 cut(s) 943, 1657
SfaNI GCATC 4 cut(s) 214, 284, 1091, 1346
SfcI CTRYAG 5 cut(s) 648, 778, 960, 1242, 1672
SfuI TTCGAA 1 cut(s) 637
SinI GGWCC 2 cut(s) 1051, 1143
SmiMI CAYNNNNRTG 4 cut(s) 280, 322, 1122, 1518
SmlI CTYRAG 2 cut(s) 212, 537
SmoI CTYRAG 2 cut(s) 212, 537
SpeI ACTAGT 1 cut(s) 575
Sse9I AATT 8 cut(s) 27, 470, 480, 487, 1055, 1327, 1586, 1700
SsiI CCGC 1 cut(s) 453
SspI AATATT 3 cut(s) 12, 885, 1232
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 6 cut(s) 259, 367, 721, 853, 943, 1657
StyI CCWWGG 2 cut(s) 1361, 1390
TaaI ACNGT 7 cut(s) 205, 282, 565, 706, 932, 1090, 1823
TaiI ACGT 3 cut(s) 532, 1126, 1209
TaqI TCGA 2 cut(s) 16, 637
TasI AATT 8 cut(s) 27, 470, 480, 487, 1055, 1327, 1586, 1700
TatI WGTACW 6 cut(s) 342, 771, 927, 1311, 1479, 1737
TfiI GAWTC 4 cut(s) 863, 1136, 1163, 1769
Tru1I TTAA 2 cut(s) 105, 1590
Tru9I TTAA 2 cut(s) 105, 1590
TscAI CASTG 5 cut(s) 287, 334, 937, 982, 1114
TseFI GTSAC 3 cut(s) 199, 890, 977
TseI GCWGC 5 cut(s) 650, 653, 871, 1749, 1841
Tsp45I GTSAC 3 cut(s) 199, 890, 977
TspDTI ATGAA 8 cut(s) 122, 570, 638, 718, 995, 1134, 1309, 1536
TspGWI ACGGA 2 cut(s) 1170, 1330
TspRI CASTG 5 cut(s) 287, 334, 937, 982, 1114
Van91I CCANNNNNTGG 2 cut(s) 296, 854
VpaK11BI GGWCC 2 cut(s) 1051, 1143
XapI RAATTY 2 cut(s) 487, 1586
XceI RCATGY 1 cut(s) 1533
XcmI CCANNNNNNNNNTGG 2 cut(s) 612, 1610
XmiI GTMKAC 2 cut(s) 828, 1488
XmnI GAANNNNTTC 2 cut(s) 600, 1162
XspI CTAG 1 cut(s) 576
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.