FvH4_5g08284

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001351
Physical Location & Seq
Reverse (-)
4822459 .. 4825666
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08284.t1

Sequence Viewer

Length: 1866 bp
ATGGAGGGCTCAAGTGTCTTGTCTCTGAAAGTTGCTCTGGTACTGGTTTTCTTGTTGCATTACACAGCAGCTTATAGTCACAATAAGCATGGAGGTGATGGGGTACAGCCACTTTCTAAGATTCAAATCCACAGAGCTGTTTACGTGCTTCATGAAAATGCTTCCCTCAAAGCATACCCTGCGCTTCTTGGTATGAAGGGGGAAGACTCTGAGTGGGTAACTGTGAAAGTTGATAGTCCTAATCCTTCTGGAGATGATTGGTTGGCAGTTTTTTCGCCTGCAAATTTCAACTCATCGACTTGTCCATCGACCGACTCTAGACAACAGGCTCCATACATATGCTCAGCTCCGATAAAGTACATGTTTGCCAATGATTCCAGTGCTGATTATACAAAGACTGGCAAAGGATCCTTCAAACTTCTATTGATCAATCAAAGGGAAGACTTTTCATTTGCGTTGTTCTCAGGAGGGCTATCAAATCCAAAATTGGTGGCAGTTTCTAGTGCTATAAGTTTTGTGAATCCGAAGGCACCCCTCTACCCTCGCCTTGCTCAAGGAAAGCAATGGGATGAAATGACAGTAACCTGGACAAGTGGCTATAACATTGATGAAGCTGTACCTTTTGTTGAATGGGGTATTAAGGGAGAGGCTCAAACTCGATCTCCAGCAGGAACATTGACTTTTACACGAGAGAGCATGTGCGGTCCACCAGCACGAACAGTTGGTTGGCGAGATCCTGGTTTCATCCACACTAGTTTCTTAAAAGGCCTTTGGCCAAATTCATTGTACACTTACAATCTCGGTCATAGGTCATCTAATGGTTCCTATATCTGGAGCAAGTCTTACACTTTCAAGTCATCACCATATCCTGGACAGGACTCATTACAGCGTGTAGTTATATTTGGAGACATGGGAAAGGCTGAGCGTGATGGTTCAAATGAATACAGTAATTATCAGCCGGGTTCACTCAATACCACTGATCAACTGATCAAGGACTTAGACAACATTGACATAGTTTTCCACATAGGAGACATTTCTTATGCCAATGGATACATCTCACAGTGGGACCAGTTCACATCACAGGTCGAGCCCATTGCATCAACTGTACCATATATGATTGCAAGTGGCAATCATGAACGAGATACGCCAGGGACCGGATCCTTCTATAATGGTAATGATGCAGGTGGTGAATGTGGTGTGCTGGCTGAGACTATGTTTTATGTTCCTGCTGAGAACCGAGCTAAGTTCTGGTACTCAACAGATTATGGCATGTTCCACTTCTGTGTTGCTGACACTGAACATGACTGGAGGGAAGGAACTGAGCAGTACAAATTTATTGAGAAATGCCTAGCATCAGTTGACAGGCAGAAACAGCCTTGGCTGATATTTGTTGCTCATCGTGTTCTTGGTTATTCCTCTGACTTATATTATGGGTTGGATGGTTCATTTGAAGAGCCAATGGGTAGGGAAAGCATGCAAAAGCTATGGCAAAAGTACAAGGTGGATATTGCTTTCTATGGCCATGTTCATAATTATGAAAGAATATGCCCCATTTACCAGAATCAATGTGTAAACACAGAAAAATCCCATTATTCTGGGACTGTGAACGGAACGATCCACATTGTTGTTGGAGGAGGAGGCAGTCACTTATCAGAATTTAGCCCGGTGCAGACCATTTGGAGCCTTCACAAAGATCATGACTTTGGATTTGTAAAACTCACAGCATTTAACCACTCATCACTTCTCATGGAGTACAAGAAGAGCAGTGATGGTAACGTCTATGATTCCTTCACTATCTCAAGGGACTACAGGGACGTCTTGGCATGCGTGCATGACGGTTGTGAGCCAACTACCTTAGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

622

Amino Acids

69.35

Weight (kDa)

5.77

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 57 - 174 1.6e-45 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 181 - 285 1.5e-12 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 297 - 512 1.2e-17 Calcineurin-like phosphoesterase
Metallophos_C PF14008 537 - 596 3.6e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1172
AatII GACGTC 1 cut(s) 1818
Acc36I ACCTGC 1 cut(s) 1172
AccB1I GGYRCC 1 cut(s) 529
AccB7I CCANNNNNTGG 1 cut(s) 869
AciI CCGC 1 cut(s) 702
AclWI GGATC 6 cut(s) 402, 415, 728, 1152, 1165, 1609
AcoI YGGCCR 2 cut(s) 773, 1519
AcsI RAATTY 4 cut(s) 283, 778, 1331, 1655
AcyI GRCGYC 1 cut(s) 1815
AfiI CCNNNNNNNGG 3 cut(s) 831, 869, 1154
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 7 cut(s) 125, 289, 415, 629, 853, 936, 1451
AhlI ACTAGT 1 cut(s) 752
AjnI CCWGG 4 cut(s) 584, 736, 868, 1147
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
AleI CACNNNNGTG 1 cut(s) 1281
AluBI AGCT 7 cut(s) 71, 137, 347, 614, 1241, 1483, 1859
AluI AGCT 7 cut(s) 71, 137, 347, 614, 1241, 1483, 1859
Alw26I GTCTC 4 cut(s) 27, 900, 1023, 1202
AlwI GGATC 6 cut(s) 402, 415, 728, 1152, 1165, 1609
AoxI GGCC 3 cut(s) 766, 773, 1519
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 4 cut(s) 283, 778, 1331, 1655
ArsI GACNNNNNNTTYG 4 cut(s) 434, 466, 1691, 1723
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 3 cut(s) 704, 1066, 1152
AsuC2I CCSGG 2 cut(s) 960, 1664
AsuHPI GGTGA 3 cut(s) 107, 852, 1199
AvaII GGWCC 3 cut(s) 704, 1066, 1152
BalI TGGCCA 2 cut(s) 775, 1521
BamHI GGATCC 2 cut(s) 407, 1157
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 2 cut(s) 11, 1092
BauI CACGAG 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 210, 447
BbvI GCAGC 1 cut(s) 80
BccI CCATC 5 cut(s) 92, 313, 923, 1433, 1763
BcgI CGANNNNNNTGC 4 cut(s) 255, 289, 1076, 1110
BciT130I CCWGG 4 cut(s) 586, 738, 870, 1149
BciVI GTATCC 1 cut(s) 1043
BclI TGATCA 3 cut(s) 426, 979, 987
BcnI CCSGG 2 cut(s) 960, 1664
BcoDI GTCTC 4 cut(s) 27, 900, 1023, 1202
BcuI ACTAGT 1 cut(s) 752
BfaI CTAG 4 cut(s) 318, 501, 753, 1349
BfmI CTRYAG 1 cut(s) 1807
BfuAI ACCTGC 1 cut(s) 1172
BfuI GTATCC 1 cut(s) 1043
BisI GCNGC 1 cut(s) 69
BlpI GCTNAGC 2 cut(s) 343, 921
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 6 cut(s) 586, 738, 870, 960, 1149, 1664
Bme18I GGWCC 3 cut(s) 704, 1066, 1152
BmgT120I GGNCC 3 cut(s) 704, 1066, 1152
BmiI GGNNCC 8 cut(s) 330, 409, 531, 823, 1067, 1153, 1159, 1682
BmrFI CCNGG 6 cut(s) 586, 738, 870, 960, 1149, 1664
BmsI GCATC 3 cut(s) 1106, 1168, 1361
BpiI GAAGAC 2 cut(s) 210, 447
BpmI CTGGAG 4 cut(s) 270, 648, 853, 1327
Bpu10I CCTNAGC 1 cut(s) 1855
Bpu1102I GCTNAGC 2 cut(s) 343, 921
BpuEI CTTGAG 2 cut(s) 537, 1783
BpuMI CCSGG 2 cut(s) 960, 1664
BsaAI YACGTR 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 1815
BsaJI CCNNGG 2 cut(s) 1148, 1376
BsaWI WCCGGW 1 cut(s) 1154
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 831, 869, 1154
Bse1I ACTGG 5 cut(s) 48, 378, 403, 1069, 1310
Bse3DI GCAATG 2 cut(s) 569, 1092
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 4 cut(s) 586, 738, 870, 1149
BseDI CCNNGG 2 cut(s) 1148, 1376
BseGI GGATG 3 cut(s) 574, 744, 1444
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 3 cut(s) 831, 869, 1154
BseMI GCAATG 2 cut(s) 569, 1092
BseMII CTCAG 7 cut(s) 201, 357, 477, 912, 1197, 1221, 1311
BseNI ACTGG 5 cut(s) 48, 378, 403, 1069, 1310
BseRI GAGGAG 2 cut(s) 1647, 1650
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 1688
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 3 cut(s) 768, 775, 1521
BshNI GGYRCC 1 cut(s) 529
BsiEI CGRYCG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 959, 1155, 1664
BslFI GGGAC 5 cut(s) 1079, 1165, 1612, 1817, 1826
BslI CCNNNNNNNGG 3 cut(s) 831, 869, 1154
BsmAI GTCTC 4 cut(s) 27, 900, 1023, 1202
BsmFI GGGAC 5 cut(s) 1079, 1165, 1612, 1817, 1826
BsnI GGCC 3 cut(s) 768, 775, 1521
Bsp1286I GDGCHC 2 cut(s) 11, 1092
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
Bsp1720I GCTNAGC 2 cut(s) 343, 921
BspACI CCGC 1 cut(s) 702
BspANI GGCC 3 cut(s) 768, 775, 1521
BspCNI CTCAG 7 cut(s) 202, 356, 476, 913, 1198, 1222, 1312
BspHI TCATGA 3 cut(s) 151, 1132, 1696
BspLI GGNNCC 8 cut(s) 330, 409, 531, 823, 1067, 1153, 1159, 1682
BspMI ACCTGC 1 cut(s) 1172
BspPI GGATC 6 cut(s) 402, 415, 728, 1152, 1165, 1609
BspQI GCTCTTC 2 cut(s) 1446, 1754
BspT107I GGYRCC 1 cut(s) 529
BsrDI GCAATG 2 cut(s) 569, 1092
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 5 cut(s) 48, 378, 403, 1069, 1310
BssECI CCNNGG 2 cut(s) 1148, 1376
BssMI GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
BssNI GRCGYC 1 cut(s) 1815
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 1 cut(s) 1376
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 4 cut(s) 586, 738, 870, 1149
Bst4CI ACNGT 8 cut(s) 223, 580, 721, 947, 1062, 1105, 1603, 1838
Bst6I CTCTTC 2 cut(s) 1446, 1754
BstACI GRCGYC 1 cut(s) 1815
BstAPI GCANNNNNTGC 1 cut(s) 179
BstAUI TGTACA 1 cut(s) 786
BstBAI YACGTR 1 cut(s) 145
BstC8I GCNNGC 5 cut(s) 279, 1203, 1475, 1825, 1829
BstF5I GGATG 3 cut(s) 574, 744, 1444
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 9 cut(s) 410, 429, 662, 736, 982, 990, 1160, 1617, 1696
BstMAI GTCTC 4 cut(s) 27, 900, 1023, 1202
BstMBI GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 2 cut(s) 179, 1372
BstNI CCWGG 4 cut(s) 586, 738, 870, 1149
BstNSI RCATGY 5 cut(s) 364, 700, 1273, 1477, 1827
BstSCI CCNGG 6 cut(s) 584, 736, 868, 958, 1147, 1662
BstSFI CTRYAG 1 cut(s) 1807
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 2 cut(s) 210, 447
BstX2I RGATCY 3 cut(s) 407, 733, 1157
BstXI CCANNNNNNTGG 1 cut(s) 1595
BstYI RGATCY 3 cut(s) 407, 733, 1157
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 3 cut(s) 768, 775, 1521
BtsCI GGATG 3 cut(s) 574, 744, 1444
BtsI GCAGTG 1 cut(s) 1771
BtsIMutI CAGTG 5 cut(s) 385, 975, 1067, 1293, 1771
BveI ACCTGC 1 cut(s) 1172
Cac8I GCNNGC 5 cut(s) 279, 1203, 1475, 1825, 1829
CciI TCATGA 3 cut(s) 151, 1132, 1696
CfoI GCGC 1 cut(s) 184
Cfr13I GGNCC 3 cut(s) 704, 1066, 1152
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
DpnI GATC 9 cut(s) 409, 428, 661, 735, 981, 989, 1159, 1616, 1695
DpnII GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
EaeI YGGCCR 2 cut(s) 773, 1519
Eam1104I CTCTTC 2 cut(s) 1446, 1754
EarI CTCTTC 2 cut(s) 1446, 1754
Eco130I CCWWGG 1 cut(s) 1376
Eco147I AGGCCT 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 11, 1092
Eco47I GGWCC 3 cut(s) 704, 1066, 1152
EcoRII CCWGG 4 cut(s) 584, 736, 868, 1147
EcoT14I CCWWGG 1 cut(s) 1376
EcoT38I GRGCYC 2 cut(s) 11, 1092
ErhI CCWWGG 1 cut(s) 1376
FaqI GGGAC 5 cut(s) 1079, 1165, 1612, 1817, 1826
FauNDI CATATG 1 cut(s) 338
FbaI TGATCA 3 cut(s) 426, 979, 987
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 3 cut(s) 581, 731, 1451
FriOI GRGCYC 2 cut(s) 11, 1092
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 4 cut(s) 318, 501, 753, 1349
GlaI GCGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 69
GsuI CTGGAG 4 cut(s) 270, 648, 853, 1327
HaeIII GGCC 3 cut(s) 768, 775, 1521
HapII CCGG 3 cut(s) 959, 1155, 1664
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 1815
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HincII GTYRAC 1 cut(s) 1360
HindII GTYRAC 1 cut(s) 1360
HinfI GANTC 8 cut(s) 121, 206, 314, 374, 520, 878, 1561, 1784
HpaII CCGG 3 cut(s) 959, 1155, 1664
HphI GGTGA 3 cut(s) 107, 852, 1199
Hpy166II GTNNAC 8 cut(s) 142, 707, 789, 965, 1074, 1360, 1573, 1606
Hpy188I TCNGA 6 cut(s) 27, 211, 351, 525, 1420, 1654
Hpy188III TCNNGA 7 cut(s) 152, 249, 318, 465, 832, 1133, 1697
Hpy8I GTNNAC 8 cut(s) 142, 707, 789, 965, 1074, 1360, 1573, 1606
HpyAV CCTTC 8 cut(s) 190, 255, 421, 520, 1171, 1307, 1694, 1798
HpyCH4III ACNGT 8 cut(s) 223, 580, 721, 947, 1062, 1105, 1603, 1838
HpyCH4IV ACGT 3 cut(s) 144, 1776, 1815
HpyCH4V TGCA 8 cut(s) 58, 281, 1097, 1121, 1181, 1477, 1669, 1831
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 1372
HpySE526I ACGT 3 cut(s) 144, 1776, 1815
Hsp92I GRCGYC 1 cut(s) 1815
HspAI GCGC 1 cut(s) 182
Ksp22I TGATCA 3 cut(s) 426, 979, 987
Kzo9I GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
LguI GCTCTTC 2 cut(s) 1446, 1754
LmnI GCTCC 4 cut(s) 334, 352, 834, 1680
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 3 cut(s) 1106, 1168, 1361
MaeI CTAG 4 cut(s) 318, 501, 753, 1349
MaeII ACGT 3 cut(s) 144, 1776, 1815
MaeIII GTNAC 5 cut(s) 77, 217, 580, 1643, 1772
MalI GATC 9 cut(s) 409, 428, 661, 735, 981, 989, 1159, 1616, 1695
MboI GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
MboII GAAGA 4 cut(s) 215, 452, 1463, 1771
MflI RGATCY 3 cut(s) 407, 733, 1157
MhlI GDGCHC 2 cut(s) 11, 1092
MlsI TGGCCA 2 cut(s) 775, 1521
MluCI AATT 7 cut(s) 283, 485, 778, 949, 1331, 1531, 1655
MluNI TGGCCA 2 cut(s) 775, 1521
MlyI GAGTC 3 cut(s) 200, 308, 872
MmeI TCCRAC 2 cut(s) 1416, 1609
Mox20I TGGCCA 2 cut(s) 775, 1521
MscI TGGCCA 2 cut(s) 775, 1521
MseI TTAA 3 cut(s) 639, 761, 1728
MslI CAYNNNNRTG 5 cut(s) 93, 156, 337, 1281, 1533
Msp20I TGGCCA 2 cut(s) 775, 1521
MspI CCGG 3 cut(s) 959, 1155, 1664
MspR9I CCNGG 6 cut(s) 586, 738, 870, 960, 1149, 1664
MvaI CCWGG 4 cut(s) 586, 738, 870, 1149
MwoI GCNNNNNNNGC 2 cut(s) 179, 1372
NciI CCSGG 2 cut(s) 960, 1664
NdeI CATATG 1 cut(s) 338
NdeII GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
NlaIV GGNNCC 8 cut(s) 330, 409, 531, 823, 1067, 1153, 1159, 1682
NmuCI GTSAC 2 cut(s) 77, 1643
NspI RCATGY 5 cut(s) 364, 700, 1273, 1477, 1827
OliI CACNNNNGTG 1 cut(s) 1281
PaeI GCATGC 2 cut(s) 1477, 1827
PagI TCATGA 3 cut(s) 151, 1132, 1696
PaqCI CACCTGC 1 cut(s) 1172
PceI AGGCCT 1 cut(s) 768
PciI ACATGT 1 cut(s) 360
PciSI GCTCTTC 2 cut(s) 1446, 1754
PfeI GAWTC 5 cut(s) 121, 374, 520, 1561, 1784
PflMI CCANNNNNTGG 1 cut(s) 869
PfoI TCCNGGA 1 cut(s) 868
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 3 cut(s) 200, 308, 872
PpsI GAGTC 3 cut(s) 200, 308, 872
Ppu21I YACGTR 1 cut(s) 145
PscI ACATGT 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 584, 736, 868, 1147
PspGI CCWGG 4 cut(s) 584, 736, 868, 1147
PspN4I GGNNCC 8 cut(s) 330, 409, 531, 823, 1067, 1153, 1159, 1682
PspPI GGNCC 3 cut(s) 704, 1066, 1152
PsuI RGATCY 3 cut(s) 407, 733, 1157
RseI CAYNNNNRTG 5 cut(s) 93, 156, 337, 1281, 1533
SapI GCTCTTC 2 cut(s) 1446, 1754
SaqAI TTAA 3 cut(s) 639, 761, 1728
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 9 cut(s) 407, 426, 659, 733, 979, 987, 1157, 1614, 1693
Sau96I GGNCC 3 cut(s) 704, 1066, 1152
SchI GAGTC 3 cut(s) 200, 308, 872
ScrFI CCNGG 6 cut(s) 586, 738, 870, 960, 1149, 1664
SduI GDGCHC 2 cut(s) 11, 1092
SfaNI GCATC 3 cut(s) 1106, 1168, 1361
SfcI CTRYAG 1 cut(s) 1807
SinI GGWCC 3 cut(s) 704, 1066, 1152
SmiMI CAYNNNNRTG 5 cut(s) 93, 156, 337, 1281, 1533
SmlI CTYRAG 3 cut(s) 10, 552, 1798
SmoI CTYRAG 3 cut(s) 10, 552, 1798
SpeI ACTAGT 1 cut(s) 752
SphI GCATGC 2 cut(s) 1477, 1827
Sse9I AATT 7 cut(s) 283, 485, 778, 949, 1331, 1531, 1655
SseBI AGGCCT 1 cut(s) 768
SsiI CCGC 1 cut(s) 702
SspMI CTAG 4 cut(s) 318, 501, 753, 1349
StuI AGGCCT 1 cut(s) 768
StyD4I CCNGG 6 cut(s) 584, 736, 868, 958, 1147, 1662
StyI CCWWGG 1 cut(s) 1376
TaaI ACNGT 8 cut(s) 223, 580, 721, 947, 1062, 1105, 1603, 1838
TaiI ACGT 3 cut(s) 147, 1779, 1818
TaqI TCGA 4 cut(s) 296, 308, 658, 1086
TaqII GACCGA 2 cut(s) 326, 791
TasI AATT 7 cut(s) 283, 485, 778, 949, 1331, 1531, 1655
TatI WGTACW 5 cut(s) 357, 786, 1326, 1494, 1752
TfiI GAWTC 5 cut(s) 121, 374, 520, 1561, 1784
Tru1I TTAA 3 cut(s) 639, 761, 1728
Tru9I TTAA 3 cut(s) 639, 761, 1728
TscAI CASTG 5 cut(s) 385, 982, 1067, 1300, 1771
TseFI GTSAC 2 cut(s) 77, 1643
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 2 cut(s) 77, 1643
TspGWI ACGGA 1 cut(s) 1623
TspRI CASTG 5 cut(s) 385, 982, 1067, 1300, 1771
Van91I CCANNNNNTGG 1 cut(s) 869
VpaK11BI GGWCC 3 cut(s) 704, 1066, 1152
XapI RAATTY 4 cut(s) 283, 778, 1331, 1655
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 5 cut(s) 364, 700, 1273, 1477, 1827
XcmI CCANNNNNNNNNTGG 1 cut(s) 1625
XspI CTAG 4 cut(s) 318, 501, 753, 1349
ZraI GACGTC 1 cut(s) 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.