Rmu_sc0004886.1_g000021

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004886.1
OG0001351
Physical Location & Seq
Forward (+)
111315 .. 118429
7115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004886.1_g000021.1.cds

Sequence Viewer

Length: 3387 bp
atggacgtttattatatttacccaagttcgtcaaactcattgggagctagccttccccaagcacaaatggagggctcaagcttcttgtccctgaaagttgctctgatactggttctcttgttgcattacacagcagctcatagccacaataagcatgcaggcgacggggttcagccgctttccaaggttcaaatccacagagctgtttatgagcttcatgaaaatgcttccctcaaagcataccctgcacttcttgggatgaagggagaagattctgagtgggtaactgtgaaagttgatagtcctaatccttctggagatgattggttggcagttttttctcctgcaaatttcgactcatcgacttgtccatctaccgactccagacaacaggctccatacatatgctcagctccgatcaagtacatgtttgcgaatgattccagtgctgattatacaaagactggcaaaggaactttcaaacttcagttgatcaatcaaagggaagatttttcatttgcattgttctcaggcgggctatcaaacccaaaattggtggcagtttctagtgctataagtttcttaaatccgaaagcacccctttaccctcgtcttgctcaaggaaagcaatgggatgaaatgacagtaacctggacaagtggctataacattgatgaagctgtaccttttgttgagtggggtctaaagggagaggctcaaacccgatctccagcaggaacattgacttttacacgagagagcatgtgtggtccaccagctcgaacagttggttggcgcgatcctggtttcatccacactagtttcttaaaggacctttggccaaattcattgtacaattacaagctcggtcataggtcatctaatggttcctatatttggagcaagtcttactctttcaagtcatcaccatatcccggtcaggactcattgcagcgtgtagttatatttggagacatgggaaaggctgagcgcgatggttcaaatgaatacagtaattatcagccgggttcactcaatactactgatcaactgatcaaggacttagataacattgacatagttttccacataggagacatctcctatgccaatggatacatctcacagtgggaccagttcacatcacaggttgagcctattgcatcgactgtaccatatatgattgcaagtggtaatcatgaacgagatacaccagggaccggatccttctataacggtaatgatgcaggtggtgaatgtggtgtgctagctgagactatgttttatgttcctgccgagaaccgagctaagttctggtactcaacagattatggcatgttccacttctgtgtagctgacactgaacatgactggagggaaggaactgagcagtacaaatttattgagaaatgccttgcatcagttgacaggcagaaacagccttggctgatattcactgctcatcgtgttcttggttattcctccaacttatattatgtgttggatggttcatttgaagagccaatgggtagggaaagcatgcaaaagctatggcaaaagtacaaggtggatattgctttctatggccatgtccataactatgaaagaatatgccccatttaccagaatcaatgtgtaaacacagaaaaatcccattattctgggactgtgaatggaacaatccacattgttgttggaggaggaggcagtcacttatcagaatttagcccagtgcagaccacttggagccttcacaaagatcaagactttggctttgtaaaactcacagcatttaaccactcatcacttctcatggagtacaagaagagcagtgacggtaatgtctatgattccttcactatctcaagggactacagggacgtcttggcatgcgtgcatgacgttcatagtcacaataagcatggaggtgatggggtacagccactgtccaagattcaaatccagagagatgtttataagcttcatgaaaatgcttctgtcacggcaaacccagtccttcttggcaccaagggggaagattctcaatgggtaacggtggaagttggcactcccaagccttctgaagttgattggttggcagtcttttctcctgcaaatttcaagtttgccaatgattccaattctgattattcaaagacaggaaaaacatctttaaaatttcaattgatcaatcaaagggaagatttttcatttgcattattctccggtgggctgtcaaatccaaaactggtagctgtttctaatgttataacttttgcaaatccaaaggcacccctttacccccgtcttgctctagggcagcactgggatgaaatgacagtgacctggacaagcggctataatattgatgaagctgtaccttttgttgaatgggatatcaagggagagtctcaaactagctctccagcaggaacattaacttttactcgagagaccatgtgcggtccaccagcccgaacagttggctggcgcgatcctggtttcatccacacaggttttctgaaagatttgtacacttacaagcttggccatagtttatctaatagttcttatatctggagcaagtcctactctttcaagtcatcaccttatcctgggcaggactcattgcagcatgttgttatatttggtgacatgggaaaggccgagcgtgacggctctaacgaatatgctgattatcagcccggatctctcattaccagggatcaactggtcaaggagttggatggagttgacatagtttttcacataggagatatggcctattccaatggatacatctcacagtgggatcagttcacatcgcaggtgaagccgattgcatcaactgcaccatatatggttgcaaggtttggaaacatcattttgagatatacaactggtggtgaatgtggtgtgctagccgagactacgttctatgtgcctgctgagaacagagctaagtactcaacagattatggaaagttccacttctgcatagcagacagtgagcatgattggagagaaggttcagagctgtacaaattcattgagaaatgccttgcatcggctgacaggcagaaacagccttggctgattttcgctgctcatcgcgttcttggttattcctcggcttattggaatgatggttcttttggagaaccaatggcaagggaaaacttacaaaagctttggcagaagtgcaaggtggacattgcatttttcggccatatccacaactatgaaagaacatgcccaatttatcagaaccagtgtgtgaactcggaaacatctcattattccggaactgtgaatggaacaatccatgcagttgttggtggaggagctagggagccacttatcaggtttcggaccagtgcagtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

1128

Amino Acids

126.22

Weight (kDa)

5.93

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1977, 2270
AarI CACCTGC 2 cut(s) 1238, 2818
AasI GACNNNNNNGTC 1 cut(s) 1841
AatII GACGTC 1 cut(s) 1884
Acc36I ACCTGC 2 cut(s) 1238, 2818
AccB1I GGYRCC 2 cut(s) 2024, 2290
AccII CGCG 4 cut(s) 798, 993, 2493, 3114
AccIII TCCGGA 1 cut(s) 3302
AciI CCGC 4 cut(s) 176, 534, 2355, 2463
AclWI GGATC 7 cut(s) 794, 1218, 1231, 2489, 2716, 2733, 2820
AcoI YGGCCR 4 cut(s) 839, 1585, 2548, 3226
AcsI RAATTY 7 cut(s) 349, 844, 1397, 1721, 2116, 2177, 3044
AcuI CTGAAG 2 cut(s) 470, 2103
AcyI GRCGYC 1 cut(s) 1881
AfiI CCNNNNNNNGG 6 cut(s) 553, 897, 935, 1220, 2615, 3067
AflIII ACRYGT 1 cut(s) 426
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 7 cut(s) 650, 802, 1213, 2345, 2497, 2614, 2720
AjuI GAANNNNNNNTTGG 2 cut(s) 775, 807
AleI CACNNNNGTG 1 cut(s) 1347
AloI GAACNNNNNNTCC 4 cut(s) 3013, 3045, 3133, 3165
Alw26I GTCTC 6 cut(s) 966, 1089, 1268, 2415, 2447, 2921
AlwI GGATC 7 cut(s) 794, 1218, 1231, 2489, 2716, 2733, 2820
AlwNI CAGNNNCTG 1 cut(s) 1945
Ama87I CYCGRG 1 cut(s) 2448
Aor13HI TCCGGA 1 cut(s) 3302
AoxI GGCC 6 cut(s) 839, 1585, 2548, 2664, 2781, 3226
ApeKI GCWGC 5 cut(s) 134, 952, 2320, 2632, 3104
ApoI RAATTY 7 cut(s) 349, 844, 1397, 1721, 2116, 2177, 3044
ArsI GACNNNNNNTTYG 2 cut(s) 1757, 1789
AspLEI GCGC 3 cut(s) 798, 993, 2493
AspS9I GGNCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
AsuC2I CCSGG 3 cut(s) 936, 1026, 2706
AsuHPI GGTGA 7 cut(s) 918, 1265, 1940, 2598, 2663, 2842, 2918
AsuNHI GCTAGC 3 cut(s) 47, 1267, 2920
AvaI CYCGRG 1 cut(s) 2448
AvaII GGWCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
BalI TGGCCA 3 cut(s) 841, 1587, 2550
BamHI GGATCC 1 cut(s) 1223
BanI GGYRCC 2 cut(s) 2024, 2290
BanII GRGCYC 1 cut(s) 77
BauI CACGAG 1 cut(s) 753
BbvI GCAGC 5 cut(s) 146, 964, 2332, 2644, 3091
BccI CCATC 6 cut(s) 379, 989, 1499, 1925, 2741, 3140
BceAI ACGGC 2 cut(s) 2019, 2692
BciT130I CCWGG 7 cut(s) 652, 804, 1215, 2347, 2499, 2616, 2722
BciVI GTATCC 2 cut(s) 1109, 2789
BclI TGATCA 4 cut(s) 492, 1045, 1053, 2187
BcnI CCSGG 3 cut(s) 936, 1026, 2706
BcoDI GTCTC 6 cut(s) 966, 1089, 1268, 2415, 2447, 2921
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 8 cut(s) 48, 567, 819, 1268, 2315, 2418, 2921, 3348
BfmI CTRYAG 1 cut(s) 1873
BfuAI ACCTGC 2 cut(s) 1238, 2818
BfuI GTATCC 2 cut(s) 1109, 2789
BisI GCNGC 7 cut(s) 135, 176, 953, 2321, 2356, 2633, 3105
BlpI GCTNAGC 2 cut(s) 409, 987
BlsI GCNGC 7 cut(s) 136, 177, 954, 2322, 2357, 2634, 3106
BmcAI AGTACT 1 cut(s) 2966
Bme18I GGWCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
BmeT110I CYCGRG 1 cut(s) 2448
BmgT120I GGNCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
BmiI GGNNCC 9 cut(s) 396, 889, 1133, 1219, 1225, 1748, 2026, 2292, 3354
BmrI ACTGGG 3 cut(s) 1724, 2006, 2335
BmsI GCATC 5 cut(s) 1172, 1234, 1427, 2852, 3074
BmtI GCTAGC 3 cut(s) 51, 1271, 2924
BmuI ACTGGG 3 cut(s) 1724, 2006, 2335
BpmI CTGGAG 6 cut(s) 336, 367, 714, 1393, 2409, 2599
Bpu1102I GCTNAGC 2 cut(s) 409, 987
BpuEI CTTGAG 3 cut(s) 61, 603, 1849
BpuMI CCSGG 3 cut(s) 936, 1026, 2706
BsaBI GATNNNNATC 1 cut(s) 1958
BsaHI GRCGYC 1 cut(s) 1881
BsaI GGTCTC 1 cut(s) 2447
BsaJI CCNNGG 8 cut(s) 183, 1214, 1442, 2028, 2615, 2721, 3089, 3129
BsaWI WCCGGW 3 cut(s) 1220, 2225, 3302
BsaXI ACNNNNNCTCC 6 cut(s) 712, 742, 2407, 2437, 3013, 3043
Bsc4I CCNNNNNNNGG 6 cut(s) 553, 897, 935, 1220, 2615, 3067
Bse3DI GCAATG 4 cut(s) 635, 947, 2627, 3213
Bse8I GATNNNNATC 1 cut(s) 1958
BseAI TCCGGA 1 cut(s) 3302
BseBI CCWGG 7 cut(s) 652, 804, 1215, 2347, 2499, 2616, 2722
BseDI CCNNGG 8 cut(s) 183, 1214, 1442, 2028, 2615, 2721, 3089, 3129
BseGI GGATG 7 cut(s) 264, 640, 810, 1510, 2335, 2505, 2752
BseJI GATNNNNATC 1 cut(s) 1958
BseLI CCNNNNNNNGG 6 cut(s) 553, 897, 935, 1220, 2615, 3067
BseMI GCAATG 4 cut(s) 635, 947, 2627, 3213
BseMII CTCAG 7 cut(s) 267, 423, 543, 978, 1263, 1377, 2940
BseRI GAGGAG 3 cut(s) 1713, 1716, 3357
BseXI GCAGC 5 cut(s) 146, 964, 2332, 2644, 3091
BsgI GTGCAG 3 cut(s) 231, 1754, 2835
Bsh1236I CGCG 4 cut(s) 798, 993, 2493, 3114
BshFI GGCC 6 cut(s) 841, 1587, 2550, 2666, 2783, 3228
BshNI GGYRCC 2 cut(s) 2024, 2290
BsiHKCI CYCGRG 1 cut(s) 2448
BsiSI CCGG 6 cut(s) 936, 1025, 1221, 2226, 2706, 3303
BslFI GGGAC 6 cut(s) 73, 1145, 1231, 1678, 1883, 1892
BslI CCNNNNNNNGG 6 cut(s) 553, 897, 935, 1220, 2615, 3067
BsmAI GTCTC 6 cut(s) 966, 1089, 1268, 2415, 2447, 2921
BsmFI GGGAC 6 cut(s) 73, 1145, 1231, 1678, 1883, 1892
BsnI GGCC 6 cut(s) 841, 1587, 2550, 2666, 2783, 3228
Bso31I GGTCTC 1 cut(s) 2447
BsoBI CYCGRG 1 cut(s) 2448
Bsp1286I GDGCHC 1 cut(s) 77
Bsp13I TCCGGA 1 cut(s) 3302
Bsp1407I TGTACA 3 cut(s) 852, 2532, 3039
Bsp1720I GCTNAGC 2 cut(s) 409, 987
BspACI CCGC 4 cut(s) 176, 534, 2355, 2463
BspANI GGCC 6 cut(s) 841, 1587, 2550, 2666, 2783, 3228
BspCNI CTCAG 7 cut(s) 268, 422, 542, 979, 1264, 1378, 2941
BspEI TCCGGA 1 cut(s) 3302
BspFNI CGCG 4 cut(s) 798, 993, 2493, 3114
BspHI TCATGA 3 cut(s) 217, 1198, 1984
BspLI GGNNCC 9 cut(s) 396, 889, 1133, 1219, 1225, 1748, 2026, 2292, 3354
BspMI ACCTGC 2 cut(s) 1238, 2818
BspOI GCTAGC 3 cut(s) 51, 1271, 2924
BspPI GGATC 7 cut(s) 794, 1218, 1231, 2489, 2716, 2733, 2820
BspQI GCTCTTC 2 cut(s) 1512, 1820
BspT107I GGYRCC 2 cut(s) 2024, 2290
BspTNI GGTCTC 1 cut(s) 2447
BsrDI GCAATG 4 cut(s) 635, 947, 2627, 3213
BsrGI TGTACA 3 cut(s) 852, 2532, 3039
BssECI CCNNGG 8 cut(s) 183, 1214, 1442, 2028, 2615, 2721, 3089, 3129
BssNI GRCGYC 1 cut(s) 1881
BssSI CACGAG 1 cut(s) 753
BssT1I CCWWGG 4 cut(s) 183, 1442, 2028, 3089
Bst2BI CACGAG 1 cut(s) 753
Bst2UI CCWGG 7 cut(s) 652, 804, 1215, 2347, 2499, 2616, 2722
Bst6I CTCTTC 2 cut(s) 1512, 1820
BstACI GRCGYC 1 cut(s) 1881
BstAPI GCANNNNNTGC 2 cut(s) 245, 2849
BstAUI TGTACA 3 cut(s) 852, 2532, 3039
BstF5I GGATG 7 cut(s) 264, 640, 810, 1510, 2335, 2505, 2752
BstFNI CGCG 4 cut(s) 798, 993, 2493, 3114
BstHHI GCGC 3 cut(s) 798, 993, 2493
BstMAI GTCTC 6 cut(s) 966, 1089, 1268, 2415, 2447, 2921
BstMWI GCNNNNNNNGC 5 cut(s) 245, 1438, 2833, 2849, 3085
BstNI CCWGG 7 cut(s) 652, 804, 1215, 2347, 2499, 2616, 2722
BstNSI RCATGY 8 cut(s) 158, 430, 766, 1339, 1543, 1893, 2639, 3255
BstSFI CTRYAG 1 cut(s) 1873
BstUI CGCG 4 cut(s) 798, 993, 2493, 3114
BstV1I GCAGC 5 cut(s) 146, 964, 2332, 2644, 3091
BstX2I RGATCY 2 cut(s) 1223, 2708
BstXI CCANNNNNNTGG 1 cut(s) 1661
BstYI RGATCY 2 cut(s) 1223, 2708
BsuI GTATCC 2 cut(s) 1109, 2789
BsuRI GGCC 6 cut(s) 841, 1587, 2550, 2666, 2783, 3228
BtgZI GCGATG 3 cut(s) 1008, 2808, 3095
BtsCI GGATG 7 cut(s) 264, 640, 810, 1510, 2335, 2505, 2752
BtsI GCAGTG 3 cut(s) 1455, 1837, 3387
BveI ACCTGC 2 cut(s) 1238, 2818
CaiI CAGNNNCTG 1 cut(s) 1945
CciI TCATGA 3 cut(s) 217, 1198, 1984
CfoI GCGC 3 cut(s) 798, 993, 2493
Cfr13I GGNCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
DraI TTTAAA 1 cut(s) 2175
DrdI GACNNNNNNGTC 1 cut(s) 1841
DseDI GACNNNNNNGTC 1 cut(s) 1841
EaeI YGGCCR 4 cut(s) 839, 1585, 2548, 3226
Eam1104I CTCTTC 2 cut(s) 1512, 1820
EarI CTCTTC 2 cut(s) 1512, 1820
Eco130I CCWWGG 4 cut(s) 183, 1442, 2028, 3089
Eco24I GRGCYC 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 2447
Eco32I GATATC 1 cut(s) 2398
Eco47I GGWCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
Eco57I CTGAAG 2 cut(s) 470, 2103
Eco88I CYCGRG 1 cut(s) 2448
EcoO109I RGGNCCY 1 cut(s) 832
EcoRII CCWGG 7 cut(s) 650, 802, 1213, 2345, 2497, 2614, 2720
EcoRV GATATC 1 cut(s) 2398
EcoT14I CCWWGG 4 cut(s) 183, 1442, 2028, 3089
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 4 cut(s) 183, 1442, 2028, 3089
FalI AAGNNNNNCTT 4 cut(s) 585, 617, 2975, 3007
FaqI GGGAC 6 cut(s) 73, 1145, 1231, 1678, 1883, 1892
FauI CCCGC 1 cut(s) 527
FauNDI CATATG 1 cut(s) 404
FbaI TGATCA 4 cut(s) 492, 1045, 1053, 2187
Fnu4HI GCNGC 7 cut(s) 135, 176, 953, 2321, 2356, 2633, 3105
FokI GGATG 7 cut(s) 271, 647, 797, 1517, 2342, 2492, 2759
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 7 cut(s) 135, 176, 953, 2321, 2356, 2633, 3105
FspBI CTAG 8 cut(s) 48, 567, 819, 1268, 2315, 2418, 2921, 3348
GlaI GCGC 3 cut(s) 797, 992, 2492
GluI GCNGC 7 cut(s) 135, 176, 953, 2321, 2356, 2633, 3105
GsuI CTGGAG 6 cut(s) 336, 367, 714, 1393, 2409, 2599
HaeIII GGCC 6 cut(s) 841, 1587, 2550, 2666, 2783, 3228
HapII CCGG 6 cut(s) 936, 1025, 1221, 2226, 2706, 3303
HhaI GCGC 3 cut(s) 798, 993, 2493
Hin1I GRCGYC 1 cut(s) 1881
Hin6I GCGC 3 cut(s) 796, 991, 2491
HinP1I GCGC 3 cut(s) 796, 991, 2491
HincII GTYRAC 2 cut(s) 1426, 2755
HindII GTYRAC 2 cut(s) 1426, 2755
HindIII AAGCTT 4 cut(s) 79, 1979, 2543, 3188
HpaII CCGG 6 cut(s) 936, 1025, 1221, 2226, 2706, 3303
HphI GGTGA 7 cut(s) 918, 1265, 1940, 2598, 2663, 2842, 2918
Hpy99I CGWCG 1 cut(s) 167
HpyCH4IV ACGT 4 cut(s) 6, 1881, 1902, 2933
HpyF10VI GCNNNNNNNGC 5 cut(s) 245, 1438, 2833, 2849, 3085
HpySE526I ACGT 4 cut(s) 6, 1881, 1902, 2933
Hsp92I GRCGYC 1 cut(s) 1881
HspAI GCGC 3 cut(s) 796, 991, 2491
Kpn2I TCCGGA 1 cut(s) 3302
Ksp22I TGATCA 4 cut(s) 492, 1045, 1053, 2187
LguI GCTCTTC 2 cut(s) 1512, 1820
LmnI GCTCC 8 cut(s) 44, 400, 418, 900, 1746, 2580, 3344, 3352
Lsp1109I GCAGC 5 cut(s) 146, 964, 2332, 2644, 3091
LweI GCATC 5 cut(s) 1172, 1234, 1427, 2852, 3074
MaeI CTAG 8 cut(s) 48, 567, 819, 1268, 2315, 2418, 2921, 3348
MaeII ACGT 4 cut(s) 6, 1881, 1902, 2933
MboII GAAGA 6 cut(s) 281, 518, 1529, 1837, 2048, 2213
MfeI CAATTG 1 cut(s) 2183
MflI RGATCY 2 cut(s) 1223, 2708
MhlI GDGCHC 1 cut(s) 77
MlsI TGGCCA 3 cut(s) 841, 1587, 2550
MluNI TGGCCA 3 cut(s) 841, 1587, 2550
MlyI GAGTC 5 cut(s) 350, 374, 938, 2417, 2618
MmeI TCCRAC 4 cut(s) 1482, 1509, 1675, 2724
Mox20I TGGCCA 3 cut(s) 841, 1587, 2550
MroI TCCGGA 1 cut(s) 3302
MscI TGGCCA 3 cut(s) 841, 1587, 2550
MseI TTAA 5 cut(s) 584, 827, 1794, 2174, 2438
MslI CAYNNNNRTG 8 cut(s) 222, 403, 1347, 1599, 1926, 1989, 2328, 3240
Msp20I TGGCCA 3 cut(s) 841, 1587, 2550
MspI CCGG 6 cut(s) 936, 1025, 1221, 2226, 2706, 3303
MunI CAATTG 1 cut(s) 2183
MvaI CCWGG 7 cut(s) 652, 804, 1215, 2347, 2499, 2616, 2722
MvnI CGCG 4 cut(s) 798, 993, 2493, 3114
MwoI GCNNNNNNNGC 5 cut(s) 245, 1438, 2833, 2849, 3085
NciI CCSGG 3 cut(s) 936, 1026, 2706
NdeI CATATG 1 cut(s) 404
NheI GCTAGC 3 cut(s) 47, 1267, 2920
NlaIV GGNNCC 9 cut(s) 396, 889, 1133, 1219, 1225, 1748, 2026, 2292, 3354
NmeAIII GCCGAG 4 cut(s) 1321, 2692, 2950, 3110
NmuCI GTSAC 7 cut(s) 1709, 1832, 1910, 1999, 2341, 2651, 2672
NspI RCATGY 8 cut(s) 158, 430, 766, 1339, 1543, 1893, 2639, 3255
OliI CACNNNNGTG 1 cut(s) 1347
PaeI GCATGC 3 cut(s) 158, 1543, 1893
PaeR7I CTCGAG 1 cut(s) 2448
PagI TCATGA 3 cut(s) 217, 1198, 1984
PaqCI CACCTGC 2 cut(s) 1238, 2818
PciI ACATGT 1 cut(s) 426
PciSI GCTCTTC 2 cut(s) 1512, 1820
PcsI WCGNNNNNNNCGW 1 cut(s) 2682
PfeI GAWTC 7 cut(s) 272, 440, 1627, 1850, 1954, 2039, 2135
PkrI GCNGC 7 cut(s) 136, 177, 954, 2322, 2357, 2634, 3106
PleI GAGTC 5 cut(s) 350, 374, 938, 2416, 2618
PpsI GAGTC 5 cut(s) 350, 374, 938, 2416, 2618
PpuMI RGGWCCY 1 cut(s) 832
PscI ACATGT 1 cut(s) 426
PsiI TTATAA 2 cut(s) 1977, 2270
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 7 cut(s) 650, 802, 1213, 2345, 2497, 2614, 2720
PspGI CCWGG 7 cut(s) 650, 802, 1213, 2345, 2497, 2614, 2720
PspN4I GGNNCC 9 cut(s) 396, 889, 1133, 1219, 1225, 1748, 2026, 2292, 3354
PspPI GGNCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
PspPPI RGGWCCY 1 cut(s) 832
PstNI CAGNNNCTG 1 cut(s) 1945
PsuI RGATCY 2 cut(s) 1223, 2708
RseI CAYNNNNRTG 8 cut(s) 222, 403, 1347, 1599, 1926, 1989, 2328, 3240
SapI GCTCTTC 2 cut(s) 1512, 1820
SaqAI TTAA 5 cut(s) 584, 827, 1794, 2174, 2438
SatI GCNGC 7 cut(s) 135, 176, 953, 2321, 2356, 2633, 3105
Sau96I GGNCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
ScaI AGTACT 1 cut(s) 2966
SchI GAGTC 5 cut(s) 350, 374, 938, 2417, 2618
SduI GDGCHC 1 cut(s) 77
SfaNI GCATC 5 cut(s) 1172, 1234, 1427, 2852, 3074
SfcI CTRYAG 1 cut(s) 1873
Sfr274I CTCGAG 1 cut(s) 2448
SinI GGWCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
SlaI CTCGAG 1 cut(s) 2448
SmiMI CAYNNNNRTG 8 cut(s) 222, 403, 1347, 1599, 1926, 1989, 2328, 3240
SmlI CTYRAG 4 cut(s) 76, 618, 1864, 2448
SmoI CTYRAG 4 cut(s) 76, 618, 1864, 2448
SpeI ACTAGT 1 cut(s) 818
SphI GCATGC 3 cut(s) 158, 1543, 1893
SsiI CCGC 4 cut(s) 176, 534, 2355, 2463
SspI AATATT 1 cut(s) 2365
SspMI CTAG 8 cut(s) 48, 567, 819, 1268, 2315, 2418, 2921, 3348
StyI CCWWGG 4 cut(s) 183, 1442, 2028, 3089
TaiI ACGT 4 cut(s) 9, 1884, 1905, 2936
TaqI TCGA 5 cut(s) 354, 362, 781, 1166, 2449
TaqII GACCGA 1 cut(s) 857
TatI WGTACW 8 cut(s) 423, 852, 1392, 1560, 1818, 2532, 2964, 3039
TauI GCSGC 2 cut(s) 178, 2358
TfiI GAWTC 7 cut(s) 272, 440, 1627, 1850, 1954, 2039, 2135
Tru1I TTAA 5 cut(s) 584, 827, 1794, 2174, 2438
Tru9I TTAA 5 cut(s) 584, 827, 1794, 2174, 2438
TseFI GTSAC 7 cut(s) 1709, 1832, 1910, 1999, 2341, 2651, 2672
TseI GCWGC 5 cut(s) 134, 952, 2320, 2632, 3104
Tsp45I GTSAC 7 cut(s) 1709, 1832, 1910, 1999, 2341, 2651, 2672
VpaK11BI GGWCC 6 cut(s) 770, 832, 1132, 1218, 2465, 3372
XapI RAATTY 7 cut(s) 349, 844, 1397, 1721, 2116, 2177, 3044
XceI RCATGY 8 cut(s) 158, 430, 766, 1339, 1543, 1893, 2639, 3255
XcmI CCANNNNNNNNNTGG 3 cut(s) 1691, 2728, 3332
XhoI CTCGAG 1 cut(s) 2448
XspI CTAG 8 cut(s) 48, 567, 819, 1268, 2315, 2418, 2921, 3348
ZraI GACGTC 1 cut(s) 1882
ZrmI AGTACT 1 cut(s) 2966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.