Rw7G009420

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001351
Physical Location & Seq
Forward (+)
8828722 .. 8834126
5405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009420.1

Sequence Viewer

Length: 1287 bp
ATGCAGCCAAAACTGGTAGCTGTTTCTAATGTTATAACTTTTGCAAATCCAAAGGCACCCCTTTACCCCCGTCTTGCTCTAGGGCAGCACTGGGATGAAATGACAGTGACCTGGACAAGCGGCTATAATATTGATGAAGCTGTACCTTTTGTTGAATGGGGTATCAAGGGAGAGTCTCAAACTAGCTCTCCAGCAGGAACATTAACTTTTACTCGAGAGACCATGTGCGGTCCACCAGCCCGAACAGTTGGCTGGCGCGATCCTGGTTTCATCCACACAGGTTTTCTGAAAGATTTGTGGCCAAATTCTTTGTACACTTACAAGCTTGGCCATAGTTTATCTAATAGTTCTTATATCTGGAGCAAGTCCTACTCTTTCAAGTCATCACCTTATCCTGGGCAGGACTCATTGCAGCATGTTGTTATATTTGGTGACATGGGAAAGAAAAAATTGCGTGACGGCTCTAACGAATATGCTGATTATCAGCCCGGATCTCTCATTACCAGGGATCAACTGGTCAAGGAGTTGGATGGAGTTGACATAGTTTTTCACATAGGAGATATGGCCTATTCCAATGGATACATCTCACAGTGGGATCAGTTCACATCGCAGGTGAAGCCGATTGCATCAACTGTACCATATATGGGTGGAGAATGTGGTGTGCTAGCCGAGACTATGTTCTATGTGCCTGCTGAGAACAGAGCTAAGTTCTGGTACTCAACAGATTATGGAAAGTTCCACTTCTGCATAGCAGACAGTGAGCATGATTGGAGAGAAGGTTCAGAGCTGTACAAATTCATTGAGAAATGCCTTGCATCGGCTGACAGGCAGAAACAGCCTTGGCTGATTTTCGCTGCTCATCGCGTTCTTGGTTATTCCTCGGCTTATTGGAATGATGGTTCTTTTGGAGAACAAATGGCAAGGGAAAACTTACAAAAGCTTTGGCAGAAGTGCAAGGTGGACATTGCATTTTTCGGCCATATCCACAACTATGAAAGAACATGCCCAATTTATCAGAACCAGTGTGTGAACTCGGAAACATCTCATTATTCCGGAACTGTGAATGGAACAATCCATGCAGTTGTTGGTGGAGGAGCTAGGGAGCCACTTATCAGGTTTCGGACCAGTGCAACCACTCGGAGCCTTTTCAAAGATTCCGATTTCAGCTTCACGAAGCTCACTGCATTTAAGTCTATGATTCCTTCACTATCTCAAGGGACTACAGGGATCAGTGATGTCTTGGCTTGTGTACATGATGGTTGTGAACCAACTACTCTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

428

Amino Acids

48.03

Weight (kDa)

6.36

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 22 - 127 1.1e-13 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 140 - 331 4.2e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AarI CACCTGC 1 cut(s) 601
Acc36I ACCTGC 1 cut(s) 601
AccB1I GGYRCC 1 cut(s) 55
AccII CGCG 2 cut(s) 258, 864
AccIII TCCGGA 1 cut(s) 1052
AciI CCGC 2 cut(s) 120, 228
AclWI GGATC 5 cut(s) 254, 499, 516, 603, 1235
AcoI YGGCCR 3 cut(s) 299, 328, 976
AcsI RAATTY 2 cut(s) 304, 794
AfaI GTAC 6 cut(s) 144, 314, 636, 716, 791, 1251
AfiI CCNNNNNNNGG 3 cut(s) 395, 644, 817
AgsI TTSAA 3 cut(s) 155, 379, 1150
AjnI CCWGG 4 cut(s) 110, 262, 394, 503
AloI GAACNNNNNNTCC 4 cut(s) 763, 795, 883, 915
Alw26I GTCTC 3 cut(s) 180, 212, 665
AlwI GGATC 5 cut(s) 254, 499, 516, 603, 1235
Ama87I CYCGRG 1 cut(s) 213
Aor13HI TCCGGA 1 cut(s) 1052
AoxI GGCC 4 cut(s) 299, 328, 564, 976
ApeKI GCWGC 4 cut(s) 4, 85, 412, 854
ApoI RAATTY 2 cut(s) 304, 794
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 230, 1122
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 3 cut(s) 378, 443, 625
AsuNHI GCTAGC 1 cut(s) 664
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 2 cut(s) 230, 1122
BalI TGGCCA 2 cut(s) 301, 330
BanI GGYRCC 1 cut(s) 55
BbvI GCAGC 4 cut(s) 16, 97, 424, 841
BccI CCATC 3 cut(s) 524, 890, 1250
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 4 cut(s) 112, 264, 396, 505
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 180, 212, 665
BfaI CTAG 5 cut(s) 80, 183, 665, 1098, 1277
BfmI CTRYAG 1 cut(s) 1221
BfuAI ACCTGC 1 cut(s) 601
BfuI GTATCC 1 cut(s) 572
BisI GCNGC 5 cut(s) 5, 86, 121, 413, 855
BlsI GCNGC 5 cut(s) 6, 87, 122, 414, 856
Bme1390I CCNGG 5 cut(s) 112, 264, 396, 489, 505
Bme18I GGWCC 2 cut(s) 230, 1122
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 230, 1122
BmiI GGNNCC 3 cut(s) 57, 1104, 1142
BmrFI CCNGG 5 cut(s) 112, 264, 396, 489, 505
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 2 cut(s) 635, 824
BmtI GCTAGC 1 cut(s) 668
BmuI ACTGGG 1 cut(s) 100
BpmI CTGGAG 2 cut(s) 174, 379
BpuEI CTTGAG 1 cut(s) 1197
BpuMI CCSGG 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 395, 504, 839, 879
BsaWI WCCGGW 1 cut(s) 1052
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 763, 793
Bsc4I CCNNNNNNNGG 3 cut(s) 395, 644, 817
Bse1I ACTGG 5 cut(s) 18, 95, 519, 1021, 1125
Bse3DI GCAATG 2 cut(s) 407, 963
BseAI TCCGGA 1 cut(s) 1052
BseBI CCWGG 4 cut(s) 112, 264, 396, 505
BseDI CCNNGG 4 cut(s) 395, 504, 839, 879
BseGI GGATG 3 cut(s) 100, 270, 535
BseLI CCNNNNNNNGG 3 cut(s) 395, 644, 817
BseMI GCAATG 2 cut(s) 407, 963
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 5 cut(s) 18, 95, 519, 1021, 1125
BseRI GAGGAG 1 cut(s) 1107
BseXI GCAGC 4 cut(s) 16, 97, 424, 841
Bsh1236I CGCG 2 cut(s) 258, 864
BshFI GGCC 4 cut(s) 301, 330, 566, 978
BshNI GGYRCC 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 489, 1053
BslFI GGGAC 1 cut(s) 1231
BslI CCNNNNNNNGG 3 cut(s) 395, 644, 817
BsmAI GTCTC 3 cut(s) 180, 212, 665
BsmFI GGGAC 1 cut(s) 1231
BsnI GGCC 4 cut(s) 301, 330, 566, 978
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 1052
Bsp1407I TGTACA 3 cut(s) 312, 789, 1249
Bsp143I GATC 5 cut(s) 259, 491, 508, 595, 1227
BspACI CCGC 2 cut(s) 120, 228
BspANI GGCC 4 cut(s) 301, 330, 566, 978
BspCNI CTCAG 1 cut(s) 685
BspEI TCCGGA 1 cut(s) 1052
BspFNI CGCG 2 cut(s) 258, 864
BspLI GGNNCC 3 cut(s) 57, 1104, 1142
BspMI ACCTGC 1 cut(s) 601
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 5 cut(s) 254, 499, 516, 603, 1235
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 2 cut(s) 407, 963
BsrGI TGTACA 3 cut(s) 312, 789, 1249
BsrI ACTGG 5 cut(s) 18, 95, 519, 1021, 1125
BssECI CCNNGG 4 cut(s) 395, 504, 839, 879
BssMI GATC 5 cut(s) 259, 491, 508, 595, 1227
BssT1I CCWWGG 1 cut(s) 839
Bst2UI CCWGG 4 cut(s) 112, 264, 396, 505
Bst4CI ACNGT 6 cut(s) 106, 247, 591, 634, 758, 1060
BstAUI TGTACA 3 cut(s) 312, 789, 1249
BstC8I GCNNGC 3 cut(s) 254, 666, 690
BstDEI CTNAG 2 cut(s) 693, 705
BstF5I GGATG 3 cut(s) 100, 270, 535
BstFNI CGCG 2 cut(s) 258, 864
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 5 cut(s) 262, 494, 511, 598, 1230
BstMAI GTCTC 3 cut(s) 180, 212, 665
BstMBI GATC 5 cut(s) 259, 491, 508, 595, 1227
BstMWI GCNNNNNNNGC 2 cut(s) 616, 835
BstNI CCWGG 4 cut(s) 112, 264, 396, 505
BstNSI RCATGY 2 cut(s) 419, 1005
BstSCI CCNGG 5 cut(s) 110, 262, 394, 487, 503
BstSFI CTRYAG 1 cut(s) 1221
BstUI CGCG 2 cut(s) 258, 864
BstV1I GCAGC 4 cut(s) 16, 97, 424, 841
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 4 cut(s) 301, 330, 566, 978
BtgZI GCGATG 2 cut(s) 591, 845
BtsCI GGATG 3 cut(s) 100, 270, 535
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 8 cut(s) 88, 111, 596, 763, 1028, 1132, 1179, 1237
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 3 cut(s) 254, 666, 690
CfoI GCGC 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 230, 1122
Csp6I GTAC 6 cut(s) 143, 313, 635, 715, 790, 1250
CviAII CATG 8 cut(s) 223, 416, 436, 764, 1002, 1076, 1253, 1284
CviQI GTAC 6 cut(s) 143, 313, 635, 715, 790, 1250
DdeI CTNAG 2 cut(s) 693, 705
DpnI GATC 5 cut(s) 261, 493, 510, 597, 1229
DpnII GATC 5 cut(s) 259, 491, 508, 595, 1227
EaeI YGGCCR 3 cut(s) 299, 328, 976
Eco130I CCWWGG 1 cut(s) 839
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 230, 1122
Eco88I CYCGRG 1 cut(s) 213
EcoRII CCWGG 4 cut(s) 110, 262, 394, 503
EcoT14I CCWWGG 1 cut(s) 839
ErhI CCWWGG 1 cut(s) 839
FaeI CATG 8 cut(s) 226, 419, 439, 767, 1005, 1079, 1256, 1287
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 1 cut(s) 1231
FatI CATG 8 cut(s) 222, 415, 435, 763, 1001, 1075, 1252, 1283
Fnu4HI GCNGC 5 cut(s) 5, 86, 121, 413, 855
FokI GGATG 3 cut(s) 107, 257, 542
Fsp4HI GCNGC 5 cut(s) 5, 86, 121, 413, 855
FspBI CTAG 5 cut(s) 80, 183, 665, 1098, 1277
GlaI GCGC 1 cut(s) 257
GluI GCNGC 5 cut(s) 5, 86, 121, 413, 855
GsuI CTGGAG 2 cut(s) 174, 379
HaeIII GGCC 4 cut(s) 301, 330, 566, 978
HapII CCGG 2 cut(s) 489, 1053
HhaI GCGC 1 cut(s) 258
Hin1II CATG 8 cut(s) 226, 419, 439, 767, 1005, 1079, 1256, 1287
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 2 cut(s) 323, 938
HinfI GANTC 4 cut(s) 173, 404, 1154, 1198
HpaII CCGG 2 cut(s) 489, 1053
HphI GGTGA 3 cut(s) 378, 443, 625
Hpy166II GTNNAC 8 cut(s) 233, 315, 538, 603, 961, 1030, 1250, 1265
Hpy188I TCNGA 7 cut(s) 288, 784, 1017, 1036, 1122, 1140, 1159
Hpy188III TCNNGA 4 cut(s) 215, 358, 1053, 1171
Hpy8I GTNNAC 8 cut(s) 233, 315, 538, 603, 961, 1030, 1250, 1265
HpyAV CCTTC 2 cut(s) 770, 1212
HpyCH4III ACNGT 6 cut(s) 106, 247, 591, 634, 758, 1060
HpyF10VI GCNNNNNNNGC 2 cut(s) 616, 835
HpyF3I CTNAG 2 cut(s) 693, 705
Hsp92II CATG 8 cut(s) 226, 419, 439, 767, 1005, 1079, 1256, 1287
HspAI GCGC 1 cut(s) 256
Kpn2I TCCGGA 1 cut(s) 1052
Kzo9I GATC 5 cut(s) 259, 491, 508, 595, 1227
LmnI GCTCC 4 cut(s) 360, 1094, 1102, 1140
Lsp1109I GCAGC 4 cut(s) 16, 97, 424, 841
LweI GCATC 2 cut(s) 635, 824
MaeI CTAG 5 cut(s) 80, 183, 665, 1098, 1277
MaeIII GTNAC 3 cut(s) 106, 431, 455
MalI GATC 5 cut(s) 261, 493, 510, 597, 1229
MboI GATC 5 cut(s) 259, 491, 508, 595, 1227
MflI RGATCY 1 cut(s) 491
MlsI TGGCCA 2 cut(s) 301, 330
MluCI AATT 4 cut(s) 304, 449, 794, 1008
MluNI TGGCCA 2 cut(s) 301, 330
MlyI GAGTC 2 cut(s) 182, 398
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 2 cut(s) 889, 1085
Mox20I TGGCCA 2 cut(s) 301, 330
MroI TCCGGA 1 cut(s) 1052
MscI TGGCCA 2 cut(s) 301, 330
MseI TTAA 2 cut(s) 203, 1188
MslI CAYNNNNRTG 2 cut(s) 93, 990
Msp20I TGGCCA 2 cut(s) 301, 330
MspI CCGG 2 cut(s) 489, 1053
MspR9I CCNGG 5 cut(s) 112, 264, 396, 489, 505
MvaI CCWGG 4 cut(s) 112, 264, 396, 505
MvnI CGCG 2 cut(s) 258, 864
MwoI GCNNNNNNNGC 2 cut(s) 616, 835
NciI CCSGG 1 cut(s) 489
NdeII GATC 5 cut(s) 259, 491, 508, 595, 1227
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 8 cut(s) 226, 419, 439, 767, 1005, 1079, 1256, 1287
NlaIV GGNNCC 3 cut(s) 57, 1104, 1142
NmeAIII GCCGAG 2 cut(s) 694, 860
NmuCI GTSAC 3 cut(s) 106, 431, 455
NspI RCATGY 2 cut(s) 419, 1005
PaeR7I CTCGAG 1 cut(s) 213
PaqCI CACCTGC 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PfeI GAWTC 2 cut(s) 1154, 1198
PkrI GCNGC 5 cut(s) 6, 87, 122, 414, 856
PleI GAGTC 2 cut(s) 181, 398
PpsI GAGTC 2 cut(s) 181, 398
PsiI TTATAA 1 cut(s) 35
Psp6I CCWGG 4 cut(s) 110, 262, 394, 503
PspGI CCWGG 4 cut(s) 110, 262, 394, 503
PspN4I GGNNCC 3 cut(s) 57, 1104, 1142
PspPI GGNCC 2 cut(s) 230, 1122
PsuI RGATCY 1 cut(s) 491
RsaI GTAC 6 cut(s) 144, 314, 636, 716, 791, 1251
RsaNI GTAC 6 cut(s) 143, 313, 635, 715, 790, 1250
RseI CAYNNNNRTG 2 cut(s) 93, 990
SaqAI TTAA 2 cut(s) 203, 1188
SatI GCNGC 5 cut(s) 5, 86, 121, 413, 855
Sau3AI GATC 5 cut(s) 259, 491, 508, 595, 1227
Sau96I GGNCC 2 cut(s) 230, 1122
SchI GAGTC 2 cut(s) 182, 398
ScrFI CCNGG 5 cut(s) 112, 264, 396, 489, 505
SfaNI GCATC 2 cut(s) 635, 824
SfcI CTRYAG 1 cut(s) 1221
Sfr274I CTCGAG 1 cut(s) 213
SinI GGWCC 2 cut(s) 230, 1122
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 2 cut(s) 93, 990
SmlI CTYRAG 2 cut(s) 213, 1212
SmoI CTYRAG 2 cut(s) 213, 1212
Sse9I AATT 4 cut(s) 304, 449, 794, 1008
SsiI CCGC 2 cut(s) 120, 228
SspI AATATT 1 cut(s) 130
SspMI CTAG 5 cut(s) 80, 183, 665, 1098, 1277
StyD4I CCNGG 5 cut(s) 110, 262, 394, 487, 503
StyI CCWWGG 1 cut(s) 839
TaaI ACNGT 6 cut(s) 106, 247, 591, 634, 758, 1060
TaqI TCGA 1 cut(s) 214
TasI AATT 4 cut(s) 304, 449, 794, 1008
TatI WGTACW 3 cut(s) 312, 789, 1249
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 2 cut(s) 1154, 1198
Tru1I TTAA 2 cut(s) 203, 1188
Tru9I TTAA 2 cut(s) 203, 1188
TscAI CASTG 8 cut(s) 95, 111, 596, 763, 1028, 1132, 1186, 1237
TseFI GTSAC 3 cut(s) 106, 431, 455
TseI GCWGC 4 cut(s) 4, 85, 412, 854
Tsp45I GTSAC 3 cut(s) 106, 431, 455
TspDTI ATGAA 5 cut(s) 111, 150, 259, 787, 1008
TspRI CASTG 8 cut(s) 95, 111, 596, 763, 1028, 1132, 1186, 1237
VpaK11BI GGWCC 2 cut(s) 230, 1122
XapI RAATTY 2 cut(s) 304, 794
XceI RCATGY 2 cut(s) 419, 1005
XcmI CCANNNNNNNNNTGG 2 cut(s) 511, 1082
XhoI CTCGAG 1 cut(s) 213
XspI CTAG 5 cut(s) 80, 183, 665, 1098, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.