RchiOBHm_Chr7g0190261

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001351
Physical Location & Seq
Forward (+)
9403246 .. 9405821
2576 bp
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UTR
Exon/CDS
Intron
PRQ16991

Sequence Viewer

Length: 1551 bp
ATGCACATCTCCTATAAAGGTATTTGTTATTTGCTTGTTGGATACAAGTTTGCCAATTATTCCAGGGCTGATTATGCAAACACAGGCAATACAACTTTGAAGTTCCGATTGATCAATCAACGGGCAGATTTTTCGTTTGCATTGTTCTCGGGCGGGTTGTCAAACCCGAAATTGGTGGCAGTTTCTAATAATGTAACCTTTGCGTATCTGAAGGCTCCCCTTTACCCTCGTCTTGCTCTAGGGAAGCTCTGGGATGAAATGACAGTGACTTGGACAAGTGGATATAACATCGATGAAGCTGTACCTGTAGTTGAGTGGGGTTTAAAGGGAGAACCTCAAACTCGATCTCCAGCAGGAACATTGACTTTCAGTAGACGGGACATGTGTGCTGCACCTGCACGAACAGTTGGTTGGCGTGATCCTGGTTTCATCCATACAAGTTTTCTAAAAGATTTGTGGCCAAATTCAGTGTACTCTTACAAGCTTGGTCATAGGTTATCTAATGGTTCCTATATCTGGAGCAAGGCTTACTCTTTCAAGTCATCACCATATCCTGGGCAGGAGTCATTGCAGCGTGTTATTATATTTGGTGACATGGGAAAAGCTGAGCGTGATGGTTCAAATGAATATGCTGATTATCAGCCCGGCTCACTCAATACCACAGATCAGCTAATCAAGGATTTGGACAACATTGACATAGTTTTTCATATAGGAGATATGCCTTATGCCAATGGATACATCTCACAGTGGGATCAGTTCACATCACAGGTGGAGCCGATTTCATCAGTTGTACCATATATGGTTGCCAGTGGCAACCATGAACGAGATACAACAGGGACCGGATCCTTCTACGACACTAACGATGCAGGTGGTGAATGTGGTGTGCCAGCTGAGACCATGTATTATGTTCCTGCTGAGAACAGAGCTAAATTCTGGTATGCAACAGATTATGGTATGTTTCACTTCTGTATAGCTGACACCGAGCATGACTGGAGAGAAGGTTCTGAGCAGTACAAATTTATTGAGAAATGCCTTGCATCTGCCAACAGGCACAAGCAGCCTTGGCTGATATTCGCTGCTCATCGTGTTCTCGGTTATTCCTCTAATTCTTGGTATGGACAAGAGGGTTCATTTGACGAGCCGACGGGAAGGGATGACATGCAAAAGCTTTGGCAAAGATACAAGGTGGACATTGCATTTTACGGCCATGTCCATAACTACGAAAGAATATGCCCCATCTATCAGAACCAGTGTGTAACTTCGGAAATATCTAACTATTCAGGAACTGTCAACGGAACAATCCATGTTGTTGTTGGTGGAGGAGGCAGCCACTTATCAGATTTTAGCTCGCTGCACACAGTTTGGAGCATTTACCGAGATCGAGATTATGGTTATACGAAACTCACAGCATATAACCACTCATCGCTCCTATTTGAGTACTTGAAGAGCAGCGATGGAAAGGTCTATGATTCATTCACCATCTCAAGGGACTACAAGGATGTCTTGGCCTGTGTTCATGATGGTTGTGAACCAACCACCTTAGCTACATGA

Protein Analysis

516

Amino Acids

58.36

Weight (kDa)

5.89

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 7 - 68 7.2e-20 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 76 - 180 1e-12 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 192 - 407 8.4e-17 Calcineurin-like phosphoesterase
Metallophos_C PF14008 432 - 491 1.2e-16 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 403, 857
Acc36I ACCTGC 2 cut(s) 403, 857
AccB7I CCANNNNNTGG 1 cut(s) 554
AccI GTMKAC 1 cut(s) 373
AciI CCGC 1 cut(s) 153
AclWI GGATC 4 cut(s) 413, 759, 837, 850
AcoI YGGCCR 2 cut(s) 458, 1204
AcsI RAATTY 3 cut(s) 463, 929, 1016
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 5 cut(s) 303, 473, 792, 1013, 1439
AfiI CCNNNNNNNGG 4 cut(s) 172, 516, 554, 1485
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 4 cut(s) 100, 538, 621, 1444
AjnI CCWGG 3 cut(s) 62, 421, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AloI GAACNNNNNNTCC 2 cut(s) 985, 1017
Alw26I GTCTC 1 cut(s) 887
AlwI GGATC 4 cut(s) 413, 759, 837, 850
AlwNI CAGNNNCTG 1 cut(s) 1286
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 3 cut(s) 458, 1204, 1506
ApeKI GCWGC 7 cut(s) 389, 571, 1057, 1076, 1326, 1351, 1449
ApoI RAATTY 3 cut(s) 463, 929, 1016
AspS9I GGNCC 1 cut(s) 837
AsuC2I CCSGG 1 cut(s) 645
AsuHPI GGTGA 4 cut(s) 537, 602, 884, 1468
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 837
BalI TGGCCA 1 cut(s) 460
BamHI GGATCC 1 cut(s) 842
BbvI GCAGC 7 cut(s) 376, 583, 1063, 1069, 1338, 1338, 1461
BccI CCATC 5 cut(s) 608, 1244, 1448, 1487, 1514
BceAI ACGGC 1 cut(s) 1219
BcgI CGANNNNNNTGC 4 cut(s) 114, 129, 148, 163
BciT130I CCWGG 3 cut(s) 64, 423, 555
BciVI GTATCC 2 cut(s) 35, 728
BclI TGATCA 1 cut(s) 111
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 887
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 1 cut(s) 306
BfuAI ACCTGC 2 cut(s) 403, 857
BfuI GTATCC 2 cut(s) 35, 728
BisI GCNGC 7 cut(s) 390, 572, 1058, 1077, 1327, 1352, 1450
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 7 cut(s) 391, 573, 1059, 1078, 1328, 1353, 1451
BmcAI AGTACT 1 cut(s) 1439
Bme1390I CCNGG 4 cut(s) 64, 423, 555, 645
Bme18I GGWCC 1 cut(s) 837
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 837
BmiI GGNNCC 5 cut(s) 216, 508, 774, 838, 844
BmrFI CCNGG 4 cut(s) 64, 423, 555, 645
BmsI GCATC 2 cut(s) 853, 1046
BpmI CTGGAG 3 cut(s) 333, 538, 1012
Bpu10I CCTNAGC 1 cut(s) 1540
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 1468
BpuMI CCSGG 1 cut(s) 645
Bsa29I ATCGAT 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 887
BsaJI CCNNGG 3 cut(s) 63, 554, 1061
BsaWI WCCGGW 1 cut(s) 839
BsaXI ACNNNNNCTCC 4 cut(s) 331, 361, 985, 1015
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 516, 554, 1485
Bse1I ACTGG 3 cut(s) 807, 995, 1249
Bse3DI GCAATG 2 cut(s) 566, 1191
BseBI CCWGG 3 cut(s) 64, 423, 555
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 63, 554, 1061
BseGI GGATG 4 cut(s) 259, 429, 1159, 1504
BseLI CCNNNNNNNGG 4 cut(s) 172, 516, 554, 1485
BseMI GCAATG 2 cut(s) 566, 1191
BseMII CTCAG 4 cut(s) 597, 882, 906, 996
BseNI ACTGG 3 cut(s) 807, 995, 1249
BseRI GAGGAG 1 cut(s) 1335
BseXI GCAGC 7 cut(s) 376, 583, 1063, 1069, 1338, 1338, 1461
BsgI GTGCAG 3 cut(s) 375, 381, 1337
BshFI GGCC 3 cut(s) 460, 1206, 1508
BshVI ATCGAT 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 2 cut(s) 645, 840
BslFI GGGAC 3 cut(s) 392, 850, 1502
BslI CCNNNNNNNGG 4 cut(s) 172, 516, 554, 1485
BsmAI GTCTC 1 cut(s) 887
BsmFI GGGAC 3 cut(s) 392, 850, 1502
BsnI GGCC 3 cut(s) 460, 1206, 1508
Bso31I GGTCTC 1 cut(s) 887
BsoBI CYCGRG 1 cut(s) 148
Bsp143I GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 1 cut(s) 153
BspANI GGCC 3 cut(s) 460, 1206, 1508
BspCNI CTCAG 4 cut(s) 598, 883, 907, 997
BspDI ATCGAT 1 cut(s) 291
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 5 cut(s) 216, 508, 774, 838, 844
BspMI ACCTGC 2 cut(s) 403, 857
BspPI GGATC 4 cut(s) 413, 759, 837, 850
BspQI GCTCTTC 1 cut(s) 1439
BspTNI GGTCTC 1 cut(s) 887
BsrDI GCAATG 2 cut(s) 566, 1191
BsrI ACTGG 3 cut(s) 807, 995, 1249
BssECI CCNNGG 3 cut(s) 63, 554, 1061
BssMI GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
BssT1I CCWWGG 1 cut(s) 1061
Bst2UI CCWGG 3 cut(s) 64, 423, 555
Bst4CI ACNGT 5 cut(s) 265, 406, 747, 1288, 1360
Bst6I CTCTTC 1 cut(s) 1439
BstC8I GCNNGC 2 cut(s) 888, 1349
BstDEI CTNAG 5 cut(s) 606, 891, 915, 1005, 1540
BstF5I GGATG 4 cut(s) 259, 429, 1159, 1504
BstKTI GATC 7 cut(s) 114, 347, 421, 667, 754, 845, 1381
BstMAI GTCTC 1 cut(s) 887
BstMBI GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
BstMWI GCNNNNNNNGC 4 cut(s) 74, 395, 1057, 1063
BstNI CCWGG 3 cut(s) 64, 423, 555
BstNSI RCATGY 2 cut(s) 385, 1162
BstSCI CCNGG 4 cut(s) 62, 421, 553, 643
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 7 cut(s) 376, 583, 1063, 1069, 1338, 1338, 1461
BstX2I RGATCY 1 cut(s) 842
BstYI RGATCY 1 cut(s) 842
Bsu15I ATCGAT 1 cut(s) 291
BsuI GTATCC 2 cut(s) 35, 728
BsuRI GGCC 3 cut(s) 460, 1206, 1508
BsuTUI ATCGAT 1 cut(s) 291
BtgZI GCGATG 2 cut(s) 1407, 1467
BtsCI GGATG 4 cut(s) 259, 429, 1159, 1504
BtsIMutI CAGTG 5 cut(s) 270, 474, 752, 814, 1256
BveI ACCTGC 2 cut(s) 403, 857
Cac8I GCNNGC 2 cut(s) 888, 1349
CaiI CAGNNNCTG 1 cut(s) 1286
CciI TCATGA 1 cut(s) 1516
Cfr13I GGNCC 1 cut(s) 837
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 5 cut(s) 302, 472, 791, 1012, 1438
CviQI GTAC 5 cut(s) 302, 472, 791, 1012, 1438
DdeI CTNAG 5 cut(s) 606, 891, 915, 1005, 1540
DpnI GATC 7 cut(s) 113, 346, 420, 666, 753, 844, 1380
DpnII GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
DraI TTTAAA 1 cut(s) 324
EaeI YGGCCR 2 cut(s) 458, 1204
Eam1104I CTCTTC 1 cut(s) 1439
EarI CTCTTC 1 cut(s) 1439
Eco130I CCWWGG 1 cut(s) 1061
Eco31I GGTCTC 1 cut(s) 887
Eco47I GGWCC 1 cut(s) 837
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 148
EcoRII CCWGG 3 cut(s) 62, 421, 553
EcoT14I CCWWGG 1 cut(s) 1061
ErhI CCWWGG 1 cut(s) 1061
FalI AAGNNNNNCTT 2 cut(s) 1487, 1519
FaqI GGGAC 3 cut(s) 392, 850, 1502
FauI CCCGC 1 cut(s) 146
FbaI TGATCA 1 cut(s) 111
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 7 cut(s) 390, 572, 1058, 1077, 1327, 1352, 1450
FokI GGATG 4 cut(s) 266, 416, 1166, 1511
Fsp4HI GCNGC 7 cut(s) 390, 572, 1058, 1077, 1327, 1352, 1450
FspBI CTAG 1 cut(s) 239
GluI GCNGC 7 cut(s) 390, 572, 1058, 1077, 1327, 1352, 1450
GsuI CTGGAG 3 cut(s) 333, 538, 1012
HaeIII GGCC 3 cut(s) 460, 1206, 1508
HapII CCGG 2 cut(s) 645, 840
HincII GTYRAC 1 cut(s) 1291
HindII GTYRAC 1 cut(s) 1291
HindIII AAGCTT 2 cut(s) 482, 1166
HinfI GANTC 2 cut(s) 563, 1469
HpaII CCGG 2 cut(s) 645, 840
HphI GGTGA 4 cut(s) 537, 602, 884, 1468
Hpy166II GTNNAC 6 cut(s) 374, 472, 759, 1189, 1291, 1529
Hpy188I TCNGA 6 cut(s) 107, 210, 1006, 1245, 1264, 1339
Hpy188III TCNNGA 4 cut(s) 517, 1281, 1382, 1517
Hpy8I GTNNAC 6 cut(s) 374, 472, 759, 1189, 1291, 1529
Hpy99I CGWCG 1 cut(s) 1147
HpyAV CCTTC 4 cut(s) 205, 856, 992, 1143
HpyCH4III ACNGT 5 cut(s) 265, 406, 747, 1288, 1360
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 395, 1057, 1063
HpyF3I CTNAG 5 cut(s) 606, 891, 915, 1005, 1540
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
LguI GCTCTTC 1 cut(s) 1439
LmnI GCTCC 5 cut(s) 220, 519, 772, 1365, 1431
Lsp1109I GCAGC 7 cut(s) 376, 583, 1063, 1069, 1338, 1338, 1461
LweI GCATC 2 cut(s) 853, 1046
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 4 cut(s) 193, 265, 590, 1255
MalI GATC 7 cut(s) 113, 346, 420, 666, 753, 844, 1380
MboI GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
MboII GAAGA 1 cut(s) 1456
MflI RGATCY 1 cut(s) 842
MlsI TGGCCA 1 cut(s) 460
MluCI AATT 6 cut(s) 55, 170, 463, 929, 1016, 1105
MluNI TGGCCA 1 cut(s) 460
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 6 cut(s) 237, 345, 1111, 1117, 1313, 1316
Mox20I TGGCCA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 460
MseI TTAA 1 cut(s) 323
Msp20I TGGCCA 1 cut(s) 460
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 2 cut(s) 645, 840
MspR9I CCNGG 4 cut(s) 64, 423, 555, 645
MvaI CCWGG 3 cut(s) 64, 423, 555
MwoI GCNNNNNNNGC 4 cut(s) 74, 395, 1057, 1063
NciI CCSGG 1 cut(s) 645
NdeII GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
NlaIV GGNNCC 5 cut(s) 216, 508, 774, 838, 844
NmuCI GTSAC 2 cut(s) 265, 590
NspI RCATGY 2 cut(s) 385, 1162
PagI TCATGA 1 cut(s) 1516
PaqCI CACCTGC 2 cut(s) 403, 857
PciI ACATGT 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 1439
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PfeI GAWTC 1 cut(s) 1469
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 7 cut(s) 391, 573, 1059, 1078, 1328, 1353, 1451
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
PscI ACATGT 1 cut(s) 381
Psp6I CCWGG 3 cut(s) 62, 421, 553
PspGI CCWGG 3 cut(s) 62, 421, 553
PspN4I GGNNCC 5 cut(s) 216, 508, 774, 838, 844
PspPI GGNCC 1 cut(s) 837
PstNI CAGNNNCTG 1 cut(s) 1286
PsuI RGATCY 1 cut(s) 842
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 5 cut(s) 303, 473, 792, 1013, 1439
RsaNI GTAC 5 cut(s) 302, 472, 791, 1012, 1438
SapI GCTCTTC 1 cut(s) 1439
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 7 cut(s) 390, 572, 1058, 1077, 1327, 1352, 1450
Sau3AI GATC 7 cut(s) 111, 344, 418, 664, 751, 842, 1378
Sau96I GGNCC 1 cut(s) 837
ScaI AGTACT 1 cut(s) 1439
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 4 cut(s) 64, 423, 555, 645
SfaNI GCATC 2 cut(s) 853, 1046
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 837
SmlI CTYRAG 1 cut(s) 1483
SmoI CTYRAG 1 cut(s) 1483
Sse9I AATT 6 cut(s) 55, 170, 463, 929, 1016, 1105
SsiI CCGC 1 cut(s) 153
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 4 cut(s) 62, 421, 553, 643
StyI CCWWGG 1 cut(s) 1061
TaaI ACNGT 5 cut(s) 265, 406, 747, 1288, 1360
TaqI TCGA 3 cut(s) 291, 343, 1381
TasI AATT 6 cut(s) 55, 170, 463, 929, 1016, 1105
TatI WGTACW 3 cut(s) 471, 1011, 1437
TfiI GAWTC 1 cut(s) 1469
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 5 cut(s) 270, 474, 752, 814, 1256
TseFI GTSAC 2 cut(s) 265, 590
TseI GCWGC 7 cut(s) 389, 571, 1057, 1076, 1326, 1351, 1449
Tsp45I GTSAC 2 cut(s) 265, 590
TspGWI ACGGA 1 cut(s) 1308
TspRI CASTG 5 cut(s) 270, 474, 752, 814, 1256
Van91I CCANNNNNTGG 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 837
XapI RAATTY 3 cut(s) 463, 929, 1016
XceI RCATGY 2 cut(s) 385, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 1310
XmiI GTMKAC 1 cut(s) 373
XspI CTAG 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.